                         SEQUENCE LISTING

<110>  OXFORD NANOPORE TECHNOLOGIES LIMITED
 
<120>  Formation of Array of Membranes and Apparatus Therefor

<130>  N117579B-WO

<140>  tba
<141>  2013-10-23

<150>  GB1313121.4
<151>  2013-07-23

<150>  US61/718,899
<151>  2012-10-26

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MspA-B2C

<400>  1

Met Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr 
1               5                   10                  15      


Leu Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu 
            20                  25                  30          


Asp Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys 
        35                  40                  45              


Tyr Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu 
    50                  55                  60                  


Leu Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Ser Val Ser Ile Asn 
65                  70                  75                  80  


Phe Ser Tyr Thr Thr Pro Asn Ile Asn Ile Asn Asn Gly Asn Ile Thr 
                85                  90                  95      


Ala Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro 
            100                 105                 110         


Gly Val Ser Ile Ser Ala Arg Leu Gly Asn Gly Pro Gly Ile Arg Glu 
        115                 120                 125             


Val Ala Thr Phe Ser Val Arg Val Ser Gly Ala Lys Gly Gly Val Ala 
    130                 135                 140                 


Val Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu 
145                 150                 155                 160 


Leu Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr 
                165                 170                 175     


Thr Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 185 


<210>  2
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MS-B1 mutant

<400>  2

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asn Asn Gly Asn Ile Thr Ala 
                85                  90                  95      


Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Arg Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Arg Val Ser Gly Ala Lys Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  3
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide sequence used in Example 5

<400>  3
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt ggttgtttct       60

gttggtgctg atattgc                                                      77


<210>  4
<211>  530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide sequence used in Example 5

<400>  4
gacgctcagt aatgtgacga tagctgaaaa ctgtacgata aacggtacgc tgagggcgga       60

aaaaatcgtc ggggacattg taaaggcggc gagcgcggct tttccgcgcc agcgtgaaag      120

cagtgtggac tggccgtcag gtacccgtac tgtcaccgtg accgatgacc atccttttga      180

tcgccagata gtggtgcttc cgctgacgtt tcgcggaagt aagcgtactg tcagcggcag      240

gacaacgtat tcgatgtgtt atctgaaagt actgatgaac ggtgcggtga tttatgatgg      300

cgcggcgaac gaggcggtac aggtgttctc ccgtattgtt gacatgccag cgggtcgggg      360

aaacgtgatc ctgacgttca cgcttacgtc cacacggcat tcggcagata ttccgccgta      420

tacgtttgcc agcgatgtgc aggttatggt gattaagaaa caggcgctgg gcatcagcgt      480

ggtctgagtg tgaaaaaaaa aaccccaaaa aaaaaacccc aaaaaaaaaa                 530


<210>  5
<211>  885
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide encoding one subunit of 
       alpha-hemolysin-E111N/K147N

<400>  5
atggcagatt ctgatattaa tattaaaacc ggtactacag atattggaag caatactaca       60

gtaaaaacag gtgatttagt cacttatgat aaagaaaatg gcatgcacaa aaaagtattt      120

tatagtttta tcgatgataa aaatcacaat aaaaaactgc tagttattag aacaaaaggt      180

accattgctg gtcaatatag agtttatagc gaagaaggtg ctaacaaaag tggtttagcc      240

tggccttcag cctttaaggt acagttgcaa ctacctgata atgaagtagc tcaaatatct      300

gattactatc caagaaattc gattgataca aaaaactata tgagtacttt aacttatgga      360

ttcaacggta atgttactgg tgatgataca ggaaaaattg gcggccttat tggtgcaaat      420

gtttcgattg gtcatacact gaactatgtt caacctgatt tcaaaacaat tttagagagc      480

ccaactgata aaaaagtagg ctggaaagtg atatttaaca atatggtgaa tcaaaattgg      540

ggaccatacg atcgagattc ttggaacccg gtatatggca atcaactttt catgaaaact      600

agaaatggtt ctatgaaagc agcagataac ttccttgatc ctaacaaagc aagttctcta      660

ttatcttcag ggttttcacc agacttcgct acagttatta ctatggatag aaaagcatcc      720

aaacaacaaa caaatataga tgtaatatac gaacgagttc gtgatgatta ccaattgcat      780

tggacttcaa caaattggaa aggtaccaat actaaagata aatggacaga tcgttcttca      840

gaaagatata aaatcgattg ggaaaaagaa gaaatgacaa attaa                      885


<210>  6
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of one subunit of alpha-hemolysin-E111N/K147N

<400>  6

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Asn Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Asn Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  7 

<400>  7

000

<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelled polynucleotide strand


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  BHQ1 label

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  FAM label

<400>  8
tttttttttt tttttttttt tttttttttt                                        30


<210>  9
<211>  555
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide sequence encoding the MspA-(B2C) mutant MspA 
       monomer

<400>  9
atgggtctgg ataatgaact gagcctggtg gacggtcaag atcgtaccct gacggtgcaa       60

caatgggata cctttctgaa tggcgttttt ccgctggatc gtaatcgcct gacccgtgaa      120

tggtttcatt ccggtcgcgc aaaatatatc gtcgcaggcc cgggtgctga cgaattcgaa      180

ggcacgctgg aactgggtta tcagattggc tttccgtggt cactgagcgt tagtatcaac      240

ttctcgtaca ccacgccgaa tattaacatc aacaatggta acattaccgc accgccgttt      300

ggcctgaaca gcgtgattac gccgaacctg tttccgggtg ttagcatctc tgcccgtctg      360

ggcaatggtc cgggcattcg cgaagtggca acctttagtg tgcgcgtttc cggcgctaaa      420

ggcggtgtcg cggtgtctaa cgcccacggt accgttacgg gcgcggccgg cggtgtcctg      480

ctgcgtccgt tcgcgcgcct gattgcctct accggcgaca gcgttacgac ctatggcgaa      540

ccgtggaata tgaac                                                       555


<210>  10
<211>  558
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide sequence encoding the MS-B1 mutant of the MspA 
       monomer

<400>  10
atgggtctgg ataatgaact gagcctggtg gacggtcaag atcgtaccct gacggtgcaa       60

caatgggata cctttctgaa tggcgttttt ccgctggatc gtaatcgcct gacccgtgaa      120

tggtttcatt ccggtcgcgc aaaatatatc gtcgcaggcc cgggtgctga cgaattcgaa      180

ggcacgctgg aactgggtta tcagattggc tttccgtggt cactgggcgt tggtatcaac      240

ttctcgtaca ccacgccgaa tattctgatc aacaatggta acattaccgc accgccgttt      300

ggcctgaaca gcgtgattac gccgaacctg tttccgggtg ttagcatctc tgcccgtctg      360

ggcaatggtc cgggcattca agaagtggca acctttagtg tgcgcgtttc cggcgctaaa      420

ggcggtgtcg cggtgtctaa cgcccacggt accgttacgg gcgcggccgg cggtgtcctg      480

ctgcgtccgt tcgcgcgcct gattgcctct accggcgaca gcgttacgac ctatggcgaa      540

ccgtggaata tgaactaa                                                    558


