                         SEQUENCE LISTING

<110>  EveliQure Biotechnologies GmbH
 
<120>  A novel live attenuated Shigella vaccine

<130>  EQ001P

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  bacterial antigen


<220>
<221>  PEPTIDE
<222>  (12)..(12)
<223>  X can be N, K or R wherein Sequence NSSNYCCELCCNPACTGCY is 
       excluded

<220>
<221>  PEPTIDE
<222>  (13)..(13)
<223>  X can be P, G, L or F, wherein Sequence NSSNYCCELCCNPACTGCY is 
       excluded

<400>  1

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Xaa Xaa Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  2
<211>  19
<212>  PRT
<213>  Escherichia Sp.

<400>  2

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  3
<211>  63
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  3
cgcacgggct ggcgctcggc tgctgcatcg tcgatggtgt tccgtgtagg ctggagctgc       60

ttc                                                                     63


<210>  4
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  4
tatcagtctt cacatcggca ttttgcgccc gctgccgtaa catatgaata tcctccttag       60

ttcctattaa                                                              70


<210>  5
<211>  61
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  5
gaatagtaat atttacgctg tcattgtgac atataatccc ggtgtaggct ggagctgctt       60

c                                                                       61


<210>  6
<211>  68
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  6
gcattataac gaccgccccc agtaattcct cttattccca tatgaatatc ctccttagtt       60

cctaatcc                                                                68


<210>  7
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  7
gagccgtgat ggctggaaac ac                                                22


<210>  8
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  8
agcgcaatcg cggttttgtt ca                                                22


<210>  9
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  9
gggttactgg gtgccgcaat atcc                                              24


<210>  10
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  Oligonucleotide

<400>  10
cctcaatcca gcattcgcca ttatacg                                           27


<210>  11
<211>  796
<212>  DNA
<213>  artificial

<220>
<223>  Construct

<400>  11
gctgccgtgg ttcaagtcgc gactaataaa aataatcagg ttgccatgat tcaatgtaca       60

cctttctcac attcgtctcc ggcatgaaaa cgatgcactc tttctttatc gctttcacta      120

cacattttat cctcgcatgg atgttttata aaaaacatga ttgacatcat gttgcatata      180

ggttaaacaa aacaagtggc gttatctttt tccggattgt cttcttgtat gatatataag      240

ttttcctcga tgaataaagt aaaatgttat gttttattta cggcgttact atcctctcta      300

tgtgcatacg gagctcccca gtctattaca gaactatgtt cggaatatcg caacacacaa      360

atatatacga taaatgacaa gatactatca tatacggaat cgatggcagg caaaagagaa      420

atggttatca ttacatttaa gagcggcgca acatttcagg tcgaagtccc gggcagtcaa      480

catatagact cccaaaaaaa agccattgaa aggatgaagg acacattaag aatcacatat      540

ctgaccgaga ccaaaattga taaattatgt gtatggaata ataaaacccc caattcaatt      600

gcggcaatca gtatggaaaa cgggccgggg cccaattctt ctaactactg ctgtgaactt      660

tgttgtaatt ttgcctgtac aggatgttac tagtttgctt taaaagcatg tctaatgcta      720

ggaacctata taacaactac tgtacttata ctaatgagcc ttatgctgca tttgaaaagg      780

cggtagagga tgcaat                                                      796


<210>  12
<211>  147
<212>  PRT
<213>  artificial

<220>
<223>  Construct

<400>  12

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Leu Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn Gly Pro Gly Pro 
        115                 120                 125             


Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Phe Ala Cys Thr 
    130                 135                 140                 


Gly Cys Tyr 
145         


<210>  13
<211>  799
<212>  DNA
<213>  artificial

<220>
<223>  Construct

<400>  13
gctgccgtgg ttcaagtcgc gactaataaa aataatcagg ttgccatgat tcaatgtaca       60

cctttctcac attcgtctcc ggcatgaaaa cgatgcactc tttctttatc gctttcacta      120

cacattttat cctcgcatgg atgttttata aaaaacatga ttgacatcat gttgcatata      180

ggttaaacaa aacaagtggc gttatctttt tccggattgt cttcttgtat gatatataag      240

ttttcctcga tgaataaagt aaaatgttat gttttattta cggcgttact atcctctcta      300

tgtgcatacg gagctcccca gtctattaca gaactatgtt cggaatatcg caacacacaa      360

atatatacga taaatgacaa gatactatca tatacggaat cgatggcagg caaaagagaa      420

atggttatca ttacatttaa gagcggcgca acatttcagg tcgaagtccc gggcagtcaa      480

catatagact cccaaaaaaa agccattgaa aggatgaagg acacattaag aatcacatat      540

ctgaccgaga ccaaaattga taaattatgt gtatggaata ataaaacccc caattcaatt      600

gcggcaatca gtatggaaaa cggcggaggt ggctccaatt cttctaacta ctgctgtgaa      660

ctttgttgta atggcgcctg tacaggatgt tactagtttg ctttaaaagc atgtctaatg      720

ctaggaacct atataacaac tactgtactt atactaatga gccttatgct gcatttgaaa      780

aggcggtaga ggatgcaat                                                   799


<210>  14
<211>  148
<212>  PRT
<213>  artificial

<220>
<223>  Construct

<400>  14

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Leu Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn Gly Gly Gly Gly 
        115                 120                 125             


Ser Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Gly Ala Cys 
    130                 135                 140                 


Thr Gly Cys Tyr 
145             


<210>  15
<211>  799
<212>  DNA
<213>  artificial

<220>
<223>  Construct

<400>  15
gctgccgtgg ttcaagtcgc gactaataaa aataatcagg ttgccatgat tcaatgtaca       60

cctttctcac attcgtctcc ggcatgaaaa cgatgcactc tttctttatc gctttcacta      120

cacattttat cctcgcatgg atgttttata aaaaacatga ttgacatcat gttgcatata      180

ggttaaacaa aacaagtggc gttatctttt tccggattgt cttcttgtat gatatataag      240

ttttcctcga tgaataaagt aaaatgttat gttttattta cggcgttact atcctctcta      300

tgtgcatacg gagctcccca gtctattaca gaactatgtt cggaatatcg caacacacaa      360

atatatacga taaatgacaa gatactatca tatacggaat cgatggcagg caaaagagaa      420

atggttatca ttacatttaa gagcggcgca acatttcagg tcgaagtccc gggcagtcaa      480

catatagact cccaaaaaaa agccattgaa aggatgaagg acacattaag aatcacatat      540

ctgaccgaga ccaaaattga taaattatgt gtatggaata ataaaacccc caattcaatt      600

gcggcaatca gtatggaaaa cggcggaggt ggctccaatt cttctaacta ctgctgtgaa      660

ctttgttgtc gccctgcctg tacaggatgt tactagtttg ctttaaaagc atgtctaatg      720

ctaggaacct atataacaac tactgtactt atactaatga gccttatgct gcatttgaaa      780

aggcggtaga ggatgcaat                                                   799


<210>  16
<211>  148
<212>  PRT
<213>  artificial

<220>
<223>  Construct

<400>  16

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Leu Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn Gly Gly Gly Gly 
        115                 120                 125             


Ser Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Arg Pro Ala Cys 
    130                 135                 140                 


Thr Gly Cys Tyr 
145             


<210>  17
<211>  799
<212>  DNA
<213>  artificial

<220>
<223>  Construct

<400>  17
gctgccgtgg ttcaagtcgc gactaataaa aataatcagg ttgccatgat tcaatgtaca       60

cctttctcac attcgtctcc ggcatgaaaa cgatgcactc tttctttatc gctttcacta      120

cacattttat cctcgcatgg atgttttata aaaaacatga ttgacatcat gttgcatata      180

ggttaaacaa aacaagtggc gttatctttt tccggattgt cttcttgtat gatatataag      240

ttttcctcga tgaataaagt aaaatgttat gttttattta cggcgttact atcctctcta      300

tgtgcatacg gagctcccca gtctattaca gaactatgtt cggaatatcg caacacacaa      360

atatatacga taaatgacaa gatactatca tatacggaat cgatggcagg caaaagagaa      420

atggttatca ttacatttaa gagcggcgca acatttcagg tcgaagtccc gggcagtcaa      480

catatagact cccaaaaaaa agccattgaa aggatgaagg acacattaag aatcacatat      540

ctgaccgaga ccaaaattga taaattatgt gtatggaata ataaaacccc caattcaatt      600

gcggcaatca gtatggaaaa cggcggaggt ggctccaatt cttctaacta ctgctgtgaa      660

ctttgttgta aacctgcctg tacaggatgt tactagtttg ctttaaaagc atgtctaatg      720

ctaggaacct atataacaac tactgtactt atactaatga gccttatgct gcatttgaaa      780

aggcggtaga ggatgcaat                                                   799


<210>  18
<211>  148
<212>  PRT
<213>  artificial

<220>
<223>  Construct

<400>  18

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Leu Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn Gly Gly Gly Gly 
        115                 120                 125             


Ser Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Lys Pro Ala Cys 
    130                 135                 140                 


Thr Gly Cys Tyr 
145             


<210>  19
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Primer

<400>  19
gtaagcacca caaccactgg                                                   20


<210>  20
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  Primer

<400>  20
ccagcaatct gactggctgt cg                                                22


<210>  21
<211>  77
<212>  DNA
<213>  artificial

<220>
<223>  Construct

<400>  21
gccaaaatat tggcttccac tgagcttgga gacaatacta tccaagccat atgaatatcc       60

tccttagttc ctaatcc                                                      77


<210>  22
<211>  65
<212>  DNA
<213>  artificial

<220>
<223>  Construct

<400>  22
gtattaattg atttgtcgct tgggatgctt ctttagatac ttggggtgta ggctggagct       60

gcttc                                                                   65


<210>  23
<211>  2854
<212>  DNA
<213>  artificial

<220>
<223>  Gene

<400>  23
atgcataatg taagcaccac aaccactggt tttcctcttg ccaaaatatt ggcttccact       60

gagcttggag acaatactat ccaagctgca aatgatgcag ctaacaaatt attttctctt      120

acaattgctg atcttactgc taaccaaaat attaatacaa ctaatgcaca ctcaacttca      180

aatatattaa tccctgaact taaagcacca aagtcattaa atgcaagttc ccaactaacg      240

cttttaattg gaaaccttat tcaaatactc ggtgaaaaat ctttaactgc attaacaaat      300

aaaattactg cttggaagtc ccagcaacag gcaagacagc aaaaaaacct agaattctcc      360

gataaaatta acactcttct atctgaaact gaaggactaa ccagagacta tgaaaaacaa      420

attaataaac taaaaaacgc agattctaaa ataaaagacc tagaaaataa aattaaccaa      480

attcaaacaa gattatccga actcgaccca gagtcaccag aaaagaaaaa attaagccgg      540

gaagaaatac aactcactat caaaaaagac gcagcagtta aagacaggac attgattgag      600

cagaaaaccc tgtcaattca tagcaaactt acagataaat caatgcaact cgaaaaagaa      660

atagactctt tttctgcatt ttcaaacaca gcatctgctg aacagctatc aacccagcag      720

aaatcattaa ccggacttgc cagtgttact caattgatgg caacctttat tcaactagtt      780

ggaaaaaata atgaagaatc tttaaaaaat gatctggctc tattccagtc tctccaagaa      840

tcaagaaaaa ctgaaatgga gagaaaatct gatgagtatg ctgctgaagt acgtaaagca      900

gaagaactca acagagtaat gggttgtgtt gggaaaatac ttggggcact tttaactatc      960

gttagtgttg ttgcagcagc tttttctgga ggagcctctc tagcactggc agctgttggt     1020

ttagctctta tggttacgga tgctatagta caagcagcga ccggcaattc cttcatggaa     1080

caagccctga atccgatcat gaaagcagtc attgaaccct taatcaaact cctttcagat     1140

gcatttacaa aaatgctcga aggcttgggc gtcgactcga aaaaagccaa aatgattggc     1200

tctattctgg gggcaatcgc aggcgctctt gtcctagttg cagcagtcgt tctcgtagcc     1260

actgttggta aacaggcagc agcaaaactt gcagaaaata ttggcaaaat aataggtaaa     1320

accctcacag accttatacc aaagtttctc aagaattttt cttctcaact ggacgattta     1380

atcactaatg ctgttgccag attaaataaa tttcttggtg cagcgggtga tgaagtaata     1440

tccaaacaaa ttatttccac ccatttaaac caagcagttt tattaggaga aagtgttaac     1500

tctgccacac aagcgggagg aagtgtcgct tctgctgttt tccagaacag cgcgtcgaca     1560

aatctagcag acctgacatt atcgaaatat caagttgaac aactgtcaaa atatatcagt     1620

gaagcaatag aaaaattcgg ccaattgcag gaagtaattg cagatctatt agcctcaatg     1680

tccaactctc aggctaatag aactgatgtt gcaaaagcaa ttttgcaaca aactactgct     1740

tgatacaaat aaggagaatg ttatggaaat tcaaaacaca aaaccaaccc agattttata     1800

tacagatata tccacaaaac aaactcaaag ttcttccgaa acacaaaaat cacaaaatta     1860

tcagcagatt gcagcgcata ttccacttaa tgtcggtaaa aatcccgtat taacaaccac     1920

attaaatgat gatcaacttt taaagttatc agagcaggtt cagcatgatt cagaaatcat     1980

tgctcgcctt actgacaaaa agatgaaaga tcgttcagag atgagtcaca cccttactcc     2040

agagaacact ctggatattt ccagtctttc ttctaatgct gtttctttaa ttattagtgt     2100

agccgttcta ctttctgctc tccgcactgc agaaactaaa ttgggctctc aattgtcatt     2160

gattgcgttc gatgctacaa aatcagctgc agagaacatt gttcggcaag gcctggcagc     2220

cctatcatca agcattactg gagcagtcac acaagtaggt ataacgggta tcggtgccaa     2280

aaaaacgcat tcagggatta gcgaccaaaa aggagcctta agaaagaacc ttgccactgc     2340

tcaatctctt gaaaaagagc ttgcaggttc taaattaggg ttaaataaac aaatagatac     2400

aaatatcacc tcaccacaaa ctaactctag cacaaaattt ttaggtaaaa ataaactggc     2460

gccagataat atatccctgt caactgaaca taaaacttct cttagttctc ccgatatttc     2520

tttgcaggat aaaattgaca cccagagaag aacttacgag ctcaataccc tttctgcgca     2580

gcaaaaacaa aacattggcc gtgcaacaat ggaaacatca gccgttgctg gtaatatatc     2640

cacatcagga gggcgttatg catctgctct tgaagaagaa gaacaactaa tcagtcaggc     2700

cagcagtaaa caagcagagg aagcatccca agtatctaaa gaagcatccc aagcgacaaa     2760

tcaattaata caaaaattat tgaatataat tgacagcatc aaccaatcaa agaattcgac     2820

agccagtcag attgctggta acattcgagc ttaa                                 2854


<210>  24
<211>  938
<212>  DNA
<213>  artificial

<220>
<223>  Gene

<400>  24
aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat gaaaaatgcg       60

gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa aaaggaaaat      120

gaagacgaaa acaagcaaag acattcggcg cgagttggct ataatacttg gcacttgttt      180

gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg ggtaaagatc      240

tgccggaaga catctacgtt gttattgaga tcccggctaa cgcagatccg atcaaatacg      300

aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc gcgatgttct      360

atccgtgcaa ctacggttac atcaaccaca ccctgtctct ggacggtgac ccggttgacg      420

tactggtccc gactccgtac ccgctgcagc ctggttctgt gatccgttgc cgtccggttg      480

ccgttctgaa aatgaccgac gaagccggtg aagatgcaaa actggttgcg gttccgcaca      540

gcaagctgag caaagaatac gatcacatta aagacgtaaa cgatctgcct gaactgctga      600

aagcgcaaat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc aagtgggtga      660

aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgct tccttcgagc      720

gcgcaaagaa taaataagtt cttctagcgc aataaccctg aacgccgggc ttcggttagt      780

aagggttttt ttatgcccgc gataaataaa ctctctattc caccatcatt attctcagcg      840

gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct gttcatagcc      900

acttgctgga agttagccga cctcactcat actcaccg                              938


<210>  25
<211>  90
<212>  DNA
<213>  artificial

<220>
<223>  Primer

<400>  25
ttattattgg tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt       60

gcgttacatc gtgtaggctg gagctgcttc                                        90


<210>  26
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  Primer

<400>  26
tccatatctg caatcgcata aaaaactctg ctggcgttca caaatgtgca ggggtaaaac       60

gggggcacgc catatgaata tcctccttag ttcctaatcc                            100


<210>  27
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  Primer

<400>  27
catagggttg tcctcgtcgg gg                                                22


<210>  28
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  Primer

<400>  28
gttttatgcg atgtatctcg cg                                                22


<210>  29
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Linker

<400>  29

Gly Gly Gly Gly Ser 
1               5   


