                         SEQUENCE LISTING

<110>  Global Bioenergies 
       Scientist of Fortune S.A.
 
<120>  Methods for the enzymatic production of isoprene

<130>  U1624 PCT S3

<150>  EP 12 19 2428.6
<151>  2012-11-13

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  262
<212>  PRT
<213>  Escherichia coli (strain K12)

<400>  1

Met Gln Val Asp Leu Leu Gly Ser Ala Gln Ser Ala His Ala Leu His 
1               5                   10                  15      


Leu Phe His Gln His Ser Pro Leu Val His Cys Met Thr Asn Asp Val 
            20                  25                  30          


Val Gln Thr Phe Thr Ala Asn Thr Leu Leu Ala Leu Gly Ala Ser Pro 
        35                  40                  45              


Ala Met Val Ile Glu Thr Glu Glu Ala Ser Gln Phe Ala Ala Ile Ala 
    50                  55                  60                  


Ser Ala Leu Leu Ile Asn Val Gly Thr Leu Thr Gln Pro Arg Ala Gln 
65                  70                  75                  80  


Ala Met Arg Ala Ala Val Glu Gln Ala Lys Ser Ser Gln Thr Pro Trp 
                85                  90                  95      


Thr Leu Asp Pro Val Ala Val Gly Ala Leu Asp Tyr Arg Arg His Phe 
            100                 105                 110         


Cys His Glu Leu Leu Ser Phe Lys Pro Ala Ala Ile Arg Gly Asn Ala 
        115                 120                 125             


Ser Glu Ile Met Ala Leu Ala Gly Ile Ala Asn Gly Gly Arg Gly Val 
    130                 135                 140                 


Asp Thr Thr Asp Ala Ala Ala Asn Ala Ile Pro Ala Ala Gln Thr Leu 
145                 150                 155                 160 


Ala Arg Glu Thr Gly Ala Ile Val Val Val Thr Gly Glu Met Asp Tyr 
                165                 170                 175     


Val Thr Asp Gly His Arg Ile Ile Gly Ile His Gly Gly Asp Pro Leu 
            180                 185                 190         


Met Thr Lys Val Val Gly Thr Gly Cys Ala Leu Ser Ala Val Val Ala 
        195                 200                 205             


Ala Cys Cys Ala Leu Pro Gly Asp Thr Leu Glu Asn Val Ala Ser Ala 
    210                 215                 220                 


Cys His Trp Met Lys Gln Ala Gly Glu Arg Ala Val Ala Arg Ser Glu 
225                 230                 235                 240 


Gly Pro Gly Ser Phe Val Pro His Phe Leu Asp Ala Leu Trp Gln Leu 
                245                 250                 255     


Thr Gln Glu Val Gln Ala 
            260         


<210>  2
<211>  272
<212>  PRT
<213>  Bacillus subtilis

<400>  2

Met Asp Ala Gln Ser Ala Ala Lys Cys Leu Thr Ala Val Arg Arg His 
1               5                   10                  15      


Ser Pro Leu Val His Ser Ile Thr Asn Asn Val Val Thr Asn Phe Thr 
            20                  25                  30          


Ala Asn Gly Leu Leu Ala Leu Gly Ala Ser Pro Val Met Ala Tyr Ala 
        35                  40                  45              


Lys Glu Glu Val Ala Asp Met Ala Lys Ile Ala Gly Ala Leu Val Leu 
    50                  55                  60                  


Asn Ile Gly Thr Leu Ser Lys Glu Ser Val Glu Ala Met Ile Ile Ala 
65                  70                  75                  80  


Gly Lys Ser Ala Asn Glu His Gly Val Pro Val Ile Leu Asp Pro Val 
                85                  90                  95      


Gly Ala Gly Ala Thr Pro Phe Arg Thr Glu Ser Ala Arg Asp Ile Ile 
            100                 105                 110         


Arg Glu Val Arg Leu Ala Ala Ile Arg Gly Asn Ala Ala Glu Ile Ala 
        115                 120                 125             


His Thr Val Gly Val Thr Asp Trp Leu Ile Lys Gly Val Asp Ala Gly 
    130                 135                 140                 


Glu Gly Gly Gly Asp Ile Ile Arg Leu Ala Gln Gln Ala Ala Gln Lys 
145                 150                 155                 160 


Leu Asn Thr Val Ile Ala Ile Thr Gly Glu Val Asp Val Ile Ala Asp 
                165                 170                 175     


Thr Ser His Val Tyr Thr Leu His Asn Gly His Lys Leu Leu Thr Lys 
            180                 185                 190         


Val Thr Gly Ala Gly Cys Leu Leu Thr Ser Val Val Gly Ala Phe Cys 
        195                 200                 205             


Ala Val Glu Glu Asn Pro Leu Phe Ala Ala Ile Ala Ala Ile Ser Ser 
    210                 215                 220                 


Tyr Gly Val Ala Ala Gln Leu Ala Ala Gln Gln Thr Ala Asp Lys Gly 
225                 230                 235                 240 


Pro Gly Ser Phe Gln Ile Glu Leu Leu Asn Lys Leu Ser Thr Val Thr 
                245                 250                 255     


Glu Gln Asp Val Gln Glu Trp Ala Thr Ile Glu Arg Val Thr Val Ser 
            260                 265                 270         


<210>  3
<211>  267
<212>  PRT
<213>  Rhizobium leguminosarum bv. viciae (strain 3841)

<400>  3

Met Gln Thr Arg Thr Thr Pro Gly Ala Met Leu Lys Ala Met Arg Glu 
1               5                   10                  15      


Lys Pro Pro Leu Val Gln Cys Ile Thr Asn Tyr Val Ala Met Asn Ile 
            20                  25                  30          


Ala Ala Asn Val Leu Leu Ala Ala Gly Ala Ser Pro Ala Met Val His 
        35                  40                  45              


Ala Ala Glu Glu Ala Gly Glu Phe Ala Ala Ile Ala Ser Ala Leu Thr 
    50                  55                  60                  


Ile Asn Ile Gly Thr Leu Ser Thr Gln Trp Ile Asp Gly Met Gln Ala 
65                  70                  75                  80  


Ala Ala Lys Ala Ala Thr Ser Ala Gly Lys Pro Trp Val Leu Asp Pro 
                85                  90                  95      


Val Ala His Tyr Ala Thr Ala Phe Arg Arg Asn Ala Val Ala Glu Leu 
            100                 105                 110         


Leu Ala Leu Lys Pro Thr Ile Ile Arg Gly Asn Ala Ser Glu Ile Ile 
        115                 120                 125             


Ala Leu Ala Gly Gly Glu Ser Arg Gly Gln Gly Val Asp Ser Arg Asp 
    130                 135                 140                 


Pro Val Glu Gln Ala Glu Gly Ser Ala Arg Trp Leu Ala Glu Arg Gln 
145                 150                 155                 160 


Arg Ala Val Val Ala Val Thr Gly Ala Val Asp Phe Val Thr Asp Gly 
                165                 170                 175     


Glu Arg Ala Val Arg Ile Glu Gly Gly Ser Ala Leu Met Pro Gln Val 
            180                 185                 190         


Thr Ala Leu Gly Cys Ser Leu Thr Cys Leu Val Gly Ala Phe Ala Ala 
        195                 200                 205             


Thr Ala Pro Glu Asp Ile Phe Gly Ala Thr Val Ala Ala Leu Ser Thr 
    210                 215                 220                 


Phe Ala Ile Ala Gly Glu Glu Ala Ala Leu Gly Ala Ala Gly Pro Gly 
225                 230                 235                 240 


Ser Phe Ser Trp Arg Phe Leu Asp Ala Leu Ala Ala Leu Asp Ala Glu 
                245                 250                 255     


Thr Leu Asp Ala Arg Ala Arg Ile Ser Ala Ala 
            260                 265         


<210>  4
<211>  602
<212>  PRT
<213>  Populus trichocarpa

<400>  4

Met Ala Thr Glu Leu Leu Cys Leu His Arg Pro Ile Ser Leu Thr His 
1               5                   10                  15      


Lys Leu Phe Arg Asn Pro Leu Pro Lys Val Ile Gln Ala Thr Pro Leu 
            20                  25                  30          


Thr Leu Lys Leu Arg Cys Ser Val Ser Thr Glu Asn Val Ser Phe Thr 
        35                  40                  45              


Glu Thr Glu Thr Glu Thr Arg Arg Ser Ala Asn Tyr Glu Pro Asn Ser 
    50                  55                  60                  


Trp Asp Tyr Asp Tyr Leu Leu Ser Ser Asp Thr Asp Glu Ser Arg Lys 
65                  70                  75                  80  


Gly Arg Ser Ser Val Ile Glu Val Tyr Lys Asp Lys Ala Lys Lys Leu 
                85                  90                  95      


Glu Ala Glu Val Arg Arg Glu Ile Asn Asn Glu Lys Ala Glu Phe Leu 
            100                 105                 110         


Thr Leu Leu Glu Leu Ile Asp Asn Val Gln Arg Leu Gly Leu Gly Tyr 
        115                 120                 125             


Arg Phe Glu Ser Asp Ile Arg Arg Ala Leu Asp Arg Phe Val Ser Ser 
    130                 135                 140                 


Gly Gly Phe Asp Ala Val Thr Lys Thr Ser Leu His Ala Thr Ala Leu 
145                 150                 155                 160 


Ser Phe Arg Leu Leu Arg Gln His Gly Phe Glu Val Ser Gln Glu Ala 
                165                 170                 175     


Phe Gly Gly Phe Lys Asp Gln Asn Gly Asn Phe Met Glu Asn Leu Lys 
            180                 185                 190         


Glu Asp Ile Lys Ala Ile Leu Ser Leu Tyr Glu Ala Ser Phe Leu Ala 
        195                 200                 205             


Leu Glu Gly Glu Asn Ile Leu Asp Glu Ala Lys Val Phe Ala Ile Ser 
    210                 215                 220                 


His Leu Lys Glu Leu Ser Glu Glu Lys Ile Gly Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Gln Val Asn His Ala Leu Glu Leu Pro Leu His Arg Arg Thr Gln Arg 
                245                 250                 255     


Leu Glu Ala Val Leu Ser Ile Glu Ala Tyr Arg Lys Lys Glu Asp Ala 
            260                 265                 270         


Asp Gln Val Leu Leu Glu Leu Ala Ile Leu Asp Tyr Asn Met Ile Gln 
        275                 280                 285             


Ser Val Tyr Gln Arg Asp Leu Arg Glu Thr Ser Arg Trp Trp Arg Arg 
    290                 295                 300                 


Val Gly Leu Ala Thr Lys Leu His Phe Ala Arg Asp Arg Leu Ile Glu 
305                 310                 315                 320 


Ser Phe Tyr Trp Ala Val Gly Val Ala Phe Glu Pro Gln Tyr Ser Asp 
                325                 330                 335     


Cys Arg Asn Ser Val Ala Lys Met Phe Ser Phe Val Thr Ile Ile Asp 
            340                 345                 350         


Asp Ile Tyr Asp Val Tyr Gly Thr Leu Asp Glu Leu Glu Leu Phe Thr 
        355                 360                 365             


Asn Ala Val Glu Arg Trp Asp Val Asn Ala Ile Asp Asp Leu Pro Asp 
    370                 375                 380                 


Tyr Met Lys Leu Cys Phe Leu Ala Leu Tyr Asn Thr Ile Asn Glu Ile 
385                 390                 395                 400 


Ala Tyr Asp Asn Leu Lys Glu Lys Gly Glu Asn Ile Leu Pro Tyr Leu 
                405                 410                 415     


Thr Lys Ala Trp Ala Asp Leu Cys Asn Ala Phe Leu Gln Glu Ala Lys 
            420                 425                 430         


Trp Leu Tyr Asn Lys Ser Thr Pro Thr Phe Asp Asp Tyr Phe Gly Asn 
        435                 440                 445             


Ala Trp Lys Ser Ser Ser Gly Pro Leu Gln Leu Val Phe Ala Tyr Phe 
    450                 455                 460                 


Ala Val Val Gln Asn Ile Lys Lys Glu Glu Ile Glu Asn Leu Lys Lys 
465                 470                 475                 480 


Tyr His Asp Ile Ile Ser Arg Pro Ser His Ile Phe Arg Leu Cys Asn 
                485                 490                 495     


Asp Leu Ala Ser Ala Ser Ala Glu Ile Ala Arg Gly Glu Thr Ala Asn 
            500                 505                 510         


Ser Val Ser Cys Tyr Met Arg Thr Lys Gly Ile Ser Glu Glu Leu Ala 
        515                 520                 525             


Thr Glu Ser Val Met Asn Leu Ile Asp Glu Thr Trp Lys Lys Met Asn 
    530                 535                 540                 


Lys Glu Lys Leu Gly Gly Ser Leu Phe Ala Lys Pro Phe Val Glu Thr 
545                 550                 555                 560 


Ala Ile Asn Leu Ala Arg Gln Ser His Cys Thr Tyr His Asn Gly Asp 
                565                 570                 575     


Ala His Thr Ser Pro Asp Glu Leu Thr Arg Lys Arg Val Leu Ser Val 
            580                 585                 590         


Ile Thr Glu Pro Ile Leu Pro Phe Glu Arg 
        595                 600         


<210>  5
<211>  608
<212>  PRT
<213>  Pueraria lobata var. montanta

<400>  5

Met Ala Thr Asn Leu Leu Cys Leu Ser Asn Lys Leu Ser Ser Pro Thr 
1               5                   10                  15      


Pro Thr Pro Ser Thr Arg Phe Pro Gln Ser Lys Asn Phe Ile Thr Gln 
            20                  25                  30          


Lys Thr Ser Leu Ala Asn Pro Lys Pro Trp Arg Val Ile Cys Ala Thr 
        35                  40                  45              


Ser Ser Gln Phe Thr Gln Ile Thr Glu His Asn Ser Arg Arg Ser Ala 
    50                  55                  60                  


Asn Tyr Gln Pro Asn Leu Trp Asn Phe Glu Phe Leu Gln Ser Leu Glu 
65                  70                  75                  80  


Asn Asp Leu Lys Val Glu Lys Leu Glu Glu Lys Ala Thr Lys Leu Glu 
                85                  90                  95      


Glu Glu Val Arg Cys Met Ile Asn Arg Val Asp Thr Gln Pro Leu Ser 
            100                 105                 110         


Leu Leu Glu Leu Ile Asp Asp Val Gln Arg Leu Gly Leu Thr Tyr Lys 
        115                 120                 125             


Phe Glu Lys Asp Ile Ile Lys Ala Leu Glu Asn Ile Val Leu Leu Asp 
    130                 135                 140                 


Glu Asn Lys Lys Asn Lys Ser Asp Leu His Ala Thr Ala Leu Ser Phe 
145                 150                 155                 160 


Arg Leu Leu Arg Gln His Gly Phe Glu Val Ser Gln Asp Val Phe Glu 
                165                 170                 175     


Arg Phe Lys Asp Lys Glu Gly Gly Phe Ser Gly Glu Leu Lys Gly Asp 
            180                 185                 190         


Val Gln Gly Leu Leu Ser Leu Tyr Glu Ala Ser Tyr Leu Gly Phe Glu 
        195                 200                 205             


Gly Glu Asn Leu Leu Glu Glu Ala Arg Thr Phe Ser Ile Thr His Leu 
    210                 215                 220                 


Lys Asn Asn Leu Lys Glu Gly Ile Asn Thr Lys Val Ala Glu Gln Val 
225                 230                 235                 240 


Ser His Ala Leu Glu Leu Pro Tyr His Gln Arg Leu His Arg Leu Glu 
                245                 250                 255     


Ala Arg Trp Phe Leu Asp Lys Tyr Glu Pro Lys Glu Pro His His Gln 
            260                 265                 270         


Leu Leu Leu Glu Leu Ala Lys Leu Asp Phe Asn Met Val Gln Thr Leu 
        275                 280                 285             


His Gln Lys Glu Leu Gln Asp Leu Ser Arg Trp Trp Thr Glu Met Gly 
    290                 295                 300                 


Leu Ala Ser Lys Leu Asp Phe Val Arg Asp Arg Leu Met Glu Val Tyr 
305                 310                 315                 320 


Phe Trp Ala Leu Gly Met Ala Pro Asp Pro Gln Phe Gly Glu Cys Arg 
                325                 330                 335     


Lys Ala Val Thr Lys Met Phe Gly Leu Val Thr Ile Ile Asp Asp Val 
            340                 345                 350         


Tyr Asp Val Tyr Gly Thr Leu Asp Glu Leu Gln Leu Phe Thr Asp Ala 
        355                 360                 365             


Val Glu Arg Trp Asp Val Asn Ala Ile Asn Thr Leu Pro Asp Tyr Met 
    370                 375                 380                 


Lys Leu Cys Phe Leu Ala Leu Tyr Asn Thr Val Asn Asp Thr Ser Tyr 
385                 390                 395                 400 


Ser Ile Leu Lys Glu Lys Gly His Asn Asn Leu Ser Tyr Leu Thr Lys 
                405                 410                 415     


Ser Trp Arg Glu Leu Cys Lys Ala Phe Leu Gln Glu Ala Lys Trp Ser 
            420                 425                 430         


Asn Asn Lys Ile Ile Pro Ala Phe Ser Lys Tyr Leu Glu Asn Ala Ser 
        435                 440                 445             


Val Ser Ser Ser Gly Val Ala Leu Leu Ala Pro Ser Tyr Phe Ser Val 
    450                 455                 460                 


Cys Gln Gln Gln Glu Asp Ile Ser Asp His Ala Leu Arg Ser Leu Thr 
465                 470                 475                 480 


Asp Phe His Gly Leu Val Arg Ser Ser Cys Val Ile Phe Arg Leu Cys 
                485                 490                 495     


Asn Asp Leu Ala Thr Ser Ala Ala Glu Leu Glu Arg Gly Glu Thr Thr 
            500                 505                 510         


Asn Ser Ile Ile Ser Tyr Met His Glu Asn Asp Gly Thr Ser Glu Glu 
        515                 520                 525             


Gln Ala Arg Glu Glu Leu Arg Lys Leu Ile Asp Ala Glu Trp Lys Lys 
    530                 535                 540                 


Met Asn Arg Glu Arg Val Ser Asp Ser Thr Leu Leu Pro Lys Ala Phe 
545                 550                 555                 560 


Met Glu Ile Ala Val Asn Met Ala Arg Val Ser His Cys Thr Tyr Gln 
                565                 570                 575     


Tyr Gly Asp Gly Leu Gly Arg Pro Asp Tyr Ala Thr Glu Asn Arg Ile 
            580                 585                 590         


Lys Leu Leu Leu Ile Asp Pro Phe Pro Ile Asn Gln Leu Met Tyr Val 
        595                 600                 605             


<210>  6
<211>  582
<212>  PRT
<213>  Eucalyptus globulus

<400>  6

Met Ala Leu Arg Leu Leu Phe Thr Pro His Leu Pro Val Leu Ser Ser 
1               5                   10                  15      


Arg Arg Ala Asn Gly Arg Val Arg Cys Ser Ala Ser Thr Gln Ile Ser 
            20                  25                  30          


Asp Pro Gln Glu Gly Arg Arg Ser Ala Asn Tyr Gln Pro Ser Val Trp 
        35                  40                  45              


Thr Tyr Asn Tyr Leu Gln Ser Ile Val Ala Gly Glu Gly Arg Gln Ser 
    50                  55                  60                  


Arg Arg Glu Val Glu Gln Gln Lys Glu Lys Val Gln Ile Leu Glu Glu 
65                  70                  75                  80  


Glu Val Arg Gly Ala Leu Asn Asp Glu Lys Ala Glu Thr Phe Thr Ile 
                85                  90                  95      


Phe Ala Thr Val Asp Asp Ile Gln Arg Leu Gly Leu Gly Asp His Phe 
            100                 105                 110         


Glu Glu Asp Ile Ser Asn Ala Leu Arg Arg Cys Val Ser Lys Gly Ala 
        115                 120                 125             


Val Phe Met Ser Leu Gln Lys Ser Leu His Gly Thr Ala Leu Gly Phe 
    130                 135                 140                 


Arg Leu Leu Arg Gln His Gly Tyr Glu Val Ser Gln Asp Val Phe Lys 
145                 150                 155                 160 


Ile Phe Leu Asp Glu Ser Gly Ser Phe Val Lys Thr Leu Gly Gly Asp 
                165                 170                 175     


Val Gln Gly Val Leu Ser Leu Tyr Glu Ala Ser His Leu Ala Phe Glu 
            180                 185                 190         


Glu Glu Asp Ile Leu His Lys Ala Arg Ser Phe Ala Ile Lys His Leu 
        195                 200                 205             


Glu Asn Leu Asn Ser Asp Val Asp Lys Asp Leu Gln Asp Gln Val Lys 
    210                 215                 220                 


His Glu Leu Glu Leu Pro Leu His Arg Arg Met Pro Leu Leu Glu Ala 
225                 230                 235                 240 


Arg Arg Ser Ile Glu Ala Tyr Ser Arg Arg Glu Tyr Thr Asn Pro Gln 
                245                 250                 255     


Ile Leu Glu Leu Ala Leu Thr Asp Phe Asn Val Ser Gln Ser Thr Leu 
            260                 265                 270         


Gln Arg Asp Leu Gln Glu Met Leu Gly Trp Trp Asn Asn Thr Gly Leu 
        275                 280                 285             


Ala Lys Arg Leu Ser Phe Ala Arg Asp Arg Leu Ile Glu Cys Phe Phe 
    290                 295                 300                 


Trp Ala Val Gly Ile Ala His Glu Pro Ser Leu Ser Ile Cys Arg Lys 
305                 310                 315                 320 


Ala Val Thr Lys Ala Phe Ala Leu Ile Leu Val Leu Asp Asp Val Tyr 
                325                 330                 335     


Asp Val Phe Gly Thr Leu Glu Glu Leu Glu Leu Phe Thr Glu Ala Val 
            340                 345                 350         


Arg Arg Trp Asp Leu Asn Ala Val Glu Asp Leu Pro Val Tyr Met Lys 
        355                 360                 365             


Leu Cys Tyr Leu Ala Leu Tyr Asn Ser Val Asn Glu Met Ala Tyr Glu 
    370                 375                 380                 


Thr Leu Lys Glu Lys Gly Glu Asn Val Ile Pro Tyr Leu Ala Lys Ala 
385                 390                 395                 400 


Trp Tyr Asp Leu Cys Lys Ala Phe Leu Gln Glu Ala Lys Trp Ser Asn 
                405                 410                 415     


Ser Arg Ile Ile Pro Gly Val Glu Glu Tyr Leu Asn Asn Gly Trp Val 
            420                 425                 430         


Ser Ser Ser Gly Ser Val Met Leu Ile His Ala Tyr Phe Leu Ala Ser 
        435                 440                 445             


Pro Ser Ile Arg Lys Glu Glu Leu Glu Ser Leu Glu His Tyr His Asp 
    450                 455                 460                 


Leu Leu Arg Leu Pro Ser Leu Ile Phe Arg Leu Thr Asn Asp Ile Ala 
465                 470                 475                 480 


Ser Ser Ser Ala Glu Leu Glu Arg Gly Glu Thr Thr Asn Ser Ile Arg 
                485                 490                 495     


Cys Phe Met Gln Glu Lys Gly Ile Ser Glu Leu Glu Ala Arg Glu Cys 
            500                 505                 510         


Val Lys Glu Glu Ile Asp Thr Ala Trp Lys Lys Met Asn Lys Tyr Met 
        515                 520                 525             


Val Asp Arg Ser Thr Phe Asn Gln Ser Phe Val Arg Met Thr Tyr Asn 
    530                 535                 540                 


Leu Ala Arg Met Ala His Cys Val Tyr Gln Asp Gly Asp Ala Ile Gly 
545                 550                 555                 560 


Ser Pro Asp Asp Leu Ser Trp Asn Arg Val His Ser Leu Ile Ile Lys 
                565                 570                 575     


Pro Ile Ser Pro Ala Ala 
            580         


<210>  7
<211>  583
<212>  PRT
<213>  Melaleuca alternifolia

<400>  7

Met Ala Leu Arg Leu Leu Ser Thr Pro His Leu Pro Gln Leu Cys Ser 
1               5                   10                  15      


Arg Arg Val Ser Gly Arg Val His Cys Ser Ala Ser Thr Gln Val Ser 
            20                  25                  30          


Asp Ala Gln Gly Gly Arg Arg Ser Ala Asn Tyr Gln Pro Ser Val Trp 
        35                  40                  45              


Thr Tyr Asn Tyr Leu Gln Ser Leu Val Ala Asp Asp Ile Arg Arg Ser 
    50                  55                  60                  


Arg Arg Glu Val Glu Gln Glu Arg Glu Lys Ala Gln Ile Leu Glu Glu 
65                  70                  75                  80  


Asp Val Arg Gly Ala Leu Asn Asp Gly Asn Ala Glu Pro Met Ala Ile 
                85                  90                  95      


Phe Ala Leu Val Asp Asp Ile Gln Arg Leu Gly Leu Gly Arg Tyr Phe 
            100                 105                 110         


Glu Glu Asp Ile Ser Lys Ala Leu Arg Arg Cys Leu Ser Gln Tyr Ala 
        115                 120                 125             


Val Thr Gly Ser Leu Gln Lys Ser Leu His Gly Thr Ala Leu Ser Phe 
    130                 135                 140                 


Arg Val Leu Arg Gln His Gly Phe Glu Val Ser Gln Asp Val Phe Lys 
145                 150                 155                 160 


Ile Phe Met Asp Glu Ser Gly Ser Phe Met Lys Thr Leu Gly Gly Asp 
                165                 170                 175     


Val Gln Gly Met Leu Ser Leu Tyr Glu Ala Ser His Leu Ala Phe Glu 
            180                 185                 190         


Glu Glu Asp Ile Leu His Lys Ala Lys Thr Phe Ala Ile Lys His Leu 
        195                 200                 205             


Glu Asn Leu Asn His Asp Ile Asp Gln Asp Leu Gln Asp His Val Asn 
    210                 215                 220                 


His Glu Leu Glu Leu Pro Leu His Arg Arg Met Pro Leu Leu Glu Ala 
225                 230                 235                 240 


Arg Arg Phe Ile Glu Ala Tyr Ser Arg Arg Ser Asn Val Asn Pro Arg 
                245                 250                 255     


Ile Leu Glu Leu Ala Val Met Lys Phe Asn Ser Ser Gln Leu Thr Leu 
            260                 265                 270         


Gln Arg Asp Leu Gln Asp Met Leu Gly Trp Trp Asn Asn Val Gly Leu 
        275                 280                 285             


Ala Lys Arg Leu Ser Phe Ala Arg Asp Arg Leu Met Glu Cys Phe Phe 
    290                 295                 300                 


Trp Ala Val Gly Ile Ala Arg Glu Pro Ala Leu Ser Asn Cys Arg Lys 
305                 310                 315                 320 


Gly Val Thr Lys Ala Phe Ser Leu Ile Leu Val Leu Asp Asp Val Tyr 
                325                 330                 335     


Asp Val Phe Gly Thr Leu Asp Glu Leu Glu Leu Phe Thr Asp Ala Val 
            340                 345                 350         


Arg Arg Trp His Glu Asp Ala Val Glu Asn Leu Pro Gly Tyr Met Lys 
        355                 360                 365             


Leu Cys Phe Leu Ala Leu Tyr Asn Ser Val Asn Asp Met Ala Tyr Glu 
    370                 375                 380                 


Thr Leu Lys Glu Thr Gly Glu Asn Val Thr Pro Tyr Leu Thr Lys Val 
385                 390                 395                 400 


Trp Tyr Asp Leu Cys Lys Ala Phe Leu Gln Glu Ala Lys Trp Ser Tyr 
                405                 410                 415     


Asn Lys Ile Thr Pro Gly Val Glu Glu Tyr Leu Asn Asn Gly Trp Val 
            420                 425                 430         


Ser Ser Ser Gly Gln Val Met Leu Thr His Ala Tyr Phe Leu Ser Ser 
        435                 440                 445             


Pro Ser Leu Arg Lys Glu Glu Leu Glu Ser Leu Glu His Tyr His Asp 
    450                 455                 460                 


Leu Leu Arg Leu Pro Ser Leu Ile Phe Arg Leu Thr Asn Asp Leu Ala 
465                 470                 475                 480 


Thr Ser Ser Ala Glu Leu Gly Arg Gly Glu Thr Thr Asn Ser Ile Leu 
                485                 490                 495     


Cys Tyr Met Arg Glu Lys Gly Phe Ser Glu Ser Glu Ala Arg Lys Gln 
            500                 505                 510         


Val Ile Glu Gln Ile Asp Thr Ala Trp Arg Gln Met Asn Lys Tyr Met 
        515                 520                 525             


Val Asp His Ser Thr Phe Asn Arg Ser Phe Met Gln Met Thr Tyr Asn 
    530                 535                 540                 


Leu Ala Arg Met Ala His Cys Val Tyr Gln Asp Gly Asp Ala Ile Gly 
545                 550                 555                 560 


Ala Pro Asp Asp Gln Ser Trp Asn Arg Val His Ser Leu Ile Ile Lys 
                565                 570                 575     


Pro Val Ser Leu Ala Pro Cys 
            580             


<210>  8
<211>  576
<212>  PRT
<213>  Malus domestica

<400>  8

Met Glu Phe Arg Val His Leu Gln Ala Asp Asn Glu Gln Lys Ile Phe 
1               5                   10                  15      


Gln Asn Gln Met Lys Pro Glu Pro Glu Ala Ser Tyr Leu Ile Asn Gln 
            20                  25                  30          


Arg Arg Ser Ala Asn Tyr Lys Pro Asn Ile Trp Lys Asn Asp Phe Leu 
        35                  40                  45              


Asp Gln Ser Leu Ile Ser Lys Tyr Asp Gly Asp Glu Tyr Arg Lys Leu 
    50                  55                  60                  


Ser Glu Lys Leu Ile Glu Glu Val Lys Ile Tyr Ile Ser Ala Glu Thr 
65                  70                  75                  80  


Met Asp Leu Val Ala Lys Leu Glu Leu Ile Asp Ser Val Arg Lys Leu 
                85                  90                  95      


Gly Leu Ala Asn Leu Phe Glu Lys Glu Ile Lys Glu Ala Leu Asp Ser 
            100                 105                 110         


Ile Ala Ala Ile Glu Ser Asp Asn Leu Gly Thr Arg Asp Asp Leu Tyr 
        115                 120                 125             


Gly Thr Ala Leu His Phe Lys Ile Leu Arg Gln His Gly Tyr Lys Val 
    130                 135                 140                 


Ser Gln Asp Ile Phe Gly Arg Phe Met Asp Glu Lys Gly Thr Leu Glu 
145                 150                 155                 160 


Asn His His Phe Ala His Leu Lys Gly Met Leu Glu Leu Phe Glu Ala 
                165                 170                 175     


Ser Asn Leu Gly Phe Glu Gly Glu Asp Ile Leu Asp Glu Ala Lys Ala 
            180                 185                 190         


Ser Leu Thr Leu Ala Leu Arg Asp Ser Gly His Ile Cys Tyr Pro Asp 
        195                 200                 205             


Ser Asn Leu Ser Arg Asp Val Val His Ser Leu Glu Leu Pro Ser His 
    210                 215                 220                 


Arg Arg Val Gln Trp Phe Asp Val Lys Trp Gln Ile Asn Ala Tyr Glu 
225                 230                 235                 240 


Lys Asp Ile Cys Arg Val Asn Ala Thr Leu Leu Glu Leu Ala Lys Leu 
                245                 250                 255     


Asn Phe Asn Val Val Gln Ala Gln Leu Gln Lys Asn Leu Arg Glu Ala 
            260                 265                 270         


Ser Arg Trp Trp Ala Asn Leu Gly Ile Ala Asp Asn Leu Lys Phe Ala 
        275                 280                 285             


Arg Asp Arg Leu Val Glu Cys Phe Ala Cys Ala Val Gly Val Ala Phe 
    290                 295                 300                 


Glu Pro Glu His Ser Ser Phe Arg Ile Cys Leu Thr Lys Val Ile Asn 
305                 310                 315                 320 


Leu Val Leu Ile Ile Asp Asp Val Tyr Asp Ile Tyr Gly Ser Glu Glu 
                325                 330                 335     


Glu Leu Lys His Phe Thr Asn Ala Val Asp Arg Trp Asp Ser Arg Glu 
            340                 345                 350         


Thr Glu Gln Leu Pro Glu Cys Met Lys Met Cys Phe Gln Val Leu Tyr 
        355                 360                 365             


Asn Thr Thr Cys Glu Ile Ala Arg Glu Ile Glu Glu Glu Asn Gly Trp 
    370                 375                 380                 


Asn Gln Val Leu Pro Gln Leu Thr Lys Val Trp Ala Asp Phe Cys Lys 
385                 390                 395                 400 


Ala Leu Leu Val Glu Ala Glu Trp Tyr Asn Lys Ser His Ile Pro Thr 
                405                 410                 415     


Leu Glu Glu Tyr Leu Arg Asn Gly Cys Ile Ser Ser Ser Val Ser Val 
            420                 425                 430         


Leu Leu Val His Ser Phe Phe Ser Ile Thr His Glu Gly Thr Lys Glu 
        435                 440                 445             


Met Ala Asp Phe Leu His Lys Asn Glu Asp Leu Leu Tyr Asn Ile Ser 
    450                 455                 460                 


Leu Ile Val Arg Leu Asn Asn Asp Leu Gly Thr Ser Ala Ala Glu Gln 
465                 470                 475                 480 


Glu Arg Gly Asp Ser Pro Ser Ser Ile Val Cys Tyr Met Arg Glu Val 
                485                 490                 495     


Asn Ala Ser Glu Glu Thr Ala Arg Lys Asn Ile Lys Gly Met Ile Asp 
            500                 505                 510         


Asn Ala Trp Lys Lys Val Asn Gly Lys Cys Phe Thr Thr Asn Gln Val 
        515                 520                 525             


Pro Phe Leu Ser Ser Phe Met Asn Asn Ala Thr Asn Met Ala Arg Val 
    530                 535                 540                 


Ala His Ser Leu Tyr Lys Asp Gly Asp Gly Phe Gly Asp Gln Glu Lys 
545                 550                 555                 560 


Gly Pro Arg Thr His Ile Leu Ser Leu Leu Phe Gln Pro Leu Val Asn 
                565                 570                 575     


<210>  9
<211>  601
<212>  PRT
<213>  Vitis vinifera

<400>  9

Met Ala Leu His Leu Phe Tyr Phe Pro Lys Gln Cys Phe Leu Thr His 
1               5                   10                  15      


Asn Leu Pro Gly His Pro Met Lys Lys Pro Pro Arg Gly Thr Thr Ala 
            20                  25                  30          


Gln Ile Arg Cys Ser Ala Asn Glu Gln Ser Phe Ser Leu Met Thr Glu 
        35                  40                  45              


Ser Arg Arg Ser Ala His Tyr Gln Pro Ala Phe Trp Ser Tyr Asp Phe 
    50                  55                  60                  


Val Glu Ser Leu Lys Lys Arg Glu Glu Ile Cys Asp Gly Ser Val Lys 
65                  70                  75                  80  


Glu Leu Glu Lys Met Tyr Glu Asp Arg Ala Arg Lys Leu Glu Asp Glu 
                85                  90                  95      


Val Lys Trp Met Ile His Glu Lys Ser Ala Glu Pro Leu Thr Leu Leu 
            100                 105                 110         


Glu Phe Ile Asp Asp Ile Gln Arg Leu Gly Leu Gly His Arg Phe Glu 
        115                 120                 125             


Asn Asp Ile Lys Arg Ser Leu Asp Lys Ile Leu Leu Leu Glu Gly Ser 
    130                 135                 140                 


Asn Ala Gly Lys Gly Glu Ser Leu His His Thr Ala Leu Arg Phe Arg 
145                 150                 155                 160 


Ile Leu Lys Gln His Gly Tyr Lys Val Ser Gln Glu Val Phe Glu Gly 
                165                 170                 175     


Phe Thr Asp Gln Asn Gly His Phe Lys Ala Cys Leu Cys Lys Asp Val 
            180                 185                 190         


Lys Gly Met Leu Ser Leu Tyr Glu Ala Ser Tyr Leu Ala Ser Glu Gly 
        195                 200                 205             


Glu Thr Leu Leu His Glu Ala Met Ala Phe Leu Lys Met His Leu Lys 
    210                 215                 220                 


Asp Leu Glu Gly Thr Leu Asp Lys Ser Leu Glu Glu Leu Val Asn His 
225                 230                 235                 240 


Ala Met Glu Leu Pro Leu His Arg Arg Met Pro Arg Leu Glu Ala Arg 
                245                 250                 255     


Trp Phe Ile Glu Ala Tyr Lys Arg Arg Glu Gly Ala Asp Asp Val Leu 
            260                 265                 270         


Leu Glu Leu Ala Ile Leu Asp Phe Asn Met Val Gln Trp Thr Leu Gln 
        275                 280                 285             


Asp Asp Leu Gln Asp Met Ser Arg Trp Trp Lys Asp Met Gly Leu Ala 
    290                 295                 300                 


Ser Lys Leu His Phe Ala Arg Asp Arg Leu Met Glu Cys Phe Phe Trp 
305                 310                 315                 320 


Thr Val Gly Met Ala Phe Glu Pro Glu Phe Ser Asn Cys Arg Lys Gly 
                325                 330                 335     


Leu Thr Lys Val Thr Ser Phe Ile Thr Thr Ile Asp Asp Val Tyr Asp 
            340                 345                 350         


Val Tyr Gly Ser Val Asp Glu Leu Glu Leu Phe Thr Asp Ala Val Ala 
        355                 360                 365             


Arg Trp Asp Ile Asn Met Val Asn Asn Leu Pro Gly Tyr Met Lys Leu 
    370                 375                 380                 


Cys Phe Leu Ala Leu Tyr Asn Thr Val Asn Glu Met Ala Tyr Asp Thr 
385                 390                 395                 400 


Leu Lys Glu Gln Gly His Asn Ile Leu Pro Tyr Leu Thr Lys Ala Trp 
                405                 410                 415     


Ala Asp Leu Cys Lys Val Phe Leu Val Glu Ala Lys Trp Cys His Lys 
            420                 425                 430         


Glu Tyr Thr Pro Thr Phe Glu Glu Tyr Leu Glu Asn Gly Trp Arg Ser 
        435                 440                 445             


Val Ser Gly Ala Ala Ile Leu Ile His Ala Tyr Phe Leu Met Ser Lys 
    450                 455                 460                 


Asn Ile Thr Lys Glu Ala Leu Glu Cys Leu Glu Asn Asp His Glu Leu 
465                 470                 475                 480 


Leu Arg Trp Pro Ser Thr Ile Phe Arg Leu Cys Asn Asp Leu Ala Thr 
                485                 490                 495     


Ser Lys Ala Glu Leu Glu Arg Gly Glu Ser Ala Asn Ser Ile Ser Cys 
            500                 505                 510         


Tyr Met His Gln Thr Gly Val Ser Glu Glu Asp Ala Arg Glu His Met 
        515                 520                 525             


Lys Ile Leu Ile Asp Glu Ser Trp Lys Lys Met Asn Lys Val Arg Glu 
    530                 535                 540                 


Met Asp Ser Asp Ser Pro Phe Ala Lys Pro Phe Val Glu Thr Ala Ile 
545                 550                 555                 560 


Asn Leu Ala Arg Ile Ala Gln Cys Thr Tyr Gln Tyr Gly Asp Ser His 
                565                 570                 575     


Gly Ala Pro Asp Ala Arg Ser Lys Lys Arg Val Leu Ser Leu Ile Val 
            580                 585                 590         


Glu Pro Ile Pro Met Asn Leu Lys Lys 
        595                 600     


