
                                SEQUENCE LISTING

<110> Regents of the University of Minnesota

<120> FACTOR IX VARIANTS
  

<130> 09531-0331WO1

<150> 61/719,576          
<151> 2012-10-29  

<160> 42

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 45
<212> PRT
<213> Homo sapiens

<220> 
<221> VARIANT        
<222> 7, 8, 15, 17, 20, 21, 26, 27, 30, 33, 36, 40
<223> Xaa = gamma carboxyglutamic acid

<400> 1
Tyr Asn Ser Gly Lys Leu Xaa Xaa Phe Val Gln Gly Asn Leu Xaa Arg
 1               5                  10                  15      
Xaa Cys Met Xaa Xaa Lys Cys Ser Phe Xaa Xaa Ala Arg Xaa Val Phe
            20                  25                  30          
Xaa Asn Thr Xaa Arg Thr Thr Xaa Phe Trp Lys Gln Tyr
        35                  40                  45  


<210> 2
<211> 46
<212> PRT
<213> Bos taurus

<220> 
<221> VARIANT        
<222> 7, 8, 15, 17, 20, 21, 26, 27, 30, 33, 36, 41
<223> Xaa = gamma carboxyglutamic acid

<400> 2
Tyr Asn Ser Gly Lys Leu Xaa Xaa Phe Val Gln Gly Asn Leu Xaa Arg
 1               5                  10                  15      
Xaa Cys Met Xaa Xaa Lys Cys Ser Phe Xaa Xaa Ala Arg Xaa Val Phe
            20                  25                  30          
Xaa Asn Thr Xaa Lys Arg Thr Thr Xaa Phe Trp Lys Gln Tyr
        35                  40                  45      


<210> 3
<211> 461
<212> PRT
<213> Homo sapiens

<400> 3
Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr
 1               5                  10                  15      
Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu
            20                  25                  30          
Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn
        35                  40                  45              
Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys
    50                  55                  60                  
Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn
65                  70                  75                  80  
Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln
                85                  90                  95      
Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile
            100                 105                 110         
Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys
        115                 120                 125             
Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe
    130                 135                 140                 
Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly
145                 150                 155                 160 
Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe
                165                 170                 175     
Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala
            180                 185                 190         
Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu
        195                 200                 205             
Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe
    210                 215                 220                 
Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp
225                 230                 235                 240 
Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile
                245                 250                 255     
Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly
            260                 265                 270         
Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu
        275                 280                 285             
His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn
    290                 295                 300                 
Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu
305                 310                 315                 320 
Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile
                325                 330                 335     
Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr
            340                 345                 350         
Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val
        355                 360                 365             
Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Arg
    370                 375                 380                 
Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His
385                 390                 395                 400 
Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val
                405                 410                 415     
Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly
            420                 425                 430         
Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser
        435                 440                 445             
Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr
    450                 455                 460     


<210> 4
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 4
ttcatatttt ttggaagagt ttgttcaagg gaa                              33

<210> 5
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 5
caaaaaatat gaattatacc tctttggc                                    28

<210> 6
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 6
ttgaattcac tgaaagaaca actga                                       25

<210> 7
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 7
tttcagtgaa ttcaaaaact tctcgtgctt                                  30

<210> 8
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 8
gctaattcat attttttgga agagtttgtt ca                               32

<210> 9
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 9
aaatatgaat tagccctctt tggccgattc agaat                            35

<210> 10
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 10
gctaattcat atttattgga agagtttgtt ca                               32

<210> 11
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 11
taaatatgaa ttagccctct ttggccgatt cagaat                           36

<210> 12
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 12
gctaattcag gatttttgga agagtttgtt ca                               32

<210> 13
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 13
aaatcctgaa ttagccctct ttggccgatt cagaatt                          37

<210> 14
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 14
gaagaagctt gtagttttga agaagcacga gaa                              33

<210> 15
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 15
ctacaagctt cttccataca ttctctctca agg                              33

<210> 16
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 16
gtttaagcaa gggaaccttg agagagaatg t                                31

<210> 17
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 17
ttcccttgct taaactcttc caaaaaatat gaat                             34

<210> 18
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 18
gctaattcag gtaaattgga agagtttgtt caaggg                           36

<210> 19
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 19
tttacctgaa ttagccctct ttggccgatt cagaattt                         38

<210> 20
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 20
ttcaggtctt ttggaagagt ttgttcaagg gaa                              33

<210> 21
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 21
caaaagacct gaattatacc tctttggccg att                              33

<210> 22
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 22
gctaattcat ataaattgga agagtttgtt caagggaa                         38

<210> 23
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 23
tttatatgaa ttagccctct ttggccgatt cagaattt                         38

<210> 24
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 24
taattcatat aaattggaag agtttgttca agggaa                           36

<210> 25
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 25
caatttatat gaattatacc tctttggccg attc                             34

<210> 26
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 26
taattcatat cttttggaag agtttgttca agggaa                           36

<210> 27
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 27
caaaagatat gaattatacc tctttggccg attc                             34

<210> 28
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 28
gctaattcag gacttttgga agagtttgtt ca                               32

<210> 29
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 29
aagtcctgaa ttagccctct ttggccgatt cagaatt                          37

<210> 30
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 30
tgttagctgg ggtgaagggt gtgcaatgaa aggcaaatat                       40

<210> 31
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 31
cccttcaccc cagctaacaa ttccagttaa gaaact                           36

<210> 32
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 32
attaatacct acaaccatga cattgccctt ctg                              33

<210> 33
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 33
ggttgtaggt attaatagct gcattgtagt tgtg                             34

<210> 34
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 34
aaatggcact gctggttcac a                                           21

<210> 35
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 35
aatgggcgtg gatagcggtt tgact                                       25

<210> 36
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 36
agggatatcg acttgcagaa aacc                                        24

<210> 37
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 37
tgctgacaag gaatacacga aca                                         23

<210> 38
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 38
ctttccgcct cagaagccat agag                                        24

<210> 39
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 39
tggcaactag aaggcacagt cg                                          22

<210> 40
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 40
agactcttcc ccagccactt acat                                        24

<210> 41
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 41
aaatggcact gctggttcac agg                                         23

<210> 42
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 42
taatacgact cactataggg                                             20
