                         SEQUENCE LISTING

<110>  Cancer Research Technology Limited
 
<120>  Anti-S100A4 Antibody Molecules and Their Uses

<130>  SJK/BP6938328

<150>  GB 1219487.4
<151>  2012-10-30

<160>  30    

<170>  PatentIn version 3.3

<210>  1
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  1

Gly Asp Ser Phe Thr Asn Asp Tyr Tyr Trp Asn 
1               5                   10      


<210>  2
<211>  16
<212>  PRT
<213>  Mus musculus

<400>  2

His Ile Gly Tyr Gly Gly Asn Ile Asn Tyr Asn Pro Ser Leu Lys Asn 
1               5                   10                  15      


<210>  3
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  3

Glu Ser Phe Tyr Asp Gly Tyr Pro Phe Asp Tyr 
1               5                   10      


<210>  4
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  4

Arg Ala Ser Gln Asp Ile Arg Asn Tyr Leu Asn 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  5

Tyr Thr Ser Arg Leu His Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  6

Gln Gln Gly Asn Ser Leu Pro Arg Thr 
1               5                   


<210>  7
<211>  138
<212>  PRT
<213>  Mus musculus

<400>  7

Met Lys Val Leu Ser Leu Leu Tyr Leu Leu Thr Ala Ile Pro Gly Ile 
1               5                   10                  15      


Leu Ser Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro 
            20                  25                  30          


Ser Gln Ser Leu Ser Leu Thr Cys Ser Val Thr Gly Asp Ser Phe Thr 
        35                  40                  45              


Asn Asp Tyr Tyr Trp Asn Trp Ile Arg Gln Phe Pro Gly Ser Lys Leu 
    50                  55                  60                  


Glu Trp Met Gly His Ile Gly Tyr Gly Gly Asn Ile Asn Tyr Asn Pro 
65                  70                  75                  80  


Ser Leu Lys Asn Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln 
                85                  90                  95      


Phe Phe Leu Arg Leu Thr Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr 
            100                 105                 110         


Tyr Cys Thr Arg Glu Ser Phe Tyr Asp Gly Tyr Pro Phe Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
    130                 135             


<210>  8
<211>  414
<212>  DNA
<213>  Mus musculus

<400>  8
atgaaagtgt tgagtctgtt gtacctgttg acagccattc ctggtatcct gtctgatgta     60

cagcttcagg agtcaggacc tggcctcgtg aaaccttctc agtctctgtc tctcacctgc    120

tctgtcactg gcgactcctt caccaatgat tattactgga actggatccg gcagtttcca    180

ggaagcaaac tggaatggat gggccacata ggctacggcg gtaacattaa ctacaaccca    240

tctctcaaaa atcgaatctc catcactcgt gacacatcta agaaccaatt tttcctgagg    300

ttgacttctg tgactactga ggacacagct acatattact gtacaagaga gagtttctat    360

gatggttacc cctttgatta ctggggccaa gggactctgg tcactgtctc tgca          414


<210>  9
<211>  127
<212>  PRT
<213>  Mus musculus

<400>  9

Met Met Ser Ser Ala Gln Phe Leu Gly Leu Leu Leu Leu Cys Phe Gln 
1               5                   10                  15      


Gly Thr Arg Cys Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp 
        35                  40                  45              


Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Arg Pro Gly Gly Thr Leu 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser 
                85                  90                  95      


Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn 
            100                 105                 110         


Ser Leu Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125         


<210>  10
<211>  381
<212>  DNA
<213>  Mus musculus

<400>  10
atgatgtcct ctgctcagtt ccttggtctc ctgttgctct gttttcaagg taccagatgt     60

gatatccaga tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc    120

atcagttgca gggcaagtca ggacattagg aattatttaa actggtatca gcagagacca    180

ggtggaactc ttaaactcct gatctactac acatcaagat tacactcagg agtcccatca    240

aggttcagtg gcagtgggtc tggaacagat tattctctca ccattagtaa cctggaacaa    300

gaagatattg ccacttactt ttgccaacag ggtaattcgc ttcctcggac gttcggtgga    360

ggcaccaagc tggaaatcaa a                                              381


<210>  11
<211>  101
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Ala Cys Pro Leu Glu Lys Ala Leu Asp Val Met Val Ser Thr Phe 
1               5                   10                  15      


His Lys Tyr Ser Gly Lys Glu Gly Asp Lys Phe Lys Leu Asn Lys Ser 
            20                  25                  30          


Glu Leu Lys Glu Leu Leu Thr Arg Glu Leu Pro Ser Phe Leu Gly Lys 
        35                  40                  45              


Arg Thr Asp Glu Ala Ala Phe Gln Lys Leu Met Ser Asn Leu Asp Ser 
    50                  55                  60                  


Asn Arg Asp Asn Glu Val Asp Phe Gln Glu Tyr Cys Val Phe Leu Ser 
65                  70                  75                  80  


Cys Ile Ala Met Met Cys Asn Glu Phe Phe Glu Gly Phe Pro Asp Lys 
                85                  90                  95      


Gln Pro Arg Lys Lys 
            100     


<210>  12
<211>  101
<212>  PRT
<213>  Mus musculus

<400>  12

Met Ala Arg Pro Leu Glu Glu Ala Leu Asp Val Ile Val Ser Thr Phe 
1               5                   10                  15      


His Lys Tyr Ser Gly Lys Glu Gly Asp Lys Phe Lys Leu Asn Lys Thr 
            20                  25                  30          


Glu Leu Lys Glu Leu Leu Thr Arg Glu Leu Pro Ser Phe Leu Gly Lys 
        35                  40                  45              


Arg Thr Asp Glu Ala Ala Phe Gln Lys Val Met Ser Asn Leu Asp Ser 
    50                  55                  60                  


Asn Arg Asp Asn Glu Val Asp Phe Gln Glu Tyr Cys Val Phe Leu Ser 
65                  70                  75                  80  


Cys Ile Ala Met Met Cys Asn Glu Phe Phe Glu Gly Cys Pro Asp Lys 
                85                  90                  95      


Glu Pro Arg Lys Lys 
            100     


<210>  13
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  13

Arg Asp Asn Glu Val Asp Phe Gln Glu Tyr Cys Val 
1               5                   10          


<210>  14
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  14

Phe Leu Ser Cys Ile Ala Met Met Cys Asn Glu Phe 
1               5                   10          


<210>  15
<211>  25
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (2)..(2)
<223>  Xaa indicates an amino acid not present in the planar level of 
       the epitope surface and not accessible for the antibody binding

<220>
<221>  SITE
<222>  (4)..(7)
<223>  Xaa indicates an amino acid not present in the planar level of 
       the epitope surface and not accessible for the antibody binding

<220>
<221>  SITE
<222>  (9)..(11)
<223>  Xaa indicates an amino acid not present in the planar level of 
       the epitope surface and not accessible for the antibody binding

<220>
<221>  SITE
<222>  (13)..(15)
<223>  Xaa indicates an amino acid not present in the planar level of 
       the epitope surface and not accessible for the antibody binding

<220>
<221>  SITE
<222>  (18)..(19)
<223>  Xaa indicates an amino acid not present in the planar level of 
       the epitope surface and not accessible for the antibody binding

<220>
<221>  SITE
<222>  (22)..(22)
<223>  Xaa indicates an amino acid not present in the planar level of 
       the epitope surface and not accessible for the antibody binding

<400>  15

Arg Xaa Asn Xaa Xaa Xaa Xaa Gln Xaa Xaa Xaa Val Xaa Xaa Xaa Cys 
1               5                   10                  15      


Ile Xaa Xaa Met Met Xaa Asn Glu Phe 
            20                  25  


<210>  16
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  16

Glu Leu Pro Ser Phe Leu Gly Lys Arg Thr 
1               5                   10  


<210>  17
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  17

Glu Gly Phe Pro Asp Lys Gln Pro Arg Lys Lys 
1               5                   10      


<210>  18
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Ala Cys Pro Leu Glu Lys Ala Leu Asp 
1               5                   10  


<210>  19
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  19

Val Met Val Ser Thr Phe His Lys Tyr Ser 
1               5                   10  


<210>  20
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  20

Gly Lys Glu Gly Asp Lys Phe Lys Leu Asn Lys 
1               5                   10      


<210>  21
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  21

Ser Glu Leu Lys Glu Leu Leu Thr Arg Glu Leu 
1               5                   10      


<210>  22
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  22

Pro Ser Phe Leu Gly Lys Arg Thr Asp Glu Ala 
1               5                   10      


<210>  23
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  23

Ala Phe Gln Lys Leu Met Ser Asn Leu Asp Ser Asn 
1               5                   10          


<210>  24
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  24

Phe Glu Gly Phe Pro Asp Lys Gln Pro Arg Lys Lys 
1               5                   10          


<210>  25
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Mouse Fibronectin forward primer

<400>  25
tgccgcaact actgtgat                                                   18


<210>  26
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Mouse Fibronectin reverse primer

<400>  26
gaatcctggg ctggagta                                                   18


<210>  27
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Mouse G-CSF forward primer

<400>  27
cagatcaccc agaatccat                                                  19


<210>  28
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Mouse G-CSF reverse primer

<400>  28
ctctcgtcct gaccatagtg                                                 20


<210>  29
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Glyceraldehyde 3-phosphate dehydrogenase 
       (GAPDH) forward primer for normalization

<400>  29
tcatccctgc atccactg                                                   18


<210>  30
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: GAPDH reverse primer

<400>  30
taggaacacg gaaggcca                                                   18

