                         SEQUENCE LISTING

<110>  Centre for Bio-Separation Technology
 
<120>  METHODS FOR PRODUCING RECOMBINANT PEPTIDES AND PROTEIN FROM 
       NON-FILAMENTOUS FUNGI AND RECOMBINANT HOST CELL THEREOF

<130>  IP20617/VJ/as

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  2247
<212>  DNA
<213>  Homo sapiens


<220>
<221>  gene
<222>  (1)..(2247)

<400>  1
gaattcacgt ggcccagccg gccagccacc agaagatact acctgggtgc agtggaactg       60

tcatgggact atatgcaaag tgatctcggt gagctgcctg tggacgcaag atttcctcct      120

agagtgccaa aatcttttcc attcaacacc tcagtcgtgt acaaaaagac tctgtttgta      180

gaattcacgg atcacctttt caacatcgct aagccaaggc caccctggat gggtctgcta      240

ggtcctacca tccaggctga ggtttatgat acagtggtca ttacacttaa gaacatggct      300

tcccatcctg tcagtcttca tgctgttggt gtatcctact ggaaagcttc tgagggagct      360

gaatatgatg atcagaccag tcaaagggag aaagaagatg ataaagtctt ccctggtgga      420

agccatacat atgtctggca ggtcctgaaa gagaatggtc caatggcctc tgacccactg      480

tgccttacct actcatatct ttctcatgtg gacctggtaa aagacttgaa ttcaggcctc      540

attggagccc tactagtatg tagagaaggg agtctggcca aggaaaagac acagaccttg      600

cacaaattta tactactttt tgctgtattt gatgaaggga aaagttggca ctcagaaaca      660

aagaactcct tgatgcagga tagggatgct gcatctgctc gggcctggcc taaaatgcac      720

acagtcaatg gttatgtaaa caggtctctg ccaggtctga ttggatgcca caggaaatca      780

gtctattggc atgtgattgg aatgggcacc actcctgaag tgcactcaat attcctcgaa      840

ggtcacacat ttcttgtgag gaaccatcgc caggcgtcct tggaaatctc gccaataact      900

ttccttactg ctcaaacact cttgatggac cttggacagt ttctactgtt ttgtcatatc      960

tcttcccacc aacatgatgg catggaagct tatgtcaaag tagacagctg tccagaggaa     1020

ccccaactac gaatgaaaaa taatgaagaa gcggaagact atgatgatga tcttactgat     1080

tctgaaatgg atgtggtcag gtttgatgat gacaactctc cttcctttat ccaaattcgc     1140

tcagttgcca agaagcatcc taaaacttgg gtacattaca ttgctgctga agaggaggac     1200

tgggactatg ctcccttagt cctcgccccc gatgacagaa gttataaaag tcaatatttg     1260

aacaatggcc ctcagcggat tggtaggaag tacaaaaaag tccgatttat ggcatacaca     1320

gatgaaacct ttaagactcg tgaagctatt cagcatgaat caggaatctt gggaccttta     1380

ctttatgggg aagttggaga cacactgttg attatattta agaatcaagc aagcagacca     1440

tataacatct accctcacgg aatcactgat gtccgtcctt tgtattcaag gagattacca     1500

aaaggtgtaa aacatttgaa ggattttcca attctgccag gagaaatatt caaatataaa     1560

tggacagtga ctgtagaaga tgggccaact aaatcagatc ctcggtgcct gacccgctat     1620

tactctagtt tcgttaatat ggagagagat ctagcttcag gactcattgg ccctctcctc     1680

atctgctaca aagaatctgt agatcaaaga ggaaaccaga taatgtcaga caagaggaat     1740

gtcatcctgt tttctgtatt tgatgagaac cgaagctggt acctcacaga gaatatacaa     1800

cgctttctcc ccaatccagc tggagtgcag cttgaggatc cagagttcca agcctccaac     1860

atcatgcaca gcatcaatgg ctatgttttt gatagtttgc agttgtcagt ttgtttgcat     1920

gaggtggcat actggtacat tctaagcatt ggagcacaga ctgacttcct ttctgtcttc     1980

ttctctggat ataccttcaa acacaaaatg gtctatgaag acacactcac cctattccca     2040

ttctcaggag aaactgtctt catgtcgatg gaaaacccag gtctatggat tctggggtgc     2100

cacaactcag actttcggaa cagaggcatg accgccttac tgaaggtttc tagttgtgac     2160

aagaacactg gtgattatta cgaggacagt tatgaagata tttcagcata cttgctgagt     2220

aaaaacaatg ccattgaacc aagatga                                         2247


<210>  2
<211>  748
<212>  PRT
<213>  Homo sapiens


<220>
<221>  PEPTIDE
<222>  (1)..(748)

<400>  2

Glu Phe Thr Trp Pro Ser Arg Pro Ala Thr Arg Arg Tyr Tyr Leu Gly 
1               5                   10                  15      


Ala Val Glu Leu Ser Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu 
            20                  25                  30          


Pro Val Asp Ala Arg Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe 
        35                  40                  45              


Asn Thr Ser Val Val Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp 
    50                  55                  60                  


His Leu Phe Asn Ile Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu 
65                  70                  75                  80  


Gly Pro Thr Ile Gln Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu 
                85                  90                  95      


Lys Asn Met Ala Ser His Pro Val Ser Leu His Ala Val Gly Val Ser 
            100                 105                 110         


Tyr Trp Lys Ala Ser Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln 
        115                 120                 125             


Arg Glu Lys Glu Asp Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr 
    130                 135                 140                 


Val Trp Gln Val Leu Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu 
145                 150                 155                 160 


Cys Leu Thr Tyr Ser Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu 
                165                 170                 175     


Asn Ser Gly Leu Ile Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu 
            180                 185                 190         


Ala Lys Glu Lys Thr Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala 
        195                 200                 205             


Val Phe Asp Glu Gly Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu 
    210                 215                 220                 


Met Gln Asp Arg Asp Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His 
225                 230                 235                 240 


Thr Val Asn Gly Tyr Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys 
                245                 250                 255     


His Arg Lys Ser Val Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro 
            260                 265                 270         


Glu Val His Ser Ile Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn 
        275                 280                 285             


His Arg Gln Ala Ser Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala 
    290                 295                 300                 


Gln Thr Leu Leu Met Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile 
305                 310                 315                 320 


Ser Ser His Gln His Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser 
                325                 330                 335     


Cys Pro Glu Glu Pro Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu 
            340                 345                 350         


Asp Tyr Asp Asp Asp Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe 
        355                 360                 365             


Asp Asp Asp Asn Ser Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys 
    370                 375                 380                 


Lys His Pro Lys Thr Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp 
385                 390                 395                 400 


Trp Asp Tyr Ala Pro Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys 
                405                 410                 415     


Ser Gln Tyr Leu Asn Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys 
            420                 425                 430         


Lys Val Arg Phe Met Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu 
        435                 440                 445             


Ala Ile Gln His Glu Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu 
    450                 455                 460                 


Val Gly Asp Thr Leu Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro 
465                 470                 475                 480 


Tyr Asn Ile Tyr Pro His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser 
                485                 490                 495     


Arg Arg Leu Pro Lys Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu 
            500                 505                 510         


Pro Gly Glu Ile Phe Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly 
        515                 520                 525             


Pro Thr Lys Ser Asp Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe 
    530                 535                 540                 


Val Asn Met Glu Arg Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu 
545                 550                 555                 560 


Ile Cys Tyr Lys Glu Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser 
                565                 570                 575     


Asp Lys Arg Asn Val Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser 
            580                 585                 590         


Trp Tyr Leu Thr Glu Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly 
        595                 600                 605             


Val Gln Leu Glu Asp Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser 
    610                 615                 620                 


Ile Asn Gly Tyr Val Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His 
625                 630                 635                 640 


Glu Val Ala Tyr Trp Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe 
                645                 650                 655     


Leu Ser Val Phe Phe Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr 
            660                 665                 670         


Glu Asp Thr Leu Thr Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met 
        675                 680                 685             


Ser Met Glu Asn Pro Gly Leu Trp Ile Leu Gly Cys His Asn Ser Asp 
    690                 695                 700                 


Phe Arg Asn Arg Gly Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp 
705                 710                 715                 720 


Lys Asn Thr Gly Asp Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala 
                725                 730                 735     


Tyr Leu Leu Ser Lys Asn Asn Ala Ile Glu Pro Arg 
            740                 745             


<210>  3
<211>  2145
<212>  DNA
<213>  Homo sapiens


<220>
<221>  gene
<222>  (1)..(2145)

<400>  3
gaattcacgt ggcccagccg gccgtctcgg atcggtaccg aaataactcg tactactctt       60

cagtcagatc aagaggaaat tgactatgat gataccatat cagttgaaat gaagaaggaa      120

gattttgaca tttatgatga ggatgaaaat cagagccccc gcagctttca aaagaaaaca      180

cgacactatt ttattgctgc agtggagagg ctctgggatt atgggatgag tagctcccca      240

catgttctaa gaaacagggc tcagagtggc agtgtccctc agttcaagaa agttgttttc      300

caggaattta ctgatggctc ctttactcag cccttatacc gtggagaact aaatgaacat      360

ttgggactcc tggggccata tataagagca gaagttgaag ataatatcat ggtaactttc      420

agaaatcagg cctctcgtcc ctattccttc tattctagcc ttatttctta tgaggaagat      480

cagaggcaag gagcagaacc tagaaaaaac tttgtcaagc ctaatgaaac caaaacttac      540

ttttggaaag tgcaacatca tatggcaccc actaaagatg agtttgactg caaagcctgg      600

gcttatttct ctgatgttga cctggaaaaa gatgtgcact caggcctgat tggacccctt      660

ctggtctgcc acactaacac actgaaccct gctcatggga gacaagtgac agtacaggaa      720

tttgctctgt ttttcaccat ctttgatgag accaaaagct ggtacttcac tgaaaatatg      780

gaaagaaact gcagggctcc ctgcaatatc cagatggaag atcccacttt taaagagaat      840

tatcgcttcc atgcaatcaa tggctacata atggatacac tacctggctt agtaatggct      900

caggatcaaa ggattcgatg gtatctgctc agcatgggca gcaatgaaaa catccattct      960

attcatttca gtggacatgt gttcactgta cgaaaaaaag aggagtataa aatggcactg     1020

tacaatctct atccaggtgt ttttgagaca gtggaaatgt taccatccaa agctggaatt     1080

tggcgggtgg aatgccttat tggcgagcat ctacatgctg ggatgagcac actttttctg     1140

gtgtacagca ataagtgtca gactcccctg ggaatggctt ctggacacat tagagatttt     1200

cagattacag cttcaggaca atatggacag tgggccccaa agctggccag acttcattat     1260

tccggatcaa tcaatgcctg gagcaccaag gagccctttt cttggatcaa ggtggatctg     1320

ttggcaccaa tgattattca cggcatcaag acccagggtg cccgtcagaa gttctccagc     1380

ctctacatct ctcagtttat catcatgtat agtcttgatg ggaagaagtg gcagacttat     1440

cgaggaaatt ccactggaac cttaatggtc ttctttggca atgtggattc atctgggata     1500

aaacacaata tttttaaccc tccaattatt gctcgataca tccgtttgca cccaactcat     1560

tatagcattc gcagcactct tcgcatggag ttgatgggct gtgatttaaa tagttgcagc     1620

atgccattgg gaatggagag taaagcaata tcagatgcac agattactgc ttcatcctac     1680

tttaccaata tgtttgccac ctggtctcct tcaaaagctc gacttcacct ccaagggagg     1740

agtaatgcct ggagacctca ggtgaataat ccaaaagagt ggctgcaagt ggacttccag     1800

aagacaatga aagtcacagg agtaactact cagggagtaa aatctctgct taccagcatg     1860

tatgtgaagg agttcctcat ctccagcagt caagatggcc atcagtggac tctctttttt     1920

cagaatggca aagtaaaggt ttttcaggga aatcaagact ccttcacacc tgtggtgaac     1980

tctctagacc caccgttact gactcgctac cttcgaattc acccccagag ttgggtgcac     2040

cagattgccc tgaggatgga ggttctgggc tgcgaggcac aggacctcta ctgcggccgc     2100

cagctttcta gaacaaaaac tcatctcaga agaggatctg aatag                     2145


<210>  4
<211>  714
<212>  PRT
<213>  Homo sapiens


<220>
<221>  PEPTIDE
<222>  (1)..(714)

<400>  4

Glu Phe Thr Trp Pro Ser Arg Pro Ser Arg Ile Gly Thr Glu Ile Thr 
1               5                   10                  15      


Arg Thr Thr Leu Gln Ser Asp Gln Glu Glu Ile Asp Tyr Asp Asp Thr 
            20                  25                  30          


Ile Ser Val Glu Met Lys Lys Glu Asp Phe Asp Ile Tyr Asp Glu Asp 
        35                  40                  45              


Glu Asn Gln Ser Pro Arg Ser Phe Gln Lys Lys Thr Arg His Tyr Phe 
    50                  55                  60                  


Ile Ala Ala Val Glu Arg Leu Trp Asp Tyr Gly Met Ser Ser Ser Pro 
65                  70                  75                  80  


His Val Leu Arg Asn Arg Ala Gln Ser Gly Ser Val Pro Gln Phe Lys 
                85                  90                  95      


Lys Val Val Phe Gln Glu Phe Thr Asp Gly Ser Phe Thr Gln Pro Leu 
            100                 105                 110         


Tyr Arg Gly Glu Leu Asn Glu His Leu Gly Leu Leu Gly Pro Tyr Ile 
        115                 120                 125             


Arg Ala Glu Val Glu Asp Asn Ile Met Val Thr Phe Arg Asn Gln Ala 
    130                 135                 140                 


Ser Arg Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Glu Glu Asp 
145                 150                 155                 160 


Gln Arg Gln Gly Ala Glu Pro Arg Lys Asn Phe Val Lys Pro Asn Glu 
                165                 170                 175     


Thr Lys Thr Tyr Phe Trp Lys Val Gln His His Met Ala Pro Thr Lys 
            180                 185                 190         


Asp Glu Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu 
        195                 200                 205             


Glu Lys Asp Val His Ser Gly Leu Ile Gly Pro Leu Leu Val Cys His 
    210                 215                 220                 


Thr Asn Thr Leu Asn Pro Ala His Gly Arg Gln Val Thr Val Gln Glu 
225                 230                 235                 240 


Phe Ala Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe 
                245                 250                 255     


Thr Glu Asn Met Glu Arg Asn Cys Arg Ala Pro Cys Asn Ile Gln Met 
            260                 265                 270         


Glu Asp Pro Thr Phe Lys Glu Asn Tyr Arg Phe His Ala Ile Asn Gly 
        275                 280                 285             


Tyr Ile Met Asp Thr Leu Pro Gly Leu Val Met Ala Gln Asp Gln Arg 
    290                 295                 300                 


Ile Arg Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His Ser 
305                 310                 315                 320 


Ile His Phe Ser Gly His Val Phe Thr Val Arg Lys Lys Glu Glu Tyr 
                325                 330                 335     


Lys Met Ala Leu Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val Glu 
            340                 345                 350         


Met Leu Pro Ser Lys Ala Gly Ile Trp Arg Val Glu Cys Leu Ile Gly 
        355                 360                 365             


Glu His Leu His Ala Gly Met Ser Thr Leu Phe Leu Val Tyr Ser Asn 
    370                 375                 380                 


Lys Cys Gln Thr Pro Leu Gly Met Ala Ser Gly His Ile Arg Asp Phe 
385                 390                 395                 400 


Gln Ile Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala 
                405                 410                 415     


Arg Leu His Tyr Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Glu Pro 
            420                 425                 430         


Phe Ser Trp Ile Lys Val Asp Leu Leu Ala Pro Met Ile Ile His Gly 
        435                 440                 445             


Ile Lys Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser 
    450                 455                 460                 


Gln Phe Ile Ile Met Tyr Ser Leu Asp Gly Lys Lys Trp Gln Thr Tyr 
465                 470                 475                 480 


Arg Gly Asn Ser Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val Asp 
                485                 490                 495     


Ser Ser Gly Ile Lys His Asn Ile Phe Asn Pro Pro Ile Ile Ala Arg 
            500                 505                 510         


Tyr Ile Arg Leu His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu Arg 
        515                 520                 525             


Met Glu Leu Met Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu Gly 
    530                 535                 540                 


Met Glu Ser Lys Ala Ile Ser Asp Ala Gln Ile Thr Ala Ser Ser Tyr 
545                 550                 555                 560 


Phe Thr Asn Met Phe Ala Thr Trp Ser Pro Ser Lys Ala Arg Leu His 
                565                 570                 575     


Leu Gln Gly Arg Ser Asn Ala Trp Arg Pro Gln Val Asn Asn Pro Lys 
            580                 585                 590         


Glu Trp Leu Gln Val Asp Phe Gln Lys Thr Met Lys Val Thr Gly Val 
        595                 600                 605             


Thr Thr Gln Gly Val Lys Ser Leu Leu Thr Ser Met Tyr Val Lys Glu 
    610                 615                 620                 


Phe Leu Ile Ser Ser Ser Gln Asp Gly His Gln Trp Thr Leu Phe Phe 
625                 630                 635                 640 


Gln Asn Gly Lys Val Lys Val Phe Gln Gly Asn Gln Asp Ser Phe Thr 
                645                 650                 655     


Pro Val Val Asn Ser Leu Asp Pro Pro Leu Leu Thr Arg Tyr Leu Arg 
            660                 665                 670         


Ile His Pro Gln Ser Trp Val His Gln Ile Ala Leu Arg Met Glu Val 
        675                 680                 685             


Leu Gly Cys Glu Ala Gln Asp Leu Tyr Cys Gly Arg Gln Leu Ser Arg 
    690                 695                 700                 


Thr Lys Thr His Leu Arg Arg Gly Ser Glu 
705                 710                 


