P2012,0829 WO E / Isoprene				September 7, 2012

                         SEQUENCE LISTING

<110>  Algenol Biofuels Inc.
 
<120>  GENETICALLY ENHANCED CYANOBACTERIA FOR THE PRODUCTION OF ISOPRENE

<130>  E2012,0583

<160>  13    

<170>  PatentIn version 3.3

<210>  1
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  generalized sequence of promoter psaA*


<220>
<221>  misc_feature
<222>  (10)..(22)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(44)
<223>  n is a, c, g, or t

<400>  1
gtcttgactn nnnnnnnnnn nntgntataa tnnnnnnnnn nnnnaaggag gcaatcatat     60

g                                                                     61


<210>  2
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  psaA* promoter

<400>  2
gtcttgacta ggggggggga ggtggtataa tctactagtg aattaaggag gcaatcatat     60

g                                                                     61


<210>  3
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  fusion of the -35 sequence and the Pribnow-box of the native psaA
       promoter with the ribosomal binding site of the isiA promoter

<400>  3
gtcttgccta ggggggggga ggccgtatta tcttctagtg aattaaggag gcaattctgt     60

g                                                                     61


<210>  4
<211>  294
<212>  DNA
<213>  Synechocystis PCC6803

<400>  4
aatttttgta acaaagttca actttgtctt gacttttgta agtctttgca aaatctagga     60

gctagaactg gtcagggctg gggcaatttt taattattgt tacgcaggtc ttgcctaggg    120

ggggggaggc cgtattatct tctagtgatg tttgctgaaa acgcctatct gtgcaaggtt    180

taacatcgtt attatgaagc gaaaactaat tccctttttt acgcttcctc tattacacta    240

ttctgcatag gaaaccctta atagttcatt gtcgagcgag gagaaccctg catg          294


<210>  5
<211>  60
<212>  DNA
<213>  Synechocystis PCC6803

<400>  5
tctcggcact tattgccata atttattatt tgtcgtctca attaaggagg caattctgtg     60


<210>  6
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  exchange of four nucleotides from hybrid promoter between PsaA 
       and PisiA

<400>  6
gtcttgacta ggggggggga ggtggtataa tcttctagtg aattaaggag gcaattctgt     60

g                                                                     61


<210>  7
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  introduction of rerstriction sites into PpsaA*

<400>  7
gtcgacgtct tgactagggg gggggaggtg gtataatcta ctagtgaatt aaggaggcaa     60

tcatatg                                                               67


<210>  8
<211>  1695
<212>  DNA
<213>  Artificial

<220>
<223>  codon optimized gene of isoprene synthase from Pueraria Montana

<400>  8
atgtgtgcta ccagctctca atttacccaa attaccgaac ataatagtcg gcgttccgct     60

aattaccagc ccaacctctg gaattttgaa tttctgcaat ctctggaaaa tgaccttaag    120

gtggaaaaat tagaagagaa ggccaccaag ttagaggagg aggtacggtg catgatcaac    180

cgggtagaca cccaaccctt aagcttatta gaattgatcg acgatgtcca gcgtttaggt    240

ttgacctaca agtttgagaa ggacattatc aaagcccttg agaatattgt tttgctggat    300

gagaataaga aaaataaaag tgacctccat gctactgctc tcagcttccg tttacttcgg    360

caacatggct ttgaggtttc ccaagatgtg tttgagcggt ttaaggacaa ggagggtggt    420

ttcagtggtg aacttaaagg tgatgtgcaa gggttgctga gtttatatga agcctcctat    480

cttggctttg agggtgaaaa tctcttggag gaggcccgga ccttttccat tacccatctc    540

aagaacaact taaaagaagg tattaacacc aaagtggccg aacaagttag tcatgccctg    600

gaacttccct atcatcaacg gttgcatcgg ttagaagccc ggtggttcct tgacaaatat    660

gaacccaagg aaccccacca tcagttatta ctggagcttg ccaagttaga tttcaatatg    720

gtgcaaacct tgcaccagaa agaactgcaa gacctgtccc ggtggtggac ggagatgggg    780

ttagccagca agttagactt tgtccgggat cggttaatgg aagtgtattt ttgggcgttg    840

ggtatggccc ctgatcctca attcggtgaa tgtcgtaaag ctgtcactaa aatgtttggt    900

ttggtcacca tcattgatga tgtatatgac gtttatggta ctttggatga gttacaactc    960

ttcactgatg ctgttgagcg gtgggacgtg aatgccatta acacccttcc cgactacatg   1020

aagttgtgct tcttagccct ttataacacc gtcaatgaca cgtcttatag catccttaaa   1080

gaaaaaggtc acaacaacct ttcctatttg accaaatctt ggcgtgagtt atgcaaagcc   1140

ttccttcaag aagccaaatg gtccaacaac aaaatcattc ccgcctttag caagtacctg   1200

gaaaatgcct ccgtgtcctc ctccggtgtg gctttgcttg ctccttccta cttctccgtg   1260

tgccaacaac aagaagatat ctccgaccat gctcttcgtt ctttaactga ttttcatggc   1320

cttgtgcgct cctcctgcgt cattttccgg ctctgcaatg atttggctac ctccgcggct   1380

gagttagagc ggggtgagac gaccaattcc attatttctt atatgcacga gaatgacggc   1440

acttctgaag agcaagcccg tgaggagttg cggaaattga ttgatgccga gtggaagaag   1500

atgaaccggg agcgggtttc cgattctacc ttactcccca aagcttttat ggaaattgct   1560

gttaacatgg ctcgggtttc ccattgcacc taccaatatg gtgacggtct tggtcggccc   1620

gactacgcca ccgagaatcg gatcaagttg ttacttattg acccctttcc catcaatcaa   1680

ttaatgtacg tgtaa                                                    1695


<210>  9
<211>  564
<212>  PRT
<213>  Pueraria Montana 

<400>  9

Met Cys Ala Thr Ser Ser Gln Phe Thr Gln Ile Thr Glu His Asn Ser 
1               5                   10                  15      


Arg Arg Ser Ala Asn Tyr Gln Pro Asn Leu Trp Asn Phe Glu Phe Leu 
            20                  25                  30          


Gln Ser Leu Glu Asn Asp Leu Lys Val Glu Lys Leu Glu Glu Lys Ala 
        35                  40                  45              


Thr Lys Leu Glu Glu Glu Val Arg Cys Met Ile Asn Arg Val Asp Thr 
    50                  55                  60                  


Gln Pro Leu Ser Leu Leu Glu Leu Ile Asp Asp Val Gln Arg Leu Gly 
65                  70                  75                  80  


Leu Thr Tyr Lys Phe Glu Lys Asp Ile Ile Lys Ala Leu Glu Asn Ile 
                85                  90                  95      


Val Leu Leu Asp Glu Asn Lys Lys Asn Lys Ser Asp Leu His Ala Thr 
            100                 105                 110         


Ala Leu Ser Phe Arg Leu Leu Arg Gln His Gly Phe Glu Val Ser Gln 
        115                 120                 125             


Asp Val Phe Glu Arg Phe Lys Asp Lys Glu Gly Gly Phe Ser Gly Glu 
    130                 135                 140                 


Leu Lys Gly Asp Val Gln Gly Leu Leu Ser Leu Tyr Glu Ala Ser Tyr 
145                 150                 155                 160 


Leu Gly Phe Glu Gly Glu Asn Leu Leu Glu Glu Ala Arg Thr Phe Ser 
                165                 170                 175     


Ile Thr His Leu Lys Asn Asn Leu Lys Glu Gly Ile Asn Thr Lys Val 
            180                 185                 190         


Ala Glu Gln Val Ser His Ala Leu Glu Leu Pro Tyr His Gln Arg Leu 
        195                 200                 205             


His Arg Leu Glu Ala Arg Trp Phe Leu Asp Lys Tyr Glu Pro Lys Glu 
    210                 215                 220                 


Pro His His Gln Leu Leu Leu Glu Leu Ala Lys Leu Asp Phe Asn Met 
225                 230                 235                 240 


Val Gln Thr Leu His Gln Lys Glu Leu Gln Asp Leu Ser Arg Trp Trp 
                245                 250                 255     


Thr Glu Met Gly Leu Ala Ser Lys Leu Asp Phe Val Arg Asp Arg Leu 
            260                 265                 270         


Met Glu Val Tyr Phe Trp Ala Leu Gly Met Ala Pro Asp Pro Gln Phe 
        275                 280                 285             


Gly Glu Cys Arg Lys Ala Val Thr Lys Met Phe Gly Leu Val Thr Ile 
    290                 295                 300                 


Ile Asp Asp Val Tyr Asp Val Tyr Gly Thr Leu Asp Glu Leu Gln Leu 
305                 310                 315                 320 


Phe Thr Asp Ala Val Glu Arg Trp Asp Val Asn Ala Ile Asn Thr Leu 
                325                 330                 335     


Pro Asp Tyr Met Lys Leu Cys Phe Leu Ala Leu Tyr Asn Thr Val Asn 
            340                 345                 350         


Asp Thr Ser Tyr Ser Ile Leu Lys Glu Lys Gly His Asn Asn Leu Ser 
        355                 360                 365             


Tyr Leu Thr Lys Ser Trp Arg Glu Leu Cys Lys Ala Phe Leu Gln Glu 
    370                 375                 380                 


Ala Lys Trp Ser Asn Asn Lys Ile Ile Pro Ala Phe Ser Lys Tyr Leu 
385                 390                 395                 400 


Glu Asn Ala Ser Val Ser Ser Ser Gly Val Ala Leu Leu Ala Pro Ser 
                405                 410                 415     


Tyr Phe Ser Val Cys Gln Gln Gln Glu Asp Ile Ser Asp His Ala Leu 
            420                 425                 430         


Arg Ser Leu Thr Asp Phe His Gly Leu Val Arg Ser Ser Cys Val Ile 
        435                 440                 445             


Phe Arg Leu Cys Asn Asp Leu Ala Thr Ser Ala Ala Glu Leu Glu Arg 
    450                 455                 460                 


Gly Glu Thr Thr Asn Ser Ile Ile Ser Tyr Met His Glu Asn Asp Gly 
465                 470                 475                 480 


Thr Ser Glu Glu Gln Ala Arg Glu Glu Leu Arg Lys Leu Ile Asp Ala 
                485                 490                 495     


Glu Trp Lys Lys Met Asn Arg Glu Arg Val Ser Asp Ser Thr Leu Leu 
            500                 505                 510         


Pro Lys Ala Phe Met Glu Ile Ala Val Asn Met Ala Arg Val Ser His 
        515                 520                 525             


Cys Thr Tyr Gln Tyr Gly Asp Gly Leu Gly Arg Pro Asp Tyr Ala Thr 
    530                 535                 540                 


Glu Asn Arg Ile Lys Leu Leu Leu Ile Asp Pro Phe Pro Ile Asn Gln 
545                 550                 555                 560 


Leu Met Tyr Val 
                


<210>  10
<211>  11542
<212>  DNA
<213>  Artificial

<220>
<223>  plasmid pVZ325-PrbcL-IspSoop

<400>  10
tcgacatcag gaattgtaat tagaaagtcc aaaaattgta atttaaaaaa cagtcaatgg     60

agagcattgc cataagtaaa ggcatcccct gcgtgataag attaccttca gaaaacagat    120

agttgctggg ttatcgcaga tttttctcgc aaccaaataa ctgtaaataa taactgtctc    180

tggggcgacg gtaggcttta tattgccaaa tttcgcccgt gggagaaagc taggctattc    240

aatgtttatg gaggactgac catatgtgtg ctaccagctc tcaatttacc caaattaccg    300

aacataatag tcggcgttcc gctaattacc agcccaacct ctggaatttt gaatttctgc    360

aatctctgga aaatgacctt aaggtggaaa aattagaaga gaaggccacc aagttagagg    420

aggaggtacg gtgcatgatc aaccgggtag acacccaacc cttaagctta ttagaattga    480

tcgacgatgt ccagcgttta ggtttgacct acaagtttga gaaggacatt atcaaagccc    540

ttgagaatat tgttttgctg gatgagaata agaaaaataa aagtgacctc catgctactg    600

ctctcagctt ccgtttactt cggcaacatg gctttgaggt ttcccaagat gtgtttgagc    660

ggtttaagga caaggagggt ggtttcagtg gtgaacttaa aggtgatgtg caagggttgc    720

tgagtttata tgaagcctcc tatcttggct ttgagggtga aaatctcttg gaggaggccc    780

ggaccttttc cattacccat ctcaagaaca acttaaaaga aggtattaac accaaagtgg    840

ccgaacaagt tagtcatgcc ctggaacttc cctatcatca acggttgcat cggttagaag    900

cccggtggtt ccttgacaaa tatgaaccca aggaacccca ccatcagtta ttactggagc    960

ttgccaagtt agatttcaat atggtgcaaa ccttgcacca gaaagaactg caagacctgt   1020

cccggtggtg gacggagatg gggttagcca gcaagttaga ctttgtccgg gatcggttaa   1080

tggaagtgta tttttgggcg ttgggtatgg cccctgatcc tcaattcggt gaatgtcgta   1140

aagctgtcac taaaatgttt ggtttggtca ccatcattga tgatgtatat gacgtttatg   1200

gtactttgga tgagttacaa ctcttcactg atgctgttga gcggtgggac gtgaatgcca   1260

ttaacaccct tcccgactac atgaagttgt gcttcttagc cctttataac accgtcaatg   1320

acacgtctta tagcatcctt aaagaaaaag gtcacaacaa cctttcctat ttgaccaaat   1380

cttggcgtga gttatgcaaa gccttccttc aagaagccaa atggtccaac aacaaaatca   1440

ttcccgcctt tagcaagtac ctggaaaatg cctccgtgtc ctcctccggt gtggctttgc   1500

ttgctccttc ctacttctcc gtgtgccaac aacaagaaga tatctccgac catgctcttc   1560

gttctttaac tgattttcat ggccttgtgc gctcctcctg cgtcattttc cggctctgca   1620

atgatttggc tacctccgcg gctgagttag agcggggtga gacgaccaat tccattattt   1680

cttatatgca cgagaatgac ggcacttctg aagagcaagc ccgtgaggag ttgcggaaat   1740

tgattgatgc cgagtggaag aagatgaacc gggagcgggt ttccgattct accttactcc   1800

ccaaagcttt tatggaaatt gctgttaaca tggctcgggt ttcccattgc acctaccaat   1860

atggtgacgg tcttggtcgg cccgactacg ccaccgagaa tcggatcaag ttgttactta   1920

ttgacccctt tcccatcaat caattaatgt acgtgtaata acgctcggtt gccgccgggc   1980

gttttttatt cctgcaggag cagaagagca tacatctgga agcaaagcca ggaaagcggc   2040

ctatggagct gtgcggcagc gctcagtagg caatttttca aaatattgtt aagccttttc   2100

tgagcatggt atttttcatg gtattaccaa ttagcaggaa aataagccat tgaatataaa   2160

agataaaaat gtcttgttta caatagagtg gggggggtca gcctgccgcc ttgggccggg   2220

tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca gcccagcgcg accagctccg   2280

gcaacgcctc gcgcacccgc tggcggcgct tgcgcatggt cgaaccactg gcctctgacg   2340

gccagacata gccgcacaag gtatctatgg aagccttgcc ggttttgccg gggtcgatcc   2400

agccacacag ccgctggtgc agcaggcggg cggtttcgct gtccagcgcc cgcacctcgt   2460

ccatgctgat gcgcacatgc tggccgccac ccatgacggc ctgcgcgatc aaggggttca   2520

gggccacgta caggcgcccg tccgcctcgt cgctggcgta ctccgacagc agccgaaacc   2580

cctgccgctt gcggccattc tgggcgatga tggatacctt ccaaaggcgc tcgatgcagt   2640

cctgtatgtg cttgagcgcc ccaccactat cgacctctgc cccgatttcc tttgccagcg   2700

cccgatagct acctttgacc acatggcatt cagcggtgac ggcctcccac ttgggttcca   2760

ggaacagccg gagctgccgt ccgccttcgg tcttgggttc cgggccaagc actaggccat   2820

taggcccagc catggccacc agcccttgca ggatgcgcag atcatcagcg cccagcggct   2880

ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc atacgtcacg tccagcttgc   2940

tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc gggggccaga cagtgcgccg   3000

ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg cttcaccacg gggcaccccc   3060

ttgctcttgc gctgcctctc cagcacggcg ggcttgagca ccccgccgtc atgccgcctg   3120

aaccaccgat cagcgaacgg tgcgccatag ttggccttgc tcacaccgaa gcggacgaag   3180

aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct cggcgctggt catgctcgac   3240

aggtaggact gccagcggat gttatcgacc agtaccgagc tgccccggct ggcctgctgc   3300

tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat ggtgcaggaa cacgatagag   3360

cacccggtat cggcggcgat ggcctccatg cgaccgatga cctgggccat ggggccgctg   3420

gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca gcaccatcag gcggcggccc   3480

tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca tgatgttggg caggctgccg   3540

atcagcggct ggatcagcag gccgtcagcc acggcttgcc gttcctcggc gctgaggtgc   3600

gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg ggtcttcggc gggcaggtag   3660

atcaccgggc cggtgggcag ttcgcccacc tccagcagat ccggcccgcc tgcaatctgt   3720

gcggccagtt gcagggccag catggattta ccggcaccac cgggcgacac cagcgccccg   3780

accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg gtggcggcgc tgctgcgaac   3840

gcctccagaa tattgatagg cttatgggta gccattgatt gcctcctttg caggcagttg   3900

gtggttaggc gctggcgggg tcactacccc cgccctgcgc cgctctgagt tcttccaggc   3960

actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa cttgcgctga cgcatccctt   4020

tggccttcat gcgctcggca tatcgcgctt ggcgtacagc gtcagggctg gccagcaggt   4080

cgccggtctg cttgtccttt tggtctttca tatcagtcac cgagaaactt gccggggccg   4140

aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt caaggttaag gctggccata   4200

tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg tataaccaaa gccaccgggc   4260

aaccaatagc ccttgtcact tttgatcagg tagaccgacc ctgaagcgct tttttcgtat   4320

tccataaaac ccccttctgt gcgtgagtac tcatagtata acaggcgtga gtaccaacgc   4380

aagcactaca tgctgaaatc tggcccgccc ctgtccatgc ctcgctggcg gggtgccggt   4440

gcccgtgcca gctcggcccg cgcaagctgg acgctgggca gacccatgac cttgctgacg   4500

gtgcgctcga tgtaatccgc ttcgtggccg ggcttgcgct ctgccagcgc tgggctggcc   4560

tcggccatgg ccttgccgat ttcctcggca ctgcggcccc ggctggccag cttctgcgcg   4620

gcgataaagt cgcacttgct gaggtcatca ccgaagcgct tgaccagccc ggccatctcg   4680

ctgcggtact cgtccagcgc cgtgcgccgg tggcggctaa gctgccgctc gggcagttcg   4740

aggctggcca gcctgcgggc cttctcctgc tgccgctggg cctgctcgat ctgctggcca   4800

gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc ccttggattc acgcagcagc   4860

acccacggct gataaccggc gcgggtggtg tgcttgtcct tgcggttggt gaagcccgcc   4920

aagcggccat agtggcggct gtcggcgctg gccgggtcgg cgtcgtactc gctggccagc   4980

gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt cggccacctt gacccatgcc   5040

tgatagttct tcgggctggt ttccactacc agggcaggct cccggccctc ggctttcatg   5100

tcatccaggt caaactcgct gaggtcgtcc accagcacca gaccatgccg ctcctgctcg   5160

gcgggcctga tatacacgtc attgccctgg gcattcatcc gcttgagcca tggcgtgttc   5220

tggagcactt cggcggctga ccattcccgg ttcatcatct ggccggtggt ggcgtccctg   5280

acgccgatat cgaagcgctc acagcccatg gccttgagct gtcggcctat ggcctgcaaa   5340

gtcctgtcgt tcttcatcgg gccaccaagc gcagccagat cgagccgtcc tcggttgtca   5400

gtggcgtcag gtcgagcaag agcaacgatg cgatcagcag caccaccgta ggcatcatgg   5460

aagccagcat cacggttagc catagcttcc agtgccaccc ccgcgacgcg ctccgggcgc   5520

tctgcgcggc gctgctcacc tcggcggcta cctcccgcaa ctctttggcc agctccaccc   5580

atgccgcccc tgtctggcgc tgggctttca gccactccgc cgcctgcgcc tcgctggcct   5640

gctgggtctg gctcatgacc tgccgggctt cgtcggccag tgtcgccatg ctctgggcca   5700

gcggttcgat ctgctccgct aactcgttga tgcctctgga tttcttcact ctgtcgattg   5760

cgttcatggt ctattgcctc ccggtattcc tgtaagtcga tgatctgggc gttggcggtg   5820

tcgatgttca gggccacgtc tgcccggtcg gtgcggatgc cccggccttc catctccacc   5880

acgttcggcc ccaggtgaac accgggcagg cgctcgatgc cctgcgcctc aagtgttctg   5940

tggtcaatgc gggcgtcgtg gccagcccgc tctaatgccc ggttggcatg gtcggcccat   6000

gcctcgcggg tctgctcaag ccatgccttg ggcttgagcg cttcggtctt ctgtgccccg   6060

cccttctccg gggtcttgcc gttgtaccgc ttgaaccact gagcggcggg ccgctcgatg   6120

ccgtcattga tccgctcgga gatcatcagg tggcagtgcg ggttctcgcc gccaccggca   6180

tggatggcca gcgtatacgg caggcgctcg gcaccggtca ggtgctgggc gaactcggac   6240

gccagcgcct tctgctggtc gagggtcagc tcgaccggca gggcaaattc gacctccttg   6300

aacagccgcc cattggcgcg ttcatacagg tcggcagcat cccagtagtc ggcgggccgc   6360

tcgacgaact ccggcatgtg cccggattcg gcgtgcaaga cttcatccat gtcgcgggca   6420

tacttgcctt cgcgctggat gtagtcggcc ttggccctgg ccgattggcc gcccgacctg   6480

ctgccggttt tcgccgtaag gtgataaatc gccatgctgc ctcgctgttg cttttgcttt   6540

tcggctccat gcaatggccc tcggagagcg caccgcccga agggtggccg ttaggccagt   6600

ttctcgaaga gaaaccggta agtgcgccct cccctacaaa gtagggtcgg gattgccgcc   6660

gctgtgcctc catgatagcc tacgagacag cacattaaca atggggtgtc aagatggtta   6720

aggggagcaa caaggcggcg gatcggctgg ccaagctcga agaacaacga gcgcgaatca   6780

atgccgaaat tcagcgggtg cgggcaaggg aacagcagca agagcgcaag aacgaaacaa   6840

ggcgcaaggt gctggtgggg gccatgattt tggccaaggt gaacagcagc gagtggccgg   6900

aggatcggct catggcggca atggatgcgt accttgaacg cgaccacgac cgcgccttgt   6960

tcggtctgcc gccacgccag aaggatgagc cgggctgaat gatcgaccga gacaggccct   7020

gcggggctgc acacgcgccc ccacccttcg ggtaggggga aaggccgcta aagcggctaa   7080

aagcgctcca gcgtatttct gcggggtttg gtgtggggtt tagcgggctt tgcccgcctt   7140

tccccctgcc gcgcagcggt ggggcggtgt gtagcctagc gcagcgaata gaccagctat   7200

ccggcctctg gccgggcata ttgggcaagg gcagcagcgc cccacaaggg cgctgataac   7260

cgcgcctagt ggattattct tagataatca tggatggatt tttccaacac cccgccagcc   7320

cccgcccctg ctgggtttgc aggtttgggg gcgtgacagt tattgcaggg gttcgtgaca   7380

gttattgcag gggggcgtga cagttattgc aggggttcgt gacagttagt acgggagtga   7440

cgggcactgg ctggcaatgt ctagcaacgg caggcatttc ggctgagggt aaaagaactt   7500

tccgctaagc gatagactgt atgtaaacac agtattgcaa ggacgcggaa catgcctcat   7560

gtggcggcca ggacggccag ccgggatcgg gatactggtc gttaccagag ccaccgaccc   7620

gagcaaaccc ttctctatca gatcgttgac gagtattacc cggcattcgc tgcgcttatg   7680

gcagagcagg gaaaggaatt gccgggctat gtgcaacggg aatttgaaga atttctccaa   7740

tgcgggcggc tggagcatgg ctttctacgg gttcgctgcg agtcttgcca cgccgagcac   7800

ctggtcgctt tcagctgtaa tccgggcagc gcaacggaac attcatcagt gtaaaaatgg   7860

aatcaataaa gccctgcgca gcgcgcaggg tcagcctgaa tacgcgttta atgaccagca   7920

cagtcgtgat ggcaaggtca gaatagcgct gaggtctgcc tcgtgaagaa ggtgttgctg   7980

actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga   8040

tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac   8100

ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta   8160

ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata   8220

tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga   8280

gccatattca acgggaaacg tcttgctcga gaccgagctc gaattggccg cggcgttgtg   8340

acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg   8400

tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat   8460

agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa   8520

gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt   8580

gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacctgg   8640

gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga   8700

tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt   8760

aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga   8820

cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg   8880

ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc   8940

cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata   9000

gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg   9060

ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt   9120

ttacgaaccg aacaggctta tgtcaattcg agcatcgatt gtatgggaag cccgatgcgc   9180

cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg   9240

tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta   9300

ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat   9360

tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc   9420

ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg   9480

ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc   9540

gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac   9600

cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga   9660

aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg   9720

ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa   9780

aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt   9840

ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac   9900

gggacggcgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca   9960

tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc  10020

cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc  10080

gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctattc  10140

tgaccttgcc atcacgactg tgctggtcat taaacgcgta ttcaggctga ccctgcgcgc  10200

tgcgcagggc tttattgatt ccatttttac actgatgaat gttccgttgc gctgcccgga  10260

ttacagatcc tctagaagaa cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa  10320

accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt  10380

gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt  10440

tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg  10500

accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt  10560

tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga  10620

tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag  10680

ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt  10740

ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca  10800

gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg  10860

cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct  10920

ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt  10980

ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt  11040

cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa  11100

gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt  11160

cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc  11220

gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca  11280

gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg  11340

gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat  11400

cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc  11460

accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg  11520

cggcttaact caagctctag ag                                           11542


<210>  11
<211>  13418
<212>  DNA
<213>  Artificial

<220>
<223>  plasmid pVZ325-Ptac/lacI-IspSoop

<400>  11
tcgaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc     60

gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc    120

atctgtgcgg tatttcacac cgcataaatt ccgacaccat cgaatggtgc aaaacctttc    180

gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag    240

taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg    300

tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg    360

agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga    420

ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta    480

aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg    540

tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca    600

ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc    660

cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg    720

aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc    780

tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat    840

atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt    900

ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg    960

ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg   1020

ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc   1080

cgccgtcaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct   1140

tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg   1200

tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg   1260

attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac   1320

gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg   1380

gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac   1440

catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt   1500

tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga   1560

ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc   1620

ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc   1680

cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac   1740

caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg   1800

ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat   1860

tatttttgat ggcgttggaa ttacgttatc gactgcacgg tgcaccaatg cttctggcgt   1920

caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt   1980

gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc   2040

tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa   2100

ttgtgagcgg ataacaattt cacacaggaa acagtacata tgtgtgctac cagctctcaa   2160

tttacccaaa ttaccgaaca taatagtcgg cgttccgcta attaccagcc caacctctgg   2220

aattttgaat ttctgcaatc tctggaaaat gaccttaagg tggaaaaatt agaagagaag   2280

gccaccaagt tagaggagga ggtacggtgc atgatcaacc gggtagacac ccaaccctta   2340

agcttattag aattgatcga cgatgtccag cgtttaggtt tgacctacaa gtttgagaag   2400

gacattatca aagcccttga gaatattgtt ttgctggatg agaataagaa aaataaaagt   2460

gacctccatg ctactgctct cagcttccgt ttacttcggc aacatggctt tgaggtttcc   2520

caagatgtgt ttgagcggtt taaggacaag gagggtggtt tcagtggtga acttaaaggt   2580

gatgtgcaag ggttgctgag tttatatgaa gcctcctatc ttggctttga gggtgaaaat   2640

ctcttggagg aggcccggac cttttccatt acccatctca agaacaactt aaaagaaggt   2700

attaacacca aagtggccga acaagttagt catgccctgg aacttcccta tcatcaacgg   2760

ttgcatcggt tagaagcccg gtggttcctt gacaaatatg aacccaagga accccaccat   2820

cagttattac tggagcttgc caagttagat ttcaatatgg tgcaaacctt gcaccagaaa   2880

gaactgcaag acctgtcccg gtggtggacg gagatggggt tagccagcaa gttagacttt   2940

gtccgggatc ggttaatgga agtgtatttt tgggcgttgg gtatggcccc tgatcctcaa   3000

ttcggtgaat gtcgtaaagc tgtcactaaa atgtttggtt tggtcaccat cattgatgat   3060

gtatatgacg tttatggtac tttggatgag ttacaactct tcactgatgc tgttgagcgg   3120

tgggacgtga atgccattaa cacccttccc gactacatga agttgtgctt cttagccctt   3180

tataacaccg tcaatgacac gtcttatagc atccttaaag aaaaaggtca caacaacctt   3240

tcctatttga ccaaatcttg gcgtgagtta tgcaaagcct tccttcaaga agccaaatgg   3300

tccaacaaca aaatcattcc cgcctttagc aagtacctgg aaaatgcctc cgtgtcctcc   3360

tccggtgtgg ctttgcttgc tccttcctac ttctccgtgt gccaacaaca agaagatatc   3420

tccgaccatg ctcttcgttc tttaactgat tttcatggcc ttgtgcgctc ctcctgcgtc   3480

attttccggc tctgcaatga tttggctacc tccgcggctg agttagagcg gggtgagacg   3540

accaattcca ttatttctta tatgcacgag aatgacggca cttctgaaga gcaagcccgt   3600

gaggagttgc ggaaattgat tgatgccgag tggaagaaga tgaaccggga gcgggtttcc   3660

gattctacct tactccccaa agcttttatg gaaattgctg ttaacatggc tcgggtttcc   3720

cattgcacct accaatatgg tgacggtctt ggtcggcccg actacgccac cgagaatcgg   3780

atcaagttgt tacttattga cccctttccc atcaatcaat taatgtacgt gtaataacgc   3840

tcggttgccg ccgggcgttt tttattcctg caggagcaga agagcataca tctggaagca   3900

aagccaggaa agcggcctat ggagctgtgc ggcagcgctc agtaggcaat ttttcaaaat   3960

attgttaagc cttttctgag catggtattt ttcatggtat taccaattag caggaaaata   4020

agccattgaa tataaaagat aaaaatgtct tgtttacaat agagtggggg gggtcagcct   4080

gccgccttgg gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc   4140

agcgcgacca gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa   4200

ccactggcct ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt   4260

ttgccggggt cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc   4320

agcgcccgca cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc   4380

gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc   4440

gacagcagcc gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa   4500

aggcgctcga tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg   4560

atttcctttg ccagcgcccg atagctacct ttgaccacat ggcattcagc ggtgacggcc   4620

tcccacttgg gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg   4680

ccaagcacta ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca   4740

tcagcgccca gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac   4800

gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg   4860

gccagacagt gcgccgggtc gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc   4920

accacggggc acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc   4980

gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac   5040

accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc   5100

gctggtcatg ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc   5160

ccggctggcc tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg   5220

caggaacacg atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg   5280

ggccatgggg ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac   5340

catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat   5400

gttgggcagg ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc   5460

ctcggcgctg aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc   5520

ttcggcgggc aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg   5580

cccgcctgca atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg   5640

cgacaccagc gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg   5700

cggcgctgct gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct   5760

cctttgcagg cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct   5820

ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg   5880

cgctgacgca tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca   5940

gggctggcca gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag   6000

aaacttgccg gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag   6060

gttaaggctg gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata   6120

accaaagcca ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga   6180

agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag   6240

gcgtgagtac caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg   6300

ctggcggggt gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc   6360

catgaccttg ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc   6420

cagcgctggg ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct   6480

ggccagcttc tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac   6540

cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg   6600

ccgctcgggc agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg   6660

ctcgatctgc tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt   6720

ggattcacgc agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg   6780

gttggtgaag cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc   6840

gtactcgctg gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc   6900

caccttgacc catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg   6960

gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc   7020

atgccgctcc tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt   7080

gagccatggc gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc   7140

ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg   7200

gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca ccaagcgcag ccagatcgag   7260

ccgtcctcgg ttgtcagtgg cgtcaggtcg agcaagagca acgatgcgat cagcagcacc   7320

accgtaggca tcatggaagc cagcatcacg gttagccata gcttccagtg ccacccccgc   7380

gacgcgctcc gggcgctctg cgcggcgctg ctcacctcgg cggctacctc ccgcaactct   7440

ttggccagct ccacccatgc cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc   7500

tgcgcctcgc tggcctgctg ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc   7560

gccatgctct gggccagcgg ttcgatctgc tccgctaact cgttgatgcc tctggatttc   7620

ttcactctgt cgattgcgtt catggtctat tgcctcccgg tattcctgta agtcgatgat   7680

ctgggcgttg gcggtgtcga tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg   7740

gccttccatc tccaccacgt tcggccccag gtgaacaccg ggcaggcgct cgatgccctg   7800

cgcctcaagt gttctgtggt caatgcgggc gtcgtggcca gcccgctcta atgcccggtt   7860

ggcatggtcg gcccatgcct cgcgggtctg ctcaagccat gccttgggct tgagcgcttc   7920

ggtcttctgt gccccgccct tctccggggt cttgccgttg taccgcttga accactgagc   7980

ggcgggccgc tcgatgccgt cattgatccg ctcggagatc atcaggtggc agtgcgggtt   8040

ctcgccgcca ccggcatgga tggccagcgt atacggcagg cgctcggcac cggtcaggtg   8100

ctgggcgaac tcggacgcca gcgccttctg ctggtcgagg gtcagctcga ccggcagggc   8160

aaattcgacc tccttgaaca gccgcccatt ggcgcgttca tacaggtcgg cagcatccca   8220

gtagtcggcg ggccgctcga cgaactccgg catgtgcccg gattcggcgt gcaagacttc   8280

atccatgtcg cgggcatact tgccttcgcg ctggatgtag tcggccttgg ccctggccga   8340

ttggccgccc gacctgctgc cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg   8400

ctgttgcttt tgcttttcgg ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg   8460

tggccgttag gccagtttct cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag   8520

ggtcgggatt gccgccgctg tgcctccatg atagcctacg agacagcaca ttaacaatgg   8580

ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc ggctggccaa gctcgaagaa   8640

caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg caagggaaca gcagcaagag   8700

cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca tgattttggc caaggtgaac   8760

agcagcgagt ggccggagga tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac   8820

cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg atgagccggg ctgaatgatc   8880

gaccgagaca ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg   8940

ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc   9000

gggctttgcc cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag   9060

cgaatagacc agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca   9120

caagggcgct gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc   9180

caacaccccg ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt   9240

gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca   9300

gttagtacgg gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct   9360

gagggtaaaa gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac   9420

gcggaacatg cctcatgtgg cggccaggac ggccagccgg gatcgggata ctggtcgtta   9480

ccagagccac cgacccgagc aaacccttct ctatcagatc gttgacgagt attacccggc   9540

attcgctgcg cttatggcag agcagggaaa ggaattgccg ggctatgtgc aacgggaatt   9600

tgaagaattt ctccaatgcg ggcggctgga gcatggcttt ctacgggttc gctgcgagtc   9660

ttgccacgcc gagcacctgg tcgctttcag ctgtaatccg ggcagcgcaa cggaacattc   9720

atcagtgtaa aaatggaatc aataaagccc tgcgcagcgc gcagggtcag cctgaatacg   9780

cgtttaatga ccagcacagt cgtgatggca aggtcagaat agcgctgagg tctgcctcgt   9840

gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg   9900

agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt   9960

tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca  10020

gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt  10080

gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat  10140

acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgagacc gagctcgaat  10200

tggccgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct  10260

tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt  10320

atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag  10380

atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca  10440

atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc  10500

ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg  10560

aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc  10620

ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca  10680

gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt  10740

gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt  10800

aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt  10860

ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac  10920

tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg  10980

ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aattcgagca tcgattgtat  11040

gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat  11100

gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc  11160

aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa  11220

acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg  11280

gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat  11340

cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt  11400

gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag  11460

cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt  11520

atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac  11580

cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag  11640

aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat  11700

ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc  11760

attacgctga cttgacggga cggcggcttt gttgaataaa tcgaactttt gctgagttga  11820

aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca  11880

aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct  11940

ggctggatga tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca  12000

gacctcagcg ctattctgac cttgccatca cgactgtgct ggtcattaaa cgcgtattca  12060

ggctgaccct gcgcgctgcg cagggcttta ttgattccat ttttacactg atgaatgttc  12120

cgttgcgctg cccggattac agatcctcta gaagaacagc aaggccgcca atgcctgacg  12180

atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc  12240

gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca  12300

gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa  12360

ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc  12420

ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg  12480

ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag  12540

tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca  12600

actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca  12660

tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt  12720

tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga  12780

aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt  12840

gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg  12900

gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc  12960

tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga  13020

actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct  13080

atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg  13140

catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc  13200

aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct  13260

tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta  13320

cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg  13380

acgccgcttc gcggcgcggc ttaactcaag ctctagag                          13418


<210>  12
<211>  11342
<212>  DNA
<213>  Artificial

<220>
<223>  plasmid pVZ325-PpsaA*-IspSoop

<400>  12
tcgacgtctt gactaggggg ggggaggtgg tataatctac tagtgaatta aggaggcaat     60

catatgtgtg ctaccagctc tcaatttacc caaattaccg aacataatag tcggcgttcc    120

gctaattacc agcccaacct ctggaatttt gaatttctgc aatctctgga aaatgacctt    180

aaggtggaaa aattagaaga gaaggccacc aagttagagg aggaggtacg gtgcatgatc    240

aaccgggtag acacccaacc cttaagctta ttagaattga tcgacgatgt ccagcgttta    300

ggtttgacct acaagtttga gaaggacatt atcaaagccc ttgagaatat tgttttgctg    360

gatgagaata agaaaaataa aagtgacctc catgctactg ctctcagctt ccgtttactt    420

cggcaacatg gctttgaggt ttcccaagat gtgtttgagc ggtttaagga caaggagggt    480

ggtttcagtg gtgaacttaa aggtgatgtg caagggttgc tgagtttata tgaagcctcc    540

tatcttggct ttgagggtga aaatctcttg gaggaggccc ggaccttttc cattacccat    600

ctcaagaaca acttaaaaga aggtattaac accaaagtgg ccgaacaagt tagtcatgcc    660

ctggaacttc cctatcatca acggttgcat cggttagaag cccggtggtt ccttgacaaa    720

tatgaaccca aggaacccca ccatcagtta ttactggagc ttgccaagtt agatttcaat    780

atggtgcaaa ccttgcacca gaaagaactg caagacctgt cccggtggtg gacggagatg    840

gggttagcca gcaagttaga ctttgtccgg gatcggttaa tggaagtgta tttttgggcg    900

ttgggtatgg cccctgatcc tcaattcggt gaatgtcgta aagctgtcac taaaatgttt    960

ggtttggtca ccatcattga tgatgtatat gacgtttatg gtactttgga tgagttacaa   1020

ctcttcactg atgctgttga gcggtgggac gtgaatgcca ttaacaccct tcccgactac   1080

atgaagttgt gcttcttagc cctttataac accgtcaatg acacgtctta tagcatcctt   1140

aaagaaaaag gtcacaacaa cctttcctat ttgaccaaat cttggcgtga gttatgcaaa   1200

gccttccttc aagaagccaa atggtccaac aacaaaatca ttcccgcctt tagcaagtac   1260

ctggaaaatg cctccgtgtc ctcctccggt gtggctttgc ttgctccttc ctacttctcc   1320

gtgtgccaac aacaagaaga tatctccgac catgctcttc gttctttaac tgattttcat   1380

ggccttgtgc gctcctcctg cgtcattttc cggctctgca atgatttggc tacctccgcg   1440

gctgagttag agcggggtga gacgaccaat tccattattt cttatatgca cgagaatgac   1500

ggcacttctg aagagcaagc ccgtgaggag ttgcggaaat tgattgatgc cgagtggaag   1560

aagatgaacc gggagcgggt ttccgattct accttactcc ccaaagcttt tatggaaatt   1620

gctgttaaca tggctcgggt ttcccattgc acctaccaat atggtgacgg tcttggtcgg   1680

cccgactacg ccaccgagaa tcggatcaag ttgttactta ttgacccctt tcccatcaat   1740

caattaatgt acgtgtaata acgctcggtt gccgccgggc gttttttatt cctgcaggag   1800

cagaagagca tacatctgga agcaaagcca ggaaagcggc ctatggagct gtgcggcagc   1860

gctcagtagg caatttttca aaatattgtt aagccttttc tgagcatggt atttttcatg   1920

gtattaccaa ttagcaggaa aataagccat tgaatataaa agataaaaat gtcttgttta   1980

caatagagtg gggggggtca gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc   2040

gcgaactcgg ttaccgtcca gcccagcgcg accagctccg gcaacgcctc gcgcacccgc   2100

tggcggcgct tgcgcatggt cgaaccactg gcctctgacg gccagacata gccgcacaag   2160

gtatctatgg aagccttgcc ggttttgccg gggtcgatcc agccacacag ccgctggtgc   2220

agcaggcggg cggtttcgct gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc   2280

tggccgccac ccatgacggc ctgcgcgatc aaggggttca gggccacgta caggcgcccg   2340

tccgcctcgt cgctggcgta ctccgacagc agccgaaacc cctgccgctt gcggccattc   2400

tgggcgatga tggatacctt ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc   2460

ccaccactat cgacctctgc cccgatttcc tttgccagcg cccgatagct acctttgacc   2520

acatggcatt cagcggtgac ggcctcccac ttgggttcca ggaacagccg gagctgccgt   2580

ccgccttcgg tcttgggttc cgggccaagc actaggccat taggcccagc catggccacc   2640

agcccttgca ggatgcgcag atcatcagcg cccagcggct ccgggccgct gaactcgatc   2700

cgcttgccgt cgccgtagtc atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc   2760

ttgagggcac ggaacaggcc gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg   2820

aggctgtgct tgttcttagg cttcaccacg gggcaccccc ttgctcttgc gctgcctctc   2880

cagcacggcg ggcttgagca ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg   2940

tgcgccatag ttggccttgc tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc   3000

cacaccccat tcctcggcct cggcgctggt catgctcgac aggtaggact gccagcggat   3060

gttatcgacc agtaccgagc tgccccggct ggcctgctgc tggtcgcctg cgcccatcat   3120

ggccgcgccc ttgctggcat ggtgcaggaa cacgatagag cacccggtat cggcggcgat   3180

ggcctccatg cgaccgatga cctgggccat ggggccgctg gcgttttctt cctcgatgtg   3240

gaaccggcgc agcgtgtcca gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc   3300

gtcgaaccac tccggggcca tgatgttggg caggctgccg atcagcggct ggatcagcag   3360

gccgtcagcc acggcttgcc gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg   3420

gtgatgaatg gcggtgggcg ggtcttcggc gggcaggtag atcaccgggc cggtgggcag   3480

ttcgcccacc tccagcagat ccggcccgcc tgcaatctgt gcggccagtt gcagggccag   3540

catggattta ccggcaccac cgggcgacac cagcgccccg accgtaccgg ccaccatgtt   3600

gggcaaaacg tagtccagcg gtggcggcgc tgctgcgaac gcctccagaa tattgatagg   3660

cttatgggta gccattgatt gcctcctttg caggcagttg gtggttaggc gctggcgggg   3720

tcactacccc cgccctgcgc cgctctgagt tcttccaggc actcgcgcag cgcctcgtat   3780

tcgtcgtcgg tcagccagaa cttgcgctga cgcatccctt tggccttcat gcgctcggca   3840

tatcgcgctt ggcgtacagc gtcagggctg gccagcaggt cgccggtctg cttgtccttt   3900

tggtctttca tatcagtcac cgagaaactt gccggggccg aaaggcttgt cttcgcggaa   3960

caaggacaag gtgcagccgt caaggttaag gctggccata tcagcgactg aaaagcggcc   4020

agcctcggcc ttgtttgacg tataaccaaa gccaccgggc aaccaatagc ccttgtcact   4080

tttgatcagg tagaccgacc ctgaagcgct tttttcgtat tccataaaac ccccttctgt   4140

gcgtgagtac tcatagtata acaggcgtga gtaccaacgc aagcactaca tgctgaaatc   4200

tggcccgccc ctgtccatgc ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg   4260

cgcaagctgg acgctgggca gacccatgac cttgctgacg gtgcgctcga tgtaatccgc   4320

ttcgtggccg ggcttgcgct ctgccagcgc tgggctggcc tcggccatgg ccttgccgat   4380

ttcctcggca ctgcggcccc ggctggccag cttctgcgcg gcgataaagt cgcacttgct   4440

gaggtcatca ccgaagcgct tgaccagccc ggccatctcg ctgcggtact cgtccagcgc   4500

cgtgcgccgg tggcggctaa gctgccgctc gggcagttcg aggctggcca gcctgcgggc   4560

cttctcctgc tgccgctggg cctgctcgat ctgctggcca gcctgctgca ccagcgccgg   4620

gccagcggtg gcggtcttgc ccttggattc acgcagcagc acccacggct gataaccggc   4680

gcgggtggtg tgcttgtcct tgcggttggt gaagcccgcc aagcggccat agtggcggct   4740

gtcggcgctg gccgggtcgg cgtcgtactc gctggccagc gtccgggcaa tctgcccccg   4800

aagttcaccg cctgcggcgt cggccacctt gacccatgcc tgatagttct tcgggctggt   4860

ttccactacc agggcaggct cccggccctc ggctttcatg tcatccaggt caaactcgct   4920

gaggtcgtcc accagcacca gaccatgccg ctcctgctcg gcgggcctga tatacacgtc   4980

attgccctgg gcattcatcc gcttgagcca tggcgtgttc tggagcactt cggcggctga   5040

ccattcccgg ttcatcatct ggccggtggt ggcgtccctg acgccgatat cgaagcgctc   5100

acagcccatg gccttgagct gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg   5160

gccaccaagc gcagccagat cgagccgtcc tcggttgtca gtggcgtcag gtcgagcaag   5220

agcaacgatg cgatcagcag caccaccgta ggcatcatgg aagccagcat cacggttagc   5280

catagcttcc agtgccaccc ccgcgacgcg ctccgggcgc tctgcgcggc gctgctcacc   5340

tcggcggcta cctcccgcaa ctctttggcc agctccaccc atgccgcccc tgtctggcgc   5400

tgggctttca gccactccgc cgcctgcgcc tcgctggcct gctgggtctg gctcatgacc   5460

tgccgggctt cgtcggccag tgtcgccatg ctctgggcca gcggttcgat ctgctccgct   5520

aactcgttga tgcctctgga tttcttcact ctgtcgattg cgttcatggt ctattgcctc   5580

ccggtattcc tgtaagtcga tgatctgggc gttggcggtg tcgatgttca gggccacgtc   5640

tgcccggtcg gtgcggatgc cccggccttc catctccacc acgttcggcc ccaggtgaac   5700

accgggcagg cgctcgatgc cctgcgcctc aagtgttctg tggtcaatgc gggcgtcgtg   5760

gccagcccgc tctaatgccc ggttggcatg gtcggcccat gcctcgcggg tctgctcaag   5820

ccatgccttg ggcttgagcg cttcggtctt ctgtgccccg cccttctccg gggtcttgcc   5880

gttgtaccgc ttgaaccact gagcggcggg ccgctcgatg ccgtcattga tccgctcgga   5940

gatcatcagg tggcagtgcg ggttctcgcc gccaccggca tggatggcca gcgtatacgg   6000

caggcgctcg gcaccggtca ggtgctgggc gaactcggac gccagcgcct tctgctggtc   6060

gagggtcagc tcgaccggca gggcaaattc gacctccttg aacagccgcc cattggcgcg   6120

ttcatacagg tcggcagcat cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg   6180

cccggattcg gcgtgcaaga cttcatccat gtcgcgggca tacttgcctt cgcgctggat   6240

gtagtcggcc ttggccctgg ccgattggcc gcccgacctg ctgccggttt tcgccgtaag   6300

gtgataaatc gccatgctgc ctcgctgttg cttttgcttt tcggctccat gcaatggccc   6360

tcggagagcg caccgcccga agggtggccg ttaggccagt ttctcgaaga gaaaccggta   6420

agtgcgccct cccctacaaa gtagggtcgg gattgccgcc gctgtgcctc catgatagcc   6480

tacgagacag cacattaaca atggggtgtc aagatggtta aggggagcaa caaggcggcg   6540

gatcggctgg ccaagctcga agaacaacga gcgcgaatca atgccgaaat tcagcgggtg   6600

cgggcaaggg aacagcagca agagcgcaag aacgaaacaa ggcgcaaggt gctggtgggg   6660

gccatgattt tggccaaggt gaacagcagc gagtggccgg aggatcggct catggcggca   6720

atggatgcgt accttgaacg cgaccacgac cgcgccttgt tcggtctgcc gccacgccag   6780

aaggatgagc cgggctgaat gatcgaccga gacaggccct gcggggctgc acacgcgccc   6840

ccacccttcg ggtaggggga aaggccgcta aagcggctaa aagcgctcca gcgtatttct   6900

gcggggtttg gtgtggggtt tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt   6960

ggggcggtgt gtagcctagc gcagcgaata gaccagctat ccggcctctg gccgggcata   7020

ttgggcaagg gcagcagcgc cccacaaggg cgctgataac cgcgcctagt ggattattct   7080

tagataatca tggatggatt tttccaacac cccgccagcc cccgcccctg ctgggtttgc   7140

aggtttgggg gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga   7200

cagttattgc aggggttcgt gacagttagt acgggagtga cgggcactgg ctggcaatgt   7260

ctagcaacgg caggcatttc ggctgagggt aaaagaactt tccgctaagc gatagactgt   7320

atgtaaacac agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag   7380

ccgggatcgg gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca   7440

gatcgttgac gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt   7500

gccgggctat gtgcaacggg aatttgaaga atttctccaa tgcgggcggc tggagcatgg   7560

ctttctacgg gttcgctgcg agtcttgcca cgccgagcac ctggtcgctt tcagctgtaa   7620

tccgggcagc gcaacggaac attcatcagt gtaaaaatgg aatcaataaa gccctgcgca   7680

gcgcgcaggg tcagcctgaa tacgcgttta atgaccagca cagtcgtgat ggcaaggtca   7740

gaatagcgct gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc   7800

gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg   7860

gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga   7920

tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg   7980

tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa   8040

ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg   8100

tcttgctcga gaccgagctc gaattggccg cggcgttgtg acaatttacc gaacaactcc   8160

gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata   8220

tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg   8280

tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg   8340

aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga   8400

tcatagatat agatctcact acgcggctgc tcaaacctgg gcagaacgta agccgcgaga   8460

gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc   8520

aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac   8580

tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta   8640

catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc   8700

tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc   8760

cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc   8820

ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct   8880

ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta   8940

tgtcaattcg agcatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat   9000

ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg   9060

gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta   9120

ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca   9180

ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt   9240

tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg   9300

aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa   9360

caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat   9420

ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat   9480

gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc   9540

ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct   9600

gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt   9660

aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa   9720

taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc   9780

gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca   9840

tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt   9900

cagcaacacc ttcttcacga ggcagacctc agcgctattc tgaccttgcc atcacgactg   9960

tgctggtcat taaacgcgta ttcaggctga ccctgcgcgc tgcgcagggc tttattgatt  10020

ccatttttac actgatgaat gttccgttgc gctgcccgga ttacagatcc tctagaagaa  10080

cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag  10140

ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg  10200

gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg  10260

caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa  10320

cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc  10380

tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag  10440

caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc  10500

ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct  10560

cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc  10620

acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg  10680

agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg  10740

cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa  10800

gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc  10860

agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc  10920

cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga  10980

ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg  11040

aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc  11100

gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat  11160

acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga  11220

tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata  11280

atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagctctag  11340

ag                                                                 11342


<210>  13
<211>  10330
<212>  DNA
<213>  Artificial

<220>
<223>  plasmid pVZ325-PpetJ-IspSoop

<400>  13
atagtccaaa agctgacttt cccctccatg ctctgggggg aattgctctg gcaactgatt     60

aatccactga gcaacagccc aagacacgca aacaaaaacc aacgtcttgg cgatcgccat    120

cggcaccatg aaaccatcgt aaaagctggg gaaagaataa aaaacagtgg ttcaggaatt    180

gcattgccat ggccacttca caaacctagc caattttagc ttgaccgcaa ctttgacaga    240

ttgtcttttg actttgcctg gaccgcctcc cataatacct tcgcgtcttg aagactttat    300

ccttgaaagg agaacatatg tgtgctacca gctctcaatt tacccaaatt accgaacata    360

atagtcggcg ttccgctaat taccagccca acctctggaa ttttgaattt ctgcaatctc    420

tggaaaatga ccttaaggtg gaaaaattag aagagaaggc caccaagtta gaggaggagg    480

tacggtgcat gatcaaccgg gtagacaccc aacccttaag cttattagaa ttgatcgacg    540

atgtccagcg tttaggtttg acctacaagt ttgagaagga cattatcaaa gcccttgaga    600

atattgtttt gctggatgag aataagaaaa ataaaagtga cctccatgct actgctctca    660

gcttccgttt acttcggcaa catggctttg aggtttccca agatgtgttt gagcggttta    720

aggacaagga gggtggtttc agtggtgaac ttaaaggtga tgtgcaaggg ttgctgagtt    780

tatatgaagc ctcctatctt ggctttgagg gtgaaaatct cttggaggag gcccggacct    840

tttccattac ccatctcaag aacaacttaa aagaaggtat taacaccaaa gtggccgaac    900

aagttagtca tgccctggaa cttccctatc atcaacggtt gcatcggtta gaagcccggt    960

ggttccttga caaatatgaa cccaaggaac cccaccatca gttattactg gagcttgcca   1020

agttagattt caatatggtg caaaccttgc accagaaaga actgcaagac ctgtcccggt   1080

ggtggacgga gatggggtta gccagcaagt tagactttgt ccgggatcgg ttaatggaag   1140

tgtatttttg ggcgttgggt atggcccctg atcctcaatt cggtgaatgt cgtaaagctg   1200

tcactaaaat gtttggtttg gtcaccatca ttgatgatgt atatgacgtt tatggtactt   1260

tggatgagtt acaactcttc actgatgctg ttgagcggtg ggacgtgaat gccattaaca   1320

cccttcccga ctacatgaag ttgtgcttct tagcccttta taacaccgtc aatgacacgt   1380

cttatagcat ccttaaagaa aaaggtcaca acaacctttc ctatttgacc aaatcttggc   1440

gtgagttatg caaagccttc cttcaagaag ccaaatggtc caacaacaaa atcattcccg   1500

cctttagcaa gtacctggaa aatgcctccg tgtcctcctc cggtgtggct ttgcttgctc   1560

cttcctactt ctccgtgtgc caacaacaag aagatatctc cgaccatgct cttcgttctt   1620

taactgattt tcatggcctt gtgcgctcct cctgcgtcat tttccggctc tgcaatgatt   1680

tggctacctc cgcggctgag ttagagcggg gtgagacgac caattccatt atttcttata   1740

tgcacgagaa tgacggcact tctgaagagc aagcccgtga ggagttgcgg aaattgattg   1800

atgccgagtg gaagaagatg aaccgggagc gggtttccga ttctacctta ctccccaaag   1860

cttttatgga aattgctgtt aacatggctc gggtttccca ttgcacctac caatatggtg   1920

acggtcttgg tcggcccgac tacgccaccg agaatcggat caagttgtta cttattgacc   1980

cctttcccat caatcaatta atgtacgtgt aataacgctc ggttgccgcc gggcgttttt   2040

tattcctgca ggagcagaag agcatacatc tggaagcaaa gccaggaaag cggcctatgg   2100

agctgtgcgg cagcgctcag taggcaattt ttcaaaatat tgttaagcct tttctgagca   2160

tggtattttt catggtatta ccaattagca ggaaaataag ccattgaata taaaagataa   2220

aaatgtcttg tttacaatag agtggggggg gtcagcctgc cgccttgggc cgggtgatgt   2280

cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg   2340

cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga   2400

catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac   2460

acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc   2520

tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca   2580

cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc   2640

gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta   2700

tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat   2760

agctaccttt gaccacatgg cattcagcgg tgacggcctc ccacttgggt tccaggaaca   2820

gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc   2880

cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc   2940

cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct   3000

tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt   3060

gccggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc   3120

ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac   3180

cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg   3240

cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag   3300

gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg   3360

cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg   3420

gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt   3480

tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg   3540

gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc   3600

ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca   3660

agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag gtagatcacc   3720

gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc   3780

agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta   3840

ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc   3900

agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt   3960

aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc   4020

gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct   4080

tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg   4140

tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc   4200

ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg   4260

actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa   4320

tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata   4380

aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac   4440

tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt   4500

gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc   4560

tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc   4620

atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata   4680

aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg   4740

tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg   4800

gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc   4860

tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac   4920

ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg   4980

ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg   5040

gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag   5100

ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc   5160

aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc   5220

ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc   5280

acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg   5340

atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg   5400

tcgttcttca tcgggccacc aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg   5460

tcaggtcgag caagagcaac gatgcgatca gcagcaccac cgtaggcatc atggaagcca   5520

gcatcacggt tagccatagc ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg   5580

cggcgctgct cacctcggcg gctacctccc gcaactcttt ggccagctcc acccatgccg   5640

cccctgtctg gcgctgggct ttcagccact ccgccgcctg cgcctcgctg gcctgctggg   5700

tctggctcat gacctgccgg gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt   5760

cgatctgctc cgctaactcg ttgatgcctc tggatttctt cactctgtcg attgcgttca   5820

tggtctattg cctcccggta ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg   5880

ttcagggcca cgtctgcccg gtcggtgcgg atgccccggc cttccatctc caccacgttc   5940

ggccccaggt gaacaccggg caggcgctcg atgccctgcg cctcaagtgt tctgtggtca   6000

atgcgggcgt cgtggccagc ccgctctaat gcccggttgg catggtcggc ccatgcctcg   6060

cgggtctgct caagccatgc cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc   6120

tccggggtct tgccgttgta ccgcttgaac cactgagcgg cgggccgctc gatgccgtca   6180

ttgatccgct cggagatcat caggtggcag tgcgggttct cgccgccacc ggcatggatg   6240

gccagcgtat acggcaggcg ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc   6300

gccttctgct ggtcgagggt cagctcgacc ggcagggcaa attcgacctc cttgaacagc   6360

cgcccattgg cgcgttcata caggtcggca gcatcccagt agtcggcggg ccgctcgacg   6420

aactccggca tgtgcccgga ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg   6480

ccttcgcgct ggatgtagtc ggccttggcc ctggccgatt ggccgcccga cctgctgccg   6540

gttttcgccg taaggtgata aatcgccatg ctgcctcgct gttgcttttg cttttcggct   6600

ccatgcaatg gccctcggag agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg   6660

aagagaaacc ggtaagtgcg ccctccccta caaagtaggg tcgggattgc cgccgctgtg   6720

cctccatgat agcctacgag acagcacatt aacaatgggg tgtcaagatg gttaagggga   6780

gcaacaaggc ggcggatcgg ctggccaagc tcgaagaaca acgagcgcga atcaatgccg   6840

aaattcagcg ggtgcgggca agggaacagc agcaagagcg caagaacgaa acaaggcgca   6900

aggtgctggt gggggccatg attttggcca aggtgaacag cagcgagtgg ccggaggatc   6960

ggctcatggc ggcaatggat gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc   7020

tgccgccacg ccagaaggat gagccgggct gaatgatcga ccgagacagg ccctgcgggg   7080

ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc   7140

tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg gctttgcccg cctttccccc   7200

tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc   7260

tctggccggg catattgggc aagggcagca gcgccccaca agggcgctga taaccgcgcc   7320

tagtggatta ttcttagata atcatggatg gatttttcca acaccccgcc agcccccgcc   7380

cctgctgggt ttgcaggttt gggggcgtga cagttattgc aggggttcgt gacagttatt   7440

gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt tagtacggga gtgacgggca   7500

ctggctggca atgtctagca acggcaggca tttcggctga gggtaaaaga actttccgct   7560

aagcgataga ctgtatgtaa acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg   7620

gccaggacgg ccagccggga tcgggatact ggtcgttacc agagccaccg acccgagcaa   7680

acccttctct atcagatcgt tgacgagtat tacccggcat tcgctgcgct tatggcagag   7740

cagggaaagg aattgccggg ctatgtgcaa cgggaatttg aagaatttct ccaatgcggg   7800

cggctggagc atggctttct acgggttcgc tgcgagtctt gccacgccga gcacctggtc   7860

gctttcagct gtaatccggg cagcgcaacg gaacattcat cagtgtaaaa atggaatcaa   7920

taaagccctg cgcagcgcgc agggtcagcc tgaatacgcg tttaatgacc agcacagtcg   7980

tgatggcaag gtcagaatag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat   8040

accaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag   8100

ctttgttgta ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg   8160

cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac   8220

aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat   8280

catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata   8340

ttcaacggga aacgtcttgc tcgagaccga gctcgaattg gccgcggcgt tgtgacaatt   8400

taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg   8460

tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag   8520

ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt   8580

tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc   8640

cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ctgggcagaa   8700

cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg   8760

tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg   8820

ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt   8880

cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag   8940

ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac   9000

atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt   9060

acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt   9120

tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga   9180

accgaacagg cttatgtcaa ttcgagcatc gattgtatgg gaagcccgat gcgccagagt   9240

tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac   9300

taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg   9360

atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag   9420

aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc   9480

attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg   9540

cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg   9600

gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt   9660

cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa   9720

taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc   9780

tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg   9840

gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct   9900

aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg   9960

gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc  10020

cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta  10080

caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca  10140

ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgct attctgacct  10200

tgccatcacg actgtgctgg tcattaaacg cgtattcagg ctgaccctgc gcgctgcgca  10260

gggctttatt gattccattt ttacactgat gaatgttccg ttgcgctgcc cggattacag  10320

atcctctagc                                                         10330


