                                SEQUENCE LISTING

<110> The Government of the United States of America
   as represented by The Secretary of the
   Department of Health and Human Services

<120> Engineered Anthrax Lethal Toxin for
  Targeted Delivery

<130> 77867-884174

<140> WO Not yet assigned    
<141> Not yet assigned  

<150> US 61/692,143       
<151> 2012-08-22  

<160> 17

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer used for site-directed mutagenesis
      reversion of D512K point mutation to wild type

<400> 1
ggatagcggc ggttaatcct agtgatccat tagaaacgac taa                   43

<210> 2
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic sequence used construction of PA-U2-D512K
      with pYS5-PA-U2

<400> 2
ggatagcggc ggttaatcct agtaagccat tagaaacgac taa                   43

<210> 3
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic sequence used construction of PA-L1-GN
      with pYS5-PA-L1

<400> 3
ggttacagga cggattgatg gaaatgtatc accagaggca aaccaccccc ttg        53

<210> 4
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic sequence used construction of PA-L1-NS
      with pYS5-PA-L1

<400> 4
ggttacagga cggattgata acaatgtatc accagaggca agccaccccc ttg        53

<210> 5
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic overlap extension PCR forward inner primer

<400> 5
cttctgatcc gtacagtgat ttcgaarrmg ttacaggarr mattgatrrm aatgtatcac 60
cagaggcarr mcaccccctt gtggcagc                                    88

<210> 6
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic overlap extension PCR reverse inner primer

<400> 6
ttcgaaatca ctgtacggat cagaag                                      26

<210> 7
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primary and secondary amplification
      forward outer primer

<400> 7
gacgagcgct tcggtcttaa ctg                                         23

<210> 8
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primary and secondary amplification
      reverse outer primer

<400> 8
agcagccaac tcagcttcct ttcg                                        24

<210> 9
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic matrix metalloproteinase (MMP)
      recognized cleavage site

<400> 9
Gly Pro Leu Gly Met Leu Ser Gln
 1               5              


<210> 10
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic matrix metalloproteinase (MMP)
      recognized cleavage site

<400> 10
Gly Pro Leu Gly Leu Trp Ala Gln
 1               5              


<210> 11
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic plasminogen activator recognized
      cleavage site

<400> 11
Pro Cys Pro Gly Arg Val Val Gly Gly
 1               5                  


<210> 12
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic plasminogen activator recognized
      cleavage site

<400> 12
Pro Gly Ser Gly Arg Ser Ala
 1               5          


<210> 13
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic plasminogen activator recognized
      cleavage site

<400> 13
Pro Gly Ser Gly Lys Ser Ala
 1               5          


<210> 14
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic plasminogen activator recognized
      cleavage site

<400> 14
Pro Gln Arg Gly Arg Ser Ala
 1               5          


<210> 15
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic native protective antigen (PA)
      furin-recognized cleavage site

<400> 15
Arg Lys Lys Arg
 1              


<210> 16
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic protective antigen (PA) furin-recognized
      cleavage site replacement mutant

<400> 16
Arg Ala Ala Arg
 1              


<210> 17
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic protective antigen (PA) furin-recognized
      cleavage site replacement mutant

<400> 17
Arg Ala Arg Ala Ala Arg
 1               5      

