                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  Yeast Strains engineered to produce ethanol from acetate

<130>  29037-WO-PCT

<150> EP12181937.9 
<151> 2012-08-28

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  80
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer GPD1-pSUC227

<400>  1
gaaacaattg tatattgtac accccccccc tccacaaaca caaatattga taatataaag       60

tcgacctcga gtaccgttcg                                                   80


<210>  2
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer pSUC227

<400>  2
gcaatttcgg ctatacgtaa c                                                 21


<210>  3
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer pSUC225

<400>  3
cgttcactca tggaaaatag c                                                 21


<210>  4
<211>  81
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer GPD1-pSUC225

<400>  4
gaagagcctc gaaaaaagtg ggggaaagta tgatatgtta tctttctcca ataaatctaa       60

cggatcctac cgttcgtata g                                                 81


<210>  5
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer GPD1

<400>  5
cgtagactgg cttggtattg g                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer GPD1

<400>  6
gcctctgaat gagtggtgtt g                                                 21


<210>  7
<211>  80
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer GPD2-pSUC227

<400>  7
gtattttggt agattcaatt ctctttccct ttccttttcc ttcgctcccc ttccttatca       60

tcgacctcga gtaccgttcg                                                   80


<210>  8
<211>  81
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer GPD2-pSUC225

<400>  8
agagtgtcta ttcgtcatcg atgtctagct cttcaatcat ctccggtagg tcttccatgc       60

cggatcctac cgttcgtata g                                                 81


<210>  9
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer GPD2

<400>  9
cttgggttct tctttctact cc                                                22


<210>  10
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>   reverse primer GPD2

<400>  10
cgtatctttc attaacggtg atgtg                                             25


<210>  11
<211>  1404
<212>  DNA
<213>  nucleotide sequence aldehyde oxidoreductase (Escherichia coli)

<400>  11
atgaaccaac aagatatcga acaagttgtc aaggctgtct tgttgaaaat gcaatcttct       60

gacactccat ctgctgctgt ccacgaaatg ggtgttttcg cttctttgga cgacgctgtt      120

gctgctgcca aggttgctca acaaggtttg aaatctgttg ccatgagaca attggccatt      180

gctgccatca gagaagctgg tgaaaagcat gccagagact tggctgaatt ggctgtctcc      240

gaaaccggta tgggtagagt tgaagacaaa ttcgctaaga acgttgctca agctagaggt      300

actccaggtg tcgaatgttt gtctccacaa gtcttgaccg gtgataatgg tttgactttg      360

attgaaaatg ctccatgggg tgttgttgct tccgtcaccc catctaccaa cccagctgct      420

actgtcatca acaacgccat ctctttgatt gctgctggta actccgttat cttcgctcca      480

cacccagctg ccaagaaggt ttctcaaaga gccatcactc tattgaacca agccattgtt      540

gctgctggtg gtccagaaaa cttgttggtc actgttgcca acccagatat cgaaactgct      600

caaagattat tcaagttccc aggtatcggt ctattagtcg tcactggtgg tgaagctgtt      660

gttgaagctg ccagaaagca caccaacaag agattgattg ctgctggtgc tggtaaccct      720

cctgttgttg tcgatgaaac cgctgatttg gccagagctg ctcaatccat tgtcaagggt      780

gcttctttcg acaacaacat catctgtgct gacgaaaagg ttttgattgt tgttgactcc      840

gttgctgacg aattgatgag attgatggaa ggtcaacatg ccgtcaagtt gactgctgaa      900

caagctcaac aattgcaacc agttttgttg aagaacatcg atgaaagagg taagggtacc      960

gtctccagag actgggttgg tagagatgct ggtaagattg ctgctgccat cggtttgaag     1020

gttccacaag aaaccagatt attattcgtc gaaaccaccg ctgaacaccc atttgctgtc     1080

actgaattga tgatgccagt cttaccagtt gtccgtgttg ctaacgttgc tgacgctatt     1140

gctttggctg tcaaattgga aggtggttgt caccacactg ctgccatgca ctccagaaac     1200

atcgaaaaca tgaaccaaat ggctaacgcc attgacactt ccatctttgt caagaacggt     1260

ccatgtatcg ctggtttggg tttgggtggt gaaggttgga ccaccatgac catcaccacc     1320

ccaactggtg aaggtgtcac ttctgccaga actttcgtca gattacgtcg ttgtgttttg     1380

gtcgatgctt tcagaattgt ttaa                                            1404


<210>  12
<211>  467
<212>  PRT
<213>  amino acid sequence aldehyde oxidoreductase (Escherichia coli)

<400>  12

Met Asn Gln Gln Asp Ile Glu Gln Val Val Lys Ala Val Leu Leu Lys 
1               5                   10                  15      


Met Gln Ser Ser Asp Thr Pro Ser Ala Ala Val His Glu Met Gly Val 
            20                  25                  30          


Phe Ala Ser Leu Asp Asp Ala Val Ala Ala Ala Lys Val Ala Gln Gln 
        35                  40                  45              


Gly Leu Lys Ser Val Ala Met Arg Gln Leu Ala Ile Ala Ala Ile Arg 
    50                  55                  60                  


Glu Ala Gly Glu Lys His Ala Arg Asp Leu Ala Glu Leu Ala Val Ser 
65                  70                  75                  80  


Glu Thr Gly Met Gly Arg Val Glu Asp Lys Phe Ala Lys Asn Val Ala 
                85                  90                  95      


Gln Ala Arg Gly Thr Pro Gly Val Glu Cys Leu Ser Pro Gln Val Leu 
            100                 105                 110         


Thr Gly Asp Asn Gly Leu Thr Leu Ile Glu Asn Ala Pro Trp Gly Val 
        115                 120                 125             


Val Ala Ser Val Thr Pro Ser Thr Asn Pro Ala Ala Thr Val Ile Asn 
    130                 135                 140                 


Asn Ala Ile Ser Leu Ile Ala Ala Gly Asn Ser Val Ile Phe Ala Pro 
145                 150                 155                 160 


His Pro Ala Ala Lys Lys Val Ser Gln Arg Ala Ile Thr Leu Leu Asn 
                165                 170                 175     


Gln Ala Ile Val Ala Ala Gly Gly Pro Glu Asn Leu Leu Val Thr Val 
            180                 185                 190         


Ala Asn Pro Asp Ile Glu Thr Ala Gln Arg Leu Phe Lys Phe Pro Gly 
        195                 200                 205             


Ile Gly Leu Leu Val Val Thr Gly Gly Glu Ala Val Val Glu Ala Ala 
    210                 215                 220                 


Arg Lys His Thr Asn Lys Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro 
225                 230                 235                 240 


Pro Val Val Val Asp Glu Thr Ala Asp Leu Ala Arg Ala Ala Gln Ser 
                245                 250                 255     


Ile Val Lys Gly Ala Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu 
            260                 265                 270         


Lys Val Leu Ile Val Val Asp Ser Val Ala Asp Glu Leu Met Arg Leu 
        275                 280                 285             


Met Glu Gly Gln His Ala Val Lys Leu Thr Ala Glu Gln Ala Gln Gln 
    290                 295                 300                 


Leu Gln Pro Val Leu Leu Lys Asn Ile Asp Glu Arg Gly Lys Gly Thr 
305                 310                 315                 320 


Val Ser Arg Asp Trp Val Gly Arg Asp Ala Gly Lys Ile Ala Ala Ala 
                325                 330                 335     


Ile Gly Leu Lys Val Pro Gln Glu Thr Arg Leu Leu Phe Val Glu Thr 
            340                 345                 350         


Thr Ala Glu His Pro Phe Ala Val Thr Glu Leu Met Met Pro Val Leu 
        355                 360                 365             


Pro Val Val Arg Val Ala Asn Val Ala Asp Ala Ile Ala Leu Ala Val 
    370                 375                 380                 


Lys Leu Glu Gly Gly Cys His His Thr Ala Ala Met His Ser Arg Asn 
385                 390                 395                 400 


Ile Glu Asn Met Asn Gln Met Ala Asn Ala Ile Asp Thr Ser Ile Phe 
                405                 410                 415     


Val Lys Asn Gly Pro Cys Ile Ala Gly Leu Gly Leu Gly Gly Glu Gly 
            420                 425                 430         


Trp Thr Thr Met Thr Ile Thr Thr Pro Thr Gly Glu Gly Val Thr Ser 
        435                 440                 445             


Ala Arg Thr Phe Val Arg Leu Arg Arg Cys Val Leu Val Asp Ala Phe 
    450                 455                 460                 


Arg Ile Val 
465         


<210>  13
<211>  71
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer KanMX TRP1

<400>  13
taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac cacagacgcg       60

ttgaattgtc c                                                            71


<210>  14
<211>  72
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer KanMX TRP1

<400>  14
aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac tcgcatctgg       60

gcagatgatg tc                                                           72


<210>  15
<211>  71
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer KanMX URA3

<400>  15
taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc cacagacgcg       60

ttgaattgtc c                                                            71


<210>  16
<211>  72
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer KanMX URA3

<400>  16
agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tcgcatctgg       60

gcagatgatg tc                                                           72


<210>  17
<211>  391
<212>  PRT
<213>  Saccharomyces cerevisiae GPD1 protein sequence

<400>  17

Met Ser Ala Ala Ala Asp Arg Leu Asn Leu Thr Ser Gly His Leu Asn 
1               5                   10                  15      


Ala Gly Arg Lys Arg Ser Ser Ser Ser Val Ser Leu Lys Ala Ala Glu 
            20                  25                  30          


Lys Pro Phe Lys Val Thr Val Ile Gly Ser Gly Asn Trp Gly Thr Thr 
        35                  40                  45              


Ile Ala Lys Val Val Ala Glu Asn Cys Lys Gly Tyr Pro Glu Val Phe 
    50                  55                  60                  


Ala Pro Ile Val Gln Met Trp Val Phe Glu Glu Glu Ile Asn Gly Glu 
65                  70                  75                  80  


Lys Leu Thr Glu Ile Ile Asn Thr Arg His Gln Asn Val Lys Tyr Leu 
                85                  90                  95      


Pro Gly Ile Thr Leu Pro Asp Asn Leu Val Ala Asn Pro Asp Leu Ile 
            100                 105                 110         


Asp Ser Val Lys Asp Val Asp Ile Ile Val Phe Asn Ile Pro His Gln 
        115                 120                 125             


Phe Leu Pro Arg Ile Cys Ser Gln Leu Lys Gly His Val Asp Ser His 
    130                 135                 140                 


Val Arg Ala Ile Ser Cys Leu Lys Gly Phe Glu Val Gly Ala Lys Gly 
145                 150                 155                 160 


Val Gln Leu Leu Ser Ser Tyr Ile Thr Glu Glu Leu Gly Ile Gln Cys 
                165                 170                 175     


Gly Ala Leu Ser Gly Ala Asn Ile Ala Thr Glu Val Ala Gln Glu His 
            180                 185                 190         


Trp Ser Glu Thr Thr Val Ala Tyr His Ile Pro Lys Asp Phe Arg Gly 
        195                 200                 205             


Glu Gly Lys Asp Val Asp His Lys Val Leu Lys Ala Leu Phe His Arg 
    210                 215                 220                 


Pro Tyr Phe His Val Ser Val Ile Glu Asp Val Ala Gly Ile Ser Ile 
225                 230                 235                 240 


Cys Gly Ala Leu Lys Asn Val Val Ala Leu Gly Cys Gly Phe Val Glu 
                245                 250                 255     


Gly Leu Gly Trp Gly Asn Asn Ala Ser Ala Ala Ile Gln Arg Val Gly 
            260                 265                 270         


Leu Gly Glu Ile Ile Arg Phe Gly Gln Met Phe Phe Pro Glu Ser Arg 
        275                 280                 285             


Glu Glu Thr Tyr Tyr Gln Glu Ser Ala Gly Val Ala Asp Leu Ile Thr 
    290                 295                 300                 


Thr Cys Ala Gly Gly Arg Asn Val Lys Val Ala Arg Leu Met Ala Thr 
305                 310                 315                 320 


Ser Gly Lys Asp Ala Trp Glu Cys Glu Lys Glu Leu Leu Asn Gly Gln 
                325                 330                 335     


Ser Ala Gln Gly Leu Ile Thr Cys Lys Glu Val His Glu Trp Leu Glu 
            340                 345                 350         


Thr Cys Gly Ser Val Glu Asp Phe Pro Leu Phe Glu Ala Val Tyr Gln 
        355                 360                 365             


Ile Val Tyr Asn Asn Tyr Pro Met Lys Asn Leu Pro Asp Met Ile Glu 
    370                 375                 380                 


Glu Leu Asp Leu His Glu Asp 
385                 390     


<210>  18
<211>  440
<212>  PRT
<213>  Saccharomyces cerevisiae GPD2 protein sequence

<400>  18

Met Leu Ala Val Arg Arg Leu Thr Arg Tyr Thr Phe Leu Lys Arg Thr 
1               5                   10                  15      


His Pro Val Leu Tyr Thr Arg Arg Ala Tyr Lys Ile Leu Pro Ser Arg 
            20                  25                  30          


Ser Thr Phe Leu Arg Arg Ser Leu Leu Gln Thr Gln Leu His Ser Lys 
        35                  40                  45              


Met Thr Ala His Thr Asn Ile Lys Gln His Lys His Cys His Glu Asp 
    50                  55                  60                  


His Pro Ile Arg Arg Ser Asp Ser Ala Val Ser Ile Val His Leu Lys 
65                  70                  75                  80  


Arg Ala Pro Phe Lys Val Thr Val Ile Gly Ser Gly Asn Trp Gly Thr 
                85                  90                  95      


Thr Ile Ala Lys Val Ile Ala Glu Asn Thr Glu Leu His Ser His Ile 
            100                 105                 110         


Phe Glu Pro Glu Val Arg Met Trp Val Phe Asp Glu Lys Ile Gly Asp 
        115                 120                 125             


Glu Asn Leu Thr Asp Ile Ile Asn Thr Arg His Gln Asn Val Lys Tyr 
    130                 135                 140                 


Leu Pro Asn Ile Asp Leu Pro His Asn Leu Val Ala Asp Pro Asp Leu 
145                 150                 155                 160 


Leu His Ser Ile Lys Gly Ala Asp Ile Leu Val Phe Asn Ile Pro His 
                165                 170                 175     


Gln Phe Leu Pro Asn Ile Val Lys Gln Leu Gln Gly His Val Ala Pro 
            180                 185                 190         


His Val Arg Ala Ile Ser Cys Leu Lys Gly Phe Glu Leu Gly Ser Lys 
        195                 200                 205             


Gly Val Gln Leu Leu Ser Ser Tyr Val Thr Asp Glu Leu Gly Ile Gln 
    210                 215                 220                 


Cys Gly Ala Leu Ser Gly Ala Asn Leu Ala Pro Glu Val Ala Lys Glu 
225                 230                 235                 240 


His Trp Ser Glu Thr Thr Val Ala Tyr Gln Leu Pro Lys Asp Tyr Gln 
                245                 250                 255     


Gly Asp Gly Lys Asp Val Asp His Lys Ile Leu Lys Leu Leu Phe His 
            260                 265                 270         


Arg Pro Tyr Phe His Val Asn Val Ile Asp Asp Val Ala Gly Ile Ser 
        275                 280                 285             


Ile Ala Gly Ala Leu Lys Asn Val Val Ala Leu Ala Cys Gly Phe Val 
    290                 295                 300                 


Glu Gly Met Gly Trp Gly Asn Asn Ala Ser Ala Ala Ile Gln Arg Leu 
305                 310                 315                 320 


Gly Leu Gly Glu Ile Ile Lys Phe Gly Arg Met Phe Phe Pro Glu Ser 
                325                 330                 335     


Lys Val Glu Thr Tyr Tyr Gln Glu Ser Ala Gly Val Ala Asp Leu Ile 
            340                 345                 350         


Thr Thr Cys Ser Gly Gly Arg Asn Val Lys Val Ala Thr Tyr Met Ala 
        355                 360                 365             


Lys Thr Gly Lys Ser Ala Leu Glu Ala Glu Lys Glu Leu Leu Asn Gly 
    370                 375                 380                 


Gln Ser Ala Gln Gly Ile Ile Thr Cys Arg Glu Val His Glu Trp Leu 
385                 390                 395                 400 


Gln Thr Cys Glu Leu Thr Gln Glu Phe Pro Leu Phe Glu Ala Val Tyr 
                405                 410                 415     


Gln Ile Val Tyr Asn Asn Val Arg Met Glu Asp Leu Pro Glu Met Ile 
            420                 425                 430         


Glu Glu Leu Asp Ile Asp Asp Glu 
        435                 440 


<210>  19
<211>  713
<212>  PRT
<213>  Saccharomyces cerevisiae ACS1 protein sequence

<400>  19

Met Ser Pro Ser Ala Val Gln Ser Ser Lys Leu Glu Glu Gln Ser Ser 
1               5                   10                  15      


Glu Ile Asp Lys Leu Lys Ala Lys Met Ser Gln Ser Ala Ala Thr Ala 
            20                  25                  30          


Gln Gln Lys Lys Glu His Glu Tyr Glu His Leu Thr Ser Val Lys Ile 
        35                  40                  45              


Val Pro Gln Arg Pro Ile Ser Asp Arg Leu Gln Pro Ala Ile Ala Thr 
    50                  55                  60                  


His Tyr Ser Pro His Leu Asp Gly Leu Gln Asp Tyr Gln Arg Leu His 
65                  70                  75                  80  


Lys Glu Ser Ile Glu Asp Pro Ala Lys Phe Phe Gly Ser Lys Ala Thr 
                85                  90                  95      


Gln Phe Leu Asn Trp Ser Lys Pro Phe Asp Lys Val Phe Ile Pro Asp 
            100                 105                 110         


Pro Lys Thr Gly Arg Pro Ser Phe Gln Asn Asn Ala Trp Phe Leu Asn 
        115                 120                 125             


Gly Gln Leu Asn Ala Cys Tyr Asn Cys Val Asp Arg His Ala Leu Lys 
    130                 135                 140                 


Thr Pro Asn Lys Lys Ala Ile Ile Phe Glu Gly Asp Glu Pro Gly Gln 
145                 150                 155                 160 


Gly Tyr Ser Ile Thr Tyr Lys Glu Leu Leu Glu Glu Val Cys Gln Val 
                165                 170                 175     


Ala Gln Val Leu Thr Tyr Ser Met Gly Val Arg Lys Gly Asp Thr Val 
            180                 185                 190         


Ala Val Tyr Met Pro Met Val Pro Glu Ala Ile Ile Thr Leu Leu Ala 
        195                 200                 205             


Ile Ser Arg Ile Gly Ala Ile His Ser Val Val Phe Ala Gly Phe Ser 
    210                 215                 220                 


Ser Asn Ser Leu Arg Asp Arg Ile Asn Asp Gly Asp Ser Lys Val Val 
225                 230                 235                 240 


Ile Thr Thr Asp Glu Ser Asn Arg Gly Gly Lys Val Ile Glu Thr Lys 
                245                 250                 255     


Arg Ile Val Asp Asp Ala Leu Arg Glu Thr Pro Gly Val Arg His Val 
            260                 265                 270         


Leu Val Tyr Arg Lys Thr Asn Asn Pro Ser Val Ala Phe His Ala Pro 
        275                 280                 285             


Arg Asp Leu Asp Trp Ala Thr Glu Lys Lys Lys Tyr Lys Thr Tyr Tyr 
    290                 295                 300                 


Pro Cys Thr Pro Val Asp Ser Glu Asp Pro Leu Phe Leu Leu Tyr Thr 
305                 310                 315                 320 


Ser Gly Ser Thr Gly Ala Pro Lys Gly Val Gln His Ser Thr Ala Gly 
                325                 330                 335     


Tyr Leu Leu Gly Ala Leu Leu Thr Met Arg Tyr Thr Phe Asp Thr His 
            340                 345                 350         


Gln Glu Asp Val Phe Phe Thr Ala Gly Asp Ile Gly Trp Ile Thr Gly 
        355                 360                 365             


His Thr Tyr Val Val Tyr Gly Pro Leu Leu Tyr Gly Cys Ala Thr Leu 
    370                 375                 380                 


Val Phe Glu Gly Thr Pro Ala Tyr Pro Asn Tyr Ser Arg Tyr Trp Asp 
385                 390                 395                 400 


Ile Ile Asp Glu His Lys Val Thr Gln Phe Tyr Val Ala Pro Thr Ala 
                405                 410                 415     


Leu Arg Leu Leu Lys Arg Ala Gly Asp Ser Tyr Ile Glu Asn His Ser 
            420                 425                 430         


Leu Lys Ser Leu Arg Cys Leu Gly Ser Val Gly Glu Pro Ile Ala Ala 
        435                 440                 445             


Glu Val Trp Glu Trp Tyr Ser Glu Lys Ile Gly Lys Asn Glu Ile Pro 
    450                 455                 460                 


Ile Val Asp Thr Tyr Trp Gln Thr Glu Ser Gly Ser His Leu Val Thr 
465                 470                 475                 480 


Pro Leu Ala Gly Gly Val Thr Pro Met Lys Pro Gly Ser Ala Ser Phe 
                485                 490                 495     


Pro Phe Phe Gly Ile Asp Ala Val Val Leu Asp Pro Asn Thr Gly Glu 
            500                 505                 510         


Glu Leu Asn Thr Ser His Ala Glu Gly Val Leu Ala Val Lys Ala Ala 
        515                 520                 525             


Trp Pro Ser Phe Ala Arg Thr Ile Trp Lys Asn His Asp Arg Tyr Leu 
    530                 535                 540                 


Asp Thr Tyr Leu Asn Pro Tyr Pro Gly Tyr Tyr Phe Thr Gly Asp Gly 
545                 550                 555                 560 


Ala Ala Lys Asp Lys Asp Gly Tyr Ile Trp Ile Leu Gly Arg Val Asp 
                565                 570                 575     


Asp Val Val Asn Val Ser Gly His Arg Leu Ser Thr Ala Glu Ile Glu 
            580                 585                 590         


Ala Ala Ile Ile Glu Asp Pro Ile Val Ala Glu Cys Ala Val Val Gly 
        595                 600                 605             


Phe Asn Asp Asp Leu Thr Gly Gln Ala Val Ala Ala Phe Val Val Leu 
    610                 615                 620                 


Lys Asn Lys Ser Ser Trp Ser Thr Ala Thr Asp Asp Glu Leu Gln Asp 
625                 630                 635                 640 


Ile Lys Lys His Leu Val Phe Thr Val Arg Lys Asp Ile Gly Pro Phe 
                645                 650                 655     


Ala Ala Pro Lys Leu Ile Ile Leu Val Asp Asp Leu Pro Lys Thr Arg 
            660                 665                 670         


Ser Gly Lys Ile Met Arg Arg Ile Leu Arg Lys Ile Leu Ala Gly Glu 
        675                 680                 685             


Ser Asp Gln Leu Gly Asp Val Ser Thr Leu Ser Asn Pro Gly Ile Val 
    690                 695                 700                 


Arg His Leu Ile Asp Ser Val Lys Leu 
705                 710             


<210>  20
<211>  683
<212>  PRT
<213>  Saccharomyces cerevisiae ACS2 protein sequence

<400>  20

Met Thr Ile Lys Glu His Lys Val Val Tyr Glu Ala His Asn Val Lys 
1               5                   10                  15      


Ala Leu Lys Ala Pro Gln His Phe Tyr Asn Ser Gln Pro Gly Lys Gly 
            20                  25                  30          


Tyr Val Thr Asp Met Gln His Tyr Gln Glu Met Tyr Gln Gln Ser Ile 
        35                  40                  45              


Asn Glu Pro Glu Lys Phe Phe Asp Lys Met Ala Lys Glu Tyr Leu His 
    50                  55                  60                  


Trp Asp Ala Pro Tyr Thr Lys Val Gln Ser Gly Ser Leu Asn Asn Gly 
65                  70                  75                  80  


Asp Val Ala Trp Phe Leu Asn Gly Lys Leu Asn Ala Ser Tyr Asn Cys 
                85                  90                  95      


Val Asp Arg His Ala Phe Ala Asn Pro Asp Lys Pro Ala Leu Ile Tyr 
            100                 105                 110         


Glu Ala Asp Asp Glu Ser Asp Asn Lys Ile Ile Thr Phe Gly Glu Leu 
        115                 120                 125             


Leu Arg Lys Val Ser Gln Ile Ala Gly Val Leu Lys Ser Trp Gly Val 
    130                 135                 140                 


Lys Lys Gly Asp Thr Val Ala Ile Tyr Leu Pro Met Ile Pro Glu Ala 
145                 150                 155                 160 


Val Ile Ala Met Leu Ala Val Ala Arg Ile Gly Ala Ile His Ser Val 
                165                 170                 175     


Val Phe Ala Gly Phe Ser Ala Gly Ser Leu Lys Asp Arg Val Val Asp 
            180                 185                 190         


Ala Asn Ser Lys Val Val Ile Thr Cys Asp Glu Gly Lys Arg Gly Gly 
        195                 200                 205             


Lys Thr Ile Asn Thr Lys Lys Ile Val Asp Glu Gly Leu Asn Gly Val 
    210                 215                 220                 


Asp Leu Val Ser Arg Ile Leu Val Phe Gln Arg Thr Gly Thr Glu Gly 
225                 230                 235                 240 


Ile Pro Met Lys Ala Gly Arg Asp Tyr Trp Trp His Glu Glu Ala Ala 
                245                 250                 255     


Lys Gln Arg Thr Tyr Leu Pro Pro Val Ser Cys Asp Ala Glu Asp Pro 
            260                 265                 270         


Leu Phe Leu Leu Tyr Thr Ser Gly Ser Thr Gly Ser Pro Lys Gly Val 
        275                 280                 285             


Val His Thr Thr Gly Gly Tyr Leu Leu Gly Ala Ala Leu Thr Thr Arg 
    290                 295                 300                 


Tyr Val Phe Asp Ile His Pro Glu Asp Val Leu Phe Thr Ala Gly Asp 
305                 310                 315                 320 


Val Gly Trp Ile Thr Gly His Thr Tyr Ala Leu Tyr Gly Pro Leu Thr 
                325                 330                 335     


Leu Gly Thr Ala Ser Ile Ile Phe Glu Ser Thr Pro Ala Tyr Pro Asp 
            340                 345                 350         


Tyr Gly Arg Tyr Trp Arg Ile Ile Gln Arg His Lys Ala Thr His Phe 
        355                 360                 365             


Tyr Val Ala Pro Thr Ala Leu Arg Leu Ile Lys Arg Val Gly Glu Ala 
    370                 375                 380                 


Glu Ile Ala Lys Tyr Asp Thr Ser Ser Leu Arg Val Leu Gly Ser Val 
385                 390                 395                 400 


Gly Glu Pro Ile Ser Pro Asp Leu Trp Glu Trp Tyr His Glu Lys Val 
                405                 410                 415     


Gly Asn Lys Asn Cys Val Ile Cys Asp Thr Met Trp Gln Thr Glu Ser 
            420                 425                 430         


Gly Ser His Leu Ile Ala Pro Leu Ala Gly Ala Val Pro Thr Lys Pro 
        435                 440                 445             


Gly Ser Ala Thr Val Pro Phe Phe Gly Ile Asn Ala Cys Ile Ile Asp 
    450                 455                 460                 


Pro Val Thr Gly Val Glu Leu Glu Gly Asn Asp Val Glu Gly Val Leu 
465                 470                 475                 480 


Ala Val Lys Ser Pro Trp Pro Ser Met Ala Arg Ser Val Trp Asn His 
                485                 490                 495     


His Asp Arg Tyr Met Asp Thr Tyr Leu Lys Pro Tyr Pro Gly His Tyr 
            500                 505                 510         


Phe Thr Gly Asp Gly Ala Gly Arg Asp His Asp Gly Tyr Tyr Trp Ile 
        515                 520                 525             


Arg Gly Arg Val Asp Asp Val Val Asn Val Ser Gly His Arg Leu Ser 
    530                 535                 540                 


Thr Ser Glu Ile Glu Ala Ser Ile Ser Asn His Glu Asn Val Ser Glu 
545                 550                 555                 560 


Ala Ala Val Val Gly Ile Pro Asp Glu Leu Thr Gly Gln Thr Val Val 
                565                 570                 575     


Ala Tyr Val Ser Leu Lys Asp Gly Tyr Leu Gln Asn Asn Ala Thr Glu 
            580                 585                 590         


Gly Asp Ala Glu His Ile Thr Pro Asp Asn Leu Arg Arg Glu Leu Ile 
        595                 600                 605             


Leu Gln Val Arg Gly Glu Ile Gly Pro Phe Ala Ser Pro Lys Thr Ile 
    610                 615                 620                 


Ile Leu Val Arg Asp Leu Pro Arg Thr Arg Ser Gly Lys Ile Met Arg 
625                 630                 635                 640 


Arg Val Leu Arg Lys Val Ala Ser Asn Glu Ala Glu Gln Leu Gly Asp 
                645                 650                 655     


Leu Thr Thr Leu Ala Asn Pro Glu Val Val Pro Ala Ile Ile Ser Ala 
            660                 665                 670         


Val Glu Asn Gln Phe Phe Ser Gln Lys Lys Lys 
        675                 680             


<210>  21
<211>  1176
<212>  DNA
<213>  Saccharomyces cerevisiae GPD1 nucleotide sequence

<400>  21
atgtctgctg ctgctgatag attaaactta acttccggcc acttgaatgc tggtagaaag       60

agaagttcct cttctgtttc tttgaaggct gccgaaaagc ctttcaaggt tactgtgatt      120

ggatctggta actggggtac tactattgcc aaggtggttg ccgaaaattg taagggatac      180

ccagaagttt tcgctccaat agtacaaatg tgggtgttcg aagaagagat caatggtgaa      240

aaattgactg aaatcataaa tactagacat caaaacgtga aatacttgcc tggcatcact      300

ctacccgaca atttggttgc taatccagac ttgattgatt cagtcaagga tgtcgacatc      360

atcgttttca acattccaca tcaatttttg ccccgtatct gtagccaatt gaaaggtcat      420

gttgattcac acgtcagagc tatctcctgt ctaaagggtt ttgaagttgg tgctaaaggt      480

gtccaattgc tatcctctta catcactgag gaactaggta ttcaatgtgg tgctctatct      540

ggtgctaaca ttgccaccga agtcgctcaa gaacactggt ctgaaacaac agttgcttac      600

cacattccaa aggatttcag aggcgagggc aaggacgtcg accataaggt tctaaaggcc      660

ttgttccaca gaccttactt ccacgttagt gtcatcgaag atgttgctgg tatctccatc      720

tgtggtgctt tgaagaacgt tgttgcctta ggttgtggtt tcgtcgaagg tctaggctgg      780

ggtaacaacg cttctgctgc catccaaaga gtcggtttgg gtgagatcat cagattcggt      840

caaatgtttt tcccagaatc tagagaagaa acatactacc aagagtctgc tggtgttgct      900

gatttgatca ccacctgcgc tggtggtaga aacgtcaagg ttgctaggct aatggctact      960

tctggtaagg acgcctggga atgtgaaaag gagttgttga atggccaatc cgctcaaggt     1020

ttaattacct gcaaagaagt tcacgaatgg ttggaaacat gtggctctgt cgaagacttc     1080

ccattatttg aagccgtata ccaaatcgtt tacaacaact acccaatgaa gaacctgccg     1140

gacatgattg aagaattaga tctacatgaa gattag                               1176


<210>  22
<211>  1323
<212>  DNA
<213>  Saccharomyces cerevisiae GPD2 (YOL059w) nucleotide sequence

<400>  22
atgcttgctg tcagaagatt aacaagatac acattcctta agcgaacgca tccggtgtta       60

tatactcgtc gtgcatataa aattttgcct tcaagatcta ctttcctaag aagatcatta      120

ttacaaacac aactgcactc aaagatgact gctcatacta atatcaaaca gcacaaacac      180

tgtcatgagg accatcctat cagaagatcg gactctgccg tgtcaattgt acatttgaaa      240

cgtgcgccct tcaaggttac agtgattggt tctggtaact gggggaccac catcgccaaa      300

gtcattgcgg aaaacacaga attgcattcc catatcttcg agccagaggt gagaatgtgg      360

gtttttgatg aaaagatcgg cgacgaaaat ctgacggata tcataaatac aagacaccag      420

aacgttaaat atctacccaa tattgacctg ccccataatc tagtggccga tcctgatctt      480

ttacactcca tcaagggtgc tgacatcctt gttttcaaca tccctcatca atttttacca      540

aacatagtca aacaattgca aggccacgtg gcccctcatg taagggccat ctcgtgtcta      600

aaagggttcg agttgggctc caagggtgtg caattgctat cctcctatgt tactgatgag      660

ttaggaatcc aatgtggcgc actatctggt gcaaacttgg caccggaagt ggccaaggag      720

cattggtccg aaaccaccgt ggcttaccaa ctaccaaagg attatcaagg tgatggcaag      780

gatgtagatc ataagatttt gaaattgctg ttccacagac cttacttcca cgtcaatgtc      840

atcgatgatg ttgctggtat atccattgcc ggtgccttga agaacgtcgt ggcacttgca      900

tgtggtttcg tagaaggtat gggatggggt aacaatgcct ccgcagccat tcaaaggctg      960

ggtttaggtg aaattatcaa gttcggtaga atgtttttcc cagaatccaa agtcgagacc     1020

tactatcaag aatccgctgg tgttgcagat ctgatcacca cctgctcagg cggtagaaac     1080

gtcaaggttg ccacatacat ggccaagacc ggtaagtcag ccttggaagc agaaaaggaa     1140

ttgcttaacg gtcaatccgc ccaagggata atcacatgca gagaagttca cgagtggcta     1200

caaacatgtg agttgaccca agaattccca ttattcgagg cagtctacca gatagtctac     1260

aacaacgtcc gcatggaaga cctaccggag atgattgaag agctagacat cgatgacgaa     1320

tag                                                                   1323


<210>  23
<211>  2142
<212>  DNA
<213>  Saccharomyces cerevisiae ACS1 (YAL054c) nucleotide sequence

<400>  23
atgtcgccct ctgccgtaca atcatcaaaa ctagaagaac agtcaagtga aattgacaag       60

ttgaaagcaa aaatgtccca gtctgccgcc actgcgcagc agaagaagga acatgagtat      120

gaacatttga cttcggtcaa gatcgtgcca caacggccca tctcagatag actgcagccc      180

gcaattgcta cccactattc tccacacttg gacgggttgc aggactatca gcgcttgcac      240

aaggagtcta ttgaagaccc tgctaagttc ttcggttcta aagctaccca atttttaaac      300

tggtctaagc cattcgataa ggtgttcatc ccagacccta aaacgggcag gccctccttc      360

cagaacaatg catggttcct caacggccaa ttaaacgcct gttacaactg tgttgacaga      420

catgccttga agactcctaa caagaaagcc attattttcg aaggtgacga gcctggccaa      480

ggctattcca ttacctacaa ggaactactt gaagaagttt gtcaagtggc acaagtgctg      540

acttactcta tgggcgttcg caagggcgat actgttgccg tgtacatgcc tatggtccca      600

gaagcaatca taaccttgtt ggccatttcc cgtatcggtg ccattcactc cgtagtcttt      660

gccgggtttt cttccaactc cttgagagat cgtatcaacg atggggactc taaagttgtc      720

atcactacag atgaatccaa cagaggtggt aaagtcattg agactaaaag aattgttgat      780

gacgcgctaa gagagacccc aggcgtgaga cacgtcttgg tttatagaaa gaccaacaat      840

ccatctgttg ctttccatgc ccccagagat ttggattggg caacagaaaa gaagaaatac      900

aagacctact atccatgcac acccgttgat tctgaggatc cattattctt gttgtatacg      960

tctggttcta ctggtgcccc caagggtgtt caacattcta ccgcaggtta cttgctggga     1020

gctttgttga ccatgcgcta cacttttgac actcaccaag aagacgtttt cttcacagct     1080

ggagacattg gctggattac aggccacact tatgtggttt atggtccctt actatatggt     1140

tgtgccactt tggtctttga agggactcct gcgtacccaa attactcccg ttattgggat     1200

attattgatg aacacaaagt cacccaattt tatgttgcgc caactgcttt gcgtttgttg     1260

aaaagagctg gtgattccta catcgaaaat cattccttaa aatctttgcg ttgcttgggt     1320

tcggtcggtg agccaattgc tgctgaagtt tgggagtggt actctgaaaa aataggtaaa     1380

aatgaaatcc ccattgtaga cacctactgg caaacagaat ctggttcgca tctggtcacc     1440

ccgctggctg gtggtgttac accaatgaaa ccgggttctg cctcattccc cttcttcggt     1500

attgatgcag ttgttcttga ccctaacact ggtgaagaac ttaacaccag ccacgcagag     1560

ggtgtccttg ccgtcaaagc tgcatggcca tcatttgcaa gaactatttg gaaaaatcat     1620

gataggtatc tagacactta tttgaaccct taccctggct actatttcac tggtgatggt     1680

gctgcaaagg ataaggatgg ttatatctgg attttgggtc gtgtagacga tgtggtgaac     1740

gtctctggtc accgtctgtc taccgctgaa attgaggctg ctattatcga agatccaatt     1800

gtggccgagt gtgctgttgt cggattcaac gatgacttga ctggtcaagc agttgctgca     1860

tttgtggtgt tgaaaaacaa atctagttgg tccaccgcaa cagatgatga attacaagat     1920

atcaagaagc atttggtctt tactgttaga aaagacatcg ggccatttgc cgcaccaaaa     1980

ttgatcattt tagtggatga cttgcccaag acaagatccg gcaaaattat gagacgtatt     2040

ttaagaaaaa tcctagcagg agaaagtgac caactaggcg acgtttctac attgtcaaac     2100

cctggcattg ttagacatct aattgattcg gtcaagttgt aa                        2142


<210>  24
<211>  2052
<212>  DNA
<213>  Saccharomyces cerevisiae ACS2 (YLR153c) nucleotide sequence

<400>  24
atgacaatca aggaacataa agtagtttat gaagctcaca acgtaaaggc tcttaaggct       60

cctcaacatt tttacaacag ccaacccggc aagggttacg ttactgatat gcaacattat      120

caagaaatgt atcaacaatc tatcaatgag ccagaaaaat tctttgataa gatggctaag      180

gaatacttgc attgggatgc tccatacacc aaagttcaat ctggttcatt gaacaatggt      240

gatgttgcat ggtttttgaa cggtaaattg aatgcatcat acaattgtgt tgacagacat      300

gcctttgcta atcccgacaa gccagctttg atctatgaag ctgatgacga atccgacaac      360

aaaatcatca catttggtga attactcaga aaagtttccc aaatcgctgg tgtcttaaaa      420

agctggggcg ttaagaaagg tgacacagtg gctatctatt tgccaatgat tccagaagcg      480

gtcattgcta tgttggctgt ggctcgtatt ggtgctattc actctgttgt ctttgctggg      540

ttctccgctg gttcgttgaa agatcgtgtc gttgacgcta attctaaagt ggtcatcact      600

tgtgatgaag gtaaaagagg tggtaagacc atcaacacta aaaaaattgt tgacgaaggt      660

ttgaacggag tcgatttggt ttcccgtatc ttggttttcc aaagaactgg tactgaaggt      720

attccaatga aggccggtag agattactgg tggcatgagg aggccgctaa gcagagaact      780

tacctacctc ctgtttcatg tgacgctgaa gatcctctat ttttattata cacttccggt      840

tccactggtt ctccaaaggg tgtcgttcac actacaggtg gttatttatt aggtgccgct      900

ttaacaacta gatacgtttt tgatattcac ccagaagatg ttctcttcac tgccggtgac      960

gtcggctgga tcacgggtca cacctatgct ctatatggtc cattaacctt gggtaccgcc     1020

tcaataattt tcgaatccac tcctgcctac ccagattatg gtagatattg gagaattatc     1080

caacgtcaca aggctaccca tttctatgtg gctccaactg ctttaagatt aatcaaacgt     1140

gtaggtgaag ccgaaattgc caaatatgac acttcctcat tacgtgtctt gggttccgtc     1200

ggtgaaccaa tctctccaga cttatgggaa tggtatcatg aaaaagtggg taacaaaaac     1260

tgtgtcattt gtgacactat gtggcaaaca gagtctggtt ctcatttaat tgctcctttg     1320

gcaggtgctg tcccaacaaa acctggttct gctaccgtgc cattctttgg tattaacgct     1380

tgtatcattg accctgttac aggtgtggaa ttagaaggta atgatgtcga aggtgtcctt     1440

gccgttaaat caccatggcc atcaatggct agatctgttt ggaaccacca cgaccgttac     1500

atggatactt acttgaaacc ttatcctggt cactatttca caggtgatgg tgctggtaga     1560

gatcatgatg gttactactg gatcaggggt agagttgacg acgttgtaaa tgtttccggt     1620

catagattat ccacatcaga aattgaagca tctatctcaa atcacgaaaa cgtctcggaa     1680

gctgctgttg tcggtattcc agatgaattg accggtcaaa ccgtcgttgc atatgtttcc     1740

ctaaaagatg gttatctaca aaacaacgct actgaaggtg atgcagaaca catcacacca     1800

gataatttac gtagagaatt gatcttacaa gttaggggtg agattggtcc tttcgcctca     1860

ccaaaaacca ttattctagt tagagatcta ccaagaacaa ggtcaggaaa gattatgaga     1920

agagttctaa gaaaggttgc ttctaacgaa gccgaacagc taggtgacct aactactttg     1980

gccaacccag aagttgtacc tgccatcatt tctgctgtag agaaccaatt tttctctcaa     2040

aaaaagaaat aa                                                         2052


