                         SEQUENCE LISTING

<110>  CEDARS-SINAI MEDICAL CENTER
       PIMENTEL, Mark
       CHANG, Christopher
 
<120>  DIAGNOSIS AND TREATMENT OF MOTILITY DISORDERS OF THE GUT AND 
       BLADDER, AND OF FIBROMYALGIA

<130>  065472-000379WO00

<150>  61/701,923
<151>  2012-09-17

<150>  61/762,632
<151>  2013-02-08

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1066
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Val Phe His Thr Arg Thr Ile Glu Ser Ile Leu Glu Pro Val 
1               5                   10                  15      


Ala Gln Gln Ile Ser His Leu Val Ile Met His Glu Glu Gly Glu Val 
            20                  25                  30          


Asp Gly Lys Ala Ile Pro Asp Leu Thr Ala Pro Val Ala Ala Val Gln 
        35                  40                  45              


Ala Ala Val Ser Asn Leu Val Arg Val Gly Lys Glu Thr Val Gln Thr 
    50                  55                  60                  


Thr Glu Asp Gln Ile Leu Lys Arg Asp Met Pro Pro Ala Phe Ile Lys 
65                  70                  75                  80  


Val Glu Asn Ala Cys Thr Lys Leu Val Gln Ala Ala Gln Met Leu Gln 
                85                  90                  95      


Ser Asp Pro Tyr Ser Val Pro Ala Arg Asp Tyr Leu Ile Asp Gly Ser 
            100                 105                 110         


Arg Gly Ile Leu Ser Gly Thr Ser Asp Leu Leu Leu Thr Phe Asp Glu 
        115                 120                 125             


Ala Glu Val Arg Lys Ile Ile Arg Val Cys Lys Gly Ile Leu Glu Tyr 
    130                 135                 140                 


Leu Thr Val Ala Glu Val Val Glu Thr Met Glu Asp Leu Val Thr Tyr 
145                 150                 155                 160 


Thr Lys Asn Leu Gly Pro Gly Met Thr Lys Met Ala Lys Met Ile Asp 
                165                 170                 175     


Glu Arg Gln Gln Glu Leu Thr His Gln Glu His Arg Val Met Leu Val 
            180                 185                 190         


Asn Ser Met Asn Thr Val Lys Glu Leu Leu Pro Val Leu Ile Ser Ala 
        195                 200                 205             


Met Lys Ile Phe Val Thr Thr Lys Asn Ser Lys Asn Gln Gly Ile Glu 
    210                 215                 220                 


Glu Ala Leu Lys Asn Arg Asn Phe Thr Val Glu Lys Met Ser Ala Glu 
225                 230                 235                 240 


Ile Asn Glu Ile Ile Arg Val Leu Gln Leu Thr Ser Trp Asp Glu Asp 
                245                 250                 255     


Ala Trp Ala Ser Lys Asp Thr Glu Ala Met Lys Arg Ala Leu Ala Ser 
            260                 265                 270         


Ile Asp Ser Lys Leu Asn Gln Ala Lys Gly Trp Leu Arg Asp Pro Ser 
        275                 280                 285             


Ala Ser Pro Gly Asp Ala Gly Glu Gln Ala Ile Arg Gln Ile Leu Asp 
    290                 295                 300                 


Glu Ala Gly Lys Val Gly Glu Leu Cys Ala Gly Lys Glu Arg Arg Glu 
305                 310                 315                 320 


Ile Leu Gly Thr Cys Lys Met Leu Gly Gln Met Thr Asp Gln Val Ala 
                325                 330                 335     


Asp Leu Arg Ala Arg Gly Gln Gly Ser Ser Pro Val Ala Met Gln Lys 
            340                 345                 350         


Ala Gln Gln Val Ser Gln Gly Leu Asp Val Leu Thr Ala Lys Val Glu 
        355                 360                 365             


Asn Ala Ala Arg Lys Leu Glu Ala Met Thr Asn Ser Lys Gln Ser Ile 
    370                 375                 380                 


Ala Lys Lys Ile Asp Ala Ala Gln Asn Trp Leu Ala Asp Pro Asn Gly 
385                 390                 395                 400 


Gly Pro Glu Gly Glu Glu Gln Ile Arg Gly Ala Leu Ala Glu Ala Arg 
                405                 410                 415     


Lys Ile Ala Glu Leu Cys Asp Asp Pro Lys Glu Arg Asp Asp Ile Leu 
            420                 425                 430         


Arg Ser Leu Gly Glu Ile Ser Ala Leu Thr Ser Lys Leu Ala Asp Leu 
        435                 440                 445             


Arg Arg Gln Gly Lys Gly Asp Ser Pro Glu Ala Arg Ala Leu Ala Lys 
    450                 455                 460                 


Gln Val Ala Thr Ala Leu Gln Asn Leu Gln Thr Lys Thr Asn Arg Ala 
465                 470                 475                 480 


Val Ala Asn Ser Arg Pro Ala Lys Ala Ala Val His Leu Glu Gly Lys 
                485                 490                 495     


Ile Glu Gln Ala Gln Arg Trp Ile Asp Asn Pro Thr Val Asp Asp Arg 
            500                 505                 510         


Gly Val Gly Gln Ala Ala Ile Arg Gly Leu Val Ala Glu Gly His Arg 
        515                 520                 525             


Leu Ala Asn Val Met Met Gly Pro Tyr Arg Gln Asp Leu Leu Ala Lys 
    530                 535                 540                 


Cys Asp Arg Val Asp Gln Leu Thr Ala Gln Leu Ala Asp Leu Ala Ala 
545                 550                 555                 560 


Arg Gly Glu Gly Glu Ser Pro Gln Ala Arg Ala Leu Ala Ser Gln Leu 
                565                 570                 575     


Gln Asp Ser Leu Lys Asp Leu Lys Ala Arg Met Gln Glu Ala Met Thr 
            580                 585                 590         


Gln Glu Val Ser Asp Val Phe Ser Asp Thr Thr Thr Pro Ile Lys Leu 
        595                 600                 605             


Leu Ala Val Ala Ala Thr Ala Pro Pro Asp Ala Pro Asn Arg Glu Glu 
    610                 615                 620                 


Val Phe Asp Glu Arg Ala Ala Asn Phe Glu Asn His Ser Gly Lys Leu 
625                 630                 635                 640 


Gly Ala Thr Ala Glu Lys Ala Ala Ala Val Gly Thr Ala Asn Lys Ser 
                645                 650                 655     


Thr Val Glu Gly Ile Gln Ala Ser Val Lys Thr Ala Arg Glu Leu Thr 
            660                 665                 670         


Pro Gln Val Val Ser Ala Ala Arg Ile Leu Leu Arg Asn Pro Gly Asn 
        675                 680                 685             


Gln Ala Ala Tyr Glu His Phe Glu Thr Met Lys Asn Gln Trp Ile Asp 
    690                 695                 700                 


Asn Val Glu Lys Met Thr Gly Leu Val Asp Glu Ala Ile Asp Thr Lys 
705                 710                 715                 720 


Ser Leu Leu Asp Ala Ser Glu Glu Ala Ile Lys Lys Asp Leu Asp Lys 
                725                 730                 735     


Cys Lys Val Ala Met Ala Asn Ile Gln Pro Gln Met Leu Val Ala Gly 
            740                 745                 750         


Ala Thr Ser Ile Ala Arg Arg Ala Asn Arg Ile Leu Leu Val Ala Lys 
        755                 760                 765             


Arg Glu Val Glu Asn Ser Glu Asp Pro Lys Phe Arg Glu Ala Val Lys 
    770                 775                 780                 


Ala Ala Ser Asp Glu Leu Ser Lys Thr Ile Ser Pro Met Val Met Asp 
785                 790                 795                 800 


Ala Lys Ala Val Ala Gly Asn Ile Ser Asp Pro Gly Leu Gln Lys Ser 
                805                 810                 815     


Phe Leu Asp Ser Gly Tyr Arg Ile Leu Gly Ala Val Ala Lys Val Arg 
            820                 825                 830         


Glu Ala Phe Gln Pro Gln Glu Pro Asp Phe Pro Pro Pro Pro Pro Asp 
        835                 840                 845             


Leu Glu Gln Leu Arg Leu Thr Asp Glu Leu Ala Pro Pro Lys Pro Pro 
    850                 855                 860                 


Leu Pro Glu Gly Glu Val Pro Pro Pro Arg Pro Pro Pro Pro Glu Glu 
865                 870                 875                 880 


Lys Asp Glu Glu Phe Pro Glu Gln Lys Ala Gly Glu Val Ile Asn Gln 
                885                 890                 895     


Pro Met Met Met Ala Ala Arg Gln Leu His Asp Glu Ala Arg Lys Trp 
            900                 905                 910         


Ser Ser Lys Gly Asn Asp Ile Ile Ala Ala Ala Lys Arg Met Ala Leu 
        915                 920                 925             


Leu Met Ala Glu Met Ser Arg Leu Val Arg Gly Gly Ser Gly Thr Lys 
    930                 935                 940                 


Arg Ala Leu Ile Gln Cys Ala Lys Asp Ile Ala Lys Ala Ser Asp Glu 
945                 950                 955                 960 


Val Thr Arg Leu Ala Lys Glu Val Ala Lys Gln Cys Thr Asp Lys Arg 
                965                 970                 975     


Ile Arg Thr Asn Leu Leu Gln Val Cys Glu Arg Ile Pro Thr Ile Ser 
            980                 985                 990         


Thr Gln Leu Lys Ile Leu Ser Thr  Val Lys Ala Thr Met  Leu Gly Arg 
        995                 1000                 1005             


Thr Asn  Ile Ser Asp Glu Glu  Ser Glu Gln Ala Thr  Glu Met Leu 
    1010                 1015                 1020             


Val His  Asn Ala Gln Asn Leu  Met Gln Ser Val Lys  Glu Thr Val 
    1025                 1030                 1035             


Arg Glu  Ala Glu Ala Ala Ser  Ile Lys Ile Arg Thr  Asp Ala Gly 
    1040                 1045                 1050             


Phe Thr  Leu Arg Trp Val Arg  Lys Thr Pro Trp Tyr  Gln 
    1055                 1060                 1065     


<210>  2
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  2
ggagattact gccctggctc cta                                               23


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  3
gactcatcgt actcctgctt gctg                                              24


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  4
gccaagcagt gcacagataa                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  5
tctttctaac ccagcgcagt                                                   20


<210>  6
<211>  633
<212>  PRT
<213>  Shigella

<400>  6

Met His Asn Val Asn Asn Thr Gln Ala Pro Thr Phe Leu Tyr Lys Ala 
1               5                   10                  15      


Thr Ser Pro Ser Ser Thr Glu Tyr Ser Glu Leu Lys Ser Lys Ile Ser 
            20                  25                  30          


Asp Ile His Ser Ser Gln Thr Ser Leu Lys Thr Pro Ala Ser Val Ser 
        35                  40                  45              


Glu Lys Glu Asn Phe Ala Thr Ser Phe Asn Gln Lys Cys Leu Asp Phe 
    50                  55                  60                  


Leu Phe Ser Ser Ser Gly Lys Glu Asp Val Leu Arg Ser Ile Tyr Ser 
65                  70                  75                  80  


Asn Ser Met Asn Ala Tyr Ala Lys Ser Glu Ile Leu Glu Phe Ser Asn 
                85                  90                  95      


Val Leu Tyr Ser Leu Val His Gln Asn Gly Leu Asn Phe Glu Asn Glu 
            100                 105                 110         


Lys Gly Leu Gln Lys Ile Val Ala Gln Tyr Ser Glu Leu Ile Ile Lys 
        115                 120                 125             


Asp Lys Leu Ser Gln Asp Ser Ala Phe Gly Pro Trp Ser Ala Lys Asn 
    130                 135                 140                 


Lys Lys Leu His Gln Leu Arg Gln Asn Ile Glu His Arg Leu Ala Leu 
145                 150                 155                 160 


Leu Ala Gln Gln His Thr Ser Gly Glu Ala Leu Ser Leu Gly Gln Lys 
                165                 170                 175     


Leu Leu Asn Thr Glu Val Ser Ser Phe Ile Lys Asn Asn Ile Leu Ala 
            180                 185                 190         


Glu Leu Lys Leu Ser Asn Glu Thr Val Ser Ser Leu Lys Leu Asp Asp 
        195                 200                 205             


Leu Val Asp Ala Gln Ala Lys Leu Ala Phe Asp Ser Leu Arg Asn Gln 
    210                 215                 220                 


Arg Lys Asn Thr Ile Asp Ser Lys Gly Phe Gly Ile Gly Lys Leu Ser 
225                 230                 235                 240 


Arg Asp Leu Asn Thr Val Ala Val Phe Pro Glu Leu Leu Arg Lys Val 
                245                 250                 255     


Leu Asn Asp Ile Leu Glu Asp Ile Lys Asp Ser His Pro Ile Gln Asp 
            260                 265                 270         


Gly Leu Pro Thr Pro Pro Glu Asp Met Pro Asp Gly Gly Pro Thr Pro 
        275                 280                 285             


Gly Ala Asn Glu Lys Thr Ser Gln Pro Val Ile His Tyr His Ile Asn 
    290                 295                 300                 


Asn Asp Asn Arg Thr Tyr Asp Asn Arg Val Phe Asp Asn Arg Val Tyr 
305                 310                 315                 320 


Asp Asn Ser Tyr His Glu Asn Pro Glu Asn Asp Ala Gln Ser Pro Thr 
                325                 330                 335     


Ser Gln Thr Asn Asp Leu Leu Ser Arg Asn Gly Asn Ser Leu Leu Asn 
            340                 345                 350         


Pro Gln Arg Ala Leu Val Gln Lys Val Thr Ser Val Leu Pro His Ser 
        355                 360                 365             


Ile Ser Asp Thr Val Gln Thr Phe Ala Asn Asn Ser Ala Leu Glu Lys 
    370                 375                 380                 


Val Phe Asn His Thr Pro Asp Asn Ser Asp Gly Ile Gly Ser Asp Leu 
385                 390                 395                 400 


Leu Thr Thr Ser Ser Gln Glu Arg Ser Ala Asn Asn Ser Leu Ser Arg 
                405                 410                 415     


Gly His Arg Pro Leu Asn Ile Gln Asn Ser Ser Thr Thr Pro Pro Leu 
            420                 425                 430         


His Pro Glu Gly Val Thr Ser Ser Asn Asp Asn Ser Ser Asp Thr Thr 
        435                 440                 445             


Lys Ser Ser Ala Ser Leu Ser His Arg Val Ala Ser Gln Ile Asn Lys 
    450                 455                 460                 


Phe Asn Ser Asn Thr Asp Ser Lys Val Leu Gln Thr Asp Phe Leu Ser 
465                 470                 475                 480 


Arg Asn Gly Asp Thr Tyr Leu Thr Arg Glu Thr Ile Phe Glu Ala Ser 
                485                 490                 495     


Lys Lys Val Thr Asn Ser Leu Ser Asn Leu Ile Ser Leu Ile Gly Thr 
            500                 505                 510         


Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile 
        515                 520                 525             


Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys Val Thr 
    530                 535                 540                 


Asp Ala Asn Ile Arg Asn Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile 
545                 550                 555                 560 


Asp Lys Asn His Ala Ile Tyr Glu Lys Ala Lys Glu Val Ser Ser Ala 
                565                 570                 575     


Leu Ser Lys Val Leu Ser Lys Ile Asp Asp Thr Ser Ala Glu Leu Leu 
            580                 585                 590         


Thr Asp Asp Ile Ser Asp Leu Lys Asn Asn Asn Asp Ile Thr Ala Glu 
        595                 600                 605             


Asn Asn Asn Ile Tyr Lys Ala Ala Lys Asp Val Thr Thr Ser Leu Ser 
    610                 615                 620                 


Lys Val Leu Lys Asn Ile Asn Lys Asp 
625                 630             


