                               SEQUENCE LISTING

<110> ADURO BIOTECH, INC.
 
<120> FACULTATIVELY ATTENUATED BACTERIAL SPECIES AND METHODS OF 
      PREPARATION AND USE THEREOF

<130> ANZ-7000-PCT

<140> PCT/US2013/068800
<141> 2013-11-06

<150> 61/723,234
<151> 2012-11-06

<160> 57    

<170> PatentIn version 3.5

<210> 1
<211> 237
<212> PRT
<213> Listeria monocytogenes

<400> 1
Met Asn Ala Gln Ala Glu Glu Phe Lys Lys Tyr Leu Glu Thr Asn Gly 
1               5                   10                  15      


Ile Lys Pro Lys Gln Phe His Lys Lys Glu Leu Ile Phe Asn Gln Trp 
            20                  25                  30          


Asp Pro Gln Glu Tyr Cys Ile Phe Leu Tyr Asp Gly Ile Thr Lys Leu 
        35                  40                  45              


Thr Ser Ile Ser Glu Asn Gly Thr Ile Met Asn Leu Gln Tyr Tyr Lys 
    50                  55                  60                  


Gly Ala Phe Val Ile Met Ser Gly Phe Ile Asp Thr Glu Thr Ser Val 
65                  70                  75                  80  


Gly Tyr Tyr Asn Leu Glu Val Ile Ser Glu Gln Ala Thr Ala Tyr Val 
                85                  90                  95      


Ile Lys Ile Asn Glu Leu Lys Glu Leu Leu Ser Lys Asn Leu Thr His 
            100                 105                 110         


Phe Phe Tyr Val Phe Gln Thr Leu Gln Lys Gln Val Ser Tyr Ser Leu 
        115                 120                 125             


Ala Lys Phe Asn Asp Phe Ser Ile Asn Gly Lys Leu Gly Ser Ile Cys 
    130                 135                 140                 


Gly Gln Leu Leu Ile Leu Thr Tyr Val Tyr Gly Lys Glu Thr Pro Asp 
145                 150                 155                 160 


Gly Ile Lys Ile Thr Leu Asp Asn Leu Thr Met Gln Glu Leu Gly Tyr 
                165                 170                 175     


Ser Ser Gly Ile Ala His Ser Ser Ala Val Ser Arg Ile Ile Ser Lys 
            180                 185                 190         


Leu Lys Gln Glu Lys Val Ile Val Tyr Lys Asn Ser Cys Phe Tyr Val 
        195                 200                 205             


Gln Asn Leu Asp Tyr Leu Lys Arg Tyr Ala Pro Lys Leu Asp Glu Trp 
    210                 215                 220                 


Phe Tyr Leu Ala Cys Pro Ala Thr Trp Gly Lys Leu Asn 
225                 230                 235         


<210> 2
<211> 29
<212> PRT
<213> Listeria monocytogenes

<400> 2
Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala 
            20                  25                  


<210> 3
<211> 100
<212> PRT
<213> Listeria monocytogenes

<400> 3
Val Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys 
                85                  90                  95      


Ala Glu Lys Gly 
            100 


<210> 4
<211> 100
<212> PRT
<213> Listeria monocytogenes

<400> 4
Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys 
                85                  90                  95      


Ala Glu Lys Gly 
            100 


<210> 5
<211> 102
<212> PRT
<213> Listeria monocytogenes

<400> 5
Val Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys 
                85                  90                  95      


Ala Glu Lys Gly Gly Ser 
            100         


<210> 6
<211> 102
<212> PRT
<213> Listeria monocytogenes

<400> 6
Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys 
                85                  90                  95      


Ala Glu Lys Gly Gly Ser 
            100         


<210> 7
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
tttcggccga tgagtaacct attaactgtt cat                                    33


<210> 8
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
tttggatcct tagtctccat cttctaataa t                                      31


<210> 9
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
tttggtaccg gtcatgatga cattaataca aca                                    33


<210> 10
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
tttgagctct atcctaaatg gctttatatc agt                                    33


<210> 11
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
tttaagcttt tggaaattcg attaccccac t                                      31


<210> 12
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
tttggtacct ggttattttc gtcgaataac tgcc                                   34


<210> 13
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
tttggtacct ttgagctctt ttagtaaaaa aacgccagag aagc                        44


<210> 14
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
tttgaattct ccgttgttgc aatattcgct                                        30


<210> 15
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
tttaagcttt ccctgaagaa gaagtagcaa tta                                    33


<210> 16
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
tttggtacca gcttgatttt attcttctat gtcgc                                  35


<210> 17
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
tttggtacct ttgagctcgg aaatgactct aatttgcgaa t                           41


<210> 18
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
tttgaattct ccatgtatac ccaatcgttt agga                                   34


<210> 19
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
ataacttcgt atagcataca ttatacgaac ggtaggaaat gactctaatt tgcgaat          57


<210> 20
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
tttgaattct ccatgtatac ccaatcgttt agga                                   34


<210> 21
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
tttaagcttt ccctgaagaa gaagtagcaa tta                                    33


<210> 22
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
taccgttcgt ataatgtatg ctatacgaag ttatagcttg attttattct tctatgtcgc       60


<210> 23
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
tttgaattca cagaaggaga ttgtgaaatg                                        30


<210> 24
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 24
gggtctagat ttggtaccaa tagaagcgta ctgcgact                               38


<210> 25
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 25
gggtctagag tttcacgtga aacattcta                                         29


<210> 26
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 26
tttaagcttc ggaattggtt caagactgg                                         29


<210> 27
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 27
attggtacct tcgaggagta aacttcccaa                                        30


<210> 28
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 28
aactctagac accgcggtgg cggccgataa                                        30


<210> 29
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 29
tatgcggccg cgggaagcag ttggggttaa ct                                     32


<210> 30
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 30
aactctagac ttagtctcca tcttctaata                                        30


<210> 31
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 31
aaaggaagtt cctattctct agaaagtata ggaacttctg cggaaatgac tctaatttgc       60


<210> 32
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 32
gcagaagttc ctatactttc tagagaatag gaacttcctt tagcttgatt ttattcttct       60


<210> 33
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 33
aaaggaagtt cctattctct agaaagtata ggaacttctg cttttagtaa aaaaacgcca       60


<210> 34
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 34
gcagaagttc ctatactttc tagagaatag gaacttcctt ttggttattt tcgtcgaata       60


<210> 35
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 35
tttctgcagg tggatagaac tcataaagga c                                      31


<210> 36
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
tttggtacct cagttaaccc caactgcttc                                        30


<210> 37
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
tttggtacct ttagatctaa acacagaacg aaagaaaaag                             40


<210> 38
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 38
tttgaattcc cagtaggttc cactgtatc                                         29


<210> 39
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
tttctgcagt ccatgtatac ccaatcgttt agga                                   34


<210> 40
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 40
tttaagcttt ccctgaagaa gaagtagcaa tta                                    33


<210> 41
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 41
tttagatcta aatggttttt ctctctataa                                        30


<210> 42
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 42
tagatctata acttcgtata gcatacatta tacgaacggt attcgaaaat tattgcgtta       60


<210> 43
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 43
tttctgcaga tgattcaatc cttcttgctt                                        30


<210> 44
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 44
gggtctagag agttatacaa aacgggaata                                        30


<210> 45
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 45
ataacttcgt atagcataca ttatacgaac ggta                                   34


<210> 46
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 46
taccgttcgt atagcataca ttatacgaag ttat                                   34


<210> 47
<211> 34
<212> DNA
<213> Listeria monocytogenes

<400> 47
ataacttcgt ataatgtatg ctatacgaag ttat                                   34


<210> 48
<211> 221
<212> DNA
<213> Listeria monocytogenes

<400> 48
gggaagcagt tggggttaac tgattaacaa atgttagaga aaaattaatt ctccaagtga       60

tattcttaaa ataattcatg aatatttttt cttatattag ctaattaaga agataattaa      120

ctgctaatcc aatttttaac ggaataaatt agtgaaaatg aaggccgaat tttccttgtt      180

ctaaaaaggt tgtattagcg tatcacgagg agggagtata a                          221


<210> 49
<211> 1032
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 49
atgagtaacc tattaactgt tcatcaaaat ttaccagcat taccagtgga tgcaacatca       60

gatgaagtaa gaaaaaattt aatggatatg tttagagacc gacaagcctt ttcggagcat      120

acatggaaaa tgttattatc tgtttgtaga tcatgggcag catggtgcaa acttaacaat      180

agaaaatggt ttccagcaga accagaagat gtacgagatt atttattata ccttcaagca      240

agaggattag cagtaaaaac cattcaacaa catttaggac aattaaatat gttacataga      300

cgatcaggat taccaagacc tagcgattct aacgcagtta gtttagttat gagaagaatt      360

agaaaagaaa atgtcgatgc aggcgaacga gcaaaacaag cactagcatt tgaacgtaca      420

gatttcgacc aagtaagatc attaatggaa aatagcgacc gttgtcaaga catccgaaac      480

ttagcttttt taggaatagc atacaacaca ttattaagaa tagcagaaat agccagaatt      540

agagtaaaag acattagtag aacagatgga ggaagaatgt taattcatat tggaagaaca      600

aaaacattag tatcaacagc cggggtagaa aaagcgttat cattaggagt tacaaaatta      660

gtagaacgat ggatttcagt ttcaggagtg gcagatgacc caaataatta tttattttgt      720

agagtacgaa aaaacggagt agcagcacct tcagcaacaa gtcaattaag tacaagagca      780

ttagaaggaa tattcgaagc aacacatcga ctaatttacg gagcaaaaga tgatagtgga      840

caacgatatt tagcttggag tggacacagt gcgcgagtag gagcagcaag agatatggca      900

agagcgggag ttagtatacc agaaataatg caagcaggag gatggacaaa tgtaaatatt      960

gtaatgaatt atattagaaa tttagatagt gaaaccggtg caatggtacg attattagaa     1020

gatggagact aa                                                         1032


<210> 50
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 50
gaagttccta ttctctagaa agtataggaa cttc                                   34


<210> 51
<211> 1272
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 51
atgtcgcaat ttgatatact atgtaaaact ccacctaaag tattagtgcg tcaatttgtt       60

gaacgttttg aacgaccaag tggcgaaaag atagcttcct gtgccgcgga acttacttac      120

ttgtgttgga tgattacaca taatggcact gcaatcaaaa gagcaacatt catgtcatac      180

aacaccatca tttctaattc tttatcattt gatattgtta acaaaagttt acaattcaaa      240

tacaaaactc aaaaagcgac gattcttgaa gctagtttga aaaagttaat cccagcatgg      300

gagtttacca tcattcctta caatggacag aaacaccaat ccgacattac agacattgtt      360

tctagtttac aactacaatt tgaaagcagt gaagaagcgg ataaagggaa ctcacattcg      420

aagaaaatgt taaaggcttt gttatctgaa ggagaatcta tctgggagat tacagaaaag      480

attctaaact cttttgagta tacttcacgc tttactaaaa ccaaaacgtt ataccagttt      540

ctttttctag ctacattcat taactgcggt cgatttagtg acattaagaa tgtagatcct      600

aaatcgttca agttagtcca aaacaagtat ctaggtgtca tcattcaatg cttagttacg      660

gaaacaaaaa cgagtgtaag tagacatatc tatttctttt ctgctagagg tagaattgat      720

ccgcttgtat acttagatga atttctacgt aattcagagc cggtgcttaa acgcgttaat      780

cgtacaggaa atagctcaag caataagcaa gaatatcaac ttttgaaaga caatttggtg      840

cgtagctata acaaagcgtt aaagaagaat gcaccatatc cgatattcgc catcaaaaac      900

gggccaaaat cccacattgg tcgccatctt atgactagct tcctttcgat gaaaggatta      960

acggagttaa caaatgtggt aggtaattgg tccgacaaaa gagcgagtgc tgtagcacga     1020

acgacatata cacatcagat tacagctatt ccagatcact actttgcatt agttagtaga     1080

tactatgcat atgatccaat ttccaaagaa atgattgctc ttaaagatga aacaaatcca     1140

atagaagaat ggcaacatat cgaacaactt aaaggatcgg cagaaggctc tatacgttat     1200

cctgcatgga atggtatcat ttctcaagaa gttttagact atttgtcaag ctatatcaat     1260

cgtcgcattt aa                                                         1272


<210> 52
<211> 9
<212> PRT
<213> Vaccinia virus

<400> 52
Tyr Ala Pro Val Ser Pro Ile Val Ile 
1               5                   


<210> 53
<211> 8
<212> PRT
<213> Vaccinia virus

<400> 53
Leu Asn Phe Arg Phe Glu Asn Val 
1               5               


<210> 54
<211> 10
<212> PRT
<213> Vaccinia virus

<400> 54
Tyr Ser Leu Pro Asn Ala Gly Asp Val Ile 
1               5                   10  


<210> 55
<211> 8
<212> PRT
<213> Vaccinia virus

<400> 55
Thr Ser Tyr Lys Phe Glu Ser Val 
1               5               


<210> 56
<211> 8
<212> PRT
<213> Gallus sp.

<400> 56
Ser Ile Ile Asn Phe Glu Lys Leu 
1               5               


<210> 57
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 57
Ser Pro Ser Tyr Ala Tyr His Gln Phe 
1               5                   


