                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  Compositions and Methods for Oxygenation of Nucleic Acids 
       Containing 5-Methylpyrimidine

<130>  NEB-364-B-PCT

<150>  61/722,968
<151>  2012-11-06

<150>  61/723,427
<151>  2012-11-07

<150>  61/724,041
<151>  2012-11-08

<150>  13/827,885
<151>  2013-03-14

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be either Arginine or Lysine

<220>
<221>  MISC_FEATURE
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(20)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (22)..(52)
<223>  There can be 18-31 amino acids at this location. Xaa can be any 
       naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (54)..(63)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (65)..(65)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (67)..(138)
<223>  There can be 7-72 amino acids at this location.  Xaa can be any 
       naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (140)..(144)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Xaa Xaa Xaa Xaa Xaa His Xaa Asp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His 
    50                  55                  60                  


Xaa Val Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            100                 105                 110         


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        115                 120                 125             


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Arg Xaa Xaa Xaa Xaa Xaa 
    130                 135                 140                 


Phe Ala 
145     


<210>  2
<211>  321
<212>  PRT
<213>  Naegleria gruberi

<400>  2

Met Thr Thr Phe Lys Gln Gln Thr Ile Lys Glu Lys Glu Thr Lys Arg 
1               5                   10                  15      


Lys Tyr Cys Ile Lys Gly Thr Thr Ala Asn Leu Thr Gln Thr His Pro 
            20                  25                  30          


Asn Gly Pro Val Cys Val Asn Arg Gly Glu Glu Val Ala Asn Thr Thr 
        35                  40                  45              


Thr Leu Leu Asp Ser Gly Gly Gly Ile Asn Lys Lys Ser Leu Leu Gln 
    50                  55                  60                  


Asn Leu Leu Ser Lys Cys Lys Thr Thr Phe Gln Gln Ser Phe Thr Asn 
65                  70                  75                  80  


Ala Asn Ile Thr Leu Lys Asp Glu Lys Trp Leu Lys Asn Val Arg Thr 
                85                  90                  95      


Ala Tyr Phe Val Cys Asp His Asp Gly Ser Val Glu Leu Ala Tyr Leu 
            100                 105                 110         


Pro Asn Val Leu Pro Lys Glu Leu Val Glu Glu Phe Thr Glu Lys Phe 
        115                 120                 125             


Glu Ser Ile Gln Thr Gly Arg Lys Lys Asp Thr Gly Tyr Ser Gly Ile 
    130                 135                 140                 


Leu Asp Asn Ser Met Pro Phe Asn Tyr Val Thr Ala Asp Leu Ser Gln 
145                 150                 155                 160 


Glu Leu Gly Gln Tyr Leu Ser Glu Ile Val Asn Pro Gln Ile Asn Tyr 
                165                 170                 175     


Tyr Ile Ser Lys Leu Leu Thr Cys Val Ser Ser Arg Thr Ile Asn Tyr 
            180                 185                 190         


Leu Val Ser Leu Asn Asp Ser Tyr Tyr Ala Leu Asn Asn Cys Leu Tyr 
        195                 200                 205             


Pro Ser Thr Ala Phe Asn Ser Leu Lys Pro Ser Asn Asp Gly His Arg 
    210                 215                 220                 


Ile Arg Lys Pro His Lys Asp Asn Leu Asp Ile Thr Pro Ser Ser Leu 
225                 230                 235                 240 


Phe Tyr Phe Gly Asn Phe Gln Asn Thr Glu Gly Tyr Leu Glu Leu Thr 
                245                 250                 255     


Asp Lys Asn Cys Lys Val Phe Val Gln Pro Gly Asp Val Leu Phe Phe 
            260                 265                 270         


Lys Gly Asn Glu Tyr Lys His Val Val Ala Asn Ile Thr Ser Gly Trp 
        275                 280                 285             


Arg Ile Gly Leu Val Tyr Phe Ala His Lys Gly Ser Lys Thr Lys Pro 
    290                 295                 300                 


Tyr Tyr Glu Asp Thr Gln Lys Asn Ser Leu Lys Ile His Lys Glu Thr 
305                 310                 315                 320 


Lys 
    


<210>  3
<211>  174
<212>  PRT
<213>  Naegleria gruberi

<400>  3

Met Pro Met Asn Tyr Ile Thr Ser Asp Leu Lys Thr Gln Leu Gly Glu 
1               5                   10                  15      


Tyr Leu Ile Gly Ile Val Asn Pro Met Leu Asp Glu Thr Ile Thr Ala 
            20                  25                  30          


Ala Leu Glu Ile Leu Ser Pro Arg Thr Ile Asn Tyr Leu Thr Ser Leu 
        35                  40                  45              


Pro His Pro Tyr His Ile Leu Asn Asn Cys Ile Tyr Pro Ser Thr Ala 
    50                  55                  60                  


Phe Asn Tyr Leu Glu Pro Gln Ile Glu Lys His Arg Ile Lys Asn Ala 
65                  70                  75                  80  


His Lys Asp Thr Arg Asp Ala Thr Pro Ser Val Leu Phe Tyr Leu Gly 
                85                  90                  95      


Asp Tyr Asp Glu Lys Glu Gly Tyr Leu Glu Phe Pro Glu Gln Asn Cys 
            100                 105                 110         


Lys Val Phe Val Lys Pro Gly Asp Leu Leu Leu Phe Lys Gly Asn Lys 
        115                 120                 125             


Tyr Lys His Gln Val Ala Pro Ile Thr Ser Gly Thr Arg Leu Gly Leu 
    130                 135                 140                 


Val Tyr Phe Ala His Lys Ala Cys Lys Val Met Asp Phe Tyr Asp Asp 
145                 150                 155                 160 


Tyr Gln Lys Glu Ser Leu Asn Lys His Lys Gln Gln Asn Gln 
                165                 170                 


<210>  4
<211>  583
<212>  PRT
<213>  Naegleria gruberi

<400>  4

Met Ser Ile Asn Thr Thr Phe Asn Gln Lys Thr Thr Gln Ser Gly Glu 
1               5                   10                  15      


Pro Pro Met Met Met Arg Met Thr Asn Ser Ser Thr Pro Pro Leu Thr 
            20                  25                  30          


Pro Lys Asn Cys Leu Pro Ile Phe Val Tyr Asn Asp Tyr Gly Lys Leu 
        35                  40                  45              


Ile Arg Glu Glu Gln Gln Gln Pro Thr Asp Ile Ile Thr Asn Asn Asn 
    50                  55                  60                  


Asn Ser Met Met Arg Ser Met Pro Thr Thr Asn Arg Trp Glu Thr Asn 
65                  70                  75                  80  


Pro Gln Thr Pro Leu Ser Val Ser Pro Phe Gln Pro Leu Leu Pro Ile 
                85                  90                  95      


Pro Asn Phe Ser His Ala Phe Ile Val Gly Asn Leu Pro Pro Ser Val 
            100                 105                 110         


Ser Val Arg Arg Lys Asn Arg Lys Met Ser Glu Lys Pro Lys Asn Asn 
        115                 120                 125             


Ser Ala Pro Ser Lys Ile Met His Gln Leu Glu Leu Ser Val Leu Asn 
    130                 135                 140                 


Asn Gln Arg Arg Ile Ala Pro Lys Gly Pro Leu Ala Asp Ile Ser Asn 
145                 150                 155                 160 


Ile Gln Leu Pro Gln Gln Glu Ser Thr Asn Lys Ser Asn Asn Thr Thr 
                165                 170                 175     


Pro Lys Lys Pro Arg Ile Arg Gln Leu Met Leu Thr Thr Pro Leu Arg 
            180                 185                 190         


Glu Ser Leu Gln Ser Asn Gln Ser Ala Arg Ser Lys Tyr Ile Asp Glu 
        195                 200                 205             


Glu Ala Asn Asn Tyr Ser Ile Asn Asp Ser Pro Glu Thr Thr Ile Ile 
    210                 215                 220                 


Lys Thr Ser Asn Thr Lys Asp Ser Glu His Lys Ala Ala Met Ala Thr 
225                 230                 235                 240 


Asn Leu Gly Leu Ser Thr Asp Asp Phe Glu Cys Lys Pro Phe Glu Thr 
                245                 250                 255     


Thr Thr Leu Pro Ser Val Ile Asp Lys Asn Tyr Leu Val Val Asp Lys 
            260                 265                 270         


Glu Gly Cys Thr Gln Leu Ala Leu Leu Pro Asn His Ile Pro Thr Ser 
        275                 280                 285             


Val Cys Lys Leu Ile Glu Val Lys Cys Arg Lys Val Ser Asn Leu Arg 
    290                 295                 300                 


His Ala Leu Lys Ile Gln Lys Ala Ser Phe Tyr Val Asn Trp Trp Thr 
305                 310                 315                 320 


Lys Ser Gln Pro Met Gly Tyr Met Cys Lys Asp Asn Glu Ser Glu Ile 
                325                 330                 335     


Gly Lys Val Val Asn Glu Ile Ala Glu Leu Leu Ser Asp His Cys Arg 
            340                 345                 350         


Asn Leu Leu Arg Met Cys Asn Glu Arg Val Tyr Lys Lys Ile Ser Glu 
        355                 360                 365             


Leu Lys Glu Asp Lys Phe Phe Ala Pro Cys Ile Cys Phe Asn Ile Leu 
    370                 375                 380                 


Glu His Asp Leu Glu Ser Arg Ile Thr Lys Phe His His Asp Lys Met 
385                 390                 395                 400 


Asp Tyr Gly Val Ser Val Leu Phe Tyr Phe Gly Asp Tyr Ser Arg Gly 
                405                 410                 415     


Asn Leu Asn Val Leu Asp Ala Gly Ser Ser Ser Thr Ile Val Thr Arg 
            420                 425                 430         


Pro Gly Asp Ala Val Ile Leu Arg Gly Asn Tyr Tyr Lys His Ser Val 
        435                 440                 445             


Gln Asn Ile Glu Pro Gly Asn Asn Lys Ala Arg Tyr Ser Ile Val Phe 
    450                 455                 460                 


Phe Ala His Ser Thr His Phe Leu Lys Lys Lys Tyr Glu Leu Ser Pro 
465                 470                 475                 480 


Ala Ala Ala Lys Lys Ala Phe Leu Val Asp Asn Pro Asp Phe Val Ser 
                485                 490                 495     


Ile Lys Lys Arg Lys Gln Ala Ser Ser Ser Ser Asp Val Ser Val Lys 
            500                 505                 510         


Lys Ser Lys Lys Ser Thr Glu Asp Asn Val Glu Phe Ile Gln Thr His 
        515                 520                 525             


Thr Tyr Leu Gly Asn Gly Tyr Lys Ser Gly His Lys Asn Tyr Gln Tyr 
    530                 535                 540                 


Tyr Val Lys Phe Asn Asn Ser Asp Gln Lys Glu Trp Lys Ser Tyr Glu 
545                 550                 555                 560 


Ser Leu Pro Lys Gln Ala Val Ala Ser Tyr Trp Val Lys Phe Lys Lys 
                565                 570                 575     


Leu Lys Ser Leu Ser Asn Gln 
            580             


<210>  5
<211>  207
<212>  PRT
<213>  Naegleria gruberi

<400>  5

Met Leu Glu Ala Gln His His Lys Leu Thr Ile Tyr Thr Gly Met Trp 
1               5                   10                  15      


Gly His Met Lys Pro Cys Val Phe Ile Ala Ala Asp Asn Cys Asn Lys 
            20                  25                  30          


Ser Gly Glu Thr Ile Val Glu Asn Leu Leu Phe Lys Leu Gly Lys Ile 
        35                  40                  45              


Gly Ser Lys Leu Met Glu Ile Leu Ser Pro Phe Thr Met Asn Phe Leu 
    50                  55                  60                  


Ser Ser Leu Asp Pro Glu Ile Phe Leu Asn His Asp Leu Phe Pro Ile 
65                  70                  75                  80  


Ser Ala Thr Asn Phe Met Ile Pro Gly Asn Lys His Arg Ile Leu Lys 
                85                  90                  95      


Pro His Lys Asp Asn Gln Asp Val Gly Leu Cys Ile Ile Phe Tyr Phe 
            100                 105                 110         


Gly Asn Tyr Asn Ala Pro Leu Glu Phe Val Asn Lys Gly Ser Val Phe 
        115                 120                 125             


Asn Thr Glu Arg Gly Asp Val Leu Leu Met Arg Gly Ser His Phe Arg 
    130                 135                 140                 


His Val Val Lys Pro Val Asp Asn Gly Leu Leu Glu His Val His Asp 
145                 150                 155                 160 


Pro Met Arg Ile Ser Val Val Leu Phe Ala His Lys Ser Leu Lys Met 
                165                 170                 175     


Asn Pro Ser Tyr Phe Leu Asn Ala Gly Ser Ala Leu Lys Ala His Asp 
            180                 185                 190         


Glu Asp Phe Pro Glu Lys Ala Lys Lys Arg Lys Lys Lys Arg Lys 
        195                 200                 205         


<210>  6
<211>  211
<212>  PRT
<213>  Naegleria gruberi

<400>  6

Met Phe Leu Arg Asn Ile Leu Pro Glu Asn Thr Thr Thr Glu Val Thr 
1               5                   10                  15      


Asn Ile Leu Asp Lys Ile Asn Gln Arg Arg Ser Lys Glu Asn Tyr Tyr 
            20                  25                  30          


Ile Gly Ser Trp Gly Lys Ser Ser Ser Phe Leu Phe Lys Thr Asn Asp 
        35                  40                  45              


Thr Ile Phe Asn Glu Leu Ser Ser Gln Phe Ile Lys Ile Ile Asn Leu 
    50                  55                  60                  


Leu Lys Asn Tyr Val Leu Glu Ile Leu Lys Phe Gly Asn Asn Lys Met 
65                  70                  75                  80  


Arg Lys Phe Leu Glu Lys Tyr Asn Ser Ser Asp Phe Leu Ser Ile Tyr 
                85                  90                  95      


Pro Thr Val Cys Phe Asn Phe Leu Asp Lys Ser Val Asp Glu Asn Arg 
            100                 105                 110         


Ile Leu His Ile His Pro Asp Lys Glu Asp Thr Gly Thr Ser Leu Ile 
        115                 120                 125             


Phe Tyr Phe Gly Lys Phe Lys Gly Gly Ala Ile Ser Phe Pro Glu Leu 
    130                 135                 140                 


Asn Phe Lys Leu Met Val Gln Ser Ala Asp Val Leu Leu Phe Asp Gly 
145                 150                 155                 160 


Lys Asn Asn Leu His Ala Val Glu Ser Leu His Gly Lys Asp Asp Val 
                165                 170                 175     


Arg Tyr Ser Val Val Phe Phe Ala His Lys Ala Asp Leu Gly Lys Thr 
            180                 185                 190         


Ser Tyr Pro Met Asn Arg Gly Glu Val Met Lys Gly Ile Lys Asn Lys 
        195                 200                 205             


Ile Asn Asn 
    210     


<210>  7
<211>  302
<212>  PRT
<213>  Naegleria gruberi

<400>  7

Met Asp Ile Gly Ile Asp Trp Arg Gly Thr His Phe Arg His Lys Asn 
1               5                   10                  15      


His Leu Val Lys Glu Glu Val Cys Asp Arg Thr Asn Trp Ile Val Leu 
            20                  25                  30          


Cys Pro Asn Gly Gln Val Asp Ile Ala Phe Phe Pro Asn Ala Ile Pro 
        35                  40                  45              


Glu Glu Leu Cys Leu Glu Met Glu Thr Val Val Ala Asn Ser Asp Val 
    50                  55                  60                  


Asp Ile Leu Ser Cys Lys Lys Ala Ile Ile Asp Gly Ser Trp Thr Arg 
65                  70                  75                  80  


Tyr Gly Asn Gly Ile Tyr Pro Val Lys Thr Ile Thr Thr Asn Gln Ser 
                85                  90                  95      


Ile Leu Leu His Glu Leu Asn Asp Lys Cys Gly Pro Phe Val Leu Asp 
            100                 105                 110         


Lys Leu Lys His Ile Asn Lys Asn Met Phe Asn Lys Leu Asp Asn Ile 
        115                 120                 125             


Asn Glu Asp Ile Lys Asn Tyr Lys Ile Phe Ala Lys Tyr Pro Thr Leu 
    130                 135                 140                 


Ala Leu Asn Val Ser His Asn Glu Asn Tyr Asn Ile Ser Lys Lys Pro 
145                 150                 155                 160 


Tyr Arg Lys His Thr Asp Gly Asn Asp Ile Gly Leu Gly Val Leu Thr 
                165                 170                 175     


Tyr Phe Gly Ser Glu Ile Ile Glu Gly Gly Asn Leu Ile Ile His Ile 
            180                 185                 190         


Glu Asn Leu Lys Val Phe Asn Phe Pro Ile Gln Arg Arg Asp Leu Val 
        195                 200                 205             


Phe Leu Asn Ser Lys Phe Tyr Ala His Gln Val Thr Lys Val Thr Ser 
    210                 215                 220                 


Gly Ile Arg Phe Gly Leu Val Tyr Phe Ala Gly Glu Ala His Phe Arg 
225                 230                 235                 240 


Val Arg Asn Asn Asp Asp Phe Leu Pro Ala Leu Pro Phe Asn Ala Asn 
                245                 250                 255     


Asp Lys Glu Leu Arg Glu Glu Arg Ser Lys Lys Gly Arg Lys Ser Met 
            260                 265                 270         


Asn Glu Tyr Lys Lys Arg Phe Leu Lys Lys Tyr Leu Arg Glu Lys Lys 
        275                 280                 285             


Lys Ile Asn Lys Lys Arg Val Lys Cys Lys Asn Lys Leu Lys 
    290                 295                 300         


<210>  8
<211>  575
<212>  PRT
<213>  Naegleria gruberi

<400>  8

Met Gly Pro Leu His Val Ser Gln His Asp Lys Lys Lys Pro Lys His 
1               5                   10                  15      


Arg Arg Arg Lys Lys Gln Phe Leu Lys Ala Gln Ala Leu Thr Arg Val 
            20                  25                  30          


Cys Trp Glu Asn Glu Lys Ser Ile Asp Glu Ser Gly Lys Thr Arg Val 
        35                  40                  45              


Tyr Lys Met Ile Lys Glu Trp Glu Phe Leu Lys Gly Asn Asn Ile Gln 
    50                  55                  60                  


Ser Asn Glu Pro Ile Leu Ser Val Tyr Gly Val Asn Asp Thr Ile Pro 
65                  70                  75                  80  


Lys Glu Ile Ser Ser Asn Thr Ile Ile Val Thr Lys Glu Gly Met Val 
                85                  90                  95      


Glu Met Ala Leu Leu Lys Ser Val Leu Pro Pro Ser Leu Leu Glu Glu 
            100                 105                 110         


Cys Thr Gln Leu Cys Arg Glu Met Ser Glu Trp Leu Ala Thr Glu Lys 
        115                 120                 125             


Asp Ile Asp Lys Gly Ser Phe Phe Ser Gly Trp Trp Thr Met Asn Met 
    130                 135                 140                 


Pro Met Gly Tyr Lys Cys Ala Asp Ser Phe Arg Phe Glu Leu Val Asp 
145                 150                 155                 160 


Thr Lys Val Lys Gln Ile Gln Ala Leu Leu His Asp Thr Phe Gln His 
                165                 170                 175     


Ile Leu Glu Leu Ala Asn Pro Lys Leu Phe Ala Lys Leu Ser Lys Leu 
            180                 185                 190         


Thr Glu Arg Gly Gln Thr Pro Val Val Cys Phe Asn Met Ile Pro Thr 
        195                 200                 205             


Arg Asn Glu Ser Val Lys Glu Lys Phe Gln Gly Ser Tyr Lys Ser Thr 
    210                 215                 220                 


Asp Lys Val Asn Arg Pro Lys Thr Asn His Arg Asp Arg Asn Asp Met 
225                 230                 235                 240 


Gly Ile Ser Ala Met Phe Tyr Met Gly Lys Phe Gly Gly Gly Ser Leu 
                245                 250                 255     


Gln Leu Ile Arg Val Asn Glu His Thr Pro Lys Thr Leu Val His Ile 
            260                 265                 270         


Gln Ala Gly Asp Val Val Leu Leu Arg Ala Asn Lys Tyr Arg His Ala 
        275                 280                 285             


Val Ser Pro Thr Arg Pro Gln Ser Phe Pro Leu Ala Asn Ser Ser Gln 
    290                 295                 300                 


Thr Glu Val Asp Asp Val Lys Ile Cys Glu Asn Ser Ser Pro Thr Leu 
305                 310                 315                 320 


Asn Asn Pro Gln Ala Asp Asp Asn Thr Pro Thr Leu Ile Asn Thr Cys 
                325                 330                 335     


Pro Lys Gln Glu Pro Thr Asp Gly Asp Asn Pro Val Gln Ser Ser Lys 
            340                 345                 350         


Glu Pro Ser Asn Asp Tyr Glu Gln Lys Arg Phe Ser Phe Ile Phe Phe 
        355                 360                 365             


Ala His Arg Ser His Phe Lys His Ser Lys Val Tyr Cys Gly Met Gly 
    370                 375                 380                 


Gln Arg Gln Ala Leu Asn Ala Phe Lys Ala Asp His Pro Tyr Tyr Gln 
385                 390                 395                 400 


Ser Gln Arg Met Lys Lys Lys Leu Gly Asp Asp Cys Leu Asp Gln Ser 
                405                 410                 415     


Leu Ile Leu Thr Glu Lys Arg Lys Pro Ile Lys Arg Asn Tyr Ala Leu 
            420                 425                 430         


Phe Asn Glu Cys Gly Asp Asp Lys Gln Glu Glu Ser Asp Glu Glu Glu 
        435                 440                 445             


Tyr Gln Gln Tyr Glu Pro Lys Pro Thr Thr Glu Glu Tyr Thr Ile Lys 
    450                 455                 460                 


Val Ile Val Asp His Glu Lys Val Phe Lys Gly Ser Asp Gln Ser Arg 
465                 470                 475                 480 


Lys Ser Tyr Leu Tyr His Ile Gln Trp Leu Gly Tyr Pro Asp Glu Thr 
                485                 490                 495     


Trp Glu Pro Tyr Glu His Leu Asp Asp Cys Gln Val Phe Glu Asp Tyr 
            500                 505                 510         


Leu Lys His His Asn Ile Ser Leu Phe Asp Glu Glu Glu Glu Asp Arg 
        515                 520                 525             


Lys Val Asp Asp Ser Met Leu Leu Pro Ala Trp Met His Glu Asp Glu 
    530                 535                 540                 


Ser Leu Phe Glu Ala Leu Leu Pro Ile Ile Cys Cys Ser Thr Asp Asn 
545                 550                 555                 560 


Pro Arg His His Leu Asp Asp Val Pro Pro Phe Asp Phe Asn Tyr 
                565                 570                 575 


<210>  9
<211>  253
<212>  PRT
<213>  Naegleria gruberi

<400>  9

Met Thr Glu Ile Val Glu Leu Ser Asn Ile Glu Pro Lys Asp Gln Lys 
1               5                   10                  15      


Gln Ala Ile Ile Gly Gly Thr Trp Asn Arg Tyr Gly Asn Ser Ile Glu 
            20                  25                  30          


Ile Val Ala Gly Ile Ser Asp Glu Asn Asn Thr Leu Leu Asp Asn Leu 
        35                  40                  45              


Thr Asn Cys Cys Glu Ser Phe Val Leu Asp Lys Leu Trp His Leu Asn 
    50                  55                  60                  


Arg Ser Met Tyr Asn Lys Leu Asp Thr Ile Glu Glu Lys Ile Lys Asn 
65                  70                  75                  80  


Phe Lys Thr Tyr Ala Lys Tyr Pro Ser Leu Ala Leu Asn Leu Leu Cys 
                85                  90                  95      


Lys Glu Asn Tyr Asn Gly Lys Val Lys Pro Tyr Arg Lys His Ile Asp 
            100                 105                 110         


Pro Asn Asn Asn Gly Met Asp Val Leu Met Phe Phe Gly Lys Thr Phe 
        115                 120                 125             


Glu Gly Gly Asn Leu Ile Val Ser Tyr His Tyr Thr Asn Ile Asp Phe 
    130                 135                 140                 


Arg Met Phe Thr Leu Pro Ile Gln Ser Gly Asp Leu Val Phe Leu Asn 
145                 150                 155                 160 


Ser Arg Ile Tyr His His Lys Val Thr Lys Val Thr Ser Gly Val Arg 
                165                 170                 175     


Cys Gly Leu Val Phe Phe Ala Gly Leu Asp His Phe Ser Val Arg Lys 
            180                 185                 190         


Ala Asn Tyr Lys Lys Val Lys Lys Glu Glu Tyr Gln Lys Asn Met Asp 
        195                 200                 205             


Asp Lys Leu Leu Ala Leu Pro Phe Gln Gln Lys Asp Lys Asp Leu Arg 
    210                 215                 220                 


Ile Glu Arg Thr Lys Thr Gly Arg Lys Glu Ile Lys Gln Phe His Lys 
225                 230                 235                 240 


Asn Leu Gln Asn Asn Leu Pro Asn Lys Lys Arg Lys Lys 
                245                 250             


<210>  10
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  10
attacacgcg cgatatcgtt aacgataatt cgcgcgatta cgatcgataa cgcgttaata       60


<210>  11
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  11
cggcgtttcc gggttccata ggctccgccc cggactctga tgaccagggc atcac            55


<210>  12
<211>  1766
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  12
gggtctagaa ataattttgt ttaactttaa gaaggagata tacatatgaa aatcgaagaa       60

ggtaaaggtc accatcacca tcaccacgga tccatggaag acgccaaaaa cataaagaaa      120

ggcccggcgc cattctatcc tctagaggat ggaaccgctg gagagcaact gcataaggct      180

atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg      240

aacatcacgt acgcggaata cttcgaaatg tccgttcggt tggcagaagc tatgaaacga      300

tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt      360

atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat      420

aatgaacgtg aattgctcaa cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc      480

aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat ccagaaaatt      540

attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca      600

tctcatctac ctcccggttt taatgaatac gattttgtac cagagtcctt tgatcgtgac      660

aaaacaattg cactgataat gaattcctct ggatctactg ggttacctaa gggtgtggcc      720

cttccgcata gaactgcctg cgtcagattc tcgcatgcca gagatcctat ttttggcaat      780

caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg      840

tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa      900

gaagagctgt ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt gctagtacca      960

accctatttt cattcttcgc caaaagcact ctgattgaca aatacgattt atctaattta     1020

cacgaaattg cttctggggg cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa     1080

cgcttccatc ttccagggat acgacaagga tatgggctca ctgagactac atcagctatt     1140

ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt     1200

gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca gagaggcgaa     1260

ttatgtgtca gaggacctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac     1320

gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac     1380

gaacacttct tcatagttga ccgcttgaag tctttaatta aatacaaagg atatcaggtg     1440

gcccccgctg aattggaatc gatattgtta caacacccca acatcttcga cgcgggcgtg     1500

gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac     1560

ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg     1620

aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa     1680

ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagtccaaa     1740

ctcgagtaag gttaacctgc aggagg                                          1766


