                         LISTAGE DES SEQUENCES

<110>  Commissariat  l'nergie atomique et aux nergies 
       alternatives (CEA)
 
<120>  PROCEDE DE PREPARATION DE PROTEINE C1Q RECOMBINANTE

<130>  F263-529

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  223
<212>  PRT
<213>  Homo sapiens

<400>  1

Glu Asp Leu Cys Arg Ala Pro Asp Gly Lys Lys Gly Glu Ala Gly Arg 
1               5                   10                  15      


Pro Gly Arg Arg Gly Arg Pro Gly Leu Lys Gly Glu Gln Gly Glu Pro 
            20                  25                  30          


Gly Ala Pro Gly Ile Arg Thr Gly Ile Gln Gly Leu Lys Gly Asp Gln 
        35                  40                  45              


Gly Glu Pro Gly Pro Ser Gly Asn Pro Gly Lys Val Gly Tyr Pro Gly 
    50                  55                  60                  


Pro Ser Gly Pro Leu Gly Ala Arg Gly Ile Pro Gly Ile Lys Gly Thr 
65                  70                  75                  80  


Lys Gly Ser Pro Gly Asn Ile Lys Asp Gln Pro Arg Pro Ala Phe Ser 
                85                  90                  95      


Ala Ile Arg Arg Asn Pro Pro Met Gly Gly Asn Val Val Ile Phe Asp 
            100                 105                 110         


Thr Val Ile Thr Asn Gln Glu Glu Pro Tyr Gln Asn His Ser Gly Arg 
        115                 120                 125             


Phe Val Cys Thr Val Pro Gly Tyr Tyr Tyr Phe Thr Phe Gln Val Leu 
    130                 135                 140                 


Ser Gln Trp Glu Ile Cys Leu Ser Ile Val Ser Ser Ser Arg Gly Gln 
145                 150                 155                 160 


Val Arg Arg Ser Leu Gly Phe Cys Asp Thr Thr Asn Lys Gly Leu Phe 
                165                 170                 175     


Gln Val Val Ser Gly Gly Met Val Leu Gln Leu Gln Gln Gly Asp Gln 
            180                 185                 190         


Val Trp Val Glu Lys Asp Pro Lys Lys Gly His Ile Tyr Gln Gly Ser 
        195                 200                 205             


Glu Ala Asp Ser Val Phe Ser Gly Phe Leu Ile Phe Pro Ser Ala 
    210                 215                 220             


<210>  2
<211>  245
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Glu Gly Pro Arg Gly Trp Leu Val Leu Cys Val Leu Ala Ile Ser 
1               5                   10                  15      


Leu Ala Ser Met Val Thr Glu Asp Leu Cys Arg Ala Pro Asp Gly Lys 
            20                  25                  30          


Lys Gly Glu Ala Gly Arg Pro Gly Arg Arg Gly Arg Pro Gly Leu Lys 
        35                  40                  45              


Gly Glu Gln Gly Glu Pro Gly Ala Pro Gly Ile Arg Thr Gly Ile Gln 
    50                  55                  60                  


Gly Leu Lys Gly Asp Gln Gly Glu Pro Gly Pro Ser Gly Asn Pro Gly 
65                  70                  75                  80  


Lys Val Gly Tyr Pro Gly Pro Ser Gly Pro Leu Gly Ala Arg Gly Ile 
                85                  90                  95      


Pro Gly Ile Lys Gly Thr Lys Gly Ser Pro Gly Asn Ile Lys Asp Gln 
            100                 105                 110         


Pro Arg Pro Ala Phe Ser Ala Ile Arg Arg Asn Pro Pro Met Gly Gly 
        115                 120                 125             


Asn Val Val Ile Phe Asp Thr Val Ile Thr Asn Gln Glu Glu Pro Tyr 
    130                 135                 140                 


Gln Asn His Ser Gly Arg Phe Val Cys Thr Val Pro Gly Tyr Tyr Tyr 
145                 150                 155                 160 


Phe Thr Phe Gln Val Leu Ser Gln Trp Glu Ile Cys Leu Ser Ile Val 
                165                 170                 175     


Ser Ser Ser Arg Gly Gln Val Arg Arg Ser Leu Gly Phe Cys Asp Thr 
            180                 185                 190         


Thr Asn Lys Gly Leu Phe Gln Val Val Ser Gly Gly Met Val Leu Gln 
        195                 200                 205             


Leu Gln Gln Gly Asp Gln Val Trp Val Glu Lys Asp Pro Lys Lys Gly 
    210                 215                 220                 


His Ile Tyr Gln Gly Ser Glu Ala Asp Ser Val Phe Ser Gly Phe Leu 
225                 230                 235                 240 


Ile Phe Pro Ser Ala 
                245 


<210>  3
<211>  228
<212>  PRT
<213>  Homo sapiens

<400>  3

Gln Ala Gln Leu Ser Cys Thr Gly Pro Pro Ala Ile Pro Gly Ile Pro 
1               5                   10                  15      


Gly Ile Pro Gly Thr Pro Gly Pro Asp Gly Gln Pro Gly Thr Pro Gly 
            20                  25                  30          


Ile Lys Gly Glu Lys Gly Leu Pro Gly Leu Ala Gly Asp His Gly Glu 
        35                  40                  45              


Phe Gly Glu Lys Gly Asp Pro Gly Ile Pro Gly Asn Pro Gly Lys Val 
    50                  55                  60                  


Gly Pro Lys Gly Pro Met Gly Pro Lys Gly Gly Pro Gly Ala Pro Gly 
65                  70                  75                  80  


Ala Pro Gly Pro Lys Gly Glu Ser Gly Asp Tyr Lys Ala Thr Gln Lys 
                85                  90                  95      


Ile Ala Phe Ser Ala Thr Arg Thr Ile Asn Val Pro Leu Arg Arg Asp 
            100                 105                 110         


Gln Thr Ile Arg Phe Asp His Val Ile Thr Asn Met Asn Asn Asn Tyr 
        115                 120                 125             


Glu Pro Arg Ser Gly Lys Phe Thr Cys Lys Val Pro Gly Leu Tyr Tyr 
    130                 135                 140                 


Phe Thr Tyr His Ala Ser Ser Arg Gly Asn Leu Cys Val Asn Leu Met 
145                 150                 155                 160 


Arg Gly Arg Glu Arg Ala Gln Lys Val Val Thr Phe Cys Asp Tyr Ala 
                165                 170                 175     


Tyr Asn Thr Phe Gln Val Thr Thr Gly Gly Met Val Leu Lys Leu Glu 
            180                 185                 190         


Gln Gly Glu Asn Val Phe Leu Gln Ala Thr Asp Lys Asn Ser Leu Leu 
        195                 200                 205             


Gly Met Glu Gly Ala Asn Ser Ile Phe Ser Gly Phe Leu Leu Phe Pro 
    210                 215                 220                 


Asp Met Glu Ala 
225             


<210>  4
<211>  251
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Lys Ile Pro Trp Gly Ser Ile Pro Val Leu Met Leu Leu Leu Leu 
1               5                   10                  15      


Leu Gly Leu Ile Asp Ile Ser Gln Ala Gln Leu Ser Cys Thr Gly Pro 
            20                  25                  30          


Pro Ala Ile Pro Gly Ile Pro Gly Ile Pro Gly Thr Pro Gly Pro Asp 
        35                  40                  45              


Gly Gln Pro Gly Thr Pro Gly Ile Lys Gly Glu Lys Gly Leu Pro Gly 
    50                  55                  60                  


Leu Ala Gly Asp His Gly Glu Phe Gly Glu Lys Gly Asp Pro Gly Ile 
65                  70                  75                  80  


Pro Gly Asn Pro Gly Lys Val Gly Pro Lys Gly Pro Met Gly Pro Lys 
                85                  90                  95      


Gly Gly Pro Gly Ala Pro Gly Ala Pro Gly Pro Lys Gly Glu Ser Gly 
            100                 105                 110         


Asp Tyr Lys Ala Thr Gln Lys Ile Ala Phe Ser Ala Thr Arg Thr Ile 
        115                 120                 125             


Asn Val Pro Leu Arg Arg Asp Gln Thr Ile Arg Phe Asp His Val Ile 
    130                 135                 140                 


Thr Asn Met Asn Asn Asn Tyr Glu Pro Arg Ser Gly Lys Phe Thr Cys 
145                 150                 155                 160 


Lys Val Pro Gly Leu Tyr Tyr Phe Thr Tyr His Ala Ser Ser Arg Gly 
                165                 170                 175     


Asn Leu Cys Val Asn Leu Met Arg Gly Arg Glu Arg Ala Gln Lys Val 
            180                 185                 190         


Val Thr Phe Cys Asp Tyr Ala Tyr Asn Thr Phe Gln Val Thr Thr Gly 
        195                 200                 205             


Gly Met Val Leu Lys Leu Glu Gln Gly Glu Asn Val Phe Leu Gln Ala 
    210                 215                 220                 


Thr Asp Lys Asn Ser Leu Leu Gly Met Glu Gly Ala Asn Ser Ile Phe 
225                 230                 235                 240 


Ser Gly Phe Leu Leu Phe Pro Asp Met Glu Ala 
                245                 250     


<210>  5
<211>  217
<212>  PRT
<213>  Homo sapiens

<400>  5

Asn Thr Gly Cys Tyr Gly Ile Pro Gly Met Pro Gly Leu Pro Gly Ala 
1               5                   10                  15      


Pro Gly Lys Asp Gly Tyr Asp Gly Leu Pro Gly Pro Lys Gly Glu Pro 
            20                  25                  30          


Gly Ile Pro Ala Ile Pro Gly Ile Arg Gly Pro Lys Gly Gln Lys Gly 
        35                  40                  45              


Glu Pro Gly Leu Pro Gly His Pro Gly Lys Asn Gly Pro Met Gly Pro 
    50                  55                  60                  


Pro Gly Met Pro Gly Val Pro Gly Pro Met Gly Ile Pro Gly Glu Pro 
65                  70                  75                  80  


Gly Glu Glu Gly Arg Tyr Lys Gln Lys Phe Gln Ser Val Phe Thr Val 
                85                  90                  95      


Thr Arg Gln Thr His Gln Pro Pro Ala Pro Asn Ser Leu Ile Arg Phe 
            100                 105                 110         


Asn Ala Val Leu Thr Asn Pro Gln Gly Asp Tyr Asp Thr Ser Thr Gly 
        115                 120                 125             


Lys Phe Thr Cys Lys Val Pro Gly Leu Tyr Tyr Phe Val Tyr His Ala 
    130                 135                 140                 


Ser His Thr Ala Asn Leu Cys Val Leu Leu Tyr Arg Ser Gly Val Lys 
145                 150                 155                 160 


Val Val Thr Phe Cys Gly His Thr Ser Lys Thr Asn Gln Val Asn Ser 
                165                 170                 175     


Gly Gly Val Leu Leu Arg Leu Gln Val Gly Glu Glu Val Trp Leu Ala 
            180                 185                 190         


Val Asn Asp Tyr Tyr Asp Met Val Gly Ile Gln Gly Ser Asp Ser Val 
        195                 200                 205             


Phe Ser Gly Phe Leu Leu Phe Pro Asp 
    210                 215         


<210>  6
<211>  245
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Asp Val Gly Pro Ser Ser Leu Pro His Leu Gly Leu Lys Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Ala Leu Arg Gly Gln Ala Asn Thr Gly Cys 
            20                  25                  30          


Tyr Gly Ile Pro Gly Met Pro Gly Leu Pro Gly Ala Pro Gly Lys Asp 
        35                  40                  45              


Gly Tyr Asp Gly Leu Pro Gly Pro Lys Gly Glu Pro Gly Ile Pro Ala 
    50                  55                  60                  


Ile Pro Gly Ile Arg Gly Pro Lys Gly Gln Lys Gly Glu Pro Gly Leu 
65                  70                  75                  80  


Pro Gly His Pro Gly Lys Asn Gly Pro Met Gly Pro Pro Gly Met Pro 
                85                  90                  95      


Gly Val Pro Gly Pro Met Gly Ile Pro Gly Glu Pro Gly Glu Glu Gly 
            100                 105                 110         


Arg Tyr Lys Gln Lys Phe Gln Ser Val Phe Thr Val Thr Arg Gln Thr 
        115                 120                 125             


His Gln Pro Pro Ala Pro Asn Ser Leu Ile Arg Phe Asn Ala Val Leu 
    130                 135                 140                 


Thr Asn Pro Gln Gly Asp Tyr Asp Thr Ser Thr Gly Lys Phe Thr Cys 
145                 150                 155                 160 


Lys Val Pro Gly Leu Tyr Tyr Phe Val Tyr His Ala Ser His Thr Ala 
                165                 170                 175     


Asn Leu Cys Val Leu Leu Tyr Arg Ser Gly Val Lys Val Val Thr Phe 
            180                 185                 190         


Cys Gly His Thr Ser Lys Thr Asn Gln Val Asn Ser Gly Gly Val Leu 
        195                 200                 205             


Leu Arg Leu Gln Val Gly Glu Glu Val Trp Leu Ala Val Asn Asp Tyr 
    210                 215                 220                 


Tyr Asp Met Val Gly Ile Gln Gly Ser Asp Ser Val Phe Ser Gly Phe 
225                 230                 235                 240 


Leu Leu Phe Pro Asp 
                245 


<210>  7
<211>  253
<212>  PRT
<213>  Squence artificielle

<220>
<223>  Sous-unit C1qC+squence additionnelle d'acides amins

<400>  7

Met Asp Val Gly Pro Ser Ser Leu Pro His Leu Gly Leu Lys Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Ala Leu Arg Gly Gln Ala Asn Thr Gly Cys 
            20                  25                  30          


Tyr Gly Ile Pro Gly Met Pro Gly Leu Pro Gly Ala Pro Gly Lys Asp 
        35                  40                  45              


Gly Tyr Asp Gly Leu Pro Gly Pro Lys Gly Glu Pro Gly Ile Pro Ala 
    50                  55                  60                  


Ile Pro Gly Ile Arg Gly Pro Lys Gly Gln Lys Gly Glu Pro Gly Leu 
65                  70                  75                  80  


Pro Gly His Pro Gly Lys Asn Gly Pro Met Gly Pro Pro Gly Met Pro 
                85                  90                  95      


Gly Val Pro Gly Pro Met Gly Ile Pro Gly Glu Pro Gly Glu Glu Gly 
            100                 105                 110         


Arg Tyr Lys Gln Lys Phe Gln Ser Val Phe Thr Val Thr Arg Gln Thr 
        115                 120                 125             


His Gln Pro Pro Ala Pro Asn Ser Leu Ile Arg Phe Asn Ala Val Leu 
    130                 135                 140                 


Thr Asn Pro Gln Gly Asp Tyr Asp Thr Ser Thr Gly Lys Phe Thr Cys 
145                 150                 155                 160 


Lys Val Pro Gly Leu Tyr Tyr Phe Val Tyr His Ala Ser His Thr Ala 
                165                 170                 175     


Asn Leu Cys Val Leu Leu Tyr Arg Ser Gly Val Lys Val Val Thr Phe 
            180                 185                 190         


Cys Gly His Thr Ser Lys Thr Asn Gln Val Asn Ser Gly Gly Val Leu 
        195                 200                 205             


Leu Arg Leu Gln Val Gly Glu Glu Val Trp Leu Ala Val Asn Asp Tyr 
    210                 215                 220                 


Tyr Asp Met Val Gly Ile Gln Gly Ser Asp Ser Val Phe Ser Gly Phe 
225                 230                 235                 240 


Leu Leu Phe Pro Asp Asp Tyr Lys Asp Asp Asp Asp Lys 
                245                 250             


<210>  8
<211>  8
<212>  PRT
<213>  Squence artificielle

<220>
<223>  Squence additionnelle d'acides amins

<400>  8

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  9
<211>  24
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qA

<400>  9
ctagctagca tggagggtcc ccgg                                              24


<210>  10
<211>  22
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qA

<400>  10
ccggaattct caggcagatg gg                                                22


<210>  11
<211>  24
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qB

<400>  11
ctagctagca tgaagatccc atgg                                              24


<210>  12
<211>  21
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qB

<400>  12
cgcggatcct caggcctcca t                                                 21


<210>  13
<211>  24
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qC

<400>  13
ctagctagca tggacgtggg gccc                                              24


<210>  14
<211>  25
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qC

<400>  14
ccggaattcc tagtcgggga agagc                                             25


<210>  15
<211>  65
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR pour mutagnse dirige de C1qC

<400>  15
ggcttcctgc tcttccccga cgattacaag gatgacgacg ataagtagga gttctgcaga       60

tatcc                                                                   65


<210>  16
<211>  65
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR pour mutagnse dirige de C1qC

<400>  16
ggatatctgc agaactccta cttatcgtcg tcatccttgt aatcgtcggg gaagagcagg       60

aagcc                                                                   65


<210>  17
<211>  10
<212>  PRT
<213>  Squence artificielle

<220>
<223>  Squence additionnelle d'acides amins

<400>  17

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  18
<211>  60
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qC

<400>  18
cccctcaggg gccaagccga ttacaaggat gacgacgata agaacacagg ctgctacggg       60


<210>  19
<211>  60
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qC

<400>  19
cccgtagcag cctgtgttct tatcgtcgtc atccttgtaa tcggcttggc ccctgagggg       60


<210>  20
<211>  6
<212>  PRT
<213>  Squence artificielle

<220>
<223>  Etiquette 6 histidines

<400>  20

His His His His His His 
1               5       


<210>  21
<211>  51
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qC

<400>  21
gctcttcccc gaccatcacc atcaccatca ctaggagttc tgcagatatc c                51


<210>  22
<211>  51
<212>  DNA
<213>  Squence artificielle

<220>
<223>  Amorce PCR d'amplification de C1qC

<400>  22
ggatatctgc agaactccta gtgatggtga tggtgatggt cggggaagag c                51


<210>  23
<211>  253
<212>  PRT
<213>  Squence artificielle

<220>
<223>  Sous-unit C1qC+squence additionnelle d'acides amins en 
       N-terminal

<400>  23

Met Asp Val Gly Pro Ser Ser Leu Pro His Leu Gly Leu Lys Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Ala Leu Arg Gly Gln Ala Asp Tyr Lys Asp 
            20                  25                  30          


Asp Asp Asp Lys Asn Thr Gly Cys Tyr Gly Ile Pro Gly Met Pro Gly 
        35                  40                  45              


Leu Pro Gly Ala Pro Gly Lys Asp Gly Tyr Asp Gly Leu Pro Gly Pro 
    50                  55                  60                  


Lys Gly Glu Pro Gly Ile Pro Ala Ile Pro Gly Ile Arg Gly Pro Lys 
65                  70                  75                  80  


Gly Gln Lys Gly Glu Pro Gly Leu Pro Gly His Pro Gly Lys Asn Gly 
                85                  90                  95      


Pro Met Gly Pro Pro Gly Met Pro Gly Val Pro Gly Pro Met Gly Ile 
            100                 105                 110         


Pro Gly Glu Pro Gly Glu Glu Gly Arg Tyr Lys Gln Lys Phe Gln Ser 
        115                 120                 125             


Val Phe Thr Val Thr Arg Gln Thr His Gln Pro Pro Ala Pro Asn Ser 
    130                 135                 140                 


Leu Ile Arg Phe Asn Ala Val Leu Thr Asn Pro Gln Gly Asp Tyr Asp 
145                 150                 155                 160 


Thr Ser Thr Gly Lys Phe Thr Cys Lys Val Pro Gly Leu Tyr Tyr Phe 
                165                 170                 175     


Val Tyr His Ala Ser His Thr Ala Asn Leu Cys Val Leu Leu Tyr Arg 
            180                 185                 190         


Ser Gly Val Lys Val Val Thr Phe Cys Gly His Thr Ser Lys Thr Asn 
        195                 200                 205             


Gln Val Asn Ser Gly Gly Val Leu Leu Arg Leu Gln Val Gly Glu Glu 
    210                 215                 220                 


Val Trp Leu Ala Val Asn Asp Tyr Tyr Asp Met Val Gly Ile Gln Gly 
225                 230                 235                 240 


Ser Asp Ser Val Phe Ser Gly Phe Leu Leu Phe Pro Asp 
                245                 250             


<210>  24
<211>  251
<212>  PRT
<213>  Squence artificielle

<220>
<223>  Sous-unit C1qC+6His

<400>  24

Met Asp Val Gly Pro Ser Ser Leu Pro His Leu Gly Leu Lys Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Ala Leu Arg Gly Gln Ala Asn Thr Gly Cys 
            20                  25                  30          


Tyr Gly Ile Pro Gly Met Pro Gly Leu Pro Gly Ala Pro Gly Lys Asp 
        35                  40                  45              


Gly Tyr Asp Gly Leu Pro Gly Pro Lys Gly Glu Pro Gly Ile Pro Ala 
    50                  55                  60                  


Ile Pro Gly Ile Arg Gly Pro Lys Gly Gln Lys Gly Glu Pro Gly Leu 
65                  70                  75                  80  


Pro Gly His Pro Gly Lys Asn Gly Pro Met Gly Pro Pro Gly Met Pro 
                85                  90                  95      


Gly Val Pro Gly Pro Met Gly Ile Pro Gly Glu Pro Gly Glu Glu Gly 
            100                 105                 110         


Arg Tyr Lys Gln Lys Phe Gln Ser Val Phe Thr Val Thr Arg Gln Thr 
        115                 120                 125             


His Gln Pro Pro Ala Pro Asn Ser Leu Ile Arg Phe Asn Ala Val Leu 
    130                 135                 140                 


Thr Asn Pro Gln Gly Asp Tyr Asp Thr Ser Thr Gly Lys Phe Thr Cys 
145                 150                 155                 160 


Lys Val Pro Gly Leu Tyr Tyr Phe Val Tyr His Ala Ser His Thr Ala 
                165                 170                 175     


Asn Leu Cys Val Leu Leu Tyr Arg Ser Gly Val Lys Val Val Thr Phe 
            180                 185                 190         


Cys Gly His Thr Ser Lys Thr Asn Gln Val Asn Ser Gly Gly Val Leu 
        195                 200                 205             


Leu Arg Leu Gln Val Gly Glu Glu Val Trp Leu Ala Val Asn Asp Tyr 
    210                 215                 220                 


Tyr Asp Met Val Gly Ile Gln Gly Ser Asp Ser Val Phe Ser Gly Phe 
225                 230                 235                 240 


Leu Leu Phe Pro Asp His His His His His His 
                245                 250     


