                         SEQUENCE LISTING

<110>  BioAmber, Inc
       BioAmber, Inc
 
<120>  Pathways to adipate semialdehyde and other organic products

<130>  family 109 PCT

<140>  61/703,360
<141>  2012-09-20

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1185
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1
atgacccgtg aagtggttgt tgtgtctggc gttcgtaccg caatcggcac gtttggtggc       60

tccctgaaag atgtcgctcc ggctgaactg ggcgcgctgg ttgtgcgtga agcactggct      120

cgcgcgcagg tgtctggcga tgacgttggt catgtcgtgt ttggcaacgt gattcaaacc      180

gaaccgcgtg atatgtatct gggtcgcgtt gcggccgtca acggtggtgt gaccatcaat      240

gccccggcac tgacggttaa tcgtctgtgc ggcagcggtc tgcaagcgat tgtgagcgcg      300

gcgcaaacca tcctgctggg cgatacggac gttgcgattg gcggtggcgc agaaagcatg      360

tctcgcgccc cgtacctggc accggcggca cgttggggtg cacgcatggg cgatgctggt      420

ctggtggaca tgatgctggg cgcgctgcat gatccgtttc atcgtattca catgggtgtc      480

accgctgaaa acgtggcgaa agaatatgat attagccgtg cgcagcaaga cgaagcagct      540

ctggaatcac accgtcgcgc atcggcggcc attaaagcgg gctactttaa ggatcagatc      600

gtgccggttg tctcgaaagg ccgtaagggt gatgttacct tcgatacgga cgagcatgtg      660

cgtcacgacg cgaccattga tgacatgacg aaactgcgcc cggtcttcgt gaaggaaaat      720

ggcaccgtta cggctggcaa cgcgagtggt ctgaatgatg cagctgcggc cgtggttatg      780

atggaacgtg ctgaagcgga acgtcgcggt ctgaaaccgc tggcacgtct ggtgtcctat      840

ggccacgcag gtgttgaccc gaaagcaatg ggcattggtc cggtgccggc caccaagatc      900

gcactggaac gtgctggtct gcaagtttct gatctggacg tcattgaagc caacgaagca      960

tttgcagctc aggcctgcgc agtcacgaaa gcgctgggtc tggacccggc aaaggttaac     1020

ccgaatggca gtggtatttc cctgggtcat ccgatcggcg ctaccggtgc gctgatcacg     1080

gttaaagcac tgcacgaact gaatcgtgtc cagggtcgtt acgccctggt gaccatgtgt     1140

attggtggcg gtcaaggtat tgcggccatc ttcgaacgca tctaa                     1185


<210>  2
<211>  394
<212>  PRT
<213>  Cupriavidus necator

<400>  2

Met Thr Arg Glu Val Val Val Val Ser Gly Val Arg Thr Ala Ile Gly 
1               5                   10                  15      


Thr Phe Gly Gly Ser Leu Lys Asp Val Ala Pro Ala Glu Leu Gly Ala 
            20                  25                  30          


Leu Val Val Arg Glu Ala Leu Ala Arg Ala Gln Val Ser Gly Asp Asp 
        35                  40                  45              


Val Gly His Val Val Phe Gly Asn Val Ile Gln Thr Glu Pro Arg Asp 
    50                  55                  60                  


Met Tyr Leu Gly Arg Val Ala Ala Val Asn Gly Gly Val Thr Ile Asn 
65                  70                  75                  80  


Ala Pro Ala Leu Thr Val Asn Arg Leu Cys Gly Ser Gly Leu Gln Ala 
                85                  90                  95      


Ile Val Ser Ala Ala Gln Thr Ile Leu Leu Gly Asp Thr Asp Val Ala 
            100                 105                 110         


Ile Gly Gly Gly Ala Glu Ser Met Ser Arg Ala Pro Tyr Leu Ala Pro 
        115                 120                 125             


Ala Ala Arg Trp Gly Ala Arg Met Gly Asp Ala Gly Leu Val Asp Met 
    130                 135                 140                 


Met Leu Gly Ala Leu His Asp Pro Phe His Arg Ile His Met Gly Val 
145                 150                 155                 160 


Thr Ala Glu Asn Val Ala Lys Glu Tyr Asp Ile Ser Arg Ala Gln Gln 
                165                 170                 175     


Asp Glu Ala Ala Leu Glu Ser His Arg Arg Ala Ser Ala Ala Ile Lys 
            180                 185                 190         


Ala Gly Tyr Phe Lys Asp Gln Ile Val Pro Val Val Ser Lys Gly Arg 
        195                 200                 205             


Lys Gly Asp Val Thr Phe Asp Thr Asp Glu His Val Arg His Asp Ala 
    210                 215                 220                 


Thr Ile Asp Asp Met Thr Lys Leu Arg Pro Val Phe Val Lys Glu Asn 
225                 230                 235                 240 


Gly Thr Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Ala Ala Ala 
                245                 250                 255     


Ala Val Val Met Met Glu Arg Ala Glu Ala Glu Arg Arg Gly Leu Lys 
            260                 265                 270         


Pro Leu Ala Arg Leu Val Ser Tyr Gly His Ala Gly Val Asp Pro Lys 
        275                 280                 285             


Ala Met Gly Ile Gly Pro Val Pro Ala Thr Lys Ile Ala Leu Glu Arg 
    290                 295                 300                 


Ala Gly Leu Gln Val Ser Asp Leu Asp Val Ile Glu Ala Asn Glu Ala 
305                 310                 315                 320 


Phe Ala Ala Gln Ala Cys Ala Val Thr Lys Ala Leu Gly Leu Asp Pro 
                325                 330                 335     


Ala Lys Val Asn Pro Asn Gly Ser Gly Ile Ser Leu Gly His Pro Ile 
            340                 345                 350         


Gly Ala Thr Gly Ala Leu Ile Thr Val Lys Ala Leu His Glu Leu Asn 
        355                 360                 365             


Arg Val Gln Gly Arg Tyr Ala Leu Val Thr Met Cys Ile Gly Gly Gly 
    370                 375                 380                 


Gln Gly Ile Ala Ala Ile Phe Glu Arg Ile 
385                 390                 


<210>  3
<211>  1182
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3
atgacggacg ttgttattgt gtcggcggct cgcacggctg tcggcaaatt cggtggctct       60

ctggcaaaaa tcccggctcc ggaactgggt gcagtggtta ttaaagcagc actggaacgt      120

gcaggtgtga agccggaaca agtcagcgaa gtgatcatgg gtcaagttct gaccgcaggt      180

tctggtcaga acccggctcg tcaagcagct attaaagcag gcctgccggc tatggttccg      240

gcgatgacga tcaacaaagt ctgcggtagc ggtctgaagg cagtgatgct ggcagcaaat      300

gctattatgg cgggtgatgc cgaaatcgtc gtggccggcg gtcaggaaaa tatgtcagca      360

gctccgcatg ttctgccggg ttcgcgtgac ggctttcgca tgggtgatgc gaaactggtg      420

gataccatga ttgtggatgg cctgtgggac gtctataacc agtaccacat gggtattacc      480

gccgaaaatg ttgcaaaaga atatggcatc acccgtgaag cgcaggatga atttgccgtg      540

ggctctcaga acaaggcgga agcggcccaa aaagccggca agttcgacga agaaattgtt      600

ccggtcctga tcccgcagcg taaaggcgat ccggttgcct ttaagaccga cgaatttgtc      660

cgccaaggcg caacgctgga tagcatgtct ggtctgaaac cggcttttga caaggcaggc      720

accgtgacgg cagctaacgc gagtggcctg aatgatggtg cggccgcagt tgtcgtgatg      780

tccgctgcga aagcaaagga actgggtctg accccgctgg caacgattaa atcatacgct      840

aacgcgggtg ttgatccgaa ggtcatgggt atgggtccgg tgccggcaag taaacgtgca      900

ctgtcccgcg ctgaatggac cccgcaggat ctggacctga tggaaatcaa tgaagcgttc      960

gccgcacaag ccctggcagt tcatcagcaa atgggctggg atacctcaaa agtgaacgtt     1020

aatggcggtg ctattgcgat cggtcatccg attggtgcct cgggctgccg tatcctggtg     1080

acgctgctgc acgaaatgaa acgtcgcgat gcaaaaaagg gtctggcttc cctgtgtatt     1140

ggcggtggca tgggtgtggc actggcagtt gaacgcaaat aa                        1182


<210>  4
<211>  393
<212>  PRT
<213>  Ralstonia sp., strain EMBL EON20543.1

<400>  4

Met Thr Asp Val Val Ile Val Ser Ala Ala Arg Thr Ala Val Gly Lys 
1               5                   10                  15      


Phe Gly Gly Ser Leu Ala Lys Ile Pro Ala Pro Glu Leu Gly Ala Val 
            20                  25                  30          


Val Ile Lys Ala Ala Leu Glu Arg Ala Gly Val Lys Pro Glu Gln Val 
        35                  40                  45              


Ser Glu Val Ile Met Gly Gln Val Leu Thr Ala Gly Ser Gly Gln Asn 
    50                  55                  60                  


Pro Ala Arg Gln Ala Ala Ile Lys Ala Gly Leu Pro Ala Met Val Pro 
65                  70                  75                  80  


Ala Met Thr Ile Asn Lys Val Cys Gly Ser Gly Leu Lys Ala Val Met 
                85                  90                  95      


Leu Ala Ala Asn Ala Ile Met Ala Gly Asp Ala Glu Ile Val Val Ala 
            100                 105                 110         


Gly Gly Gln Glu Asn Met Ser Ala Ala Pro His Val Leu Pro Gly Ser 
        115                 120                 125             


Arg Asp Gly Phe Arg Met Gly Asp Ala Lys Leu Val Asp Thr Met Ile 
    130                 135                 140                 


Val Asp Gly Leu Trp Asp Val Tyr Asn Gln Tyr His Met Gly Ile Thr 
145                 150                 155                 160 


Ala Glu Asn Val Ala Lys Glu Tyr Gly Ile Thr Arg Glu Ala Gln Asp 
                165                 170                 175     


Glu Phe Ala Val Gly Ser Gln Asn Lys Ala Glu Ala Ala Gln Lys Ala 
            180                 185                 190         


Gly Lys Phe Asp Glu Glu Ile Val Pro Val Leu Ile Pro Gln Arg Lys 
        195                 200                 205             


Gly Asp Pro Val Ala Phe Lys Thr Asp Glu Phe Val Arg Gln Gly Ala 
    210                 215                 220                 


Thr Leu Asp Ser Met Ser Gly Leu Lys Pro Ala Phe Asp Lys Ala Gly 
225                 230                 235                 240 


Thr Val Thr Ala Ala Asn Ala Ser Gly Leu Asn Asp Gly Ala Ala Ala 
                245                 250                 255     


Val Val Val Met Ser Ala Ala Lys Ala Lys Glu Leu Gly Leu Thr Pro 
            260                 265                 270         


Leu Ala Thr Ile Lys Ser Tyr Ala Asn Ala Gly Val Asp Pro Lys Val 
        275                 280                 285             


Met Gly Met Gly Pro Val Pro Ala Ser Lys Arg Ala Leu Ser Arg Ala 
    290                 295                 300                 


Glu Trp Thr Pro Gln Asp Leu Asp Leu Met Glu Ile Asn Glu Ala Phe 
305                 310                 315                 320 


Ala Ala Gln Ala Leu Ala Val His Gln Gln Met Gly Trp Asp Thr Ser 
                325                 330                 335     


Lys Val Asn Val Asn Gly Gly Ala Ile Ala Ile Gly His Pro Ile Gly 
            340                 345                 350         


Ala Ser Gly Cys Arg Ile Leu Val Thr Leu Leu His Glu Met Lys Arg 
        355                 360                 365             


Arg Asp Ala Lys Lys Gly Leu Ala Ser Leu Cys Ile Gly Gly Gly Met 
    370                 375                 380                 


Gly Val Ala Leu Ala Val Glu Arg Lys 
385                 390             


<210>  5
<211>  1179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5
atgaaagaag tcgttatcgc tagtgctgtt cgcaccgcca ttggttccta cggcaaatcc       60

ctgaaagatg tcccggcagt tgacctgggt gcgaccgcca ttaaagaagc agtcaaaaag      120

gctggcatca aaccggaaga tgtcaacgaa gtgattctgg gtaacgtgct gcaagcaggt      180

ctgggtcaaa acccggcacg tcaggcctca tttaaagccg gcctgccggt ggaaattccg      240

gcaatgacca tcaataaagt gtgcggtagt ggtctgcgta cggtttccct ggcagcacaa      300

attatcaaag caggtgatgc tgacgttatt atcgcaggcg gtatggaaaa catgagccgt      360

gcgccgtatc tggcgaacaa tgcccgttgg ggttaccgca tgggcaacgc caaatttgtg      420

gatgaaatga ttaccgatgg tctgtgggac gcattcaatg attatcacat gggcattacg      480

gcggaaaaca tcgccgaacg ttggaatatc agccgcgaag aacaagacga atttgcactg      540

gctagccaga aaaaggcaga agaagctatt aaatctggcc agttcaagga tgaaattgtt      600

ccggtggtta tcaaaggtcg taagggcgaa accgtcgtgg atacggacga acacccgcgc      660

tttggttcta ccatcgaagg cctggcaaaa ctgaagccgg ctttcaaaaa ggacggcacc      720

gtgacggcgg gtaacgccag tggcctgaat gattgtgcag ctgttctggt cattatgtcc      780

gcagaaaaag ctaaggaact gggtgtgaaa ccgctggcga agatcgttag ctacggttct      840

gcgggcgttg atccggccat tatgggttat ggcccgttct acgcgaccaa agcggccatc      900

gaaaaggccg gctggacggt tgatgaactg gacctgattg aatcaaacga agcgtttgca      960

gctcagtcgc tggccgtcgc caaagacctg aagttcgata tgaacaaggt gaacgttaac     1020

ggcggtgcaa ttgctctggg tcatccgatc ggtgcatcag gcgcccgtat tctggttacc     1080

ctggtccacg cgatgcaaaa acgcgatgca aaaaagggtc tggctaccct gtgcattggc     1140

ggtggccagg gcacggcaat cctgctggaa aaatgttaa                            1179


<210>  6
<211>  392
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  6

Met Lys Glu Val Val Ile Ala Ser Ala Val Arg Thr Ala Ile Gly Ser 
1               5                   10                  15      


Tyr Gly Lys Ser Leu Lys Asp Val Pro Ala Val Asp Leu Gly Ala Thr 
            20                  25                  30          


Ala Ile Lys Glu Ala Val Lys Lys Ala Gly Ile Lys Pro Glu Asp Val 
        35                  40                  45              


Asn Glu Val Ile Leu Gly Asn Val Leu Gln Ala Gly Leu Gly Gln Asn 
    50                  55                  60                  


Pro Ala Arg Gln Ala Ser Phe Lys Ala Gly Leu Pro Val Glu Ile Pro 
65                  70                  75                  80  


Ala Met Thr Ile Asn Lys Val Cys Gly Ser Gly Leu Arg Thr Val Ser 
                85                  90                  95      


Leu Ala Ala Gln Ile Ile Lys Ala Gly Asp Ala Asp Val Ile Ile Ala 
            100                 105                 110         


Gly Gly Met Glu Asn Met Ser Arg Ala Pro Tyr Leu Ala Asn Asn Ala 
        115                 120                 125             


Arg Trp Gly Tyr Arg Met Gly Asn Ala Lys Phe Val Asp Glu Met Ile 
    130                 135                 140                 


Thr Asp Gly Leu Trp Asp Ala Phe Asn Asp Tyr His Met Gly Ile Thr 
145                 150                 155                 160 


Ala Glu Asn Ile Ala Glu Arg Trp Asn Ile Ser Arg Glu Glu Gln Asp 
                165                 170                 175     


Glu Phe Ala Leu Ala Ser Gln Lys Lys Ala Glu Glu Ala Ile Lys Ser 
            180                 185                 190         


Gly Gln Phe Lys Asp Glu Ile Val Pro Val Val Ile Lys Gly Arg Lys 
        195                 200                 205             


Gly Glu Thr Val Val Asp Thr Asp Glu His Pro Arg Phe Gly Ser Thr 
    210                 215                 220                 


Ile Glu Gly Leu Ala Lys Leu Lys Pro Ala Phe Lys Lys Asp Gly Thr 
225                 230                 235                 240 


Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Cys Ala Ala Val Leu 
                245                 250                 255     


Val Ile Met Ser Ala Glu Lys Ala Lys Glu Leu Gly Val Lys Pro Leu 
            260                 265                 270         


Ala Lys Ile Val Ser Tyr Gly Ser Ala Gly Val Asp Pro Ala Ile Met 
        275                 280                 285             


Gly Tyr Gly Pro Phe Tyr Ala Thr Lys Ala Ala Ile Glu Lys Ala Gly 
    290                 295                 300                 


Trp Thr Val Asp Glu Leu Asp Leu Ile Glu Ser Asn Glu Ala Phe Ala 
305                 310                 315                 320 


Ala Gln Ser Leu Ala Val Ala Lys Asp Leu Lys Phe Asp Met Asn Lys 
                325                 330                 335     


Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Ile Gly Ala 
            340                 345                 350         


Ser Gly Ala Arg Ile Leu Val Thr Leu Val His Ala Met Gln Lys Arg 
        355                 360                 365             


Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Gln Gly 
    370                 375                 380                 


Thr Ala Ile Leu Leu Glu Lys Cys 
385                 390         


