
                                SEQUENCE LISTING

<110> GENOMATICA, Inc.
      Osterhout, Robin E.
      Burgard, Anthony P.

<120> MICROORGANISMS AND METHODS FOR PRODUCTION OF SPECIFIC 
      LENGTH FATTY ALCOHOLS AND RELATED COMPOUNDS

<130> 12956-210-228

<140> PCT/US2013/064827
<141> 2013-10-14

<150> 61/714,144          
<151> 2012-10-15  

<160> 23

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> lacZalpha-RI primer

<400> 1
gacgaattcg ctagcaagag gagaagtcga catgtccaat tcactggccg tcgttttac  59

<210> 2
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> lacZalpha 3prime-BB primer

<400> 2
gaccctagga agctttctag agtcgaccta tgcggcatca gagcaga               47

<210> 3
<211> 474
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated LpdA from E. coli K-12 MG1655

<400> 3
Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro
 1               5                  10                  15      
Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr
            20                  25                  30          
Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val
        35                  40                  45              
Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu
    50                  55                  60                  
Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys
65                  70                  75                  80  
Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln
                85                  90                  95      
Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val
            100                 105                 110         
Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu
        115                 120                 125             
Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala
    130                 135                 140                 
Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro
145                 150                 155                 160 
Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu
                165                 170                 175     
Arg Leu Leu Val Met Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Thr
            180                 185                 190         
Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Val Arg Lys His
        195                 200                 205             
Gln Val Ile Arg Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys
    210                 215                 220                 
Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala
225                 230                 235                 240 
Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys
                245                 250                 255     
Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly
            260                 265                 270         
Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu
        275                 280                 285             
Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn
    290                 295                 300                 
Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu
305                 310                 315                 320 
Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala
                325                 330                 335     
Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr
            340                 345                 350         
Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys
        355                 360                 365             
Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser
    370                 375                 380                 
Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile
385                 390                 395                 400 
Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr
                405                 410                 415     
Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly
            420                 425                 430         
Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu
        435                 440                 445             
His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr
    450                 455                 460                 
Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys
465                 470                 


<210> 4
<211> 474
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated LpdA from E. coli K-12 MG1655

<400> 4
Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro
 1               5                  10                  15      
Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr
            20                  25                  30          
Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val
        35                  40                  45              
Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu
    50                  55                  60                  
Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys
65                  70                  75                  80  
Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln
                85                  90                  95      
Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val
            100                 105                 110         
Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu
        115                 120                 125             
Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala
    130                 135                 140                 
Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro
145                 150                 155                 160 
Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu
                165                 170                 175     
Arg Leu Leu Val Met Gly Gly Gly Ile Ile Ala Leu Glu Met Ala Thr
            180                 185                 190         
Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Val Arg Lys His
        195                 200                 205             
Gln Val Ile Arg Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys
    210                 215                 220                 
Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala
225                 230                 235                 240 
Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys
                245                 250                 255     
Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly
            260                 265                 270         
Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu
        275                 280                 285             
Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn
    290                 295                 300                 
Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu
305                 310                 315                 320 
Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala
                325                 330                 335     
Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr
            340                 345                 350         
Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys
        355                 360                 365             
Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser
    370                 375                 380                 
Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile
385                 390                 395                 400 
Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr
                405                 410                 415     
Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly
            420                 425                 430         
Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu
        435                 440                 445             
His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr
    450                 455                 460                 
Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys
465                 470                 


<210> 5
<211> 364
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutant Candida bodinii enzyme 

<400> 5
Met Lys Ile Val Leu Val Leu Tyr Asp Ala Gly Lys His Ala Ala Asp
 1               5                  10                  15      
Glu Glu Lys Leu Tyr Gly Cys Thr Glu Asn Lys Leu Gly Ile Ala Asn
            20                  25                  30          
Trp Leu Lys Asp Gln Gly His Glu Leu Ile Thr Thr Ser Asp Lys Glu
        35                  40                  45              
Gly Glu Thr Ser Glu Leu Asp Lys His Ile Pro Asp Ala Asp Ile Ile
    50                  55                  60                  
Ile Thr Thr Pro Phe His Pro Ala Tyr Ile Thr Lys Glu Arg Leu Asp
65                  70                  75                  80  
Lys Ala Lys Asn Leu Lys Leu Val Val Val Ala Gly Val Gly Ser Asp
                85                  90                  95      
His Ile Asp Leu Asp Tyr Ile Asn Gln Thr Gly Lys Lys Ile Ser Val
            100                 105                 110         
Leu Glu Val Thr Gly Ser Asn Val Val Ser Val Ala Glu His Val Val
        115                 120                 125             
Met Thr Met Leu Val Leu Val Arg Asn Phe Val Pro Ala His Glu Gln
    130                 135                 140                 
Ile Ile Asn His Asp Trp Glu Val Ala Ala Ile Ala Lys Asp Ala Tyr
145                 150                 155                 160 
Asp Ile Glu Gly Lys Thr Ile Ala Thr Ile Gly Ala Gly Arg Ile Gly
                165                 170                 175     
Tyr Arg Val Leu Glu Arg Leu Leu Pro Phe Asn Pro Lys Glu Leu Leu
            180                 185                 190         
Tyr Tyr Gln Arg Gln Ala Leu Pro Lys Glu Ala Glu Glu Lys Val Gly
        195                 200                 205             
Ala Arg Arg Val Glu Asn Ile Glu Glu Leu Val Ala Gln Ala Asp Ile
    210                 215                 220                 
Val Thr Val Asn Ala Pro Leu His Ala Gly Thr Lys Gly Leu Ile Asn
225                 230                 235                 240 
Lys Glu Leu Leu Ser Lys Phe Lys Lys Gly Ala Trp Leu Val Asn Thr
                245                 250                 255     
Ala Arg Gly Ala Ile Cys Val Ala Glu Asp Val Ala Ala Ala Leu Glu
            260                 265                 270         
Ser Gly Gln Leu Arg Gly Tyr Gly Gly Asp Val Trp Phe Pro Gln Pro
        275                 280                 285             
Ala Pro Lys Asp His Pro Trp Arg Asp Met Arg Asn Lys Tyr Gly Ala
    290                 295                 300                 
Gly Asn Ala Met Thr Pro His Tyr Ser Gly Thr Thr Leu Asp Ala Gln
305                 310                 315                 320 
Thr Arg Tyr Ala Glu Gly Thr Lys Asn Ile Leu Glu Ser Phe Phe Thr
                325                 330                 335     
Gly Lys Phe Asp Tyr Arg Pro Gln Asp Ile Ile Leu Leu Asn Gly Glu
            340                 345                 350         
Tyr Val Thr Lys Ala Tyr Gly Lys His Asp Lys Lys
        355                 360                 


<210> 6
<211> 364
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutant Candida bodinii enzyme 

<400> 6
Met Lys Ile Val Leu Val Leu Tyr Asp Ala Gly Lys His Ala Ala Asp
 1               5                  10                  15      
Glu Glu Lys Leu Tyr Gly Cys Thr Glu Asn Lys Leu Gly Ile Ala Asn
            20                  25                  30          
Trp Leu Lys Asp Gln Gly His Glu Leu Ile Thr Thr Ser Asp Lys Glu
        35                  40                  45              
Gly Glu Thr Ser Glu Leu Asp Lys His Ile Pro Asp Ala Asp Ile Ile
    50                  55                  60                  
Ile Thr Thr Pro Phe His Pro Ala Tyr Ile Thr Lys Glu Arg Leu Asp
65                  70                  75                  80  
Lys Ala Lys Asn Leu Lys Leu Val Val Val Ala Gly Val Gly Ser Asp
                85                  90                  95      
His Ile Asp Leu Asp Tyr Ile Asn Gln Thr Gly Lys Lys Ile Ser Val
            100                 105                 110         
Leu Glu Val Thr Gly Ser Asn Val Val Ser Val Ala Glu His Val Val
        115                 120                 125             
Met Thr Met Leu Val Leu Val Arg Asn Phe Val Pro Ala His Glu Gln
    130                 135                 140                 
Ile Ile Asn His Asp Trp Glu Val Ala Ala Ile Ala Lys Asp Ala Tyr
145                 150                 155                 160 
Asp Ile Glu Gly Lys Thr Ile Ala Thr Ile Gly Ala Gly Arg Ile Gly
                165                 170                 175     
Tyr Arg Val Leu Glu Arg Leu Leu Pro Phe Asn Pro Lys Glu Leu Leu
            180                 185                 190         
Tyr Tyr Ser Pro Gln Ala Leu Pro Lys Glu Ala Glu Glu Lys Val Gly
        195                 200                 205             
Ala Arg Arg Val Glu Asn Ile Glu Glu Leu Val Ala Gln Ala Asp Ile
    210                 215                 220                 
Val Thr Val Asn Ala Pro Leu His Ala Gly Thr Lys Gly Leu Ile Asn
225                 230                 235                 240 
Lys Glu Leu Leu Ser Lys Phe Lys Lys Gly Ala Trp Leu Val Asn Thr
                245                 250                 255     
Ala Arg Gly Ala Ile Cys Val Ala Glu Asp Val Ala Ala Ala Leu Glu
            260                 265                 270         
Ser Gly Gln Leu Arg Gly Tyr Gly Gly Asp Val Trp Phe Pro Gln Pro
        275                 280                 285             
Ala Pro Lys Asp His Pro Trp Arg Asp Met Arg Asn Lys Tyr Gly Ala
    290                 295                 300                 
Gly Asn Ala Met Thr Pro His Tyr Ser Gly Thr Thr Leu Asp Ala Gln
305                 310                 315                 320 
Thr Arg Tyr Ala Glu Gly Thr Lys Asn Ile Leu Glu Ser Phe Phe Thr
                325                 330                 335     
Gly Lys Phe Asp Tyr Arg Pro Gln Asp Ile Ile Leu Leu Asn Gly Glu
            340                 345                 350         
Tyr Val Thr Lys Ala Tyr Gly Lys His Asp Lys Lys
        355                 360                 


<210> 7
<211> 376
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutant Saccharomyces cerevisiae 

<400> 7
Met Ser Lys Gly Lys Val Leu Leu Val Leu Tyr Glu Gly Gly Lys His
 1               5                  10                  15      
Ala Glu Glu Gln Glu Lys Leu Leu Gly Cys Ile Glu Asn Glu Leu Gly
            20                  25                  30          
Ile Arg Asn Phe Ile Glu Glu Gln Gly Tyr Glu Leu Val Thr Thr Ile
        35                  40                  45              
Asp Lys Asp Pro Glu Pro Thr Ser Thr Val Asp Arg Glu Leu Lys Asp
    50                  55                  60                  
Ala Glu Ile Val Ile Thr Thr Pro Phe Phe Pro Ala Tyr Ile Ser Arg
65                  70                  75                  80  
Asn Arg Ile Ala Glu Ala Pro Asn Leu Lys Leu Cys Val Thr Ala Gly
                85                  90                  95      
Val Gly Ser Asp His Val Asp Leu Glu Ala Ala Asn Glu Arg Lys Ile
            100                 105                 110         
Thr Val Thr Glu Val Thr Gly Ser Asn Val Val Ser Val Ala Glu His
        115                 120                 125             
Val Met Ala Thr Ile Leu Val Leu Ile Arg Asn Tyr Asn Gly Gly His
    130                 135                 140                 
Gln Gln Ala Ile Asn Gly Glu Trp Asp Ile Ala Gly Val Ala Lys Asn
145                 150                 155                 160 
Glu Tyr Asp Leu Glu Asp Lys Ile Ile Ser Thr Val Gly Ala Gly Arg
                165                 170                 175     
Ile Gly Tyr Arg Val Leu Glu Arg Leu Val Ala Phe Asn Pro Lys Lys
            180                 185                 190         
Leu Leu Tyr Tyr Ala Arg Gln Glu Leu Pro Ala Glu Ala Ile Asn Arg
        195                 200                 205             
Leu Asn Glu Ala Ser Lys Leu Phe Asn Gly Arg Gly Asp Ile Val Gln
    210                 215                 220                 
Arg Val Glu Lys Leu Glu Asp Met Val Ala Gln Ser Asp Val Val Thr
225                 230                 235                 240 
Ile Asn Cys Pro Leu His Lys Asp Ser Arg Gly Leu Phe Asn Lys Lys
                245                 250                 255     
Leu Ile Ser His Met Lys Asp Gly Ala Tyr Leu Val Asn Thr Ala Arg
            260                 265                 270         
Gly Ala Ile Cys Val Ala Glu Asp Val Ala Glu Ala Val Lys Ser Gly
        275                 280                 285             
Lys Leu Ala Gly Tyr Gly Gly Asp Val Trp Asp Lys Gln Pro Ala Pro
    290                 295                 300                 
Lys Asp His Pro Trp Arg Thr Met Asp Asn Lys Asp His Val Gly Asn
305                 310                 315                 320 
Ala Met Thr Val His Ile Ser Gly Thr Ser Leu Asp Ala Gln Lys Arg
                325                 330                 335     
Tyr Ala Gln Gly Val Lys Asn Ile Leu Asn Ser Tyr Phe Ser Lys Lys
            340                 345                 350         
Phe Asp Tyr Arg Pro Gln Asp Ile Ile Val Gln Asn Gly Ser Tyr Ala
        355                 360                 365             
Thr Arg Ala Tyr Gly Gln Lys Lys
    370                 375     


<210> 8
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Thl 1502 gene

<400> 8
tctaatctaa gttttctaga actagtaaag atgagagatg tagtaatagt aagtgctgta 60


<210> 9
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Thl 1502 gene

<400> 9
gatatcgaat tcctgcagcc cgggggatcc ttagtctctt tcaactacga gagctgtt   58

<210> 10
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Thl 1491 gene

<400> 10
tctaatctaa gttttctaga actagtaaag atgaaaaatt gtgtcatcgt cagtg      55

<210> 11
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Thl 1491 gene

<400> 11
gatatcgaat tcctgcagcc cgggggatcc ttaattcaac cgttcaatca ccatcgcaat 60


<210> 12
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Thl 560 gene

<400> 12
aatctaagtt ttctagaact agtaaagatg aaagaagttg taatagctag tgcagtaa   58

<210> 13
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Thl 560 gene

<400> 13
tatcgaattc ctgcagcccg ggggatcctt aatggtgatg gtgatgatgg cacttttcta 60


<210> 14
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Thl 1512 gene

<400> 14
tctaatctaa gttttctaga actagtaaag atgagcaccc cgtccatcgt ca         52

<210> 15
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Thl 1512 gene

<400> 15
gatatcgaat tcctgcagcc cgggggatcc ctaaaggctc tcgatgcaca tcgcc      55

<210> 16
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Thl 1501 gene

<400> 16
taagctagca agaggagaag tcgacatgac tgacgttgtc atcgtatccg c          51

<210> 17
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Thl 1501 gene

<400> 17
gcctctagga agctttctag attattattt gcgctcgact gccagc                46

<210> 18
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Hbd 1495 gene

<400> 18
aagcatacaa tcaactatct catatacaat gaaaaagatt tttgtacttg gagca      55

<210> 19
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Hbd 1495 gene

<400> 19
aaaaatcata aatcataaga aattcgctta tttagagtaa tcatagaatc cttttcctga 60


<210> 20
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Ald 707 gene

<400> 20
aatctaagtt ttctagaact agtaaagatg aacacagaaa acattgaaca agccat     56

<210> 21
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Ald 707 gene

<400> 21
tatcgaattc ctgcagcccg ggggatccct aagcctccca agtccgtaat gagaaccctt 60


<210> 22
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer used to amplify Adh 28 gene

<400> 22
ccaagcatac aatcaactat ctcatataca atggagaatt ttagatttaa tgcatataca 60


<210> 23
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer used to amplify Adh 28 gene

<400> 23
aataaaaatc ataaatcata agaaattcgc ttaaagggac atttctaaaa ttttatatac 60

