                         SEQUENCE LISTING

<110>  ExxonMobil Research and Engineering Company
       Roy, Gena
       Schneider, Jane C.
       Moellering, Eric
       Holtzapple, Erik
       Lieberman, Soyan
 
<120>  DGAT GENES AND METHODS OF USE FOR TRIGLYCERIDE PRODUCTION IN RECOMBINANT MICROORGANISMS

<130>  2012EM274-WO / 16244-000014/WO/POA

<160>  87    

<170>  PatentIn version 3.5

<210>  1
<211>  2251
<212>  DNA
<213>  Mus musculus

<400>  1
ccgcgcttcg ctggctttct gctcatctag ggtggcagcg gctacctacc tcagctctcg       60

ccctgctgcc gccacggcct gggcgctgtc cctcagctcc cggagctcag cgcgaagccc      120

tggccccggc ggccggggca tgggtcaggg gcgcggcgtg aggcggcttt ctgcacggcc      180

gtgacgtgca ttggcttcag catgaagacc ctcatcgccg cctactccgg ggtcctgcgg      240

ggtgagcgtc gggcggaagc tgcccgcagc gaaaacaaga ataaaggatc tgccctgtca      300

cgcgaggggt ctgggcgatg gggcactggc tccagcatcc tctcagccct ccaagacatc      360

ttctctgtca cctggctcaa cagatctaag gtggaaaaac agctgcaggt catctcagta      420

ctacaatggg tcctatcctt cctggtgcta ggagtggcct gcagtgtcat cctcatgtac      480

accttctgca cagactgctg gctgatagct gtgctctact tcacctggct ggcatttgac      540

tggaacacgc ccaagaaagg tggcaggaga tcgcagtggg tgcgaaactg ggccgtgtgg      600

cgctacttcc gagactactt tcccatccag ctggtgaaga cacacaacct gctgaccacc      660

aggaactata tctttggata ccacccccat ggcatcatgg gcctgggtgc cttctgtaac      720

ttcagcacag aggctactga agtcagcaag aagtttcctg gcataaggcc ctatttggct      780

acgttggctg gtaacttccg gatgcctgtg cttcgcgagt acctgatgtc tggaggcatc      840

tgccctgtca accgagacac catagactac ttgctctcca agaatgggag tggcaatgct      900

atcatcatcg tggtgggagg tgcagctgag tccctgagct ccatgcctgg caagaacgca      960

gtcaccctga agaaccgcaa aggctttgtg aagctggccc tgcgccatgg agctgatctg     1020

gttcccactt attcctttgg agagaatgag gtatacaagc aggtgatctt tgaggagggt     1080

tcctggggcc gatgggtcca gaagaagttc cagaagtata ttggtttcgc cccctgcatc     1140

ttccatggcc gaggcctctt ctcctctgac acctgggggc tggtgcccta ctccaagccc     1200

atcaccaccg tcgtggggga gcccatcact gtccccaagc tggagcaccc gacccagaaa     1260

gacatcgacc tgtaccatgc catgtacatg gaggccctgg tgaagctctt tgacaatcac     1320

aagaccaaat ttggccttcc agagactgag gtgctggagg tgaactgacc cagccctcgc     1380

gtgccagctc ctgggaggga cgactgcaga tccttttcta ccgagttctt gagtgcattt     1440

tgttctgtaa atttggaagc gtcatgggtg tctgtgggtt atttaaaaga aattataatt     1500

tgttaaacca ttgcaatgtt agatgttttt taagaaggga agagtcagta ttttaagctc     1560

acttctagtg tgtcctgctc aaggtggagg ctgatattta tgggccttgg tggtttctta     1620

cccacccctt ctagcgttcc ccagacgaca gacacttggc cctggctagc tgggcaaggg     1680

cagtccttag tgactccagg gattcttgag aggcagaggc catgtcccac ccgtggctgc     1740

aggtcgggtt cctcgtacca aggggaggct gagggcacag ctggccccac ttggggaggg     1800

tagataacat ctggactgcc cggcttgggt ctctgctcct caccctagcc ctcttctcca     1860

atctgagcct accctggcct cctgtctcct ggctagggac acggctgtcc cacaggtgcc     1920

gtcttgggtt atctcgctgc tgttggctgg tttcactctg gaggttggca ccatggacac     1980

agctcagcgt tgctctggcg catatcctcc tgagccacac cccaagtctg gtgtgaggaa     2040

gggcttctct tctcttcaca gaggtgcctg gcttcctgtg cagcacactg ggtccaggac     2100

aggaggcccc ccccccaaac caagcctcac gtgtgtgcct ttatgaggcg ttgggagaaa     2160

gctaccctcc tgtgtattct gttttctcca tgagattgtt gtgccatgtc acacttttgt     2220

atattcctag actaataaat ggaaacaaga a                                    2251


<210>  2
<211>  388
<212>  PRT
<213>  Mus musculus

<400>  2

Met Lys Thr Leu Ile Ala Ala Tyr Ser Gly Val Leu Arg Gly Glu Arg 
1               5                   10                  15      


Arg Ala Glu Ala Ala Arg Ser Glu Asn Lys Asn Lys Gly Ser Ala Leu 
            20                  25                  30          


Ser Arg Glu Gly Ser Gly Arg Trp Gly Thr Gly Ser Ser Ile Leu Ser 
        35                  40                  45              


Ala Leu Gln Asp Ile Phe Ser Val Thr Trp Leu Asn Arg Ser Lys Val 
    50                  55                  60                  


Glu Lys Gln Leu Gln Val Ile Ser Val Leu Gln Trp Val Leu Ser Phe 
65                  70                  75                  80  


Leu Val Leu Gly Val Ala Cys Ser Val Ile Leu Met Tyr Thr Phe Cys 
                85                  90                  95      


Thr Asp Cys Trp Leu Ile Ala Val Leu Tyr Phe Thr Trp Leu Ala Phe 
            100                 105                 110         


Asp Trp Asn Thr Pro Lys Lys Gly Gly Arg Arg Ser Gln Trp Val Arg 
        115                 120                 125             


Asn Trp Ala Val Trp Arg Tyr Phe Arg Asp Tyr Phe Pro Ile Gln Leu 
    130                 135                 140                 


Val Lys Thr His Asn Leu Leu Thr Thr Arg Asn Tyr Ile Phe Gly Tyr 
145                 150                 155                 160 


His Pro His Gly Ile Met Gly Leu Gly Ala Phe Cys Asn Phe Ser Thr 
                165                 170                 175     


Glu Ala Thr Glu Val Ser Lys Lys Phe Pro Gly Ile Arg Pro Tyr Leu 
            180                 185                 190         


Ala Thr Leu Ala Gly Asn Phe Arg Met Pro Val Leu Arg Glu Tyr Leu 
        195                 200                 205             


Met Ser Gly Gly Ile Cys Pro Val Asn Arg Asp Thr Ile Asp Tyr Leu 
    210                 215                 220                 


Leu Ser Lys Asn Gly Ser Gly Asn Ala Ile Ile Ile Val Val Gly Gly 
225                 230                 235                 240 


Ala Ala Glu Ser Leu Ser Ser Met Pro Gly Lys Asn Ala Val Thr Leu 
                245                 250                 255     


Lys Asn Arg Lys Gly Phe Val Lys Leu Ala Leu Arg His Gly Ala Asp 
            260                 265                 270         


Leu Val Pro Thr Tyr Ser Phe Gly Glu Asn Glu Val Tyr Lys Gln Val 
        275                 280                 285             


Ile Phe Glu Glu Gly Ser Trp Gly Arg Trp Val Gln Lys Lys Phe Gln 
    290                 295                 300                 


Lys Tyr Ile Gly Phe Ala Pro Cys Ile Phe His Gly Arg Gly Leu Phe 
305                 310                 315                 320 


Ser Ser Asp Thr Trp Gly Leu Val Pro Tyr Ser Lys Pro Ile Thr Thr 
                325                 330                 335     


Val Val Gly Glu Pro Ile Thr Val Pro Lys Leu Glu His Pro Thr Gln 
            340                 345                 350         


Lys Asp Ile Asp Leu Tyr His Ala Met Tyr Met Glu Ala Leu Val Lys 
        355                 360                 365             


Leu Phe Asp Asn His Lys Thr Lys Phe Gly Leu Pro Glu Thr Glu Val 
    370                 375                 380                 


Leu Glu Val Asn 
385             


<210>  3
<211>  1056
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mus musculus MmDGAT2 truncated by 55 amino acid residues

<400>  3
gccccgactc tagaagatct ttattacaac tcgtcgtcgt tgacctccag cacttctgtt       60

tcgggaagcc cgaacttggt cttatggtta tcaaacagct tcaccaacgc ttccatatac      120

atcgcgtgat aaagatcaat gtccttttga gtgggatgct ccagtttcgg gaccgtaatt      180

ggctctccga ccacggtggt aatcggcttg ctataaggca ccagccccca cgtgtcggag      240

gaaaacaggc cacgcccgtg gaaaatgcag ggagcgaacc cgatgtactt ctggaacttt      300

ttctggaccc acctgcccca gcttccctct tcaaagatga cctgcttgta cacctcgttc      360

tcgccaaagc tgtacgtggg caccagatcg gcaccgtgcc gcagggccag cttcacaaag      420

cccttgcgat tcttgagagt aactgcattc tttcccggca tcgaggagag ggactcagca      480

gctccgccga ccacaatgat gattgcgttg ccagagccat tcttggagag aaggtagtcg      540

atggtgtcac gattaacggg acaaattccc ccggacatca ggtactctcg caacacaggc      600

atacggaagt tgcccgcgag ggtggccaag taaggacgaa tgccggggaa cttcttggac      660

acttccgtgg cttccgtcga aaagttgcag aacgcaccaa gccccatgat cccgtgcgga      720

tggtagccaa agatgtagtt ccgggtagtg agcaagttat gggttttgac caattgaata      780

gggaagtagt cgcggaagta ccgccacacg gcccaattgc gcacccactg cgaccggcga      840

ccaccctttt tgggggtgtt ccagtcgaat gcaagccagg tgaagtacaa caccgcgatg      900

agccaacaat ctgtgcagaa cgtatacatc aagatgacag agcaggccac cccgagcacg      960

agaaagctga ggacccactg aaggacagag ataacttgca attgtttctc caccttgctg     1020

cggttgagcc acgtcaccat gttggcggca agcttg                               1056


<210>  4
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mus musculus MmDGAT2 truncated by 55 amino acid residues

<400>  4

Met Val Thr Trp Leu Asn Arg Ser Lys Val Glu Lys Gln Leu Gln Val 
1               5                   10                  15      


Ile Ser Val Leu Gln Trp Val Leu Ser Phe Leu Val Leu Gly Val Ala 
            20                  25                  30          


Cys Ser Val Ile Leu Met Tyr Thr Phe Cys Thr Asp Cys Trp Leu Ile 
        35                  40                  45              


Ala Val Leu Tyr Phe Thr Trp Leu Ala Phe Asp Trp Asn Thr Pro Lys 
    50                  55                  60                  


Lys Gly Gly Arg Arg Ser Gln Trp Val Arg Asn Trp Ala Val Trp Arg 
65                  70                  75                  80  


Tyr Phe Arg Asp Tyr Phe Pro Ile Gln Leu Val Lys Thr His Asn Leu 
                85                  90                  95      


Leu Thr Thr Arg Asn Tyr Ile Phe Gly Tyr His Pro His Gly Ile Met 
            100                 105                 110         


Gly Leu Gly Ala Phe Cys Asn Phe Ser Thr Glu Ala Thr Glu Val Ser 
        115                 120                 125             


Lys Lys Phe Pro Gly Ile Arg Pro Tyr Leu Ala Thr Leu Ala Gly Asn 
    130                 135                 140                 


Phe Arg Met Pro Val Leu Arg Glu Tyr Leu Met Ser Gly Gly Ile Cys 
145                 150                 155                 160 


Pro Val Asn Arg Asp Thr Ile Asp Tyr Leu Leu Ser Lys Asn Gly Ser 
                165                 170                 175     


Gly Asn Ala Ile Ile Ile Val Val Gly Gly Ala Ala Glu Ser Leu Ser 
            180                 185                 190         


Ser Met Pro Gly Lys Asn Ala Val Thr Leu Lys Asn Arg Lys Gly Phe 
        195                 200                 205             


Val Lys Leu Ala Leu Arg His Gly Ala Asp Leu Val Pro Thr Tyr Ser 
    210                 215                 220                 


Phe Gly Glu Asn Glu Val Tyr Lys Gln Val Ile Phe Glu Glu Gly Ser 
225                 230                 235                 240 


Trp Gly Arg Trp Val Gln Lys Lys Phe Gln Lys Tyr Ile Gly Phe Ala 
                245                 250                 255     


Pro Cys Ile Phe His Gly Arg Gly Leu Phe Ser Ser Asp Thr Trp Gly 
            260                 265                 270         


Leu Val Pro Tyr Ser Lys Pro Ile Thr Thr Val Val Gly Glu Pro Ile 
        275                 280                 285             


Thr Val Pro Lys Leu Glu His Pro Thr Gln Lys Asp Ile Asp Leu Tyr 
    290                 295                 300                 


His Ala Met Tyr Met Glu Ala Leu Val Lys Leu Phe Asp Asn His Lys 
305                 310                 315                 320 


Thr Lys Phe Gly Leu Pro Glu Thr Glu Val Leu Glu Val Asn Asp Asp 
                325                 330                 335     


Glu Leu 
        


<210>  5
<211>  375
<212>  DNA
<213>  Streptoalloteichus hindustanus

<400>  5
atggccaagc tgaccagcgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc       60

gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt      120

gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac      180

aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag      240

gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag      300

ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc      360

gaggagcagg actaa                                                       375


<210>  6
<211>  124
<212>  PRT
<213>  Streptoalloteichus hindustanus

<400>  6

Met Ala Lys Leu Thr Ser Ala Val Pro Val Leu Thr Ala Arg Asp Val 
1               5                   10                  15      


Ala Gly Ala Val Glu Phe Trp Thr Asp Arg Leu Gly Phe Ser Arg Asp 
            20                  25                  30          


Phe Val Glu Asp Asp Phe Ala Gly Val Val Arg Asp Asp Val Thr Leu 
        35                  40                  45              


Phe Ile Ser Ala Val Gln Asp Gln Val Val Pro Asp Asn Thr Leu Ala 
    50                  55                  60                  


Trp Val Trp Val Arg Gly Leu Asp Glu Leu Tyr Ala Glu Trp Ser Glu 
65                  70                  75                  80  


Val Val Ser Thr Asn Phe Arg Asp Ala Ser Gly Pro Ala Met Thr Glu 
                85                  90                  95      


Ile Gly Glu Gln Pro Trp Gly Arg Glu Phe Ala Leu Arg Asp Pro Ala 
            100                 105                 110         


Gly Asn Cys Val His Phe Val Ala Glu Glu Gln Asp 
        115                 120                 


<210>  7
<211>  1000
<212>  DNA
<213>  Phaeodactylum tricornutum

<400>  7
ggatttgcct cccatgcgcg gaaagtttgc acagagccag ctacagcaat gtcaatttct       60

tttgcagtgg ttggcacggg ttgatgggct gcactatcga tattgctgtc aatggctgtg      120

ctttggtttg acatcggtgc tgtgcgacgt ttggccatgc gctcggccat tctgtttttt      180

cgaatatgca aagttgtctc ttcccgagat cgacgaccgt cttcagctga cacggtcttc      240

ttaaatgacg catcacgacg aggaactaaa gccgcccagg tatacaattg tggcattaga      300

gactgaatac aatgcctcga atagcggaga tactaagggc cgttatttcg tacctgcggc      360

gactagggtc atgattgtat ctctaagaac aacaagggaa atttctgatc aaggtcgacg      420

ggtaaaaggc ggaacaagaa taaaaggatg gtgatacgga acagagcaac gctacagaaa      480

agtgaggatc gccaaccatc aagttgtggc gatgcgatac tttttgcgat aacgtctcgc      540

gctctatgat tttctttgtt atattaattt gttcaacatg agctaattaa ccgaaacctt      600

atgcctcaac tgccgactca gcacaagtac ctaactttgc aaggttttgt cgtatacgtc      660

tgtccataga acgttgacta atgtaagagg aagatttttg tggacgttgt gcgcttgaca      720

tccattggtt gatgtggttt tgctgatgtc acggcatcct gagtccccta acgtttcact      780

tggcgcctcg cagctgtttg cagttcgctc ggtatctttt tctaggatct tccgcagatt      840

tgaagttcgc atcgaaacca ttcctgtcgt ggaaaaaagg cctggatcgc atcttgcagg      900

tgcaacgcta attcttctcc attcataaaa acagaactcg tagaaaacga ttcaaatctt      960

ttttcgcctt tctaaacatc agtaattcta tcaaattcta                           1000


<210>  8
<211>  479
<212>  DNA
<213>  Thalassiosira pseudonana

<400>  8
tcactctgtc gcgctgttgg cgccactact ttgggggtac gagtttaggc tgccttggct       60

gggataaaga atgataagtt tacataattt gtattggaaa tccatcgagt tttggattca      120

gttgacgtct cctgcgttac tatgtcttca ttctctccag tatcaatgcc tatggttcgt      180

cgacattgag cacatttctt tcatcagcgc gatgcatgca atcatcactt cgcaatcttg      240

acaaacatcc tcaatgattc ctccacctct cccaacaaag tcaatgcatt catccttgga      300

tcttctcctc caccgaacgg ccgtgaagcc gactccatta gtgcatccag tccatcaaaa      360

taccgtatga attcccgaaa agattcactt gccaagtact gtttgtcatc ctcctcttca      420

ggtatctcat caatgagtgc atttgcagct atacgaatct ttgactcgga aatcaatcc       479


<210>  9
<211>  203
<212>  DNA
<213>  Simian virus 40

<400>  9
aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc       60

agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc gatggggcgg      120

agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat      180

ggttgctgac taattgagat gca                                              203


<210>  10
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric intron

<400>  10
ctgtggagag aaaggcaaag tggatgtcag taagaccaat aggtgcctat cagaaacgca       60

agagtcttct ctgtctcgac aagcccagtt tctattggtc tccttaaacc tgtcttgtaa      120

ccttgatact tac                                                         133


<210>  11
<211>  222
<212>  DNA
<213>  Simian virus 40

<400>  11
taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct       60

gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta      120

caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag      180

ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tg                         222


<210>  12
<211>  243
<212>  DNA
<213>  Simian virus 40

<400>  12
catatggctg tggaatgagt gtgtcagtgg gtgtggaaag tccccaggct ccccagcagg       60

cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg      120

ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc      180

gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgctcca      240

tcg                                                                    243


<210>  13
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MsDGAT2 F2

<400>  13
caagcttgcc gccaacatgg tgacgtggct caacc                                  35


<210>  14
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MsDGAT2 R1

<400>  14
gccccgactc tagaagatct ttattacaac tcgtcgtcgt tgac                        44


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MsDGAT2 geno F1

<400>  15
cccggaacta catctttggc tac                                               23


<210>  16
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MsDGAT2 geno R1

<400>  16
gtggaaaatg cagggagcga ac                                                22


<210>  17
<211>  1194
<212>  DNA
<213>  Nannochloropsis gaditana

<400>  17
atggtgcttg gggagggcaa aaaaactgat ggcaagtcga ccgatccagc aaaagcgacc       60

gaggagaagc gcgtccccgc cccgctgcga gagcgcaaag ggaagaagca ggaagggaaa      120

gacttgcgaa ccccatctag caacctgaaa cctgcgcgtt cccccacgga ggtcgattgg      180

agttcttttc ccgagggaag ctacacacgt tttgggcatg gtggggattg gtggaccttc      240

ttgaaaggca cacttgccat tttaacagtg tgggcaacct ggttaggggg gggcacttca      300

cccgtatgga tggcttgttt atacatgcat gggtaccgag ttgcattctg gatcctgttg      360

ggccctttac tttatccgct tttccttcct gtcccagcct ggcctgcatt tgttcgtttc      420

gtgttggaca tggccggtta ttttgagggt ggggccgcca tgtacgtcga gaattctttc      480

aagggcagga atgtcaacgg tccaatcatg ctggccatgc atccccatgg gatcatgccg      540

cactccttcc ttttgaacgg cgcaggccgg attcatgccc aaaaacccga attttacctc      600

cccccccact accaagacat gtccctgaaa tcgacggggg tggcggaacc gctcctcttt      660

cgcatccctt tgatatctgc ctttctgtac ttttttggat gcgcggagcc ggcgtcgaag      720

gcgatgatgc acaacatctt ggagcggcag gtgccttttg gcattttggt gggaggctcg      780

gaggagattt tgctgatgga gtatcaacga gagaatgtct acattttgga gcgaaaaggc      840

ttcattaaat acgcgttaca gcacggctat actatcgccc tggggtattt gttcggagag      900

agcaacctgt accacaccat cacctggggc cggaagctcc ggcttgccct tttcaagcgc      960

ttcaagatcc caatcttctt cgcccgggga cgctggtttt tccccctact gcccgaacgg     1020

gccacccccc ttaacgccgt ggtcggaaac cccatcgacc tgcctcggat cgccgacccg     1080

acccaggagg acatagacaa ataccacgcg atctatatcg agaagctcac ggacttattt     1140

gagcggaata aggctgcctt tggatacccg gaccgggagc tgcatctgtt ttga           1194


<210>  18
<211>  397
<212>  PRT
<213>  Nannochloropsis gaditana

<400>  18

Met Val Leu Gly Glu Gly Lys Lys Thr Asp Gly Lys Ser Thr Asp Pro 
1               5                   10                  15      


Ala Lys Ala Thr Glu Glu Lys Arg Val Pro Ala Pro Leu Arg Glu Arg 
            20                  25                  30          


Lys Gly Lys Lys Gln Glu Gly Lys Asp Leu Arg Thr Pro Ser Ser Asn 
        35                  40                  45              


Leu Lys Pro Ala Arg Ser Pro Thr Glu Val Asp Trp Ser Ser Phe Pro 
    50                  55                  60                  


Glu Gly Ser Tyr Thr Arg Phe Gly His Gly Gly Asp Trp Trp Thr Phe 
65                  70                  75                  80  


Leu Lys Gly Thr Leu Ala Ile Leu Thr Val Trp Ala Thr Trp Leu Gly 
                85                  90                  95      


Gly Gly Thr Ser Pro Val Trp Met Ala Cys Leu Tyr Met His Gly Tyr 
            100                 105                 110         


Arg Val Ala Phe Trp Ile Leu Leu Gly Pro Leu Leu Tyr Pro Leu Phe 
        115                 120                 125             


Leu Pro Val Pro Ala Trp Pro Ala Phe Val Arg Phe Val Leu Asp Met 
    130                 135                 140                 


Ala Gly Tyr Phe Glu Gly Gly Ala Ala Met Tyr Val Glu Asn Ser Phe 
145                 150                 155                 160 


Lys Gly Arg Asn Val Asn Gly Pro Ile Met Leu Ala Met His Pro His 
                165                 170                 175     


Gly Ile Met Pro His Ser Phe Leu Leu Asn Gly Ala Gly Arg Ile His 
            180                 185                 190         


Ala Gln Lys Pro Glu Phe Tyr Leu Pro Pro His Tyr Gln Asp Met Ser 
        195                 200                 205             


Leu Lys Ser Thr Gly Val Ala Glu Pro Leu Leu Phe Arg Ile Pro Leu 
    210                 215                 220                 


Ile Ser Ala Phe Leu Tyr Phe Phe Gly Cys Ala Glu Pro Ala Ser Lys 
225                 230                 235                 240 


Ala Met Met His Asn Ile Leu Glu Arg Gln Val Pro Phe Gly Ile Leu 
                245                 250                 255     


Val Gly Gly Ser Glu Glu Ile Leu Leu Met Glu Tyr Gln Arg Glu Asn 
            260                 265                 270         


Val Tyr Ile Leu Glu Arg Lys Gly Phe Ile Lys Tyr Ala Leu Gln His 
        275                 280                 285             


Gly Tyr Thr Ile Ala Leu Gly Tyr Leu Phe Gly Glu Ser Asn Leu Tyr 
    290                 295                 300                 


His Thr Ile Thr Trp Gly Arg Lys Leu Arg Leu Ala Leu Phe Lys Arg 
305                 310                 315                 320 


Phe Lys Ile Pro Ile Phe Phe Ala Arg Gly Arg Trp Phe Phe Pro Leu 
                325                 330                 335     


Leu Pro Glu Arg Ala Thr Pro Leu Asn Ala Val Val Gly Asn Pro Ile 
            340                 345                 350         


Asp Leu Pro Arg Ile Ala Asp Pro Thr Gln Glu Asp Ile Asp Lys Tyr 
        355                 360                 365             


His Ala Ile Tyr Ile Glu Lys Leu Thr Asp Leu Phe Glu Arg Asn Lys 
    370                 375                 380                 


Ala Ala Phe Gly Tyr Pro Asp Arg Glu Leu His Leu Phe 
385                 390                 395         


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 7DGAT F1

<400>  19
atggtgcttg gggagggcaa a                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7DGAT R1

<400>  20
cttcaaaaca gatgcagctc c                                                 21


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7DGAT F2

<400>  21
caagcttgcc gccaacatgg tgcttgggga gggcaaa                                37


<210>  22
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7DGAT R2

<400>  22
gccccgactc tagaagatct cttcaaaaca gatgcagctc c                           41


<210>  23
<211>  1446
<212>  DNA
<213>  Nannochloropsis gaditana

<400>  23
atgactgcct tctttcggcg taggaatggg agtgggagca gcacaagcag cagttcctcc       60

tccttggcag aagacaagca ctccatcctt tcctctgaaa tcgatccact cccttgttcg      120

ccctccacca ccgccagcac atggaaaggg cccgacgtgg cgaacaaggc gagggagtcg      180

acctgccttg ctccatcgca ctccacaaca agccaagtcg ctgctgtacc tggacagaac      240

ggggccattg ccactaccga agacgcctcc aagtctgaca tacgacacct acaaagaccg      300

ccccaggatc cccttaccga gcatattctc gacacaggaa acaaaggcac ggcaggacaa      360

ggacaccgcc ggcgcacgtc cgcgacgctc tcatcctggg cttctttttc ctcttcgaat      420

accctttcct tcttgacggg cgaggactcg tccatagtcg cccccgtccg ggggcctgcc      480

aagctggatt tctccacgcc cgtccaccca gccacgcaag ccgttgattt cgtcatcacc      540

ttcctcctcg tgcattacat ccaagtcttt tactctctcg tcttcctctt tatctacctg      600

gtccggcacg gctaccgttg gccccttgtc ctggccggcc tctacctccc ttcctatttt      660

atccccttcc aacgcctggg ggggtggccc ttccgccttc tcatgcggcg ccccttctgg      720

cgctgtgtcc agcgcacgct cgctttcaaa gtggaacggg aggtggaact ctcgcccgcc      780

acccagtaca tcttcggctg gcacccccac ggcatcctcc ttctctcccg ctttgccatc      840

tacgggggcc tctgggagaa gctcttcccg ggcgtgcact tcaagacctt ggccgccagc      900

cccttgttct ggatcccgcc catccgggag gtatcgatcc tcttgggggg cgtggacgcc      960

gggagggcct ctgccaatcg ggccttgcgg gacggctact ccgtctccct ttacccgggg     1020

gggagcaagg agatctacac gacgaacccg tacacgcccg agaccacact ggtgctcaag     1080

attcggaagg gcttcattcg catggccctc cggtacggat gccccctggt ccctgtctac     1140

acctttggag agaagtacgc ctaccatcgt ctcggtcacg ccaccggctt cgcccactgg     1200

ctcctggcgg tgctgcgggt gccttttctg attttctggg gacgctggtg cacctttatg     1260

cctctgaagc acacgcaggt gtcggtggtg gtagggaagc ccctcccagt ccccaagatc     1320

gagggggaac ccgcgcctga ggtggtggag gagtggctgc agcggtactg cgatgaggtg     1380

caggcactct tccaacggca taaacacaag tacgcacgcc cggaagagtt cgtggcgatt     1440

tcgtga                                                                1446


<210>  24
<211>  481
<212>  PRT
<213>  Nannochloropsis gaditana

<400>  24

Met Thr Ala Phe Phe Arg Arg Arg Asn Gly Ser Gly Ser Ser Thr Ser 
1               5                   10                  15      


Ser Ser Ser Ser Ser Leu Ala Glu Asp Lys His Ser Ile Leu Ser Ser 
            20                  25                  30          


Glu Ile Asp Pro Leu Pro Cys Ser Pro Ser Thr Thr Ala Ser Thr Trp 
        35                  40                  45              


Lys Gly Pro Asp Val Ala Asn Lys Ala Arg Glu Ser Thr Cys Leu Ala 
    50                  55                  60                  


Pro Ser His Ser Thr Thr Ser Gln Val Ala Ala Val Pro Gly Gln Asn 
65                  70                  75                  80  


Gly Ala Ile Ala Thr Thr Glu Asp Ala Ser Lys Ser Asp Ile Arg His 
                85                  90                  95      


Leu Gln Arg Pro Pro Gln Asp Pro Leu Thr Glu His Ile Leu Asp Thr 
            100                 105                 110         


Gly Asn Lys Gly Thr Ala Gly Gln Gly His Arg Arg Arg Thr Ser Ala 
        115                 120                 125             


Thr Leu Ser Ser Trp Ala Ser Phe Ser Ser Ser Asn Thr Leu Ser Phe 
    130                 135                 140                 


Leu Thr Gly Glu Asp Ser Ser Ile Val Ala Pro Val Arg Gly Pro Ala 
145                 150                 155                 160 


Lys Leu Asp Phe Ser Thr Pro Val His Pro Ala Thr Gln Ala Val Asp 
                165                 170                 175     


Phe Val Ile Thr Phe Leu Leu Val His Tyr Ile Gln Val Phe Tyr Ser 
            180                 185                 190         


Leu Val Phe Leu Phe Ile Tyr Leu Val Arg His Gly Tyr Arg Trp Pro 
        195                 200                 205             


Leu Val Leu Ala Gly Leu Tyr Leu Pro Ser Tyr Phe Ile Pro Phe Gln 
    210                 215                 220                 


Arg Leu Gly Gly Trp Pro Phe Arg Leu Leu Met Arg Arg Pro Phe Trp 
225                 230                 235                 240 


Arg Cys Val Gln Arg Thr Leu Ala Phe Lys Val Glu Arg Glu Val Glu 
                245                 250                 255     


Leu Ser Pro Ala Thr Gln Tyr Ile Phe Gly Trp His Pro His Gly Ile 
            260                 265                 270         


Leu Leu Leu Ser Arg Phe Ala Ile Tyr Gly Gly Leu Trp Glu Lys Leu 
        275                 280                 285             


Phe Pro Gly Val His Phe Lys Thr Leu Ala Ala Ser Pro Leu Phe Trp 
    290                 295                 300                 


Ile Pro Pro Ile Arg Glu Val Ser Ile Leu Leu Gly Gly Val Asp Ala 
305                 310                 315                 320 


Gly Arg Ala Ser Ala Asn Arg Ala Leu Arg Asp Gly Tyr Ser Val Ser 
                325                 330                 335     


Leu Tyr Pro Gly Gly Ser Lys Glu Ile Tyr Thr Thr Asn Pro Tyr Thr 
            340                 345                 350         


Pro Glu Thr Thr Leu Val Leu Lys Ile Arg Lys Gly Phe Ile Arg Met 
        355                 360                 365             


Ala Leu Arg Tyr Gly Cys Pro Leu Val Pro Val Tyr Thr Phe Gly Glu 
    370                 375                 380                 


Lys Tyr Ala Tyr His Arg Leu Gly His Ala Thr Gly Phe Ala His Trp 
385                 390                 395                 400 


Leu Leu Ala Val Leu Arg Val Pro Phe Leu Ile Phe Trp Gly Arg Trp 
                405                 410                 415     


Cys Thr Phe Met Pro Leu Lys His Thr Gln Val Ser Val Val Val Gly 
            420                 425                 430         


Lys Pro Leu Pro Val Pro Lys Ile Glu Gly Glu Pro Ala Pro Glu Val 
        435                 440                 445             


Val Glu Glu Trp Leu Gln Arg Tyr Cys Asp Glu Val Gln Ala Leu Phe 
    450                 455                 460                 


Gln Arg His Lys His Lys Tyr Ala Arg Pro Glu Glu Phe Val Ala Ile 
465                 470                 475                 480 


Ser 
    


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2DGAT F1

<400>  25
atgactgcct tctttcggcg tag                                               23


<210>  26
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2DGAT R1

<400>  26
tcacgaaatc gccacgaact cttc                                              24


<210>  27
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2DGAT F2

<400>  27
caagcttgcc gccaacatga ctgccttctt tcggcgtag                              39


<210>  28
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2DGAT R2

<400>  28
gccccgactc tagaagatct tcacgaaatc gccacgaact cttc                        44


<210>  29
<211>  61
<212>  DNA
<213>  Phaeodactylum tricornutum

<400>  29
atgttcatga gaattgccgt agcagcactg gccttgctgg ctgctccctc cattcgtgcc       60

g                                                                       61


<210>  30
<211>  20
<212>  PRT
<213>  Phaeodactylum tricornutum

<400>  30

Met Phe Met Arg Ile Ala Val Ala Ala Leu Ala Leu Leu Ala Ala Pro 
1               5                   10                  15      


Ser Ile Arg Ala 
            20  


<210>  31
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2DGATgeno F2

<400>  31
gggagtcgac ctgccttg                                                     18


<210>  32
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2DGAT geno R2

<400>  32
gaagacgaga gagtaaaaga cttggatg                                          28


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7DGAT geno F2

<400>  33
catgggatca tgccgcactc                                                   20


<210>  34
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7DGAT geno R2

<400>  34
caaatacccc agggcgatag tatag                                             25


<210>  35
<211>  1005
<212>  DNA
<213>  Nannochloropsis gaditana

<400>  35
atgaaagatg aggaccgctt gacagtcctg aaaaggcttt cgaagccagt agccgaggcc       60

aagcccggag agctaggttt gggacaaatt gagcacatga cagtctggga ggaaattgtg      120

gccgcaacgt tcttgttgtt tgttgtggga agtatgctgt ggctgcctat agccgtggta      180

attttcgcta tcttcgtacg aagtgcggtg gcatgggcca ctatgacagt ggtcttcgtc      240

gccctgagcc tgcaccccgt ttcacgagta cacagcatgg ttcactctcc cttgaactat      300

tttattttca agtatttctc aatgaagatg gcttccgact atcctatcga tagcaaagga      360

cagtatattt tcgtcgcgcc ccctcacgga gtcttgccct tcgggaacct gctgacggtt      420

catgccatga aatcttgtgg agggatggaa ttccgcggtt tgactactga cgtagcttta      480

agacttccta tttttcgaca ctacttaggc ctggttggga ccatcccagc ttcaagacac      540

gtcgcaagga agtacttgga caaaggctgg tcgctaggaa tctccagcgg tggagtcgcg      600

gagatttttg aagtcaacaa caacgatgaa gttatcctta tgaaagagcg caaaggattc      660

gtcaaactcg cccttcaaac cgggactccc ctcgtcccgt cctacatctt tggcaacacc      720

aaactcctct cagcgtggta tgatgatgct ggagcccttc aagggatatc ccgctatttc      780

aaatttggga tactgctact ttggggtcgt tttggtcttc ccctcatgca tcggcatccc      840

gtcttagggg tcatggccaa gccaatcgtt gtgccccgtg cggaaggaga acccacgcag      900

gatatgattg acaccattca tgcccaattc tgcgattcac ttatcgcact ctttgacgat      960

tacaagggct tgtatggttg gcctgacaag aaactcctta ttaag                     1005


<210>  36
<211>  335
<212>  PRT
<213>  Nannochloropsis gaditana

<400>  36

Met Lys Asp Glu Asp Arg Leu Thr Val Leu Lys Arg Leu Ser Lys Pro 
1               5                   10                  15      


Val Ala Glu Ala Lys Pro Gly Glu Leu Gly Leu Gly Gln Ile Glu His 
            20                  25                  30          


Met Thr Val Trp Glu Glu Ile Val Ala Ala Thr Phe Leu Leu Phe Val 
        35                  40                  45              


Val Gly Ser Met Leu Trp Leu Pro Ile Ala Val Val Ile Phe Ala Ile 
    50                  55                  60                  


Phe Val Arg Ser Ala Val Ala Trp Ala Thr Met Thr Val Val Phe Val 
65                  70                  75                  80  


Ala Leu Ser Leu His Pro Val Ser Arg Val His Ser Met Val His Ser 
                85                  90                  95      


Pro Leu Asn Tyr Phe Ile Phe Lys Tyr Phe Ser Met Lys Met Ala Ser 
            100                 105                 110         


Asp Tyr Pro Ile Asp Ser Lys Gly Gln Tyr Ile Phe Val Ala Pro Pro 
        115                 120                 125             


His Gly Val Leu Pro Phe Gly Asn Leu Leu Thr Val His Ala Met Lys 
    130                 135                 140                 


Ser Cys Gly Gly Met Glu Phe Arg Gly Leu Thr Thr Asp Val Ala Leu 
145                 150                 155                 160 


Arg Leu Pro Ile Phe Arg His Tyr Leu Gly Leu Val Gly Thr Ile Pro 
                165                 170                 175     


Ala Ser Arg His Val Ala Arg Lys Tyr Leu Asp Lys Gly Trp Ser Leu 
            180                 185                 190         


Gly Ile Ser Ser Gly Gly Val Ala Glu Ile Phe Glu Val Asn Asn Asn 
        195                 200                 205             


Asp Glu Val Ile Leu Met Lys Glu Arg Lys Gly Phe Val Lys Leu Ala 
    210                 215                 220                 


Leu Gln Thr Gly Thr Pro Leu Val Pro Ser Tyr Ile Phe Gly Asn Thr 
225                 230                 235                 240 


Lys Leu Leu Ser Ala Trp Tyr Asp Asp Ala Gly Ala Leu Gln Gly Ile 
                245                 250                 255     


Ser Arg Tyr Phe Lys Phe Gly Ile Leu Leu Leu Trp Gly Arg Phe Gly 
            260                 265                 270         


Leu Pro Leu Met His Arg His Pro Val Leu Gly Val Met Ala Lys Pro 
        275                 280                 285             


Ile Val Val Pro Arg Ala Glu Gly Glu Pro Thr Gln Asp Met Ile Asp 
    290                 295                 300                 


Thr Ile His Ala Gln Phe Cys Asp Ser Leu Ile Ala Leu Phe Asp Asp 
305                 310                 315                 320 


Tyr Lys Gly Leu Tyr Gly Trp Pro Asp Lys Lys Leu Leu Ile Lys 
                325                 330                 335 


<210>  37
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ab_AtfA1 _ GLR001_PciI_F

<400>  37
agcggccgca agcttgccgc caaatgaaag ccttaagtcc agtgg                       45


<210>  38
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ab_AtfA1 _GLR001_PciI_R

<400>  38
gaccgacgta cctctgagtg acatgctatt tgatcccagc tccaatc                     47


<210>  39
<211>  1374
<212>  DNA
<213>  Alcanivorax borkumensis

<400>  39
atgaaagcgc ttagcccagt ggatcaactg ttcctgtggc tggaaaaacg acagcaaccc       60

atgcacgtag gcggtttgca gctgttttcc ttcccggaag gtgccggccc caagtatgtg      120

agtgagctgg cccagcaaat gcgggattac tgccacccag tggcgccatt caaccagcgc      180

ctgacccgtc gactcggcca gtattactgg actagagaca aacagttcga tatcgaccac      240

cacttccgcc acgaagcact ccccaaaccc ggtcgcattc gcgaactgct ttctttggtc      300

tccgccgaac attccaacct gctggaccgg gagcgcccca tgtgggaagc ccatttgatc      360

gaagggatcc gcggtcgcca gttcgctctc tattataaga tccaccattc ggtgatggat      420

ggcatatccg ccatgcgtat cgcctccaaa acgctttcca ctgaccccag tgaacgtgaa      480

atggctccgg cttgggcgtt caacaccaaa aaacgctccc gctcactgcc cagcaacccg      540

gttgacatgg cctccagcat ggcgcgccta accgcgagca taagcaaaca agctgccaca      600

gtgcccggtc tcgcgcggga ggtttacaaa gtcacccaaa aagccaaaaa agatgaaaac      660

tatgtgtcta tttttcaggc tcccgacacg attctgaata ataccatcac cggttcacgc      720

cgctttgccg cccagagctt tccattaccg cgcctgaaag ttatcgccaa ggcctataac      780

tgcaccatta acaccgtggt gctctccatg tgtggccacg ctctgcgcga atacttgatt      840

agccaacacg cgctgcccga tgagccactg attgccatgg tgcccatgag cctgcggcag      900

gacgacagca ctggcggcaa ccagatcggt atgatcttgg ctaacctggg cacccacatc      960

tgtgatccag ctaatcgcct gcgcgtcatc cacgattccg tcgaggaagc caaatcccgc     1020

ttctcgcaga tgagcccgga agaaattctc aatttcaccg ccctcaccat ggctcccacc     1080

ggcttgaact tactgaccgg cctagcgcca aaatggcggg ccttcaacgt ggtgatttcc     1140

aacatacccg ggccgaaaga gccgctgtac tggaatggtg cacagctgca aggagtgtat     1200

ccagtatcca ttgccttgga tcgcatcgcc ctaaatatca ccctcaccag ttatgtagac     1260

cagatggaat ttgggcttat cgcctgccgc cgtactctgc cttccatgca gcgactactg     1320

gattacctgg aacagtccat ccgcgaattg gaaatcggtg caggaattaa atag           1374


<210>  40
<211>  457
<212>  PRT
<213>  Alcanivorax borkumensis

<400>  40

Met Lys Ala Leu Ser Pro Val Asp Gln Leu Phe Leu Trp Leu Glu Lys 
1               5                   10                  15      


Arg Gln Gln Pro Met His Val Gly Gly Leu Gln Leu Phe Ser Phe Pro 
            20                  25                  30          


Glu Gly Ala Gly Pro Lys Tyr Val Ser Glu Leu Ala Gln Gln Met Arg 
        35                  40                  45              


Asp Tyr Cys His Pro Val Ala Pro Phe Asn Gln Arg Leu Thr Arg Arg 
    50                  55                  60                  


Leu Gly Gln Tyr Tyr Trp Thr Arg Asp Lys Gln Phe Asp Ile Asp His 
65                  70                  75                  80  


His Phe Arg His Glu Ala Leu Pro Lys Pro Gly Arg Ile Arg Glu Leu 
                85                  90                  95      


Leu Ser Leu Val Ser Ala Glu His Ser Asn Leu Leu Asp Arg Glu Arg 
            100                 105                 110         


Pro Met Trp Glu Ala His Leu Ile Glu Gly Ile Arg Gly Arg Gln Phe 
        115                 120                 125             


Ala Leu Tyr Tyr Lys Ile His His Ser Val Met Asp Gly Ile Ser Ala 
    130                 135                 140                 


Met Arg Ile Ala Ser Lys Thr Leu Ser Thr Asp Pro Ser Glu Arg Glu 
145                 150                 155                 160 


Met Ala Pro Ala Trp Ala Phe Asn Thr Lys Lys Arg Ser Arg Ser Leu 
                165                 170                 175     


Pro Ser Asn Pro Val Asp Met Ala Ser Ser Met Ala Arg Leu Thr Ala 
            180                 185                 190         


Ser Ile Ser Lys Gln Ala Ala Thr Val Pro Gly Leu Ala Arg Glu Val 
        195                 200                 205             


Tyr Lys Val Thr Gln Lys Ala Lys Lys Asp Glu Asn Tyr Val Ser Ile 
    210                 215                 220                 


Phe Gln Ala Pro Asp Thr Ile Leu Asn Asn Thr Ile Thr Gly Ser Arg 
225                 230                 235                 240 


Arg Phe Ala Ala Gln Ser Phe Pro Leu Pro Arg Leu Lys Val Ile Ala 
                245                 250                 255     


Lys Ala Tyr Asn Cys Thr Ile Asn Thr Val Val Leu Ser Met Cys Gly 
            260                 265                 270         


His Ala Leu Arg Glu Tyr Leu Ile Ser Gln His Ala Leu Pro Asp Glu 
        275                 280                 285             


Pro Leu Ile Ala Met Val Pro Met Ser Leu Arg Gln Asp Asp Ser Thr 
    290                 295                 300                 


Gly Gly Asn Gln Ile Gly Met Ile Leu Ala Asn Leu Gly Thr His Ile 
305                 310                 315                 320 


Cys Asp Pro Ala Asn Arg Leu Arg Val Ile His Asp Ser Val Glu Glu 
                325                 330                 335     


Ala Lys Ser Arg Phe Ser Gln Met Ser Pro Glu Glu Ile Leu Asn Phe 
            340                 345                 350         


Thr Ala Leu Thr Met Ala Pro Thr Gly Leu Asn Leu Leu Thr Gly Leu 
        355                 360                 365             


Ala Pro Lys Trp Arg Ala Phe Asn Val Val Ile Ser Asn Ile Pro Gly 
    370                 375                 380                 


Pro Lys Glu Pro Leu Tyr Trp Asn Gly Ala Gln Leu Gln Gly Val Tyr 
385                 390                 395                 400 


Pro Val Ser Ile Ala Leu Asp Arg Ile Ala Leu Asn Ile Thr Leu Thr 
                405                 410                 415     


Ser Tyr Val Asp Gln Met Glu Phe Gly Leu Ile Ala Cys Arg Arg Thr 
            420                 425                 430         


Leu Pro Ser Met Gln Arg Leu Leu Asp Tyr Leu Glu Gln Ser Ile Arg 
        435                 440                 445             


Glu Leu Glu Ile Gly Ala Gly Ile Lys 
    450                 455         


<210>  41
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Mh_WS1_GLR001_PciI_F

<400>  41
agcggccgca agcttgccgc caaatgacgc ccctgaatcc cactgac                     47


<210>  42
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mh_WS1_GLR001_PciI_R

<400>  42
gaccgacgta cctctgagtg acatgttaca gaccggcgtt gagctcc                     47


<210>  43
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WS1_416TEF_F

<400>  43
tctaatctaa gttttctaga actagtggat cccccgccac catgacgccc ctgaatccca       60

ctgac                                                                   65


<210>  44
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WS1_416TEF

<400>  44
gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccttact attacagacc       60

ggcgttgagc tcc                                                          73


<210>  45
<211>  1368
<212>  DNA
<213>  Marinobacter hydrocarbonoclasticus

<400>  45
atgacgcccc tgaatcccac tgaccagctc tttctctggc tggaaaaacg ccagcagccc       60

atgcatgtgg gcggcctcca gctgttttcc ttccccgaag gcgcgccgga cgactatgtc      120

gcgcagctgg cagaccagct tcggcagaag acggaggtga ccgccccctt taaccagcgc      180

ctgagctatc gcctgggcca gccggtatgg gtggaggatg agcacctgga ccttgagcat      240

catttccgct tcgaggcgct gcccacaccc gggcgtattc gggagctgct gtcgttcgta      300

tcggcggagc attcgcacct gatggaccgg gagcgcccca tgtgggaggt gcacctgatc      360

gagggcctga aagaccggca gtttgcgctc tacaccaagg ttcaccattc cctggtggac      420

ggtgtctcgg ccatgcgcat ggccacccgg atgctgagtg aaaacccgga cgaacacggc      480

atgccgccaa tctgggatct gccttgcctg tcacgggata ggggtgagtc ggacggacac      540

tccctctggc gcagtgtcac ccatttgctg gggctttcgg gccgccagct cggcaccatt      600

cccactgtgg caaaggagct actgaaaacc atcaatcagg cccggaagga tccggcctac      660

gactccattt tccatgcccc gcgctgcatg ctgaaccaga aaatcaccgg ttcccgtcgt      720

ttcgccgccc agtcctggtg cctgaaacgg attcgcgccg tgtgcgaggc ctatggcacc      780

acggtcaacg atgtcgtaac tgccatgtgc gcagcggctc tgcgtaccta tctgatgaat      840

caggatgcct tgccggagaa accactggtg gcctttgtgc cggtgtcact acgccgggac      900

gacagctccg ggggcaacca ggtaggcgtc atcctggcga gccttcacac cgatgtgcag      960

gaggccggcg aacgactgtt aaaaatccac catggcatgg aagaggccaa gcagcgctac     1020

cgtcatatga gcccggagga aatcgtcaac tacacggccc tgaccctggc gccggccgcc     1080

ttccacctgc tgaccgggct ggcgcccaag tggcagacct tcaatgtggt gatttccaat     1140

gtccccgggc catccaggcc cctgtactgg aacggggcga aactggaagg catgtatccg     1200

gtgtctatcg atatggacag actggccctg aacatgacac tgaccagcta taacgaccag     1260

gtggagttcg gcctgattgg ctgtcgccgg accctgccca gcctgcaacg gatgctggac     1320

tacctggaac agggtctggc agagctggag ctcaacgccg gtctgtaa                  1368


<210>  46
<211>  455
<212>  PRT
<213>  Marinobacter hydrocarbonoclasticus

<400>  46

Met Thr Pro Leu Asn Pro Thr Asp Gln Leu Phe Leu Trp Leu Glu Lys 
1               5                   10                  15      


Arg Gln Gln Pro Met His Val Gly Gly Leu Gln Leu Phe Ser Phe Pro 
            20                  25                  30          


Glu Gly Ala Pro Asp Asp Tyr Val Ala Gln Leu Ala Asp Gln Leu Arg 
        35                  40                  45              


Gln Lys Thr Glu Val Thr Ala Pro Phe Asn Gln Arg Leu Ser Tyr Arg 
    50                  55                  60                  


Leu Gly Gln Pro Val Trp Val Glu Asp Glu His Leu Asp Leu Glu His 
65                  70                  75                  80  


His Phe Arg Phe Glu Ala Leu Pro Thr Pro Gly Arg Ile Arg Glu Leu 
                85                  90                  95      


Leu Ser Phe Val Ser Ala Glu His Ser His Leu Met Asp Arg Glu Arg 
            100                 105                 110         


Pro Met Trp Glu Val His Leu Ile Glu Gly Leu Lys Asp Arg Gln Phe 
        115                 120                 125             


Ala Leu Tyr Thr Lys Val His His Ser Leu Val Asp Gly Val Ser Ala 
    130                 135                 140                 


Met Arg Met Ala Thr Arg Met Leu Ser Glu Asn Pro Asp Glu His Gly 
145                 150                 155                 160 


Met Pro Pro Ile Trp Asp Leu Pro Cys Leu Ser Arg Asp Arg Gly Glu 
                165                 170                 175     


Ser Asp Gly His Ser Leu Trp Arg Ser Val Thr His Leu Leu Gly Leu 
            180                 185                 190         


Ser Gly Arg Gln Leu Gly Thr Ile Pro Thr Val Ala Lys Glu Leu Leu 
        195                 200                 205             


Lys Thr Ile Asn Gln Ala Arg Lys Asp Pro Ala Tyr Asp Ser Ile Phe 
    210                 215                 220                 


His Ala Pro Arg Cys Met Leu Asn Gln Lys Ile Thr Gly Ser Arg Arg 
225                 230                 235                 240 


Phe Ala Ala Gln Ser Trp Cys Leu Lys Arg Ile Arg Ala Val Cys Glu 
                245                 250                 255     


Ala Tyr Gly Thr Thr Val Asn Asp Val Val Thr Ala Met Cys Ala Ala 
            260                 265                 270         


Ala Leu Arg Thr Tyr Leu Met Asn Gln Asp Ala Leu Pro Glu Lys Pro 
        275                 280                 285             


Leu Val Ala Phe Val Pro Val Ser Leu Arg Arg Asp Asp Ser Ser Gly 
    290                 295                 300                 


Gly Asn Gln Val Gly Val Ile Leu Ala Ser Leu His Thr Asp Val Gln 
305                 310                 315                 320 


Glu Ala Gly Glu Arg Leu Leu Lys Ile His His Gly Met Glu Glu Ala 
                325                 330                 335     


Lys Gln Arg Tyr Arg His Met Ser Pro Glu Glu Ile Val Asn Tyr Thr 
            340                 345                 350         


Ala Leu Thr Leu Ala Pro Ala Ala Phe His Leu Leu Thr Gly Leu Ala 
        355                 360                 365             


Pro Lys Trp Gln Thr Phe Asn Val Val Ile Ser Asn Val Pro Gly Pro 
    370                 375                 380                 


Ser Arg Pro Leu Tyr Trp Asn Gly Ala Lys Leu Glu Gly Met Tyr Pro 
385                 390                 395                 400 


Val Ser Ile Asp Met Asp Arg Leu Ala Leu Asn Met Thr Leu Thr Ser 
                405                 410                 415     


Tyr Asn Asp Gln Val Glu Phe Gly Leu Ile Gly Cys Arg Arg Thr Leu 
            420                 425                 430         


Pro Ser Leu Gln Arg Met Leu Asp Tyr Leu Glu Gln Gly Leu Ala Glu 
        435                 440                 445             


Leu Glu Leu Asn Ala Gly Leu 
    450                 455 


<210>  47
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ms_WS_DGAT _GLR001_PciI_F

<400>  47
agcggccgca agcttgccgc caaatgaacc gcatgcaact c                           41


<210>  48
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ms_WS_DGAT _GLR001_PciI_R

<400>  48
gaccgacgta cctctgagtg acatgttaag cgccagtggc ggtttc                      46


<210>  49
<211>  1374
<212>  DNA
<213>  Mycobacterium smegmatis

<400>  49
atgaaccgga tgcagctcat gtcgccgacc gattccatgt tcttgatcgc ggagtcgcgg       60

gagcacccga tgcatgtggg cggtctggcg ctgtacgacc cgcccgacga tgcaggcccg      120

gagttcgtgc gtgagttgta cgaagagatg gtcaggcaca ccgacttcca gccggtgttc      180

cgcaagcatc cggcgaccct gctcgggggt atcgccaacg tgggctggac tctcgacgac      240

gaggtcgacc tcgactacca cctgcggcgt tcggcgctgc cgtcgccggg acgcccgcgc      300

gagcttctcg aactgacctc acgtgtgcac ggcacgctgc tcgaccggca tcgcccgctg      360

tgggaggcct acctcatcga gggtatggcc gacggacgct tcgcggtgta caccaaggtg      420

caccactcgc tgatcgacgg ggtgtcggcg atgaagctcg tcgaacgcac actgtccgag      480

gacccgagcg acaccacggt ccgggtgccg tggaacctgc cgcggcgcga atcgtcgcgc      540

agggcgggct cgtcgtcgtt ggcgcgcacc gcgaccggcg ccgcgacgtc actggcggcg      600

ctggcgccgt cgacgatcag gctggcgcgt gcggcgctgc tcgaacagca gctcacgttg      660

ccgttcggtg cgccaaggac catgttcaac gtcaagatcg gcggggcccg ccgcgtcgcg      720

gcgcagtcgt ggccgctgga gcggttgcgt cgcatcaagg cggtcaccgg ggcaacgatc      780

aacgacatcg tgctcgcgat gtgtgcgggt gcgctgcgcg cctatctcgc cgagcaggac      840

gcgctgccgg accggccgct gatcgcgatg gtgccggtga gcatgcgcag tgaacacgag      900

gccgacgccg gcggcaacat ggtcggctcg atcctgtgca gcctgggcac cgacgtggag      960

gatccggccg accggctcgc ggtgatccgc cgctcgatca ccgacaacaa gagggtgttc     1020

tccgaactgc cgcgcctgca ggcgctcgcg ttgtcggcgc tgctgatcgc cccgctcggg     1080

ctcaccacgg cgttccccgg attcgtcgac gccaccgcgc cgccgttcaa cctcgtgatc     1140

tccaacgtgc cgggacccaa gaagccgctg tactggcgtg gggcccgcct ggcgggcaac     1200

tacccgctgt cgatcgcgct ggacgggcag gccctcaaca tgaccgtggt gagcaacgcc     1260

cacaacctgg atttcgggtt ggtgggctgc cggcgcagcg tgccgcatct gcagcggctg     1320

ctgggccacc tggagacctc gctgaaagac ctcgagacgg ccacaggcgc ctga           1374


<210>  50
<211>  457
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  50

Met Asn Arg Met Gln Leu Met Ser Pro Thr Asp Ser Met Phe Leu Ile 
1               5                   10                  15      


Ala Glu Ser Arg Glu His Pro Met His Val Gly Gly Leu Ala Leu Tyr 
            20                  25                  30          


Asp Pro Pro Asp Asp Ala Gly Pro Glu Phe Val Arg Glu Leu Tyr Glu 
        35                  40                  45              


Glu Met Val Arg His Thr Asp Phe Gln Pro Val Phe Arg Lys His Pro 
    50                  55                  60                  


Ala Thr Leu Leu Gly Gly Ile Ala Asn Val Gly Trp Thr Leu Asp Asp 
65                  70                  75                  80  


Glu Val Asp Leu Asp Tyr His Leu Arg Arg Ser Ala Leu Pro Ser Pro 
                85                  90                  95      


Gly Arg Pro Arg Glu Leu Leu Glu Leu Thr Ser Arg Val His Gly Thr 
            100                 105                 110         


Leu Leu Asp Arg His Arg Pro Leu Trp Glu Ala Tyr Leu Ile Glu Gly 
        115                 120                 125             


Met Ala Asp Gly Arg Phe Ala Val Tyr Thr Lys Val His His Ser Leu 
    130                 135                 140                 


Ile Asp Gly Val Ser Ala Met Lys Leu Val Glu Arg Thr Leu Ser Glu 
145                 150                 155                 160 


Asp Pro Ser Asp Thr Thr Val Arg Val Pro Trp Asn Leu Pro Arg Arg 
                165                 170                 175     


Glu Ser Ser Arg Arg Ala Gly Ser Ser Ser Leu Ala Arg Thr Ala Thr 
            180                 185                 190         


Gly Ala Ala Thr Ser Leu Ala Ala Leu Ala Pro Ser Thr Ile Arg Leu 
        195                 200                 205             


Ala Arg Ala Ala Leu Leu Glu Gln Gln Leu Thr Leu Pro Phe Gly Ala 
    210                 215                 220                 


Pro Arg Thr Met Phe Asn Val Lys Ile Gly Gly Ala Arg Arg Val Ala 
225                 230                 235                 240 


Ala Gln Ser Trp Pro Leu Glu Arg Leu Arg Arg Ile Lys Ala Val Thr 
                245                 250                 255     


Gly Ala Thr Ile Asn Asp Ile Val Leu Ala Met Cys Ala Gly Ala Leu 
            260                 265                 270         


Arg Ala Tyr Leu Ala Glu Gln Asp Ala Leu Pro Asp Arg Pro Leu Ile 
        275                 280                 285             


Ala Met Val Pro Val Ser Met Arg Ser Glu His Glu Ala Asp Ala Gly 
    290                 295                 300                 


Gly Asn Met Val Gly Ser Ile Leu Cys Ser Leu Gly Thr Asp Val Glu 
305                 310                 315                 320 


Asp Pro Ala Asp Arg Leu Ala Val Ile Arg Arg Ser Ile Thr Asp Asn 
                325                 330                 335     


Lys Arg Val Phe Ser Glu Leu Pro Arg Leu Gln Ala Leu Ala Leu Ser 
            340                 345                 350         


Ala Leu Leu Ile Ala Pro Leu Gly Leu Thr Thr Ala Phe Pro Gly Phe 
        355                 360                 365             


Val Asp Ala Thr Ala Pro Pro Phe Asn Leu Val Ile Ser Asn Val Pro 
    370                 375                 380                 


Gly Pro Lys Lys Pro Leu Tyr Trp Arg Gly Ala Arg Leu Ala Gly Asn 
385                 390                 395                 400 


Tyr Pro Leu Ser Ile Ala Leu Asp Gly Gln Ala Leu Asn Met Thr Val 
                405                 410                 415     


Val Ser Asn Ala His Asn Leu Asp Phe Gly Leu Val Gly Cys Arg Arg 
            420                 425                 430         


Ser Val Pro His Leu Gln Arg Leu Leu Gly His Leu Glu Thr Ser Leu 
        435                 440                 445             


Lys Asp Leu Glu Thr Ala Thr Gly Ala 
    450                 455         


<210>  51
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ro_Atf1_416TEF_F

<400>  51
tctaatctaa gttttctaga actagtggat cccccgccac catgacccag acggacttca       60

tgtcg                                                                   65


<210>  52
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ro_Atf1_416TEF_R

<400>  52
gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccttact atcacgaggc       60

cacgaccacc                                                              70


<210>  53
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ro_Atf1_GLR001_PciI_F

<400>  53
agcggccgca agcttgccgc caaatgaccc agacggactt catg                        44


<210>  54
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Ro_Atf1_GLR001_PciI_R

<400>  54
gaccgacgta cctctgagtg acatgtcacg aggccacgac caccctc                     47


<210>  55
<211>  1386
<212>  DNA
<213>  Rhodococcus opacus

<400>  55
atgaatcgag tggccatgag cggcgcaacc gagcgatcga tgacgcagac cgacctgatg       60

tcttggcgga tggaggccga tccggtcctc cgctcgacaa tcgtctcgat catcgtgctg      120

gacaggctcc cggatcacga acgattgacc gccgcgatga accacgccat cgacgtagtt      180

ccgatcttca ggtgcaaagc ggttgcgcga caatttccgt ggagtccacc actttgggtg      240

gatgacgcgg acttcgacct gagttggcac cttcgaaggt cttccgtgcc accaccaggt      300

gggtggaaag gcgtcctgga ttttgcccgc atgagcggga tggcagcctt cgacaaagat      360

cgtccgttat gggagctcac cgtgctcgat gggctaggcg gcggtgcaac agcgatggta      420

gtcaaagttc atcattcctt gaccgacggg gtcgggggta tgcaactgac acgcgaaatt      480

gccgatgaca cgcgggctgg cggatcaagt caggacggcc acgaattcag gccacctgtt      540

gttcgttcca caccaggaga ctcacgtgcg gcgatggccg aagcggtggc ccgacgtgcc      600

gcgcacgcag tgcgccatcc cgcagctacg atgcacgacg tcgtgcgaat cttcggatcg      660

acggcgcgta tgacgcgacc ggcgacccga acgatgtcgc cagtgatgac tgctcggagt      720

acgcgccgtg gtttcggtgt gatcgaattt ccggtggcgg cactgaccgc tgcagcggcg      780

ggcacacaat gctcggtaaa cgacgcgttc ttggctgcac ttctactggg catggccggc      840

taccacagag gccacggtga cgtaccgaat caactgatgg tgacattgcc gatcagtctg      900

cgtgacagtc gtgatccgct gggaggcaac cgaatcacgc ttgcaaggtt tgctcttccg      960

gtcgatattg cagatccgga gaaattgatc cgtcgtgttc acgtcatcgt agaaagttgg     1020

cggaacgagc cggcgattcc gctatctcct cacttggcca gaatgcttaa cttgctgccg     1080

gtcgcggtca caggcgacat gctcaagcac atggattttg ttgcatccaa cgtcgttggt     1140

tcttcgacac cgttgtacct cgccggtgcg cggataattc gccaattcgc attcagtcct     1200

accctcggat cggctttcaa cgcgactttg atgtcttacg cctcgcacgt gtgtgtcggg     1260

atcaacgtcg acgtgagagc agttccggac caacccgcat tgatgacagc cgtctccgaa     1320

gggtttagag ccgtgctggc acttgggact cggcacacag acgccacggt gacaacgtcg     1380

tactga                                                                1386


<210>  56
<211>  461
<212>  PRT
<213>  Rhodococcus opacus

<400>  56

Met Asn Arg Val Ala Met Ser Gly Ala Thr Glu Arg Ser Met Thr Gln 
1               5                   10                  15      


Thr Asp Leu Met Ser Trp Arg Met Glu Ala Asp Pro Val Leu Arg Ser 
            20                  25                  30          


Thr Ile Val Ser Ile Ile Val Leu Asp Arg Leu Pro Asp His Glu Arg 
        35                  40                  45              


Leu Thr Ala Ala Met Asn His Ala Ile Asp Val Val Pro Ile Phe Arg 
    50                  55                  60                  


Cys Lys Ala Val Ala Arg Gln Phe Pro Trp Ser Pro Pro Leu Trp Val 
65                  70                  75                  80  


Asp Asp Ala Asp Phe Asp Leu Ser Trp His Leu Arg Arg Ser Ser Val 
                85                  90                  95      


Pro Pro Pro Gly Gly Trp Lys Gly Val Leu Asp Phe Ala Arg Met Ser 
            100                 105                 110         


Gly Met Ala Ala Phe Asp Lys Asp Arg Pro Leu Trp Glu Leu Thr Val 
        115                 120                 125             


Leu Asp Gly Leu Gly Gly Gly Ala Thr Ala Met Val Val Lys Val His 
    130                 135                 140                 


His Ser Leu Thr Asp Gly Val Gly Gly Met Gln Leu Thr Arg Glu Ile 
145                 150                 155                 160 


Ala Asp Asp Thr Arg Ala Gly Gly Ser Ser Gln Asp Gly His Glu Phe 
                165                 170                 175     


Arg Pro Pro Val Val Arg Ser Thr Pro Gly Asp Ser Arg Ala Ala Met 
            180                 185                 190         


Ala Glu Ala Val Ala Arg Arg Ala Ala His Ala Val Arg His Pro Ala 
        195                 200                 205             


Ala Thr Met His Asp Val Val Arg Ile Phe Gly Ser Thr Ala Arg Met 
    210                 215                 220                 


Thr Arg Pro Ala Thr Arg Thr Met Ser Pro Val Met Thr Ala Arg Ser 
225                 230                 235                 240 


Thr Arg Arg Gly Phe Gly Val Ile Glu Phe Pro Val Ala Ala Leu Thr 
                245                 250                 255     


Ala Ala Ala Ala Gly Thr Gln Cys Ser Val Asn Asp Ala Phe Leu Ala 
            260                 265                 270         


Ala Leu Leu Leu Gly Met Ala Gly Tyr His Arg Gly His Gly Asp Val 
        275                 280                 285             


Pro Asn Gln Leu Met Val Thr Leu Pro Ile Ser Leu Arg Asp Ser Arg 
    290                 295                 300                 


Asp Pro Leu Gly Gly Asn Arg Ile Thr Leu Ala Arg Phe Ala Leu Pro 
305                 310                 315                 320 


Val Asp Ile Ala Asp Pro Glu Lys Leu Ile Arg Arg Val His Val Ile 
                325                 330                 335     


Val Glu Ser Trp Arg Asn Glu Pro Ala Ile Pro Leu Ser Pro His Leu 
            340                 345                 350         


Ala Arg Met Leu Asn Leu Leu Pro Val Ala Val Thr Gly Asp Met Leu 
        355                 360                 365             


Lys His Met Asp Phe Val Ala Ser Asn Val Val Gly Ser Ser Thr Pro 
    370                 375                 380                 


Leu Tyr Leu Ala Gly Ala Arg Ile Ile Arg Gln Phe Ala Phe Ser Pro 
385                 390                 395                 400 


Thr Leu Gly Ser Ala Phe Asn Ala Thr Leu Met Ser Tyr Ala Ser His 
                405                 410                 415     


Val Cys Val Gly Ile Asn Val Asp Val Arg Ala Val Pro Asp Gln Pro 
            420                 425                 430         


Ala Leu Met Thr Ala Val Ser Glu Gly Phe Arg Ala Val Leu Ala Leu 
        435                 440                 445             


Gly Thr Arg His Thr Asp Ala Thr Val Thr Thr Ser Tyr 
    450                 455                 460     


<210>  57
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer DGAT10_GLR001_PciI_F

<400>  57
agcggccgca agcttgccgc caaatggaag atgaggaccg cttg                        44


<210>  58
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer DGAT10_GLR001_PciI_R

<400>  58
gaccgacgta cctctgagtg acatgttact acttaataag gagtttcttg tcagg            55


<210>  59
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer DGAT10_416TEF_F

<400>  59
tctaatctaa gttttctaga actagtggat cccccgccac catggaagat gaggaccgct       60

tg                                                                      62


<210>  60
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EH_DGAT10 _416TEF_R

<400>  60
gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccttact actacttaat       60

aaggagtttc ttgtcagg                                                     78


<210>  61
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer DGAT2 _GLR001_PciI_F

<400>  61
agcggccgca agcttgccgc caaatggctg ccttctttcg gcgtaggaat g                51


<210>  62
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer DGAT2 _GLR001_PciI_R

<400>  62
gaccgacgta cctctgagtg acatgctacg aaatcgccac gaactcttcc gggcgtg          57


<210>  63
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer DGAT7__GLR001_PciI_F

<400>  63
agcggccgca agcttgccgc caaatggtgc ttggggaggg caaaaaaac                   49


<210>  64
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer DGAT7__GLR001_PciI_R

<400>  64
gaccgacgta cctctgagtg acatgctaaa acagatgcag ctcccggtc                   49


<210>  65
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M002

<400>  65
cgactctaga agatctttat tcattagtac tttcctgatt                             40


<210>  66
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M004

<400>  66
tcttgatgag catccatcat ccacggcagc ggcagca                                37


<210>  67
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M005

<400>  67
tcttgatgag catccatcat gatcccaaaa agccacaaag                             40


<210>  68
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M006

<400>  68
tgctgccgct gccgtggatg atggatgctc atcaagaa                               38


<210>  69
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M007

<400>  69
ctttgtggct ttttgggatc atgatggatg ctcatcaaga                             40


<210>  70
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M008

<400>  70
cgactctaga agatcttcaa tttccacaca caaacc                                 36


<210>  71
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M009

<400>  71
gcttgccgcc aacatggagg tttcaggcgc cgt                                    33


<210>  72
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer M0010

<400>  72
gcttgccgcc aacatgagac tattggtact tggtct                                 36


<210>  73
<211>  402
<212>  DNA
<213>  Nannochloropsis gaditana

<400>  73
atgagactat tggtacttgg tctcgtgtcc ctcggggtca cgggctttgc ccacacgcac       60

cctcgtctgt tgtccaagat tccgggagcc agtctcaaag gacgcccctg tttcgcggag      120

cccccctctg gtcctacctc ccgctccttc tctctgcctc ggagccgaac cgcggctctt      180

acatccgtag agccgagtgt gtataatttg caacctaccc cggttgtagc gccgaaactt      240

ttggtcagca gacgcggggg agcagttgcg acagctactg gatcagatgc tggagtcaag      300

cagaccttga aagtgggctc tttcttcttt ctttggtatt tgttcaacat cggctacaat      360

atctataaca agaaagccct caatgtgctg ccgctgccgt gg                         402


<210>  74
<211>  134
<212>  PRT
<213>  Nannochloropsis gaditana

<400>  74

Met Arg Leu Leu Val Leu Gly Leu Val Ser Leu Gly Val Thr Gly Phe 
1               5                   10                  15      


Ala His Thr His Pro Arg Leu Leu Ser Lys Ile Pro Gly Ala Ser Leu 
            20                  25                  30          


Lys Gly Arg Pro Cys Phe Ala Glu Pro Pro Ser Gly Pro Thr Ser Arg 
        35                  40                  45              


Ser Phe Ser Leu Pro Arg Ser Arg Thr Ala Ala Leu Thr Ser Val Glu 
    50                  55                  60                  


Pro Ser Val Tyr Asn Leu Gln Pro Thr Pro Val Val Ala Pro Lys Leu 
65                  70                  75                  80  


Leu Val Ser Arg Arg Gly Gly Ala Val Ala Thr Ala Thr Gly Ser Asp 
                85                  90                  95      


Ala Gly Val Lys Gln Thr Leu Lys Val Gly Ser Phe Phe Phe Leu Trp 
            100                 105                 110         


Tyr Leu Phe Asn Ile Gly Tyr Asn Ile Tyr Asn Lys Lys Ala Leu Asn 
        115                 120                 125             


Val Leu Pro Leu Pro Trp 
    130                 


<210>  75
<211>  468
<212>  DNA
<213>  Nannochloropsis gaditana

<400>  75
atgagactat tggtacttgg tctcgtgtcc ctcggggtca cgggctttgc ccacacgcac       60

cctcgtctgt tgtccaagat tccgggagcc agtctcaaag gacgcccctg tttcgcggag      120

cccccctctg gtcctacctc ccgctccttc tctctgcctc ggagccgaac cgcggctctt      180

acatccgtag agccgagtgt gtataatttg caacctaccc cggttgtagc gccgaaactt      240

ttggtcagca gacgcggggg agcagttgcg acagctactg gatcagatgc tggagtcaag      300

cagaccttga aagtgggctc tttcttcttt ctttggtatt tgttcaacat cggctacaat      360

atctataaca agaaagccct caatgtgctg ccgctgccgt ggatggtggg tttggtccag      420

ctctctctcg gcttgcttta tgtcttccct ttgtggcttt ttgggatc                   468


<210>  76
<211>  156
<212>  PRT
<213>  Nannochloropsis gaditana

<400>  76

Met Arg Leu Leu Val Leu Gly Leu Val Ser Leu Gly Val Thr Gly Phe 
1               5                   10                  15      


Ala His Thr His Pro Arg Leu Leu Ser Lys Ile Pro Gly Ala Ser Leu 
            20                  25                  30          


Lys Gly Arg Pro Cys Phe Ala Glu Pro Pro Ser Gly Pro Thr Ser Arg 
        35                  40                  45              


Ser Phe Ser Leu Pro Arg Ser Arg Thr Ala Ala Leu Thr Ser Val Glu 
    50                  55                  60                  


Pro Ser Val Tyr Asn Leu Gln Pro Thr Pro Val Val Ala Pro Lys Leu 
65                  70                  75                  80  


Leu Val Ser Arg Arg Gly Gly Ala Val Ala Thr Ala Thr Gly Ser Asp 
                85                  90                  95      


Ala Gly Val Lys Gln Thr Leu Lys Val Gly Ser Phe Phe Phe Leu Trp 
            100                 105                 110         


Tyr Leu Phe Asn Ile Gly Tyr Asn Ile Tyr Asn Lys Lys Ala Leu Asn 
        115                 120                 125             


Val Leu Pro Leu Pro Trp Met Val Gly Leu Val Gln Leu Ser Leu Gly 
    130                 135                 140                 


Leu Leu Tyr Val Phe Pro Leu Trp Leu Phe Gly Ile 
145                 150                 155     


<210>  77
<211>  1092
<212>  DNA
<213>  Euonymus alatus

<400>  77
atgatggatg ctcatcaaga gatcaagaac ttcatcaagg tttgggtaca agccatggta       60

tgtctatctt acgcttacta cttctcctca agacttccaa aaggactctt aaggttactc      120

tctttacttc ctgtccttta tctcctatta atcgctccct tgaacatctc ttctttcatt      180

ctttcatcaa tcaccggctt cttccttgct tggctaacaa cattcaaggt catctctttt      240

gcctttgatc aaggcccttt gtatccactc ccacaaaacc tcctacattt catctcaata      300

gcttgtcttc ccatcacaat caagcgaaac ccatcaccaa aattgaaatc cacaacaaac      360

ccatctccaa tttcacatct tttgaaaaag gcattcatgt catttccatc taaagtcttg      420

ttccattggg tcattgctca tctctaccaa tacaaaaagt acatggatcc aaacgtggtc      480

ttagtgatct attgttgtca tgtgtacgtt atgttggata tctcactctc tctatgtgca      540

accctagctg aattcctttg tgggtttgat gttgaaccac aattcaaaga gccttacctt      600

gctacttcac tacaagactt ttggggccgt aggtggaaca taattgtgtc aagtgtccta      660

cgttcgactg tctatgcccc aactagaaac attgcttcat atctaattgg gtctagatgg      720

gcttattttc cagctataat tgcaacattt gtcgtatcag gagttatgca tgatgtagtg      780

tactatgtgt acatgatgca tatgtatccc aagtgggata tgacagggca cttcgtccta      840

catgggattt gtgaagcttt ggaggtggag atgaagtgta agagatcaag gagtgacaag      900

tggcggcggc atccagctgt cgattgggta atggtgatgg ggtttgtgat ggggactagt      960

gtttccttac tctttgtgcc actattaagg gataatgtgg accaaattgt agcagaagag     1020

tactcaattt tgtttaattt tgtgagggag aagattgtca tgcttggaac aaggtttgtg     1080

tgtggaaatt ga                                                         1092


<210>  78
<211>  363
<212>  PRT
<213>  Euonymus alatus

<400>  78

Met Met Asp Ala His Gln Glu Ile Lys Asn Phe Ile Lys Val Trp Val 
1               5                   10                  15      


Gln Ala Met Val Cys Leu Ser Tyr Ala Tyr Tyr Phe Ser Ser Arg Leu 
            20                  25                  30          


Pro Lys Gly Leu Leu Arg Leu Leu Ser Leu Leu Pro Val Leu Tyr Leu 
        35                  40                  45              


Leu Leu Ile Ala Pro Leu Asn Ile Ser Ser Phe Ile Leu Ser Ser Ile 
    50                  55                  60                  


Thr Gly Phe Phe Leu Ala Trp Leu Thr Thr Phe Lys Val Ile Ser Phe 
65                  70                  75                  80  


Ala Phe Asp Gln Gly Pro Leu Tyr Pro Leu Pro Gln Asn Leu Leu His 
                85                  90                  95      


Phe Ile Ser Ile Ala Cys Leu Pro Ile Thr Ile Lys Arg Asn Pro Ser 
            100                 105                 110         


Pro Lys Leu Lys Ser Thr Thr Asn Pro Ser Pro Ile Ser His Leu Leu 
        115                 120                 125             


Lys Lys Ala Phe Met Ser Phe Pro Ser Lys Val Leu Phe His Trp Val 
    130                 135                 140                 


Ile Ala His Leu Tyr Gln Tyr Lys Lys Tyr Met Asp Pro Asn Val Val 
145                 150                 155                 160 


Leu Val Ile Tyr Cys Cys His Val Tyr Val Met Leu Asp Ile Ser Leu 
                165                 170                 175     


Ser Leu Cys Ala Thr Leu Ala Glu Phe Leu Cys Gly Phe Asp Val Glu 
            180                 185                 190         


Pro Gln Phe Lys Glu Pro Tyr Leu Ala Thr Ser Leu Gln Asp Phe Trp 
        195                 200                 205             


Gly Arg Arg Trp Asn Ile Ile Val Ser Ser Val Leu Arg Ser Thr Val 
    210                 215                 220                 


Tyr Ala Pro Thr Arg Asn Ile Ala Ser Tyr Leu Ile Gly Ser Arg Trp 
225                 230                 235                 240 


Ala Tyr Phe Pro Ala Ile Ile Ala Thr Phe Val Val Ser Gly Val Met 
                245                 250                 255     


His Asp Val Val Tyr Tyr Val Tyr Met Met His Met Tyr Pro Lys Trp 
            260                 265                 270         


Asp Met Thr Gly His Phe Val Leu His Gly Ile Cys Glu Ala Leu Glu 
        275                 280                 285             


Val Glu Met Lys Cys Lys Arg Ser Arg Ser Asp Lys Trp Arg Arg His 
    290                 295                 300                 


Pro Ala Val Asp Trp Val Met Val Met Gly Phe Val Met Gly Thr Ser 
305                 310                 315                 320 


Val Ser Leu Leu Phe Val Pro Leu Leu Arg Asp Asn Val Asp Gln Ile 
                325                 330                 335     


Val Ala Glu Glu Tyr Ser Ile Leu Phe Asn Phe Val Arg Glu Lys Ile 
            340                 345                 350         


Val Met Leu Gly Thr Arg Phe Val Cys Gly Asn 
        355                 360             


<210>  79
<211>  1637
<212>  DNA
<213>  Arachis hypogaea

<400>  79
aatgaacttg acataaagtg gttgtttgta acaccccatt tagtgttttg cttagatgtt       60

gagagttcta taaacttttg tactatttgg taccccgtaa ttaatagaaa tagaaatgtg      120

ataatggttc tatgtttcat tccagaaaaa aattgtcatt ttaaaaagtt ttcttaaatt      180

ctgaatggga atgatgatca ttcagatcaa taaggttaac acttttttat atgatatttt      240

atgtaatctg attaattttt ttttggtgac aaaaaactcg tgccgaattc ggcacgaggt      300

caaaacctca gaagagagaa aaggagaatt tggttcctaa ttaattctca ccatcaacga      360

tggaggtttc aggcgccgtt ctaaggaatg tcacgtgccc ttccttttct gtgcacgtga      420

gttcccgtcg tcgtggtggt gatagttgtg ttacagtgcc ggtgaggatg agaaaaaagg      480

cggtggtgcg ttgttgctgc gggttcagtg attcggggca tgtgcagtat tacggggacg      540

agaagaagaa ggagaatgga accgctatgt tgagcaccaa gaagaagctc aagatgctga      600

agaaacgtgt ccttttcgat gatcttcaag gaaacctgac ttgggatgct gctatggttt      660

tgatgaagca gctagagcaa gtaagggcag aggagaagga attgaagaaa aaaaggaagc      720

aagagaagaa ggaggcaaaa ctcaaagcct ctaagatgaa caccaatcct gattgcgaat      780

cgtcatcgtc atcgtcatca tctgaatctg aatctgaatc aagtgagagt gaatgtgaca      840

atgaggtggt tgacatgaag aagaacatta aggttggtgt tgccgttgct gttgccgatt      900

ccccacgaaa ggcggaaacc atgattctat acacctccct tgttgcccga gatgttagtg      960

ctaatcatca tcatcataat gccgtggaat tattctctag aaacaatgac atatcagttg     1020

gaagcattaa tggtggcctt aagaatgaga atactgcggt tattaccact gaagctattc     1080

ctcagaagag gattgaggta tgcatgggaa acaagtgcaa gaaatccgga tctattgcat     1140

tgttgcaaga atttgagaga gtggttggtg ctgaaggagg tgctgctgct gcagttgttg     1200

gatgcaagtg catggggaag tgcaagagtg cacctaatgt gaggattcag aactctactg     1260

cagataaaat agctgagggg ttcaatgatt cagttaaggt tccagctaac cctctttgca     1320

ttggggttgc atggaggatg ttgaaaccat tgtggcttag attcttgggc gagaatcagg     1380

aaagtactaa tgaataattt gctggtatgc tgtttggaaa attgtatata cgtagtgcca     1440

gaacctatca gattgttgtt ttattttata taaacataga ctgcatattg ttgtgagatt     1500

cgatttcctc atttattgga acttccagag cctgatttgt gtccattcga gctcgactca     1560

aagatttaca tggcctgctc aatctatgaa ttcaaatttg agggccctgt ttggcattaa     1620

tattaatata ttaatat                                                    1637


<210>  80
<211>  345
<212>  PRT
<213>  Arachis hypogaea

<400>  80

Met Glu Val Ser Gly Ala Val Leu Arg Asn Val Thr Cys Pro Ser Phe 
1               5                   10                  15      


Ser Val His Val Ser Ser Arg Arg Arg Gly Gly Asp Ser Cys Val Thr 
            20                  25                  30          


Val Pro Val Arg Met Arg Lys Lys Ala Val Val Arg Cys Cys Cys Gly 
        35                  40                  45              


Phe Ser Asp Ser Gly His Val Gln Tyr Tyr Gly Asp Glu Lys Lys Lys 
    50                  55                  60                  


Glu Asn Gly Thr Ala Met Leu Ser Thr Lys Lys Lys Leu Lys Met Leu 
65                  70                  75                  80  


Lys Lys Arg Val Leu Phe Asp Asp Leu Gln Gly Asn Leu Thr Trp Asp 
                85                  90                  95      


Ala Ala Met Val Leu Met Lys Gln Leu Glu Gln Val Arg Ala Glu Glu 
            100                 105                 110         


Lys Glu Leu Lys Lys Lys Arg Lys Gln Glu Lys Lys Glu Ala Lys Leu 
        115                 120                 125             


Lys Ala Ser Lys Met Asn Thr Asn Pro Asp Cys Glu Ser Ser Ser Ser 
    130                 135                 140                 


Ser Ser Ser Ser Glu Ser Glu Ser Glu Ser Ser Glu Ser Glu Cys Asp 
145                 150                 155                 160 


Asn Glu Val Val Asp Met Lys Lys Asn Ile Lys Val Gly Val Ala Val 
                165                 170                 175     


Ala Val Ala Asp Ser Pro Arg Lys Ala Glu Thr Met Ile Leu Tyr Thr 
            180                 185                 190         


Ser Leu Val Ala Arg Asp Val Ser Ala Asn His His His His Asn Ala 
        195                 200                 205             


Val Glu Leu Phe Ser Arg Asn Asn Asp Ile Ser Val Gly Ser Ile Asn 
    210                 215                 220                 


Gly Gly Leu Lys Asn Glu Asn Thr Ala Val Ile Thr Thr Glu Ala Ile 
225                 230                 235                 240 


Pro Gln Lys Arg Ile Glu Val Cys Met Gly Asn Lys Cys Lys Lys Ser 
                245                 250                 255     


Gly Ser Ile Ala Leu Leu Gln Glu Phe Glu Arg Val Val Gly Ala Glu 
            260                 265                 270         


Gly Gly Ala Ala Ala Ala Val Val Gly Cys Lys Cys Met Gly Lys Cys 
        275                 280                 285             


Lys Ser Ala Pro Asn Val Arg Ile Gln Asn Ser Thr Ala Asp Lys Ile 
    290                 295                 300                 


Ala Glu Gly Phe Asn Asp Ser Val Lys Val Pro Ala Asn Pro Leu Cys 
305                 310                 315                 320 


Ile Gly Val Ala Trp Arg Met Leu Lys Pro Leu Trp Leu Arg Phe Leu 
                325                 330                 335     


Gly Glu Asn Gln Glu Ser Thr Asn Glu 
            340                 345 


<210>  81
<211>  1494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Euonymus alatus DGAT fused at N-terminus to G6P/Pi-Tporter 
       targeting signal and 1TM domain (EaDGAT/1TM)

<400>  81
atgagactat tggtacttgg tctcgtgtcc ctcggggtca cgggctttgc ccacacgcac       60

cctcgtctgt tgtccaagat tccgggagcc agtctcaaag gacgcccctg tttcgcggag      120

cccccctctg gtcctacctc ccgctccttc tctctgcctc ggagccgaac cgcggctctt      180

acatccgtag agccgagtgt gtataatttg caacctaccc cggttgtagc gccgaaactt      240

ttggtcagca gacgcggggg agcagttgcg acagctactg gatcagatgc tggagtcaag      300

cagaccttga aagtgggctc tttcttcttt ctttggtatt tgttcaacat cggctacaat      360

atctataaca agaaagccct caatgtgctg ccgctgccgt ggatgatgga tgctcatcaa      420

gagatcaaga acttcatcaa ggtttgggta caagccatgg tatgtctatc ttacgcttac      480

tacttctcct caagacttcc aaaaggactc ttaaggttac tctctttact tcctgtcctt      540

tatctcctat taatcgctcc cttgaacatc tcttctttca ttctttcatc aatcaccggc      600

ttcttccttg cttggctaac aacattcaag gtcatctctt ttgcctttga tcaaggccct      660

ttgtatccac tcccacaaaa cctcctacat ttcatctcaa tagcttgtct tcccatcaca      720

atcaagcgaa acccatcacc aaaattgaaa tccacaacaa acccatctcc aatttcacat      780

cttttgaaaa aggcattcat gtcatttcca tctaaagtct tgttccattg ggtcattgct      840

catctctacc aatacaaaaa gtacatggat ccaaacgtgg tcttagtgat ctattgttgt      900

catgtgtacg ttatgttgga tatctcactc tctctatgtg caaccctagc tgaattcctt      960

tgtgggtttg atgttgaacc acaattcaaa gagccttacc ttgctacttc actacaagac     1020

ttttggggcc gtaggtggaa cataattgtg tcaagtgtcc tacgttcgac tgtctatgcc     1080

ccaactagaa acattgcttc atatctaatt gggtctagat gggcttattt tccagctata     1140

attgcaacat ttgtcgtatc aggagttatg catgatgtag tgtactatgt gtacatgatg     1200

catatgtatc ccaagtggga tatgacaggg cacttcgtcc tacatgggat ttgtgaagct     1260

ttggaggtgg agatgaagtg taagagatca aggagtgaca agtggcggcg gcatccagct     1320

gtcgattggg taatggtgat ggggtttgtg atggggacta gtgtttcctt actctttgtg     1380

ccactattaa gggataatgt ggaccaaatt gtagcagaag agtactcaat tttgtttaat     1440

tttgtgaggg agaagattgt catgcttgga acaaggtttg tgtgtggaaa ttga           1494


<210>  82
<211>  497
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Euonymus alatus DGAT fused at N-terminus to G6P/Pi-Tporter 
       targeting signal and 1TM domain (EaDGAT/1TM)

<400>  82

Met Arg Leu Leu Val Leu Gly Leu Val Ser Leu Gly Val Thr Gly Phe 
1               5                   10                  15      


Ala His Thr His Pro Arg Leu Leu Ser Lys Ile Pro Gly Ala Ser Leu 
            20                  25                  30          


Lys Gly Arg Pro Cys Phe Ala Glu Pro Pro Ser Gly Pro Thr Ser Arg 
        35                  40                  45              


Ser Phe Ser Leu Pro Arg Ser Arg Thr Ala Ala Leu Thr Ser Val Glu 
    50                  55                  60                  


Pro Ser Val Tyr Asn Leu Gln Pro Thr Pro Val Val Ala Pro Lys Leu 
65                  70                  75                  80  


Leu Val Ser Arg Arg Gly Gly Ala Val Ala Thr Ala Thr Gly Ser Asp 
                85                  90                  95      


Ala Gly Val Lys Gln Thr Leu Lys Val Gly Ser Phe Phe Phe Leu Trp 
            100                 105                 110         


Tyr Leu Phe Asn Ile Gly Tyr Asn Ile Tyr Asn Lys Lys Ala Leu Asn 
        115                 120                 125             


Val Leu Pro Leu Pro Trp Met Met Asp Ala His Gln Glu Ile Lys Asn 
    130                 135                 140                 


Phe Ile Lys Val Trp Val Gln Ala Met Val Cys Leu Ser Tyr Ala Tyr 
145                 150                 155                 160 


Tyr Phe Ser Ser Arg Leu Pro Lys Gly Leu Leu Arg Leu Leu Ser Leu 
                165                 170                 175     


Leu Pro Val Leu Tyr Leu Leu Leu Ile Ala Pro Leu Asn Ile Ser Ser 
            180                 185                 190         


Phe Ile Leu Ser Ser Ile Thr Gly Phe Phe Leu Ala Trp Leu Thr Thr 
        195                 200                 205             


Phe Lys Val Ile Ser Phe Ala Phe Asp Gln Gly Pro Leu Tyr Pro Leu 
    210                 215                 220                 


Pro Gln Asn Leu Leu His Phe Ile Ser Ile Ala Cys Leu Pro Ile Thr 
225                 230                 235                 240 


Ile Lys Arg Asn Pro Ser Pro Lys Leu Lys Ser Thr Thr Asn Pro Ser 
                245                 250                 255     


Pro Ile Ser His Leu Leu Lys Lys Ala Phe Met Ser Phe Pro Ser Lys 
            260                 265                 270         


Val Leu Phe His Trp Val Ile Ala His Leu Tyr Gln Tyr Lys Lys Tyr 
        275                 280                 285             


Met Asp Pro Asn Val Val Leu Val Ile Tyr Cys Cys His Val Tyr Val 
    290                 295                 300                 


Met Leu Asp Ile Ser Leu Ser Leu Cys Ala Thr Leu Ala Glu Phe Leu 
305                 310                 315                 320 


Cys Gly Phe Asp Val Glu Pro Gln Phe Lys Glu Pro Tyr Leu Ala Thr 
                325                 330                 335     


Ser Leu Gln Asp Phe Trp Gly Arg Arg Trp Asn Ile Ile Val Ser Ser 
            340                 345                 350         


Val Leu Arg Ser Thr Val Tyr Ala Pro Thr Arg Asn Ile Ala Ser Tyr 
        355                 360                 365             


Leu Ile Gly Ser Arg Trp Ala Tyr Phe Pro Ala Ile Ile Ala Thr Phe 
    370                 375                 380                 


Val Val Ser Gly Val Met His Asp Val Val Tyr Tyr Val Tyr Met Met 
385                 390                 395                 400 


His Met Tyr Pro Lys Trp Asp Met Thr Gly His Phe Val Leu His Gly 
                405                 410                 415     


Ile Cys Glu Ala Leu Glu Val Glu Met Lys Cys Lys Arg Ser Arg Ser 
            420                 425                 430         


Asp Lys Trp Arg Arg His Pro Ala Val Asp Trp Val Met Val Met Gly 
        435                 440                 445             


Phe Val Met Gly Thr Ser Val Ser Leu Leu Phe Val Pro Leu Leu Arg 
    450                 455                 460                 


Asp Asn Val Asp Gln Ile Val Ala Glu Glu Tyr Ser Ile Leu Phe Asn 
465                 470                 475                 480 


Phe Val Arg Glu Lys Ile Val Met Leu Gly Thr Arg Phe Val Cys Gly 
                485                 490                 495     


Asn 
    


<210>  83
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Euonymus alatus DGAT fused at N-terminus to G6P/Pi-Tporter 
       targeting signal and 2TM domain (EaDGAT/2TM)

<400>  83
atgagactat tggtacttgg tctcgtgtcc ctcggggtca cgggctttgc ccacacgcac       60

cctcgtctgt tgtccaagat tccgggagcc agtctcaaag gacgcccctg tttcgcggag      120

cccccctctg gtcctacctc ccgctccttc tctctgcctc ggagccgaac cgcggctctt      180

acatccgtag agccgagtgt gtataatttg caacctaccc cggttgtagc gccgaaactt      240

ttggtcagca gacgcggggg agcagttgcg acagctactg gatcagatgc tggagtcaag      300

cagaccttga aagtgggctc tttcttcttt ctttggtatt tgttcaacat cggctacaat      360

atctataaca agaaagccct caatgtgctg ccgctgccgt ggatggtggg tttggtccag      420

ctctctctcg gcttgcttta tgtcttccct ttgtggcttt ttgggatcat gatggatgct      480

catcaagaga tcaagaactt catcaaggtt tgggtacaag ccatggtatg tctatcttac      540

gcttactact tctcctcaag acttccaaaa ggactcttaa ggttactctc tttacttcct      600

gtcctttatc tcctattaat cgctcccttg aacatctctt ctttcattct ttcatcaatc      660

accggcttct tccttgcttg gctaacaaca ttcaaggtca tctcttttgc ctttgatcaa      720

ggccctttgt atccactccc acaaaacctc ctacatttca tctcaatagc ttgtcttccc      780

atcacaatca agcgaaaccc atcaccaaaa ttgaaatcca caacaaaccc atctccaatt      840

tcacatcttt tgaaaaaggc attcatgtca tttccatcta aagtcttgtt ccattgggtc      900

attgctcatc tctaccaata caaaaagtac atggatccaa acgtggtctt agtgatctat      960

tgttgtcatg tgtacgttat gttggatatc tcactctctc tatgtgcaac cctagctgaa     1020

ttcctttgtg ggtttgatgt tgaaccacaa ttcaaagagc cttaccttgc tacttcacta     1080

caagactttt ggggccgtag gtggaacata attgtgtcaa gtgtcctacg ttcgactgtc     1140

tatgccccaa ctagaaacat tgcttcatat ctaattgggt ctagatgggc ttattttcca     1200

gctataattg caacatttgt cgtatcagga gttatgcatg atgtagtgta ctatgtgtac     1260

atgatgcata tgtatcccaa gtgggatatg acagggcact tcgtcctaca tgggatttgt     1320

gaagctttgg aggtggagat gaagtgtaag agatcaagga gtgacaagtg gcggcggcat     1380

ccagctgtcg attgggtaat ggtgatgggg tttgtgatgg ggactagtgt ttccttactc     1440

tttgtgccac tattaaggga taatgtggac caaattgtag cagaagagta ctcaattttg     1500

tttaattttg tgagggagaa gattgtcatg cttggaacaa ggtttgtgtg tggaaattga     1560


<210>  84
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Euonymus alatus DGAT fused at N-terminus to G6P/Pi-Tporter 
       targeting signal and 2TM domain (EaDGAT/2TM)

<400>  84

Met Arg Leu Leu Val Leu Gly Leu Val Ser Leu Gly Val Thr Gly Phe 
1               5                   10                  15      


Ala His Thr His Pro Arg Leu Leu Ser Lys Ile Pro Gly Ala Ser Leu 
            20                  25                  30          


Lys Gly Arg Pro Cys Phe Ala Glu Pro Pro Ser Gly Pro Thr Ser Arg 
        35                  40                  45              


Ser Phe Ser Leu Pro Arg Ser Arg Thr Ala Ala Leu Thr Ser Val Glu 
    50                  55                  60                  


Pro Ser Val Tyr Asn Leu Gln Pro Thr Pro Val Val Ala Pro Lys Leu 
65                  70                  75                  80  


Leu Val Ser Arg Arg Gly Gly Ala Val Ala Thr Ala Thr Gly Ser Asp 
                85                  90                  95      


Ala Gly Val Lys Gln Thr Leu Lys Val Gly Ser Phe Phe Phe Leu Trp 
            100                 105                 110         


Tyr Leu Phe Asn Ile Gly Tyr Asn Ile Tyr Asn Lys Lys Ala Leu Asn 
        115                 120                 125             


Val Leu Pro Leu Pro Trp Met Val Gly Leu Val Gln Leu Ser Leu Gly 
    130                 135                 140                 


Leu Leu Tyr Val Phe Pro Leu Trp Leu Phe Gly Ile Met Met Asp Ala 
145                 150                 155                 160 


His Gln Glu Ile Lys Asn Phe Ile Lys Val Trp Val Gln Ala Met Val 
                165                 170                 175     


Cys Leu Ser Tyr Ala Tyr Tyr Phe Ser Ser Arg Leu Pro Lys Gly Leu 
            180                 185                 190         


Leu Arg Leu Leu Ser Leu Leu Pro Val Leu Tyr Leu Leu Leu Ile Ala 
        195                 200                 205             


Pro Leu Asn Ile Ser Ser Phe Ile Leu Ser Ser Ile Thr Gly Phe Phe 
    210                 215                 220                 


Leu Ala Trp Leu Thr Thr Phe Lys Val Ile Ser Phe Ala Phe Asp Gln 
225                 230                 235                 240 


Gly Pro Leu Tyr Pro Leu Pro Gln Asn Leu Leu His Phe Ile Ser Ile 
                245                 250                 255     


Ala Cys Leu Pro Ile Thr Ile Lys Arg Asn Pro Ser Pro Lys Leu Lys 
            260                 265                 270         


Ser Thr Thr Asn Pro Ser Pro Ile Ser His Leu Leu Lys Lys Ala Phe 
        275                 280                 285             


Met Ser Phe Pro Ser Lys Val Leu Phe His Trp Val Ile Ala His Leu 
    290                 295                 300                 


Tyr Gln Tyr Lys Lys Tyr Met Asp Pro Asn Val Val Leu Val Ile Tyr 
305                 310                 315                 320 


Cys Cys His Val Tyr Val Met Leu Asp Ile Ser Leu Ser Leu Cys Ala 
                325                 330                 335     


Thr Leu Ala Glu Phe Leu Cys Gly Phe Asp Val Glu Pro Gln Phe Lys 
            340                 345                 350         


Glu Pro Tyr Leu Ala Thr Ser Leu Gln Asp Phe Trp Gly Arg Arg Trp 
        355                 360                 365             


Asn Ile Ile Val Ser Ser Val Leu Arg Ser Thr Val Tyr Ala Pro Thr 
    370                 375                 380                 


Arg Asn Ile Ala Ser Tyr Leu Ile Gly Ser Arg Trp Ala Tyr Phe Pro 
385                 390                 395                 400 


Ala Ile Ile Ala Thr Phe Val Val Ser Gly Val Met His Asp Val Val 
                405                 410                 415     


Tyr Tyr Val Tyr Met Met His Met Tyr Pro Lys Trp Asp Met Thr Gly 
            420                 425                 430         


His Phe Val Leu His Gly Ile Cys Glu Ala Leu Glu Val Glu Met Lys 
        435                 440                 445             


Cys Lys Arg Ser Arg Ser Asp Lys Trp Arg Arg His Pro Ala Val Asp 
    450                 455                 460                 


Trp Val Met Val Met Gly Phe Val Met Gly Thr Ser Val Ser Leu Leu 
465                 470                 475                 480 


Phe Val Pro Leu Leu Arg Asp Asn Val Asp Gln Ile Val Ala Glu Glu 
                485                 490                 495     


Tyr Ser Ile Leu Phe Asn Phe Val Arg Glu Lys Ile Val Met Leu Gly 
            500                 505                 510         


Thr Arg Phe Val Cys Gly Asn 
        515                 


<210>  85
<211>  1161
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ER targeting signal and Mus musculus DGAT2 truncated by 55 amino 
       acid residues

<400>  85
atgatgttca tgcgaatcgc cgtcgccgct ctggccttgc tggcagcgcc atcgatcagg       60

gccgaggagg ctggcgagga ggcgaaaatg ggcactgtgg gtggaggcag ctacccttat      120

gatgttcccg actacgctgg cggtgggtcg gtgacgtggc tcaaccgcag caaggtggag      180

aaacaattgc aagttatctc tgtccttcag tgggtcctca gctttctcgt gctcggggtg      240

gcctgctctg tcatcttgat gtatacgttc tgcacagatt gttggctcat cgcggtgttg      300

tacttcacct ggcttgcatt cgactggaac acccccaaaa agggtggtcg ccggtcgcag      360

tgggtgcgca attgggccgt gtggcggtac ttccgcgact acttccctat tcaattggtc      420

aaaacccata acttgctcac tacccggaac tacatctttg gctaccatcc gcacgggatc      480

atggggcttg gtgcgttctg caacttttcg acggaagcca cggaagtgtc caagaagttc      540

cccggcattc gtccttactt ggccaccctc gcgggcaact tccgtatgcc tgtgttgcga      600

gagtacctga tgtccggggg aatttgtccc gttaatcgtg acaccatcga ctaccttctc      660

tccaagaatg gctctggcaa cgcaatcatc attgtggtcg gcggagctgc tgagtccctc      720

tcctcgatgc cgggaaagaa tgcagttact ctcaagaatc gcaagggctt tgtgaagctg      780

gccctgcggc acggtgccga tctggtgccc acgtacagct ttggcgagaa cgaggtgtac      840

aagcaggtca tctttgaaga gggaagctgg ggcaggtggg tccagaaaaa gttccagaag      900

tacatcgggt tcgctccctg cattttccac gggcgtggcc tgttttcctc cgacacgtgg      960

gggctggtgc cttatagcaa gccgattacc accgtggtcg gagagccaat tacggtcccg     1020

aaactggagc atcccactca aaaggacatt gatctttatc acgcgatgta tatggaagcg     1080

ttggtgaagc tgtttgataa ccataagacc aagttcgggc ttcccgaaac agaagtgctg     1140

gaggtcaacg acgacgagtt g                                               1161


<210>  86
<211>  387
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ER targeting signal and Mus musculus DGAT2 truncated by 55 amino 
       acid residues

<400>  86

Met Met Phe Met Arg Ile Ala Val Ala Ala Leu Ala Leu Leu Ala Ala 
1               5                   10                  15      


Pro Ser Ile Arg Ala Glu Glu Ala Gly Glu Glu Ala Lys Met Gly Thr 
            20                  25                  30          


Val Gly Gly Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Gly 
        35                  40                  45              


Gly Ser Val Thr Trp Leu Asn Arg Ser Lys Val Glu Lys Gln Leu Gln 
    50                  55                  60                  


Val Ile Ser Val Leu Gln Trp Val Leu Ser Phe Leu Val Leu Gly Val 
65                  70                  75                  80  


Ala Cys Ser Val Ile Leu Met Tyr Thr Phe Cys Thr Asp Cys Trp Leu 
                85                  90                  95      


Ile Ala Val Leu Tyr Phe Thr Trp Leu Ala Phe Asp Trp Asn Thr Pro 
            100                 105                 110         


Lys Lys Gly Gly Arg Arg Ser Gln Trp Val Arg Asn Trp Ala Val Trp 
        115                 120                 125             


Arg Tyr Phe Arg Asp Tyr Phe Pro Ile Gln Leu Val Lys Thr His Asn 
    130                 135                 140                 


Leu Leu Thr Thr Arg Asn Tyr Ile Phe Gly Tyr His Pro His Gly Ile 
145                 150                 155                 160 


Met Gly Leu Gly Ala Phe Cys Asn Phe Ser Thr Glu Ala Thr Glu Val 
                165                 170                 175     


Ser Lys Lys Phe Pro Gly Ile Arg Pro Tyr Leu Ala Thr Leu Ala Gly 
            180                 185                 190         


Asn Phe Arg Met Pro Val Leu Arg Glu Tyr Leu Met Ser Gly Gly Ile 
        195                 200                 205             


Cys Pro Val Asn Arg Asp Thr Ile Asp Tyr Leu Leu Ser Lys Asn Gly 
    210                 215                 220                 


Ser Gly Asn Ala Ile Ile Ile Val Val Gly Gly Ala Ala Glu Ser Leu 
225                 230                 235                 240 


Ser Ser Met Pro Gly Lys Asn Ala Val Thr Leu Lys Asn Arg Lys Gly 
                245                 250                 255     


Phe Val Lys Leu Ala Leu Arg His Gly Ala Asp Leu Val Pro Thr Tyr 
            260                 265                 270         


Ser Phe Gly Glu Asn Glu Val Tyr Lys Gln Val Ile Phe Glu Glu Gly 
        275                 280                 285             


Ser Trp Gly Arg Trp Val Gln Lys Lys Phe Gln Lys Tyr Ile Gly Phe 
    290                 295                 300                 


Ala Pro Cys Ile Phe His Gly Arg Gly Leu Phe Ser Ser Asp Thr Trp 
305                 310                 315                 320 


Gly Leu Val Pro Tyr Ser Lys Pro Ile Thr Thr Val Val Gly Glu Pro 
                325                 330                 335     


Ile Thr Val Pro Lys Leu Glu His Pro Thr Gln Lys Asp Ile Asp Leu 
            340                 345                 350         


Tyr His Ala Met Tyr Met Glu Ala Leu Val Lys Leu Phe Asp Asn His 
        355                 360                 365             


Lys Thr Lys Phe Gly Leu Pro Glu Thr Glu Val Leu Glu Val Asn Asp 
    370                 375                 380                 


Asp Glu Leu 
385         


<210>  87
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Eukaryotic DGAT1 consensus motif


<220>
<221>  DOMAIN
<222>  (1)..(7)
<223>  X can be any amino acid residue

<400>  87

Phe Tyr Xaa Asp Trp Trp Asn 
1               5           


