                         SEQUENCE LISTING

<110>  VIB VZW
       VRIJE UNIVERSITEIT BRUSSEL
       UNIVERSITEIT GENT
 
<120>  PROTECTIVE ANTI-ETEC ANTIBODY

<130>  ADP/ETEC/429

<150>  EP 12182774.5
<151>  2012-09-03

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  1092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion construct V1A


<220>
<221>  CDS
<222>  (1)..(1092)

<400>  1
cag gtg cag ctg cag gag tct gga gga ggc ttg gtg cag gct ggg ggg         48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly           
1               5                   10                  15                

tct ctg aga ctc tcc tgt gaa gcc tct gga aat gtc gac aga atc gat         96
Ser Leu Arg Leu Ser Cys Glu Ala Ser Gly Asn Val Asp Arg Ile Asp           
            20                  25                  30                    

gcc atg ggc tgg ttc cgc cag gct cca ggg aaa cag cgc gag ttc gtt        144
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val           
        35                  40                  45                        

ggt tat att agt gaa ggt ggt ata tta aac tat gga gac ttt gtg aag        192
Gly Tyr Ile Ser Glu Gly Gly Ile Leu Asn Tyr Gly Asp Phe Val Lys           
    50                  55                  60                            

ggc cga ttc acc atc tcc aga gac aac gcc aag aat acg gtg tat ctg        240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu           
65                  70                  75                  80            

caa atg agc aac ctg aaa tct gag gac aca ggc gtc tat ttt tgt gca        288
Gln Met Ser Asn Leu Lys Ser Glu Asp Thr Gly Val Tyr Phe Cys Ala           
                85                  90                  95                

gct agc cat tgg gga aca cta ctt atc aag gga ata gaa cac tgg ggc        336
Ala Ser His Trp Gly Thr Leu Leu Ile Lys Gly Ile Glu His Trp Gly           
            100                 105                 110                   

aaa ggc acc cag gtc acc gtc tcc tca gat cca tgt cct cag tgc tgc        384
Lys Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys           
        115                 120                 125                       

aag ccc agc ttg tcc ttg cag cca cca gct ctt gca gac ctc ctc ctc        432
Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu           
    130                 135                 140                           

gga tca aat gcc agc ctc acc tgc aca ctc agt gga ctc aaa aaa tca        480
Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser           
145                 150                 155                 160           

gag ggt gtc agc ttc act tgg caa ccc tca ggt ggg aag gat gct gtc        528
Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val           
                165                 170                 175               

caa gcg tcc ccc acg cgt gat tca tgt ggc tgc tac agc gtt tcc agc        576
Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser           
            180                 185                 190                   

att ttg cca ggc tgt gct gat cct tgg aac aaa gga gaa aca ttt tcc        624
Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser           
        195                 200                 205                       

tgc acc gct gcc cac tct gag ttg aag agc gcg cta acc gct acc atc        672
Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile           
    210                 215                 220                           

act aaa cct aaa gtt aac acg ttc aga ccc caa gtc cac ttg ttg ccg        720
Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro           
225                 230                 235                 240           

ccg ccg tcg gag gag ctc gct ctc aac gaa ctc gtt aca ctc aca tgc        768
Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys           
                245                 250                 255               

ctc gta aga ggc ttt agc cct aag gat gtg tta gtt cga tgg ctg caa        816
Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln           
            260                 265                 270                   

ggg ggc caa gaa ttg ccc cgt gac aag tac ctc gtc tgg gaa tcc ctt        864
Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu           
        275                 280                 285                       

cca gaa cct gga cag gct atc ccc acc tac gcc gtt acc agc gtg ctt        912
Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu           
    290                 295                 300                           

cgc gtt gac gcc gaa gac tgg aag cag gga gac acc ttc tcc tgc atg        960
Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met           
305                 310                 315                 320           

gta gga cac gag gct cta cct ctt gcc ttc acc cag aag acc atc gac       1008
Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp           
                325                 330                 335               

cgc ctt gcg ggt aaa ccc acc cac gtc aac gtg tca gtt gtc atg gcg       1056
Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala           
            340                 345                 350                   

gag gca gaa ggc ata tgc tac aaa gac gag ctt taa                       1092
Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu                               
        355                 360                                           


<210>  2
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Glu Ala Ser Gly Asn Val Asp Arg Ile Asp 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Gly Tyr Ile Ser Glu Gly Gly Ile Leu Asn Tyr Gly Asp Phe Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Ser Asn Leu Lys Ser Glu Asp Thr Gly Val Tyr Phe Cys Ala 
                85                  90                  95      


Ala Ser His Trp Gly Thr Leu Leu Ile Lys Gly Ile Glu His Trp Gly 
            100                 105                 110         


Lys Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys 
        115                 120                 125             


Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu 
    130                 135                 140                 


Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser 
145                 150                 155                 160 


Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val 
                165                 170                 175     


Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser 
            180                 185                 190         


Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser 
        195                 200                 205             


Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile 
    210                 215                 220                 


Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro 
225                 230                 235                 240 


Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys 
                245                 250                 255     


Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln 
            260                 265                 270         


Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu 
        275                 280                 285             


Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu 
    290                 295                 300                 


Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met 
305                 310                 315                 320 


Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp 
                325                 330                 335     


Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala 
            340                 345                 350         


Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu 
        355                 360             


<210>  3
<211>  1092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion construct V2A


<220>
<221>  CDS
<222>  (1)..(1092)

<400>  3
cag gtg cag ctg cag gag tct gga gga ggc ttg gtg cag cct ggg ggg         48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly           
1               5                   10                  15                

tct ctg aga ctc tcc tgt aca gcc tcc gga agc atc tcc agt atc aat         96
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Ser Ile Ser Ser Ile Asn           
            20                  25                  30                    

gcc atg ggc tgg tac cgc cag gct cca ggg agc aag cgc gag ttc gtc        144
Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Ser Lys Arg Glu Phe Val           
        35                  40                  45                        

gca cat att act aac acg ggt gtg aca gaa ttt gca gac tcc gtg aag        192
Ala His Ile Thr Asn Thr Gly Val Thr Glu Phe Ala Asp Ser Val Lys           
    50                  55                  60                            

ggc cga ttc acc atc tcc aga gac aac gcc aag acc acg gtg gat ctg        240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr Val Asp Leu           
65                  70                  75                  80            

caa atg aac agc ctg aaa cct gag gac aca gcc gtc tat tac tgt gca        288
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala           
                85                  90                  95                

gct act gat tgg ggc acc cta ctt atc aag ggc att gac cac tgg ggt        336
Ala Thr Asp Trp Gly Thr Leu Leu Ile Lys Gly Ile Asp His Trp Gly           
            100                 105                 110                   

aaa ggg acc cag gtc acc gtc tcc tca gat cca tgt cct cag tgc tgc        384
Lys Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys           
        115                 120                 125                       

aag ccc agc ttg tcc ttg cag cca cca gct ctt gca gac ctc ctc ctc        432
Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu           
    130                 135                 140                           

gga tca aat gcc agc ctc acc tgc aca ctc agt gga ctc aaa aaa tca        480
Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser           
145                 150                 155                 160           

gag ggt gtc agc ttc act tgg caa ccc tca ggt ggg aag gat gct gtc        528
Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val           
                165                 170                 175               

caa gcg tcc ccc acg cgt gat tca tgt ggc tgc tac agc gtt tcc agc        576
Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser           
            180                 185                 190                   

att ttg cca ggc tgt gct gat cct tgg aac aaa gga gaa aca ttt tcc        624
Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser           
        195                 200                 205                       

tgc acc gct gcc cac tct gag ttg aag agc gcg cta acc gct acc atc        672
Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile           
    210                 215                 220                           

act aaa cct aaa gtt aac acg ttc aga ccc caa gtc cac ttg ttg ccg        720
Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro           
225                 230                 235                 240           

ccg ccg tcg gag gag ctc gct ctc aac gaa ctc gtt aca ctc aca tgc        768
Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys           
                245                 250                 255               

ctc gta aga ggc ttt agc cct aag gat gtg tta gtt cga tgg ctg caa        816
Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln           
            260                 265                 270                   

ggg ggc caa gaa ttg ccc cgt gac aag tac ctc gtc tgg gaa tcc ctt        864
Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu           
        275                 280                 285                       

cca gaa cct gga cag gct atc ccc acc tac gcc gtt acc agc gtg ctt        912
Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu           
    290                 295                 300                           

cgc gtt gac gcc gaa gac tgg aag cag gga gac acc ttc tcc tgc atg        960
Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met           
305                 310                 315                 320           

gta gga cac gag gct cta cct ctt gcc ttc acc cag aag acc atc gac       1008
Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp           
                325                 330                 335               

cgc ctt gcg ggt aaa ccc acc cac gtc aac gtg tca gtt gtc atg gcg       1056
Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala           
            340                 345                 350                   

gag gca gaa ggc ata tgc tac aaa gac gag ctt taa                       1092
Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu                               
        355                 360                                           


<210>  4
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Ser Ile Ser Ser Ile Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Ser Lys Arg Glu Phe Val 
        35                  40                  45              


Ala His Ile Thr Asn Thr Gly Val Thr Glu Phe Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr Val Asp Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Thr Asp Trp Gly Thr Leu Leu Ile Lys Gly Ile Asp His Trp Gly 
            100                 105                 110         


Lys Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys 
        115                 120                 125             


Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu 
    130                 135                 140                 


Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser 
145                 150                 155                 160 


Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val 
                165                 170                 175     


Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser 
            180                 185                 190         


Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser 
        195                 200                 205             


Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile 
    210                 215                 220                 


Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro 
225                 230                 235                 240 


Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys 
                245                 250                 255     


Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln 
            260                 265                 270         


Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu 
        275                 280                 285             


Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu 
    290                 295                 300                 


Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met 
305                 310                 315                 320 


Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp 
                325                 330                 335     


Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala 
            340                 345                 350         


Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu 
        355                 360             


<210>  5
<211>  1092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion construct V3A


<220>
<221>  CDS
<222>  (1)..(1092)

<400>  5
cag gtg cag ctg cag gag tct gga gga ggc ttg gtg cag gct ggg ggg         48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly           
1               5                   10                  15                

tct ctg aga ctc tcc tgt gca gcc tcg ggc ctt acc ttc gat act tat         96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Asp Thr Tyr           
            20                  25                  30                    

gcc atg ggc tgg ttc cgc cag gct cca ggg aag aag cgt gag tat gta        144
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Lys Arg Glu Tyr Val           
        35                  40                  45                        

gca gct att agc tgg acc ggt att agc aca tat tat gca gac atc gcg        192
Ala Ala Ile Ser Trp Thr Gly Ile Ser Thr Tyr Tyr Ala Asp Ile Ala           
    50                  55                  60                            

aag ggc cga ttc acc atc tcc aga gac aac gcc aag aac acg ctg tat        240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr           
65                  70                  75                  80            

cta caa atg gat agc ctg aaa cct gag gac acg gcc gtt tat tac tgt        288
Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

gca gcc caa aaa tcc ctc aac gta cca gcg ccg tgg gac tat tgg ggc        336
Ala Ala Gln Lys Ser Leu Asn Val Pro Ala Pro Trp Asp Tyr Trp Gly           
            100                 105                 110                   

cag ggg acc cag gtc acc gtc tcc tca gat cca tgt cct cag tgc tgc        384
Gln Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys           
        115                 120                 125                       

aag ccc agc ttg tcc ttg cag cca cca gct ctt gca gac ctc ctc ctc        432
Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu           
    130                 135                 140                           

gga tca aat gcc agc ctc acc tgc aca ctc agt gga ctc aaa aaa tca        480
Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser           
145                 150                 155                 160           

gag ggt gtc agc ttc act tgg caa ccc tca ggt ggg aag gat gct gtc        528
Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val           
                165                 170                 175               

caa gcg tcc ccc acg cgt gat tca tgt ggc tgc tac agc gtt tcc agc        576
Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser           
            180                 185                 190                   

att ttg cca ggc tgt gct gat cct tgg aac aaa gga gaa aca ttt tcc        624
Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser           
        195                 200                 205                       

tgc acc gct gcc cac tct gag ttg aag agc gcg cta acc gct acc atc        672
Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile           
    210                 215                 220                           

act aaa cct aaa gtt aac acg ttc aga ccc caa gtc cac ttg ttg ccg        720
Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro           
225                 230                 235                 240           

ccg ccg tcg gag gag ctc gct ctc aac gaa ctc gtt aca ctc aca tgc        768
Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys           
                245                 250                 255               

ctc gta aga ggc ttt agc cct aag gat gtg tta gtt cga tgg ctg caa        816
Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln           
            260                 265                 270                   

ggg ggc caa gaa ttg ccc cgt gac aag tac ctc gtc tgg gaa tcc ctt        864
Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu           
        275                 280                 285                       

cca gaa cct gga cag gct atc ccc acc tac gcc gtt acc agc gtg ctt        912
Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu           
    290                 295                 300                           

cgc gtt gac gcc gaa gac tgg aag cag gga gac acc ttc tcc tgc atg        960
Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met           
305                 310                 315                 320           

gta gga cac gag gct cta cct ctt gcc ttc acc cag aag acc atc gac       1008
Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp           
                325                 330                 335               

cgc ctt gcg ggt aaa ccc acc cac gtc aac gtg tca gtt gtc atg gcg       1056
Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala           
            340                 345                 350                   

gag gca gaa ggc ata tgc tac aaa gac gag ctt taa                       1092
Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu                               
        355                 360                                           


<210>  6
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Asp Thr Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Lys Arg Glu Tyr Val 
        35                  40                  45              


Ala Ala Ile Ser Trp Thr Gly Ile Ser Thr Tyr Tyr Ala Asp Ile Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Lys Ser Leu Asn Val Pro Ala Pro Trp Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys 
        115                 120                 125             


Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu 
    130                 135                 140                 


Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser 
145                 150                 155                 160 


Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val 
                165                 170                 175     


Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser 
            180                 185                 190         


Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser 
        195                 200                 205             


Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile 
    210                 215                 220                 


Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro 
225                 230                 235                 240 


Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys 
                245                 250                 255     


Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln 
            260                 265                 270         


Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu 
        275                 280                 285             


Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu 
    290                 295                 300                 


Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met 
305                 310                 315                 320 


Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp 
                325                 330                 335     


Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala 
            340                 345                 350         


Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu 
        355                 360             


<210>  7
<211>  1092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion construct V4A


<220>
<221>  CDS
<222>  (1)..(1092)

<400>  7
cag gtg cag ctg cag gag tct gga gga gga ttg gtg cag gct ggg ggc         48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly           
1               5                   10                  15                

tct ctg aga ctc tcc tgt gca gcc tcg ggc ctt acc ttc gat act tat         96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Asp Thr Tyr           
            20                  25                  30                    

gcc atg ggc tgg ttc cgc cag gct cca ggg aag aag cgt gag tat gta        144
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Lys Arg Glu Tyr Val           
        35                  40                  45                        

gca gcg att agc tgg acc ggt att agc aca tat tat gca gac atc gcg        192
Ala Ala Ile Ser Trp Thr Gly Ile Ser Thr Tyr Tyr Ala Asp Ile Ala           
    50                  55                  60                            

aag ggc cga ttc acc atc tcc aga gac aac gcc aag aac acg ctg tat        240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr           
65                  70                  75                  80            

ctt caa atg gac agc ctg aaa cct gag gac acg gcc gtt tat tac tgt        288
Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

gca gcc caa aga tcc ctc aac gta cca gcg ccg tgg gac tat tgg ggc        336
Ala Ala Gln Arg Ser Leu Asn Val Pro Ala Pro Trp Asp Tyr Trp Gly           
            100                 105                 110                   

cag ggg acc cag gtc acc gtc tcc tca gat cca tgt cct cag tgc tgc        384
Gln Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys           
        115                 120                 125                       

aag ccc agc ttg tcc ttg cag cca cca gct ctt gca gac ctc ctc ctc        432
Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu           
    130                 135                 140                           

gga tca aat gcc agc ctc acc tgc aca ctc agt gga ctc aaa aaa tca        480
Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser           
145                 150                 155                 160           

gag ggt gtc agc ttc act tgg caa ccc tca ggt ggg aag gat gct gtc        528
Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val           
                165                 170                 175               

caa gcg tcc ccc acg cgt gat tca tgt ggc tgc tac agc gtt tcc agc        576
Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser           
            180                 185                 190                   

att ttg cca ggc tgt gct gat cct tgg aac aaa gga gaa aca ttt tcc        624
Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser           
        195                 200                 205                       

tgc acc gct gcc cac tct gag ttg aag agc gcg cta acc gct acc atc        672
Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile           
    210                 215                 220                           

act aaa cct aaa gtt aac acg ttc aga ccc caa gtc cac ttg ttg ccg        720
Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro           
225                 230                 235                 240           

ccg ccg tcg gag gag ctc gct ctc aac gaa ctc gtt aca ctc aca tgc        768
Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys           
                245                 250                 255               

ctc gta aga ggc ttt agc cct aag gat gtg tta gtt cga tgg ctg caa        816
Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln           
            260                 265                 270                   

ggg ggc caa gaa ttg ccc cgt gac aag tac ctc gtc tgg gaa tcc ctt        864
Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu           
        275                 280                 285                       

cca gaa cct gga cag gct atc ccc acc tac gcc gtt acc agc gtg ctt        912
Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu           
    290                 295                 300                           

cgc gtt gac gcc gaa gac tgg aag cag gga gac acc ttc tcc tgc atg        960
Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met           
305                 310                 315                 320           

gta gga cac gag gct cta cct ctt gcc ttc acc cag aag acc atc gac       1008
Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp           
                325                 330                 335               

cgc ctt gcg ggt aaa ccc acc cac gtc aac gtg tca gtt gtc atg gcg       1056
Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala           
            340                 345                 350                   

gag gca gaa ggc ata tgc tac aaa gac gag ctt taa                       1092
Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu                               
        355                 360                                           


<210>  8
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Asp Thr Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Lys Arg Glu Tyr Val 
        35                  40                  45              


Ala Ala Ile Ser Trp Thr Gly Ile Ser Thr Tyr Tyr Ala Asp Ile Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Arg Ser Leu Asn Val Pro Ala Pro Trp Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Asp Pro Cys Pro Gln Cys Cys 
        115                 120                 125             


Lys Pro Ser Leu Ser Leu Gln Pro Pro Ala Leu Ala Asp Leu Leu Leu 
    130                 135                 140                 


Gly Ser Asn Ala Ser Leu Thr Cys Thr Leu Ser Gly Leu Lys Lys Ser 
145                 150                 155                 160 


Glu Gly Val Ser Phe Thr Trp Gln Pro Ser Gly Gly Lys Asp Ala Val 
                165                 170                 175     


Gln Ala Ser Pro Thr Arg Asp Ser Cys Gly Cys Tyr Ser Val Ser Ser 
            180                 185                 190         


Ile Leu Pro Gly Cys Ala Asp Pro Trp Asn Lys Gly Glu Thr Phe Ser 
        195                 200                 205             


Cys Thr Ala Ala His Ser Glu Leu Lys Ser Ala Leu Thr Ala Thr Ile 
    210                 215                 220                 


Thr Lys Pro Lys Val Asn Thr Phe Arg Pro Gln Val His Leu Leu Pro 
225                 230                 235                 240 


Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Val Thr Leu Thr Cys 
                245                 250                 255     


Leu Val Arg Gly Phe Ser Pro Lys Asp Val Leu Val Arg Trp Leu Gln 
            260                 265                 270         


Gly Gly Gln Glu Leu Pro Arg Asp Lys Tyr Leu Val Trp Glu Ser Leu 
        275                 280                 285             


Pro Glu Pro Gly Gln Ala Ile Pro Thr Tyr Ala Val Thr Ser Val Leu 
    290                 295                 300                 


Arg Val Asp Ala Glu Asp Trp Lys Gln Gly Asp Thr Phe Ser Cys Met 
305                 310                 315                 320 


Val Gly His Glu Ala Leu Pro Leu Ala Phe Thr Gln Lys Thr Ile Asp 
                325                 330                 335     


Arg Leu Ala Gly Lys Pro Thr His Val Asn Val Ser Val Val Met Ala 
            340                 345                 350         


Glu Ala Glu Gly Ile Cys Tyr Lys Asp Glu Leu 
        355                 360             


<210>  9
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  J chain

<400>  9
caggatgaag aagaaagtac cgtccttgtt gataacaaat gcaagtgcgc ccgtataact       60

tccaggatca tccgctctgc tgaagaccct tcacaagaca ttgtagagag aaacatcaga      120

attattgttc ctctcaacaa cagggagaac atcagcgatc ccacctcacc actccgaacc      180

aatttcgtgt accatttgag cgacctctgt aagaaatgcg atcttacaga agttgagctc      240

gataatcaaa tcgttactgc aacccagagc aacctatgtg atgacgacat tgagacctgc      300

tacgcttacg acagaaacaa gtgctacaca aacacggtcc cattcaccta cgatggacag      360

accaaaatgg tgcaaacagc cttgaccccg gattcctgct atcctgacaa agacgagctt      420

taa                                                                    423


<210>  10
<211>  1752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secretory component

<400>  10
aaaagtccca tattcggtcc ccaggatgtt agcagcgttg aaggtagctc agtgtccatc       60

agatgctact acccagccac ctccgtcaac cgtcattctc gtaaatactg gtgccgaata      120

ggagccaaag gccgctgcac aacgctcatc tcctcagagg gttacatctc caaggattac      180

aaaggcagag ccaacctcac caacttccca gagaacggca ccttcgtgat ggatattggt      240

cacttgacgc gcggtgattc tggactctac aaatgtggtc tcggcattag cagccgaggt      300

ctctctttcg atgtttcact cgaggtctca caaggtcctg gacagatagg tgatgtccac      360

gtctacacag cagatttggg ttcaacagta accatcaact gccctttcaa atctgagaat      420

gctcagaagc caaaatccgt ttacaaaaaa ttgggccaga tccgcgtcct cgtcatcgat      480

tccaatgggt atttgaacaa caactttacc aacagagcac atctcagtat tcagggtacc      540

aaccaattag tattcagctt cgtcatcaac cgaatccagc tccgcgatgc tggaatatac      600

atctgccagg ctggagatga agagagcagc gctgacctcc aagttttgaa gcccgaacct      660

gaacttattt atggagacct taggggctca gttacatttg actgcgccct tggccaggaa      720

atggcaaatg ttgccaaatt cctttgccaa ctaaaaaacg gaaaaacctg caatgttgtc      780

atcaacaccc ttggaaagaa ggctcaggac ttcgaaggca ggatccttct cactcccaag      840

gaaaacagcc acttcagcgt tcacatcacg ggacttcgta aagaagacgc aggacactac      900

ctttgcggag cccaccctga cggtgagcct aaggaaggct ggccagtcca ggcttggcag      960

ctcttcatca acgaagatac tatgattccc ccaagatcct ccgttgtgag aggtgttgtg     1020

ggaggctccg tggccgtgac ctgcccctac aacccgaagg aaacaaacag cctgaagtac     1080

tggtgtcgct gggaagagaa tgaaaacggt cgctgcccgc aacttgttga aagcagcggg     1140

ctagttaacg agcaatacga gggtagactc gcgctctacg aggagccagg caacggtacc     1200

ttcacggtca tactcaacca actcaccaac agagacgccg gcttctactg gtgtttgacc     1260

aacgaggact ctcgctggag gtccacggta gaactcaaga ttgttgaagg agaaccgaac     1320

ctcaagctac ccgagaacgt caccgcttgg gttggagaaa ccctcaagct ctcctgccac     1380

ttcccatgca aattctactc ctaccaaaag tactggtgta agtggagcaa caccggttgc     1440

agggcccttc ccagccaaga cgaaggtcaa agccaagcct ttgttaactg tgacaagaag     1500

agccaaatca tctccttgaa cttgaaccct gtcagaaagg aggatgaagg ctggtactgg     1560

tgcggggtga aggacggact ccactatgga gagactgggg ctgtctacgt ggcagtggaa     1620

cagaaggcaa aggggtcagg agatgcccgt ctagcaaacg ctgctcctgc tcctgctgaa     1680

gacgcaatag agccaagggc cagggagact gagaacgagg tcctcttgga tcccagcaaa     1740

gacgagcttt aa                                                         1752


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
gtgccttggg tgagaggtta                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
cactctgccg ttctctttcc                                                   20


