                         SEQUENCE LISTING

<110>  Galen, James E
       Wang, Jin-Yuang
 
<120>  BACTERIAL LIVE VECTOR VACCINES EXPRESSING 
       CHROMOSOMALLY-INTEGRATED FOREIGN ANTIGENS

<130>  61045

<150>  US 61/713,806
<151>  2012-10-15

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5guaBA-for

<400>  1
gaattctagc tgctcatact tctgctgca                                         29


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5guaBA-rev

<400>  2
gctagccaat tggggcaata tctcacctgg                                        30


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3guaBA-for

<400>  3
ggatccacta gtgtcgataa cccttcctgt gt                                     32


<210>  4
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3guaBA-rev

<400>  4
ctcgagacag cacctacaag tctggcatg                                         29


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer guaBA PCR-for

<400>  5
gcgctgacca ccggaatacg gctg                                              24


<210>  6
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer guaBA PCR-rev

<400>  6
catggcatgg atgaggcaac cgcgaagc                                          28


<210>  7
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5htrA-for

<400>  7
gaattcgtac cttcaatcag gcgttactgg aagatg                                 36


<210>  8
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5htrA-rev

<400>  8
gctagccaat tgcgattaac aggtaacgca aaattgctgt gtacgtcag                   49


<210>  9
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3htrA-for

<400>  9
ggatccacta gtctgcgtaa gattctcgac agcaagccgt cggt                        44


<210>  10
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3htrA-rev

<400>  10
ctcgagccag catcatttcg gcagtcatac acaccagttc gc                          42


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer htrA PCR-for

<400>  11
gtgtcgccga tcttgaagac gcggtagag                                         29


<210>  12
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer htrA PCR-rev

<400>  12
ctatcgacgc caagctggcc gctgtcgac                                         29


<210>  13
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5clyA-for

<400>  13
tagtaatgag aattcgctgg tattgatcgg ctctccggta gagattagcg a                51


<210>  14
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5clyA-rev

<400>  14
gctagccaat tgtgcctctt taaatatata aattgcaatt aagtacctg                   49


<210>  15
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3clyA-for

<400>  15
ggatccacta gtgatacatt ttcattcgat ctgtgtactt ttaacgcccg atagcg           56


<210>  16
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3clyA-rev

<400>  16
tgatagtaac tcgagacaat ccataagaaa ggtcaggcac actgggaagg cgacatc          57


<210>  17
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer clyA PCR-for

<400>  17
catgatggta tccagtatgg cacaagc                                           27


<210>  18
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer clyA PCR-rev

<400>  18
gtaatcgaca acatgctaca tccatcg                                           27


<210>  19
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5FRT-aph-for

<400>  19
gaattcgcta gcgctggagc tgcttcgaag ttc                                    33


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3FRT-aph-rev

<400>  20
ctcgagttcc ggggatccgt cgacctgcag ttc                                    33


<210>  21
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5gfpuv

<400>  21
caattgtgtg gtagcacaga ataatgaaaa gt                                     32


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3gfpuv

<400>  22
gctagctcat tatttgtaga gctcatccat                                        30


<210>  23
<211>  996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimized gene cassette tcdA-14CBD/A

<400>  23
tagtaatgaa tggctagcac cggctatacg agcatcaacg ggaagcattt ttatttcaat       60

accgacggca ttatgcagat cggggtgttc aaagggccca acggtttcga atactttgcg      120

ccggccaaca cggatgcgaa taacatcgaa ggtcaagcca tcctctacca gaacaaattc      180

ctgaccctca atggcaagaa atattacttc gggtccgaca gcaaggctgt cacgggtctg      240

cgcacgattg atgggaaaaa gtattacttc aacaccaata ctgccgtggc ggtcaccgga      300

tggcaaacga tcaacggcaa gaaatactac ttcaatacca acacgtccat tgcctcgacc      360

gggtatacga tcatttccgg caaacacttc tacttcaaca ccgacgggat catgcaaatc      420

ggtgtgttta aagggcccga tggcttcgag tactttgccc cggcgaatac ggacgccaat      480

aacattgaag gtcaggcaat tcggtatcag aatcgttttc tctatctgca tgacaacatc      540

tactatttcg gtaataacag caaggcggcc accgggtggg tcacgattga tggcaaccgg      600

tattacttcg agcccaatac ggcgatgggg gcgaatggtt ataaaacgat cgacaacaag      660

aatttctact ttcgcaacgg gctcccgcag attggcgtgt tcaaagggtc caacggcttt      720

gagtacttcg cccccgcgaa tacggatgcc aacaatatcg agggccaagc gattcggtat      780

caaaaccgct tcctccacct gctcgggaaa atctattact tcggcaataa ctcgaaagcc      840

gtcacgggtt ggcaaacgat taatggcaaa gtgtattact tcatgccgga tactgcaatg      900

gcagccgctg gtggattatt cgaaattgac ggcgtcatct atttctttgg cgtggatggg      960

gtcaaagccc cggggatcta tggctaatga cctagg                                996


<210>  24
<211>  1629
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimized gene cassette TcdB CBD/B

<400>  24
tagtaatgaa tggctagcgg cctgatctat attaacgatt cactgtacta tttcaagccg       60

cccgtcaata acctcatcac cgggtttgtg acggtcggcg acgataaata ctacttcaat      120

ccgatcaacg gcggagccgc gagcattggg gagaccatca ttgatgacaa gaattattac      180

ttcaaccaga gtggcgtgct gcagacgggg gtcttcagta ccgaggacgg ctttaaatac      240

ttcgcccccg cgaatacgct cgatgagaac ctggaagggg aggcgattga ctttaccggc      300

aagctgatca ttgacgagaa catctactat ttcgatgaca attaccgcgg ggccgtggaa      360

tggaaggagc tcgatggcga gatgcactat tttagcccgg agacggggaa agctttcaaa      420

ggcctgaatc aaattgggga ctacaagtat tacttcaact ctgacggtgt gatgcagaag      480

gggttcgtga gtatcaatga caacaagcat tatttcgacg attctggcgt catgaaagtg      540

gggtacaccg agatcgatgg caagcacttc tactttgccg agaatggtga gatgcaaatc      600

ggcgtgttca atacggaaga cgggtttaag tatttcgcgc atcataacga ggatctcggc      660

aatgaagagg gcgaagagat ctcatattcc ggaatcctca acttcaataa caagatttac      720

tactttgatg actcatttac cgccgtggtc ggctggaagg acctcgagga tgggtcaaag      780

tactatttcg acgaggatac ggcggaggcc tacatcggcc tgtcattaat caacgacggc      840

cagtattact ttaatgatga cggcatcatg caggtcgggt ttgtcaccat caacgataaa      900

gtcttctact tctctgactc tggcattatc gagagcgggg tgcagaacat cgacgataac      960

tatttctaca tcgatgacaa tggcattgtc cagatcggcg tgttcgatac gtcagacggt     1020

tataagtatt ttgcgcccgc caacaccgtc aacgataata tctacggtca agctgttgaa     1080

tatagtggtt tggtccgtgt cggtgaagac gtgtactatt tcggcgagac gtacacaatt     1140

gagacgggct ggatctatga tatggagaac gagagtgaca agtactattt caatcctgag     1200

accaagaaag catgcaaggg gatcaacctg atcgatgaca tcaagtacta tttcgacgag     1260

aagggcatta tgcgcacggg gcttatctca tttgagaata acaattatta cttcaatgag     1320

aacggggaaa tgcagtttgg gtacatcaat attgaggaca agatgttcta ttttggcgag     1380

gatggcgtca tgcagatcgg ggtgttcaac accccagatg gtttcaagta tttcgcgcat     1440

cagaatacgc tggatgagaa cttcgagggc gaatcaatca actataccgg gtggctggac     1500

ctcgatgaga agcgctacta tttcacggac gaatacattg cggccaccgg ctcagtcatc     1560

attgatggcg aggaatacta tttcgaccct gatacggcgc agctggtgat cagtgagtaa     1620

tgacctagg                                                             1629


<210>  25
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5rpoS-for

<400>  25
aagcttgaat tccgtattct gagggctcag gtgaacaaag tgc                         43


<210>  26
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5rpoS-rev

<400>  26
cctaggcaat tgacccgtga tcccttgacg gaactagcaa gtc                         43


<210>  27
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3rpoS-for

<400>  27
ggatccggtt cggtatcgcg ccaggtatac agacaatgc                              39


<210>  28
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3rpoS-rev

<400>  28
ctcgagccgg aagtgcaggc ggtaaacgct atgtacac                               38


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer rpoS PCR-for

<400>  29
atgcagcaca gcaaggagtt gtgacca                                           27


<210>  30
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer rpoS PCR-rev

<400>  30
ggtgcgtatc gataaggtct cttaccacag c                                      31


<210>  31
<211>  3290
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5guaB-PompC-14CBDA-FRT-3guaA gene cassette

<400>  31
gcgctgacca ccggaatacg gctggcaaaa atagcccgcg ccacgcgttc gtcgttaaaa       60

ctccacaaat cttccagcga accgccgccg cgtccgacga tcagcacgtc gcactcgccg      120

cgcgtgtttg ccagttcgat agcacgaacg atctgccccg gcgcatcgtc gccctgtacc      180

gcggttggat agataataac gggcagggat gggtcacgac gcttcaacac gtggaggata      240

tcgtgcagcg ccgcgccggt tttcgaggtg atcaccccaa cgcagtgggc cggggagggc      300

aacggctgtt tatgctgctg atcgaacaag ccttcggcat ggagtttggc ttttagctgc      360

tcatacttct gctgcaacaa gccttcgcca gccggctgca tactttcggc gatgatttga      420

taatcgccgc gcggctcgta cagcgtaatg ttggcgcgta ccagcacctg ctgcccgtgc      480

tgcgggcgga acgtcacccg gcggttgctg ttgcggaaca tcgcacagcg cacctgagcg      540

gtatcgtctt tgagcgtaaa gtaccagtgg cccgacgcag gctgcgtgaa attagaaatc      600

tcgccgctga tccatacctg tcccatctcc tgttctaaca gcagacgaac cgtctggtta      660

aggcggctta cggtaaaaat tgaggaagtt tgagaggata acatgtgagc gggatcaaat      720

tctaaatcag caggttattc aatcgatagt aacctgctca cgggggatcg caagcactat      780

ttgcaaaaaa atgtagatgc aaccgattac gttctgtata atgccgcggc aatatttatt      840

aacctcccag gtgagatatt gccccaattc gtcaattctg tggtagcaca gaataatgaa      900

aagtgtgtaa agaagggtaa aaaaaaccga atgcgaggca tccggttgaa ataggggtaa      960

acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag cgggagttat     1020

tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact taagaataag     1080

ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt cgccattccg     1140

caataatctt aaaaagttcc cttgcattta cattttgaaa catctatagc gataaatgaa     1200

acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt tggggagaat     1260

ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa aagcaaataa     1320

aggcatataa cagatcgatc ttaaacatcc acaggaggat gggatccaaa ataaggagga     1380

aaaaaaaatg actagcaccg gctatacgag catcaacggg aagcattttt atttcaatac     1440

cgacggcatt atgcagatcg gggtgttcaa agggcccaac ggtttcgaat actttgcgcc     1500

ggccaacacg gatgcgaata acatcgaagg tcaagccatc ctctaccaga acaaattcct     1560

gaccctcaat ggcaagaaat attacttcgg gtccgacagc aaggctgtca cgggtctgcg     1620

cacgattgat gggaaaaagt attacttcaa caccaatact gccgtggcgg tcaccggatg     1680

gcaaacgatc aacggcaaga aatactactt caataccaac acgtccattg cctcgaccgg     1740

gtatacgatc atttccggca aacacttcta cttcaacacc gacgggatca tgcaaatcgg     1800

tgtgtttaaa gggcccgatg gcttcgagta ctttgccccg gcgaatacgg acgccaataa     1860

cattgaaggt caggcaattc ggtatcagaa tcgttttctc tatctgcatg acaacatcta     1920

ctatttcggt aataacagca aggcggccac cgggtgggtc acgattgatg gcaaccggta     1980

ttacttcgag cccaatacgg cgatgggggc gaatggttat aaaacgatcg acaacaagaa     2040

tttctacttt cgcaacgggc tcccgcagat tggcgtgttc aaagggtcca acggctttga     2100

gtacttcgcc cccgcgaata cggatgccaa caatatcgag ggccaagcga ttcggtatca     2160

aaaccgcttc ctccacctgc tcgggaaaat ctattacttc ggcaataact cgaaagccgt     2220

cacgggttgg caaacgatta atggcaaagt gtattacttc atgccggata ctgcaatggc     2280

agccgctggt ggattattcg aaattgacgg cgtcatctat ttctttggcg tggatggggt     2340

caaagccccg gggatctatg gctaatgacc tagctgataa cctagcccgc ctaatgagcg     2400

ggcttttttt tctcggccta gcgctggagc tgcttcgaag ttcctatact ttctagagaa     2460

taggaacttc gaactgcagg tcgacggatc cactagtgtc gataaccctt cctgtgtttt     2520

catgaacagg taaaagtgaa tttaaccctc tgtttttgca gagggttttt atttatgtgc     2580

atagatgcat tttctgtgta gtgcacagct tctggcggca aagcactgca tcgacacaat     2640

agtgggtttc actggcagca cacaatcaaa tctgacagtc ggcttcgagc gaggtgcgga     2700

cattggtagc ttcaacaaca tgactggcat tgaggtgtat taatcaacgg gaagcaggtc     2760

agtttactcc gccagcccga cttttacgaa agggctacca tgactacgcg attttttgat     2820

tagcttgcca acgtataatc ctctgatttc tggtgatgcg attcgtccaa caaattctga     2880

acggttggtt tcgggggggg gatgaccaat tcccgggtaa agtattgctg agagcctgcc     2940

ttcaaccagc actgtatctc gtaaacttcc atcaccaaac cgccgtaggt ggcgtatgca     3000

aattgtaggt tttcatcttt tggtactttt gcccagacgc cacgcgtagc ttcatataat     3060

gcaagtgcac tcatgccaga cttgtaggtg ctgtttagca aaaaagcgag cccggcatgt     3120

tcaggtgcga tttccgtctc ttgcttaaga agaagatgat tgagctcatc cagcgtgatt     3180

cgccccataa gggagctgct tcctcgtact ttgttggtca gttctccaac acccaagaga     3240

tcaatacatg ttgcttcaac aagcttcgcg gttgcctcat ccatgccatg                3290


<210>  32
<211>  3229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5htrA-PompC-14CBDA-FRT-3htrA gene cassette

<400>  32
gtgtcgccga tcttgaagac gcggtagaga aaagaatctt tagcgttgag cagctttatc       60

accatttata tcacgcgtgg ggccaccatg agaaggattc gctgtttgag ctggtggtag      120

gaaatgcgtg ggaaaaatca cgcgccaata cattaagccg cagtaccgaa gatcagtttt      180

ttatgtattt acgggtaaat acattaaata aactggtgcc ctatgccgct cagcgtttta      240

ttgataattt gccgcagatt tttgccggta ccttcaatca ggcgttactg gaagatgcca      300

gcggttttag ccgcctgctt gaactctata agaatgtggc ggttgaacat gtgtttagcc      360

atccggatgt agaacagctt gaactacagg gataccgggt gatcagcggg ttattagata      420

tctatcagcc gctattaagc ttgtcgctta acgactttcg cgagctggtg gaaaaagaac      480

ggttgaaacg cttccctata gaatcgcgct tatttcagaa actttctacg cgccatcgtt      540

tggcctacgt ggaagtcgtc agtaaattac ccacggattc ggcggagtac ccggtactgg      600

aatattatta tcgctgtcgg ttgattcagg attatatcag cgggatgact gacctttacg      660

catgggatga atatcggcgt ttgatggcgg tcgaacagta aatggacttt tgtaaagatg      720

gacaataaat ttttactttt tccagaaact ttattccgga acttcgcgtt ataaaatgaa      780

tctgacgtac acagcaattt tgcgttacct gttaatcgca attcgtcaat tctgtggtag      840

cacagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa ccgaatgcga ggcatccggt      900

tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa gttaatgatg      960

atagcgggag ttattctagt tgcgagtgaa ggttttgttt tgacattcag tgctgtcaaa     1020

tacttaagaa taagttattg attttaacct tgaattatta ttgcttgatg ttaggtgctt     1080

atttcgccat tccgcaataa tcttaaaaag ttcccttgca tttacatttt gaaacatcta     1140

tagcgataaa tgaaacatct taaaagtttt agtatcatat tcgtgttgga ttattctgca     1200

tttttgggga gaatggactt gccgactgat taatgagggt taatcagtat gcagtggcat     1260

aaaaaagcaa ataaaggcat ataacagatc gatcttaaac atccacagga ggatgggatc     1320

caaaataagg aggaaaaaaa aatgactagc accggctata cgagcatcaa cgggaagcat     1380

ttttatttca ataccgacgg cattatgcag atcggggtgt tcaaagggcc caacggtttc     1440

gaatactttg cgccggccaa cacggatgcg aataacatcg aaggtcaagc catcctctac     1500

cagaacaaat tcctgaccct caatggcaag aaatattact tcgggtccga cagcaaggct     1560

gtcacgggtc tgcgcacgat tgatgggaaa aagtattact tcaacaccaa tactgccgtg     1620

gcggtcaccg gatggcaaac gatcaacggc aagaaatact acttcaatac caacacgtcc     1680

attgcctcga ccgggtatac gatcatttcc ggcaaacact tctacttcaa caccgacggg     1740

atcatgcaaa tcggtgtgtt taaagggccc gatggcttcg agtactttgc cccggcgaat     1800

acggacgcca ataacattga aggtcaggca attcggtatc agaatcgttt tctctatctg     1860

catgacaaca tctactattt cggtaataac agcaaggcgg ccaccgggtg ggtcacgatt     1920

gatggcaacc ggtattactt cgagcccaat acggcgatgg gggcgaatgg ttataaaacg     1980

atcgacaaca agaatttcta ctttcgcaac gggctcccgc agattggcgt gttcaaaggg     2040

tccaacggct ttgagtactt cgcccccgcg aatacggatg ccaacaatat cgagggccaa     2100

gcgattcggt atcaaaaccg cttcctccac ctgctcggga aaatctatta cttcggcaat     2160

aactcgaaag ccgtcacggg ttggcaaacg attaatggca aagtgtatta cttcatgccg     2220

gatactgcaa tggcagccgc tggtggatta ttcgaaattg acggcgtcat ctatttcttt     2280

ggcgtggatg gggtcaaagc cccggggatc tatggctaat gacctagctg ataacctagc     2340

ccgcctaatg agcgggcttt tttttctcgg cctagcgctg gagctgcttc gaagttccta     2400

tactttctag agaataggaa cttcgaactg caggtcgacg gatccactag tctgcgtaag     2460

attctcgaca gcaagccgtc ggtgctggcg ctgaatattc agcgtggtga tagttctatt     2520

tatttgctga tgcagtaatc acctttgtcc cccttccacc acggaagggg gcaacacttt     2580

tctgtgaaac tccccacaac tccatacttc tttgcaccgt tttgtgcatt tgcacaatgt     2640

cgaggcctgt catcttcctt atgcttgtgc tctgctcaca ggagggattt atggctggct     2700

ggcatcttga taccaaaatg gcgcaggata tcgtggcgcg cactatgcgc atcatcgata     2760

ccaatatcaa cgtaatggat gcccgcgggc gtattatcgg cagcggcgat cgggaacgta     2820

ttggtgaatt gcacgaaggc gcgttgttag tgctgtcaca gggccgggtt gtggatatcg     2880

acaacgccgt ggcgcgacac ctgcacgggg tgcgtcaggg gattaatctt cccttacgcc     2940

ttgagggcga aattgttggc gtgatcggtc tcaccggcga accagagcat ctgcgtaaat     3000

atggcgaact ggtgtgtatg actgccgaaa tgatgctgga acagtcgcgg ttaatgcacc     3060

ttttggcgca ggatagccgt ttgcgtgaag agctggtgat gaacctgatt caggccgaag     3120

aaaatacgcc ggcgctggtg gaatgggcac agcgtttagg gatcgatttg aaccagccgc     3180

gtgtggcggc ggtggtggaa gtcgacagcg gccagcttgg cgtcgatag                 3229


<210>  33
<211>  2982
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5rpoS-PompC-14CBDA-FRT-3rpoS gene cassette

<400>  33
atgcagcaca gcaaggagtt gtgaccaggt ctgcacaaaa ttccaccgtt gcagttgcgt       60

ctcaaccaac aattacgtat tctgagggct caggtgaaca aagtgctaac aaaatgttgc      120

caaacaacaa gcctgctggg acggttgtca cagcgcctgt aacggcacca acggtaagca      180

cgaccgaacc aaatgcaagc agtacgtcaa ccagcgcgcc gatttccgca tggcgctggc      240

cgacggatgg caaagtgatc gaaaactttg gcgcttccga agggggcaat aaagggatcg      300

acattgcagg cagtaaggga caggctatcg tcgcaaccgc tgatgggcgc gtcgtatatg      360

ccggtaacgc actgcgtggt tacggtaatc ttattatcat caaacataac gatgattacc      420

tgagtgccta cgcccataat gatacgatgc tggtccggga acaacaggaa gttaaggcgg      480

ggcaaaaaat cgctactatg ggtagcaccg gcaccagctc tacacgcttg cattttgaaa      540

ttcgttacaa ggggaaatcc gtaaacccgc tgcgttattt accgcagcga taaagcggcg      600

gaaccaggct ttgacttgct agttccgtca agggatcacg ggtcaattcg tcaattctgt      660

ggtagcacag aataatgaaa agtgtgtaaa gaagggtaaa aaaaaccgaa tgcgaggcat      720

ccggttgaaa taggggtaaa cagacattca gaaatgaatg acggtaataa ataaagttaa      780

tgatgatagc gggagttatt ctagttgcga gtgaaggttt tgttttgaca ttcagtgctg      840

tcaaatactt aagaataagt tattgatttt aaccttgaat tattattgct tgatgttagg      900

tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac attttgaaac      960

atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt     1020

ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt     1080

ggcataaaaa agcaaataaa ggcatataac agatcgatct taaacatcca caggaggatg     1140

ggatccaaaa taaggaggaa aaaaaaatga ctagcaccgg ctatacgagc atcaacggga     1200

agcattttta tttcaatacc gacggcatta tgcagatcgg ggtgttcaaa gggcccaacg     1260

gtttcgaata ctttgcgccg gccaacacgg atgcgaataa catcgaaggt caagccatcc     1320

tctaccagaa caaattcctg accctcaatg gcaagaaata ttacttcggg tccgacagca     1380

aggctgtcac gggtctgcgc acgattgatg ggaaaaagta ttacttcaac accaatactg     1440

ccgtggcggt caccggatgg caaacgatca acggcaagaa atactacttc aataccaaca     1500

cgtccattgc ctcgaccggg tatacgatca tttccggcaa acacttctac ttcaacaccg     1560

acgggatcat gcaaatcggt gtgtttaaag ggcccgatgg cttcgagtac tttgccccgg     1620

cgaatacgga cgccaataac attgaaggtc aggcaattcg gtatcagaat cgttttctct     1680

atctgcatga caacatctac tatttcggta ataacagcaa ggcggccacc gggtgggtca     1740

cgattgatgg caaccggtat tacttcgagc ccaatacggc gatgggggcg aatggttata     1800

aaacgatcga caacaagaat ttctactttc gcaacgggct cccgcagatt ggcgtgttca     1860

aagggtccaa cggctttgag tacttcgccc ccgcgaatac ggatgccaac aatatcgagg     1920

gccaagcgat tcggtatcaa aaccgcttcc tccacctgct cgggaaaatc tattacttcg     1980

gcaataactc gaaagccgtc acgggttggc aaacgattaa tggcaaagtg tattacttca     2040

tgccggatac tgcaatggca gccgctggtg gattattcga aattgacggc gtcatctatt     2100

tctttggcgt ggatggggtc aaagccccgg ggatctatgg ctaatgacct agctgataac     2160

ctagcccgcc taatgagcgg gctttttttt ctcggcctag cgacgaattg cctagcgctg     2220

gagctgcttc gaagttccta tactttctag agaataggaa cttcgaactg caggtcgacg     2280

gatccggttc ggtatcgcgc caggtataca gacaatgctg acactgatat accgtccaga     2340

ctccttttac cggcgaagtc gccatgagtt caatatgtgc atcggcacag cgtggacaaa     2400

tcatcggtta ctccttattt acggttggcc agcatggcgg tcagcttttc agcccaggct     2460

ttagtttccg gtaaatccac aaccggctgg ctatagtgac cacggttatc tggcgcaacc     2520

ggcgtggtag cgtcaatgat cagtttgtcg gtaatccccg ccgggcttga acccgggtcc     2580

agttccagga cggacatatt cggtagctgt accagatcgc ctgccggatt cactttcgac     2640

gacaacgccc acatcacctg tggcagatta aatggatcga catcttcatc aaccatgatc     2700

accatcttca catatcccag accgtgcggc gtcgtcatcg cgcgcaggcc caccgcacgg     2760

gcaaagccgc cgtagcgttt tttggtcgag atgatggcca gcaagccgtg ggtgtacata     2820

gcgtttaccg cctgcacttc cggaaattcg gctttcagct gttgatacag tggcacacag     2880

gtcgccggcc ccatcaggta gtcgatttcc gtccacggca tccccaaata gagcgattca     2940

aaaatgggtt tgctgtggta agagacctta tcgatacgca cc                        2982


<210>  34
<211>  325
<212>  PRT
<213>  Clostridium difficile

<400>  34

Met Thr Ser Thr Gly Tyr Thr Ser Ile Asn Gly Lys His Phe Tyr Phe 
1               5                   10                  15      


Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly 
            20                  25                  30          


Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly 
        35                  40                  45              


Gln Ala Ile Leu Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys 
    50                  55                  60                  


Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Leu Arg Thr Ile 
65                  70                  75                  80  


Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Val Ala Val Thr 
                85                  90                  95      


Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr 
            100                 105                 110         


Ser Ile Ala Ser Thr Gly Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr 
        115                 120                 125             


Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asp 
    130                 135                 140                 


Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu 
145                 150                 155                 160 


Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu Tyr Leu His Asp Asn 
                165                 170                 175     


Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Ala Thr Gly Trp Val Thr 
            180                 185                 190         


Ile Asp Gly Asn Arg Tyr Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala 
        195                 200                 205             


Asn Gly Tyr Lys Thr Ile Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly 
    210                 215                 220                 


Leu Pro Gln Ile Gly Val Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe 
225                 230                 235                 240 


Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg 
                245                 250                 255     


Tyr Gln Asn Arg Phe Leu His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly 
            260                 265                 270         


Asn Asn Ser Lys Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Val 
        275                 280                 285             


Tyr Tyr Phe Met Pro Asp Thr Ala Met Ala Ala Ala Gly Gly Leu Phe 
    290                 295                 300                 


Glu Ile Asp Gly Val Ile Tyr Phe Phe Gly Val Asp Gly Val Lys Ala 
305                 310                 315                 320 


Pro Gly Ile Tyr Gly 
                325 


<210>  35
<211>  1542
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssb region of chromosomal deletion

<400>  35
ttcggcggat cggagagatc gcagacttcg ccgtcaatac gggcgcgaat gtaaccctgg       60

cttgccagat tttccagcgt tttggtgtgt tcgcctttac gctctttaat aatcggcgcc      120

agcagcatca ggcgtttgcc ttccggctgc gacagcacgt tatcgaccat ctggctaacg      180

gtttgcgccg ccagcggcac gtcatggtcc ggacaacgcg gctcgcccac gcgggcaaac      240

agcaggcgca ggtagtcgtg gatctcggta atagtaccca ccgtagagcg cgggttgtgc      300

gatgtcgatt tctgttcaat tgagatcgcg ggcgatagcc cctcaatatg gtcgacatcc      360

ggtttttcca tgagcgacaa aaactgccgc gcgtaagcgg agagcgattc aacgtaacga      420

cgctgccctt cggcatacag agtgtcgaaa gccagtgagg atttgcctga acccgaaagc      480

ccggtcacga caatcagttt gtcgcggggg atgacgaggt taatattttt gagattatgg      540

gtgcgggcgc cccgaacttc gatcttatcc attcaccttt cccggtagag actcggatgc      600

ctggtttgtt tgaaggacaa acggcagaaa cggctaatta tgacacaatt taacctgttt      660

gaatatacag tagctagcgt gtaggctgga gctgcttcga agttcctata ctttctagag      720

aataggaact tcggaatagg aactaaggag gatattcata tgcattttcg ctatagttct      780

cgtctgctga aatgcctggt gtaaaccagg cattttatta cctggtatta ttgtcttagt      840

atccctttca aaaaagagaa gcgcatatct cacggaatga cttacggaaa aatgtcgctt      900

atcgcctctg gccgactcgc ctcggcacat cattatccag ccgaagttca taaatgtact      960

gcaataaccc ggattgtctt aaatatgaaa gagaaatctc atctgcaaaa tatataattt     1020

atagccattt tttgacaaca aaaagatatt ataaaataac ggtagagaat ggtcggtatt     1080

atcaatagtt aattaaatgc ttgctttagc ttgtgatgag ctcaaatatg atatgtgtat     1140

cttgctttat ttttaattgc tgaagataaa attgttactt atagctggct ttatataaaa     1200

aatggtttta tttgtgtatt tttttacaca attctgattt tttactcccc acttattata     1260

ttttcaatga tttaagttat attgaagtcc ctatgaccct atgttttaat tgtgaaaatt     1320

cattttatcc tctggaggca aatttattaa atacgtatgg ttataacgcg tattaaaaag     1380

aaatatgtcc ttaaaatgat ttagagtttc aatgattagc taacaaatct atttatcggc     1440

gggtggtttt aatttgctga tgactatttt ttatttatga gctgagagct tatgcgatac     1500

ctattattgg ggaagatata gttagtgcaa taattgatgt ct                        1542


<210>  36
<211>  8045
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PompC-clyA-cbd/b cassette

<400>  36
gaattctgtg gtagcacaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaaccgaat       60

gcgaggcatc cggttgaaat aggggtaaac agacattcag aaatgaatga cggtaataaa      120

taaagttaat gatgatagcg ggagttattc tagttgcgag tgaaggtttt gttttgacat      180

tcagtgctgt caaatactta agaataagtt attgatttta accttgaatt attattgctt      240

gatgttaggt gcttatttcg ccattccgca ataatcttaa aaagttccct tgcatttaca      300

ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt      360

tggattattc tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca      420

gtatgcagtg gcataaaaaa gcaaataaag gcatataaca gatcgatctt aaacatccac      480

aggaggatgg gatccaaaat aaggaggaaa aaaaaatgac tagtattttt gcagaacaaa      540

ctgtagaggt agttaaaagc gcgatcgaaa ccgcagatgg ggcattagat ctttataaca      600

aatacctcga ccaggtcatc ccctggaaga cctttgatga aaccataaaa gagttaagcc      660

gttttaaaca ggagtactcg caggaagctt ctgttttagt tggtgatatt aaagttttgc      720

ttatggacag ccaggacaag tattttgaag cgacacaaac tgtttatgaa tggtgtggtg      780

tcgtgacgca attactctca gcgtatattt tactatttga tgaatataat gagaaaaaag      840

catcagccca gaaagacatt ctcattagga tattagatga tggtgtcaag aaactgaatg      900

aagcgcaaaa atctctcctg acaagttcac aaagtttcaa caacgcttcc ggaaaactgc      960

tggcattaga tagccagtta actaatgatt tttcggaaaa aagtagttat ttccagtcac     1020

aggtggatag aattcgtaag gaagcttatg ccggtgctgc agccggcata gtcgccggtc     1080

cgtttggatt aattatttcc tattctattg ctgcgggcgt gattgaaggg aaattgattc     1140

cagaattgaa taacaggcta aaaacagtgc aaaatttctt tactagctta tcagctacag     1200

tgaaacaagc gaataaagat atcgatgcgg caaaattgaa attagccact gaaatagcag     1260

caattgggga gataaaaacg gaaaccgaaa caaccagatt ctacgttgat tatgatgatt     1320

taatgctttc tttattaaaa ggagctgcaa agaaaatgat taacacctgt aatgaatacc     1380

aacaacgtca tggtaagaag acgcttttcg aggttcctga cgtcgctagc ggcctgatct     1440

atattaacga ttcactgtac tatttcaagc cgcccgtcaa taacctcatc accgggtttg     1500

tgacggtcgg cgacgataaa tactacttca atccgatcaa cggcggagcc gcgagcattg     1560

gggagaccat cattgatgac aagaattatt acttcaacca gagtggcgtg ctgcagacgg     1620

gggtcttcag taccgaggac ggctttaaat acttcgcccc cgcgaatacg ctcgatgaga     1680

acctggaagg ggaggcgatt gactttaccg gcaagctgat cattgacgag aacatctact     1740

atttcgatga caattaccgc ggggccgtgg aatggaagga gctcgatggc gagatgcact     1800

attttagccc ggagacgggg aaagctttca aaggcctgaa tcaaattggg gactacaagt     1860

attacttcaa ctctgacggt gtgatgcaga aggggttcgt gagtatcaat gacaacaagc     1920

attatttcga cgattctggc gtcatgaaag tggggtacac cgagatcgat ggcaagcact     1980

tctactttgc cgagaatggt gagatgcaaa tcggcgtgtt caatacggaa gacgggttta     2040

agtatttcgc gcatcataac gaggatctcg gcaatgaaga gggcgaagag atctcatatt     2100

ccggaatcct caacttcaat aacaagattt actactttga tgactcattt accgccgtgg     2160

tcggctggaa ggacctcgag gatgggtcaa agtactattt cgacgaggat acggcggagg     2220

cctacatcgg cctgtcatta atcaacgacg gccagtatta ctttaatgat gacggcatca     2280

tgcaggtcgg gtttgtcacc atcaacgata aagtcttcta cttctctgac tctggcatta     2340

tcgagagcgg ggtgcagaac atcgacgata actatttcta catcgatgac aatggcattg     2400

tccagatcgg cgtgttcgat acgtcagacg gttataagta ttttgcgccc gccaacaccg     2460

tcaacgataa tatctacggt caagctgttg aatatagtgg tttggtccgt gtcggtgaag     2520

acgtgtacta tttcggcgag acgtacacaa ttgagacggg ctggatctat gatatggaga     2580

acgagagtga caagtactat ttcaatcctg agaccaagaa agcatgcaag gggatcaacc     2640

tgatcgatga catcaagtac tatttcgacg agaagggcat tatgcgcacg gggcttatct     2700

catttgagaa taacaattat tacttcaatg agaacgggga aatgcagttt gggtacatca     2760

atattgagga caagatgttc tattttggcg aggatggcgt catgcagatc ggggtgttca     2820

acaccccaga tggtttcaag tatttcgcgc atcagaatac gctggatgag aacttcgagg     2880

gcgaatcaat caactatacc gggtggctgg acctcgatga gaagcgctac tatttcacgg     2940

acgaatacat tgcggccacc ggctcagtca tcattgatgg cgaggaatac tatttcgacc     3000

ctgatacggc gcagctggtg atcagtgagt aatgacctag ctgataacct agcccgccta     3060

atgagcgggc ttttttttct cggcctaggt ttcacctgtt ctattaggtg ttacatgctg     3120

ttcatctgtt acattgtcga tctgttcatg gtgaacagct ttaaatgcac caaaaactcg     3180

taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt     3240

tccctttgat atctaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc     3300

actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct     3360

ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatgcttac     3420

tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag     3480

catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta atggttgttg     3540

gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt     3600

gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca     3660

ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt     3720

taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa     3780

ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact     3840

cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta     3900

tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact aattctaatt     3960

tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag     4020

gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat     4080

aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg     4140

tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa     4200

ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga     4260

aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat     4320

tgagatgggc tagtcaatga taattactag ctagtccttt tcctttgagt tgtgggtatc     4380

tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat     4440

tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata     4500

aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt     4560

tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac     4620

agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat     4680

attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt     4740

cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg     4800

gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg     4860

ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc     4920

ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta     4980

atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg tcaaccggat     5040

ctaaaacact aggcccaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct     5100

tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc     5160

gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca     5220

ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt     5280

gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg     5340

cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc     5400

aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc     5460

ttctctcatc cgccaaaaca gccaagctgg atctaaaaca ctagctctag ctattgtttt     5520

aatgacaaat cagaacggaa tgtcatcatc aaagtccatc ggcggctcgt tagacggcgc     5580

tgccggagcg gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccaccctg     5640

cggctgctga ggctgacccc aaccgccctg cggctgacca ccaccgatat tgccacctgc     5700

cggagcgcca ccaccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac     5760

gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc catttacggg tacgcagctg     5820

accttcgata taaacctgag aacctttacg cagatattcg ctcgccactt ctgccagttt     5880

gccgaacagc acaacgcggt gccattcagt ctgttctttc atctcgccgg tcgctttatc     5940

acgccaggat tcggaagtag ccagcgtaat gttggcaact gcgccaccat ttggcatgta     6000

gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct     6060

ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac     6120

cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag     6180

acattgttac acaacactcc ggataatgca ttccaatact gtatattcat tcaggtcaat     6240

catatgaagg gcgaattctg cagatatcca tcacactggc ggccgccagt gtgatggata     6300

tctgcagaat tcgcccttga gaagttccta ttctatatat agtataggaa cttctctaga     6360

acttttgtta cccgccaaac aaaacccaaa aacaacccat acccaaccca ataaaacacc     6420

aaaacaagac aaataatcat tgattgatgg ttgaaatggg gtaaacttga caaacaaacc     6480

cacttaaaac ccaaaacata cccaaacaca caccaaaaaa acaccataag gagttttata     6540

aatgttggta ttcattgatg acggttcaac aaacatcaaa ctacagtggc aggaaagcga     6600

cggaacaatt aaacagcaca ttagcccgaa cagcttcaaa cgcgagtggg cagtctcttt     6660

tggtgataaa aaggtcttta actacacact gaacggcgaa cagtattcat ttgatccaat     6720

cagcccggat gctgtagtca caaccaatat cgcatggcaa tacagcgacg ttaatgtcgt     6780

tgcagtgcat cacgccttac tgaccagtgg tctgccggta agcgaagtgg atattgtttg     6840

cacacttcct ctgacagagt attacgacag aaataaccaa cccaatacgg aaaatattga     6900

gcgtaagaaa gcaaacttcc ggaaaaaaat tacattaaat ggcggggata cattcacaat     6960

aaaagatgta aaagtcatgc ctgaatctat accggcaggt tatgaagttc tacaagaact     7020

ggatgagtta gattctttat taattataga tctcgggggc accacattag atatttctca     7080

ggtaatgggg aaattatcgg ggatcagtaa aatatacgga gactcatctc ttggtgtctc     7140

tctggttaca tctgcagtaa aagatgccct ttctcttgcg agaacaaaag gaagtagcta     7200

tcttgctgac gatataatca ttcacagaaa agataataac tatctgaagc aacgaattaa     7260

tgatgagaac aaaatatcaa tagtcaccga agcaatgaat gaagcacttc gtaaacttga     7320

gcaacgtgta ttaaatacgc tcaatgaatt ttctggttat actcatgtta tggttatagg     7380

cggtggcgca gaattaatat gcgatgcagt aaaaaaacac acacagattc gtgatgaacg     7440

ttttttcaaa accaataact ctcaatatga tttagttaac ggtatgtatc tcataggtaa     7500

ttaatgatgg acaagcgcag aaccattgcc ttcaaactaa atccagatgt aaatcaaaca     7560

gataaaattg tttgtgatac actggacagt atcccgcaag gggaacgaag ccgccttaac     7620

cgggccgcac tgacggcagg tctggcctta tacagacaag atccccggac ccctttcctt     7680

ttatgtgagc tgctgacgaa agaaaccaca ttttcagata tcgtgaatat attgagatcg     7740

ctatttccaa aagagatggc cgattttaat tcttcaatag tcactcaatc ctcttcacaa     7800

caagagcaaa aaagtgatga agagaccaaa aaaaatgcga tgaagctaat aaattaattc     7860

aattattatt gagttccctt tatccactat caggctggat aaagggaact caatcaagtt     7920

attttcttac cagtcattac ataatcgtta ttatgaaata atcgtttgca ctgtctctgt     7980

tattcaggca atttcaataa aggcacttgc tcacgctctg tcattttctg aaactcttca     8040

tgctg                                                                 8045


<210>  37
<211>  838
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of ClyA-CBD/B

<400>  37

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Gly Ala Ala Ala Gly Ile Val Ala Gly Pro Phe Gly Leu 
            180                 185                 190         


Ile Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Ala 
    290                 295                 300                 


Ser Gly Leu Ile Tyr Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro 
305                 310                 315                 320 


Val Asn Asn Leu Ile Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr 
                325                 330                 335     


Tyr Phe Asn Pro Ile Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile 
            340                 345                 350         


Ile Asp Asp Lys Asn Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr 
        355                 360                 365             


Gly Val Phe Ser Thr Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn 
    370                 375                 380                 


Thr Leu Asp Glu Asn Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys 
385                 390                 395                 400 


Leu Ile Ile Asp Glu Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly 
                405                 410                 415     


Ala Val Glu Trp Lys Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro 
            420                 425                 430         


Glu Thr Gly Lys Ala Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys 
        435                 440                 445             


Tyr Tyr Phe Asn Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile 
    450                 455                 460                 


Asn Asp Asn Lys His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly 
465                 470                 475                 480 


Tyr Thr Glu Ile Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu 
                485                 490                 495     


Met Gln Ile Gly Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala 
            500                 505                 510         


His His Asn Glu Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr 
        515                 520                 525             


Ser Gly Ile Leu Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser 
    530                 535                 540                 


Phe Thr Ala Val Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr 
545                 550                 555                 560 


Tyr Phe Asp Glu Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile 
                565                 570                 575     


Asn Asp Gly Gln Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly 
            580                 585                 590         


Phe Val Thr Ile Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile 
        595                 600                 605             


Ile Glu Ser Gly Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp 
    610                 615                 620                 


Asp Asn Gly Ile Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr 
625                 630                 635                 640 


Lys Tyr Phe Ala Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln 
                645                 650                 655     


Ala Val Glu Tyr Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr 
            660                 665                 670         


Phe Gly Glu Thr Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu 
        675                 680                 685             


Asn Glu Ser Asp Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys 
    690                 695                 700                 


Lys Gly Ile Asn Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys 
705                 710                 715                 720 


Gly Ile Met Arg Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr 
                725                 730                 735     


Phe Asn Glu Asn Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp 
            740                 745                 750         


Lys Met Phe Tyr Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe 
        755                 760                 765             


Asn Thr Pro Asp Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp 
    770                 775                 780                 


Glu Asn Phe Glu Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu 
785                 790                 795                 800 


Asp Glu Lys Arg Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly 
                805                 810                 815     


Ser Val Ile Ile Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala 
            820                 825                 830         


Gln Leu Val Ile Ser Glu 
        835             


<210>  38
<211>  305
<212>  PRT
<213>  Salmonella typhi

<400>  38

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Gly Ala Ala Ala Gly Ile Val Ala Gly Pro Phe Gly Leu 
            180                 185                 190         


Ile Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Ala 
    290                 295                 300                 


Ser 
305 


<210>  39
<211>  921
<212>  DNA
<213>  Salmonella typhi

<400>  39
atgactagta tttttgcaga acaaactgta gaggtagtta aaagcgcgat cgaaaccgca       60

gatggggcat tagatcttta taacaaatac ctcgaccagg tcatcccctg gaagaccttt      120

gatgaaacca taaaagagtt aagccgtttt aaacaggagt actcgcagga agcttctgtt      180

ttagttggtg atattaaagt tttgcttatg gacagccagg acaagtattt tgaagcgaca      240

caaactgttt atgaatggtg tggtgtcgtg acgcaattac tctcagcgta tattttacta      300

tttgatgaat ataatgagaa aaaagcatca gcccagaaag acattctcat taggatatta      360

gatgatggtg tcaagaaact gaatgaagcg caaaaatctc tcctgacaag ttcacaaagt      420

ttcaacaacg cttccggaaa actgctggca ttagatagcc agttaactaa tgatttttcg      480

gaaaaaagta gttatttcca gtcacaggtg gatagaattc gtaaggaagc ttatgccggt      540

gctgcagccg gcatagtcgc cggtccgttt ggattaatta tttcctattc tattgctgcg      600

ggcgtgattg aagggaaatt gattccagaa ttgaataaca ggctaaaaac agtgcaaaat      660

ttctttacta gcttatcagc tacagtgaaa caagcgaata aagatatcga tgcggcaaaa      720

ttgaaattag ccactgaaat agcagcaatt ggggagataa aaacggaaac cgaaacaacc      780

agattctacg ttgattatga tgatttaatg ctttctttat taaaaggagc tgcaaagaaa      840

atgattaaca cctgtaatga ataccaacaa cgtcatggta agaagacgct tttcgaggtt      900

cctgacgtcg ctagctgata a                                                921


<210>  40
<211>  909
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized codon-optimized S. Typhi clyA 
       polynucleotide sequence

<400>  40
atgactagta tttttgcgga acagaccgtg gaagtggtta aaagcgcgat cgaaaccgcg       60

gatggcgcgt tagatcttta taacaaatat ctggatcagg tgattccgtg gaaaaccttt      120

gatgaaacca ttaaagaact gagccgtttt aaacaggaat atagccagga agcgagcgtg      180

ctggtgggcg atattaaagt gctgctgatg gatagccagg ataaatattt tgaagcgacc      240

cagaccgtgt atgaatggtg cggcgtggtt acccagctgc tgagcgcgta tatcctgctg      300

tttgatgaat ataacgaaaa gaaagcgagc gctcagaaag atattctgat tcgtattctg      360

gatgacggcg tgaaaaaact gaacgaagcg cagaaaagcc tgctgaccag cagccagagc      420

tttaacaatg cgtccggaaa actgctggcg ctggatagcc agctgaccaa cgattttagc      480

gaaaaaagca gctattttca gagccaggtg gatagaattc gtaaagaagc ctatgccggc      540

gctgcagccg gcattgtggc tggtccgttt ggcctgatta tcagctatag cattgccgcg      600

ggcgttattg aaggcaaact gattccggaa ctgaataacc gtctgaaaac cgttcagaat      660

ttctttacaa gcttaagcgc gaccgtgaaa caggcgaaca aagatatcga tgcggcaaaa      720

ctgaaactgg cgaccgaaat tgcggctatt ggcgaaatta aaaccgaaac cgaaaccacc      780

cgtttttatg tggattatga tgacctgatg ctgagcctgc tgaaaggcgc ggcaaagaaa      840

atgattaaca cctgcaacga atatcagcag cgtcatggca agaaaaccct gtttgaagtg      900

ccggatgtg                                                              909


<210>  41
<211>  1102
<212>  DNA
<213>  Salmonella typhi

<400>  41
ggaggtaata ggtaagaata ctttataaaa caggtactta attgcaattt atatatttaa       60

agaggcaaat gattatgacc ggaatatttg cagaacaaac tgtagaggta gttaaaagcg      120

cgatcgaaac cgcagatggg gcattagatc tttataacaa atacctcgac caggtcatcc      180

cctggaagac ctttgatgaa accataaaag agttaagccg ttttaaacag gagtactcgc      240

aggaagcttc tgttttagtt ggtgatatta aagttttgct tatggacagc caggacaagt      300

attttgaagc gacacaaact gtttatgaat ggtgtggtgt cgtgacgcaa ttactctcag      360

cgtatatttt actatttgat gaatataatg agaaaaaagc atcagcccag aaagacattc      420

tcattaggat attagatgat ggtgtcaaga aactgaatga agcgcaaaaa tctctcctga      480

caagttcaca aagtttcaac aacgcttccg gaaaactgct ggcattagat agccagttaa      540

ctaatgattt ttcggaaaaa agtagttatt tccagtcaca ggtggataga attcgtaagg      600

aagcttatgc cggtgctgca gccggcatag tcgccggtcc gtttggatta attatttcct      660

attctattgc tgcgggcgtg attgaaggga aattgattcc agaattgaat aacaggctaa      720

aaacagtgca aaatttcttt actagcttat cagctacagt gaaacaagcg aataaagata      780

tcgatgcggc aaaattgaaa ttagccactg aaatagcagc aattggggag ataaaaacgg      840

aaaccgaaac aaccagattc tacgttgatt atgatgattt aatgctttct ttattaaaag      900

gagctgcaaa gaaaatgatt aacacctgta atgaatacca acaaagacac ggtaagaaga      960

cgcttttcga ggttcctgac gtctgataca ttttcattcg atctgtgtac ttttaacgcc     1020

cgatagcgta aagaaaatga gagacggaga aaaagcgata ttcaacagcc cgataaacaa     1080

gagtcgttac cgggctgacg ag                                              1102


<210>  42
<211>  1102
<212>  DNA
<213>  Salmonella paratyphi

<400>  42
ggaggcaata ggtaggaata agttataaaa caatagctta attgcaattt atatatttaa       60

agaggcaaat gattatgact ggaatatttg cagaacaaac tgtagaggta gttaaaagcg      120

cgatcgaaac cgcagatggg gcattagatt tttataacaa atacctcgac caggttatcc      180

cctggaagac ctttgatgaa accataaaag agttaagccg ttttaaacag gagtactcgc      240

aggaagcttc tgttttagtt ggtgatatta aagttttgct tatggacagc caggataagt      300

attttgaagc gacacaaact gtttatgaat ggtgtggtgt cgtgacgcaa ttactctcag      360

cgtatatttt actatttgat gaatataatg agaaaaaagc atcagcgcag aaagacattc      420

tcatcaggat attagatgat ggcgtcaata aactgaatga agcgcaaaaa tctctcctgg      480

gaagttcaca aagtttcaac aacgcttcag gaaaactgct ggcattagat agccagttaa      540

ctaatgattt ctcggaaaaa agtagttatt tccagtcaca ggtggataga attcgtaagg      600

aagcttatgc cggtgctgca gcaggcatag tcgccggtcc gtttggatta attatttcct      660

attctattgc tgcgggcgtg attgaaggga aattgattcc agaattgaat gacaggctaa      720

aagcagtgca aaatttcttt actagcttat cagtcacagt gaaacaagcg aataaagata      780

tcgatgcggc aaaattgaaa ttagccactg aaatagcagc aattggggag ataaaaacgg      840

aaaccgaaac aaccagattc tacgttgatt atgatgattt aatgctttct ttactaaaag      900

gagctgcaaa gaaaatgatt aacacctgta atgaatacca acaaaggcac ggtaagaaga      960

cgcttctcga ggttcctgac atctgataca ttttcattcg ctctgtttac ttttaacgcc     1020

cgatagcgtg aagaaaatga gagacggaga aaaagcgata ttcaacagcc cgataaacaa     1080

gagtcgttac cgggctggcg ag                                              1102


<210>  43
<211>  904
<212>  DNA
<213>  Shigella flexneri

<400>  43
atgactgaaa tcgttgcaga taaaacggta gaagtagtta aaaacgcaat cgaaaccgca       60

gatggagcat tagatcttta taataaatat ctcgatcagg tcatcccctg gcagaccttt      120

gatgaaacca taaaagagtt aagtcgcttt aaacaggagt attcacaggc agcctccgtt      180

ttagtcggcg atattaaaac cttacttatg gatagccagg ataagtattt tgaagcaacc      240

caaacagtgt atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta tattttgcta      300

tttgatgagt acaatgagaa gaaagcatcc gcccctcatt aaggtactgg atgacggcat      360

cacgaagctg aatgaagcgc aaaattccct gctggtaagc tcacaaagtt tcaacaacgc      420

ttccgggaaa ctgctggcgt tagatagcca gttaaccaat gatttttcag aaaaaagcag      480

ctatttccag tcacaggtag ataaaatcag gaaggaagcg tatgccggtg ccgcagccgg      540

tgtcgtcgcc ggtccatttg gtttaatcat ttcctattct attgctgcgg gcgtagttga      600

agggaaactg attccagaat tgaagaacaa gttaaaatct gtgcagagtt tctttaccac      660

cctgtctaac acggttaaac aagcgaataa agatatcgat gccgccaaat tgaaattaac      720

caccgaaata gccgccatcg gggagataaa aacggaaact gaaaccacca gattctatgt      780

tgattatgat gatttaatgc tttctttgct aaaagcagcg gccaaaaaaa tgattaacac      840

ctgtaatgag tatcagaaaa gacacggtaa aaagacactc tttgaggtac ctgaagtctg      900

ataa                                                                   904


<210>  44
<211>  1080
<212>  DNA
<213>  Escherichia coli

<400>  44
agaaataaag acattgacgc atcccgcccg gctaactatg aattagatga agtaaaattt       60

attaatagtt gtaaaacagg agtttcatta caatttatat atttaaagag gcgaatgatt      120

atgactgaaa tcgttgcaga taaaacggta gaagtagtta aaaacgcaat cgaaaccgca      180

gatggagcat tagatcttta taataaatat ctcgatcagg tcatcccctg gcagaccttt      240

gatgaaacca taaaagagtt aagtcgcttt aaacaggagt attcacaggc agcctccgtt      300

ttagtcggcg atattaaaac cttacttatg gatagccagg ataagtattt tgaagcaacc      360

caaacagtgt atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta tattttgcta      420

tttgatgagt acaatgagaa gaaagcatcc gcccagaaag acattctcat taaggtactg      480

gatgacggca tcacgaagct gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt      540

ttcaacaacg cttccgggaa actgctggcg ttagatagcc agttaaccaa tgatttttca      600

gaaaaaagca gctatttcca gtcacaggta gataaaatca ggaaggaagc atatgccggt      660

gccgcagccg gtgtcgtcgc cggtccattt ggattaatca tttcctattc tattgctgcg      720

ggcgtagttg aaggaaaact gattccagaa ttgaagaaca agttaaaatc tgtgcagaat      780

ttctttacca ccctgtctaa cacggttaaa caagcgaata aagatatcga tgccgccaaa      840

ttgaaattaa ccaccgaaat agccgccatc ggtgagataa aaacggaaac tgaaacaacc      900

agattctacg ttgattatga tgatttaatg ctttctttgc taaaagaagc ggccaaaaaa      960

atgattaaca cctgtaatga gtatcagaaa agacacggta aaaagacact ctttgaggta     1020

cctgaagtct gataagcgat tattctctcc atgtactcaa ggtataaggt ttatcacatt     1080


