﻿SEQUENCE LISTING

<110>	Lonza Biologics PLC
<120>	Expression vectors comprising chimeric cytomegalovirus promoter and enhancer sequences

<130>	827-1 PCT

<140>	not assigned yet
<141>	2013-09-23

<150>	EP12185728.8
<151>	2012-09-24


<160>	15

<170>	BiSSAP Version 1.1

<210>	1
<211>	5908
<212>	DNA
<213>	artificial sequence

<220>
<223>	plasmid vector pRY42

<220>
<221>	polyA_signal
<222>	(7)...(245)
<223>	SV40 polyA signal

<220>
<221>	promoter
<222>	(266)...(756)
<223>	mCMV-MIE (IE1) promoter fragment

<220>
<221>	5'UTR
<222>	(758)...(791)
<223>	part of mCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	5'UTR
<222>	(803)...(884)
<223>	part of hCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	intron
<222>	(885)...(1711)
<223>	hCMV-MIE (IE1) intron A

<220>
<221>	5'UTR
<222>	(1712)...(1729)
<223>	hCMV-MIE (IE1) 5'UTR exon 2

<220>
<221>	polyA_signal
<222>	(1767)...(2005)
<223>	SV40 polyA signal

<220>
<221>	promoter
<222>	(2019)...(2345)
<223>	SV40 early promoter, derived from pFRT/lacZeo (Invitrogen)

<220>
<221>	source
<222>	(2350)...(2354)
<223>	sequence containing ATG site found upstream of wild-type FRT in pFRT/lacZeo (Invitrogen)

<220>
<221>	misc_recomb
<222>	(2355)...(2402)
<223>	wild-type flippase recognition target (FRT) site, derived from pcDNA5-Frt (Invitrogen)

<220>
<221>	source
<222>	(3183)...(4043)
<223>	reverse complement CDS of beta-lactamase gene

<220>
<221>	misc_recomb
<222>	(4319)...(4366)
<223>	mutant flippase recognition target (FRT) site

<220>
<221>	promoter
<222>	(4410)...(4900)
<223>	mCMV-MIE (IE1) promoter fragment

<220>
<221>	5'UTR
<222>	(4902)...(4935)
<223>	part of mCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	5'UTR
<222>	(4947)...(5028)
<223>	part of hCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	intron
<222>	(5029)...(5855)
<223>	hCMV-MIE (IE1) intron A

<220>
<221>	5'UTR
<222>	(5856)...(5873)
<223>	hCMV-MIE (IE1)5'UTR exon 2

<400>	1

gaattcattg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa	60
cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt	120
gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa	180
agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca	240
tgtctggcgg ccgctgaggc gcgcctactg agtcattagg gactttccaa tgggttttgc	300
ccagtacata aggtcaatag gggtgaatca acaggaaagt cccattggag ccaagtacac	360
tgagtcaata gggactttcc attgggtttt gcccagtaca aaaggtcaat agggggtgag	420
tcaatgggtt tttcccatta ttggcacgta cataaggtca ataggggtga gtcattgggt	480
ttttccagcc aatttaatta aaacgccatg tactttccca ccattgacgt caatgggcta	540
ttgaaactaa tgcaacgtga cctttaaacg gtactttccc atagctgatt aatgggaaag	600
taccgttctc gagccaatac acgtcaatgg gaagtgaaag ggcagccaaa acgtaacacc	660
gccccggttt tcccctggaa attccatatt ggcacgcatt ctattggctg agctgcgttc	720
tacgtgggta taagaggcgc gaccagcgtc ggtaccgtcg cagtcttcgg tctgaccacc	780
gtagaacgca gcctcaggac ctccatagaa gacaccggga ccgatccagc ctccgcggcc	840
gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata	900
gagtctatag gcccaccccc ttggcttctt atgcatgcta tactgttttt ggcttggggt	960
ctatacaccc ccgcttcctc atgttatagg tgatggtata gcttagccta taggtgtggg	1020
ttattgacca ttattgacca ctcccctatt ggtgacgata ctttccatta ctaatccata	1080
acatggctct ttgccacaac tctctttatt ggctatatgc caatacactg tccttcagag	1140
actgacacgg actctgtatt tttacaggat ggggtctcat ttattattta caaattcaca	1200
tatacaacac caccgtcccc agtgcccgca gtttttatta aacataacgt gggatctcca	1260
cgcgaatctc gggtacgtgt tccggacatg ggctcttctc cggtagcggc ggagcttcta	1320
catccgagcc ctgctcccat gcctccagcg actcatggtc gctcggcagc tccttgctcc	1380
taacagtgga ggccagactt aggcacagca cgatgcccac caccaccagt gtgccgcaca	1440
aggccgtggc ggtagggtat gtgtctgaaa atgagctcgg ggagcgggct tgcaccgctg	1500
acgcatttgg aagacttaag gcagcggcag aagaagatgc aggcagctga gttgttgtgt	1560
tctgataaga gtcagaggta actcccgttg cggtgctgtt aacggtggag ggcagtgtag	1620
tctgagcagt actcgttgct gccgcgcgcg ccaccagaca taatagctga cagactaaca	1680
gactgttcct ttccatgggt cttttctgca gtcaccgtcc ttgacacggg atccggcgcg	1740
cccctagggg taccgtcgac tcgcgaattg atcataatca gccataccac atttgtagag	1800
gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat	1860
gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc	1920
atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa	1980
ctcatcaatg tatcttatca tgtctggatc agcttgagca gctgtggaat gtgtgtcagt	2040
tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca	2100
attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa	2160
gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc	2220
taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg	2280
cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg	2340
gaggctacca tggagaagtt actattccga agttcctatt ctctagaaag tataggaact	2400
tctcgggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa	2460
aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt	2520
ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg	2580
tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc	2640
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc	2700
gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta	2760
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct	2820
acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc	2880
tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa	2940
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa	3000
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa	3060
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt	3120
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac	3180
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc	3240
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc	3300
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata	3360
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc	3420
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc	3480
aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca	3540
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa	3600
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca	3660
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt	3720
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt	3780
tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg	3840
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga	3900
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc	3960
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg	4020
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag	4080
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg	4140
gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg	4200
acattaacct ataaaaatag gcgtatcacg aggccctgat ggctctttgc ggcacccatc	4260
gttcgtaatg ttccgtggca ccgaggacaa ccctcaagag aaaatgtaat cacactggga	4320
agttcctatt ccgaagttcc tattcttcaa aaggtatagg aacttcctgc agtgaataat	4380
aaaatgtgtg tttgtccgaa atacgcgcct actgagtcat tagggacttt ccaatgggtt	4440
ttgcccagta cataaggtca ataggggtga atcaacagga aagtcccatt ggagccaagt	4500
acactgagtc aatagggact ttccattggg ttttgcccag tacaaaaggt caataggggg	4560
tgagtcaatg ggtttttccc attattggca cgtacataag gtcaataggg gtgagtcatt	4620
gggtttttcc agccaattta attaaaacgc catgtacttt cccaccattg acgtcaatgg	4680
gctattgaaa ctaatgcaac gtgaccttta aacggtactt tcccatagct gattaatggg	4740
aaagtaccgt tctcgagcca atacacgtca atgggaagtg aaagggcagc caaaacgtaa	4800
caccgccccg gttttcccct ggaaattcca tattggcacg cattctattg gctgagctgc	4860
gttctacgtg ggtataagag gcgcgaccag cgtcggtacc gtcgcagtct tcggtctgac	4920
caccgtagaa cgcagcctca ggacctccat agaagacacc gggaccgatc cagcctccgc	4980
ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtgacgt aagtaccgcc	5040
tatagagtct ataggcccac ccccttggct tcttatgcat gctatactgt ttttggcttg	5100
gggtctatac acccccgctt cctcatgtta taggtgatgg tatagcttag cctataggtg	5160
tgggttattg accattattg accactcccc tattggtgac gatactttcc attactaatc	5220
cataacatgg ctctttgcca caactctctt tattggctat atgccaatac actgtccttc	5280
agagactgac acggactctg tatttttaca ggatggggtc tcatttatta tttacaaatt	5340
cacatataca acaccaccgt ccccagtgcc cgcagttttt attaaacata acgtgggatc	5400
tccacgcgaa tctcgggtac gtgttccgga catgggctct tctccggtag cggcggagct	5460
tctacatccg agccctgctc ccatgcctcc agcgactcat ggtcgctcgg cagctccttg	5520
ctcctaacag tggaggccag acttaggcac agcacgatgc ccaccaccac cagtgtgccg	5580
cacaaggccg tggcggtagg gtatgtgtct gaaaatgagc tcggggagcg ggcttgcacc	5640
gctgacgcat ttggaagact taaggcagcg gcagaagaag atgcaggcag ctgagttgtt	5700
gtgttctgat aagagtcaga ggtaactccc gttgcggtgc tgttaacggt ggagggcagt	5760
gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag ctgacagact	5820
aacagactgt tcctttccat gggtcttttc tgcagtcacc gtccttgaca cgaagcttac	5880
cggtagatct gctagcacat gtaggcct						5908

<210>	2
<211>	7948
<212>	DNA
<213>	artificial sequence

<220>
<223>	plasmid vector pRY57

<220>
<221>	CDS
<222>	(67)...(713)
<223>	gene optimized cB72.3 kappa light chain

<220>
<221>	polyA_signal
<222>	(720)...(958)
<223>	SV40 polyA signal

<220>
<221>	promoter
<222>	(979)...(1470)
<223>	mCMV-MIE (IE1) promoter fragment

<220>
<221>	5'UTR
<222>	(1471)...(1504)
<223>	part of mCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	5'UTR
<222>	(1516)...(1601)
<223>	part of hCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	intron
<222>	(1598)...(2424)
<223>	hCMV-MIE (IE1) intron A

<220>
<221>	5'UTR
<222>	(2425)...(2441)
<223>	hCMV-MIE (IE1) 5'UTR exon 2

<220>
<221>	CDS
<222>	(2508)...(3836)
<223>	gene optimized cB72.3 gamma-4 heavy chain

<220>
<221>	polyA_signal
<222>	(3843)...(4081)
<223>	SV40 polyA signal

<220>
<221>	promoter
<222>	(4095)...(4421)
<223>	SV40 early promoter, derived from pFRT/lacZeo (Invitrogen)

<220>
<221>	source
<222>	(4426)...(4430)
<223>	sequence containing ATG site found upstream of wild-type FRT in pFRT/lacZeo (Invitrogen)

<220>
<221>	misc_recomb
<222>	(4431)...(4478)
<223>	wild-type flippase recognition target (FRT) site, derived from pcDNA5-Frt (Invitrogen)

<220>
<221>	source
<222>	(5259)...(6119)
<223>	reverse complement CDS of beta-lactamase gene

<220>
<221>	misc_recomb
<222>	(6395)...(6442)
<223>	mutant flippase recognition target (FRT) site

<220>
<221>	promoter
<222>	(6486)...(6977)
<223>	mCMV-MIE (IE1) promoter fragment

<220>
<221>	5'UTR
<222>	(6978)...(7011)
<223>	part of mCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	5'UTR
<222>	(7023)...(7108)
<223>	part of hCMV-MIE (IE1) 5'UTR exon 1

<220>
<221>	intron
<222>	(7105)...(7931)
<223>	hCMV-MIE (IE1) intron A

<220>
<221>	5'UTR
<222>	(7932)...(7948)
<223>	hCMV-MIE (IE1)5'UTR exon 2

<400>	2

aagcttgccg ccaccatgat gcggcctatc gtgctggtgc tgctgttcgc cacctctgcc	60
ctggcc gac atc cag atg acc cag tcc ccc gcc tcc ctg tct gtg tcc		108
       Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Val Ser
       1               5                   10
gtg ggc gag aca gtg acc atc acc tgt cgg gcc tcc gag aac atc tac 	156
Val Gly Asp Thr Val Thr Ile Thr Cys Arg Ala Ser Glu Asn Ile Tyr
15                  20                  25                  30
tcc aac ctg gcc tgg tat cag cag aag cag ggc aag tcc cct cag ctg		204
Ser Asn Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu
                35                  40                  45
ctg gtg tac gcc gcc acc aac ctg gct gac ggc gtg ccc tcc agg ttc		252
Leu Val Tyr Ala Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe
            50                  55                  60
tcc ggc tct ggc tcc ggc acc cag tac tcc ctg aag atc aac tcc ctg		300
Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn Ser Leu
        65                  70                  75 
cag tcc gag gac ttc ggc tcc tac tac tgc cag cac ttc tgg ggc acc		348
Gln Ser Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp Gly Thr
    80                  85                  90
cct tac acc ttc ggc gga ggc acc cgg ctg gaa atc aag cgg acc gtg		396
Pro Tyr Thr Phe Gly Gly Gly Thr Arg Leu Glu Ile Lys Arg Thr Val
95                  100                 105                 110
gcc gct cct tcc gtg ttc atc ttc cca cct tcc gac gag cag ctg aag		444
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys
                115                 120                 125
tcc ggc acc gcc tct gtg gtg tgc ctg ctg aac aac ttc tac cct cgg		492
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg
            130                 135                 140
gag gcc aag gtg cag tgg aag gtg gac aac gcc ctg cag agc ggc aac		540
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn
        145                 150                 155
tcc cag gaa tcc gtc acc gag cag gac tcc aag gac tct acc tac tcc		588
Ser Gln Asp Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser
    160                 165                 170
ctg tcc tcc acc ctg acc ctg tcc aag gcc gac tac gag aag cac aag		636
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys
175                 180                 185                 190
gtg tac gcc tgc gaa gtg acc cac cag ggc ctg tcc agc cct gtg acc		684
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr
                195                 200                 205
aag tcc ttc aac cgg ggc gag tgc tga tag aattcattga tcataatcag		734	
Lys Ser Phe Asn Arg Gly Glu Cys Xaa Xaa
            210                 215
ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa	794
cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 	854
ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc	914
tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggcggc cgctgaggcg	974
cgcctactga gtcattaggg actttccaat gggttttgcc cagtacataa ggtcaatagg	1034
ggtgaatcaa caggaaagtc ccattggagc caagtacact gagtcaatag ggactttcca	1094
ttgggttttg cccagtacaa aaggtcaata gggggtgagt caatgggttt ttcccattat	1154
tggcacgtac ataaggtcaa taggggtgag tcattgggtt tttccagcca atttaattaa	1214
aacgccatgt actttcccac cattgacgtc aatgggctat tgaaactaat gcaacgtgac	1274
ctttaaacgg tactttccca tagctgatta atgggaaagt accgttctcg agccaataca	1334
cgtcaatggg aagtgaaagg gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa	1394
ttccatattg gcacgcattc tattggctga gctgcgttct acgtgggtat aagaggcgcg	1454
accagcgtcg gtaccgtcgc agtcttcggt ctgaccaccg tagaacgcag cctcaggacc	1514
tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc	1574
ggattccccg tgccaagagt gacgtaagta ccgcctatag agtctatagg cccaccccct	1634
tggcttctta tgcatgctat actgtttttg gcttggggtc tatacacccc cgcttcctca	1694
tgttataggt gatggtatag cttagcctat aggtgtgggt tattgaccat tattgaccac	1754
tcccctattg gtgacgatac tttccattac taatccataa catggctctt tgccacaact	1814
ctctttattg gctatatgcc aatacactgt ccttcagaga ctgacacgga ctctgtattt	1874
ttacaggatg gggtctcatt tattatttac aaattcacat atacaacacc accgtcccca	1934
gtgcccgcag tttttattaa acataacgtg ggatctccac gcgaatctcg ggtacgtgtt	1994
ccggacatgg gctcttctcc ggtagcggcg gagcttctac atccgagccc tgctcccatg	2054
cctccagcga ctcatggtcg ctcggcagct ccttgctcct aacagtggag gccagactta	2114
ggcacagcac gatgcccacc accaccagtg tgccgcacaa ggccgtggcg gtagggtatg	2174
tgtctgaaaa tgagctcggg gagcgggctt gcaccgctga cgcatttgga agacttaagg	2234
cagcggcaga agaagatgca ggcagctgag ttgttgtgtt ctgataagag tcagaggtaa	2294
ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta ctcgttgctg	2354
ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt tccatgggtc	2414
ttttctgcag tcaccgtcct tgacacggga tccgccgcca ccatgatgcg gcctatcgtg	2474
ctggtgctgc tgttcgccac aagcgctctg gct cag gtg cag ctg cag cag agc 	2528
                                     Gln Val Gln Leu Gln Gln Ser
                                     1               5
gac gcc gag ctg gtg aag cct ggc gct agc gtg aag atc agc tgc aag 	2576
Asp Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys
        10                  15                  20
gcc agc ggc tac acc ttc acc gat cac gcc atc cac tgg gct aag cag 	2624
Ala Ser Gly Tyr Thr Phe Thr Asp His Ala Ile His Trp Ala Lys Gln
    25                  30                  35 
aag ccc gag cag ggc ctg gag tgg atc ggc tac atc agc ccc ggc aac 	2672
Lys pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr Ile Ser Pro Gly Asn
40                  45                  50                  55
gac gac atc aag tac aac gag aag ttc aag ggc aag gcc acc ctg acc		2720
Asp Asp Ile Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr
                60                  65                  70
gcc gac aag agc agc agc acc gcc tac atg cag ctg aac agc ctg acc 	2768
Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr
            75                  80                  85
agc gag gac agc gcc gtg tac ttc tgc aag cgg agc tac tac ggc cac 	2816
Ser Glu Asp Ser Ala Val Tyr Phe Cys Lys Arg Ser Tyr Tyr Gly His
        90                  95                  100
tgg ggc cag ggc acc acc ctg aca gtg agc agc gct agc acc aag ggc 	2864
Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ala Ser Thr Lys Gly
    105                 110                 115
cca agc gtg ttc cca ctg gcc ccc tgc agc aga agc acc agc gag agc 	2912
Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser
120                 125                 130                 135
aca gcc gcc ctg ggc tgc ctg gtg aag gac tac ttc ccc gag ccc gtg 	2960
Thr Ala Ala Leu Gly Cys Leu Val Lys Glu Tyr Phe Pro Glu Pro Val
                140                 145                 150
acc gtg tcc tgg aac agc gga gcc ctg aca agc gga gtg cac acc ttc 	3008
Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe
            155                 160                 165
ccc gcc gtg ctg cag agc agc ggc ctg tac tcc ctg agc agc gtg gtg		3056
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val
        170                 175                 180
acc gtg cca agc agc agc ctg ggc acc aag acc tac acc tgc aac gtg 	3104
Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr cys Asn Val
    185                 190                 195
gac cac aag ccc agc aac acc aaa gtg gac aag cgc gtg gag agc aag 	3152
Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys
200                 205                 210                 215
tac ggc cct ccc tgc ccc agc tgt ccc gcc cca gag ttc ctg ggc gga 	3200
Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Glu Leu Gly Gly
                220                 225                 230          
ccc tca gtg ttt ctg ttc cca ccc aag ccc aag gat acc ctg atg atc 	3248
Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
            235                 240                 245
agc cgg acc cct gaa gtg acc tgc gtg gtg gtg gat gtg agc cag gag 	3296
Ser Arg Thr Pro Glu val Thr Cys Val Val Val Asp Val Ser Gln Glu
        250                 255                 260
gac ccc gaa gtc cag ttc aat tgg tac gtg gac ggc gtg gaa gtg cac 	3344
Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
    265                 270                 275
aac gcc aag acc aag ccc aga gag gag cag ttc aac agc acc tac cgc 	3392
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
280                 285                 290                 295
gtg gtg tct gtg ctg acc gtg ctg cac cag gat tgg ctg aac ggc aaa 	3440
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
                300                 305                 310
gag tac aag tgc aag gtc tcc aac aag ggc ctg cct agc agc atc gag 	3488
Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
            315                 320                 325
aaa acc atc agc aag gcc aag ggc cag cca cgc gag ccc cag gtg tac 	3536
Lys Thr Ile Ser Lys Ala lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
        330                 335                 340
acc ctg ccc ccc agc caa gag gag atg acc aag aac cag gtg tcc ctg 	3584
Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
    345                 350                 355
acc tgt ctg gtg aag ggc ttc tac ccc agc gac atc gcc gtg gag tgg 	3632
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
360                 365                 370                 375
gag agc aac ggc cag ccc gag aac aac tac aag acc acc ccc cct gtg 	3680
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr pro Pro Val
                380                 385                 390
ctg gac agc gat ggc agc ttc ttc ctg tac tca cgg ctg acc gtg gat 	3728
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
            395                 400                 405
aag agc aga tgg caa gag ggc aat gtc ttt agc tgc agc gtg atg cac 	3776
Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
        410                 415                 420
gag gcc ctg cac aat cct aca ccc aga aga gcc tga gcctgtcc		3820
Glu Ala Leu His Asn Pro Thr Pro Arg Arg Ala Xaa
    425                 430
cctgggcaag tgatagtcgc gaattgatca taatcagcca taccacattt gtagaggttt	3880
tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa	3940
ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca	4000
caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca	4060
tcaatgtatc ttatcatgtc tggatcagct tgagcagctg tggaatgtgt gtcagttagg	4120
gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta	4180
gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat	4240
gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac	4300
tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga	4360
ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg	4420
ctaccatgga gaagttacta ttccgaagtt cctattctct agaaagtata ggaacttctc	4480
gggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc	4540
gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc	4600
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg	4660
cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt	4720
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc	4780
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc	4840
cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag	4900
agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg	4960
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa	5020
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag	5080	
gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact	5140
cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa	5200
attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt	5260
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag	5320
ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca	5380
gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc	5440
agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt	5500
ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg	5560
ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca	5620
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg	5680
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca	5740
tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg	5800
tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct	5860
cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca	5920
tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca	5980
gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg	6040
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac	6100
ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt	6160
attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc	6220
cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat	6280
taacctataa aaataggcgt atcacgaggc cctgatggct ctttgcggca cccatcgttc	6340
gtaatgttcc gtggcaccga ggacaaccct caagagaaaa tgtaatcaca ctgggaagtt	6400
cctattccga agttcctatt cttcaaaagg tataggaact tcctgcagtg aataataaaa	6460
tgtgtgtttg tccgaaatac gcgcctactg agtcattagg gactttccaa tgggttttgc	6520
ccagtacata aggtcaatag gggtgaatca acaggaaagt cccattggag ccaagtacac	6580
tgagtcaata gggactttcc attgggtttt gcccagtaca aaaggtcaat agggggtgag	6640
tcaatgggtt tttcccatta ttggcacgta cataaggtca ataggggtga gtcattgggt	6700
ttttccagcc aatttaatta aaacgccatg tactttccca ccattgacgt caatgggcta	6760
ttgaaactaa tgcaacgtga cctttaaacg gtactttccc atagctgatt aatgggaaag	6820
taccgttctc gagccaatac acgtcaatgg gaagtgaaag ggcagccaaa acgtaacacc	6880
gccccggttt tcccctggaa attccatatt ggcacgcatt ctattggctg agctgcgttc	6940
tacgtgggta taagaggcgc gaccagcgtc ggtaccgtcg cagtcttcgg tctgaccacc	7000
gtagaacgca gcctcaggac ctccatagaa gacaccggga ccgatccagc ctccgcggcc	7060
gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata	7120
gagtctatag gcccaccccc ttggcttctt atgcatgcta tactgttttt ggcttggggt	7180
ctatacaccc ccgcttcctc atgttatagg tgatggtata gcttagccta taggtgtggg	7240
ttattgacca ttattgacca ctcccctatt ggtgacgata ctttccatta ctaatccata	7300
acatggctct ttgccacaac tctctttatt ggctatatgc caatacactg tccttcagag	7360
actgacacgg actctgtatt tttacaggat ggggtctcat ttattattta caaattcaca	7420
tatacaacac caccgtcccc agtgcccgca gtttttatta aacataacgt gggatctcca	7480
cgcgaatctc gggtacgtgt tccggacatg ggctcttctc cggtagcggc ggagcttcta	7540
catccgagcc ctgctcccat gcctccagcg actcatggtc gctcggcagc tccttgctcc	7600
taacagtgga ggccagactt aggcacagca cgatgcccac caccaccagt gtgccgcaca	7660
aggccgtggc ggtagggtat gtgtctgaaa atgagctcgg ggagcgggct tgcaccgctg	7720
acgcatttgg aagacttaag gcagcggcag aagaagatgc aggcagctga gttgttgtgt	7780
tctgataaga gtcagaggta actcccgttg cggtgctgtt aacggtggag ggcagtgtag	7840
tctgagcagt actcgttgct gccgcgcgcg ccaccagaca taatagctga cagactaaca	7900
gactgttcct ttccatgggt cttttctgca gtcaccgtcc ttgacacg			7948			

<210>	3
<211>	492
<212>	DNA
<213>	artificial sequence

<220>
<223>	fragment of the mCMV IE1 promoter sequence

<400>	3

tactgagtca ttagggactt tccaatgggt tttgcccagt acataaggtc aataggggtg	60
aatcaacagg aaagtcccat tggagccaag tacactgagt caatagggac tttccattgg	120
gttttgccca gtacaaaagg tcaatagggg gtgagtcaat gggtttttcc cattattggc	180
acgtacataa ggtcaatagg ggtgagtcat tgggtttttc cagccaattt aattaaaacg	240
ccatgtactt tcccaccatt gacgtcaatg ggctattgaa actaatgcaa cgtgaccttt	300
aaacggtact ttcccatagc tgattaatgg gaaagtaccg ttctcgagcc aatacacgtc	360
aatgggaagt gaaagggcag ccaaaacgta acaccgcccc ggttttcccc tggaaattcc	420
atattggcac gcattctatt ggctgagctg cgttctacgt gggtataaga ggcgcgacca	480
gcgtcggtac cg								492

<210>	4
<211>	102
<212>	DNA
<213>	artificial sequence

<220>
<223>	fragment of the mCMV IE1 promoter sequence ("core promoter")

<400>	4

acaccgcccc ggttttcccc tggaaattcc atattggcac gcattctatt ggctgagctg	60
cgttctacgt gggtataaga ggcgcgacca gcgtcggtac cg				102

<210>	5
<211>	117
<212>	DNA
<213>	artificial sequence

<220>
<223>	fragment of the hCMV IE1 promoter sequence ("core promoter")

<400>	5

gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat  	 60
gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccg     	 117


<210>	6
<211>	452
<212>	DNA
<213>	artificial sequence

<220>
<223>	fragment of the sCMV IE1 enhancer sequence

<400>	6

gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg	60
gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg	120
gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg	180
gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt	240
ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg	300
gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta	360
ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt	420
tcctattgac gtagatagcc cctcccaatg gg					452

<210>	7
<211>	1074
<212>	DNA
<213>	artificial sequence

<220>
<223>	chimeric 5'-untranslated regulatory sequence ("construct 1")

<220>
<221>	enhancer
<222>	(1)...(582)
<223>	fragment of hCMV IE1 and mCMV IE1 enhancer sequence

<220>
<221>	promoter
<222>	(583)...(1074)
<223>	fragment of mCMV IE1 promoter sequence

<400>	7

ctgcagtgaa taataaaatg tgtgtttgtc cgaaatacgc gttttgagat ttctgtcgcc	60
gactaaattc atgtcgcgcg atagtggtgt ttatcgccga tagagatggc gatattggaa	120
aaatcgatat ttgaaaatat ggcatattga aaatgtcgcc gatgtgagtt tctgtgtaac	180
tgatatcgcc atttttccaa aagtgatttt tgggcatacg cgatatctgg cgatagcgct	240
tatatcgttt acgggggatg gcgatagacg actttggtga cttgggcgat tctgtgtgtc	300
gcaaatatcg cagtttcgat ataggtgaca gacgatatga ggctatatcg ccgatagagg	360
cgacatcaag ctggcacatg gccaatgcat atcgatctat acattgaatc aatattggcc	420
attagccata ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca	480
tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc	540
atgttgacat tgattattga ctagttatta atagtaatca attactgagt cattagggac	600
tttccaatgg gttttgccca gtacataagg tcaatagggg tgaatcaaca ggaaagtccc	660
attggagcca agtacactga gtcaataggg actttccatt gggttttgcc cagtacaaaa	720
ggtcaatagg gggtgagtca atgggttttt cccattattg gcacgtacat aaggtcaata	780
ggggtgagtc attgggtttt tccagccaat ttaattaaaa cgccatgtac tttcccacca	840
ttgacgtcaa tgggctattg aaactaatgc aacgtgacct ttaaacggta ctttcccata	900
gctgattaat gggaaagtac cgttctcgag ccaatacacg tcaatgggaa gtgaaagggc	960
agccaaaacg taacaccgcc ccggttttcc cctggaaatt ccatattggc acgcattcta	1020
ttggctgagc tgcgttctac gtgggtataa gaggcgcgac cagcgtcggt accg		1074

<210>	8
<211>	1128
<212>	DNA
<213>	artificial sequence

<220>
<223>	chimeric 5'-untranslated regulatory sequence ("construct 2")

<220>
<221>	enhancer
<222>	(1)...(1026)
<223>	fragment of hCMV IE1 enhancer sequence

<220>
<221>	promoter
<222>	(1027)...(1128)
<223>	fragment of mCMV IE1 promoter sequence ("core promoter")

<400>	8

ctgcagtgaa taataaaatg tgtgtttgtc cgaaatacgc gttttgagat ttctgtcgcc	60
gactaaattc atgtcgcgcg atagtggtgt ttatcgccga tagagatggc gatattggaa	120
aaatcgatat ttgaaaatat ggcatattga aaatgtcgcc gatgtgagtt tctgtgtaac	180
tgatatcgcc atttttccaa aagtgatttt tgggcatacg cgatatctgg cgatagcgct	240
tatatcgttt acgggggatg gcgatagacg actttggtga cttgggcgat tctgtgtgtc	300
gcaaatatcg cagtttcgat ataggtgaca gacgatatga ggctatatcg ccgatagagg	360
cgacatcaag ctggcacatg gccaatgcat atcgatctat acattgaatc aatattggcc	420
attagccata ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca	480
tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc	540
atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca	600
tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc	660
gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat	720
agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt	780
acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc	840
cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta	900
cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg	960
atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt	1020
gttttgacac cgccccggtt ttcccctgga aattccatat tggcacgcat tctattggct	1080
gagctgcgtt ctacgtgggt ataagaggcg cgaccagcgt cggtaccg			1128

<210>	9
<211>	509
<212>	DNA
<213>	artificial sequence

<220>
<223>	chimeric 5'-untranslated regulatory sequence ("construct 3")

<220>
<221>	enhancer
<222>	(1)...(407)
<223>	fragment of hCMV IE1 enhancer sequence

<220>
<221>	promoter
<222>	(408)...(509)
<223>	fragment of mCMV IE1 promoter sequence ("core promoter")

<400>	9

cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca	60
ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt	120
caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg	180
ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag	240
tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt	300
accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg	360
ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttgaca ccgccccggt	420
tttcccctgg aaattccata ttggcacgca ttctattggc tgagctgcgt tctacgtggg	480
tataagaggc gcgaccagcg tcggtaccg						509

<210>	10
<211>	961
<212>	DNA
<213>	artificial sequence

<220>
<223>	chimeric 5'-untranslated regulatory sequence ("construct 4")

<220>
<221>	enhancer
<222>	(1)...(452)
<223>	fragment of sCMV IE1 enhancer sequence

<220>
<221>	enhancer
<222>	(453)...(859)
<223>	fragment of hCMV IE1 enhancer sequence

<220>
<221>	promoter
<222>	(860)...(961)
<223>	fragment of mCMV IE1 promoter sequence ("core promoter")

<400>	10

gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg	60
gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg	120
gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg	180
gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt	240
ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg	300
gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta	360
ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt	420
tcctattgac gtagatagcc cctcccaatg ggcgcgttac ataacttacg gtaaatggcc	480
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca	540
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg	600
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg	660
acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt	720
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca	780
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg	840
tcaatgggag tttgttttga caccgccccg gttttcccct ggaaattcca tattggcacg	900
cattctattg gctgagctgc gttctacgtg ggtataagag gcgcgaccag cgtcggtacc	960
g									961

<210>	11
<211>	909
<212>	DNA
<213>	artificial sequence

<220>
<223>	chimeric 5'-untranslated regulatory sequence ("construct 5")

<220>
<221>	enhancer
<222>	(1)...(807)
<223>	fragment of sCMV IE1 enhancer sequence

<220>
<221>	promoter
<222>	(808)...(909)
<223>	fragment of mCMV IE1 promoter sequence ("core promoter")

<400>	11

gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg	60
gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg	120
gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg	180
gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt	240
ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg	300
gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta	360
ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt	420
tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc	480
ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc	540
tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat	600
tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg	660
acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca	720
atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt	780
acattactat tggaagtacg ccagggtaca ccgccccggt tttcccctgg aaattccata	840
ttggcacgca ttctattggc tgagctgcgt tctacgtggg tataagaggc gcgaccagcg	900
tcggtaccg								909

<210>	12
<211>	976
<212>	DNA
<213>	artificial sequence

<220>
<223>	chimeric 5'-untranslated regulatory sequence ("construct 6")

<220>
<221>	enhancer
<222>	(1)...(452)
<223>	fragment of sCMV IE1 enhancer sequence

<220>
<221>	enhancer
<222>	(453)…(859)
<223>	fragment of hCMV IE1 enhancer sequence

<220>
<221>	promoter
<222>	(860)...(976)
<223>	fragment of hCMV IE1 promoter sequence ("core promoter")

<400>	12
gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg  	60
gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg  	120
gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg   	180
gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt   	240
ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg   	300
gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta   	360
ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt   	420
tcctattgac gtagatagcc cctcccaatg ggcgcgttac ataacttacg gtaaatggcc   	480
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca   	540
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg  	600 
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg  	660
acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt   	720
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca  	780
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg  	840
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact   	900
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag   	960
ctcgtttagt gaaccg                                                   	976

<210>	13
<211>	924
<212>	DNA
<213>	artificial sequence

<220>
<223>	chimeric 5'-untranslated regulatory sequence ("construct 7")

<220>
<221>	enhancer
<222>	(1)...(807)
<223>	fragment of sCMV IE1 enhancer sequence

<220>
<221>	promoter
<222>	(808)...(924)
<223>	fragment of hCMV IE1 promoter sequence ("core promoter")

<400>	13
gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg   	60
gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg  	120
gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg   	180
gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt   	240
ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg   	300
gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta   	360
ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt   	420
tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc   	480
ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc   	540
tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat   	600
tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg   	660
acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca   	720
atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt   	780
acattactat tggaagtacg ccagggtgca ccaaaatcaa cgggactttc caaaatgtcg   	840
taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat   	900
aagcagagct cgtttagtga accg                                          	924

<210>	14
<211>	214
<212>	PRT
<213>	artificial sequence

<220>
<223>	optimized cB72.3 kappa light chain

<400>	14

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Val Ser Val Gly 
1               5                   10                  15
Asp Thr Val Thr Ile Thr Cys Arg Ala Ser Glu Asn Ile Tyr Ser Asn
            20                  25                  30
Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val
        35                  40                  45
Tyr Ala Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60
Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn Ser Leu Gln Ser
65                  70                  75                  80
Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp Gly Thr Pro Tyr 
                85                  90                  95
Thr Phe Gly Gly Gly Thr Arg Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110
Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125
Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala
    130                 135                 140
Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160
Asp Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser
                165                 170                 175
Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr
            180                 185                 190
Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205
Phe Asn Arg Gly Glu Cys 
    210 

<210>	15
<211>	434
<212>	PRT
<213>	artificial sequence

<220>
<223>	optimized cB72.3 gamma-4 heavy chain

<400>	15

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15
Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His
            20                  25                  30
Ala Ile His Trp Ala Lys Gln Lys pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45
Gly Tyr Ile Ser Pro Gly Asn Asp Asp Ile Lys Tyr Asn Glu Lys Phe 
    50                  55                  60
Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80
Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys
                85                  90                  95
Lys Arg Ser Tyr Tyr Gly His Trp Gly Gln Gly Thr Thr Leu Thr Val 
            100                 105                 110
Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys
        115                 120                 125
Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys
    130                 135                 140
Glu Tyr Phe Pro Glu Pro Val Thr val Ser Trp Asn Ser Gly Ala Leu
145                 150                 155                 160
Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu
                165                 170                 175
Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
            180                 185                 190
Lys Thr Tyr Thr cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val
        195                 200                 205
Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
    210                 215                 220
Ala Pro Glu Glu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
225                 230                 235                 240
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu val Thr Cys Val 
                245                 250                 255
Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr
            260                 265                  270
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
        275                 280                 285
Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
    290                 295                 300
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
305                 310                 315                 320
Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
                325                 330                 335
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met
            340                 345                 350
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
        355                 360                 365
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
    370                 375                 380 
Tyr Lys Thr Thr pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu
385                 390                 395                 400
Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val
                405                 410                 415
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Pro Thr Pro Arg
            420                 425                 430
Arg Ala