                         SEQUENCE LISTING

<110>  The Board of Regents for Oklahoma State University
 
<120>  USE OF THE SALMONELLA SPP TYPE III SECRETION PROTEINS AS A PROTECTIVE VACCINATION

<130>  13-329 WO

<150>  US 61/716,911
<151>  2012-10-22

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  593
<212>  PRT
<213>  Salmonella enterica

<400>  1

Met Val Asn Asp Ala Ser Ser Ile Ser Arg Ser Gly Tyr Thr Gln Asn 
1               5                   10                  15      


Pro Arg Leu Ala Glu Ala Ala Phe Glu Gly Val Arg Lys Asn Thr Asp 
            20                  25                  30          


Phe Leu Lys Ala Ala Asp Lys Ala Phe Lys Asp Val Val Ala Thr Lys 
        35                  40                  45              


Ala Gly Asp Leu Lys Ala Gly Thr Lys Ser Gly Glu Ser Ala Ile Asn 
    50                  55                  60                  


Thr Val Gly Leu Lys Pro Pro Thr Asp Ala Ala Arg Glu Lys Leu Ser 
65                  70                  75                  80  


Ser Glu Gly Gln Leu Thr Leu Leu Leu Gly Lys Leu Met Thr Leu Leu 
                85                  90                  95      


Gly Asp Val Ser Leu Ser Gln Leu Glu Ser Arg Leu Ala Val Trp Gln 
            100                 105                 110         


Ala Met Ile Glu Ser Gln Lys Glu Met Gly Ile Gln Val Ser Lys Glu 
        115                 120                 125             


Phe Gln Thr Ala Leu Gly Glu Ala Gln Glu Ala Thr Asp Leu Tyr Glu 
    130                 135                 140                 


Ala Ser Ile Lys Lys Thr Asp Thr Ala Lys Ser Val Tyr Asp Ala Ala 
145                 150                 155                 160 


Thr Lys Lys Leu Thr Gln Ala Gln Asn Lys Leu Gln Ser Leu Asp Pro 
                165                 170                 175     


Ala Asp Pro Gly Tyr Ala Gln Ala Glu Ala Ala Val Glu Gln Ala Gly 
            180                 185                 190         


Lys Glu Ala Thr Glu Ala Lys Glu Ala Leu Asp Lys Ala Thr Asp Ala 
        195                 200                 205             


Thr Val Lys Ala Gly Thr Asp Ala Lys Ala Lys Ala Glu Lys Ala Asp 
    210                 215                 220                 


Asn Ile Leu Thr Lys Phe Gln Gly Thr Ala Asn Ala Ala Ser Gln Asn 
225                 230                 235                 240 


Gln Val Ser Gln Gly Glu Gln Asp Asn Leu Ser Asn Val Ala Arg Leu 
                245                 250                 255     


Thr Met Leu Met Ala Met Phe Ile Glu Ile Val Gly Lys Asn Thr Glu 
            260                 265                 270         


Glu Ser Leu Gln Asn Asp Leu Ala Leu Phe Asn Ala Leu Gln Glu Gly 
        275                 280                 285             


Arg Gln Ala Glu Met Glu Lys Lys Ser Ala Glu Phe Gln Glu Glu Thr 
    290                 295                 300                 


Arg Lys Ala Glu Glu Thr Asn Arg Ile Met Gly Cys Ile Gly Lys Val 
305                 310                 315                 320 


Leu Gly Ala Leu Leu Thr Ile Val Ser Val Val Ala Ala Val Phe Thr 
                325                 330                 335     


Gly Gly Ala Ser Leu Ala Leu Ala Ala Val Gly Leu Ala Val Met Val 
            340                 345                 350         


Ala Asp Glu Ile Val Lys Ala Ala Thr Gly Val Ser Phe Ile Gln Gln 
        355                 360                 365             


Ala Leu Asn Pro Ile Met Glu His Val Leu Lys Pro Leu Met Glu Leu 
    370                 375                 380                 


Ile Gly Lys Ala Ile Thr Lys Ala Leu Glu Gly Leu Gly Val Asp Lys 
385                 390                 395                 400 


Lys Thr Ala Glu Met Ala Gly Ser Ile Val Gly Ala Ile Val Ala Ala 
                405                 410                 415     


Ile Ala Met Val Ala Val Ile Val Val Val Ala Val Val Gly Lys Gly 
            420                 425                 430         


Ala Ala Ala Lys Leu Gly Asn Ala Leu Ser Lys Met Met Gly Glu Thr 
        435                 440                 445             


Ile Lys Lys Leu Val Pro Asn Val Leu Lys Gln Leu Ala Gln Asn Gly 
    450                 455                 460                 


Ser Lys Leu Phe Thr Gln Gly Met Gln Arg Ile Thr Ser Gly Leu Gly 
465                 470                 475                 480 


Asn Val Gly Ser Lys Met Gly Leu Gln Thr Asn Ala Leu Ser Lys Glu 
                485                 490                 495     


Leu Val Gly Asn Thr Leu Asn Lys Val Ala Leu Gly Met Glu Val Thr 
            500                 505                 510         


Asn Thr Ala Ala Gln Ser Ala Gly Gly Val Ala Glu Gly Val Phe Ile 
        515                 520                 525             


Lys Asn Ala Ser Glu Ala Leu Ala Asp Phe Met Leu Ala Arg Phe Ala 
    530                 535                 540                 


Met Asp Gln Ile Gln Gln Trp Leu Lys Gln Ser Val Glu Ile Phe Gly 
545                 550                 555                 560 


Glu Asn Gln Lys Val Thr Ala Glu Leu Gln Lys Ala Met Ser Ser Ala 
                565                 570                 575     


Val Gln Gln Asn Ala Asp Ala Ser Arg Phe Ile Leu Arg Gln Ser Arg 
            580                 585                 590         


Ala 
    


<210>  2
<211>  1782
<212>  DNA
<213>  Salmonella enterica

<400>  2
ttatgcgcga ctctggcgca gaataaaacg cgaagcatcc gcattttgct gtaccgcaga       60

agacatggct ttttgcagtt ccgccgttac cttctggttt tcaccaaata tttctacgga      120

ttgtttaagc cactgctgaa tctgatccat ggcaaaacgg gcgagcataa aatcagcaag      180

cgcctcgctg gcatttttaa taaatacgcc ctcggcaaca ccaccggctg actgggctgc      240

ggtattcgtg acttccatgc ccaacgccac tttatttagg gtattaccta ccagctcttt      300

acttaaggca ttcgtttgca ggcccatctt gctacccaca ttacccagac cgctagtaat      360

acgttgcatc ccctgggtaa agagtttgct gccgttttgc gccaactgtt tcagcacgtt      420

aggcaccaac ttcttaatcg tttcgcccat cattttgctc agcgcgttac ccagtttcgc      480

cgccgcgcct ttcccgacaa ctgcgaccac cacaatgacc gccaccatgg caatagcggc      540

gacaatcgca ccaacaatgc tgccggccat ctctgccgtt ttcttatcga cgcctaatcc      600

ttccagcgct ttggtaatcg ccttgccaat cagctccatt aacggcttca gcacatgctc      660

cataatcggg tttagcgcct gctgaataaa cgacactccc gtcgccgcct tcacaatttc      720

atcggccacc attaccgcaa gtcccaccgc agccagcgcc agactcgccc caccggtaaa      780

aacagcggcc acaacgctga caatggttag cagcgcgccg aggactttcc cgatacatcc      840

cataatgcgg ttcgtttcct cggctttgcg cgtctcttcc tggaattcag ccgatttctt      900

ttccatctcc gcctgacgcc cttcctgcaa ggcgttgaaa agcgcaagat cgttttgcag      960

gctttcttcc gtatttttgc ccacaatctc aataaacatg gccatgagca tagtgaggcg     1020

ggcgacattt gacagattat cctgctcacc ctgggaaacc tgattctgag aggcggcatt     1080

agccgttccc tggaatttgg tcagaatgtt atccgctttc tcggctttcg ctttggcgtc     1140

tgtgcctgct ttaaccgtcg catccgtggc cttatctaag gcctctttcg cctctgtcgc     1200

ttcttttccg gcctgttcta ccgcggcttc agcttgtgca tagccggggt cagccgggtc     1260

cagcgattgc aatttatttt gcgcctgcgt cagttttttg gtcgcagcgt cataaacact     1320

cttggcggta tccgtctttt tgatactggc ttcatagaga tccgtcgcct cctgagcctc     1380

tcccagagcc gtctggaatt ctttcgatac ctgaatcccc atctcttttt gtgactcaat     1440

catcgcctgc cataccgcca gacgagactc cagttgagac agcgaaacat cgcccagtag     1500

ggtcattaac ttgccaagca gtaatgtcaa ttgcccttcg ctggagagtt tttcccgggc     1560

ggcgtccgta ggcggcttta gacccaccgt attaatagcg ctctcgccgg actttgttcc     1620

ggctttaagg tcgcccgctt tcgttgccac cacatcttta aaagctttat ccgccgcttt     1680

taaaaagtcc gtgttcttac gaacgccttc aaaagccgcc tcagcgaggc gcggattttg     1740

ggtatatccg ctacggctaa tgctacttgc gtcatttacc at                        1782


<210>  3
<211>  339
<212>  PRT
<213>  Salmonella enterica

<400>  3

Met Leu Asn Ile Gln Asn Tyr Ser Ala Ser Pro His Pro Gly Ile Val 
1               5                   10                  15      


Ala Glu Arg Pro Gln Thr Pro Ser Ala Ser Glu His Ala Glu Ile Ala 
            20                  25                  30          


Val Val Pro Ser Thr Thr Glu His Arg Gly Thr Asp Ile Ile Ser Leu 
        35                  40                  45              


Ser Gln Ala Ala Thr Lys Ile Gln Gln Ala Gln Gln Thr Leu Gln Ser 
    50                  55                  60                  


Thr Pro Pro Ile Ser Glu Glu Asn Asn Asp Glu Arg Thr Leu Ala Arg 
65                  70                  75                  80  


Gln Gln Leu Thr Ser Ser Leu Asn Ala Leu Ala Lys Ser Gly Val Ser 
                85                  90                  95      


Leu Ser Ala Glu Gln Asn Glu Asn Leu Arg Ser Thr Phe Ser Ala Arg 
            100                 105                 110         


Arg Arg Pro Tyr Leu Ala Leu Arg Leu Trp Pro Ala Arg Thr Thr Ile 
        115                 120                 125             


Ser Asp Ala Glu Ile Trp Asp Met Val Ser Gln Asn Ile Ser Ala Ile 
    130                 135                 140                 


Gly Asp Ser Tyr Leu Gly Val Tyr Glu Asn Val Val Ala Val Tyr Thr 
145                 150                 155                 160 


Asp Phe Tyr Gln Ala Phe Ser Asp Ile Leu Ser Lys Met Gly Gly Trp 
                165                 170                 175     


Leu Ser Pro Gly Lys Asp Gly Asn Thr Ile Lys Leu Asn Val Asp Ser 
            180                 185                 190         


Leu Lys Ser Glu Ile Ser Ser Leu Ile Asn Lys Tyr Thr Gln Ile Asn 
        195                 200                 205             


Lys Asn Thr Ile Leu Phe Pro Ser Gln Thr Gly Ser Gly Met Thr Thr 
    210                 215                 220                 


Ala Thr Lys Ala Glu Ala Glu Gln Trp Ile Lys Glu Leu Asn Leu Pro 
225                 230                 235                 240 


Asp Ser Cys Leu Lys Ala Ser Gly Ser Gly Tyr Val Val Leu Val Asp 
                245                 250                 255     


Thr Gly Pro Leu Ser Lys Met Val Ser Asp Leu Asn Gly Ile Gly Ser 
            260                 265                 270         


Gly Ser Ala Leu Glu Leu Asp Asn Ala Lys Tyr Gln Ala Trp Gln Ser 
        275                 280                 285             


Gly Phe Lys Ala Gln Glu Glu Asn Leu Lys Thr Thr Leu Gln Thr Leu 
    290                 295                 300                 


Thr Gln Lys Tyr Ser Asn Ala Asn Ser Leu Tyr Asp Asn Leu Val Lys 
305                 310                 315                 320 


Val Leu Ser Ser Thr Ile Ser Ser Ser Leu Glu Thr Ala Lys Ser Phe 
                325                 330                 335     


Leu Gln Gly 
            


<210>  4
<211>  1020
<212>  DNA
<213>  Salmonella enterica

<400>  4
atgcttaata ttcaaaatta ttccgcttct cctcatccgg ggatcgttgc cgaacggccg       60

cagactcctt cggcgagcga gcacgccgag attgccgtgg taccgtctac cacagaacat      120

cgcggcacag atatcatttc attatcgcag gcggctacta aaatccagca ggcacagcag      180

acgctgcagt caacgccacc gatttctgaa gagaataatg acgagcgcac gctggcgcgc      240

caacagttga ccagcagcct gaatgcgctg gcgaagtccg gcgtgtcatt atccgcagaa      300

caaaatgaga acctgcggag cacgttttct gcgcgacgtc ggccttattt agcgcttcgc      360

ctatggccag cgagaacaac catttctgat gctgagattt gggatatggt ttcccaaaat      420

atatcggcga taggtgacag ctacctgggc gtttatgaaa acgttgtcgc agtctatacc      480

gatttttatc aggccttcag tgatattctt tccaaaatgg gaggctggtt atcgcctggt      540

aaggatggaa ataccattaa gctaaatgtt gactcactta aaagtgaaat aagtagttta      600

attaataaat acactcaaat aaataaaaat acgattttat ttccctcgca aactggcagc      660

ggaatgacaa cagcaacgaa agcggaagct gagcagtgga ttaaagaatt gaatttaccg      720

gacagctgtc taaaggcgtc tggttctggt tatgtcgtac tggtggatac ggggccactg      780

agcaaaatgg ttagcgatct taatggaata ggatcgggtt cagcccttga actggataac      840

gccaaatatc aagcctggca gtcgggtttt aaagcacagg aagaaaatct gaaaaccaca      900

ttacagacgc tgacgcaaaa atatagcaat gccaattcat tgtacgacaa cctggtaaaa      960

gtgctgagca gtacgataag tagcagcctg gaaaccgcca aaagcttcct gcaaggataa     1020


<210>  5
<211>  196
<212>  PRT
<213>  Salmonella enterica

<400>  5

Met Ser Ser Gly Asn Ile Leu Trp Gly Ser Gln Asn Pro Ile Val Phe 
1               5                   10                  15      


Lys Asn Ser Phe Gly Val Ser Asn Ala Asp Thr Gly Ser Gln Asp Asp 
            20                  25                  30          


Leu Ser Gln Gln Asn Pro Phe Ala Glu Gly Tyr Gly Val Leu Leu Ile 
        35                  40                  45              


Leu Leu Met Val Ile Gln Ala Ile Ala Asn Asn Lys Phe Ile Glu Val 
    50                  55                  60                  


Gln Lys Asn Ala Glu Arg Ala Arg Asn Thr Gln Glu Lys Ser Asn Glu 
65                  70                  75                  80  


Met Asp Glu Val Ile Ala Lys Ala Ala Lys Gly Asp Ala Lys Thr Lys 
                85                  90                  95      


Glu Glu Val Pro Glu Asp Val Ile Lys Tyr Met Arg Asp Asn Gly Ile 
            100                 105                 110         


Leu Ile Asp Gly Met Thr Ile Asp Asp Tyr Met Ala Lys Tyr Gly Asp 
        115                 120                 125             


His Gly Lys Leu Asp Lys Gly Gly Leu Gln Ala Ile Lys Ala Ala Leu 
    130                 135                 140                 


Asp Asn Asp Ala Asn Arg Asn Thr Asp Leu Met Ser Gln Gly Gln Ile 
145                 150                 155                 160 


Thr Ile Gln Lys Met Ser Gln Glu Leu Asn Ala Val Leu Thr Gln Leu 
                165                 170                 175     


Thr Gly Leu Ile Ser Lys Trp Gly Glu Ile Ser Ser Met Ile Ala Gln 
            180                 185                 190         


Lys Thr Tyr Ser 
        195     


<210>  6
<211>  591
<212>  DNA
<213>  Salmonella enterica

<400>  6
atgtcttcag gaaacatctt atggggaagt caaaacccta ttgtgtttaa aaatagcttc       60

ggcgtcagca acgctgatac cgggagccag gatgacttat cccagcaaaa tccgtttgcc      120

gaagggtatg gtgttttgct tattctcctt atggttattc aggctatcgc aaataataaa      180

tttattgaag tccagaagaa cgctgaacgt gccagaaata cccaggaaaa gtcaaatgag      240

atggatgagg tgattgctaa agcagccaaa ggggatgcta aaaccaaaga ggaggtgcct      300

gaggatgtaa ttaaatacat gcgtgataat ggtattctca tcgatggtat gaccattgat      360

gattatatgg ctaaatatgg cgatcatggg aagctggata aaggtggcct acaggcgatc      420

aaagcggctt tggataatga cgccaaccgg aataccgatc ttatgagtca ggggcagata      480

acaattcaaa aaatgtctca ggagcttaac gctgtcctta cccaactgac agggcttatc      540

agtaagtggg gggaaatttc cagtatgata gcgcagaaaa cgtactcatg a               591


<210>  7
<211>  934
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic SipB-SipD chimera

<400>  7

Met Leu Asn Ile Gln Asn Tyr Ser Ala Ser Pro His Pro Gly Ile Val 
1               5                   10                  15      


Ala Glu Arg Pro Gln Thr Pro Ser Ala Ser Glu His Ala Glu Ile Ala 
            20                  25                  30          


Val Val Pro Ser Thr Thr Glu His Arg Gly Thr Asp Ile Ile Ser Leu 
        35                  40                  45              


Ser Gln Ala Ala Thr Lys Ile Gln Gln Ala Gln Gln Thr Leu Gln Ser 
    50                  55                  60                  


Thr Pro Pro Ile Ser Glu Glu Asn Asn Asp Glu Arg Thr Leu Ala Arg 
65                  70                  75                  80  


Gln Gln Leu Thr Ser Ser Leu Asn Ala Leu Ala Lys Ser Gly Val Ser 
                85                  90                  95      


Leu Ser Ala Glu Gln Asn Glu Asn Leu Arg Ser Thr Phe Ser Ala Arg 
            100                 105                 110         


Arg Arg Pro Tyr Leu Ala Leu Arg Leu Trp Pro Ala Arg Thr Thr Ile 
        115                 120                 125             


Ser Asp Ala Glu Ile Trp Asp Met Val Ser Gln Asn Ile Ser Ala Ile 
    130                 135                 140                 


Gly Asp Ser Tyr Leu Gly Val Tyr Glu Asn Val Val Ala Val Tyr Thr 
145                 150                 155                 160 


Asp Phe Tyr Gln Ala Phe Ser Asp Ile Leu Ser Lys Met Gly Gly Trp 
                165                 170                 175     


Leu Ser Pro Gly Lys Asp Gly Asn Thr Ile Lys Leu Asn Val Asp Ser 
            180                 185                 190         


Leu Lys Ser Glu Ile Ser Ser Leu Ile Asn Lys Tyr Thr Gln Ile Asn 
        195                 200                 205             


Lys Asn Thr Ile Leu Phe Pro Ser Gln Thr Gly Ser Gly Met Thr Thr 
    210                 215                 220                 


Ala Thr Lys Ala Glu Ala Glu Gln Trp Ile Lys Glu Leu Asn Leu Pro 
225                 230                 235                 240 


Asp Ser Cys Leu Lys Ala Ser Gly Ser Gly Tyr Val Val Leu Val Asp 
                245                 250                 255     


Thr Gly Pro Leu Ser Lys Met Val Ser Asp Leu Asn Gly Ile Gly Ser 
            260                 265                 270         


Gly Ser Ala Leu Glu Leu Asp Asn Ala Lys Tyr Gln Ala Trp Gln Ser 
        275                 280                 285             


Gly Phe Lys Ala Gln Glu Glu Asn Leu Lys Thr Thr Leu Gln Thr Leu 
    290                 295                 300                 


Thr Gln Lys Tyr Ser Asn Ala Asn Ser Leu Tyr Asp Asn Leu Val Lys 
305                 310                 315                 320 


Val Leu Ser Ser Thr Ile Ser Ser Ser Leu Glu Thr Ala Lys Ser Phe 
                325                 330                 335     


Leu Gln Gly Val Asp Met Val Asn Asp Ala Ser Ser Ile Ser Arg Ser 
            340                 345                 350         


Gly Tyr Thr Gln Asn Pro Arg Leu Ala Glu Ala Ala Phe Glu Gly Val 
        355                 360                 365             


Arg Lys Asn Thr Asp Phe Leu Lys Ala Ala Asp Lys Ala Phe Lys Asp 
    370                 375                 380                 


Val Val Ala Thr Lys Ala Gly Asp Leu Lys Ala Gly Thr Lys Ser Gly 
385                 390                 395                 400 


Glu Ser Ala Ile Asn Thr Val Gly Leu Lys Pro Pro Thr Asp Ala Ala 
                405                 410                 415     


Arg Glu Lys Leu Ser Ser Glu Gly Gln Leu Thr Leu Leu Leu Gly Lys 
            420                 425                 430         


Leu Met Thr Leu Leu Gly Asp Val Ser Leu Ser Gln Leu Glu Ser Arg 
        435                 440                 445             


Leu Ala Val Trp Gln Ala Met Ile Glu Ser Gln Lys Glu Met Gly Ile 
    450                 455                 460                 


Gln Val Ser Lys Glu Phe Gln Thr Ala Leu Gly Glu Ala Gln Glu Ala 
465                 470                 475                 480 


Thr Asp Leu Tyr Glu Ala Ser Ile Lys Lys Thr Asp Thr Ala Lys Ser 
                485                 490                 495     


Val Tyr Asp Ala Ala Thr Lys Lys Leu Thr Gln Ala Gln Asn Lys Leu 
            500                 505                 510         


Gln Ser Leu Asp Pro Ala Asp Pro Gly Tyr Ala Gln Ala Glu Ala Ala 
        515                 520                 525             


Val Glu Gln Ala Gly Lys Glu Ala Thr Glu Ala Lys Glu Ala Leu Asp 
    530                 535                 540                 


Lys Ala Thr Asp Ala Thr Val Lys Ala Gly Thr Asp Ala Lys Ala Lys 
545                 550                 555                 560 


Ala Glu Lys Ala Asp Asn Ile Leu Thr Lys Phe Gln Gly Thr Ala Asn 
                565                 570                 575     


Ala Ala Ser Gln Asn Gln Val Ser Gln Gly Glu Gln Asp Asn Leu Ser 
            580                 585                 590         


Asn Val Ala Arg Leu Thr Met Leu Met Ala Met Phe Ile Glu Ile Val 
        595                 600                 605             


Gly Lys Asn Thr Glu Glu Ser Leu Gln Asn Asp Leu Ala Leu Phe Asn 
    610                 615                 620                 


Ala Leu Gln Glu Gly Arg Gln Ala Glu Met Glu Lys Lys Ser Ala Glu 
625                 630                 635                 640 


Phe Gln Glu Glu Thr Arg Lys Ala Glu Glu Thr Asn Arg Ile Met Gly 
                645                 650                 655     


Cys Ile Gly Lys Val Leu Gly Ala Leu Leu Thr Ile Val Ser Val Val 
            660                 665                 670         


Ala Ala Val Phe Thr Gly Gly Ala Ser Leu Ala Leu Ala Ala Val Gly 
        675                 680                 685             


Leu Ala Val Met Val Ala Asp Glu Ile Val Lys Ala Ala Thr Gly Val 
    690                 695                 700                 


Ser Phe Ile Gln Gln Ala Leu Asn Pro Ile Met Glu His Val Leu Lys 
705                 710                 715                 720 


Pro Leu Met Glu Leu Ile Gly Lys Ala Ile Thr Lys Ala Leu Glu Gly 
                725                 730                 735     


Leu Gly Val Asp Lys Lys Thr Ala Glu Met Ala Gly Ser Ile Val Gly 
            740                 745                 750         


Ala Ile Val Ala Ala Ile Ala Met Val Ala Val Ile Val Val Val Ala 
        755                 760                 765             


Val Val Gly Lys Gly Ala Ala Ala Lys Leu Gly Asn Ala Leu Ser Lys 
    770                 775                 780                 


Met Met Gly Glu Thr Ile Lys Lys Leu Val Pro Asn Val Leu Lys Gln 
785                 790                 795                 800 


Leu Ala Gln Asn Gly Ser Lys Leu Phe Thr Gln Gly Met Gln Arg Ile 
                805                 810                 815     


Thr Ser Gly Leu Gly Asn Val Gly Ser Lys Met Gly Leu Gln Thr Asn 
            820                 825                 830         


Ala Leu Ser Lys Glu Leu Val Gly Asn Thr Leu Asn Lys Val Ala Leu 
        835                 840                 845             


Gly Met Glu Val Thr Asn Thr Ala Ala Gln Ser Ala Gly Gly Val Ala 
    850                 855                 860                 


Glu Gly Val Phe Ile Lys Asn Ala Ser Glu Ala Leu Ala Asp Phe Met 
865                 870                 875                 880 


Leu Ala Arg Phe Ala Met Asp Gln Ile Gln Gln Trp Leu Lys Gln Ser 
                885                 890                 895     


Val Glu Ile Phe Gly Glu Asn Gln Lys Val Thr Ala Glu Leu Gln Lys 
            900                 905                 910         


Ala Met Ser Ser Ala Val Gln Gln Asn Ala Asp Ala Ser Arg Phe Ile 
        915                 920                 925             


Leu Arg Gln Ser Arg Ala 
    930                 


<210>  8
<211>  2805
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleic acid sequence encoding SipB-SipD chimera

<400>  8
atgcttaata ttcaaaatta ttccgcttct cctcatccgg ggatcgttgc cgaacggccg       60

cagactcctt cggcgagcga gcacgccgag attgccgtgg taccgtctac cacagaacat      120

cgcggcacag atatcatttc attatcgcag gcggctacta aaatccagca ggcacagcag      180

acgctgcagt caacgccacc gatttctgaa gagaataatg acgagcgcac gctggcgcgc      240

caacagttga ccagcagcct gaatgcgctg gcgaagtccg gcgtgtcatt atccgcagaa      300

caaaatgaga acctgcggag cacgttttct gcgcgacgtc ggccttattt agcgcttcgc      360

ctatggccag cgagaacaac catttctgat gctgagattt gggatatggt ttcccaaaat      420

atatcggcga taggtgacag ctacctgggc gtttatgaaa acgttgtcgc agtctatacc      480

gatttttatc aggccttcag tgatattctt tccaaaatgg gaggctggtt atcgcctggt      540

aaggatggaa ataccattaa gctaaatgtt gactcactta aaagtgaaat aagtagttta      600

attaataaat acactcaaat aaataaaaat acgattttat ttccctcgca aactggcagc      660

ggaatgacaa cagcaacgaa agcggaagct gagcagtgga ttaaagaatt gaatttaccg      720

gacagctgtc taaaggcgtc tggttctggt tatgtcgtac tggtggatac ggggccactg      780

agcaaaatgg ttagcgatct taatggaata ggatcgggtt cagcccttga actggataac      840

gccaaatatc aagcctggca gtcgggtttt aaagcacagg aagaaaatct gaaaaccaca      900

ttacagacgc tgacgcaaaa atatagcaat gccaattcat tgtacgacaa cctggtaaaa      960

gtgctgagca gtacgataag tagcagcctg gaaaccgcca aaagcttcct gcaaggagtc     1020

gacttatgcg cgactctggc gcagaataaa acgcgaagca tccgcatttt gctgtaccgc     1080

agaagacatg gctttttgca gttccgccgt taccttctgg ttttcaccaa atatttctac     1140

ggattgttta agccactgct gaatctgatc catggcaaaa cgggcgagca taaaatcagc     1200

aagcgcctcg ctggcatttt taataaatac gccctcggca acaccaccgg ctgactgggc     1260

tgcggtattc gtgacttcca tgcccaacgc cactttattt agggtattac ctaccagctc     1320

tttacttaag gcattcgttt gcaggcccat cttgctaccc acattaccca gaccgctagt     1380

aatacgttgc atcccctggg taaagagttt gctgccgttt tgcgccaact gtttcagcac     1440

gttaggcacc aacttcttaa tcgtttcgcc catcattttg ctcagcgcgt tacccagttt     1500

cgccgccgcg cctttcccga caactgcgac caccacaatg accgccacca tggcaatagc     1560

ggcgacaatc gcaccaacaa tgctgccggc catctctgcc gttttcttat cgacgcctaa     1620

tccttccagc gctttggtaa tcgccttgcc aatcagctcc attaacggct tcagcacatg     1680

ctccataatc gggtttagcg cctgctgaat aaacgacact cccgtcgccg ccttcacaat     1740

ttcatcggcc accattaccg caagtcccac cgcagccagc gccagactcg ccccaccggt     1800

aaaaacagcg gccacaacgc tgacaatggt tagcagcgcg ccgaggactt tcccgataca     1860

tcccataatg cggttcgttt cctcggcttt gcgcgtctct tcctggaatt cagccgattt     1920

cttttccatc tccgcctgac gcccttcctg caaggcgttg aaaagcgcaa gatcgttttg     1980

caggctttct tccgtatttt tgcccacaat ctcaataaac atggccatga gcatagtgag     2040

gcgggcgaca tttgacagat tatcctgctc accctgggaa acctgattct gagaggcggc     2100

attagccgtt ccctggaatt tggtcagaat gttatccgct ttctcggctt tcgctttggc     2160

gtctgtgcct gctttaaccg tcgcatccgt ggccttatct aaggcctctt tcgcctctgt     2220

cgcttctttt ccggcctgtt ctaccgcggc ttcagcttgt gcatagccgg ggtcagccgg     2280

gtccagcgat tgcaatttat tttgcgcctg cgtcagtttt ttggtcgcag cgtcataaac     2340

actcttggcg gtatccgtct ttttgatact ggcttcatag agatccgtcg cctcctgagc     2400

ctctcccaga gccgtctgga attctttcga tacctgaatc cccatctctt tttgtgactc     2460

aatcatcgcc tgccataccg ccagacgaga ctccagttga gacagcgaaa catcgcccag     2520

tagggtcatt aacttgccaa gcagtaatgt caattgccct tcgctggaga gtttttcccg     2580

ggcggcgtcc gtaggcggct ttagacccac cgtattaata gcgctctcgc cggactttgt     2640

tccggcttta aggtcgcccg ctttcgttgc caccacatct ttaaaagctt tatccgccgc     2700

ttttaaaaag tccgtgttct tacgaacgcc ttcaaaagcc gcctcagcga ggcgcggatt     2760

ttgggtatat ccgctacggc taatgctact tgcgtcattt accat                     2805


