                         SEQUENCE LISTING

<110>  Tufts University
 
<120>  HOMOPOLYMER MEDIATED NUCLEIC ACID AMPLIFICATION

<130>  T0605.70016WO00

<140>  N/A
<141>  2013-08-23

<150>  61/692,502
<151>  2012-08-23

<160>  100   

<170>  PatentIn version 3.5

<210>  1
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  1
gatcggaaga gctcgtatgc cgtcttctgc ttg                                    33


<210>  2
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  2
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  3
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  3
caagcagaag acggcatacg agctcttccg atct                                   34


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  4
acactctttc cctacacgac gctcttccga tct                                    33


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  5
gatcggaaga gcacacgtct                                                   20


<210>  6
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  6
acactctttc cctacacgac gctcttccga tct                                    33


<210>  7
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  7
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  8
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  8
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  9
acactctttc cctacacgac gctcttccga tct                                    33


<210>  10
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  10
gatcggaaga gcacacgtct gaactccagt cac                                    33


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  11
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  12
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  12
caagcagaag acggcatacg agatcgtgat gtgactggag ttc                         43


<210>  13
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  13
caagcagaag acggcatacg agatacatcg gtgactggag ttc                         43


<210>  14
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  14
caagcagaag acggcatacg agatgcctaa gtgactggag ttc                         43


<210>  15
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  15
caagcagaag acggcatacg agattggtca gtgactggag ttc                         43


<210>  16
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  16
caagcagaag acggcatacg agatcactgt gtgactggag ttc                         43


<210>  17
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  17
caagcagaag acggcatacg agatattggc gtgactggag ttc                         43


<210>  18
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  18
caagcagaag acggcatacg agatgatctg gtgactggag ttc                         43


<210>  19
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  19
caagcagaag acggcatacg agattcaagt gtgactggag ttc                         43


<210>  20
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  20
caagcagaag acggcatacg agatctgatc gtgactggag ttc                         43


<210>  21
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  21
caagcagaag acggcatacg agataagcta gtgactggag ttc                         43


<210>  22
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  22
caagcagaag acggcatacg agatgtagcc gtgactggag ttc                         43


<210>  23
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  23
caagcagaag acggcatacg agattacaag gtgactggag ttc                         43


<210>  24
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  24
gatcggaaga gcggttcagc aggaatgccg ag                                     32


<210>  25
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  25
acactctttc cctacacgac gctcttccga tct                                    33


<210>  26
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  26
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  27
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  27
gatcgtcgga ctgtagaact ctgaac                                            26


<210>  28
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  28
acaggttcag agttctacag tccgac                                            26


<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, or t

<400>  29
caagcagaag acggcatacg ann                                               23


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  30
tcgtatgccg tcttctgctt g                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  31
caagcagaag acggcatacg a                                                 21


<210>  32
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  32
aatgatacgg cgaccaccga caggttcaga gttctacagt ccga                        44


<210>  33
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  33
cgacaggttc agagttctac agtccgacga tc                                     32


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  34
tcggactgta gaactctgaa c                                                 21


<210>  35
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  35
acaggttcag agttctacag tccgacatg                                         29


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, or t

<400>  36
caagcagaag acggcatacg ann                                               23


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  37
tcgtatgccg tcttctgctt g                                                 21


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  38
caagcagaag acggcatacg a                                                 21


<210>  39
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  39
aatgatacgg cgaccaccga caggttcaga gttctacagt ccga                        44


<210>  40
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  40
ccgacaggtt cagagttcta cagtccgaca tg                                     32


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  41
caagcagaag acggcatacg a                                                 21


<210>  42
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  42
guucagaguu cuacaguccg acgauc                                            26


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is inverted dT or ddT

<400>  43
ucguaugccg ucuucugcuu gun                                              23


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  44
caagcagaag acggcatacg a                                                 21


<210>  45
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  45
aatgatacgg cgaccaccga caggttcaga gttctacagt ccga                        44


<210>  46
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  46
cgacaggttc agagttctac agtccgacga tc                                     32


<210>  47
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  47
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctcg gggggg           56


<210>  48
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  48
acactctttc cctacacgac gctcgggggg g                                      31


<210>  49
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  49
caagcagaag acggcatacg agataaaaaa gtgactggag ttcagacgtg tgctcttccg       60

atctgggggg gggggggggg                                                   80


<210>  50
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  50
caagcagaag acggcatacg agatacacac gtgactggag ttcagacgtg tgctcttccg       60

atctgggggg gggggggggg                                                   80


<210>  51
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  51
caagcagaag acggcatacg agatagagag gtgactggag ttcagacgtg tgctcttccg       60

atctgggggg gggggggggg                                                   80


<210>  52
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  52
caagcagaag acggcatacg agatatatat gtgactggag ttcagacgtg tgctcttccg       60

atctgggggg gggggggggg                                                   80


<210>  53
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  53
caagcagaag acggcatacg agatcacaca gtgactggag ttcagacgtg tgctcttccg       60

atctgggggg gggggggggg                                                   80


<210>  54
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  54
ccaaaatccg ttcctttttc atagttccta tatagttata cgc                         43


<210>  55
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  55
gtgactggag ttcagacgtg tgctcttccg atctgggggg gggggggggg                  50


<210>  56
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  56
aatgatacgg cgaccaccga gatctacact ctttgaccgg ggacttatca gccaacctgt       60

ta                                                                      62


<210>  57
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  57
acactctttg accggggact tatcagccaa cctgtta                                37


<210>  58
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  58
caagcagaag acggcatacg agatcgtgat gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  59
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  59
caagcagaag acggcatacg agatacatcg gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  60
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  60
caagcagaag acggcatacg agatgcctaa gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  61
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  61
caagcagaag acggcatacg agattggtca gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  62
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  62
caagcagaag acggcatacg agatcactgt gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  63
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  63
caagcagaag acggcatacg agatattggc gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  64
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  64
caagcagaag acggcatacg agatgatctg gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  65
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  65
caagcagaag acggcatacg agattcaagt gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  66
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  66
caagcagaag acggcatacg agatctgatc gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  67
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  67
caagcagaag acggcatacg agataagcta gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  68
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  68
caagcagaag acggcatacg agatgtagcc gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  69
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  69
caagcagaag acggcatacg agattacaag gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  70
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  70
caagcagaag acggcatacg agattgttga ctgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  71
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  71
caagcagaag acggcatacg agatacggaa ctgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  72
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  72
caagcagaag acggcatacg agattctgac atgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  73
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  73
caagcagaag acggcatacg agatcgggac gggtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  74
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  74
caagcagaag acggcatacg agatgtgcgg acgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  75
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  75
caagcagaag acggcatacg agatcgtttc acgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  76
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  76
caagcagaag acggcatacg agataaggcc acgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  77
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  77
caagcagaag acggcatacg agataccgaa acgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  78
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  78
caagcagaag acggcatacg agattacgta cggtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  79
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  79
caagcagaag acggcatacg agatatccac tcgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  80
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  80
caagcagaag acggcatacg agatatatca gtgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  81
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  81
caagcagaag acggcatacg agataaagga atgtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  82
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  82
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctcg gggggg           56


<210>  83
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  83
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctcg ggttttttt        59


<210>  84
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (25)..(30)
<223>  n is a, c, g, or t, reverse complement of the barcode

<400>  84
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tgctcttccg       60

atctgggggg gggggggggg                                                   80


<210>  85
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  85
caagcagaag acggcatacg agatcgtgat gtgactggag ttcagacgtg tgctcttccg       60

atcttttttt tttttttttt ttttt                                             85


<210>  86
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  86
acactctttc cctacacgac gctcgggggg g                                      31


<210>  87
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  87
gtgactggag ttcagacgtg tgctcttccg atctgggggg gggggggggg                  50


<210>  88
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  88
cctgacggat ggcctttttg cgtttctacc                                        30


<210>  89
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  89
aatgatacgg cgaccaccga gatctacact ctttgaccgg ggacttatca tccaacctgt       60

ta                                                                      62


<210>  90
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (25)..(30)
<223>  n is a, c, g, or t, reverse complement of the index

<400>  90
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  91
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  91
acactctttg accggggact tatcatccaa cctgtta                                37


<210>  92
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  92
cccccccccc cccccccccc cccccc                                            26


<210>  93
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  93
gatc                                                                     4


<210>  94
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  94
ccggc                                                                    5


<210>  95
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  95
ccggt                                                                    5


<210>  96
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  96
cggcaagttc atccttagga gaccgggg                                          28


<210>  97
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  97
gtgactggag ttcagacgtg tgctcttccg atctgggggg gggggggggg                  50


<210>  98
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  98
aatgatacgg cgaccaccga gatctacact ctttccgggg acttatcagc caacctgtta       60


<210>  99
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (25)..(30)
<223>  n is a, c, g, or t, six-base-pair barcode sequence

<400>  99
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  100
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  100
acactctttc cggggactta tcagccaacc tgtta                                  35