                         SEQUENCE LISTING

<110>  Allozyne, Inc
 
<120>  Cell lines

<130>  ALL-P1457PCT

<150>  US 61/705,116
<151>  2012-09-24

<150>  US 61/862,495
<151>  2013-08-05

<160>  84    

<170>  PatentIn version 3.3

<210>  1
<211>  454
<212>  PRT
<213>  Methanosarcina mazei

<400>  1

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 


Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  2
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: PylRS Methanosarcina mazei Y384F mutant amino
       acid sequence

<400>  2

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 


Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Phe 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  3
<211>  419
<212>  PRT
<213>  Methanosarcina barkeri

<400>  3

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His Tyr Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Gly Lys Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Pro Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asp Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Pro Val Pro Thr Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Ile Ala Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Asp Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Leu Arg Lys Leu Asp Arg Ile Leu Pro Asp Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ser Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  4
<211>  288
<212>  PRT
<213>  Desulfitobacterium hafniense

<400>  4

Met Phe Leu Thr Arg Arg Asp Pro Pro Leu Ser Ser Phe Trp Thr Lys 
1               5                   10                  15      


Val Gln Tyr Gln Arg Leu Lys Glu Leu Asn Ala Ser Gly Glu Gln Leu 
            20                  25                  30          


Glu Met Gly Phe Ser Asp Ala Leu Ser Arg Asp Arg Ala Phe Gln Gly 
        35                  40                  45              


Ile Glu His Gln Leu Met Ser Gln Gly Lys Arg His Leu Glu Gln Leu 
    50                  55                  60                  


Arg Thr Val Lys His Arg Pro Ala Leu Leu Glu Leu Glu Glu Lys Leu 
65                  70                  75                  80  


Ala Lys Ala Leu His Gln Gln Gly Phe Val Gln Val Val Thr Pro Thr 
                85                  90                  95      


Ile Ile Thr Lys Ser Ala Leu Ala Lys Met Thr Ile Gly Glu Asp His 
            100                 105                 110         


Pro Leu Phe Ser Gln Val Phe Trp Leu Asp Gly Lys Lys Cys Leu Arg 
        115                 120                 125             


Pro Met Leu Ala Pro Asn Leu Tyr Thr Leu Trp Arg Glu Leu Glu Arg 
    130                 135                 140                 


Leu Trp Asp Lys Pro Ile Arg Ile Phe Glu Ile Gly Thr Cys Tyr Arg 
145                 150                 155                 160 


Lys Glu Ser Gln Gly Ala Gln His Leu Asn Glu Phe Thr Met Leu Asn 
                165                 170                 175     


Leu Thr Glu Leu Gly Thr Pro Leu Glu Glu Arg His Gln Arg Leu Glu 
            180                 185                 190         


Asp Met Ala Arg Trp Val Leu Glu Ala Ala Gly Ile Arg Glu Phe Glu 
        195                 200                 205             


Leu Val Thr Glu Ser Ser Val Val Tyr Gly Asp Thr Val Asp Val Met 
    210                 215                 220                 


Lys Gly Asp Leu Glu Leu Ala Ser Gly Ala Met Gly Pro His Phe Leu 
225                 230                 235                 240 


Asp Glu Lys Trp Glu Ile Phe Asp Pro Trp Val Gly Leu Gly Phe Gly 
                245                 250                 255     


Leu Glu Arg Leu Leu Met Ile Arg Glu Gly Thr Gln His Val Gln Ser 
            260                 265                 270         


Met Ala Arg Ser Leu Ser Tyr Leu Asp Gly Val Arg Leu Asn Ile Asn 
        275                 280                 285             


<210>  5
<211>  443
<212>  PRT
<213>  Methanosarcina acetivorans

<400>  5

Met Asp Lys Lys Pro Leu Asp Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Met Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Glu Arg Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Arg His Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Lys Thr Ser Glu Glu Lys Thr Thr Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Arg Val Arg Lys Ala Met Pro Lys Ser Val Ala 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Ala Thr Ala Gln Val Pro Leu Ser Gly 
        115                 120                 125             


Ser Lys Pro Ala Pro Ala Thr Pro Val Ser Ala Pro Ala Gln Ala Pro 
    130                 135                 140                 


Ala Pro Ser Thr Gly Ser Ala Ser Ala Thr Ser Ala Ser Ala Gln Arg 
145                 150                 155                 160 


Met Ala Asn Ser Ala Ala Ala Pro Ala Ala Pro Val Pro Thr Ser Ala 
                165                 170                 175     


Pro Ala Leu Thr Lys Gly Gln Leu Asp Arg Leu Glu Gly Leu Leu Ser 
            180                 185                 190         


Pro Lys Asp Glu Ile Ser Leu Asp Ser Glu Lys Pro Phe Arg Glu Leu 
        195                 200                 205             


Glu Ser Glu Leu Leu Ser Arg Arg Lys Lys Asp Leu Lys Arg Ile Tyr 
    210                 215                 220                 


Ala Glu Glu Arg Glu Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr 
225                 230                 235                 240 


Lys Phe Phe Val Asp Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu 
                245                 250                 255     


Ile Pro Ala Glu Tyr Val Glu Arg Met Gly Ile Asn Ser Asp Thr Glu 
            260                 265                 270         


Leu Ser Lys Gln Val Phe Arg Ile Asp Lys Asn Phe Cys Leu Arg Pro 
        275                 280                 285             


Met Leu Ala Pro Asn Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala 
    290                 295                 300                 


Leu Pro Asp Pro Ile Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys 
305                 310                 315                 320 


Glu Ser Asp Gly Lys Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe 
                325                 330                 335     


Cys Gln Met Gly Ser Gly Cys Thr Arg Glu Asn Leu Glu Ala Ile Ile 
            340                 345                 350         


Thr Glu Phe Leu Asn His Leu Gly Ile Asp Phe Glu Ile Ile Gly Asp 
        355                 360                 365             


Ser Cys Met Val Tyr Gly Asn Thr Leu Asp Val Met His Asp Asp Leu 
    370                 375                 380                 


Glu Leu Ser Ser Ala Val Val Gly Pro Val Pro Leu Asp Arg Glu Trp 
385                 390                 395                 400 


Gly Ile Asp Lys Pro Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu 
                405                 410                 415     


Leu Lys Val Met His Gly Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser 
            420                 425                 430         


Glu Ser Tyr Tyr Asn Gly Ile Ser Thr Asn Leu 
        435                 440             


<210>  6
<211>  416
<212>  PRT
<213>  Methanococcoides  burtonii

<400>  6

Met Glu Lys Gln Leu Leu Asp Val Leu Val Glu Leu Asn Gly Val Trp 
1               5                   10                  15      


Leu Ser Arg Ser Gly Leu Leu His Gly Ile Arg Asn Phe Glu Ile Thr 
            20                  25                  30          


Thr Lys His Ile His Ile Glu Thr Asp Cys Gly Ala Arg Phe Thr Val 
        35                  40                  45              


Arg Asn Ser Arg Ser Ser Arg Ser Ala Arg Ser Leu Arg His Asn Lys 
    50                  55                  60                  


Tyr Arg Lys Pro Cys Lys Arg Cys Arg Pro Ala Asp Glu Gln Ile Asp 
65                  70                  75                  80  


Arg Phe Val Lys Lys Thr Phe Lys Glu Lys Arg Gln Thr Val Ser Val 
                85                  90                  95      


Phe Ser Ser Pro Lys Lys His Val Pro Lys Lys Pro Lys Val Ala Val 
            100                 105                 110         


Ile Lys Ser Phe Ser Ile Ser Thr Pro Ser Pro Lys Glu Ala Ser Val 
        115                 120                 125             


Ser Asn Ser Ile Pro Thr Pro Ser Ile Ser Val Val Lys Asp Glu Val 
    130                 135                 140                 


Lys Val Pro Glu Val Lys Tyr Thr Pro Ser Gln Ile Glu Arg Leu Lys 
145                 150                 155                 160 


Thr Leu Met Ser Pro Asp Asp Lys Ile Pro Ile Gln Asp Glu Leu Pro 
                165                 170                 175     


Glu Phe Lys Val Leu Glu Lys Glu Leu Ile Gln Arg Arg Arg Asp Asp 
            180                 185                 190         


Leu Lys Lys Met Tyr Glu Glu Asp Arg Glu Asp Arg Leu Gly Lys Leu 
        195                 200                 205             


Glu Arg Asp Ile Thr Glu Phe Phe Val Asp Arg Gly Phe Leu Glu Ile 
    210                 215                 220                 


Lys Ser Pro Ile Met Ile Pro Phe Glu Tyr Ile Glu Arg Met Gly Ile 
225                 230                 235                 240 


Asp Lys Asp Asp His Leu Asn Lys Gln Ile Phe Arg Val Asp Glu Ser 
                245                 250                 255     


Met Cys Leu Arg Pro Met Leu Ala Pro Cys Leu Tyr Asn Tyr Leu Arg 
            260                 265                 270         


Lys Leu Asp Lys Val Leu Pro Asp Pro Ile Arg Ile Phe Glu Ile Gly 
        275                 280                 285             


Pro Cys Tyr Arg Lys Glu Ser Asp Gly Ser Ser His Leu Glu Glu Phe 
    290                 295                 300                 


Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr Arg Glu Asn 
305                 310                 315                 320 


Met Glu Ala Leu Ile Asp Glu Phe Leu Glu His Leu Gly Ile Glu Tyr 
                325                 330                 335     


Glu Ile Glu Ala Asp Asn Cys Met Val Tyr Gly Asp Thr Ile Asp Ile 
            340                 345                 350         


Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly Pro Ile Pro 
        355                 360                 365             


Leu Asp Arg Glu Trp Gly Val Asn Lys Pro Trp Met Gly Ala Gly Phe 
    370                 375                 380                 


Gly Leu Glu Arg Leu Leu Lys Val Arg His Asn Tyr Thr Asn Ile Arg 
385                 390                 395                 400 


Arg Ala Ser Arg Ser Glu Leu Tyr Tyr Asn Gly Ile Asn Thr Asn Leu 
                405                 410                 415     


<210>  7
<211>  478
<212>  PRT
<213>  Methanosarcina thermophila

<400>  7

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Lys Leu His Lys Ile Arg His His Glu Val Ser 
            20                  25                  30          


Lys Arg Lys Ile Tyr Ile Glu Met Glu Cys Gly Glu Arg Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Ala Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Ile Cys Lys His Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Arg Thr Asn Glu Asp Lys Ser Asn Ala Lys Val Thr 
                85                  90                  95      


Val Val Ser Ala Pro Lys Ile Arg Lys Val Met Pro Lys Ser Val Ala 
            100                 105                 110         


Arg Thr Pro Lys Pro Leu Glu Asn Thr Ala Pro Val Gln Thr Leu Pro 
        115                 120                 125             


Ser Glu Ser Gln Pro Ala Pro Thr Thr Pro Ile Ser Ala Ser Thr Thr 
    130                 135                 140                 


Ala Pro Ala Ser Thr Ser Thr Thr Ala Pro Ala Pro Ala Ser Thr Thr 
145                 150                 155                 160 


Ala Pro Ala Pro Ala Ser Thr Thr Ala Pro Ala Ser Ala Ser Thr Thr 
                165                 170                 175     


Ile Ser Thr Ser Ala Met Pro Ala Ser Thr Ser Ala Gln Gly Thr Thr 
            180                 185                 190         


Lys Phe Asn Tyr Ile Ser Gly Gly Phe Pro Arg Pro Ile Pro Val Gln 
        195                 200                 205             


Ala Ser Ala Pro Ala Leu Thr Lys Ser Gln Ile Asp Arg Leu Gln Gly 
    210                 215                 220                 


Leu Leu Ser Pro Lys Asp Glu Ile Ser Leu Asp Ser Gly Thr Pro Phe 
225                 230                 235                 240 


Arg Lys Leu Glu Ser Glu Leu Leu Ser Arg Arg Arg Lys Asp Leu Lys 
                245                 250                 255     


Gln Ile Tyr Ala Glu Glu Arg Glu His Tyr Leu Gly Lys Leu Glu Arg 
            260                 265                 270         


Glu Ile Thr Lys Phe Phe Val Asp Arg Gly Phe Leu Glu Ile Lys Ser 
        275                 280                 285             


Pro Ile Leu Ile Pro Met Glu Tyr Ile Glu Arg Met Gly Ile Asp Asn 
    290                 295                 300                 


Asp Lys Glu Leu Ser Lys Gln Ile Phe Arg Val Asp Asn Asn Phe Cys 
305                 310                 315                 320 


Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr Asn Tyr Leu Arg Lys Leu 
                325                 330                 335     


Asn Arg Ala Leu Pro Asp Pro Ile Lys Ile Phe Glu Ile Gly Pro Cys 
            340                 345                 350         


Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu Glu Glu Phe Thr Met 
        355                 360                 365             


Leu Asn Phe Cys Gln Met Gly Ser Gly Cys Thr Arg Glu Asn Leu Glu 
    370                 375                 380                 


Ala Ile Ile Lys Asp Phe Leu Asp Tyr Leu Gly Ile Asp Phe Glu Ile 
385                 390                 395                 400 


Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr Leu Asp Val Met His 
                405                 410                 415     


Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly Pro Val Pro Met Asp 
            420                 425                 430         


Arg Asp Trp Gly Ile Asn Lys Pro Trp Ile Gly Ala Gly Phe Gly Leu 
        435                 440                 445             


Glu Arg Leu Leu Lys Val Met His Asn Phe Lys Asn Ile Lys Arg Ala 
    450                 455                 460                 


Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser Thr Asn Leu 
465                 470                 475             


<210>  8
<211>  427
<212>  PRT
<213>  Methanosalsum zhilinae

<400>  8

Met Thr Arg Arg Ser Leu Glu Ser Leu Val Ser Glu Lys Glu Val Trp 
1               5                   10                  15      


Leu Ser Arg Lys Gly Leu Leu His Glu Ile Lys Asp Tyr Ser Val Thr 
            20                  25                  30          


Gln Arg Tyr Ile Asn Ile Tyr Thr Thr Cys Gly Glu Ser Phe Ser Val 
        35                  40                  45              


Arg Asn Ser Arg Arg Gly Arg Ala Ser Arg Val Leu Arg Asn Asn Lys 
    50                  55                  60                  


Tyr Arg Lys Ile Cys Lys His Cys Lys Val Pro Asp Glu Lys Ile Ser 
65                  70                  75                  80  


Lys Phe Leu Gln Lys Ala Ser Val Asp Ser Thr Ala Lys Val Lys Val 
                85                  90                  95      


Val Ser Ser Thr Lys Pro Ser Gln Ser Lys Lys Ala Val Pro Lys Ala 
            100                 105                 110         


Val Lys Ala Lys Lys Lys Gly Thr Glu Asn Ser Asn Gly Ser Leu Ile 
        115                 120                 125             


Gln Ser Lys Val Lys Asp Gln Gly Ser Val Asn Ala Ile Ser Ser Gly 
    130                 135                 140                 


Gln Pro Arg Ser Lys Ile Gln Pro Thr Glu Glu Arg Asn Asn Ile Pro 
145                 150                 155                 160 


Ala Phe Thr Pro Ser Gln Lys Lys Arg Leu Glu Ala Leu Leu Met Pro 
                165                 170                 175     


Glu Glu Val Ile Pro Asp Pro Ser Glu Asn Leu Asn Phe Gln Glu Leu 
            180                 185                 190         


Glu Ser Ser Leu Val Asn Arg Arg Lys Lys Asp Ile Val Lys Ile Tyr 
        195                 200                 205             


Glu Asp Asp Arg Glu Asn Gln Leu Gly Lys Ile Glu Arg Ile Ile Thr 
    210                 215                 220                 


Lys Phe Phe Val Asp Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu 
225                 230                 235                 240 


Ile Pro Ile Glu Tyr Ile Glu Arg Met Gly Ile Thr Glu Asp Lys Glu 
                245                 250                 255     


Leu Phe Glu Gln Val Phe Lys Val Asp Lys Asn Met Cys Leu Arg Pro 
            260                 265                 270         


Met Leu Ala Pro Gly Leu Tyr Asn His Leu Arg Lys Phe Asp Lys Val 
        275                 280                 285             


Leu Pro Asp Pro Ile Arg Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys 
    290                 295                 300                 


Glu Ser Asp Gly Ser Gln His Leu Glu Glu Phe Thr Met Leu Asn Phe 
305                 310                 315                 320 


Cys Gln Met Gly Ser Met Cys Thr Arg Lys Thr Leu Glu Asn Leu Ile 
                325                 330                 335     


Asp Glu Leu Leu Glu Phe Met Asp Ile Glu Tyr Glu Ile Val Ser Asp 
            340                 345                 350         


Asn Cys His Val Tyr Gly Ala Thr Ile Asp Val Leu His Lys Asp Met 
        355                 360                 365             


Glu Leu Ala Ser Ala Val Val Gly Pro Ile Pro Lys Asp Ala Asp Trp 
    370                 375                 380                 


Gly Ile Thr Lys Pro Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu 
385                 390                 395                 400 


Leu Lys Val Met His Asn Tyr Lys Asn Ile Arg Arg Ala Ser Arg Ser 
                405                 410                 415     


Glu Ser Tyr Tyr Asn Gly Ile Thr Thr Asn Leu 
            420                 425         


<210>  9
<211>  414
<212>  PRT
<213>  Methanohalobium evestigatum

<400>  9

Met Ser Lys Lys Ser Leu Ala Ser Leu Ile Ser Asp Leu Gln Val Trp 
1               5                   10                  15      


Val Ser Arg Ser Gly Leu Leu His Glu Ile Lys Asn Tyr Glu Val Ser 
            20                  25                  30          


Gln Arg Tyr Ile His Met Glu Met Asp Cys Gly Glu Lys Ile Thr Val 
        35                  40                  45              


Arg Asn Ser Arg Asn Ser Arg Thr Ala Arg Ile Leu Arg Leu Lys Lys 
    50                  55                  60                  


Tyr Lys Lys Pro Cys Lys Asn Cys Lys Val Ser Asp Glu Val Ile Asn 
65                  70                  75                  80  


Arg Phe Leu Gln Lys His Thr Asp Arg Thr Asp Thr Lys Val Thr Ala 
                85                  90                  95      


Phe Ser Tyr Ser Glu Ser Lys Lys Lys Lys Ser Lys Gln Leu Gly His 
            100                 105                 110         


Lys Lys Lys Lys Gln Ser Lys Val Gln Val Asn Pro Thr Thr Glu Ser 
        115                 120                 125             


Ile Gln Ser Asn Thr Ser Val Ser Glu Asp Lys Thr Asp Asn Lys Ile 
    130                 135                 140                 


Glu Pro Glu Thr Phe Thr Ser Ala Gln Lys Glu Arg Ile Asn Glu Leu 
145                 150                 155                 160 


Leu Leu Pro Gly Glu Lys Ile Pro Phe Ser Asn Glu Pro Ser Lys Phe 
                165                 170                 175     


Lys Glu Ile Glu Ser Glu Leu Val Asn Lys Arg Arg Asn Asp Phe Lys 
            180                 185                 190         


Gln Met Tyr Glu Asn Asp Arg Glu Glu Gln Ile Ala Lys Leu Glu Arg 
        195                 200                 205             


Thr Ile Ser Gln Phe Phe Val Asp Lys Gly Phe Ile Glu Ile Lys Ala 
    210                 215                 220                 


Pro Ile Ile Ile Asp Ile Asp Ser Val Lys Lys Met Gly Ile Asp Thr 
225                 230                 235                 240 


Asp His Lys Leu Ser Lys Gln Ile Phe Tyr Leu Asp Asn Lys His Cys 
                245                 250                 255     


Leu Arg Pro Met Leu Ala Pro Gly Leu Tyr Gln Trp Leu Lys Asn Phe 
            260                 265                 270         


Asp Lys Ile Leu Pro Asp Pro Ile Lys Ile Phe Glu Ile Gly Pro Cys 
        275                 280                 285             


Tyr Arg Lys Glu Ser Glu Gly Ser Gln His Leu Glu Glu Phe Thr Met 
    290                 295                 300                 


Phe Asn Phe Cys Gln Met Gly Ser Gly Ala Asn Arg Glu Asn Leu Leu 
305                 310                 315                 320 


Asn His Ile Asp Asp Leu Leu Lys His Leu Asn Ile Asp Tyr Lys Ile 
                325                 330                 335     


Ile Asp Asp Asn Cys His Val Tyr Gly Glu Thr Ile Asp Ile Val His 
            340                 345                 350         


Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly Pro Val Pro Ile Asp 
        355                 360                 365             


Met Asn Trp Gly Ile Asp Lys Thr Trp Ile Gly Ala Gly Leu Gly Leu 
    370                 375                 380                 


Glu Arg Leu Leu Lys Val Lys His Gly Tyr Lys Asn Ile Lys Arg Ala 
385                 390                 395                 400 


Ser Lys Ser His Ser Tyr Tyr Asn Gly Ile Ser Thr Asn Leu 
                405                 410                 


<210>  10
<211>  404
<212>  PRT
<213>  Methanohalophilus mahii

<400>  10

Met Glu Arg Lys Pro Leu Asp Leu Leu Ile Asp Thr Asn Gly Val Trp 
1               5                   10                  15      


Leu Ser Arg Asn Gly Leu Leu His Gly Val Lys Asn Phe Glu Val Ser 
            20                  25                  30          


Arg Asn His Ile His Ile Thr Thr Asp Cys Gln Ser Arg Phe Thr Val 
        35                  40                  45              


Arg Asn Ser Arg Arg Ser Arg Ser Ala Arg Ala Leu Arg Asn Asn Lys 
    50                  55                  60                  


Tyr Arg Lys Ala Cys Lys Asn Cys Lys Leu Ser Asp Glu Arg Ile Thr 
65                  70                  75                  80  


Arg Phe Val Thr Lys Asp Phe Gly Arg Gly Ser Gln Ala Arg Val Ile 
                85                  90                  95      


Thr Ser Ser Lys Thr Lys Lys Ser Lys Ser Pro Lys Glu Ala Val Val 
            100                 105                 110         


Lys Ser Val Ser Ser Lys Ala Asn Glu Met Pro Pro Val Val Glu Ala 
        115                 120                 125             


Lys Lys Glu Lys Pro Val Lys Pro Asp Tyr Thr Pro Ala Gln Lys Lys 
    130                 135                 140                 


Arg Ile Thr Thr Leu Leu Ser Pro Ala Asp Asp Leu Ser Ser Ile Lys 
145                 150                 155                 160 


Glu Leu Pro Thr Phe Lys Glu Leu Glu Thr Glu Leu Val Lys Lys Arg 
                165                 170                 175     


Lys Gln Asp Leu Arg Gln Met Tyr Glu Asp Asp Arg Arg His Gln Leu 
            180                 185                 190         


Ala Gln Leu Glu Arg Asp Ile Ser Leu Phe Leu Ile Glu Lys Gly Phe 
        195                 200                 205             


Met Glu Val Arg Thr Ser Val Leu Ile Pro Ala Lys Phe Ile Glu Arg 
    210                 215                 220                 


Met Gly Ile Thr Glu Glu Asp Pro Leu Tyr Lys Gln Ile Phe Arg Val 
225                 230                 235                 240 


Asp Glu Asn Thr Cys Leu Arg Pro Met Leu Ala Pro Gly Leu Tyr Asn 
                245                 250                 255     


Tyr Leu His Asn Phe Asp Asn Ile Met Pro Asp Pro Leu Lys Ile Phe 
            260                 265                 270         


Glu Ile Gly Thr Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
        275                 280                 285             


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
    290                 295                 300                 


Lys Glu Asn Leu Leu Asn Ile Ile Asp Asp Leu Leu Lys Tyr Leu Asn 
305                 310                 315                 320 


Ile Asp Tyr Glu Val Ile Ser Asp Asn Cys Met Val Tyr Gly Asp Thr 
                325                 330                 335     


Ile Asp Ile Met His Gly Asp Met Glu Ile Ser Ser Ala Val Val Gly 
            340                 345                 350         


Pro Ile Pro Gln Asp Leu Asp Trp Gly Val Thr Lys Pro Trp Met Gly 
        355                 360                 365             


Ala Gly Met Gly Ile Glu Arg Leu Leu Lys Val Lys His Lys Tyr Thr 
    370                 375                 380                 


Asn Ile Lys Arg Ser Ser Arg Ser Ile Ser Tyr Tyr Asn Gly Ile Thr 
385                 390                 395                 400 


Thr Asn Leu Arg 
                


<210>  11
<211>  277
<212>  PRT
<213>  Desulfotomaculum gibsoniae

<400>  11

Met Ile Asn Trp Ser Ile Ser Gln Lys Gln Lys Leu Ile Glu Leu Asn 
1               5                   10                  15      


Gly Lys Ser Glu Leu Leu Asp Leu Arg Phe Gln Asp Lys Gln Lys Arg 
            20                  25                  30          


Asp Gln Thr Phe Gln Lys Ile Glu Lys Glu Leu Val Lys Lys Asn Lys 
        35                  40                  45              


Asp His Leu Leu Glu Leu Lys Glu Val Ile His Arg Pro Leu Leu Ser 
    50                  55                  60                  


Ser Leu Glu Ile Gln Leu Ser Asn Leu Leu Cys Lys Thr Gly Phe Val 
65                  70                  75                  80  


Gln Val Asn Thr Pro Ile Ile Leu Pro Lys Ala Met Leu His Lys Met 
                85                  90                  95      


Thr Ile Thr Pro Glu His Pro Leu Tyr Lys Gln Val Phe Trp Val Asp 
            100                 105                 110         


Asn Asn Lys Cys Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr His Tyr 
        115                 120                 125             


Leu Lys Ile Leu Asp Arg Leu Trp Ser Lys Pro Val Arg Ile Phe Glu 
    130                 135                 140                 


Ile Gly Pro Cys Phe Arg Lys Glu Ser Gln Gly Ala Gln His Leu Asn 
145                 150                 155                 160 


Glu Phe Thr Met Leu Asn Leu Val Glu Leu Gly Val Asp Lys Gly Lys 
                165                 170                 175     


Gln Thr Glu Arg Leu Lys Glu Leu Gly Ser Leu Val Met Glu Glu Ile 
            180                 185                 190         


Gly Val Lys Asn Tyr Glu Phe Val Glu Thr Glu Ser Glu Ile Tyr Gly 
        195                 200                 205             


Ile Thr Val Asp Val Val Phe Asp Asp Leu Glu Leu Gly Ser Gly Ala 
    210                 215                 220                 


Phe Gly Pro Leu Lys Met Asp Glu Gln Trp Gly Ile Phe Glu Pro Trp 
225                 230                 235                 240 


Val Gly Ile Gly Phe Gly Leu Glu Arg Leu Ala Met Thr Leu Gln Gly 
                245                 250                 255     


His Arg Asn Ile Arg Arg Val Gly Arg Gly Leu Thr Tyr Leu Asp Gly 
            260                 265                 270         


Ser Leu Leu Asn Ile 
        275         


<210>  12
<211>  277
<212>  PRT
<213>  Desulfosporosinus meridiei

<400>  12

Met Ser Ile Thr Trp Thr Pro Ile Gln Lys Gln Arg Leu Gln Glu Leu 
1               5                   10                  15      


Asn Ala Ser Glu Val Leu Gln Glu Met Cys Phe Glu Ser His Gln Glu 
            20                  25                  30          


Arg Asp Arg Ala Tyr Gln Glu Gln Glu His Leu Leu Val Ile Arg Gly 
        35                  40                  45              


Lys Gln Lys Leu Gln Glu Leu Leu Glu Thr Asn Arg Arg Pro Ser Leu 
    50                  55                  60                  


Ser Val Leu Glu Gln Gln Leu Val Glu Ala Leu Thr Gln Glu Gly Phe 
65                  70                  75                  80  


Val Gln Val Val Thr Pro Thr Ile Ile Ser Lys Thr Ala Leu Ala Lys 
                85                  90                  95      


Met Ser Val Ser Asp Asp His Pro Leu Phe Ser Gln Val Phe Trp Leu 
            100                 105                 110         


Asp Ser Lys Arg Cys Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr Thr 
        115                 120                 125             


Leu Trp Arg Asp Leu Leu Arg Leu Trp Glu Lys Pro Ile Arg Ile Phe 
    130                 135                 140                 


Glu Ile Gly Thr Cys Tyr Arg Lys Glu Ser Lys Gly Ser Leu His Leu 
145                 150                 155                 160 


Asn Glu Phe Thr Met Leu Asn Leu Thr Glu Leu Gly Leu Pro Leu Asp 
                165                 170                 175     


Gln Arg His Gln Arg Leu Lys Glu Leu Ala Asp Leu Val Met Asn Thr 
            180                 185                 190         


Val Gly Ile Asp Asp Tyr Gln Leu Glu Ser Glu Ser Ser Val Val Tyr 
        195                 200                 205             


Gly Asp Thr Leu Asp Val Val Lys Gly Ile Glu Leu Gly Ser Ser Ala 
    210                 215                 220                 


Met Gly Pro His Val Leu Asp Asp Gln Trp Gly Ile Phe Asp Pro Trp 
225                 230                 235                 240 


Val Gly Ile Gly Phe Gly Leu Glu Arg Leu Leu Met Ile Lys Glu Gly 
                245                 250                 255     


Ser Gln Asn Val Gln Ser Met Gly Arg Ser Leu Thr Tyr Leu Asn Gly 
            260                 265                 270         


Val Arg Leu Asn Ile 
        275         


<210>  13
<211>  278
<212>  PRT
<213>  Desulfotomaculum acetoxidans

<400>  13

Met Ser Phe Leu Trp Thr Val Ser Gln Gln Lys Arg Leu Ser Glu Leu 
1               5                   10                  15      


Asn Ala Ser Glu Glu Glu Lys Asn Met Ser Phe Ser Ser Thr Ser Asp 
            20                  25                  30          


Arg Glu Ala Ala Tyr Lys Arg Val Glu Met Arg Leu Ile Asn Glu Ser 
        35                  40                  45              


Lys Gln Arg Leu Asn Lys Leu Arg His Glu Thr Lys Arg Pro Ala Ile 
    50                  55                  60                  


Cys Ala Leu Glu Asn Arg Leu Ala Ala Ala Leu Arg Gly Ala Gly Phe 
65                  70                  75                  80  


Val Gln Val Ala Thr Pro Val Ile Leu Ser Lys Lys Leu Leu Gly Lys 
                85                  90                  95      


Met Thr Ile Thr Asp Glu His Ala Leu Phe Ser Gln Val Phe Trp Ile 
            100                 105                 110         


Glu Glu Asn Lys Cys Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr Tyr 
        115                 120                 125             


Ile Leu Lys Asp Leu Leu Arg Leu Trp Glu Lys Pro Val Arg Ile Phe 
    130                 135                 140                 


Glu Ile Gly Ser Cys Phe Arg Lys Glu Ser Gln Gly Ser Asn His Leu 
145                 150                 155                 160 


Asn Glu Phe Thr Met Leu Asn Leu Val Glu Trp Gly Leu Pro Glu Glu 
                165                 170                 175     


Gln Arg Gln Lys Arg Ile Ser Glu Leu Ala Lys Leu Val Met Asp Glu 
            180                 185                 190         


Thr Gly Ile Asp Glu Tyr His Leu Glu His Ala Glu Ser Val Val Tyr 
        195                 200                 205             


Gly Glu Thr Val Asp Val Met His Arg Asp Ile Glu Leu Gly Ser Gly 
    210                 215                 220                 


Ala Leu Gly Pro His Phe Leu Asp Gly Arg Trp Gly Val Val Gly Pro 
225                 230                 235                 240 


Trp Val Gly Ile Gly Phe Gly Leu Glu Arg Leu Leu Met Val Glu Gln 
                245                 250                 255     


Gly Gly Gln Asn Val Arg Ser Met Gly Lys Ser Leu Thr Tyr Leu Asp 
            260                 265                 270         


Gly Val Arg Leu Asn Ile 
        275             


<210>  14
<211>  1365
<212>  DNA
<213>  Methanosarcina mazei

<400>  14
atggataaaa aaccactaaa cactctgata tctgcaaccg ggctctggat gtccaggacc     60

ggaacaattc ataaaataaa acaccacgaa gtctctcgaa gcaaaatcta tattgaaatg    120

gcatgcggag accaccttgt tgtaaacaac tccaggagca gcaggactgc aagagcgctc    180

aggcaccaca aatacaggaa gacctgcaaa cgctgcaggg tttcggatga ggatctcaat    240

aagttcctca caaaggcaaa cgaagaccag acaagcgtaa aagtcaaggt cgtttctgcc    300

cctaccagaa cgaaaaaggc aatgccaaaa tccgttgcga gagccccgaa acctcttgag    360

aatacagaag cggcacaggc tcaaccttct ggatctaaat tttcacctgc gataccggtt    420

tccacccaag agtcagtttc tgtcccggca tctgtttcaa catcaatatc aagcatttct    480

acaggagcaa ctgcatccgc actggtaaaa gggaatacga accccattac atccatgtct    540

gcccctgttc aggcaagtgc ccccgcactt acgaagagcc agactgacag gcttgaagtc    600

ctgttaaacc caaaagatga gatttccctg aattccggca agcctttcag ggagcttgag    660

tccgaattgc tctctcgcag aaaaaaagac ctgcagcaga tctacgcgga agaaagggag    720

aattatctgg ggaaactcga gcgtgaaatt accaggttct ttgtggacag gggttttctg    780

gaaataaaat ccccgatcct gatccctctt gagtatatcg aaaggatggg cattgataat    840

gataccgaac tttcaaaaca gatcttcagg gttgacaaga acttctgcct gagacccatg    900

cttgctccaa acctttacaa ctacctgcgc aagcttgaca gggccctgcc tgatccaata    960

aaaatttttg aaataggccc atgctacaga aaagagtccg acggcaaaga acacctcgaa   1020

gagtttacca tgctgaactt ctgccagatg ggatcgggat gcacacggga aaatcttgaa   1080

agcataatta cggacttcct gaaccacctg ggaattgatt tcaagatcgt aggcgattcc   1140

tgcatggtct atggggatac ccttgatgta atgcacggag acctggaact ttcctctgca   1200

gtagtcggac ccataccgct tgaccgggaa tggggtattg ataaaccctg gataggggca   1260

ggtttcgggc tcgaacgcct tctaaaggtt aaacacgact ttaaaaatat caagagagct   1320

gcaaggtccg agtcttacta taacgggatt tctaccaacc tgtaa                   1365


<210>  15
<211>  1365
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: PylRS Methanosarcina mazei Y384F mutant 
       nucleotide sequence

<400>  15
atggataaaa aaccactaaa cactctgata tctgcaaccg ggctctggat gtccaggacc     60

ggaacaattc ataaaataaa acaccacgaa gtctctcgaa gcaaaatcta tattgaaatg    120

gcatgcggag accaccttgt tgtaaacaac tccaggagca gcaggactgc aagagcgctc    180

aggcaccaca aatacaggaa gacctgcaaa cgctgcaggg tttcggatga ggatctcaat    240

aagttcctca caaaggcaaa cgaagaccag acaagcgtaa aagtcaaggt cgtttctgcc    300

cctaccagaa cgaaaaaggc aatgccaaaa tccgttgcga gagccccgaa acctcttgag    360

aatacagaag cggcacaggc tcaaccttct ggatctaaat tttcacctgc gataccggtt    420

tccacccaag agtcagtttc tgtcccggca tctgtttcaa catcaatatc aagcatttct    480

acaggagcaa ctgcatccgc actggtaaaa gggaatacga accccattac atccatgtct    540

gcccctgttc aggcaagtgc ccccgcactt acgaagagcc agactgacag gcttgaagtc    600

ctgttaaacc caaaagatga gatttccctg aattccggca agcctttcag ggagcttgag    660

tccgaattgc tctctcgcag aaaaaaagac ctgcagcaga tctacgcgga agaaagggag    720

aattatctgg ggaaactcga gcgtgaaatt accaggttct ttgtggacag gggttttctg    780

gaaataaaat ccccgatcct gatccctctt gagtatatcg aaaggatggg cattgataat    840

gataccgaac tttcaaaaca gatcttcagg gttgacaaga acttctgcct gagacccatg    900

cttgctccaa acctttacaa ctacctgcgc aagcttgaca gggccctgcc tgatccaata    960

aaaatttttg aaataggccc atgctacaga aaagagtccg acggcaaaga acacctcgaa   1020

gagtttacca tgctgaactt ctgccagatg ggatcgggat gcacacggga aaatcttgaa   1080

agcataatta cggacttcct gaaccacctg ggaattgatt tcaagatcgt aggcgattcc   1140

tgcatggtct ttggggatac ccttgatgta atgcacggag acctggaact ttcctctgca   1200

gtagtcggac ccataccgct tgaccgggaa tggggtattg ataaaccctg gataggggca   1260

ggtttcgggc tcgaacgcct tctaaaggtt aaacacgact ttaaaaatat caagagagct   1320

gcaaggtccg agtcttacta taacgggatt tctaccaacc tgtaa                   1365


<210>  16
<211>  1365
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: PylRS Codon optimized Methanosarcina mazei 
       nucleotide sequence

<400>  16
atggataaaa aaccattgaa tacgctcatt agcgcaactg ggctgtggat gagccgtacg     60

ggaacgattc ataaaatcaa gcaccacgaa gtatctcgta gcaaaatcta tatcgagatg    120

gcttgcggcg accatctcgt ggtaaacaat agcaggtcct cacggaccgc ccgtgccttg    180

cgccaccaca aatatcgtaa aacttgtaag agatgtagag tgagcgacga ggatctgaac    240

aagtttctta caaaggccaa cgaggaccaa accagcgtca aagtcaaggt tgtgagcgcc    300

ccaacacgca ccaagaaggc catgcccaag tctgttgcgc gggcaccgaa acctctggag    360

aatactgagg ccgctcaggc ccagcccagc ggttcaaaat tctctcctgc cattccagtt    420

agcactcaag agtcagtcag cgtgcccgcc tctgtgtcta catccatcag ctctatctcc    480

accggcgcaa cagcctctgc cctggtgaag ggtaatacga accctatcac gagtatgtcc    540

gcacccgtgc aagcaagtgc tcccgcactc actaaatccc aaacggaccg gctggaggtc    600

ctgcttaacc ctaaggatga aatcagcctg aacagtggaa aaccgtttcg agaactggaa    660

tccgagctct taagccggcg aaagaaagat ttgcaacaga tttacgccga agaacgggaa    720

aattatctgg gcaagctgga gagagaaatc actaggttct ttgtagatag gggctttctg    780

gagattaaga gtcccatatt gatccctctc gaatacattg agcgtatggg catcgacaac    840

gacacagaac ttagcaagca gatctttcgg gtggacaaaa acttctgcct caggcctatg    900

ctggctccaa atctgtacaa ctatcttagg aaactcgacc gggccctgcc cgatcccatt    960

aaaatcttcg aaattggacc ttgctataga aaggagagcg atggcaagga gcacctggag   1020

gagtttacta tgctcaattt ctgtcaaatg ggctccggct gcacacgtga gaacctcgaa   1080

tccattataa ccgacttcct gaatcacctg gggattgatt tcaagatcgt gggcgactcc   1140

tgcatggtgt atggtgatac gttggatgtg atgcacggag atttggaatt gtcaagcgct   1200

gtggtaggcc ccattcctct cgacagggag tggggtattg acaagccctg gatcggcgca   1260

ggttttggac tggagcgcct gttgaaggtt aagcatgact tcaaaaacat aaagagagcc   1320

gcacgcagcg aatcctatta taatggaatc agcactaact tgtaa                   1365


<210>  17
<211>  1260
<212>  DNA
<213>  Methanosarcina barkeri

<400>  17
atggataaaa aaccattaga tgttttaata tctgcgaccg ggctctggat gtccaggact     60

ggcacgctcc acaaaatcaa acactatgag gtctcaagaa gtaaaatata cattgaaatg    120

gcgtgtggag accatcttgt tgtgaataat tctaggagtt gtagaacagc cagagcattc    180

agacatcata agtacagaaa aacctgcaaa cgatgtaggg tttcggacga ggatatcaat    240

aatttcctca caagatcaac tgaaggcaaa accagtgtga aagttaaggt agtttctgct    300

ccaaaggtca aaaaagctat gccgaaatca gtttcgaggg ctccaaagcc tctggaaaat    360

cctgtgtctg caaaggcatc aacggacaca tccagatctg taccttcgcc tgcaaaatca    420

actccaaatt cgcctgttcc cacatcggct cctgctcctt cacttacaag aagccagctc    480

gatagggttg aggctctctt aagtccagag gataaaattt ctctgaatat tgcaaagcct    540

ttcagggaac ttgagtccga acttgtgaca agaagaaaaa acgattttca gcggctctat    600

accaatgata gagaagacta ccttggtaaa ctcgaacggg acattacgaa atttttcgta    660

gaccgggatt ttctggagat aaagtctcct atccttattc cggcagaata cgtggagaga    720

atgggtatta acaatgatac tgaactttca aaacagatct tcagggtgga taaaaatctc    780

tgcttaaggc caatgcttgc cccgactctt tacaactatc tgcgaaaact cgataggatt    840

ttaccagatc ctataaagat tttcgaagtc gggccctgtt accggaaaga gtctgacggc    900

aaagagcacc tggaagaatt taccatggtg aacttctgtc agatgggttc gggatgtact    960

cgggaaaatc ttgaatccct catcaaagag tttctggact atctggaaat cgacttcgaa   1020

atcgtaggag attcctgtat ggtctatggg gatacccttg atataatgca cggggacctg   1080

gagctttctt cggcagtcgt cgggccagtt cctcttgata gggaatgggg cattgacaaa   1140

ccatggatag gtgcaggttt tgggcttgaa cgcttgctca aggttatgca tggctttaaa   1200

aacattaaga gagcatcaag gtccgaatct tactataatg ggatttcaac caatctatga   1260


<210>  18
<211>  867
<212>  DNA
<213>  Desulfitobacterium hafniense

<400>  18
atgtttttaa caaggaggga cccacccttg agcagctttt ggacaaaggt tcaatatcaa     60

cgcctgaaag aactcaatgc ttccggggag cagctggaaa tgggtttttc cgatgcacta    120

agccgtgacc gcgcttttca ggggattgaa catcaactga tgagccaggg aaaacgccat    180

ttggaacagc tgcgcacggt gaagcatcgt cccgccttgc tcgagcttga agagggatta    240

gcgaaggcat tgcaccaaca gggatttgtt caggtggtga ccccgacgat tattacgaag    300

tcggccttgg ctaagatgac cataggggag gaccatcctt tgttttccca ggttttttgg    360

ttggatggga agaaatgttt gcggccgatg ctggctccca atctatacac tttgtggaga    420

gagcttgagc gcctgtggga taagccgatc cggattttcg agattggaac ctgttaccgg    480

aaagagtccc agggggcaca acatctcaat gaatttacca tgctgaatct cacagaactg    540

gggactccgc tggaagagcg gcatcaacgt cttgaagaca tggcccgttg ggtgctggag    600

gctgcgggaa taagggagtt tgagctggtt acggaatcct cggtagttta cggggatacg    660

gtagatgtga tgaagggcga tctggagctg gcttcggggg ccatggggcc ccacttcctt    720

gatgaaaaat gggagatagt tgatccctgg gtaggcctgg gctttggtct ggaaaggctt    780

ctgatgattc gcgaaggaac acaacatgtt cagagtatgg ccagaagcct gagctatctt    840

gatggagtac gcttaaatat caattga                                        867


<210>  19
<211>  1332
<212>  DNA
<213>  Methanosarcina acetivorans

<400>  19
atggataaaa aaccgctaga cactctgata tctgcaaccg ggctctggat gtccaggacc     60

ggaatgattc acaaaatcaa gcaccatgaa gtttcaagaa gcaagatcta tatcgaaatg    120

gcatgtggag aaaggctcgt tgtaaataac tcccggagca gcaggactgc aagagctctc    180

aggcaccaca aatacagaaa gacctgcaga cactgcaggg tttcggacga ggatattaac    240

aacttcctca caaagaccag cgaagagaaa accaccgtga aagtcaaggt tgtttctgct    300

cccagagtca ggaaagccat gccgaaatcc gttgccagag ctccaaaacc gcttgaagcc    360

acagcacagg ttccgctttc cggatcaaaa cctgcacccg caaccccggt ttccgcacct    420

gcacaggctc ccgcaccatc aacaggctcg gcttcggcaa catctgcatc agcacagaga    480

atggcaaact ctgctgcggc tccggctgct cctgttccga cgagtgctcc tgcacttaca    540

aagggccagc ttgacaggct tgaagggctg ttaagtccta aagatgagat ttccctggat    600

tccgaaaagc ctttcaggga gcttgagtcc gaactgctct cccggagaaa aaaagacctg    660

aagcggatct atgccgaaga gagagaaaac tatctgggaa agctcgaacg cgaaattacg    720

aaattctttg tggatagggg ttttctggag atcaagtccc cgattctgat ccctgcggaa    780

tacgtggaga gaatgggcat taacagtgac acggagcttt caaagcaggt ctttagaatc    840

gacaaaaatt tctgtttaag gccaatgtta gccccgaacc tctacaacta cctgcgcaag    900

cttgacagag ccctgcctga cccgataaaa atcttcgaaa tcgggccctg ttacaggaaa    960

gaatcggacg gcaaagaaca cctggaagag tttaccatgc tgaacttctg ccagatgggg   1020

tcaggctgca cacgagaaaa ccttgaagcc ataatcacgg agttcctgaa ccacctgggg   1080

atcgactttg agattatagg ggattcctgc atggtctacg gaaataccct tgatgtcatg   1140

cacgacgacc ttgaactttc ttctgcggtc gtagggcccg tccctcttga ccgggaatgg   1200

ggaattgaca agccctggat aggagccggt ttcgggcttg aacgcctgct gaaagtcatg   1260

cacggcttca aaaacatcaa aagagctgca aggtccgaat cttactataa cgggatttcc   1320

acaaacctgt aa                                                       1332


<210>  20
<211>  1251
<212>  DNA
<213>  Methanococcoides  burtonii

<400>  20
atggaaaagc aattactgga cgttctagta gagctcaacg gagtatggct atcgcgaagc     60

ggattgcttc atgggataag gaactttgag ataaccacta aacatattca cattgagacc    120

gactgtggtg caaggttcac tgtgagaaat tccagatcaa gccgttctgc aagatcatta    180

aggcataaca aatatcgcaa accctgcaaa cgctgtcgtc cggcagatga gcagatcgac    240

cgttttgtta agaagacctt taaagagaaa aggcagactg tcagcgtatt ctccagtccg    300

aagaaacatg tcccgaaaaa gccaaaagtg gctgtcatta aatccttttc tatttctaca    360

cccagcccta aggaggcaag tgtgtcaaac tcaataccaa ctccttctat aagtgtcgta    420

aaagatgaag tgaaagtacc agaagtaaag tacacacctt cacagatcga gagactaaag    480

accctgatgt cccctgatga caagatacca atccaggacg agttacctga gttcaaggtg    540

cttgagaaag aactgataca gaggcgacgt gacgacctta agaagatgta cgaggaagac    600

agagaagacc gtcttggtaa acttgagagg gacataaccg aattcttcgt ggacagagga    660

ttcctggaaa taaaatcacc tataatgata cctttcgaat atatcgaaag gatggggata    720

gacaaggatg atcacctcaa caagcagata ttccgtgttg atgagagcat gtgtctgcga    780

cctatgctgg caccatgcct ttacaattat ctgcgcaaac ttgacaaggt gcttccggac    840

cccataagaa tattcgagat aggaccatgc taccgcaaag aatcagatgg tagcagccat    900

cttgaagagt ttaccatggt gaacttctgc cagatgggat caggatgtac aagagagaac    960

atggaagcgc tcatagatga gttccttgag catcttggaa tagaatacga gatagaggct   1020

gacaactgca tggtctatgg agataccatt gacatcatgc atggcgacct tgaactgtca   1080

tccgcagtgg tagggccaat cccgcttgac agagaatggg gtgtgaataa accgtggatg   1140

ggagcaggat tcggtcttga aagactacta aaggtcaggc acaattacac gaacatacgc   1200

cgtgcaagcc gttctgaact atactacaac ggaatcaata cgaatctatg a            1251


<210>  21
<211>  1437
<212>  DNA
<213>  Methanosarcina thermophila

<400>  21
atggataaaa aaccactaaa cactctgata tctgctacag ggctctggat gtccaggacc     60

ggaaagcttc acaaaatcag gcatcatgag gtatcaaaaa ggaaaatcta catcgaaatg    120

gaatgtggag aacggcttgt tgtgaacaat tcccggagct gcagggcagc acgggccctg    180

aggcaccata agtacaggaa gatctgcaaa cactgcaggg tttcagatga ggacctaaac    240

aagttcctca caagaacaaa cgaagacaaa agcaacgcga aagtcacggt agtttctgct    300

ccaaaaataa gaaaagtaat gccaaaatca gttgcaagaa ccccaaaacc acttgaaaat    360

acggcaccgg tacagactct gccttctgag tctcaacctg cacccactac accaatttct    420

gcatcaacaa cagcacctgc atcaacttct acaacagcac ctgcaccagc ttctacaaca    480

gcacctgcac cagcttctac aacagcacca gcttctgcat caacaacaat ctccacttcg    540

gcaatgcctg catctacatc agcacaggga acaacaaaat tcaactacat ctccggcggc    600

ttccccagac ccatccctgt tcaggcgagt gctcctgcac ttacaaagag ccagattgac    660

aggcttcaag gtctgttaag cccgaaggat gagatttccc tggattccgg aacgcccttc    720

aggaaacttg agtccgaact gctgtccagg agaagaaagg acctgaagca gatctatgcc    780

gaagaaaggg aacattacct gggaaaactc gaacgcgaga ttacgaaatt ttttgttgat    840

cggggttttc tggagataaa gtccccgatt ctgatcccta tggagtatat tgaaaggatg    900

ggcattgata acgataagga actctcaaag cagatcttca gggttgataa taacttctgc    960

ttaaggccca tgcttgctcc gaatctctac aactacctgc gcaagcttaa tagagccctg   1020

cctgacccga taaaaatctt cgagatcggg ccctgttaca ggaaagagtc tgacggcaaa   1080

gaacacctgg aagagtttac catgctgaat ttctgccaga tggggtcagg ctgcacgcgc   1140

gaaaaccttg aagccataat taaagatttc ctggactacc tggggatcga cttcgaaatt   1200

gtaggagact cctgtatggt ctacggggat acccttgatg tcatgcacgg agacctggaa   1260

ctttcctcag cagttgtcgg gcccgtgccc atggaccggg actggggaat aaacaagccc   1320

tggatagggg caggtttcgg gctcgaacgc ctgttgaagg ttatgcacaa tttcaagaat   1380

attaaaagag cttcaagatc cgaatcatac tacaacggga tttccaccaa tctgtaa      1437


<210>  22
<211>  1284
<212>  DNA
<213>  Methanosalum zhilinae

<400>  22
atgacaagaa ggtcccttga atctctggta tccgaaaaag aagtctggtt atccagaaaa     60

ggtcttcttc atgaaattaa agattacagt gttacacaga gatatattaa tatctatact    120

acctgcgggg aatcattttc tgtaagaaac tcaagaagag ggagagcttc aagggtttta    180

agaaataaca aatataggaa aatctgtaaa cattgtaaag tacctgatga aaaaatatct    240

aaattcttac agaaggcaag tgtggattct actgcaaaag taaaagtggt ttcatctaca    300

aaaccatctc aatctaagaa agcggtccct aaggctgtaa aagcgaaaaa aaagggaaca    360

gaaaacagta atggatctct tatacaatca aaagttaaag atcaagggtc agtcaatgct    420

atatcatcag gacagcctcg atcaaaaatt cagccaacgg aagaaagaaa taatattcct    480

gcattcacac ccagtcagaa aaaaaggctc gaagcactcc ttatgcctga ggaagtcatt    540

cctgatccat ccgaaaatct caatttccag gaacttgaat caagtctggt caaccgaaga    600

aaaaaagata ttgtaaaaat ctatgaggat gacagagaaa atcaattggg taaaattgaa    660

agaatcataa ctaaattttt tgtagataga ggttttcttg agattaaatc accaattctg    720

atacctattg aatatattga aaggatgggg attacagaag ataaagaatt gttcgaacag    780

gtattcaagg ttgataaaaa catgtgcctt agacctatgc tggcacctgg actgtacaat    840

cacctgagaa agtttgataa agttctaccg gatcctataa ggatatttga aattggcccc    900

tgctatagaa aagaatctga cggaagccag catctggaag agtttacaat gctgaatttc    960

tgccagatgg gatcgatgtg cacacgaaag accctggaaa atttgataga tgaattactg   1020

gaatttatgg atattgaata tgaaatcgta tctgataact gccatgtata tggagctact   1080

attgatgttc ttcacaaaga tatggaactt gcatctgctg ttgttgggcc aattccaaaa   1140

gatgcagact ggggaatcac aaaaccatgg atcggtgctg gatttggtct tgaacgtttg   1200

ctcaaagtca tgcataatta taagaatata agacgtgcga gcagatctga gtcgtattat   1260

aatggcataa ctacaaatct ctaa                                          1284


<210>  23
<211>  1245
<212>  DNA
<213>  Methanohalobium evestigatum

<400>  23
atgtctaaaa aatcactggc atcattaata tcagacctcc aagtatgggt ttcaaggagt     60

ggtttactcc atgaaataaa aaattatgaa gtgtcacaaa gatacattca tatggaaatg    120

gattgtgggg aaaaaatcac agtaagaaat tccagaaata gtcgtacagc aagaatatta    180

agacttaaaa aatacaaaaa accctgtaaa aactgcaagg tttctgatga ggttattaac    240

agatttttac aaaaacatac cgacagaact gatacaaaag taactgcttt ttcatattct    300

gaatcaaaga aaaagaaatc caagcaactg ggacacaaaa aaaagaagca atcaaaagtg    360

caggttaacc ccactactga aagtattcaa tcaaacacat cagtttcaga agataaaaca    420

gacaacaaaa ttgagccaga gacatttact tctgcacaga aagaacgaat taatgaacta    480

cttttgcctg gagaaaaaat accattttct aatgaaccat caaaatttaa ggaaatagaa    540

tctgaattgg tcaacaaaag gcgaaatgat ttcaaacaga tgtatgaaaa cgaccgagaa    600

gaacaaattg caaaacttga gagaactatt tcccaatttt ttgttgataa agggtttata    660

gaaattaaag ccccaataat tattgatatt gattctgtta aaaaaatggg tatcgataca    720

gatcataaat tatcaaaaca aatattttat cttgataata aacactgctt gcgacccatg    780

cttgcaccgg gtctctacca gtggcttaaa aattttgaca aaatcctgcc tgatccgatt    840

aaaatttttg aaatcggacc gtgttatcgg aaagagtcag aaggtagcca gcaccttgaa    900

gaatttacaa tgtttaactt ctgtcaaatg ggttctggtg caaacagaga aaaccttcta    960

aatcatatag atgacctgtt aaaacatcta aacatcgact acaaaataat tgatgataac   1020

tgtcatgttt atggtgagac catagatatc gtccacggtg atttagaact ttcatctgct   1080

gttgtaggtc ctgttccaat cgatatgaac tggggaatcg ataaaacatg gattggtgca   1140

ggattaggac tggaaagatt gctaaaggtt aaacacggat ataaaaatat aaaacgcgcc   1200

agtaaatctc attcatatta taatggtata tcaaccaatc tttaa                   1245


<210>  24
<211>  867
<212>  DNA
<213>  Desulfotomaculum gibsoniae

<400>  24
atgtttttaa caaggaggga cccacccttg agcagctttt ggacaaaggt tcaatatcaa     60

cgcctgaaag aactcaatgc ctccggggag cagctggaaa tgggtttttc cgatgcacta    120

agccgtgacc gcgcttttca ggggattgaa catcaactga tgagccaggg aaaacgccat    180

ttggaacagc tgcgcacggt gaagcatcgt cccgccttgc tcgagcttga agagaaatta    240

gcgaaagcat tgcaccaaca gggatttgtt caggtggtga ccccgacgat tattacgaag    300

tcggccttgg ctaagatgac cataggggag gaccatcctt tgttttccca ggttttttgg    360

ttggatggga agaaatgttt gcggccgatg ctggctccca atctatacac tttgtggaga    420

gagcttgagc gcctgtggga taagccgatc cggattttcg agattggaac ctgttaccgg    480

aaagagtccc agggggcaca gcatctcaat gaatttacca tgctgaatct cacggaactg    540

gggactcccc tggaagagcg gcatcaacgt cttgaagaca tggcccgctg ggtgctggaa    600

gctgcgggaa taagggagtt tgagctggtt acggaatcct cggtagttta cggggatacg    660

gtagatgtga tgaagggcga tctggagctg gcttcggggg ccatggggcc ccacttcctt    720

gatgaaaaat gggagatatt tgatccctgg gtaggtctgg gctttggtct ggaaaggctt    780

ctgatgattc gtgaaggaac acaacatgtt cagagtatgg ccagaagcct gagctatctt    840

gatggagtac gcttaaatat caattga                                        867


<210>  25
<211>  1215
<212>  DNA
<213>  Methanohalophilus mahii

<400>  25
atggaaagga aaccactaga tttacttata gacaccaacg gagtgtggct ctctaggaac     60

gggttacttc atggtgtaaa gaacttcgag gtgtcaagaa accatattca tatcactact    120

gactgccaaa gccgctttac agtacgcaat tcaagaagaa gtcgctctgc aagggcgcta    180

cgcaacaata aatatcgcaa agcatgcaaa aactgcaaac tttccgatga gcgtattact    240

cgttttgtca caaaagattt tggcagggga agccaggcac gtgttatcac atcttcaaaa    300

acaaaaaaga gtaaatctcc aaaggaagca gtggtaaaat ctgtatccag caaggcaaat    360

gaaatgccac ctgttgtaga ggcaaaaaaa gaaaagcctg taaaaccgga ttacacgcct    420

gcccagaaga aaaggattac aacactgctt agccctgcag acgaccttag ttcaataaaa    480

gaactcccca ccttcaagga gctggagaca gaacttgtta aaaaaagaaa acaagacctg    540

cgccagatgt atgaggatga ccgcagacat cagctggccc agctcgaaag ggacatctcc    600

ctatttttaa tagaaaaagg attcatggaa gtacgtactt ctgtcctgat acctgccaaa    660

ttcattgaaa gaatgggcat cacagaagaa gaccccctct acaagcagat cttccgggtg    720

gatgagaata catgcctgcg gcccatgctt gccccgggat tatataatta tcttcacaat    780

tttgataaca taatgcccga ccccctcaag atattcgaga tcggtacctg ctacagaaag    840

gaatccgacg gcaaagaaca tcttgaagag tttacaatgg ttaatttctg ccagatgggt    900

tcgggatgca caaaagaaaa cctgttaaat attatcgatg acctgctcaa atatctaaac    960

atcgattacg aagtaatctc ggataattgc atggtgtatg gagataccat tgacataatg   1020

catggggata tggaaatatc ttcagccgtt gtgggaccca ttccacagga cctcgactgg   1080

ggagtgacca aaccctggat gggtgcagga atgggaattg agagattact caaggtaaag   1140

cacaaataca caaacataaa gcgctcaagc aggtctattt catactataa cggaattaca   1200

accaatctca ggtga                                                    1215


<210>  26
<211>  67
<212>  RNA
<213>  Methanosarcina barkeri

<400>  26
ggaaaccuga ucauguagau cguggacucu aaauccgcag ccggguagau ucccgggguu     60

uccgcca                                                               67


<210>  27
<211>  72
<212>  DNA
<213>  Methanosarcina acetivorans

<400>  27
ggaaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt tagattcccg     60

gggtttccgc ca                                                         72


<210>  28
<211>  72
<212>  DNA
<213>  Methanosarcina mazei

<400>  28
ggaaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt tagattcccg     60

gggtttccgc ca                                                         72


<210>  29
<211>  72
<212>  DNA
<213>  Methanococcoides burtonii

<400>  29
ggagacttga tcatgtagat cgaacggact ctaaatcctt tcagccgggt tagattcccg     60

gagtttccgc ca                                                         72


<210>  30
<211>  67
<212>  RNA
<213>  Desulfobacterium hafniense

<400>  30
ggaaaccuga ucauguagau cguggacucu aaauccgcag ccggguagau ucccgggguu     60

uccgcca                                                               67


<210>  31
<211>  101
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: H1/TO promoter

<400>  31
aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt     60

gatagagact tataagttcc ctatcagtga tagagacacc a                        101


<210>  32
<211>  250
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: U6 snRNA promoter

<400>  32
agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga     60

gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag    120

aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca    180

tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg    240

acgaaacacc                                                           250


<210>  33
<211>  269
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: SNR52 promoter

<400>  33
tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt     60

ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt    120

agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt    180

caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga    240

aacttctccg cagtgaaaga taaatgatc                                      269


<210>  34
<211>  221
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: H1 promoter

<400>  34
gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa     60

cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg agtggcgccc    120

tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg    180

gatttgggaa tcttataagt tctgtatgag accacagatc t                        221


<210>  35
<211>  372
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: U6-tRNApyl construct

<400>  35
aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac     60

aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa    120

aatacgtgac gtagaaagta ataatttctt gggtagtttg cagtttttaa aattatgttt    180

taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata    240

tatcttgtgg aaaggacgaa acaccgaatt ctctagactc gagggaaacc tgatcatgta    300

gatcgaatgg actctaaatc cgttcagccg ggttagattc ccggggtttc cggacaagtg    360

cggtttttgt tt                                                        372


<210>  36
<211>  1104
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: GFP nucleotide sequence

<400>  36
atggcctcca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat     60

ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac    120

ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca    180

cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa    240

cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct    300

ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt    360

gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac    420

aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat    480

ggaatcaaag ctaacttcaa aattcgtcac aacattgaag atggatccgt tcaactagca    540

gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat    600

tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc    660

cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatcaggc caagcctttg    720

tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc    780

tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt    840

gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct    900

gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc    960

atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa   1020

gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc   1080

tctggttatg tgtgggaggg ctaa                                          1104


<210>  37
<211>  367
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: GFP amino acid sequence

<400>  37

Met Ala Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Gln Ala Lys Pro Leu 
225                 230                 235                 240 


Ser Gln Glu Glu Ser Thr Leu Ile Glu Arg Ala Thr Ala Thr Ile Asn 
                245                 250                 255     


Ser Ile Pro Ile Ser Glu Asp Tyr Ser Val Ala Ser Ala Ala Leu Ser 
            260                 265                 270         


Ser Asp Gly Arg Ile Phe Thr Gly Val Asn Val Tyr His Phe Thr Gly 
        275                 280                 285             


Gly Pro Cys Ala Glu Leu Val Val Leu Gly Thr Ala Ala Ala Ala Ala 
    290                 295                 300                 


Ala Gly Asn Leu Thr Cys Ile Val Ala Ile Gly Asn Glu Asn Arg Gly 
305                 310                 315                 320 


Ile Leu Ser Pro Cys Gly Arg Cys Arg Gln Val Leu Leu Asp Leu His 
                325                 330                 335     


Pro Gly Ile Lys Ala Ile Val Lys Asp Ser Asp Gly Gln Pro Thr Ala 
            340                 345                 350         


Val Gly Ile Arg Glu Leu Leu Pro Ser Gly Tyr Val Trp Glu Gly 
        355                 360                 365         


<210>  38
<211>  367
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: GFPY40 Amino acid Sequence

<220>
<221>  SITE
<222>  (40)..(40)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  38

Met Ala Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Xaa Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Gln Ala Lys Pro Leu 
225                 230                 235                 240 


Ser Gln Glu Glu Ser Thr Leu Ile Glu Arg Ala Thr Ala Thr Ile Asn 
                245                 250                 255     


Ser Ile Pro Ile Ser Glu Asp Tyr Ser Val Ala Ser Ala Ala Leu Ser 
            260                 265                 270         


Ser Asp Gly Arg Ile Phe Thr Gly Val Asn Val Tyr His Phe Thr Gly 
        275                 280                 285             


Gly Pro Cys Ala Glu Leu Val Val Leu Gly Thr Ala Ala Ala Ala Ala 
    290                 295                 300                 


Ala Gly Asn Leu Thr Cys Ile Val Ala Ile Gly Asn Glu Asn Arg Gly 
305                 310                 315                 320 


Ile Leu Ser Pro Cys Gly Arg Cys Arg Gln Val Leu Leu Asp Leu His 
                325                 330                 335     


Pro Gly Ile Lys Ala Ile Val Lys Asp Ser Asp Gly Gln Pro Thr Ala 
            340                 345                 350         


Val Gly Ile Arg Glu Leu Leu Pro Ser Gly Tyr Val Trp Glu Gly 
        355                 360                 365         


<210>  39
<211>  1413
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-IL-6 (28D2)  Gamma Nucleotide Sequence

<400>  39
atggagactg ggctgcgctg gcttctcctg gtcgctgtgc tcaaaggtgt ccagtgtcag     60

tcgctggggg agtccggggg tcgcctggtc acgcctggga cacccctgac actcacctgc    120

acagtctctg gattatccct cagtaagaat gcaattgcct gggtccgcca ggctccaggg    180

aagggactgg aatggatcgg aatcatttat gctggtggtg ccacaaccta cgcgagctgg    240

gcgaaaggcc gattcaccat ctccaagtcc tcgaccacgg tggatctgaa gatcaccagt    300

ccgacaacag tggacacggc cacctatttc tgtgccaggg aatatgctgg tgatagttat    360

tatactggat acactcagtt ggatctctgg ggcccaggca ccctggtcac cgtctcgagt    420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg    480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc    720

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga    780

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct    840

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg    900

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac    960

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag   1020

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc   1080

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag   1140

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc   1200

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg   1260

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg   1320

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg   1380

cagaagagcc tctccctgtc tccgggtaaa tga                                1413


<210>  40
<211>  470
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-IL-6 (28D2)  Gamma Amino acid Sequence

<400>  40

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Leu Gly Glu Ser Gly Gly Arg Leu Val Thr Pro 
            20                  25                  30          


Gly Thr Pro Leu Thr Leu Thr Cys Thr Val Ser Gly Leu Ser Leu Ser 
        35                  40                  45              


Lys Asn Ala Ile Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Ile Gly Ile Ile Tyr Ala Gly Gly Ala Thr Thr Tyr Ala Ser Trp 
65                  70                  75                  80  


Ala Lys Gly Arg Phe Thr Ile Ser Lys Ser Ser Thr Thr Val Asp Leu 
                85                  90                  95      


Lys Ile Thr Ser Pro Thr Thr Val Asp Thr Ala Thr Tyr Phe Cys Ala 
            100                 105                 110         


Arg Glu Tyr Ala Gly Asp Ser Tyr Tyr Thr Gly Tyr Thr Gln Leu Asp 
        115                 120                 125             


Leu Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  41
<211>  1413
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-IL-6 (28D2)  Gamma_amber K274 Nucleotide
       Sequence

<400>  41
atggagactg ggctgcgctg gcttctcctg gtcgctgtgc tcaaaggtgt ccagtgtcag     60

tcgctggggg agtccggggg tcgcctggtc acgcctggga cacccctgac actcacctgc    120

acagtctctg gattatccct cagtaagaat gcaattgcct gggtccgcca ggctccaggg    180

aagggactgg aatggatcgg aatcatttat gctggtggtg ccacaaccta cgcgagctgg    240

gcgaaaggcc gattcaccat ctccaagtcc tcgaccacgg tggatctgaa gatcaccagt    300

ccgacaacag tggacacggc cacctatttc tgtgccaggg aatatgctgg tgatagttat    360

tatactggat acactcagtt ggatctctgg ggcccaggca ccctggtcac cgtctcgagt    420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg    480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc    720

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga    780

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct    840

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcta gttcaactgg    900

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac    960

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag   1020

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc   1080

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag   1140

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc   1200

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg   1260

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg   1320

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg   1380

cagaagagcc tctccctgtc tccgggtaaa tga                                1413


<210>  42
<211>  470
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-IL-6 (28D2)  Gamma_amber  K274 amino 
       acid Sequence

<220>
<221>  SITE
<222>  (297)..(297)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  42

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Leu Gly Glu Ser Gly Gly Arg Leu Val Thr Pro 
            20                  25                  30          


Gly Thr Pro Leu Thr Leu Thr Cys Thr Val Ser Gly Leu Ser Leu Ser 
        35                  40                  45              


Lys Asn Ala Ile Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Ile Gly Ile Ile Tyr Ala Gly Gly Ala Thr Thr Tyr Ala Ser Trp 
65                  70                  75                  80  


Ala Lys Gly Arg Phe Thr Ile Ser Lys Ser Ser Thr Thr Val Asp Leu 
                85                  90                  95      


Lys Ile Thr Ser Pro Thr Thr Val Asp Thr Ala Thr Tyr Phe Cys Ala 
            100                 105                 110         


Arg Glu Tyr Ala Gly Asp Ser Tyr Tyr Thr Gly Tyr Thr Gln Leu Asp 
        115                 120                 125             


Leu Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Xaa Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  43
<211>  726
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-IL-6 (28D2)  Kappa Nucleotide Sequence

<400>  43
atggacacga gggcccccac tcagctgctg gggctcctgc tgctctggct cccaggcgcc     60

agatgtgccc ttgtgatgac ccagactcca gcctccgtgt ctgccgctgt gggaggcaca    120

gtgaccatca attgccaggc cagtgaggac ctttttagta gtttggcctg gtttcagcag    180

aaaccagggc agcctcccaa actcctgatc tattctgcat ccactctggc atctggggtc    240

ccatcgcggt tcagcggcag tggatctggg acagaattca ctctcaccat cagcgacctg    300

gaatgtgccg atgctgccac ttactactgt ctaggccttt actattatct tactcctgat    360

cctatttatg ggttcggcgg agggaccaag gtggtggtgg tccgtacggt ggctgcacca    420

tctgtcttca tcttcccgcc atctgatgag cagttgaaat ctggaactgc ctctgttgtg    480

tgcctgctga ataacttcta tcccagagag gccaaagtac agtggaaggt ggataacgcc    540

ctccaatcgg gtaactccca ggagagtgtc acagagcagg acagcaagga cagcacctac    600

agcctcagca gcaccctgac gctgagcaaa gcagactacg agaaacacaa agtctacgcc    660

tgcgaagtca cccatcaggg cctgagttcg cccgtcacaa agagcttcaa caggggagag    720

tgttaa                                                               726


<210>  44
<211>  241
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-IL-6 (28D2)  Kappa amino acid Sequence

<400>  44

Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Arg Cys Ala Leu Val Met Thr Gln Thr Pro Ala Ser 
            20                  25                  30          


Val Ser Ala Ala Val Gly Gly Thr Val Thr Ile Asn Cys Gln Ala Ser 
        35                  40                  45              


Glu Asp Leu Phe Ser Ser Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Ser Ala Ser Thr Leu Ala Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Asp Leu Glu Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Leu Gly 
            100                 105                 110         


Leu Tyr Tyr Tyr Leu Thr Pro Asp Pro Ile Tyr Gly Phe Gly Gly Gly 
        115                 120                 125             


Thr Lys Val Val Val Val Arg Thr Val Ala Ala Pro Ser Val Phe Ile 
    130                 135                 140                 


Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val 
145                 150                 155                 160 


Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 
                165                 170                 175     


Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 
            180                 185                 190         


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu 
        195                 200                 205             


Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr 
    210                 215                 220                 


His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu 
225                 230                 235                 240 


Cys 
    


<210>  45
<211>  1413
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5) gamma Nucleotide sequence

<400>  45
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gaggtgcagc tggtggagtc tggcggtggc ttggtacagc cgggcgggtc cctgcgcctc    120

tcctgtgccg cttccggatt caacatcaaa gacacgtata ttcactgggt ccgtcaggca    180

cctggcaagg gtctggagtg ggtgagccgc atttatccta ccaatggtta cactcgctac    240

gccgactctg tgaagggccg cttcaccatc agcgccgaca cgtccaagaa caccctgtat    300

ctgcaaatga acagcctgcg tgccgaggac accgcggtgt attactgcag ccgctggggc    360

ggtgatggct tttacgcgat ggactactgg ggccagggca ccctggtcac cgtctcgagt    420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg    480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc    720

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga    780

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct    840

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg    900

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac    960

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag   1020

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc   1080

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag   1140

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc   1200

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg   1260

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg   1320

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg   1380

cagaagagcc tctccctgtc tccgggtaaa tga                                1413


<210>  46
<211>  470
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5) gamma amino acid sequence

<400>  46

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  47
<211>  1413
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5) gamma_K274amber nucleotide 
       sequence

<400>  47
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gaggtgcagc tggtggagtc tggcggtggc ttggtacagc cgggcgggtc cctgcgcctc    120

tcctgtgccg cttccggatt caacatcaaa gacacgtata ttcactgggt ccgtcaggca    180

cctggcaagg gtctggagtg ggtgagccgc atttatccta ccaatggtta cactcgctac    240

gccgactctg tgaagggccg cttcaccatc agcgccgaca cgtccaagaa caccctgtat    300

ctgcaaatga acagcctgcg tgccgaggac accgcggtgt attactgcag ccgctggggc    360

ggtgatggct tttacgcgat ggactactgg ggccagggca ccctggtcac cgtctcgagt    420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg    480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc    720

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga    780

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct    840

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcta gttcaactgg    900

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac    960

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag   1020

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc   1080

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag   1140

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc   1200

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg   1260

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg   1320

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg   1380

cagaagagcc tctccctgtc tccgggtaaa tga                                1413


<210>  48
<211>  470
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5) gamma_K274amber amino acid 
       sequence

<220>
<221>  SITE
<222>  (297)..(297)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  48

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Xaa Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  49
<211>  1413
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5) gamma_T359amber nucleotide 
       sequence

<400>  49
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gaggtgcagc tggtggagtc tggcggtggc ttggtacagc cgggcgggtc cctgcgcctc    120

tcctgtgccg cttccggatt caacatcaaa gacacgtata ttcactgggt ccgtcaggca    180

cctggcaagg gtctggagtg ggtgagccgc atttatccta ccaatggtta cactcgctac    240

gccgactctg tgaagggccg cttcaccatc agcgccgaca cgtccaagaa caccctgtat    300

ctgcaaatga acagcctgcg tgccgaggac accgcggtgt attactgcag ccgctggggc    360

ggtgatggct tttacgcgat ggactactgg ggccagggca ccctggtcac cgtctcgagt    420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg    480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc    720

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga    780

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct    840

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg    900

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac    960

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag   1020

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc   1080

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag   1140

atgtagaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc   1200

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg   1260

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg   1320

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg   1380

cagaagagcc tctccctgtc tccgggtaaa tga                                1413


<210>  50
<211>  470
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5) gamma_T359amber amino acid  
       sequence

<220>
<221>  SITE
<222>  (382)..(382)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  50

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Xaa Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  51
<211>  705
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5)Kappa nucleotide sequence

<400>  51
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtgggaga ccgtgtcaca    120

atcacttgcc gtgctagcca ggatgtgaat acagcggtgg cctggtatca gcagaaacct    180

ggcaaagccc ctaagctcct gatctattct gcatcctttt tgtacagcgg cgtgccgagc    240

cgcttcagcg gcagccgttc tggtaccgat ttcactctca ccatcagctc tctgcaaccg    300

gaagattttg caacttacta ctgtcaacag cactacacca ctcctccgac gttcggccaa    360

gggaccaagg tggaaatcga acgtacggtg gctgcaccat ctgtcttcat cttcccgcca    420

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat    480

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag    540

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg    600

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc    660

ctgagttcgc ccgtcacaaa gagcttcaac aggggagagt gttaa                    705


<210>  52
<211>  234
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 (4D5)Kappa amino acid sequence

<400>  52

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
        35                  40                  45              


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
            100                 105                 110         


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Glu Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 


<210>  53
<211>  705
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 Kappa D70amber nucleotide sequence

<400>  53
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtgggaga ccgtgtcaca    120

atcacttgcc gtgctagcca ggatgtgaat acagcggtgg cctggtatca gcagaaacct    180

ggcaaagccc ctaagctcct gatctattct gcatcctttt tgtacagcgg cgtgccgagc    240

cgcttcagcg gcagccgttc tggtacctag ttcactctca ccatcagctc tctgcaaccg    300

gaagattttg caacttacta ctgtcaacag cactacacca ctcctccgac gttcggccaa    360

gggaccaagg tggaaatcga acgtacggtg gctgcaccat ctgtcttcat cttcccgcca    420

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat    480

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag    540

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg    600

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc    660

ctgagttcgc ccgtcacaaa gagcttcaac aggggagagt gttaa                    705


<210>  54
<211>  234
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 Kappa D70amber amino acid sequence

<220>
<221>  SITE
<222>  (90)..(90)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  54

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
        35                  40                  45              


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Arg Ser Gly Thr Xaa Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
            100                 105                 110         


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Glu Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 


<210>  55
<211>  705
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 Kappa E81amber nucleotide sequence

<400>  55
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtgggaga ccgtgtcaca    120

atcacttgcc gtgctagcca ggatgtgaat acagcggtgg cctggtatca gcagaaacct    180

ggcaaagccc ctaagctcct gatctattct gcatcctttt tgtacagcgg cgtgccgagc    240

cgcttcagcg gcagccgttc tggtaccgat ttcactctca ccatcagctc tctgcaaccg    300

taggattttg caacttacta ctgtcaacag cactacacca ctcctccgac gttcggccaa    360

gggaccaagg tggaaatcga acgtacggtg gctgcaccat ctgtcttcat cttcccgcca    420

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat    480

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag    540

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg    600

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc    660

ctgagttcgc ccgtcacaaa gagcttcaac aggggagagt gttaa                    705


<210>  56
<211>  234
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-Her2 Kappa E81amber amino acid sequence

<220>
<221>  SITE
<222>  (101)..(101)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  56

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
        35                  40                  45              


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Xaa Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
            100                 105                 110         


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Glu Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 


<210>  57
<211>  825
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-PSMA scFv nucleotide sequence

<400>  57
atggaggcgc cagcgcagct tctcttcttg ctcctgctct ggctgccgga cacgacggga     60

gacatcgtca tgactcagtc acacaagttc atgtcgactt cggtgggaga tagggtgtcc    120

atcatttgca aagcaagcca agatgtaggg acagcggtcg actggtatca gcagaagccc    180

ggtcagtccc ctaaactcct catctactgg gcatcgacgc gacacacggg cgtcccggac    240

cgcttcacgg gatcgggatc aggtactgac tttacattga caattacaaa cgtccaatcg    300

gaggaccttg cggattactt ctgtcaacag tacaattcgt atcccctgac gttcggggct    360

gggacaaagc tcgacttgaa gggcggtgga gggtcaggtg gaggaggctc cggtggggga    420

gggagcggag ggggtggttc ggaggtgcag ttgcagcaat caggcccgga acttaagaaa    480

cccgggacct cagtaagaat cagctgtaag acaagcgggt acacgtttac cgaatatact    540

atccattggg tgaagcagtc gcatggaaaa tcgcttgaat ggatcgggaa cattaatcct    600

aataacgggg gaaccacgta caaccagaag tttgaggata aagccaccct tactgtggac    660

aaatcctcca gcactgccta tatggaattg cggtccctga cctcggagga ttcagccgta    720

tactactgcg cggcaggatg gaattttgat tattgggggc agggaacaac attgacagtc    780

tcgagcggtc cacctcctcc acctcaccat caccatcatc actga                    825


<210>  58
<211>  274
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-PSMA scFv amino acid sequence

<400>  58

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser 
            20                  25                  30          


Thr Ser Val Gly Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln Asp 
        35                  40                  45              


Val Gly Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp 
65                  70                  75                  80  


Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr 
                85                  90                  95      


Asn Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn 
            100                 105                 110         


Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Asp Leu Lys Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Lys Lys 
145                 150                 155                 160 


Pro Gly Thr Ser Val Arg Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Glu Tyr Thr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu 
            180                 185                 190         


Glu Trp Ile Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn 
        195                 200                 205             


Gln Lys Phe Glu Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
    210                 215                 220                 


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr 
                245                 250                 255     


Thr Leu Thr Val Ser Ser Gly Pro Pro Pro Pro Pro His His His His 
            260                 265                 270         


His His 
        


<210>  59
<211>  828
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-PSMA scFv_117amber nucleotide sequence

<400>  59
atggaggcgc cagcgcagct tctcttcttg ctcctgctct ggctgccgga cacgacggga     60

gacatcgtca tgactcagtc acacaagttc atgtcgactt cggtgggaga tagggtgtcc    120

atcatttgca aagcaagcca agatgtaggg acagcggtcg actggtatca gcagaagccc    180

ggtcagtccc ctaaactcct catctactgg gcatcgacgc gacacacggg cgtcccggac    240

cgcttcacgg gatcgggatc aggtactgac tttacattga caattacaaa cgtccaatcg    300

gaggaccttg cggattactt ctgtcaacag tacaattcgt atcccctgac gttcggggct    360

gggacaaagc tcgacttgaa gggcggtgga gggtcaggtg gaggaggctc cggtggggga    420

gggagcggag ggggtggttc ggaggtgcag ttgcagcaat caggcccgga acttaagaaa    480

cccgggacct cagtaagaat cagctgtaag acaagcgggt acacgtttac cgaatatact    540

atccattggg tgaagcagtc gcatggaaaa tcgcttgaat ggatcgggaa cattaatcct    600

aataacgggg gaaccacgta caaccagaag tttgaggata aagccaccct tactgtggac    660

aaatcctcca gcactgccta tatggaattg cggtccctga cctcggagga ttcagccgta    720

tactactgcg cggcaggatg gaattttgat tattgggggc agggaacaac attgacagtc    780

tcgagctagg gtccacctcc tccacctcac catcaccatc atcactga                 828


<210>  60
<211>  275
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: anti-PSMA scFv_amber amino acid sequence

<220>
<221>  SITE
<222>  (263)..(263)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  60

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser 
            20                  25                  30          


Thr Ser Val Gly Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln Asp 
        35                  40                  45              


Val Gly Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp 
65                  70                  75                  80  


Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr 
                85                  90                  95      


Asn Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn 
            100                 105                 110         


Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Asp Leu Lys Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Lys Lys 
145                 150                 155                 160 


Pro Gly Thr Ser Val Arg Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Glu Tyr Thr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu 
            180                 185                 190         


Glu Trp Ile Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn 
        195                 200                 205             


Gln Lys Phe Glu Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
    210                 215                 220                 


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr 
                245                 250                 255     


Thr Leu Thr Val Ser Ser Xaa Gly Pro Pro Pro Pro Pro His His His 
            260                 265                 270         


His His His 
        275 


<210>  61
<211>  612
<212>  DNA
<213>  Homo sapiens

<400>  61
atgtctgcac ttctgatcct agctcttgtt ggagctgcag ttgctaagct tcaccatcac     60

catcaccatc cgattccaga ctcatctccg ttgctgcagt ttggcgggca ggtgcggcag    120

cgctatctgt acaccgacga cgcacagcaa acagaggctc atcttgaaat ccgggaggat    180

ggcactgttg gcggtgcggc ggatcagagt cccgagtcac tgcttcaact taaggccttg    240

aaaccaggag tgattcaaat cctcggtgtg aaaacgagta gattcctctg ccaaaggccc    300

gatggcgccc tgtacggaag cctccacttc gaccctgagg catgtagctt tcgcgaactc    360

ctgttggaag atgggtataa cgtctatcag tccgaggcac acggccttcc tctccacctc    420

cccgggaata agtcaccgca cagggacccc gctccaaggg gtcccgcacg attcctgccc    480

ttgccagggc tgccacccgc cctgccagaa ccgcctggaa ttctggcccc tcagccacct    540

gacgtcgggt ctagcgaccc cctgagtatg gtaggaccta gccagggcag atccccctcc    600

tacgcctcct aa                                                        612


<210>  62
<211>  219
<212>  PRT
<213>  Homo sapiens

<400>  62

Met Ser Ala Leu Leu Ile Leu Ala Leu Val Gly Ala Ala Val Ala Asp 
1               5                   10                  15      


Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr Lys 
            20                  25                  30          


Asp Asp Asp Asp Lys Ser His Pro Ile Pro Asp Ser Ser Pro Leu Leu 
        35                  40                  45              


Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala 
    50                  55                  60                  


Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly 
65                  70                  75                  80  


Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu 
                85                  90                  95      


Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu 
            100                 105                 110         


Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro 
        115                 120                 125             


Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val 
    130                 135                 140                 


Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His Leu Pro Gly Asn Lys 
145                 150                 155                 160 


Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro 
                165                 170                 175     


Leu Pro Gly Leu Pro Pro Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala 
            180                 185                 190         


Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly 
        195                 200                 205             


Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
    210                 215                 


<210>  63
<211>  612
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 R131amber nucleotide sequence

<400>  63
atgtctgcac ttctgatcct agctcttgtt ggagctgcag ttgctaagct tcaccatcac     60

catcaccatc cgattccaga ctcatctccg ttgctgcagt ttggcgggca ggtgcggcag    120

cgctatctgt acaccgacga cgcacagcaa acagaggctc atcttgaaat ccgggaggat    180

ggcactgttg gcggtgcggc ggatcagagt cccgagtcac tgcttcaact taaggccttg    240

aaaccaggag tgattcaaat cctcggtgtg aaaacgagta gattcctctg ccaaaggccc    300

gatggcgccc tgtacggaag cctccacttc gaccctgagg catgtagctt tcgcgaactc    360

ctgttggaag atgggtataa cgtctatcag tccgaggcac acggccttcc tctccacctc    420

cccgggaata agtcaccgca cagggacccc gctccatagg gtcccgcacg attcctgccc    480

ttgccagggc tgccacccgc cctgccagaa ccgcctggaa ttctggcccc tcagccacct    540

gacgtcgggt ctagcgaccc cctgagtatg gtaggaccta gccagggcag atccccctcc    600

tacgcctcct aa                                                        612


<210>  64
<211>  219
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 R131amber amino acid sequence

<220>
<221>  SITE
<222>  (169)..(169)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  64

Met Ser Ala Leu Leu Ile Leu Ala Leu Val Gly Ala Ala Val Ala Asp 
1               5                   10                  15      


Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr Lys 
            20                  25                  30          


Asp Asp Asp Asp Lys Ser His Pro Ile Pro Asp Ser Ser Pro Leu Leu 
        35                  40                  45              


Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala 
    50                  55                  60                  


Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly 
65                  70                  75                  80  


Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu 
                85                  90                  95      


Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu 
            100                 105                 110         


Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro 
        115                 120                 125             


Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val 
    130                 135                 140                 


Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His Leu Pro Gly Asn Lys 
145                 150                 155                 160 


Ser Pro His Arg Asp Pro Ala Pro Xaa Gly Pro Ala Arg Phe Leu Pro 
                165                 170                 175     


Leu Pro Gly Leu Pro Pro Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala 
            180                 185                 190         


Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly 
        195                 200                 205             


Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
    210                 215                 


<210>  65
<211>  660
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 F12amber nucleotide sequence

<400>  65
atgtctgctc ttctgatact cgccttggta ggggctgctg ttgccgacta taaggatcac     60

gacggcgatt acaaggacca tgatatcgat tacaaagatg acgacgacaa gtctcatccg    120

attccagact catctccgtt gctgcagtag ggcgggcagg tgcggcagcg ctatctgtac    180

accgacgacg cacagcaaac agaggctcat cttgaaatcc gggaggatgg cactgttggc    240

ggtgcggcgg atcagagtcc cgagtcactg cttcaactta aggccttgaa accaggagtg    300

attcaaatcc tcggtgtgaa aacgagtaga ttcctctgcc aaaggcccga tggcgccctg    360

tacggaagcc tccacttcga ccctgaggca tgtagctttc gcgaactcct gttggaagat    420

gggtataacg tctatcagtc cgaggcacac ggccttcctc tccacctccc cgggaataag    480

tcaccgcaca gggaccccgc tccaaggggt cccgcacgat tcctgccctt gccagggctg    540

ccacccgccc tgccagaacc gcctggaatt ctggcccctc agccacctga cgtcgggtct    600

agcgaccccc tgagtatggt aggacctagc cagggcagat ccccctccta cgcctcctaa    660


<210>  66
<211>  219
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 F12amber amino acid sequence

<220>
<221>  SITE
<222>  (50)..(50)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  66

Met Ser Ala Leu Leu Ile Leu Ala Leu Val Gly Ala Ala Val Ala Asp 
1               5                   10                  15      


Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr Lys 
            20                  25                  30          


Asp Asp Asp Asp Lys Ser His Pro Ile Pro Asp Ser Ser Pro Leu Leu 
        35                  40                  45              


Gln Xaa Gly Gly Gln Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala 
    50                  55                  60                  


Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly 
65                  70                  75                  80  


Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu 
                85                  90                  95      


Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu 
            100                 105                 110         


Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro 
        115                 120                 125             


Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val 
    130                 135                 140                 


Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His Leu Pro Gly Asn Lys 
145                 150                 155                 160 


Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro 
                165                 170                 175     


Leu Pro Gly Leu Pro Pro Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala 
            180                 185                 190         


Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly 
        195                 200                 205             


Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
    210                 215                 


<210>  67
<211>  612
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 L60amber nucleotide sequence

<400>  67
atgtctgcac ttctgatcct agctcttgtt ggagctgcag ttgctaagct tcaccatcac     60

catcaccatc cgattccaga ctcatctccg ttgctgcagt ttggcgggca ggtgcggcag    120

cgctatctgt acaccgacga cgcacagcaa acagaggctc atcttgaaat ccgggaggat    180

ggcactgttg gcggtgcggc ggatcagagt cccgagtcac tgcttcaact taaggccttg    240

aaaccaggag tgattcaaat ctagggtgtg aaaacgagta gattcctctg ccaaaggccc    300

gatggcgccc tgtacggaag cctccacttc gaccctgagg catgtagctt tcgcgaactc    360

ctgttggaag atgggtataa cgtctatcag tccgaggcac acggccttcc tctccacctc    420

cccgggaata agtcaccgca cagggacccc gctccaaggg gtcccgcacg attcctgccc    480

ttgccagggc tgccacccgc cctgccagaa ccgcctggaa ttctggcccc tcagccacct    540

gacgtcgggt ctagcgaccc cctgagtatg gtaggaccta gccagggcag atccccctcc    600

tacgcctcct aa                                                        612


<210>  68
<211>  219
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 L60amber amino acid sequence

<220>
<221>  SITE
<222>  (104)..(104)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  68

Met Ser Ala Leu Leu Ile Leu Ala Leu Val Gly Ala Ala Val Ala Asp 
1               5                   10                  15      


Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr Lys 
            20                  25                  30          


Asp Asp Asp Asp Lys Ser His Pro Ile Pro Asp Ser Ser Pro Leu Leu 
        35                  40                  45              


Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala 
    50                  55                  60                  


Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly 
65                  70                  75                  80  


Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu 
                85                  90                  95      


Lys Pro Gly Val Ile Gln Ile Xaa Gly Val Lys Thr Ser Arg Phe Leu 
            100                 105                 110         


Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro 
        115                 120                 125             


Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val 
    130                 135                 140                 


Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His Leu Pro Gly Asn Lys 
145                 150                 155                 160 


Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro 
                165                 170                 175     


Leu Pro Gly Leu Pro Pro Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala 
            180                 185                 190         


Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly 
        195                 200                 205             


Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
    210                 215                 


<210>  69
<211>  612
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21P 90amber nucleotide sequence

<400>  69
atgtctgcac ttctgatcct agctcttgtt ggagctgcag ttgctaagct tcaccatcac     60

catcaccatc cgattccaga ctcatctccg ttgctgcagt ttggcgggca ggtgcggcag    120

cgctatctgt acaccgacga cgcacagcaa acagaggctc atcttgaaat ccgggaggat    180

ggcactgttg gcggtgcggc ggatcagagt cccgagtcac tgcttcaact taaggccttg    240

aaaccaggag tgattcaaat cctcggtgtg aaaacgagta gattcctctg ccaaaggccc    300

gatggcgccc tgtacggaag cctccacttc gactaggagg catgtagctt tcgcgaactc    360

ctgttggaag atgggtataa cgtctatcag tccgaggcac acggccttcc tctccacctc    420

cccgggaata agtcaccgca cagggacccc gctccaaggg gtcccgcacg attcctgccc    480

ttgccagggc tgccacccgc cctgccagaa ccgcctggaa ttctggcccc tcagccacct    540

gacgtcgggt ctagcgaccc cctgagtatg gtaggaccta gccagggcag atccccctcc    600

tacgcctcct aa                                                        612


<210>  70
<211>  219
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 P90amber  amino acid sequence

<220>
<221>  SITE
<222>  (128)..(128)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  70

Met Ser Ala Leu Leu Ile Leu Ala Leu Val Gly Ala Ala Val Ala Asp 
1               5                   10                  15      


Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr Lys 
            20                  25                  30          


Asp Asp Asp Asp Lys Ser His Pro Ile Pro Asp Ser Ser Pro Leu Leu 
        35                  40                  45              


Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala 
    50                  55                  60                  


Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly 
65                  70                  75                  80  


Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu 
                85                  90                  95      


Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu 
            100                 105                 110         


Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Xaa 
        115                 120                 125             


Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val 
    130                 135                 140                 


Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His Leu Pro Gly Asn Lys 
145                 150                 155                 160 


Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro 
                165                 170                 175     


Leu Pro Gly Leu Pro Pro Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala 
            180                 185                 190         


Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly 
        195                 200                 205             


Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
    210                 215                 


<210>  71
<211>  612
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 P140amber nucleotide sequence

<400>  71
atgtctgcac ttctgatcct agctcttgtt ggagctgcag ttgctaagct tcaccatcac     60

catcaccatc cgattccaga ctcatctccg ttgctgcagt ttggcgggca ggtgcggcag    120

cgctatctgt acaccgacga cgcacagcaa acagaggctc atcttgaaat ccgggaggat    180

ggcactgttg gcggtgcggc ggatcagagt cccgagtcac tgcttcaact taaggccttg    240

aaaccaggag tgattcaaat cctcggtgtg aaaacgagta gattcctctg ccaaaggccc    300

gatggcgccc tgtacggaag cctccacttc gaccctgagg catgtagctt tcgcgaactc    360

ctgttggaag atgggtataa cgtctatcag tccgaggcac acggccttcc tctccacctc    420

cccgggaata agtcaccgca cagggacccc gctccaaggg gtcccgcacg attcctgccc    480

ttgtaggggc tgccacccgc cctgccagaa ccgcctggaa ttctggcccc tcagccacct    540

gacgtcgggt ctagcgaccc cctgagtatg gtaggaccta gccagggcag atccccctcc    600

tacgcctcct aa                                                        612


<210>  72
<211>  219
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: FGF21 P140amber amino acid sequence

<220>
<221>  SITE
<222>  (178)..(178)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  72

Met Ser Ala Leu Leu Ile Leu Ala Leu Val Gly Ala Ala Val Ala Asp 
1               5                   10                  15      


Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr Lys 
            20                  25                  30          


Asp Asp Asp Asp Lys Ser His Pro Ile Pro Asp Ser Ser Pro Leu Leu 
        35                  40                  45              


Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala 
    50                  55                  60                  


Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly 
65                  70                  75                  80  


Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu 
                85                  90                  95      


Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu 
            100                 105                 110         


Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro 
        115                 120                 125             


Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val 
    130                 135                 140                 


Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His Leu Pro Gly Asn Lys 
145                 150                 155                 160 


Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro 
                165                 170                 175     


Leu Xaa Gly Leu Pro Pro Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala 
            180                 185                 190         


Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly 
        195                 200                 205             


Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
    210                 215                 


<210>  73
<211>  1104
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: GFPY40 Nucleotide Sequence

<400>  73
atggcctcca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat     60

ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatag    120

ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca    180

cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa    240

cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct    300

ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt    360

gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac    420

aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat    480

ggaatcaaag ctaacttcaa aattcgtcac aacattgaag atggatccgt tcaactagca    540

gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat    600

tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc    660

cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatcaggc caagcctttg    720

tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc    780

tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt    840

gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct    900

gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc    960

atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa   1020

gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc   1080

tctggttatg tgtgggaggg ctaa                                          1104


<210>  74
<211>  1416
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Herceptin Nucleotide sequence Gamma Chain

<400>  74
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gaggtgcagc tggtggagtc tggcggtggc ttggtacagc cgggcgggtc cctgcgcctc    120

tcctgtgccg cttccggatt caacatcaaa gacacgtata ttcactgggt ccgtcaggca    180

cctggcaagg gtctggagtg ggtggcccgc atttatccta ccaatggtta cactcgctac    240

gccgactctg tgaagggccg cttcaccatc agcgccgaca cgtccaagaa caccgcttat    300

ctgcaaatga acagcctgcg tgccgaggac accgcggtgt attactgcag ccgctggggc    360

ggtgatggct tttacgcgat ggactactgg ggccagggca ccctggtcac cgtctcgagt    420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg    480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc    720

cctaaatctt gtgacaaaac tcacacatgc ccaccgtgcc cagcacctga actcctgggg    780

ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc    840

cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac    900

tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac    960

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc   1020

aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc   1080

tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggac   1140

gagctcacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta tcccagcgac   1200

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc   1260

gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga caagagcagg   1320

tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac   1380

acgcagaaga gcctctccct gtctccgggt aaataa                             1416


<210>  75
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Herceptin amino acid sequence Gamma Chain

<400>  75

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
                245                 250                 255     


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            260                 265                 270         


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        275                 280                 285             


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    290                 295                 300                 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
305                 310                 315                 320 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                325                 330                 335     


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            340                 345                 350         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        355                 360                 365             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    370                 375                 380                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
385                 390                 395                 400 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                405                 410                 415     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            420                 425                 430         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        435                 440                 445             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    450                 455                 460                 


Leu Ser Leu Ser Pro Gly Lys 
465                 470     


<210>  76
<211>  1416
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Herceptin H274 Nucleotide sequence Gamma 
       Chain

<400>  76
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gaggtgcagc tggtggagtc tggcggtggc ttggtacagc cgggcgggtc cctgcgcctc    120

tcctgtgccg cttccggatt caacatcaaa gacacgtata ttcactgggt ccgtcaggca    180

cctggcaagg gtctggagtg ggtggcccgc atttatccta ccaatggtta cactcgctac    240

gccgactctg tgaagggccg cttcaccatc agcgccgaca cgtccaagaa caccgcttat    300

ctgcaaatga acagcctgcg tgccgaggac accgcggtgt attactgcag ccgctggggc    360

ggtgatggct tttacgcgat ggactactgg ggccagggca ccctggtcac cgtctcgagt    420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg    480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc    720

cctaaatctt gtgacaaaac tcacacatgc ccaccgtgcc cagcacctga actcctgggg    780

ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc    840

cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt ctagttcaac    900

tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac    960

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc   1020

aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc   1080

tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggac   1140

gagctcacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta tcccagcgac   1200

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc   1260

gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga caagagcagg   1320

tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac   1380

acgcagaaga gcctctccct gtctccgggt aaataa                             1416


<210>  77
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Herceptin 274 amino acid sequence Gamma Chain

<220>
<221>  SITE
<222>  (298)..(298)
<223>  Xaa is nnAA, a non natural amino acid.

<400>  77

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
                245                 250                 255     


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            260                 265                 270         


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        275                 280                 285             


Asp Val Ser His Glu Asp Pro Glu Val Xaa Phe Asn Trp Tyr Val Asp 
    290                 295                 300                 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
305                 310                 315                 320 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                325                 330                 335     


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            340                 345                 350         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        355                 360                 365             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    370                 375                 380                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
385                 390                 395                 400 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                405                 410                 415     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            420                 425                 430         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        435                 440                 445             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    450                 455                 460                 


Leu Ser Leu Ser Pro Gly Lys 
465                 470     


<210>  78
<211>  705
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Herceptin nucleotide sequence Kappa Chain

<400>  78
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt     60

gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtgggaga ccgtgtcaca    120

atcacttgcc gtgctagcca ggatgtgaat acagcggtgg cctggtatca gcagaaacct    180

ggcaaagccc ctaagctcct gatctattct gcatcctttt tgtacagcgg cgtgccgagc    240

cgcttcagcg gcagccgttc tggtaccgat ttcactctca ccatcagctc tctgcaaccg    300

gaagattttg caacttacta ctgtcaacag cactacacca ctcctccgac gttcggccaa    360

gggaccaagg tggaaatcaa acgtacggtg gctgcaccat ctgtcttcat cttcccgcca    420

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat    480

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag    540

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg    600

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc    660

ctgagttcgc ccgtcacaaa gagcttcaac aggggagagt gttaa                    705


<210>  79
<211>  234
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Herceptin amino acid sequence Kappa Chain

<400>  79

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
        35                  40                  45              


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
            100                 105                 110         


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 


<210>  80
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: 3 x Flag tag sequence

<400>  80

Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr 
1               5                   10                  15      


Lys Asp Asp Asp Asp Lys Ser 
            20              


<210>  81
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: 5xPro-6xHis tag

<400>  81

Pro Pro Pro Pro Pro His His His His His His 
1               5                   10      


<210>  82
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  1

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  83
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  1

Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
1               5                   10                  15      


Gln 
    


<210>  84
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  2

Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
1               5                   10                  15  
