               SEQUENCE LISTING

<110> Fraunhofer-Gesellschaft zur Foerderung der angewandten

<120> Novel Immunoproteases

<130> FRA-PA03-EP

<160> 10

<170> BiSSAP 1.2

<210> 1
<211> 696
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..696
<223> /organism="Artificial Sequence"
      /note="wt human granzyme M"
      /mol_type="unassigned DNA"

<400> 1
atcatcgggg gccgggaggt gatcccccac tcgcgcccgt acatggcctc actgcagaga      60

aatggctccc acctgtgcgg gggtgtcctg gtgcacccaa agtgggtgct gacggctgcc     120

cactgcctgg cccagcggat ggcccagctg aggctggtgc tggggctcca caccctggac     180

agccccggtc tcaccttcca catcaaggca gccatccagc accctcgcta caagcccgtc     240

cctgccctgg agaacgacct cgcgctgctt cagctggacg ggaaagtgaa gcccagccgg     300

accatccggc cgttggccct gcccagtaag cgccaggtgg tggcagcagg gactcggtgc     360

agcatggccg gctgggggct gacccaccag ggcgggcgcc tgtcccgggt gctgcgggag     420

ctggacctcc aagtgctgga cacccgcatg tgtaacaaca gccgcttctg gaacggcagc     480

ctctccccca gcatggtctg cctggcggcc gactccaagg accaggctcc ctgcaagggt     540

gactcgggcg ggcccctggt gtgtggcaaa ggccgggtgt tggccggagt cctgtccttc     600

agctccaggg tctgcactga catcttcaag cctcccgtgg ccaccgctgt ggcgccttac     660

gtgtcctgga tcaggaaggt caccggccga tcggcc                               696


<210> 2
<211> 232
<212> PRT
<213> Artificial Sequence

<220> 
<223> wt human granzyme M

<400> 2
Ile Ile Gly Gly Arg Glu Val Ile Pro His Ser Arg Pro Tyr Met Ala 
1               5                   10                  15      
Ser Leu Gln Arg Asn Gly Ser His Leu Cys Gly Gly Val Leu Val His 
            20                  25                  30          
Pro Lys Trp Val Leu Thr Ala Ala His Cys Leu Ala Gln Arg Met Ala 
        35                  40                  45              
Gln Leu Arg Leu Val Leu Gly Leu His Thr Leu Asp Ser Pro Gly Leu 
    50                  55                  60                  
Thr Phe His Ile Lys Ala Ala Ile Gln His Pro Arg Tyr Lys Pro Val 
65                  70                  75                  80  
Pro Ala Leu Glu Asn Asp Leu Ala Leu Leu Gln Leu Asp Gly Lys Val 
                85                  90                  95      
Lys Pro Ser Arg Thr Ile Arg Pro Leu Ala Leu Pro Ser Lys Arg Gln 
            100                 105                 110         
Val Val Ala Ala Gly Thr Arg Cys Ser Met Ala Gly Trp Gly Leu Thr 
        115                 120                 125             
His Gln Gly Gly Arg Leu Ser Arg Val Leu Arg Glu Leu Asp Leu Gln 
    130                 135                 140                 
Val Leu Asp Thr Arg Met Cys Asn Asn Ser Arg Phe Trp Asn Gly Ser 
145                 150                 155                 160 
Leu Ser Pro Ser Met Val Cys Leu Ala Ala Asp Ser Lys Asp Gln Ala 
                165                 170                 175     
Pro Cys Lys Gly Asp Ser Gly Gly Pro Leu Val Cys Gly Lys Gly Arg 
            180                 185                 190         
Val Leu Ala Gly Val Leu Ser Phe Ser Ser Arg Val Cys Thr Asp Ile 
        195                 200                 205             
Phe Lys Pro Pro Val Ala Thr Ala Val Ala Pro Tyr Val Ser Trp Ile 
    210                 215                 220                 
Arg Lys Val Thr Gly Arg Ser Ala 
225                 230         

<210> 3
<211> 1476
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1476
<223> /organism="Artificial Sequence"
      /note="Gm -H22 (scFv)"
      /mol_type="unassigned DNA"

<400> 3
atcatcgggg gccgggaggt gatcccccac tcgcgcccgt acatggcctc actgcagaga      60

aatggctccc acctgtgcgg gggtgtcctg gtgcacccaa agtgggtgct gacggctgcc     120

cactgcctgg cccagcggat ggcccagctg aggctggtgc tggggctcca caccctggac     180

agccccggtc tcaccttcca catcaaggca gccatccagc accctcgcta caagcccgtc     240

cctgccctgg agaacgacct cgcgctgctt cagctggacg ggaaagtgaa gcccagccgg     300

accatccggc cgttggccct gcccagtaag cgccaggtgg tggcagcagg gactcggtgc     360

agcatggccg gctgggggct gacccaccag ggcgggcgcc tgtcccgggt gctgcgggag     420

ctggacctcc aagtgctgga cacccgcatg tgtaacaaca gccgcttctg gaacggcagc     480

ctctccccca gcatggtctg cctggcggcc gactccaagg accaggctcc ctgcaagggt     540

gactcgggcg ggcccctggt gtgtggcaaa ggccgggtgt tggccggagt cctgtccttc     600

agctccaggg tctgcactga catcttcaag cctcccgtgg ccaccgctgt ggcgccttac     660

gtgtcctgga tcaggaaggt caccggccga tcggccgctg agcacgaagg tgacgcggcc     720

cagccggcca tggcccaggt gcagctggtg gagagcggtg gaggtgttgt gcaacctggc     780

cggtccctgc gcctgtcctg ctcctcgtct ggcttcattt tcagtgacaa ttacatgtat     840

tgggtgagac aggcacctgg aaaaggtctt gagtgggttg caaccattag tgatggtggt     900

agttacacct actatccaga cagtgtgaag ggaagattta caatatcgag agacaacagc     960

aagaacacat tgttcctgca aatggacagc ctgagacccg aagacaccgg ggtctatttt    1020

tgtgcaagag gctactatag gtacgagggg gctatggact actggggcca agggaccccg    1080

gtcaccgtga gctcaggagg tggcggctcc ggaggtggag gcagcggagg gggcggatcc    1140

gacatccagc tgacccagag cccaagcagc ctgagcgcca gcgtgggtga cagagtgacc    1200

atcacctgta agtccagtca aagtgtttta tacagttcaa atcagaagaa ctacttggcc    1260

tggtaccagc agaagccagg taaggctcca aagctgctga tctactgggc atccactagg    1320

gaatctggtg tgccaagcag attcagcggt agcggtagcg gtaccgactt caccttcacc    1380

atcagcagcc tccagccaga ggacatcgcc acctactact gccatcaata cctctcctcg    1440

tggacgttcg gccaagggac caagctggag atcaaa                              1476


<210> 4
<211> 492
<212> PRT
<213> Artificial Sequence

<220> 
<223> Gm-H22 (scFv)

<400> 4
Ile Ile Gly Gly Arg Glu Val Ile Pro His Ser Arg Pro Tyr Met Ala 
1               5                   10                  15      
Ser Leu Gln Arg Asn Gly Ser His Leu Cys Gly Gly Val Leu Val His 
            20                  25                  30          
Pro Lys Trp Val Leu Thr Ala Ala His Cys Leu Ala Gln Arg Met Ala 
        35                  40                  45              
Gln Leu Arg Leu Val Leu Gly Leu His Thr Leu Asp Ser Pro Gly Leu 
    50                  55                  60                  
Thr Phe His Ile Lys Ala Ala Ile Gln His Pro Arg Tyr Lys Pro Val 
65                  70                  75                  80  
Pro Ala Leu Glu Asn Asp Leu Ala Leu Leu Gln Leu Asp Gly Lys Val 
                85                  90                  95      
Lys Pro Ser Arg Thr Ile Arg Pro Leu Ala Leu Pro Ser Lys Arg Gln 
            100                 105                 110         
Val Val Ala Ala Gly Thr Arg Cys Ser Met Ala Gly Trp Gly Leu Thr 
        115                 120                 125             
His Gln Gly Gly Arg Leu Ser Arg Val Leu Arg Glu Leu Asp Leu Gln 
    130                 135                 140                 
Val Leu Asp Thr Arg Met Cys Asn Asn Ser Arg Phe Trp Asn Gly Ser 
145                 150                 155                 160 
Leu Ser Pro Ser Met Val Cys Leu Ala Ala Asp Ser Lys Asp Gln Ala 
                165                 170                 175     
Pro Cys Lys Gly Asp Ser Gly Gly Pro Leu Val Cys Gly Lys Gly Arg 
            180                 185                 190         
Val Leu Ala Gly Val Leu Ser Phe Ser Ser Arg Val Cys Thr Asp Ile 
        195                 200                 205             
Phe Lys Pro Pro Val Ala Thr Ala Val Ala Pro Tyr Val Ser Trp Ile 
    210                 215                 220                 
Arg Lys Val Thr Gly Arg Ser Ala Ala Glu His Glu Gly Asp Ala Ala 
225                 230                 235                 240 
Gln Pro Ala Met Ala Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val 
                245                 250                 255     
Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ser Ser Ser Gly Phe 
            260                 265                 270         
Ile Phe Ser Asp Asn Tyr Met Tyr Trp Val Arg Gln Ala Pro Gly Lys 
        275                 280                 285             
Gly Leu Glu Trp Val Ala Thr Ile Ser Asp Gly Gly Ser Tyr Thr Tyr 
    290                 295                 300                 
Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser 
305                 310                 315                 320 
Lys Asn Thr Leu Phe Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr 
                325                 330                 335     
Gly Val Tyr Phe Cys Ala Arg Gly Tyr Tyr Arg Tyr Glu Gly Ala Met 
            340                 345                 350         
Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly Gly 
        355                 360                 365             
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Leu 
    370                 375                 380                 
Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
385                 390                 395                 400 
Ile Thr Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Asn Gln Lys 
                405                 410                 415     
Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
            420                 425                 430         
Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Ser Arg Phe 
        435                 440                 445             
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu 
    450                 455                 460                 
Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys His Gln Tyr Leu Ser Ser 
465                 470                 475                 480 
Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
                485                 490         

<210> 5
<211> 1464
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1464
<223> /organism="Artificial Sequence"
      /note="Gm-Ki4(scFv)"
      /mol_type="unassigned DNA"

<400> 5
atcatcgggg gccgggaggt gatcccccac tcgcgcccgt acatggcctc actgcagaga      60

aatggctccc acctgtgcgg gggtgtcctg gtgcacccaa agtgggtgct gacggctgcc     120

cactgcctgg cccagcggat ggcccagctg aggctggtgc tggggctcca caccctggac     180

agccccggtc tcaccttcca catcaaggca gccatccagc accctcgcta caagcccgtc     240

cctgccctgg agaacgacct cgcgctgctt cagctggacg ggaaagtgaa gcccagccgg     300

accatccggc cgttggccct gcccagtaag cgccaggtgg tggcagcagg gactcggtgc     360

agcatggccg gctgggggct gacccaccag ggcgggcgcc tgtcccgggt gctgcgggag     420

ctggacctcc aagtgctgga cacccgcatg tgtaacaaca gccgcttctg gaacggcagc     480

ctctccccca gcatggtctg cctggcggcc gactccaagg accaggctcc ctgcaagggt     540

gactcgggcg ggcccctggt gtgtggcaaa ggccgggtgt tggccggagt cctgtccttc     600

agctccaggg tctgcactga catcttcaag cctcccgtgg ccaccgctgt ggcgccttac     660

gtgtcctgga tcaggaaggt caccggccga tcggccgctg agcacgaagg tgacgcggcc     720

cagccggcca tggcccaggt caagctgcag gagtcaggga ctgaactggc aaagcctggg     780

gccgcagtga agatgtcctg caaggcttct ggctacacct ttactgacta ctggatgcac     840

tgggttaaac agaggcctgg acagggtctg gaatggattg gatacattaa tcctaacact     900

gcttatactg actacaatca gaaattcaag gacaaggcca cattgactgc agacaaatcc     960

tccagcacag cctacatgca actgcgcagc ctgacctctg aggattctgc agtctattac    1020

tgtgcaaaaa agacaactca gactacgtgg gggtttcctt tttggggcca agggaccacg    1080

gtcaccgtct cctcaggtgg aggcggttca ggcggaggtg gctctggcgg tggcggatcg    1140

gacattgtgc tgacccagtc tccaaaatcc atggccatgt cagtcggaga gagggtcacc    1200

ttgagctgca aggccagtga gaatgtggat tcttttgttt cctggtatca acagaaacca    1260

ggccagtctc ctaaactgct gatatacggg gcctccaacc ggtacactgg ggtccccgat    1320

cgcttcgcag gcagtggatc tggaagagat ttcactctga ccatcagcag tgtgcaggct    1380

gaagaccttg cagattatca ctgtggacag aattacaggt atccgctcac gttcggtgct    1440

ggcaccaagc tggaaatcaa acgg                                           1464


<210> 6
<211> 488
<212> PRT
<213> Artificial Sequence

<220> 
<223> Gm-Ki4(scFv)

<400> 6
Ile Ile Gly Gly Arg Glu Val Ile Pro His Ser Arg Pro Tyr Met Ala 
1               5                   10                  15      
Ser Leu Gln Arg Asn Gly Ser His Leu Cys Gly Gly Val Leu Val His 
            20                  25                  30          
Pro Lys Trp Val Leu Thr Ala Ala His Cys Leu Ala Gln Arg Met Ala 
        35                  40                  45              
Gln Leu Arg Leu Val Leu Gly Leu His Thr Leu Asp Ser Pro Gly Leu 
    50                  55                  60                  
Thr Phe His Ile Lys Ala Ala Ile Gln His Pro Arg Tyr Lys Pro Val 
65                  70                  75                  80  
Pro Ala Leu Glu Asn Asp Leu Ala Leu Leu Gln Leu Asp Gly Lys Val 
                85                  90                  95      
Lys Pro Ser Arg Thr Ile Arg Pro Leu Ala Leu Pro Ser Lys Arg Gln 
            100                 105                 110         
Val Val Ala Ala Gly Thr Arg Cys Ser Met Ala Gly Trp Gly Leu Thr 
        115                 120                 125             
His Gln Gly Gly Arg Leu Ser Arg Val Leu Arg Glu Leu Asp Leu Gln 
    130                 135                 140                 
Val Leu Asp Thr Arg Met Cys Asn Asn Ser Arg Phe Trp Asn Gly Ser 
145                 150                 155                 160 
Leu Ser Pro Ser Met Val Cys Leu Ala Ala Asp Ser Lys Asp Gln Ala 
                165                 170                 175     
Pro Cys Lys Gly Asp Ser Gly Gly Pro Leu Val Cys Gly Lys Gly Arg 
            180                 185                 190         
Val Leu Ala Gly Val Leu Ser Phe Ser Ser Arg Val Cys Thr Asp Ile 
        195                 200                 205             
Phe Lys Pro Pro Val Ala Thr Ala Val Ala Pro Tyr Val Ser Trp Ile 
    210                 215                 220                 
Arg Lys Val Thr Gly Arg Ser Ala Ala Glu His Glu Gly Asp Ala Ala 
225                 230                 235                 240 
Gln Pro Ala Met Ala Gln Val Lys Leu Gln Glu Ser Gly Thr Glu Leu 
                245                 250                 255     
Ala Lys Pro Gly Ala Ala Val Lys Met Ser Cys Lys Ala Ser Gly Tyr 
            260                 265                 270         
Thr Phe Thr Asp Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln 
        275                 280                 285             
Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Asn Thr Ala Tyr Thr Asp 
    290                 295                 300                 
Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser 
305                 310                 315                 320 
Ser Ser Thr Ala Tyr Met Gln Leu Arg Ser Leu Thr Ser Glu Asp Ser 
                325                 330                 335     
Ala Val Tyr Tyr Cys Ala Lys Lys Thr Thr Gln Thr Thr Trp Gly Phe 
            340                 345                 350         
Pro Phe Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
        355                 360                 365             
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu 
    370                 375                 380                 
Thr Gln Ser Pro Lys Ser Met Ala Met Ser Val Gly Glu Arg Val Thr 
385                 390                 395                 400 
Leu Ser Cys Lys Ala Ser Glu Asn Val Asp Ser Phe Val Ser Trp Tyr 
                405                 410                 415     
Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Gly Ala Ser 
            420                 425                 430         
Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Ala Gly Ser Gly Ser Gly 
        435                 440                 445             
Arg Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala 
    450                 455                 460                 
Asp Tyr His Cys Gly Gln Asn Tyr Arg Tyr Pro Leu Thr Phe Gly Ala 
465                 470                 475                 480 
Gly Thr Lys Leu Glu Ile Lys Arg 
                485             

<210> 7
<211> 1464
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1464
<223> /organism="Artificial Sequence"
      /note="Gm-425(scFv)"
      /mol_type="unassigned DNA"

<400> 7
atcatcgggg gccgggaggt gatcccccac tcgcgcccgt acatggcctc actgcagaga      60

aatggctccc acctgtgcgg gggtgtcctg gtgcacccaa agtgggtgct gacggctgcc     120

cactgcctgg cccagcggat ggcccagctg aggctggtgc tggggctcca caccctggac     180

agccccggtc tcaccttcca catcaaggca gccatccagc accctcgcta caagcccgtc     240

cctgccctgg agaacgacct cgcgctgctt cagctggacg ggaaagtgaa gcccagccgg     300

accatccggc cgttggccct gcccagtaag cgccaggtgg tggcagcagg gactcggtgc     360

agcatggccg gctgggggct gacccaccag ggcgggcgcc tgtcccgggt gctgcgggag     420

ctggacctcc aagtgctgga cacccgcatg tgtaacaaca gccgcttctg gaacggcagc     480

ctctccccca gcatggtctg cctggcggcc gactccaagg accaggctcc ctgcaagggt     540

gactcgggcg ggcccctggt gtgtggcaaa ggccgggtgt tggccggagt cctgtccttc     600

agctccaggg tctgcactga catcttcaag cctcccgtgg ccaccgctgt ggcgccttac     660

gtgtcctgga tcaggaaggt caccggccga tcggccgctg agcacgaagg tgacgcggcc     720

cagccggcca tggcgcaggt gcaactgcag cagtctgggg ctgaactggt gaagcctggg     780

gcttcagtga agttgtcctg caaggcttcc ggctacacct tcaccagcca ctggatgcac     840

tgggtgaagc agagggctgg acaaggcctt gagtggatcg gagagtttaa tcccagcaac     900

ggccgtacta actacaatga gaaattcaag agcaaggcca cactgactgt agacaaatcc     960

tccagcacag cctacatgca actcagcagc ctgacatctg aggactctgc ggtctattac    1020

tgtgccagtc gggactatga ttacgacgga cggtactttg actactgggg ccaagggacc    1080

acggtcaccg tctcctcagg tggcggtggc tcgggcggtg gtgggtcggg tggcggcgga    1140

tctgacatcg agctcaccca gtctccagca atcatgtctg catctccagg ggagaaggtc    1200

actatgacct gcagtgccag ctcaagtgta acttacatgt attggtacca gcagaagcca    1260

ggatcctccc ccagactcct gatttatgac acatccaacc tggcttctgg agtccctgtt    1320

cgtttcagtg gcagtgggtc tgggacctct tactctctca caatcagccg aatggaggct    1380

gaagatgctg ccacttatta ctgccagcag tggagtagtc acatattcac gttcggctcg    1440

gggacagaac tcgagatcaa acgg                                           1464


<210> 8
<211> 488
<212> PRT
<213> Artificial Sequence

<220> 
<223> Gm-425(scFv)

<400> 8
Ile Ile Gly Gly Arg Glu Val Ile Pro His Ser Arg Pro Tyr Met Ala 
1               5                   10                  15      
Ser Leu Gln Arg Asn Gly Ser His Leu Cys Gly Gly Val Leu Val His 
            20                  25                  30          
Pro Lys Trp Val Leu Thr Ala Ala His Cys Leu Ala Gln Arg Met Ala 
        35                  40                  45              
Gln Leu Arg Leu Val Leu Gly Leu His Thr Leu Asp Ser Pro Gly Leu 
    50                  55                  60                  
Thr Phe His Ile Lys Ala Ala Ile Gln His Pro Arg Tyr Lys Pro Val 
65                  70                  75                  80  
Pro Ala Leu Glu Asn Asp Leu Ala Leu Leu Gln Leu Asp Gly Lys Val 
                85                  90                  95      
Lys Pro Ser Arg Thr Ile Arg Pro Leu Ala Leu Pro Ser Lys Arg Gln 
            100                 105                 110         
Val Val Ala Ala Gly Thr Arg Cys Ser Met Ala Gly Trp Gly Leu Thr 
        115                 120                 125             
His Gln Gly Gly Arg Leu Ser Arg Val Leu Arg Glu Leu Asp Leu Gln 
    130                 135                 140                 
Val Leu Asp Thr Arg Met Cys Asn Asn Ser Arg Phe Trp Asn Gly Ser 
145                 150                 155                 160 
Leu Ser Pro Ser Met Val Cys Leu Ala Ala Asp Ser Lys Asp Gln Ala 
                165                 170                 175     
Pro Cys Lys Gly Asp Ser Gly Gly Pro Leu Val Cys Gly Lys Gly Arg 
            180                 185                 190         
Val Leu Ala Gly Val Leu Ser Phe Ser Ser Arg Val Cys Thr Asp Ile 
        195                 200                 205             
Phe Lys Pro Pro Val Ala Thr Ala Val Ala Pro Tyr Val Ser Trp Ile 
    210                 215                 220                 
Arg Lys Val Thr Gly Arg Ser Ala Ala Glu His Glu Gly Asp Ala Ala 
225                 230                 235                 240 
Gln Pro Ala Met Ala Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu 
                245                 250                 255     
Val Lys Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr 
            260                 265                 270         
Thr Phe Thr Ser His Trp Met His Trp Val Lys Gln Arg Ala Gly Gln 
        275                 280                 285             
Gly Leu Glu Trp Ile Gly Glu Phe Asn Pro Ser Asn Gly Arg Thr Asn 
    290                 295                 300                 
Tyr Asn Glu Lys Phe Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser 
305                 310                 315                 320 
Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
                325                 330                 335     
Ala Val Tyr Tyr Cys Ala Ser Arg Asp Tyr Asp Tyr Asp Gly Arg Tyr 
            340                 345                 350         
Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly 
        355                 360                 365             
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Glu 
    370                 375                 380                 
Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val 
385                 390                 395                 400 
Thr Met Thr Cys Ser Ala Ser Ser Ser Val Thr Tyr Met Tyr Trp Tyr 
                405                 410                 415     
Gln Gln Lys Pro Gly Ser Ser Pro Arg Leu Leu Ile Tyr Asp Thr Ser 
            420                 425                 430         
Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly 
        435                 440                 445             
Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu Asp Ala Ala 
    450                 455                 460                 
Thr Tyr Tyr Cys Gln Gln Trp Ser Ser His Ile Phe Thr Phe Gly Ser 
465                 470                 475                 480 
Gly Thr Glu Leu Glu Ile Lys Arg 
                485             

<210> 9
<211> 1446
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1446
<223> /organism="Artificial Sequence"
      /note="Gm-RFT5(scFv)"
      /mol_type="unassigned DNA"

<400> 9
atcatcgggg gccgggaggt gatcccccac tcgcgcccgt acatggcctc actgcagaga      60

aatggctccc acctgtgcgg gggtgtcctg gtgcacccaa agtgggtgct gacggctgcc     120

cactgcctgg cccagcggat ggcccagctg aggctggtgc tggggctcca caccctggac     180

agccccggtc tcaccttcca catcaaggca gccatccagc accctcgcta caagcccgtc     240

cctgccctgg agaacgacct cgcgctgctt cagctggacg ggaaagtgaa gcccagccgg     300

accatccggc cgttggccct gcccagtaag cgccaggtgg tggcagcagg gactcggtgc     360

agcatggccg gctgggggct gacccaccag ggcgggcgcc tgtcccgggt gctgcgggag     420

ctggacctcc aagtgctgga cacccgcatg tgtaacaaca gccgcttctg gaacggcagc     480

ctctccccca gcatggtctg cctggcggcc gactccaagg accaggctcc ctgcaagggt     540

gactcgggcg ggcccctggt gtgtggcaaa ggccgggtgt tggccggagt cctgtccttc     600

agctccaggg tctgcactga catcttcaag cctcccgtgg ccaccgctgt ggcgccttac     660

gtgtcctgga tcaggaaggt caccggccga tcggccgctg agcacgaagg tgacgcggcc     720

cagccggccc aggtgaagct ggaggagtca gggactgtgc tggcaaggcc tggggcttcc     780

gtgaagatgt cctgcaaggc ttctggctac aggtttacca actactggat gcactgggta     840

aaacagaggc ctggacaggg tctagaatgg attggtgtta tttatcctgg aaatagtgat     900

actagctaca accagaagtt caagggcaag gccaaactga ctgcagtcac atccgccagc     960

actgcctaca tggagctcag cagcctgaca aatgaggact ctgcggtcta ttactgtaca    1020

agagagggag aaggctctga ctactggggc caagggacca cggtcaccgt ctcctcaggt    1080

ggaggcggtt caggcggagg tggctctggc ggtggcggat cgcaaattgt tctcacccag    1140

tctccagcaa ccatggctgc atctcccggg gagaagatca ctatcacctg cagtgccagc    1200

tcaagtataa gttccaatta cttgcattgg tatcagcaga agccaggatt ctcccctaaa    1260

ctcttgattt ataggacttc caatctggct tctggagtcc cagctcgctt cagtggcagt    1320

gggtctggga cctcttactc tctcacaatt ggcaccatgg aggctgaaga tgttgccact    1380

tactactgcc agcagggtag tagtataccg tacacgttcg gaggggggac caagctggag    1440

ctcaaa                                                               1446


<210> 10
<211> 487
<212> PRT
<213> Artificial Sequence

<220> 
<223> Gm-RFT5(scFv)

<400> 10
Ile Ile Gly Gly Arg Glu Val Ile Pro His Ser Arg Pro Tyr Met Ala 
1               5                   10                  15      
Ser Leu Gln Arg Asn Gly Ser His Leu Cys Gly Gly Val Leu Val His 
            20                  25                  30          
Pro Lys Trp Val Leu Thr Ala Ala His Cys Leu Ala Gln Arg Met Ala 
        35                  40                  45              
Gln Leu Arg Leu Val Leu Gly Leu His Thr Leu Asp Ser Pro Gly Leu 
    50                  55                  60                  
Thr Phe His Ile Lys Ala Ala Ile Gln His Pro Arg Tyr Lys Pro Val 
65                  70                  75                  80  
Pro Ala Leu Glu Asn Asp Leu Ala Leu Leu Gln Leu Asp Gly Lys Val 
                85                  90                  95      
Lys Pro Ser Arg Thr Ile Arg Pro Leu Ala Leu Pro Ser Lys Arg Gln 
            100                 105                 110         
Val Val Ala Ala Gly Thr Arg Cys Ser Met Ala Gly Trp Gly Leu Thr 
        115                 120                 125             
His Gln Gly Gly Arg Leu Ser Arg Val Leu Arg Glu Leu Asp Leu Gln 
    130                 135                 140                 
Val Leu Asp Thr Arg Met Cys Asn Asn Ser Arg Phe Trp Asn Gly Ser 
145                 150                 155                 160 
Leu Ser Pro Ser Met Val Cys Leu Ala Ala Asp Ser Lys Asp Gln Ala 
                165                 170                 175     
Pro Cys Lys Gly Asp Ser Gly Gly Pro Leu Val Cys Gly Lys Gly Arg 
            180                 185                 190         
Val Leu Ala Gly Val Leu Ser Phe Ser Ser Arg Val Cys Thr Asp Ile 
        195                 200                 205             
Phe Lys Pro Pro Val Ala Thr Ala Val Ala Pro Tyr Val Ser Trp Ile 
    210                 215                 220                 
Arg Lys Val Thr Gly Arg Ser Ala Ala Glu His Glu Gly Asp Ala Ala 
225                 230                 235                 240 
Gln Pro Ala Gln Val Lys Leu Glu Glu Ser Gly Thr Val Leu Ala Arg 
                245                 250                 255     
Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Arg Phe 
            260                 265                 270         
Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
        275                 280                 285             
Glu Trp Ile Gly Val Ile Tyr Pro Gly Asn Ser Asp Thr Ser Tyr Asn 
    290                 295                 300                 
Gln Lys Phe Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Ala Ser 
305                 310                 315                 320 
Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val 
                325                 330                 335     
Tyr Tyr Cys Thr Arg Glu Gly Glu Gly Ser Asp Tyr Trp Gly Gln Gly 
            340                 345                 350         
Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        355                 360                 365             
Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln Ser Pro Ala Thr 
    370                 375                 380                 
Met Ala Ala Ser Pro Gly Glu Lys Ile Thr Ile Thr Cys Ser Ala Ser 
385                 390                 395                 400 
Ser Ser Ile Ser Ser Asn Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly 
                405                 410                 415     
Phe Ser Pro Lys Leu Leu Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly 
            420                 425                 430         
Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 
        435                 440                 445             
Thr Ile Gly Thr Met Glu Ala Glu Asp Val Ala Thr Tyr Tyr Cys Gln 
    450                 455                 460                 
Gln Gly Ser Ser Ile Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu 
465                 470                 475                 480 
Leu Lys Ala Ala Ala Gly Pro 
                485         

