                         SEQUENCE LISTING

<110>  Laboratorios del Dr. Esteve, S.A.
       FundaciO Privada Institut de Recerca de Ia SIDA-Caixa
 
<120>  Methods for Identifying HIV Neutralizing Antibodies

<130>  P8746PC00

<150>  EP1238242
<151>  2012-09-06

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD4 L sense primer

<400>  1
caccatgaac cggggagtcc cttttag                                           27


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD4L AS NheI primer

<400>  2
tattagctag caccacgatg tctattttg                                         29


<210>  3
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MPER-mIgG1-S primer

<400>  3
gaatagagct ggtgggctag ctgtgcccag ggattgtggt                             40


<210>  4
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mIgG1-AS primer

<400>  4
ttattctcga gtcatttacc aggagagtgg g                                      31


<210>  5
<211>  609
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fragment of the huCD4mIgG1 fusion protein nucleotide sequence

<400>  5
atgaaccggg gagtcccttt taggcacttg cttctggtgc tgcaactggc gctcctccca       60

gcagccactc agggaaagaa agtggtgctg ggcaaaaaag gggatacagt ggaactgacc      120

tgtacagctt cccagaagaa gagcatacaa ttccactgga aaaactccaa ccagataaag      180

attctgggaa atcagggctc cttcttaact aaaggtccat ccaagctgaa tgatcgcgct      240

gactcaagaa gaagcctttg ggaccaagga aacttccccc tgatcatcaa gaatcttaag      300

atagaagact cagatactta catctgtgaa gtggaggacc agaaggagga ggtgcaattg      360

ctagtgttcg gattgactgc caactctgac acccacctgc ttcaggggca gagcctgacc      420

ctgaccttgg agagcccccc tggtagtagc ccctcagtgc aatgtaggag tccaaggggt      480

aaaaacatac agggggggaa gaccctctcc gtgtctcagc tggagctcca ggatagtggc      540

acctggacat gcactgtctt gcagaaccag aagaaggtgg agttcaaaat agacatcgtg      600

gtgctagct                                                              609


<210>  6
<211>  690
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fragment of the huCD4mIgG1 fusion protein nucleotide sequence

<400>  6
gtgcccaggg attgtggttg taagccttgc atatgtacag tcccagaagt atcatctgtc       60

ttcatcttcc ccccaaagcc caaggatgtg ctcaccatta ctctgactcc taaggtcacg      120

tgtgttgtgg tagacatcag caaggatgat cccgaggtcc agttcagctg gtttgtagat      180

gatgtggagg tgcacacagc tcagacgcaa ccccgggagg agcagttcaa cagcactttc      240

cgctcagtca gtgaacttcc catcatgcac caggactggc tcaatggcaa ggagttcaaa      300

tgcagggtca acagtgcagc tttccctgcc cccatcgaga aaaccatctc caaaaccaaa      360

ggcagaccga aggctccaca ggtgtacacc attccacctc ccaaggagca gatggccaag      420

gataaagtca gtctgacctg catgataaca gacttcttcc ctgaagacat tactgtggag      480

tggcagtgga atgggcagcc agcggagaac tacaagaaca ctcagcccat catggacaca      540

gatggctctt acttcgtcta cagcaagctc aatgtgcaga agagcaactg ggaggcagga      600

aatactttca cctgctctgt gttacatgag ggcctgcaca accaccatac tgagaagagc      660

ctctcccact ctcctggtaa atgactcgag                                       690


<210>  7
<211>  430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD4-Fc fusion protein

<400>  7

Met Asn Arg Gly Val Pro Phe Arg His Leu Leu Leu Val Leu Gln Leu 
1               5                   10                  15      


Ala Leu Leu Pro Ala Ala Thr Gln Gly Lys Lys Val Val Leu Gly Lys 
            20                  25                  30          


Lys Gly Asp Thr Val Glu Leu Thr Cys Thr Ala Ser Gln Lys Lys Ser 
        35                  40                  45              


Ile Gln Phe His Trp Lys Asn Ser Asn Gln Ile Lys Ile Leu Gly Asn 
    50                  55                  60                  


Gln Gly Ser Phe Leu Thr Lys Gly Pro Ser Lys Leu Asn Asp Arg Ala 
65                  70                  75                  80  


Asp Ser Arg Arg Ser Leu Trp Asp Gln Gly Asn Phe Pro Leu Ile Ile 
                85                  90                  95      


Lys Asn Leu Lys Ile Glu Asp Ser Asp Thr Tyr Ile Cys Glu Val Glu 
            100                 105                 110         


Asp Gln Lys Glu Glu Val Gln Leu Leu Val Phe Gly Leu Thr Ala Asn 
        115                 120                 125             


Ser Asp Thr His Leu Leu Gln Gly Gln Ser Leu Thr Leu Thr Leu Glu 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ser Pro Ser Val Gln Cys Arg Ser Pro Arg Gly 
145                 150                 155                 160 


Lys Asn Ile Gln Gly Gly Lys Thr Leu Ser Val Ser Gln Leu Glu Leu 
                165                 170                 175     


Gln Asp Ser Gly Thr Trp Thr Cys Thr Val Leu Gln Asn Gln Lys Lys 
            180                 185                 190         


Val Glu Phe Lys Ile Asp Ile Val Val Leu Ala Val Pro Arg Asp Cys 
        195                 200                 205             


Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe 
    210                 215                 220                 


Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro 
225                 230                 235                 240 


Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val 
                245                 250                 255     


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr 
            260                 265                 270         


Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu 
        275                 280                 285             


Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 
    290                 295                 300                 


Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser 
305                 310                 315                 320 


Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro 
                325                 330                 335     


Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile 
            340                 345                 350         


Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly 
        355                 360                 365             


Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp 
    370                 375                 380                 


Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp 
385                 390                 395                 400 


Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His 
                405                 410                 415     


Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
            420                 425                 430 


<210>  8
<211>  203
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD4 fragment

<400>  8

Met Asn Arg Gly Val Pro Phe Arg His Leu Leu Leu Val Leu Gln Leu 
1               5                   10                  15      


Ala Leu Leu Pro Ala Ala Thr Gln Gly Lys Lys Val Val Leu Gly Lys 
            20                  25                  30          


Lys Gly Asp Thr Val Glu Leu Thr Cys Thr Ala Ser Gln Lys Lys Ser 
        35                  40                  45              


Ile Gln Phe His Trp Lys Asn Ser Asn Gln Ile Lys Ile Leu Gly Asn 
    50                  55                  60                  


Gln Gly Ser Phe Leu Thr Lys Gly Pro Ser Lys Leu Asn Asp Arg Ala 
65                  70                  75                  80  


Asp Ser Arg Arg Ser Leu Trp Asp Gln Gly Asn Phe Pro Leu Ile Ile 
                85                  90                  95      


Lys Asn Leu Lys Ile Glu Asp Ser Asp Thr Tyr Ile Cys Glu Val Glu 
            100                 105                 110         


Asp Gln Lys Glu Glu Val Gln Leu Leu Val Phe Gly Leu Thr Ala Asn 
        115                 120                 125             


Ser Asp Thr His Leu Leu Gln Gly Gln Ser Leu Thr Leu Thr Leu Glu 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ser Pro Ser Val Gln Cys Arg Ser Pro Arg Gly 
145                 150                 155                 160 


Lys Asn Ile Gln Gly Gly Lys Thr Leu Ser Val Ser Gln Leu Glu Leu 
                165                 170                 175     


Gln Asp Ser Gly Thr Trp Thr Cys Thr Val Leu Gln Asn Gln Lys Lys 
            180                 185                 190         


Val Glu Phe Lys Ile Asp Ile Val Val Leu Ala 
        195                 200             


<210>  9
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fc fragment

<400>  9

Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu 
1               5                   10                  15      


Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr 
            20                  25                  30          


Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile Ser Lys 
        35                  40                  45              


Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val 
    50                  55                  60                  


His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe 
65                  70                  75                  80  


Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val 
        115                 120                 125             


Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser 
    130                 135                 140                 


Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu 
145                 150                 155                 160 


Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro 
                165                 170                 175     


Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val 
            180                 185                 190         


Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu 
        195                 200                 205             


His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser His Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  10
<211>  1293
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence encoding CD4-Fc fusion protein

<400>  10
atgaaccggg gagtcccttt taggcacttg cttctggtgc tgcaactggc gctcctccca       60

gcagccactc agggaaagaa agtggtgctg ggcaaaaaag gggatacagt ggaactgacc      120

tgtacagctt cccagaagaa gagcatacaa ttccactgga aaaactccaa ccagataaag      180

attctgggaa atcagggctc cttcttaact aaaggtccat ccaagctgaa tgatcgcgct      240

gactcaagaa gaagcctttg ggaccaagga aacttccccc tgatcatcaa gaatcttaag      300

atagaagact cagatactta catctgtgaa gtggaggacc agaaggagga ggtgcaattg      360

ctagtgttcg gattgactgc caactctgac acccacctgc ttcaggggca gagcctgacc      420

ctgaccttgg agagcccccc tggtagtagc ccctcagtgc aatgtaggag tccaaggggt      480

aaaaacatac agggggggaa gaccctctcc gtgtctcagc tggagctcca ggatagtggc      540

acctggacat gcactgtctt gcagaaccag aagaaggtgg agttcaaaat agacatcgtg      600

gtgctagctg tgcccaggga ttgtggttgt aagccttgca tatgtacagt cccagaagta      660

tcatctgtct tcatcttccc cccaaagccc aaggatgtgc tcaccattac tctgactcct      720

aaggtcacgt gtgttgtggt agacatcagc aaggatgatc ccgaggtcca gttcagctgg      780

tttgtagatg atgtggaggt gcacacagct cagacgcaac cccgggagga gcagttcaac      840

agcactttcc gctcagtcag tgaacttccc atcatgcacc aggactggct caatggcaag      900

gagttcaaat gcagggtcaa cagtgcagct ttccctgccc ccatcgagaa aaccatctcc      960

aaaaccaaag gcagaccgaa ggctccacag gtgtacacca ttccacctcc caaggagcag     1020

atggccaagg ataaagtcag tctgacctgc atgataacag acttcttccc tgaagacatt     1080

actgtggagt ggcagtggaa tgggcagcca gcggagaact acaagaacac tcagcccatc     1140

atggacacag atggctctta cttcgtctac agcaagctca atgtgcagaa gagcaactgg     1200

gaggcaggaa atactttcac ctgctctgtg ttacatgagg gcctgcacaa ccaccatact     1260

gagaagagcc tctcccactc tcctggtaaa tga                                  1293


