                         SEQUENCE LISTING

<110>  Universiteit Gent
 
<120>  A thermostable sucrose phosphorylase

<130>  P2011/101PCT

<150>  EP12188966.1
<151>  2012-10-18

<150>  EP13159683.5
<151>  2013-03-18

<160>  5     

<170>  PatentIn version 3.3

<210>  1
<211>  488
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  1

Met Ala Leu Lys Asn Lys Val Gln Leu Ile Thr Tyr Pro Asp Ser Leu 
1               5                   10                  15      


Gly Gly Asn Leu Lys Thr Leu Asn Asp Val Leu Glu Lys Tyr Phe Ser 
            20                  25                  30          


Asp Val Phe Gly Gly Val His Ile Leu Pro Pro Phe Pro Ser Ser Gly 
        35                  40                  45              


Asp Arg Gly Phe Ala Pro Ile Thr Tyr Ser Glu Ile Glu Pro Lys Phe 
    50                  55                  60                  


Gly Thr Trp Tyr Asp Ile Lys Lys Met Ala Glu Asn Phe Asp Ile Leu 
65                  70                  75                  80  


Leu Asp Leu Met Val Asn His Val Ser Arg Arg Ser Ile Tyr Phe Gln 
                85                  90                  95      


Asp Phe Leu Lys Lys Gly Arg Lys Ser Glu Tyr Ala Asp Met Phe Ile 
            100                 105                 110         


Thr Leu Asp Lys Leu Trp Lys Asp Gly Lys Pro Val Lys Gly Asp Ile 
        115                 120                 125             


Glu Lys Met Phe Leu Arg Arg Thr Leu Pro Tyr Ser Thr Phe Lys Ile 
    130                 135                 140                 


Glu Glu Thr Gly Glu Glu Glu Lys Val Trp Thr Thr Phe Gly Lys Thr 
145                 150                 155                 160 


Asp Pro Ser Glu Gln Ile Asp Leu Asp Val Asn Ser His Leu Val Arg 
                165                 170                 175     


Glu Phe Leu Leu Glu Val Phe Lys Thr Phe Ser Asn Phe Gly Val Lys 
            180                 185                 190         


Ile Val Arg Leu Asp Ala Val Gly Tyr Val Ile Lys Lys Ile Gly Thr 
        195                 200                 205             


Ser Cys Phe Phe Val Glu Pro Glu Ile Tyr Glu Phe Leu Asp Trp Ala 
    210                 215                 220                 


Lys Gly Gln Ala Ala Ser Tyr Gly Ile Glu Leu Leu Leu Glu Val His 
225                 230                 235                 240 


Ser Gln Phe Glu Val Gln Tyr Lys Leu Ala Glu Arg Gly Phe Leu Ile 
                245                 250                 255     


Tyr Asp Phe Ile Leu Pro Phe Thr Val Leu Tyr Thr Leu Ile Asn Lys 
            260                 265                 270         


Ser Asn Glu Met Leu Tyr His Tyr Leu Lys Asn Arg Pro Ile Asn Gln 
        275                 280                 285             


Phe Thr Met Leu Asp Cys His Asp Gly Ile Pro Val Lys Pro Asp Leu 
    290                 295                 300                 


Asp Gly Leu Ile Asp Thr Lys Lys Ala Lys Glu Val Val Asp Ile Cys 
305                 310                 315                 320 


Val Gln Arg Gly Ala Asn Leu Ser Leu Ile Tyr Gly Asp Lys Tyr Lys 
                325                 330                 335     


Ser Glu Asp Gly Phe Asp Val His Gln Ile Asn Cys Thr Tyr Tyr Ser 
            340                 345                 350         


Ala Leu Asn Cys Asp Asp Asp Ala Tyr Leu Ala Ala Arg Ala Ile Gln 
        355                 360                 365             


Phe Phe Thr Pro Gly Ile Pro Gln Val Tyr Tyr Val Gly Leu Leu Ala 
    370                 375                 380                 


Gly Val Asn Asp Phe Glu Ala Val Lys Lys Thr Lys Glu Gly Arg Glu 
385                 390                 395                 400 


Ile Asn Arg His Asn Tyr Gly Leu Lys Glu Ile Glu Glu Ser Val Gln 
                405                 410                 415     


Lys Asn Val Val Gln Arg Leu Leu Lys Leu Ile Arg Phe Arg Asn Glu 
            420                 425                 430         


Tyr Glu Ala Phe Asn Gly Glu Phe Phe Ile Glu Asp Cys Arg Lys Asp 
        435                 440                 445             


Glu Ile Arg Leu Thr Trp Lys Lys Asp Asp Lys Arg Cys Ser Leu Phe 
    450                 455                 460                 


Ile Asp Leu Lys Thr Tyr Lys Thr Thr Ile Asp Tyr Ile Asn Glu Asn 
465                 470                 475                 480 


Gly Glu Glu Val Lys Tyr Leu Val 
                485             


<210>  2
<211>  1467
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  2
atggcactga aaaacaaagt ccaactgatt acctatccgg acagcctggg cggtaacctg     60

aaaaccctga atgacgttct ggaaaaatat ttcagcgatg tgtttggcgg tgttcatatc    120

ctgccgccgt tcccgagctc tggtgaccgt ggttttgcac cgattaccta ctctgaaatc    180

gaaccgaaat tcggcacgtg gtacgatatt aagaaaatgg ctgaaaactt cgacatcctg    240

ctggatctga tggttaatca cgtcagtcgt cgctccattt actttcagga cttcctgaaa    300

aaaggccgca aaagtgaata tgcggatatg tttattaccc tggacaaact gtggaaagat    360

ggcaaaccgg ttaaaggtga tatcgaaaaa atgttcctgc gtcgcaccct gccgtactcc    420

acgtttaaaa ttgaagaaac cggtgaagaa gaaaaagtct ggaccacgtt cggcaaaacg    480

gatccgtcag aacagatcga cctggatgtc aactcgcatc tggtgcgtga atttctgctg    540

gaagtgttca aaaccttctc aaacttcggt gtgaaaattg ttcgcctgga tgcggtcggc    600

tatgtgatta agaaaattgg cacgtcgtgc tttttcgttg aaccggaaat ctacgaattt    660

ctggattggg ccaaaggcca ggcggccagt tatggtattg aactgctgct ggaagttcac    720

tcccagttcg aagtccaata taaactggca gaacgtggct ttctgattta cgatttcatc    780

ctgccgttta ccgtgctgta tacgctgatc aacaaaagta acgaaatgct gtaccattac    840

ctgaaaaacc gcccgattaa tcaatttacc atgctggact gccacgatgg tattccggtc    900

aaaccggacc tggatggcct gatcgacacg aaaaaagcga aagaagtggt tgatatttgt    960

gtgcagcgtg gcgccaacct gagcctgatc tatggtgata aatacaaatc tgaagacggc   1020

ttcgatgttc atcaaattaa ctgcacctat tacagcgctc tgaattgtga tgacgatgcg   1080

tatctggcag ctcgcgccat tcagtttttc acgccgggta tcccgcaagt ttattacgtc   1140

ggcctgctgg caggtgtgaa cgattttgaa gctgtgaaga aaaccaaaga aggtcgtgaa   1200

attaaccgcc acaattacgg cctgaaagaa atcgaagaat ctgtgcagaa aaatgtcgtg   1260

caacgtctgc tgaaactgat ccgtttccgc aacgaatatg aagcctttaa tggcgaattt   1320

ttcattgaag actgccgtaa agatgaaatc cgcctgacct ggaaaaaaga cgataaacgc   1380

tgtagcctgt ttattgatct gaaaacctac aaaacgacga ttgattacat taacgaaaac   1440

ggtgaagaag tgaaatacct ggtttaa                                       1467


<210>  3
<211>  1509
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  3
atgggcggtt cgcaccacca ccaccaccac ggcatggcta gcatggcact gaaaaacaaa     60

gtccaactga ttacctatcc ggacagcctg ggcggtaacc tgaaaaccct gaatgacgtt    120

ctggaaaaat atttcagcga tgtgtttggc ggtgttcata tcctgccgcc gttcccgagc    180

tctggtgacc gtggttttgc accgattacc tactctgaaa tcgaaccgaa attcggcacg    240

tggtacgata ttaagaaaat ggctgaaaac ttcgacatcc tgctggatct gatggttaat    300

cacgtcagtc gtcgctccat ttactttcag gacttcctga aaaaaggccg caaaagtgaa    360

tatgcggata tgtttattac cctggacaaa ctgtggaaag atggcaaacc ggttaaaggt    420

gatatcgaaa aaatgttcct gcgtcgcacc ctgccgtact ccacgtttaa aattgaagaa    480

accggtgaag aagaaaaagt ctggaccacg ttcggcaaaa cggatccgtc agaacagatc    540

gacctggatg tcaactcgca tctggtgcgt gaatttctgc tggaagtgtt caaaaccttc    600

tcaaacttcg gtgtgaaaat tgttcgcctg gatgcggtcg gctatgtgat taagaaaatt    660

ggcacgtcgt gctttttcgt tgaaccggaa atctacgaat ttctggattg ggccaaaggc    720

caggcggcca gttatggtat tgaactgctg ctggaagttc actcccagtt cgaagtccaa    780

tataaactgg cagaacgtgg ctttctgatt tacgatttca tcctgccgtt taccgtgctg    840

tatacgctga tcaacaaaag taacgaaatg ctgtaccatt acctgaaaaa ccgcccgatt    900

aatcaattta ccatgctgga ctgccacgat ggtattccgg tcaaaccgga cctggatggc    960

ctgatcgaca cgaaaaaagc gaaagaagtg gttgatattt gtgtgcagcg tggcgccaac   1020

ctgagcctga tctatggtga taaatacaaa tctgaagacg gcttcgatgt tcatcaaatt   1080

aactgcacct attacagcgc tctgaattgt gatgacgatg cgtatctggc agctcgcgcc   1140

attcagtttt tcacgccggg tatcccgcaa gtttattacg tcggcctgct ggcaggtgtg   1200

aacgattttg aagctgtgaa gaaaaccaaa gaaggtcgtg aaattaaccg ccacaattac   1260

ggcctgaaag aaatcgaaga atctgtgcag aaaaatgtcg tgcaacgtct gctgaaactg   1320

atccgtttcc gcaacgaata tgaagccttt aatggcgaat ttttcattga agactgccgt   1380

aaagatgaaa tccgcctgac ctggaaaaaa gacgataaac gctgtagcct gtttattgat   1440

ctgaaaacct acaaaacgac gattgattac attaacgaaa acggtgaaga agtgaaatac   1500

ctggtttaa                                                           1509


<210>  4
<211>  502
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  4

Met Gly Gly Ser His His His His His His Gly Met Ala Ser Met Ala 
1               5                   10                  15      


Leu Lys Asn Lys Val Gln Leu Ile Thr Tyr Pro Asp Ser Leu Gly Gly 
            20                  25                  30          


Asn Leu Lys Thr Leu Asn Asp Val Leu Glu Lys Tyr Phe Ser Asp Val 
        35                  40                  45              


Phe Gly Gly Val His Ile Leu Pro Pro Phe Pro Ser Ser Gly Asp Arg 
    50                  55                  60                  


Gly Phe Ala Pro Ile Thr Tyr Ser Glu Ile Glu Pro Lys Phe Gly Thr 
65                  70                  75                  80  


Trp Tyr Asp Ile Lys Lys Met Ala Glu Asn Phe Asp Ile Leu Leu Asp 
                85                  90                  95      


Leu Met Val Asn His Val Ser Arg Arg Ser Ile Tyr Phe Gln Asp Phe 
            100                 105                 110         


Leu Lys Lys Gly Arg Lys Ser Glu Tyr Ala Asp Met Phe Ile Thr Leu 
        115                 120                 125             


Asp Lys Leu Trp Lys Asp Gly Lys Pro Val Lys Gly Asp Ile Glu Lys 
    130                 135                 140                 


Met Phe Leu Arg Arg Thr Leu Pro Tyr Ser Thr Phe Lys Ile Glu Glu 
145                 150                 155                 160 


Thr Gly Glu Glu Glu Lys Val Trp Thr Thr Phe Gly Lys Thr Asp Pro 
                165                 170                 175     


Ser Glu Gln Ile Asp Leu Asp Val Asn Ser His Leu Val Arg Glu Phe 
            180                 185                 190         


Leu Leu Glu Val Phe Lys Thr Phe Ser Asn Phe Gly Val Lys Ile Val 
        195                 200                 205             


Arg Leu Asp Ala Val Gly Tyr Val Ile Lys Lys Ile Gly Thr Ser Cys 
    210                 215                 220                 


Phe Phe Val Glu Pro Glu Ile Tyr Glu Phe Leu Asp Trp Ala Lys Gly 
225                 230                 235                 240 


Gln Ala Ala Ser Tyr Gly Ile Glu Leu Leu Leu Glu Val His Ser Gln 
                245                 250                 255     


Phe Glu Val Gln Tyr Lys Leu Ala Glu Arg Gly Phe Leu Ile Tyr Asp 
            260                 265                 270         


Phe Ile Leu Pro Phe Thr Val Leu Tyr Thr Leu Ile Asn Lys Ser Asn 
        275                 280                 285             


Glu Met Leu Tyr His Tyr Leu Lys Asn Arg Pro Ile Asn Gln Phe Thr 
    290                 295                 300                 


Met Leu Asp Cys His Asp Gly Ile Pro Val Lys Pro Asp Leu Asp Gly 
305                 310                 315                 320 


Leu Ile Asp Thr Lys Lys Ala Lys Glu Val Val Asp Ile Cys Val Gln 
                325                 330                 335     


Arg Gly Ala Asn Leu Ser Leu Ile Tyr Gly Asp Lys Tyr Lys Ser Glu 
            340                 345                 350         


Asp Gly Phe Asp Val His Gln Ile Asn Cys Thr Tyr Tyr Ser Ala Leu 
        355                 360                 365             


Asn Cys Asp Asp Asp Ala Tyr Leu Ala Ala Arg Ala Ile Gln Phe Phe 
    370                 375                 380                 


Thr Pro Gly Ile Pro Gln Val Tyr Tyr Val Gly Leu Leu Ala Gly Val 
385                 390                 395                 400 


Asn Asp Phe Glu Ala Val Lys Lys Thr Lys Glu Gly Arg Glu Ile Asn 
                405                 410                 415     


Arg His Asn Tyr Gly Leu Lys Glu Ile Glu Glu Ser Val Gln Lys Asn 
            420                 425                 430         


Val Val Gln Arg Leu Leu Lys Leu Ile Arg Phe Arg Asn Glu Tyr Glu 
        435                 440                 445             


Ala Phe Asn Gly Glu Phe Phe Ile Glu Asp Cys Arg Lys Asp Glu Ile 
    450                 455                 460                 


Arg Leu Thr Trp Lys Lys Asp Asp Lys Arg Cys Ser Leu Phe Ile Asp 
465                 470                 475                 480 


Leu Lys Thr Tyr Lys Thr Thr Ile Asp Tyr Ile Asn Glu Asn Gly Glu 
                485                 490                 495     


Glu Val Lys Tyr Leu Val 
            500         


<210>  5
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Chemically synthesized nucleic acid

<400>  5
gaattcggag gaaacaaaga tgggcggttc gcaccaccac caccaccacg gcatggctag     60

c                                                                     61


