                         SEQUENCE LISTING

<110>  Evocatal GmbH
 
<120>  Variants of the Parvibaculum lavamentivorans short-chain alcohol 
       dehydrogenase

<130>  ED 40325

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  807
<212>  DNA
<213>  Parvibaculum lavamentivorans

<400>  1
atggctcgta tgcagggcaa ggtagcgatc gtcacgggcg cggcaaaggg gatcggggcg       60

gcgaccgcca aggcgctggc acgcgagggg gcgaaggtcg tgtgcagcga tttcgacgcg      120

gagaacggcc aggttgtcgc cgacgaaatc gcgggcgagg gtgggacggc aaccttcttc      180

cggcatgacg tgaccgacga ggcgcaatgg gaagccgtgg tgaagctggc ggaggagaaa      240

ttcggcgggc ttcatgtggt tgtgaacaat gcaggcatcg cgccggaggc cggccccatc      300

gaggagaaga cgctggccga ctggcggcac acgatcgagg tcgatctcga cagcgtgttt      360

cttggctgca aacacggcat ccgcacgatc aagaaatata cgtcgaaggg cggcgcgggc      420

ggtgcaatcg tcaacatttc ctcgatcctc ggacttgtcg ggcagtccgg cgccagcgac      480

tacaatgcgg cgaagggggg cgtcaggctt cttaccaaat cggctgcgct ggaatgcgcc      540

gatgcgggct acaatattcg cgtgaactcc gtgcatcccg gctacataga tacgccgatg      600

gtgaagaacg tcatcaatcg cggcgtgatg ggcggtgcga ccgtaggcgc gaacgagatg      660

cgcgaaatgc tgatcatgca gcatccgatc ggaaggctcg gtattgcgga ggaaattgca      720

aacgcgatcc tgttcctgtc gagcgacgaa tccaccttca tgaccggatc ggaagtcgtg      780

gtggatgggg gatatacgac ccgctag                                          807


<210>  2
<211>  268
<212>  PRT
<213>  Parvibaculum lavamentivorans

<400>  2

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Asn Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  3
<211>  810
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence based on coding sequence of the wildtype gene
       for short-chain alcohol dehydrogenase from P. lavamentivorans, 
       but codon usage optimized for expression in E. coli.

<400>  3
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tataatgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaaaaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgc tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  4
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide encoded by geneart master DNA; identical amino acid 
       sequence with the P. lavamentivorans wildtype short-chain alcohol
       dehydrogenase .

<400>  4

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Asn Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  5
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein A at position 158 is 
       replaced with K, and N at position 162 is replaced with M.

<400>  5
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg taagagcgat      480

tatatggctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaaaaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgc tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  6
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein A at 
       position 158 is replaced with K, and N at position 162 is 
       replaced with M.

<400>  6

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Lys Ser Asp 
145                 150                 155                 160 


Tyr Met Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  7
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein N at position 162 is 
       replaced with P.

<400>  7
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tatcccgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaaaaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgc tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  8
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein N at 
       position 162 is replaced with P.

<400>  8

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Pro Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  9
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein A at position 158 is 
       replaced with H, and N at position 162 is replaced with P.

<400>  9
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tcacagcgat      480

tatcccgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaaaaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgc tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  10
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein A at 
       position 158 is replaced with H, and N at position 162 is 
       replaced with P.

<400>  10

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly His Ser Asp 
145                 150                 155                 160 


Tyr Pro Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  11
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein N at 
       position 162 is replaced with A.

<400>  11
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tatgcggctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaaaaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgc tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  12
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein N at 
       position 162 is replaced with A.

<400>  12

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Ala Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  13
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein A at position 158 is 
       replaced with D, and N at position 162 is replaced with Q.

<400>  13
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgacagcgat      480

tatcargctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaaaaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgc tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  14
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein A at 
       position 158 is replaced with D, and N at position 162 is 
       replaced with Q.

<400>  14

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Asp Ser Asp 
145                 150                 155                 160 


Tyr Gln Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  15
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein A at position 158 is 
       replaced with D, and Nat position 162 is replaced with G.


<220>
<221>  misc_feature
<222>  (486)..(486)
<223>  n is a, c, g, or t

<400>  15
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgacagcgat      480

tatggngctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaaaaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgc tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  16
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein A at 
       position 158 is replaced with D, and N at position 162 is 
       replaced with G.

<400>  16

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Asp Ser Asp 
145                 150                 155                 160 


Tyr Gly Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Lys Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Leu 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  17
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein K at position 202 is 
       replaced with R, and L at position 224 is replaced with W.


<220>
<221>  misc_feature
<222>  (606)..(606)
<223>  n is a, c, g, or t

<400>  17
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tataatgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcgnaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  18
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein K at 
       position 202 is replaced with R, and L at position 224 is 
       replaced with W.

<400>  18

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Asn Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Arg Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  19
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein K at position 202 is 
       replaced with Q, and L at position 224 is replaced with W.

<400>  19
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tataatgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcaraacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  20
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein K at 
       position 202 is replaced with Q, and L at position 224 is 
       replaced with W.

<400>  20

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Asn Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Gln Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  21
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein K at position 202 is 
       replaced with S, and L at position 224 is replaced with Y.


<220>
<221>  misc_feature
<222>  (606)..(606)
<223>  n is a, c, g, or t

<400>  21
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tataatgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgtcnaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ayattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  22
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein K at 
       position 202 is replaced with S, and L at position 224 is 
       replaced with Y.

<400>  22

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Asn Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Ser Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Tyr 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  23
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein K at position 202 is 
       replaced with N, and L at position 224 is replaced with M.

<400>  23
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tataatgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgaayaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatga tgattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  24
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein Kat 
       position 202 is replaced with N, and L at position 224 is 
       replaced with M.

<400>  24

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Asn Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Asn Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Met 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  25
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain ADH, wherein A at position 158 is replaced with K, N 
       at position 162 is replaced with M, K at position 202 is replaced
       with R, and L at position 224 is replaced with W.


<220>
<221>  misc_feature
<222>  (606)..(606)
<223>  n is a, c, g, or t

<400>  25
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg taagagcgat      480

tatatggctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcgnaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  26
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain ADH, wherein A at position 158 is 
       replaced with K, N at position 162 is replaced with M, K at 
       position 202 is replaced with R, and L at position 224 is 
       

<400>  26

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Lys Ser Asp 
145                 150                 155                 160 


Tyr Met Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Arg Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  27
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain alcohol dehydrogenase, wherein N at position 162 is 
       replaced with A, K at position 202 is replaced with R, and L at 
       position 224 is replaced with W.


<220>
<221>  misc_feature
<222>  (606)..(606)
<223>  n is a, c, g, or t

<400>  27
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tatgcggctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcgnaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  28
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain alcohol dehydrogenase, wherein N at 
       position 162 is replaced with A, K at position 202 is replaced 
       with R, and Lat position 224 is replaced with W.

<400>  28

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Ala Ser Asp 
145                 150                 155                 160 


Tyr Ala Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Arg Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  29
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain ADH, wherein A at position 158 is replaced with D, N 
       at position 162 is replaced with S, K at position 202 is replaced
       with R, and L at position 224 is replaced with W.


<220>
<221>  misc_feature
<222>  (606)..(606)
<223>  n is a, c, g, or t

<400>  29
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgayggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgacagcgat      480

tattccgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcgnaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  30
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain ADH, wherein A at position 158 is 
       replaced with D, N at position 162 is replaced with S, K at 
       position 202 is replaced with R, and L at position 224 is 
       

<400>  30

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Asp Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Asp Ser Asp 
145                 150                 155                 160 


Tyr Ser Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Arg Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  31
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain ADH, wherein A at position 158 is replaced with D, N 
       at position 162 is replaced with G, K at position 202 is replaced
       with R, and L at position 224 is replaced with W.


<220>
<221>  misc_feature
<222>  (606)..(606)
<223>  n is a, c, g, or t

<400>  31
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtgccggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgacagcgat      480

tatggcgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcgnaacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  32
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain ADH, wherein A at position 158 is 
       replaced with D, N at position 162 is replaced with G, K at 
       position 202 is replaced with R, and L at position 224 is 
       

<400>  32

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly Ala Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Asp Ser Asp 
145                 150                 155                 160 


Tyr Gly Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Arg Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  33
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain ADH, wherein A at position 158 is replaced with H, N 
       at position 162 is replaced with G, K at position 202 is replaced
       with Q, and L at position 224 is replaced with W.


<220>
<221>  misc_feature
<222>  (486)..(486)
<223>  n is a, c, g, or t

<400>  33
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtcayggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgcaagcgat      480

tatggngctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcaraacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  34
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain ADH, wherein A at position 158 is 
       replaced with H, N at position 162 is replaced with G, K at 
       position 202 is replaced with Q, and L at position 224 is 
       

<400>  34

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly His Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly His Ser Asp 
145                 150                 155                 160 


Tyr Gly Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Gln Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  35
<211>  810
<212>  DNA
<213>  artificial

<220>
<223>  Nucleotide sequence encoding a variant of the P. lavamentivorans 
       short-chain ADH, wherein A at position 158 is replaced with D, N 
       at position 162 is replaced with C, K at position 202 is replaced
       with Q, and L at position 224 is replaced with W.

<400>  35
atggcacgta tgcagggtaa agttgcaatt gtgaccggtg cagcaaaagg tattggtgca       60

gcaaccgcaa aagcactggc acgtgaaggt gcaaaagttg tttgtagcga ttttgatgcc      120

gaaaatggcc aggttgttgc agatgaaatt gccggtgaag gtggcaccgc aacctttttt      180

cgccatgatg tgaccgatga agcacagtgg gaagcagttg tgaaactggc cgaagaaaaa      240

tttggtggtc tgcatgttgt ggtgaacaat gcaggtattg caccggaagc aggtccgatt      300

gaagaaaaaa ccctggcaga ttggcgtcat accattgaag ttgatctgga tagcgttttt      360

ctgggttgca aacatggtat tcgcaccatc aaaaaataca ccagcaaagg tggtcayggt      420

ggtgcaattg tgaatattag cagcattctg ggtctggttg gccagagcgg tgacagcgat      480

tattgcgctg ccaaaggtgg tgttcgtctg ctgaccaaaa gcgcagcact ggaatgtgca      540

gatgcaggtt ataacattcg tgtgaatagc gtgcatcctg gctatattga taccccgatg      600

gtgcaraacg tgatcaatcg tggtgtgatg ggtggtgcaa ccgttggtgc aaatgaaatg      660

cgtgaaatgt ggattatgca gcatccgatt ggtcgtctgg gtattgccga agaaattgca      720

aatgcaattc tgtttctgag cagtgatgaa agcaccttta tgaccggtag cgaagttgtt      780

gttgatggtg gttataccac ccgttaatga                                       810


<210>  36
<211>  268
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide having an amino acid sequence based on the P. 
       lavamentivorans short-chain ADH, wherein A at position 158 is 
       replaced with D, N at position 162 is replaced with C, K at 
       position 202 is replaced with Q, and L at position 224 is 
       

<400>  36

Met Ala Arg Met Gln Gly Lys Val Ala Ile Val Thr Gly Ala Ala Lys 
1               5                   10                  15      


Gly Ile Gly Ala Ala Thr Ala Lys Ala Leu Ala Arg Glu Gly Ala Lys 
            20                  25                  30          


Val Val Cys Ser Asp Phe Asp Ala Glu Asn Gly Gln Val Val Ala Asp 
        35                  40                  45              


Glu Ile Ala Gly Glu Gly Gly Thr Ala Thr Phe Phe Arg His Asp Val 
    50                  55                  60                  


Thr Asp Glu Ala Gln Trp Glu Ala Val Val Lys Leu Ala Glu Glu Lys 
65                  70                  75                  80  


Phe Gly Gly Leu His Val Val Val Asn Asn Ala Gly Ile Ala Pro Glu 
                85                  90                  95      


Ala Gly Pro Ile Glu Glu Lys Thr Leu Ala Asp Trp Arg His Thr Ile 
            100                 105                 110         


Glu Val Asp Leu Asp Ser Val Phe Leu Gly Cys Lys His Gly Ile Arg 
        115                 120                 125             


Thr Ile Lys Lys Tyr Thr Ser Lys Gly Gly His Gly Gly Ala Ile Val 
    130                 135                 140                 


Asn Ile Ser Ser Ile Leu Gly Leu Val Gly Gln Ser Gly Asp Ser Asp 
145                 150                 155                 160 


Tyr Cys Ala Ala Lys Gly Gly Val Arg Leu Leu Thr Lys Ser Ala Ala 
                165                 170                 175     


Leu Glu Cys Ala Asp Ala Gly Tyr Asn Ile Arg Val Asn Ser Val His 
            180                 185                 190         


Pro Gly Tyr Ile Asp Thr Pro Met Val Gln Asn Val Ile Asn Arg Gly 
        195                 200                 205             


Val Met Gly Gly Ala Thr Val Gly Ala Asn Glu Met Arg Glu Met Trp 
    210                 215                 220                 


Ile Met Gln His Pro Ile Gly Arg Leu Gly Ile Ala Glu Glu Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Phe Leu Ser Ser Asp Glu Ser Thr Phe Met Thr Gly 
                245                 250                 255     


Ser Glu Val Val Val Asp Gly Gly Tyr Thr Thr Arg 
            260                 265             


<210>  37
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplification of P. lavamentivorans A short-chain 
       alcohol dehydrogenase gene or gene fragment.

<400>  37
ttgtccagag ctcatatggc acgtatgcag gg                                     32


<210>  38
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplification of P. lavamentivorans short-chain 
       alcohol dehydrogenase gene or gene fragment.

<400>  38
atataagctt tcattaacgg gtggtataac c                                      31


<210>  39
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  39
gagcggtaag agcgattata tggctgccaa ag                                     32


<210>  40
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  40
ctttggcagc catataatcg ctcttaccgc tc                                     32


<210>  41
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  41
gagcggtcac agcgattatc ccgctgccaa ag                                     32


<210>  42
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  42
ctttggcagc gggataatcg ctgtgaccgc tc                                     32


<210>  43
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  43
gagcggtgcg agcgattatg ccgctgccaa ag                                     32


<210>  44
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  44
ctttggcagc ggcataatcg ctcgcaccgc tc                                     32


<210>  45
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  45
gagcggtgac agcgattatt ccgctgccaa ag                                     32


<210>  46
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  46
ctttggcagc ggaataatgc gtgtcaccgc tc                                     32


<210>  47
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  47
gagcggtgac agcgattatg gcgctgccaa ag                                     32


<210>  48
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  48
ctttggcagc gccataatcg ctgtcaccgc tc                                     32


<210>  49
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  49
gagcggtcac agcgattatg gcgctgccaa ag                                     32


<210>  50
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans ADH 200
       gene or gene fragment.

<400>  50
ctttggcagc gccataatcg ctgtgaccgc tc                                     32


<210>  51
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  51
gagcggtgac agcgattatt gcgctgccaa ag                                     32


<210>  52
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  Primer for amplifying and altering the P. lavamentivorans 
       short-chain alcohol dehydrogenase gene or gene fragment.

<400>  52
ctttggcagc gcaataatcg ctgtcaccgc tc                                     32


