                         SEQUENCE LISTING

<110>  Vrije Universiteit Brussel
 
<120>  Vectors for liver-directed gene therapy of hemophilia and methods
        and use thereof

<130>  VUB-057-PCT2

<150>  PCT/EP2012/071297
<151>  2012-10-26

<150>  PCT/EP2013/064054
<151>  2013-07-03

<160>  30    

<170>  PatentIn version 3.3

<210>  1
<211>  6044
<212>  DNA
<213>  Artificial

<220>
<223>  Adenoviral expression vector pdsAAVsc SerpEnh TTRmin MVM 
       FIXcoptMT-bghpA

<400>  1
cagcagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag     60

cctgaatggc gaatggaatt ccagacgatt gagcgtcaaa atgtaggtat ttccatgagc    120

gtttttcctg ttgcaatggc tggcggtaat attgttctgg atattaccag caaggccgat    180

agtttgagtt cttctactca ggcaagtgat gttattacta atcaaagaag tattgcgaca    240

acggttaatt tgcgtgatgg acagactctt ttactcggtg gcctcactga ttataaaaac    300

acttctcagg attctggcgt accgttcctg tctaaaatcc ctttaatcgg cctcctgttt    360

agctcccgct ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa agcaaccata    420

gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac    480

cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc    540

cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt    600

tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg    660

gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag    720

tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt    780

ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt    840

taacgcgaat tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt    900

cctgtttttg gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt    960

acgattaccg ttcatcgcct gcactgcgcg ctcgctcgct cactgaggcc gcccgggcaa   1020

agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag   1080

agggagtgga attcacgcgt ggtacgatct gaattcggta caattcacgc gtggtacggc   1140

cgcggtaccg gcgcgccggg ggaggctgct ggtgaatatt aaccaaggtc accccagtta   1200

tcggaggagc aaacaggggc taagtccaca cgcgtggtac cgtctgtctg cacatttcgt   1260

agagcgagtg ttccgatact ctaatctccc taggcaaggt tcatatttgt gtaggttact   1320

tattctcctt ttgttgacta agtcaataat cagaatcagc aggtttggag tcagcttggc   1380

agggatcagc agcctgggtt ggaaggaggg ggtataaaag ccccttcacc aggagaagcc   1440

gtcacacaga tccacaagct cctgaagagg taagggttta agggatggtt ggttggtggg   1500

gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggctag   1560

catgcagcgc gtgaacatga tcatggccga gagccccggc ctgatcacca tctgcctgct   1620

gggctacctg ctgagcgccg agtgcaccgt gttcctggac cacgagaacg ccaacaagat   1680

cctgaaccgc cccaagcgct acaacagcgg caagctggag gagttcgtgc agggcaacct   1740

ggagcgcgag tgcatggagg agaagtgcag cttcgaggag gcccgcgagg tgttcgagaa   1800

caccgagcgc accaccgagt tctggaagca gtacgtggac ggcgaccagt gcgagagcaa   1860

cccctgcctg aacggcggca gctgcaagga cgacatcaac agctacgagt gctggtgccc   1920

cttcggcttc gagggcaaga actgcgagct ggacgtgacc tgcaacatca agaacggccg   1980

ctgcgagcag ttctgcaaga acagcgccga caacaaggtg gtgtgcagct gcaccgaggg   2040

ctaccgcctg gccgagaacc agaagagctg cgagcccgcc gtgcccttcc cctgcggccg   2100

cgtgagcgtg agccagacca gcaagctgac ccgcgccgag gccgtgttcc ccgacgtgga   2160

ctacgtgaac agcaccgagg ccgagaccat cctggacaac atcacccaga gcacccagag   2220

cttcaacgac ttcacccgcg tggtgggcgg cgaggacgcc aagcccggcc agttcccctg   2280

gcaggtggtg ctgaacggca aggtggacgc cttctgcggc ggcagcatcg tgaacgagaa   2340

gtggatcgtg accgccgccc actgcgtgga gaccggcgtg aagatcaccg tggtggccgg   2400

cgagcacaac atcgaggaga ccgagcacac cgagcagaag cgcaacgtga tccgcatcat   2460

cccccaccac aactacaacg ccgccatcaa caagtacaac cacgacatcg ccctgctgga   2520

gctggacgag cccctggtgc tgaacagcta cgtgaccccc atctgcatcg ccgacaagga   2580

gtacaccaac atcttcctga agttcggcag cggctacgtg agcggctggg gccgcgtgtt   2640

ccacaagggc cgcagcgccc tggtgctgca gtacctgcgc gtgcccctgg tggaccgcgc   2700

cacctgcctg cgcagcacca agttcaccat ctacaacaac atgttctgcg ccggcttcca   2760

cgagggcggc cgcgacagct gccagggcga cagcggcggc ccccacgtga ccgaggtgga   2820

gggcaccagc ttcctgaccg gcatcatcag ctggggcgag gagtgcgcca tgaagggcaa   2880

gtacggcatc tacaccaagg tgagccgcta cgtgaactgg atcaaggaga agaccaagct   2940

gacctggaga tctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc   3000

ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa   3060

aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg   3120

gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatctgata   3180

gcaggcatgc tggggagaga tcgatctagg aacccctagt gatggagttg gccactccct   3240

ctctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct   3300

ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aacccccccc   3360

cccccccccc tgcatgcagg cgattctctt gtttgctcca gactctcagg caatgacctg   3420

atagcctttg tagagacctc tcaaaaatag ctaccctctc cggcatgaat ttatcagcta   3480

gaacggttga atatcatatt gatggtgatt tgactgtctc cggcctttct cacccgtttg   3540

aatctttacc tacacattac tcaggcattg catttaaaat atatgagggt tctaaaaatt   3600

tttatccttg cgttgaaata aaggcttctc ccgcaaaagt attacagggt cataatgttt   3660

ttggtacaac cgatttagct ttatgctctg aggctttatt gcttaatttt gctaattctt   3720

tgccttgcct gtatgattta ttggatgttg gaattcctga tgcggtattt tctccttacg   3780

catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc   3840

gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt   3900

ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag   3960

aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt   4020

ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga   4080

aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc   4140

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt   4200

caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct   4260

cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt   4320

tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt   4380

tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac   4440

gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac   4500

tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct   4560

gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg   4620

aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg   4680

gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca   4740

atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa   4800

caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt   4860

ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc   4920

attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg   4980

agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt   5040

aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt   5100

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc   5160

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct   5220

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta   5280

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc   5340

ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac   5400

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct   5460

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat   5520

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg   5580

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa   5640

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg   5700

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga   5760

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc   5820

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct   5880

gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct   5940

cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca   6000

atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatg                    6044


<210>  2
<211>  6044
<212>  DNA
<213>  Artificial

<220>
<223>  Adenoviral expression vector pdsAAVsc SerpEnh TTRmin MVM 
       FIXcopt-PADUA-bghpA

<400>  2
cagcagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag     60

cctgaatggc gaatggaatt ccagacgatt gagcgtcaaa atgtaggtat ttccatgagc    120

gtttttcctg ttgcaatggc tggcggtaat attgttctgg atattaccag caaggccgat    180

agtttgagtt cttctactca ggcaagtgat gttattacta atcaaagaag tattgcgaca    240

acggttaatt tgcgtgatgg acagactctt ttactcggtg gcctcactga ttataaaaac    300

acttctcagg attctggcgt accgttcctg tctaaaatcc ctttaatcgg cctcctgttt    360

agctcccgct ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa agcaaccata    420

gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac    480

cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc    540

cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt    600

tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg    660

gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag    720

tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt    780

ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt    840

taacgcgaat tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt    900

cctgtttttg gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt    960

acgattaccg ttcatcgcct gcactgcgcg ctcgctcgct cactgaggcc gcccgggcaa   1020

agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag   1080

agggagtgga attcacgcgt ggtacgatct gaattcggta caattcacgc gtggtacggc   1140

cgcggtaccg gcgcgccggg ggaggctgct ggtgaatatt aaccaaggtc accccagtta   1200

tcggaggagc aaacaggggc taagtccaca cgcgtggtac cgtctgtctg cacatttcgt   1260

agagcgagtg ttccgatact ctaatctccc taggcaaggt tcatatttgt gtaggttact   1320

tattctcctt ttgttgacta agtcaataat cagaatcagc aggtttggag tcagcttggc   1380

agggatcagc agcctgggtt ggaaggaggg ggtataaaag ccccttcacc aggagaagcc   1440

gtcacacaga tccacaagct cctgaagagg taagggttta agggatggtt ggttggtggg   1500

gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggctag   1560

catgcagcgc gtgaacatga tcatggccga gagccccggc ctgatcacca tctgcctgct   1620

gggctacctg ctgagcgccg agtgcaccgt gttcctggac cacgagaacg ccaacaagat   1680

cctgaaccgc cccaagcgct acaacagcgg caagctggag gagttcgtgc agggcaacct   1740

ggagcgcgag tgcatggagg agaagtgcag cttcgaggag gcccgcgagg tgttcgagaa   1800

caccgagcgc accaccgagt tctggaagca gtacgtggac ggcgaccagt gcgagagcaa   1860

cccctgcctg aacggcggca gctgcaagga cgacatcaac agctacgagt gctggtgccc   1920

cttcggcttc gagggcaaga actgcgagct ggacgtgacc tgcaacatca agaacggccg   1980

ctgcgagcag ttctgcaaga acagcgccga caacaaggtg gtgtgcagct gcaccgaggg   2040

ctaccgcctg gccgagaacc agaagagctg cgagcccgcc gtgcccttcc cctgcggccg   2100

cgtgagcgtg agccagacca gcaagctgac ccgcgccgag gccgtgttcc ccgacgtgga   2160

ctacgtgaac agcaccgagg ccgagaccat cctggacaac atcacccaga gcacccagag   2220

cttcaacgac ttcacccgcg tggtgggcgg cgaggacgcc aagcccggcc agttcccctg   2280

gcaggtggtg ctgaacggca aggtggacgc cttctgcggc ggcagcatcg tgaacgagaa   2340

gtggatcgtg accgccgccc actgcgtgga gaccggcgtg aagatcaccg tggtggccgg   2400

cgagcacaac atcgaggaga ccgagcacac cgagcagaag cgcaacgtga tccgcatcat   2460

cccccaccac aactacaacg ccgccatcaa caagtacaac cacgacatcg ccctgctgga   2520

gctggacgag cccctggtgc tgaacagcta cgtgaccccc atctgcatcg ccgacaagga   2580

gtacaccaac atcttcctga agttcggcag cggctacgtg agcggctggg gccgcgtgtt   2640

ccacaagggc cgcagcgccc tggtgctgca gtacctgcgc gtgcccctgg tggaccgcgc   2700

cacctgcctg ctgagcacca agttcaccat ctacaacaac atgttctgcg ccggcttcca   2760

cgagggcggc cgcgacagct gccagggcga cagcggcggc ccccacgtga ccgaggtgga   2820

gggcaccagc ttcctgaccg gcatcatcag ctggggcgag gagtgcgcca tgaagggcaa   2880

gtacggcatc tacaccaagg tgagccgcta cgtgaactgg atcaaggaga agaccaagct   2940

gacctggaga tctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc   3000

ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa   3060

aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg   3120

gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatctgata   3180

gcaggcatgc tggggagaga tcgatctagg aacccctagt gatggagttg gccactccct   3240

ctctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct   3300

ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aacccccccc   3360

cccccccccc tgcatgcagg cgattctctt gtttgctcca gactctcagg caatgacctg   3420

atagcctttg tagagacctc tcaaaaatag ctaccctctc cggcatgaat ttatcagcta   3480

gaacggttga atatcatatt gatggtgatt tgactgtctc cggcctttct cacccgtttg   3540

aatctttacc tacacattac tcaggcattg catttaaaat atatgagggt tctaaaaatt   3600

tttatccttg cgttgaaata aaggcttctc ccgcaaaagt attacagggt cataatgttt   3660

ttggtacaac cgatttagct ttatgctctg aggctttatt gcttaatttt gctaattctt   3720

tgccttgcct gtatgattta ttggatgttg gaattcctga tgcggtattt tctccttacg   3780

catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc   3840

gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt   3900

ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag   3960

aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt   4020

ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga   4080

aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc   4140

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt   4200

caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct   4260

cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt   4320

tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt   4380

tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac   4440

gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac   4500

tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct   4560

gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg   4620

aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg   4680

gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca   4740

atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa   4800

caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt   4860

ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc   4920

attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg   4980

agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt   5040

aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt   5100

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc   5160

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct   5220

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta   5280

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc   5340

ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac   5400

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct   5460

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat   5520

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg   5580

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa   5640

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg   5700

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga   5760

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc   5820

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct   5880

gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct   5940

cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca   6000

atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatg                    6044


<210>  3
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  3
gccttctagt tgccagccat                                                 20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  probe

<400>  4
tgtttgcccc tcccccgtgc                                                 20


<210>  5
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  5
ggcaccttcc agggtcaag                                                  19


<210>  6
<211>  7849
<212>  DNA
<213>  Artificial

<220>
<223>  Adenoviral expression vector 
       AAVss-SerpEnh-TTRm-MVM-hFVIIIcopt-sv40pA


<220>
<221>  misc_feature
<222>  (508)..(508)
<223>  n is a, c, g, or t

<400>  6
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc     60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca    120

actccatcac taggggttcc tgcggccgcg gtaccggcgc gccgggggag gctgctggtg    180

aatattaacc aaggtcaccc cagttatcgg aggagcaaac aggggctaag tccacacgcg    240

tggtaccgtc tgtctgcaca tttcgtagag cgagtgttcc gatactctaa tctccctagg    300

caaggttcat atttgtgtag gttacttatt ctccttttgt tgactaagtc aataatcaga    360

atcagcaggt ttggagtcag cttggcaggg atcagcagcc tgggttggaa ggagggggta    420

taaaagcccc ttcaccagga gaagccgtca cacagatcca caagctcctg aagaggtaag    480

ggtttaaggg atggttggtt ggtggggnat taatgtttaa ttacctggag cacctgcctg    540

aaatcacttt ttttcaggtt ggctagtatg cagatcgagc tgtccacctg cttttttctg    600

tgcctgctgc ggttctgctt cagcgccacc cggcggtact acctgggcgc cgtggagctg    660

tcctgggact acatgcagag cgacctgggc gagctgcccg tggacgcccg gttccccccc    720

agagtgccca agagcttccc cttcaacacc agcgtggtgt acaagaaaac cctgttcgtg    780

gagttcaccg accacctgtt caatatcgcc aagcccaggc ccccctggat gggcctgctg    840

ggccccacca tccaggccga ggtgtacgac accgtggtga tcaccctgaa gaacatggcc    900

agccaccccg tgagcctgca cgccgtgggc gtgagctact ggaaggccag cgagggcgcc    960

gagtacgacg accagaccag ccagcgggag aaagaagatg acaaggtgtt ccctggcggc   1020

agccacacct acgtgtggca ggtgctgaaa gaaaacggcc ccatggcctc cgaccccctg   1080

tgcctgacct acagctacct gagccacgtg gacctggtga aggacctgaa cagcggcctg   1140

atcggcgctc tgctcgtctg ccgggagggc agcctggcca aagagaaaac ccagaccctg   1200

cacaagttca tcctgctgtt cgccgtgttc gacgagggca agagctggca cagcgagaca   1260

aagaacagcc tgatgcagga ccgggacgcc gcctctgcca gagcctggcc caagatgcac   1320

accgtgaacg gctacgtgaa cagaagcctg cccggcctga ttggctgcca ccggaagagc   1380

gtgtactggc acgtgatcgg catgggcacc acacccgagg tgcacagcat ctttctggaa   1440

gggcacacct ttctggtccg gaaccaccgg caggccagcc tggaaatcag ccctatcacc   1500

ttcctgaccg cccagacact gctgatggac ctgggccagt tcctgctgtt ttgccacatc   1560

agctctcacc agcacgacgg catggaagcc tacgtgaagg tggactcttg ccccgaggaa   1620

ccccagctgc ggatgaagaa caacgaggaa gccgaggact acgacgacga cctgaccgac   1680

agcgagatgg acgtggtgcg gttcgacgac gacaacagcc ccagcttcat ccagatcaga   1740

agcgtggcca agaagcaccc caagacctgg gtgcactata tcgccgccga ggaagaggac   1800

tgggactacg cccccctggt gctggccccc gacgacagaa gctacaagag ccagtacctg   1860

aacaatggcc cccagcggat cggccggaag tacaagaaag tgcggttcat ggcctacacc   1920

gacgagacat tcaagacccg ggaggccatc cagcacgaga gcggcatcct gggccccctg   1980

ctgtacggcg aagtgggcga cacactgctg atcatcttca agaaccaggc tagccggccc   2040

tacaacatct acccccacgg catcaccgac gtgcggcccc tgtacagcag gcggctgccc   2100

aagggcgtga agcacctgaa ggacttcccc atcctgcccg gcgagatctt caagtacaag   2160

tggaccgtga ccgtggagga cggccccacc aagagcgacc ccagatgcct gacccggtac   2220

tacagcagct tcgtgaacat ggaacgggac ctggcctccg ggctgatcgg acctctgctg   2280

atctgctaca aagaaagcgt ggaccagcgg ggcaaccaga tcatgagcga caagcggaac   2340

gtgatcctgt tcagcgtgtt cgatgagaac cggtcctggt atctgaccga gaacatccag   2400

cggtttctgc ccaaccctgc cggcgtgcag ctggaagatc ccgagttcca ggccagcaac   2460

atcatgcact ccatcaatgg ctacgtgttc gactctctgc agctctccgt gtgtctgcac   2520

gaggtggcct actggtacat cctgagcatc ggcgcccaga ccgacttcct gagcgtgttc   2580

ttcagcggct acaccttcaa gcacaagatg gtgtacgagg acaccctgac cctgttccct   2640

ttcagcggcg agacagtgtt catgagcatg gaaaaccccg gcctgtggat tctgggctgc   2700

cacaacagcg acttccggaa ccggggcatg accgccctgc tgaaggtgtc cagctgcgac   2760

aagaacaccg gcgactacta cgaggacagc tacgaggata tcagcgccta cctgctgtcc   2820

aagaacaacg ccatcgaacc ccggagcttc agccagaacc cccccgtgct gacgcgtcac   2880

cagcgggaga tcacccggac aaccctgcag tccgaccagg aagagatcga ttacgacgac   2940

accatcagcg tggagatgaa gaaagaggat ttcgatatct acgacgagga cgagaaccag   3000

agccccagaa gcttccagaa gaaaacccgg cactacttca ttgccgccgt ggagaggctg   3060

tgggactacg gcatgagttc tagcccccac gtgctgcgga accgggccca gagcggcagc   3120

gtgccccagt tcaagaaagt ggtgttccag gaattcacag acggcagctt cacccagcct   3180

ctgtatagag gcgagctgaa cgagcacctg gggctgctgg ggccctacat cagggccgaa   3240

gtggaggaca acatcatggt gaccttccgg aatcaggcca gcagacccta ctccttctac   3300

agcagcctga tcagctacga agaggaccag cggcagggcg ccgaaccccg gaagaacttc   3360

gtgaagccca acgaaaccaa gacctacttc tggaaagtgc agcaccacat ggcccccacc   3420

aaggacgagt tcgactgcaa ggcctgggcc tacttcagcg acgtggatct ggaaaaggac   3480

gtgcactctg gactgattgg cccactcctg gtctgccaca ctaacaccct caaccccgcc   3540

cacggccgcc aggtgaccgt gcaggaattc gccctgttct tcaccatctt cgacgagaca   3600

aagtcctggt acttcaccga gaatatggaa cggaactgca gagccccctg caacatccag   3660

atggaagatc ctaccttcaa agagaactac cggttccacg ccatcaacgg ctacatcatg   3720

gacaccctgc ctggcctggt gatggcccag gaccagagaa tccggtggta tctgctgtcc   3780

atgggcagca acgagaatat ccacagcatc cacttcagcg gccacgtgtt caccgtgcgg   3840

aagaaagaag agtacaagat ggccctgtac aacctgtacc ccggcgtgtt cgagacagtg   3900

gagatgctgc ccagcaaggc cggcatctgg cgggtggagt gtctgatcgg cgagcacctg   3960

cacgctggca tgagcaccct gtttctggtg tacagcaaca agtgccagac cccactgggc   4020

atggcctctg gccacatccg ggacttccag atcaccgcct ccggccagta cggccagtgg   4080

gcccccaagc tggccagact gcactacagc ggcagcatca acgcctggtc caccaaagag   4140

cccttcagct ggatcaaggt ggacctgctg gcccctatga tcatccacgg cattaagacc   4200

cagggcgcca ggcagaagtt cagcagcctg tacatcagcc agttcatcat catgtacagc   4260

ctggacggca agaagtggca gacctaccgg ggcaacagca ccggcaccct gatggtgttc   4320

ttcggcaatg tggacagcag cggcatcaag cacaacatct tcaacccccc catcattgcc   4380

cggtacatcc ggctgcaccc cacccactac agcattagat ccacactgag aatggaactg   4440

atgggctgcg acctgaactc ctgcagcatg cctctgggca tggaaagcaa ggccatcagc   4500

gacgcccaga tcacagccag cagctacttc accaacatgt tcgccacctg gtccccctcc   4560

aaggccaggc tgcacctgca gggccggtcc aacgcctggc ggcctcaggt caacaacccc   4620

aaagaatggc tgcaggtgga ctttcagaaa accatgaagg tgaccggcgt gaccacccag   4680

ggcgtgaaaa gcctgctgac cagcatgtac gtgaaagagt ttctgatcag cagctctcag   4740

gatggccacc agtggaccct gttctttcag aacggcaagg tgaaagtgtt ccagggcaac   4800

caggactcct tcacccccgt ggtgaactcc ctggaccccc ccctgctgac ccgctacctg   4860

agaatccacc cccagtcttg ggtgcaccag atcgccctca ggatggaagt cctgggatgt   4920

gaggcccagg atctgtactg atgaggatct aggctcgaca tgctttattt gtgaaatttg   4980

tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa   5040

ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaactcgaga   5100

tccacggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc   5160

gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc   5220

agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac   5280

gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc   5340

ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc   5400

gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt   5460

ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac   5520

ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag   5580

acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa   5640

actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg   5700

atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac   5760

aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca   5820

tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg   5880

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg   5940

ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta   6000

taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat   6060

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg   6120

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa   6180

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac   6240

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac   6300

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt   6360

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc   6420

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca   6480

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc   6540

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag   6600

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa   6660

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg   6720

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa   6780

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg   6840

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt   6900

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt   6960

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag   7020

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat   7080

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct   7140

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct   7200

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca   7260

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc   7320

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc   7380

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct   7440

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag   7500

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc   7560

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg   7620

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag   7680

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt   7740

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac   7800

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt               7849


<210>  7
<211>  4377
<212>  DNA
<213>  Artificial

<220>
<223>  Codon-optimized B domain deleted human coagulation factor VIII 
       (hFVIIIcopt)

<400>  7
atgcagatcg agctgtccac ctgctttttt ctgtgcctgc tgcggttctg cttcagcgcc     60

acccggcggt actacctggg cgccgtggag ctgtcctggg actacatgca gagcgacctg    120

ggcgagctgc ccgtggacgc ccggttcccc cccagagtgc ccaagagctt ccccttcaac    180

accagcgtgg tgtacaagaa aaccctgttc gtggagttca ccgaccacct gttcaatatc    240

gccaagccca ggcccccctg gatgggcctg ctgggcccca ccatccaggc cgaggtgtac    300

gacaccgtgg tgatcaccct gaagaacatg gccagccacc ccgtgagcct gcacgccgtg    360

ggcgtgagct actggaaggc cagcgagggc gccgagtacg acgaccagac cagccagcgg    420

gagaaagaag atgacaaggt gttccctggc ggcagccaca cctacgtgtg gcaggtgctg    480

aaagaaaacg gccccatggc ctccgacccc ctgtgcctga cctacagcta cctgagccac    540

gtggacctgg tgaaggacct gaacagcggc ctgatcggcg ctctgctcgt ctgccgggag    600

ggcagcctgg ccaaagagaa aacccagacc ctgcacaagt tcatcctgct gttcgccgtg    660

ttcgacgagg gcaagagctg gcacagcgag acaaagaaca gcctgatgca ggaccgggac    720

gccgcctctg ccagagcctg gcccaagatg cacaccgtga acggctacgt gaacagaagc    780

ctgcccggcc tgattggctg ccaccggaag agcgtgtact ggcacgtgat cggcatgggc    840

accacacccg aggtgcacag catctttctg gaagggcaca cctttctggt ccggaaccac    900

cggcaggcca gcctggaaat cagccctatc accttcctga ccgcccagac actgctgatg    960

gacctgggcc agttcctgct gttttgccac atcagctctc accagcacga cggcatggaa   1020

gcctacgtga aggtggactc ttgccccgag gaaccccagc tgcggatgaa gaacaacgag   1080

gaagccgagg actacgacga cgacctgacc gacagcgaga tggacgtggt gcggttcgac   1140

gacgacaaca gccccagctt catccagatc agaagcgtgg ccaagaagca ccccaagacc   1200

tgggtgcact atatcgccgc cgaggaagag gactgggact acgcccccct ggtgctggcc   1260

cccgacgaca gaagctacaa gagccagtac ctgaacaatg gcccccagcg gatcggccgg   1320

aagtacaaga aagtgcggtt catggcctac accgacgaga cattcaagac ccgggaggcc   1380

atccagcacg agagcggcat cctgggcccc ctgctgtacg gcgaagtggg cgacacactg   1440

ctgatcatct tcaagaacca ggctagccgg ccctacaaca tctaccccca cggcatcacc   1500

gacgtgcggc ccctgtacag caggcggctg cccaagggcg tgaagcacct gaaggacttc   1560

cccatcctgc ccggcgagat cttcaagtac aagtggaccg tgaccgtgga ggacggcccc   1620

accaagagcg accccagatg cctgacccgg tactacagca gcttcgtgaa catggaacgg   1680

gacctggcct ccgggctgat cggacctctg ctgatctgct acaaagaaag cgtggaccag   1740

cggggcaacc agatcatgag cgacaagcgg aacgtgatcc tgttcagcgt gttcgatgag   1800

aaccggtcct ggtatctgac cgagaacatc cagcggtttc tgcccaaccc tgccggcgtg   1860

cagctggaag atcccgagtt ccaggccagc aacatcatgc actccatcaa tggctacgtg   1920

ttcgactctc tgcagctctc cgtgtgtctg cacgaggtgg cctactggta catcctgagc   1980

atcggcgccc agaccgactt cctgagcgtg ttcttcagcg gctacacctt caagcacaag   2040

atggtgtacg aggacaccct gaccctgttc cctttcagcg gcgagacagt gttcatgagc   2100

atggaaaacc ccggcctgtg gattctgggc tgccacaaca gcgacttccg gaaccggggc   2160

atgaccgccc tgctgaaggt gtccagctgc gacaagaaca ccggcgacta ctacgaggac   2220

agctacgagg atatcagcgc ctacctgctg tccaagaaca acgccatcga accccggagc   2280

ttcagccaga acccccccgt gctgacgcgt caccagcggg agatcacccg gacaaccctg   2340

cagtccgacc aggaagagat cgattacgac gacaccatca gcgtggagat gaagaaagag   2400

gatttcgata tctacgacga ggacgagaac cagagcccca gaagcttcca gaagaaaacc   2460

cggcactact tcattgccgc cgtggagagg ctgtgggact acggcatgag ttctagcccc   2520

cacgtgctgc ggaaccgggc ccagagcggc agcgtgcccc agttcaagaa agtggtgttc   2580

caggaattca cagacggcag cttcacccag cctctgtata gaggcgagct gaacgagcac   2640

ctggggctgc tggggcccta catcagggcc gaagtggagg acaacatcat ggtgaccttc   2700

cggaatcagg ccagcagacc ctactccttc tacagcagcc tgatcagcta cgaagaggac   2760

cagcggcagg gcgccgaacc ccggaagaac ttcgtgaagc ccaacgaaac caagacctac   2820

ttctggaaag tgcagcacca catggccccc accaaggacg agttcgactg caaggcctgg   2880

gcctacttca gcgacgtgga tctggaaaag gacgtgcact ctggactgat tggcccactc   2940

ctggtctgcc acactaacac cctcaacccc gcccacggcc gccaggtgac cgtgcaggaa   3000

ttcgccctgt tcttcaccat cttcgacgag acaaagtcct ggtacttcac cgagaatatg   3060

gaacggaact gcagagcccc ctgcaacatc cagatggaag atcctacctt caaagagaac   3120

taccggttcc acgccatcaa cggctacatc atggacaccc tgcctggcct ggtgatggcc   3180

caggaccaga gaatccggtg gtatctgctg tccatgggca gcaacgagaa tatccacagc   3240

atccacttca gcggccacgt gttcaccgtg cggaagaaag aagagtacaa gatggccctg   3300

tacaacctgt accccggcgt gttcgagaca gtggagatgc tgcccagcaa ggccggcatc   3360

tggcgggtgg agtgtctgat cggcgagcac ctgcacgctg gcatgagcac cctgtttctg   3420

gtgtacagca acaagtgcca gaccccactg ggcatggcct ctggccacat ccgggacttc   3480

cagatcaccg cctccggcca gtacggccag tgggccccca agctggccag actgcactac   3540

agcggcagca tcaacgcctg gtccaccaaa gagcccttca gctggatcaa ggtggacctg   3600

ctggccccta tgatcatcca cggcattaag acccagggcg ccaggcagaa gttcagcagc   3660

ctgtacatca gccagttcat catcatgtac agcctggacg gcaagaagtg gcagacctac   3720

cggggcaaca gcaccggcac cctgatggtg ttcttcggca atgtggacag cagcggcatc   3780

aagcacaaca tcttcaaccc ccccatcatt gcccggtaca tccggctgca ccccacccac   3840

tacagcatta gatccacact gagaatggaa ctgatgggct gcgacctgaa ctcctgcagc   3900

atgcctctgg gcatggaaag caaggccatc agcgacgccc agatcacagc cagcagctac   3960

ttcaccaaca tgttcgccac ctggtccccc tccaaggcca ggctgcacct gcagggccgg   4020

tccaacgcct ggcggcctca ggtcaacaac cccaaagaat ggctgcaggt ggactttcag   4080

aaaaccatga aggtgaccgg cgtgaccacc cagggcgtga aaagcctgct gaccagcatg   4140

tacgtgaaag agtttctgat cagcagctct caggatggcc accagtggac cctgttcttt   4200

cagaacggca aggtgaaagt gttccagggc aaccaggact ccttcacccc cgtggtgaac   4260

tccctggacc cccccctgct gacccgctac ctgagaatcc acccccagtc ttgggtgcac   4320

cagatcgccc tcaggatgga agtcctggga tgtgaggccc aggatctgta ctgatga      4377


<210>  8
<211>  72
<212>  DNA
<213>  Artificial

<220>
<223>  Serpin enhancer (SerpEnh)

<400>  8
gggggaggct gctggtgaat attaaccaag gtcaccccag ttatcggagg agcaaacagg     60

ggctaagtcc ac                                                         72


<210>  9
<211>  202
<212>  DNA
<213>  Artificial

<220>
<223>  Minimal transthyretin promoter (TTRm)

<400>  9
gtctgtctgc acatttcgta gagcgagtgt tccgatactc taatctccct aggcaaggtt     60

catatttgtg taggttactt attctccttt tgttgactaa gtcaataatc agaatcagca    120

ggtttggagt cagcttggca gggatcagca gcctgggttg gaaggagggg gtataaaagc    180

cccttcacca ggagaagccg tc                                             202


<210>  10
<211>  92
<212>  DNA
<213>  Artificial

<220>
<223>  Minute virus mouse (MVM) intron


<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  n is a, c, g, or t

<400>  10
aagaggtaag ggtttaaggg atggttggtt ggtggggnat taatgtttaa ttacctggag     60

cacctgcctg aaatcacttt ttttcaggtt gg                                   92


<210>  11
<211>  134
<212>  DNA
<213>  Artificial

<220>
<223>  Simian virus 40 (SV40) polyadenylation signal (pA)

<400>  11
atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa     60

taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg    120

ggaggttttt taaa                                                      134


<210>  12
<211>  7653
<212>  DNA
<213>  Artificial

<220>
<223>  pcDNA3_mouseCO_hyPiggyBac_Transposase_MT plasmid

<400>  12
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg     60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg    120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc    180

ttagggttag gcgttttgcg ctgcttcgcg cgtggagcta gttattaata gtaatcaatt    240

acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat    300

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt    360

cccatagtaa cgtcaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa    420

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc    480

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct    540

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag    600

tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt    660

gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac    720

aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc    780

agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc    840

catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc gggaacggtg    900

cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag    960

gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact   1020

ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca   1080

ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa   1140

atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac   1200

aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc   1260

caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg   1320

gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga ttcgaacatc   1380

gatgccgcca ccatgggcag cagcctggac gacgagcaca tcctgagcgc cctgctgcag   1440

agcgacgacg agctggtcgg cgaggacagc gacagcgagg tgagcgacca cgtgagcgag   1500

gacgacgtgc agtccgacac cgaggaggcc ttcatcgacg aggtgcacga ggtgcagcct   1560

accagcagcg gctccgagat cctggacgag cagaacgtga tcgagcagcc cggcagctcc   1620

ctggccagca acaggatcct gaccctgccc cagaggacca tcaggggcaa gaacaagcac   1680

tgctggtcca cctccaagcc caccaggcgg agcagggtgt ccgccctgaa catcgtgaga   1740

agccagaggg gccccaccag gatgtgcagg aacatctacg accccctgct gtgcttcaag   1800

ctgttcttca ccgacgagat catcagcgag atcgtgaagt ggaccaacgc cgagatcagc   1860

ctgaagaggc gggagagcat gaccagcgcc accttcaggg acaccaacga ggacgagatc   1920

tacgccttct tcggcatcct ggtgatgacc gccgtgagga aggacaacca catgagcacc   1980

gacgacctgt tcgacagatc cctgagcatg gtgtacgtga gcgtgatgag cagggacaga   2040

ttcgacttcc tgatcagatg cctgaggatg gacgacaaga gcatcaggcc caccctgcgg   2100

gagaacgacg tgttcacccc cgtgagaaag atctgggacc tgttcatcca ccagtgcatc   2160

cagaactaca cccctggcgc ccacctgacc atcgacgagc agctgctggg cttcaggggc   2220

aggtgcccct tcagggtgta tatccccaac aagcccagca agtacggcat caagatcctg   2280

atgatgtgcg acagcggcac caagtacatg atcaacggca tgccctacct gggcaggggc   2340

acccagacca acggcgtgcc cctgggcgag tactacgtga aggagctgtc caagcccgtc   2400

cacggcagct gcagaaacat cacctgcgac aactggttca ccagcatccc cctggccaag   2460

aacctgctgc aggagcccta caagctgacc atcgtgggca ccgtgagaag caacaagaga   2520

gagatccccg aggtcctgaa gaacagcagg tccaggcccg tgggcaccag catgttctgc   2580

ttcgacggcc ccctgaccct ggtgtcctac aagcccaagc ccgccaagat ggtgtacctg   2640

ctgtccagct gcgacgagga cgccagcatc aacgagagca ccggcaagcc ccagatggtg   2700

atgtactaca accagaccaa gggcggcgtg gacaccctgg accagatgtg cagcgtgatg   2760

acctgcagca gaaagaccaa caggtggccc atggccctgc tgtacggcat gatcaacatc   2820

gcctgcatca acagcttcat catctacagc cacaacgtga gcagcaaggg cgagaaggtg   2880

cagagccgga aaaagttcat gcggaacctg tacatgggcc tgacctccag cttcatgagg   2940

aagaggctgg aggcccccac cctgaagaga tacctgaggg acaacatcag caacatcctg   3000

cccaaggagg tgcccggcac cagcgacgac agcaccgagg agcccgtgat gaagaagagg   3060

acctactgca cctactgtcc cagcaagatc agaagaaagg ccagcgccag ctgcaagaag   3120

tgtaagaagg tcatctgccg ggagcacaac atcgacatgt gccagagctg tttctgactc   3180

gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc   3240

agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc   3300

cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc   3360

gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg   3420

ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga   3480

ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt   3540

aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc   3600

gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca   3660

agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc   3720

caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt   3780

tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac   3840

aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc   3900

ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat   3960

gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag   4020

catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag   4080

aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc   4140

catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt   4200

ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg   4260

aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt   4320

cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca   4380

cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac   4440

aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt   4500

tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc   4560

gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg   4620

aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc   4680

tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc   4740

ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat   4800

ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc   4860

cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca   4920

tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga   4980

ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat   5040

tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc   5100

tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact   5160

ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc   5220

accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg   5280

atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca   5340

gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt   5400

tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata   5460

ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat   5520

tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg   5580

ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag   5640

tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt   5700

ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg   5760

ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg   5820

gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag   5880

gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga   5940

cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct   6000

ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc   6060

tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg   6120

gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc   6180

tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca   6240

ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag   6300

ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct   6360

ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc   6420

accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga   6480

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca   6540

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat   6600

taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac   6660

caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt   6720

gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt   6780

gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag   6840

ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct   6900

attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt   6960

gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc   7020

tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt   7080

agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg   7140

gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg   7200

actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct   7260

tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc   7320

attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt   7380

tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt   7440

tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg   7500

aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat   7560

tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg   7620

cgcacatttc cccgaaaagt gccacctgac gtc                                7653


<210>  13
<211>  8514
<212>  DNA
<213>  Artificial

<220>
<223>  PB_Minimal_T_(T53C-C136T)_D4Z4_TTRminSerpMVM_hFVIIIcopt_SV40pA_D4
       Z4 transposon


<220>
<221>  misc_feature
<222>  (1335)..(1335)
<223>  n is a, c, g, or t

<400>  13
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc     60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga    120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc    180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc    240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag    300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa    360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac    420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg    480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg    540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg    600

taaaacgacg gccagtgagc gcgcttaacc ctagaaagat aatcatattg tgacgtacgt    660

taaagataat catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta    720

tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac taataataaa    780

ttcaacaaac aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt    840

tcttctataa agtaacaaaa cttttatcga taacttcgta taatgtatgc tatacgaagt    900

tatagagggg cggaagggac gttaggaggg aggcagggag gcagggaggc agggaggaac    960

ggagggaggc ggccgcggta ccggcgcgcc gggggaggct gctggtgaat attaaccaag   1020

gtcaccccag ttatcggagg agcaaacagg ggctaagtcc acacgcgtgg taccgtctgt   1080

ctgcacattt cgtagagcga gtgttccgat actctaatct ccctaggcaa ggttcatatt   1140

tgtgtaggtt acttattctc cttttgttga ctaagtcaat aatcagaatc agcaggtttg   1200

gagtcagctt ggcagggatc agcagcctgg gttggaagga gggggtataa aagccccttc   1260

accaggagaa gccgtcacac agatccacaa gctcctgaag aggtaagggt ttaagggatg   1320

gttggttggt ggggnattaa tgtttaatta cctggagcac ctgcctgaaa tcactttttt   1380

tcaggttggc tagtatgcag atcgagctgt ccacctgctt ttttctgtgc ctgctgcggt   1440

tctgcttcag cgccacccgg cggtactacc tgggcgccgt ggagctgtcc tgggactaca   1500

tgcagagcga cctgggcgag ctgcccgtgg acgcccggtt cccccccaga gtgcccaaga   1560

gcttcccctt caacaccagc gtggtgtaca agaaaaccct gttcgtggag ttcaccgacc   1620

acctgttcaa tatcgccaag cccaggcccc cctggatggg cctgctgggc cccaccatcc   1680

aggccgaggt gtacgacacc gtggtgatca ccctgaagaa catggccagc caccccgtga   1740

gcctgcacgc cgtgggcgtg agctactgga aggccagcga gggcgccgag tacgacgacc   1800

agaccagcca gcgggagaaa gaagatgaca aggtgttccc tggcggcagc cacacctacg   1860

tgtggcaggt gctgaaagaa aacggcccca tggcctccga ccccctgtgc ctgacctaca   1920

gctacctgag ccacgtggac ctggtgaagg acctgaacag cggcctgatc ggcgctctgc   1980

tcgtctgccg ggagggcagc ctggccaaag agaaaaccca gaccctgcac aagttcatcc   2040

tgctgttcgc cgtgttcgac gagggcaaga gctggcacag cgagacaaag aacagcctga   2100

tgcaggaccg ggacgccgcc tctgccagag cctggcccaa gatgcacacc gtgaacggct   2160

acgtgaacag aagcctgccc ggcctgattg gctgccaccg gaagagcgtg tactggcacg   2220

tgatcggcat gggcaccaca cccgaggtgc acagcatctt tctggaaggg cacacctttc   2280

tggtccggaa ccaccggcag gccagcctgg aaatcagccc tatcaccttc ctgaccgccc   2340

agacactgct gatggacctg ggccagttcc tgctgttttg ccacatcagc tctcaccagc   2400

acgacggcat ggaagcctac gtgaaggtgg actcttgccc cgaggaaccc cagctgcgga   2460

tgaagaacaa cgaggaagcc gaggactacg acgacgacct gaccgacagc gagatggacg   2520

tggtgcggtt cgacgacgac aacagcccca gcttcatcca gatcagaagc gtggccaaga   2580

agcaccccaa gacctgggtg cactatatcg ccgccgagga agaggactgg gactacgccc   2640

ccctggtgct ggcccccgac gacagaagct acaagagcca gtacctgaac aatggccccc   2700

agcggatcgg ccggaagtac aagaaagtgc ggttcatggc ctacaccgac gagacattca   2760

agacccggga ggccatccag cacgagagcg gcatcctggg ccccctgctg tacggcgaag   2820

tgggcgacac actgctgatc atcttcaaga accaggctag ccggccctac aacatctacc   2880

cccacggcat caccgacgtg cggcccctgt acagcaggcg gctgcccaag ggcgtgaagc   2940

acctgaagga cttccccatc ctgcccggcg agatcttcaa gtacaagtgg accgtgaccg   3000

tggaggacgg ccccaccaag agcgacccca gatgcctgac ccggtactac agcagcttcg   3060

tgaacatgga acgggacctg gcctccgggc tgatcggacc tctgctgatc tgctacaaag   3120

aaagcgtgga ccagcggggc aaccagatca tgagcgacaa gcggaacgtg atcctgttca   3180

gcgtgttcga tgagaaccgg tcctggtatc tgaccgagaa catccagcgg tttctgccca   3240

accctgccgg cgtgcagctg gaagatcccg agttccaggc cagcaacatc atgcactcca   3300

tcaatggcta cgtgttcgac tctctgcagc tctccgtgtg tctgcacgag gtggcctact   3360

ggtacatcct gagcatcggc gcccagaccg acttcctgag cgtgttcttc agcggctaca   3420

ccttcaagca caagatggtg tacgaggaca ccctgaccct gttccctttc agcggcgaga   3480

cagtgttcat gagcatggaa aaccccggcc tgtggattct gggctgccac aacagcgact   3540

tccggaaccg gggcatgacc gccctgctga aggtgtccag ctgcgacaag aacaccggcg   3600

actactacga ggacagctac gaggatatca gcgcctacct gctgtccaag aacaacgcca   3660

tcgaaccccg gagcttcagc cagaaccccc ccgtgctgac gcgtcaccag cgggagatca   3720

cccggacaac cctgcagtcc gaccaggaag agatcgatta cgacgacacc atcagcgtgg   3780

agatgaagaa agaggatttc gatatctacg acgaggacga gaaccagagc cccagaagct   3840

tccagaagaa aacccggcac tacttcattg ccgccgtgga gaggctgtgg gactacggca   3900

tgagttctag cccccacgtg ctgcggaacc gggcccagag cggcagcgtg ccccagttca   3960

agaaagtggt gttccaggaa ttcacagacg gcagcttcac ccagcctctg tatagaggcg   4020

agctgaacga gcacctgggg ctgctggggc cctacatcag ggccgaagtg gaggacaaca   4080

tcatggtgac cttccggaat caggccagca gaccctactc cttctacagc agcctgatca   4140

gctacgaaga ggaccagcgg cagggcgccg aaccccggaa gaacttcgtg aagcccaacg   4200

aaaccaagac ctacttctgg aaagtgcagc accacatggc ccccaccaag gacgagttcg   4260

actgcaaggc ctgggcctac ttcagcgacg tggatctgga aaaggacgtg cactctggac   4320

tgattggccc actcctggtc tgccacacta acaccctcaa ccccgcccac ggccgccagg   4380

tgaccgtgca ggaattcgcc ctgttcttca ccatcttcga cgagacaaag tcctggtact   4440

tcaccgagaa tatggaacgg aactgcagag ccccctgcaa catccagatg gaagatccta   4500

ccttcaaaga gaactaccgg ttccacgcca tcaacggcta catcatggac accctgcctg   4560

gcctggtgat ggcccaggac cagagaatcc ggtggtatct gctgtccatg ggcagcaacg   4620

agaatatcca cagcatccac ttcagcggcc acgtgttcac cgtgcggaag aaagaagagt   4680

acaagatggc cctgtacaac ctgtaccccg gcgtgttcga gacagtggag atgctgccca   4740

gcaaggccgg catctggcgg gtggagtgtc tgatcggcga gcacctgcac gctggcatga   4800

gcaccctgtt tctggtgtac agcaacaagt gccagacccc actgggcatg gcctctggcc   4860

acatccggga cttccagatc accgcctccg gccagtacgg ccagtgggcc cccaagctgg   4920

ccagactgca ctacagcggc agcatcaacg cctggtccac caaagagccc ttcagctgga   4980

tcaaggtgga cctgctggcc cctatgatca tccacggcat taagacccag ggcgccaggc   5040

agaagttcag cagcctgtac atcagccagt tcatcatcat gtacagcctg gacggcaaga   5100

agtggcagac ctaccggggc aacagcaccg gcaccctgat ggtgttcttc ggcaatgtgg   5160

acagcagcgg catcaagcac aacatcttca acccccccat cattgcccgg tacatccggc   5220

tgcaccccac ccactacagc attagatcca cactgagaat ggaactgatg ggctgcgacc   5280

tgaactcctg cagcatgcct ctgggcatgg aaagcaaggc catcagcgac gcccagatca   5340

cagccagcag ctacttcacc aacatgttcg ccacctggtc cccctccaag gccaggctgc   5400

acctgcaggg ccggtccaac gcctggcggc ctcaggtcaa caaccccaaa gaatggctgc   5460

aggtggactt tcagaaaacc atgaaggtga ccggcgtgac cacccagggc gtgaaaagcc   5520

tgctgaccag catgtacgtg aaagagtttc tgatcagcag ctctcaggat ggccaccagt   5580

ggaccctgtt ctttcagaac ggcaaggtga aagtgttcca gggcaaccag gactccttca   5640

cccccgtggt gaactccctg gacccccccc tgctgacccg ctacctgaga atccaccccc   5700

agtcttgggt gcaccagatc gccctcagga tggaagtcct gggatgtgag gcccaggatc   5760

tgtactgatg aggatctagg ctcgacatgc tttatttgtg aaatttgtga tgctattgct   5820

ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt   5880

atgtttcagg ttcaggggga ggtgtgggag gttttttaaa ctcgagaccg gtagaggggc   5940

ggaagggacg ttaggaggga ggcagggagg cagggaggca gggaggaacg gagggagata   6000

acttcgtata atgtatgcta tacgaagtta tgatatctat aacaagaaaa tatatatata   6060

ataagttatc acgtaagtag aacacgaaat aacaatataa ttatcgtatg agttaaatct   6120

taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggttgt tatagttcaa   6180

aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga   6240

gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa   6300

gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaagcgcgc ttggcgtaat   6360

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac   6420

gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa   6480

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat   6540

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc   6600

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg   6660

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag   6720

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc   6780

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag   6840

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga   6900

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc   6960

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg   7020

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt   7080

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca   7140

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca   7200

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag   7260

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca   7320

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg   7380

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa   7440

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta   7500

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag   7560

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga   7620

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac   7680

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc   7740

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta   7800

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac   7860

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat   7920

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa   7980

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg   8040

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag   8100

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc   8160

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct   8220

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat   8280

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg   8340

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc   8400

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta   8460

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac         8514


<210>  14
<211>  5097
<212>  DNA
<213>  Artificial

<220>
<223>  PB_micro_T_No_ins_TTRminSerpMVM_FIXco_bghpA transposon

<400>  14
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc     60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga    120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc    180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc    240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag    300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa    360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac    420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg    480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg    540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg    600

taaaacgacg gccagtgagc gcgcttaacc ctagaaagat aatcatattg tgacgtacgt    660

taaagataat catgcgtaaa attgacgcat gataacttcg tataatgtat gctatacgaa    720

gttatgcggc cgcggtaccg gcgcgccggg ggaggctgct ggtgaatatt aaccaaggtc    780

accccagtta tcggaggagc aaacaggggc taagtccaca cgcgtggtac cgtctgtctg    840

cacatttcgt agagcgagtg ttccgatact ctaatctccc taggcaaggt tcatatttgt    900

gtaggttact tattctcctt ttgttgacta agtcaataat cagaatcagc aggtttggag    960

tcagcttggc agggatcagc agcctgggtt ggaaggaggg ggtataaaag ccccttcacc   1020

aggagaagcc gtcacacaga tccacaagct cctgaagagg taagggttta agggatggtt   1080

ggttggtggg gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca   1140

ggttgggcta gcccaccatg cagcgcgtga acatgatcat ggccgagagc cccggcctga   1200

tcaccatctg cctgctgggc tacctgctga gcgccgagtg caccgtgttc ctggaccacg   1260

agaacgccaa caagatcctg aaccgcccca agcgctacaa cagcggcaag ctggaggagt   1320

tcgtgcaggg caacctggag cgcgagtgca tggaggagaa gtgcagcttc gaggaggccc   1380

gcgaggtgtt cgagaacacc gagcgcacca ccgagttctg gaagcagtac gtggacggcg   1440

accagtgcga gagcaacccc tgcctgaacg gcggcagctg caaggacgac atcaacagct   1500

acgagtgctg gtgccccttc ggcttcgagg gcaagaactg cgagctggac gtgacctgca   1560

acatcaagaa cggccgctgc gagcagttct gcaagaacag cgccgacaac aaggtggtgt   1620

gcagctgcac cgagggctac cgcctggccg agaaccagaa gagctgcgag cccgccgtgc   1680

ccttcccctg cggccgcgtg agcgtgagcc agaccagcaa gctgacccgc gccgaggccg   1740

tgttccccga cgtggactac gtgaacagca ccgaggccga gaccatcctg gacaacatca   1800

cccagagcac ccagagcttc aacgacttca cccgcgtggt gggcggcgag gacgccaagc   1860

ccggccagtt cccctggcag gtggtgctga acggcaaggt ggacgccttc tgcggcggca   1920

gcatcgtgaa cgagaagtgg atcgtgaccg ccgcccactg cgtggagacc ggcgtgaaga   1980

tcaccgtggt ggccggcgag cacaacatcg aggagaccga gcacaccgag cagaagcgca   2040

acgtgatccg catcatcccc caccacaact acaacgccgc catcaacaag tacaaccacg   2100

acatcgccct gctggagctg gacgagcccc tggtgctgaa cagctacgtg acccccatct   2160

gcatcgccga caaggagtac accaacatct tcctgaagtt cggcagcggc tacgtgagcg   2220

gctggggccg cgtgttccac aagggccgca gcgccctggt gctgcagtac ctgcgcgtgc   2280

ccctggtgga ccgcgccacc tgcctgcgca gcaccaagtt caccatctac aacaacatgt   2340

tctgcgccgg cttccacgag ggcggccgcg acagctgcca gggcgacagc ggcggccccc   2400

acgtgaccga ggtggagggc accagcttcc tgaccggcat catcagctgg ggcgaggagt   2460

gcgccatgaa gggcaagtac ggcatctaca ccaaggtgag ccgctacgtg aactggatca   2520

aggagaagac caagctgacc taatgaaaga tggatttcca aggttaattc attggaattg   2580

aaaattaaca gccccccccc ccccccccct gcagatctag agctcgctga tcagcctcga   2640

ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc   2700

tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc   2760

tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt   2820

gggaagacaa tagcaggcat gctggggacc ggtataactt cgtataatgt atgctatacg   2880

aagttatgca tgcgtcaatt ttacgcagac tatctttcta gggttaagcg cgcttggcgt   2940

aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca   3000

tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat   3060

taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt   3120

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct   3180

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa   3240

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa   3300

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc   3360

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga   3420

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc   3480

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt   3540

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct   3600

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg   3660

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta   3720

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct   3780

acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa   3840

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt   3900

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta   3960

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat   4020

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa   4080

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct   4140

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta   4200

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct   4260

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg   4320

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa   4380

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt   4440

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta   4500

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca   4560

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta   4620

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct   4680

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg   4740

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac   4800

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact   4860

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa   4920

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt   4980

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat   5040

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac      5097


<210>  15
<211>  5097
<212>  DNA
<213>  Artificial

<220>
<223>  PB_micro_T_No_ins_TTRminSerpMVM_FIXco_Padua_bghpA transposon

<400>  15
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc     60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga    120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc    180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc    240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag    300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa    360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac    420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg    480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg    540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg    600

taaaacgacg gccagtgagc gcgcttaacc ctagaaagat aatcatattg tgacgtacgt    660

taaagataat catgcgtaaa attgacgcat gataacttcg tataatgtat gctatacgaa    720

gttatgcggc cgcggtaccg gcgcgccggg ggaggctgct ggtgaatatt aaccaaggtc    780

accccagtta tcggaggagc aaacaggggc taagtccaca cgcgtggtac cgtctgtctg    840

cacatttcgt agagcgagtg ttccgatact ctaatctccc taggcaaggt tcatatttgt    900

gtaggttact tattctcctt ttgttgacta agtcaataat cagaatcagc aggtttggag    960

tcagcttggc agggatcagc agcctgggtt ggaaggaggg ggtataaaag ccccttcacc   1020

aggagaagcc gtcacacaga tccacaagct cctgaagagg taagggttta agggatggtt   1080

ggttggtggg gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca   1140

ggttgggcta gcccaccatg cagcgcgtga acatgatcat ggccgagagc cccggcctga   1200

tcaccatctg cctgctgggc tacctgctga gcgccgagtg caccgtgttc ctggaccacg   1260

agaacgccaa caagatcctg aaccgcccca agcgctacaa cagcggcaag ctggaggagt   1320

tcgtgcaggg caacctggag cgcgagtgca tggaggagaa gtgcagcttc gaggaggccc   1380

gcgaggtgtt cgagaacacc gagcgcacca ccgagttctg gaagcagtac gtggacggcg   1440

accagtgcga gagcaacccc tgcctgaacg gcggcagctg caaggacgac atcaacagct   1500

acgagtgctg gtgccccttc ggcttcgagg gcaagaactg cgagctggac gtgacctgca   1560

acatcaagaa cggccgctgc gagcagttct gcaagaacag cgccgacaac aaggtggtgt   1620

gcagctgcac cgagggctac cgcctggccg agaaccagaa gagctgcgag cccgccgtgc   1680

ccttcccctg cggccgcgtg agcgtgagcc agaccagcaa gctgacccgc gccgaggccg   1740

tgttccccga cgtggactac gtgaacagca ccgaggccga gaccatcctg gacaacatca   1800

cccagagcac ccagagcttc aacgacttca cccgcgtggt gggcggcgag gacgccaagc   1860

ccggccagtt cccctggcag gtggtgctga acggcaaggt ggacgccttc tgcggcggca   1920

gcatcgtgaa cgagaagtgg atcgtgaccg ccgcccactg cgtggagacc ggcgtgaaga   1980

tcaccgtggt ggccggcgag cacaacatcg aggagaccga gcacaccgag cagaagcgca   2040

acgtgatccg catcatcccc caccacaact acaacgccgc catcaacaag tacaaccacg   2100

acatcgccct gctggagctg gacgagcccc tggtgctgaa cagctacgtg acccccatct   2160

gcatcgccga caaggagtac accaacatct tcctgaagtt cggcagcggc tacgtgagcg   2220

gctggggccg cgtgttccac aagggccgca gcgccctggt gctgcagtac ctgcgcgtgc   2280

ccctggtgga ccgcgccacc tgcctgctga gcaccaagtt caccatctac aacaacatgt   2340

tctgcgccgg cttccacgag ggcggccgcg acagctgcca gggcgacagc ggcggccccc   2400

acgtgaccga ggtggagggc accagcttcc tgaccggcat catcagctgg ggcgaggagt   2460

gcgccatgaa gggcaagtac ggcatctaca ccaaggtgag ccgctacgtg aactggatca   2520

aggagaagac caagctgacc taatgaaaga tggatttcca aggttaattc attggaattg   2580

aaaattaaca gccccccccc ccccccccct gcagatctag agctcgctga tcagcctcga   2640

ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc   2700

tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc   2760

tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt   2820

gggaagacaa tagcaggcat gctggggacc ggtataactt cgtataatgt atgctatacg   2880

aagttatgca tgcgtcaatt ttacgcagac tatctttcta gggttaagcg cgcttggcgt   2940

aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca   3000

tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat   3060

taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt   3120

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct   3180

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa   3240

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa   3300

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc   3360

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga   3420

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc   3480

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt   3540

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct   3600

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg   3660

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta   3720

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct   3780

acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa   3840

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt   3900

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta   3960

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat   4020

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa   4080

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct   4140

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta   4200

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct   4260

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg   4320

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa   4380

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt   4440

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta   4500

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca   4560

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta   4620

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct   4680

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg   4740

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac   4800

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact   4860

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa   4920

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt   4980

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat   5040

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac      5097


<210>  16
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  16

Ser Phe Ser Gln Asn Pro Pro Val Leu Thr Arg His Gln Arg 
1               5                   10                  


<210>  17
<211>  6891
<212>  DNA
<213>  Artificial

<220>
<223>  pCDNA3 _CMVBGI _SBMAX_bghpA plasmid

<400>  17
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg     60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg    120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc    180

ttagggttag gcgttttgcg ctgcttcgcg cgtggagcta gttattaata gtaatcaatt    240

acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat    300

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt    360

cccatagtaa cgtcaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa    420

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc    480

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct    540

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag    600

tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt    660

gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac    720

aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc    780

agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc    840

catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc gggaacggtg    900

cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag    960

gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact   1020

ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca   1080

ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa   1140

atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac   1200

aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc   1260

caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg   1320

gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga ttcgaacatc   1380

gatgccgcca ccatgggaaa atcaaaagaa atcagccaag acctcagaaa aagaattgta   1440

gacctccaca agtctggttc atccttggga gcaatttccc gacgcctggc ggtaccacgt   1500

tcatctgtac aaacaatagt acgcaagtat aaacaccatg ggaccacgca gccgtcatac   1560

cgctcaggaa ggagacgcgt tctgtctcct agagatgaac gtactttggt gcgaaaagtg   1620

caaatcaatc ccagaacaac agcaaaggac cttgtgaaga tgctggagga aacaggtaca   1680

aaagtatcta tatccacagt aaaacgagtc ctatatcgac ataacctgaa aggccactca   1740

gcaaggaaga agccactgct ccaaaaccga cataagaaag ccagactacg gtttgcaact   1800

gcacatgggg acaaagatct aactttttgg agaaatgtcc tctggtctga tgaaacaaaa   1860

atagaactgt ttggccataa tgaccatcgt tatgtttgga ggaagaaggg ggaggcttgc   1920

aagccgaaga acaccatccc aaccgtgaag cacgggggtg gcagcatcat gttgtggggg   1980

tgctttgctg caggagggac tggtaaactt gtccgaatag aaggcatcat ggacgcggtg   2040

cagtatgtgg atatattgaa gcaacatctc aagacatcag tcaggaagtt aaagcttggt   2100

cgcaaatggg tcttccaaca cgacaatgac cccaagcata cttccaaagt tgtggcaaaa   2160

tggcttaagg acaacaaagt caaggtattg gactggccat cacaaagccc tgacctcaat   2220

cctatagaaa atttgtgggc agaactgaaa aagcgtgtgc gagcaaggag gcctacaaac   2280

ctgactcagt tacaccagct ctgtcaggag gaatgggcca aaattcaccc aaattattgt   2340

gggaagcttg tggaaggcta cccgaaacgt ttgacccaag ttaaacaatt taaaggcaat   2400

gctaccaaat actagctcga gcatgcatct agagggccct attctatagt gtcacctaaa   2460

tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg   2520

cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata   2580

aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt   2640

ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt   2700

gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc   2760

gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac   2820

acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt   2880

cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc   2940

tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc   3000

gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact   3060

cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg   3120

gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc   3180

gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc   3240

aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc   3300

aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt   3360

cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc   3420

ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct   3480

attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg   3540

agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat   3600

tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta   3660

tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca   3720

ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga   3780

cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga   3840

cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct   3900

cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg   3960

gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga   4020

gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca   4080

tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga   4140

ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg   4200

cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc   4260

gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt   4320

gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga   4380

gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca   4440

tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc   4500

cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac   4560

cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc   4620

acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta   4680

tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag   4740

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc   4800

ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc   4860

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa   4920

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg   4980

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg   5040

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag   5100

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac   5160

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga   5220

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt   5280

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc   5340

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc   5400

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta   5460

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat   5520

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca   5580

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct   5640

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt   5700

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct   5760

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc   5820

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa   5880

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta   5940

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc   6000

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat   6060

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta   6120

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt   6180

aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt   6240

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg   6300

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc   6360

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc   6420

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg   6480

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga   6540

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta   6600

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct   6660

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag   6720

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga   6780

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat   6840

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c            6891


<210>  18
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  18
gaaggtgaag gtcggagtc                                                  19


<210>  19
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  19
gaagatggtg atgggatttc                                                 20


<210>  20
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  20
tgatgctatt gctttatttg taacc                                           25


<210>  21
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  21
cctgaacctg aaacataaaa tga                                             23


<210>  22
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  probe

<400>  22
agctgcaata aacaagttaa caacaacaat tgca                                 34


<210>  23
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Fragment codon-optimized human coagulation factor IX with Padua 
       mutation

<400>  23
cctgcctgct gagcaccaa                                                  19


<210>  24
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Fragment codon-optimized human coagulation factor IX

<400>  24
cctgcctgcg cagcaccaa                                                  19


<210>  25
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  25
aacaggggct aagtccacac                                                 20


<210>  26
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  26
gagcgagtgt tccgatactc t                                               21


<210>  27
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  27
atcaagaagg tggtgaagca ggca                                            24


<210>  28
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  28
tggaagagtg ggagttgctg ttga                                            24


<210>  29
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  IR micro

<400>  29
ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg     60

acgcatg                                                               67


<210>  30
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  IR micro

<400>  30
gcatgcgtca attttacgca gactatcttt ctagggttaa                           40


