                         SEQUENCE LISTING

<110>  Georgia State University Research Foundation, Inc.
       Kang , Sang-Moo
       Kim , Min-Chul
 
<120>  UNIVERSAL INFLUENZA VACCINE BASED ON HETEROLOGOUS MULTIPLE M2E 
       PROTEINS

<130>  10013-025WO1

<150>  US 61/722,602
<151>  2012-11-05

<150>  US 61/738,139
<151>  2012-12-17

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1

Pro Ile Arg Asn Glu Trp Gly Ser Arg Ser Asn 
1               5                   10      


<210>  2
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Pro Thr Arg Ser Glu Trp Glu Ser Arg Ser Ser 
1               5                   10      


<210>  3
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Asn, His, or Lys

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Glu or Gly

<400>  3

Pro Thr Arg Xaa Xaa Trp Glu Ser Arg Ser Ser 
1               5                   10      


<210>  4
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Leu Thr Arg Asn Gly Trp Gly Cys Arg Cys Ser 
1               5                   10      


<210>  5
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5

Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Ser 
1               5                   10                  15      


Arg Ser Asn Asp Ser Ser Asp 
            20              


<210>  6
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Ser Glu Trp Glu Ser 
1               5                   10                  15      


Arg Ser Ser Asp Ser Ser Asp 
            20              


<210>  7
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7

Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Glu Trp Glu Ser 
1               5                   10                  15      


Arg Ser Ser Asp Ser Ser Asp 
            20              


<210>  8
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Gly Trp Glu Cys 
1               5                   10                  15      


Arg Cys Ser Asp Ser Ser Asp 
            20              


<210>  9
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  9

Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg His Glu Trp Glu Cys 
1               5                   10                  15      


Arg Cys Ser Asp Ser Ser Asp 
            20              


<210>  10
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Lys Glu Trp Glu Cys 
1               5                   10                  15      


Arg Cys Ser Asp Ser Ser Asp 
            20              


<210>  11
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11

Ser Leu Leu Thr Glu Val Glu Thr Leu Thr Arg Asn Gly Trp Gly Cys 
1               5                   10                  15      


Arg Cys Ser Asp Ser Ser Asp 
            20              


<210>  12
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Asp Pro Ile Asn Met Thr 
            20                  25          


<210>  13
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Gly Gly Leu Lys Gln Ile Glu Asp Lys Leu Glu Glu Ile Leu Ser Lys 
1               5                   10                  15      


Leu Tyr His Ile Glu Asn Glu Leu Ala Arg Ile Lys Lys Leu Leu Gly 
            20                  25                  30          


Glu 
    


<210>  14
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val 
1               5                   10                  15      


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
            20                  25                  30          


Cys Arg Ile Cys Ile 
        35          


<210>  15
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Ser Ser Leu Pro Tyr 
1               5                   10                  15      


Gln Asn Ile His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg 
            20                  25                  30          


Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn Asn Pro Ser Ile 
        35                  40                  45              


Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly 
    50                  55                  60                  


Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu 
65                  70                  75                  80  


Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile 
                85                  90                  95      


Asn Gly Ile Thr Asn Lys Val Asn Thr Val Ile Glu Lys Met Asn Ile 
            100                 105                 110         


Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Lys Arg Met 
        115                 120                 125             


Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr 
    130                 135                 140                 


Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp 
145                 150                 155                 160 


Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln 
                165                 170                 175     


Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr 
            180                 185                 190         


His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn Gly Thr Tyr 
        195                 200                 205             


Asp Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Val 
    210                 215                 220                 


Asp Gly Val Lys Leu Glu Ser Met Gly Ile Tyr Gln Ile Leu Ala Ile 
225                 230                 235                 240 


Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val Ser Leu Gly Ala 
                245                 250                 255     


Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys 
            260                 265                 270         


Ile 
    


<210>  16
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Asp Pro Ile Asn Met Thr Thr Ser Val Asp Gly 
            20                  25                  30          


Thr Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
        35                  40                  45              


Ser Arg Ser Asn Asp Ser Ser Asp Ala Ala Ala Gly Gly Ala Ala Ser 
    50                  55                  60                  


Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Ser Arg 
65                  70                  75                  80  


Ser Asn Asp Ser Ser Asp Ala Ala Ala Pro Gly Ala Ala Ser Leu Leu 
                85                  90                  95      


Thr Glu Val Glu Thr Pro Thr Arg Ser Glu Trp Glu Ser Arg Ser Ser 
            100                 105                 110         


Asp Ser Ser Asp Ala Ala Ala Gly Gly Ala Ala Ser Leu Leu Thr Glu 
        115                 120                 125             


Val Glu Thr Pro Thr Arg Asn Glu Trp Glu Ser Arg Ser Ser Asp Ser 
    130                 135                 140                 


Ser Asp Ala Ala Ala Pro Gly Ala Ala Ser Leu Leu Thr Glu Val Glu 
145                 150                 155                 160 


Thr Leu Thr Arg Asn Gly Trp Gly Cys Arg Cys Ser Asp Ser Ser Asp 
                165                 170                 175     


Gly Gly Leu Lys Gln Ile Glu Asp Lys Leu Glu Glu Ile Leu Ser Lys 
            180                 185                 190         


Leu Tyr His Ile Glu Asn Glu Leu Ala Arg Ile Lys Lys Leu Leu Gly 
        195                 200                 205             


Glu Leu Glu Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val 
    210                 215                 220                 


Leu Leu Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly 
225                 230                 235                 240 


Ser Leu Gln Cys Arg Ile Cys Ile 
                245             


<210>  17
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala Ala His 
1               5                   10                  15      


Ser Ala Phe Ala 
            20  


<210>  18
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18

Met Pro Leu Tyr Lys Leu Leu Asn Val Leu Trp Leu Val Ala Val Ser 
1               5                   10                  15      


Asn Ala Ile Pro 
            20  


<210>  19
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  19

Gln Met Asp Arg Val Val Lys Glu Met Arg Arg Gln Leu Glu Met Ile 
1               5                   10                  15      


Asp Lys Leu Thr Thr Arg Glu Ile Glu Gln Val Glu Leu Leu Lys Arg 
            20                  25                  30          


Ile Tyr Asp Lys Leu 
        35          


<210>  20
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  20

Leu Asn Pro Leu Asp Trp Thr Gln Tyr Phe Ile Phe Ile Gly Val Gly 
1               5                   10                  15      


Ala Leu Leu Leu Val Ile Val Leu Met Ile Phe Pro Ile Val Phe Gln 
            20                  25                  30          


Cys Leu Ala Lys Ser Leu Asp Gln Val Gln Ser Asp Leu Asn Val Leu 
        35                  40                  45              


Leu Leu Lys Lys Lys Lys Gly Gly Asn Ala Ala Pro Ala Ala Glu Met 
    50                  55                  60                  


Val Glu Leu Pro Arg Val Ser Tyr Thr 
65                  70              


<210>  21
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  21

Phe Met Phe Gly His Val Val Asn Phe Val Ile Ile Leu Ile Val Ile 
1               5                   10                  15      


Leu Phe Leu Tyr Cys Met Ile Arg Asn Arg Asn Arg Gln Tyr 
            20                  25                  30  


<210>  22
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  22

Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ala Val Gln Ser Ala 
1               5                   10                  15      


Asn Ser 
        


<210>  23
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  23

Ala Ala Pro Gly Ala Ala 
1               5       


<210>  24
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  24

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Asp Pro Ile Asn Met Thr Thr Ser Ile Asn Asn 
            20                  25                  30          


Asn Leu Gln Arg Val Arg Glu Leu Ala Val Gln Ser Ala Asn Ser Ala 
        35                  40                  45              


Ala Ala Pro Gly Ala Ala Val Asp Gly Thr Ser Leu Leu Thr Glu Val 
    50                  55                  60                  


Glu Thr Pro Thr Arg Asn Glu Trp Glu Ser Arg Ser Ser Asp Ser Ser 
65                  70                  75                  80  


Asp Ala Ala Ala Gly Gly Ala Ala Ser Leu Leu Thr Glu Val Glu Thr 
                85                  90                  95      


Pro Thr Arg Asn Glu Trp Glu Ser Arg Ser Ser Asp Ser Ser Asp Ala 
            100                 105                 110         


Ala Ala Pro Gly Ala Ala Ser Leu Leu Thr Glu Val Glu Thr Pro Thr 
        115                 120                 125             


Arg His Glu Trp Glu Ser Arg Ser Ser Asp Ser Ser Asp Ala Ala Ala 
    130                 135                 140                 


Gly Gly Ala Ala Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg His 
145                 150                 155                 160 


Glu Trp Glu Ser Arg Ser Ser Asp Ser Ser Asp Ala Ala Ala Pro Gly 
                165                 170                 175     


Ala Ala Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Gly Trp 
            180                 185                 190         


Lys Cys Lys Cys Ser Asp Ser Ser Asp Gly Gly Leu Lys Gln Ile Glu 
        195                 200                 205             


Asp Lys Leu Glu Glu Ile Leu Ser Lys Leu Tyr His Ile Glu Asn Glu 
    210                 215                 220                 


Leu Ala Arg Ile Lys Lys Leu Leu Gly Glu Leu Glu Ile Leu Ala Ile 
225                 230                 235                 240 


Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val Ser Leu Gly Ala 
                245                 250                 255     


Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys 
            260                 265                 270         


Ile 
    


<210>  25
<211>  819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  25
atgaagtttc tggtgaacgt tgccctggtt tttatggttg tttacatttc ctacatttac       60

gctgacccga ttaacatgac gactagtatt aacaataacc tgcagcgtgt ccgcgaactg      120

gccgtgcaaa gtgcaaattc cgcagcagca ccgggtgctg cagtcgacgg taccagcctg      180

ctgacggaag tcgaaacccc gacgcgtaac gaatgggaat ctcgcagctc tgatagttcc      240

gacgcagcag ctggcggtgc agccagtctg ctgaccgaag tggaaacccc gacgcgtaat      300

gaatgggaat cccgctcatc ggatagctct gacgcagctg caccgggtgc agcatcactg      360

ctgacggaag ttgaaacccc gacgcgtcat gaatgggaat cgcgcagttc cgattcatcg      420

gacgctgcag ccggcggtgc agccagcctg ctgaccgaag ttgaaacgcc gacccgtcat      480

gaatgggaaa gccgttctag cgacagttcc gatgccgcag caccgggtgc tgcgagcctg      540

ctgaccgaag tcgaaacgcc gacgcgtaat ggttggaaat gcaagtgtag cgattcatcg      600

gacggcggtc tgaaacagat tgaagataag ctggaagaaa tcctgagtaa actgtaccat      660

atcgaaaacg aactggcgcg catcaaaaag ctgctgggcg aactcgagat tctggccatc      720

tactcaaccg tggcgagctc tctggtgctg ctggtttcgc tgggtgcaat ctccttctgg      780

atgtgttcta acggttccct gcaatgtcgt atctgtatc                             819


<210>  26
<211>  771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  26
gccgccacca tgagtcttct aaccgaggtc gaaacgtacg ttctctctat catcccgtca       60

ggccccctca aagccgagat cgcacagaga cttgaagatg tctttgcagg gaagaacacc      120

gatcttgagg ttctcatgga atggctaaag acaagaccaa tcctgtcacc tctgactaag      180

gggattttag gatttgtgtt cacgctcacc gtgcccagtg agcgaggact acagcgtaga      240

cgctttgtcc aaaatgccct taatgggaac ggcgatccaa ataacatgga caaagcagtt      300

aaactgtata ggaagctcaa gagggagata acatttcatg gggccaaaga aatctcactc      360

agttattctg ctggtgcact tgccagttgt atgggcctca tatacaacag gatgggggct      420

gtgaccactg aagtggcatt tggcctggta tgtgcaacct gtgaacagat tgctgactcc      480

cagcatcggt ctcataggca aatggtgaca acaaccaacc cactaatcag acatgagaac      540

agaatggttt tagccagcac tacagctaag gctatggagc aaatggctgg atcgagtgag      600

caagcagcag aggctatgga ggttgctagt caggctaggc aaatggtgca agcgatgaga      660

accattggga ctcatcctag ctccagtgct ggtctgaaaa atgatcttct tgaaaatttg      720

caggcctatc agaaacgaat gggggtgcag atgcaacggt tcaagtgatg a               771


<210>  27
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27

Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Ile Pro 
1               5                   10                  15      


Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe 
            20                  25                  30          


Ala Gly Lys Asn Thr Asp Leu Glu Val Leu Met Glu Trp Leu Lys Thr 
        35                  40                  45              


Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe 
    50                  55                  60                  


Thr Leu Thr Val Pro Ser Glu Arg Gly Leu Gln Arg Arg Arg Phe Val 
65                  70                  75                  80  


Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Met Asp Lys Ala 
                85                  90                  95      


Val Lys Leu Tyr Arg Lys Leu Lys Arg Glu Ile Thr Phe His Gly Ala 
            100                 105                 110         


Lys Glu Ile Ser Leu Ser Tyr Ser Ala Gly Ala Leu Ala Ser Cys Met 
        115                 120                 125             


Gly Leu Ile Tyr Asn Arg Met Gly Ala Val Thr Thr Glu Val Ala Phe 
    130                 135                 140                 


Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg 
145                 150                 155                 160 


Ser His Arg Gln Met Val Thr Thr Thr Asn Pro Leu Ile Arg His Glu 
                165                 170                 175     


Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met 
            180                 185                 190         


Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Glu Val Ala Ser Gln 
        195                 200                 205             


Ala Arg Gln Met Val Gln Ala Met Arg Thr Ile Gly Thr His Pro Ser 
    210                 215                 220                 


Ser Ser Ala Gly Leu Lys Asn Asp Leu Leu Glu Asn Leu Gln Ala Tyr 
225                 230                 235                 240 


Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys 
                245                 250         


<210>  28
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  28

Met Lys Ala Asn Leu Leu Val Leu Leu Cys Ala Leu Ala Ala Ala Asp 
1               5                   10                  15      


Ala 
    


