                         SEQUENCE LISTING

<110>  Gjermansen, Morten
 
<120>  New metalloprotease from exiguobacterium

<130>  12406

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  1733
<212>  DNA
<213>  Exiguobacterium oxidotolerans


<220>
<221>  CDS
<222>  (101)..(1633)

<220>
<221>  sig_peptide
<222>  (101)..(166)

<220>
<221>  mat_peptide
<222>  (683)..(1633)

<400>  1
acctattttc agaaaattct atttaatccg aatgccttat tctcgttctc tattttgttt       60

ggcatagtta gactcaaccc aaacaaggag gcgatttatt ttg aaa aag ttt ctc        115
                                            Met Lys Lys Phe Leu           
                                                            -190          

gct aca tcg ctt gtc  gca agt gta ctt gtc  gtt cct aca gtc gta          160
Ala Thr Ser Leu Val  Ala Ser Val Leu Val  Val Pro Thr Val Val             
                -185                 -180                 -175            

ggc gca gaa ggt ctt  caa tct ggt aag ttg  acg aaa gca tcg tcc          205
Gly Ala Glu Gly Leu  Gln Ser Gly Lys Leu  Thr Lys Ala Ser Ser             
                -170                 -165                 -160            

gag cca gct gca tca  atc gtt aaa gag tat  gtc gaa ggt aaa ggt          250
Glu Pro Ala Ala Ser  Ile Val Lys Glu Tyr  Val Glu Gly Lys Gly             
                -155                 -150                 -145            

gac ttc tct gta caa  gat gtt caa aaa gat  gga tca tct cac atc          295
Asp Phe Ser Val Gln  Asp Val Gln Lys Asp  Gly Ser Ser His Ile             
                -140                 -135                 -130            

gta cgt ctc caa caa  gaa gtg gat ggc gtc  ccg gtc ttc ggt agt          340
Val Arg Leu Gln Gln  Glu Val Asp Gly Val  Pro Val Phe Gly Ser             
                -125                 -120                 -115            

gtc gtc gtc ggt aac  gtt gcg aaa gac ggt  act tta aaa gca atc          385
Val Val Val Gly Asn  Val Ala Lys Asp Gly  Thr Leu Lys Ala Ile             
                -110                 -105                 -100            

gtc aat gat gca gtc aag gtc aaa ggg aaa cca ggt ctt gat aag aaa        433
Val Asn Asp Ala Val Lys Val Lys Gly Lys Pro Gly Leu Asp Lys Lys           
                -95                 -90                 -85               

att gca atc tct gaa aag aaa gct tta aag ttg tat aaa aaa tca atc        481
Ile Ala Ile Ser Glu Lys Lys Ala Leu Lys Leu Tyr Lys Lys Ser Ile           
            -80                 -75                 -70                   

aaa gca aca gaa ttc gaa gtg gct cca acg agt gaa ctc gtt gtc tat        529
Lys Ala Thr Glu Phe Glu Val Ala Pro Thr Ser Glu Leu Val Val Tyr           
        -65                 -60                 -55                       

cca gtc aaa gac gat gct atc tat gct tat aaa atc aca tcg act gtc        577
Pro Val Lys Asp Asp Ala Ile Tyr Ala Tyr Lys Ile Thr Ser Thr Val           
    -50                 -45                 -40                           

tta gct ggc gac gag ccg tca cgc tgg aca tac ttc atc gat gca aac        625
Leu Ala Gly Asp Glu Pro Ser Arg Trp Thr Tyr Phe Ile Asp Ala Asn           
-35                 -30                 -25                 -20           

tct ggt aag gtc atc aac aaa tat gac caa ctg gca cat gcg aaa cca        673
Ser Gly Lys Val Ile Asn Lys Tyr Asp Gln Leu Ala His Ala Lys Pro           
                -15                 -10                 -5                

gtt acg aca atc acc gga aca acg aca gtc ggt tca ggt acg acg gtt        721
Val Thr Thr Ile Thr Gly Thr Thr Thr Val Gly Ser Gly Thr Thr Val           
        -1  1               5                   10                        

ctt gga aca agt gct aca ttc aac aca acg aaa aat ggt tct tac tac        769
Leu Gly Thr Ser Ala Thr Phe Asn Thr Thr Lys Asn Gly Ser Tyr Tyr           
    15                  20                  25                            

tac ctt caa gat tca aca cgc ggt aaa ggc atc tcg acg tac gat gca        817
Tyr Leu Gln Asp Ser Thr Arg Gly Lys Gly Ile Ser Thr Tyr Asp Ala           
30                  35                  40                  45            

aaa aac cgg aca tca ctt cca gga tca ctt tgg gcg gat gcc gac aac        865
Lys Asn Arg Thr Ser Leu Pro Gly Ser Leu Trp Ala Asp Ala Asp Asn           
                50                  55                  60                

gtt ctc aat gct act tat gac cgt gcc gct gtc agc gca caa gtc aat        913
Val Leu Asn Ala Thr Tyr Asp Arg Ala Ala Val Ser Ala Gln Val Asn           
            65                  70                  75                    

gca gtg aaa aca tat gac ttc tac aaa aac aca tat ggc cgc aac agt        961
Ala Val Lys Thr Tyr Asp Phe Tyr Lys Asn Thr Tyr Gly Arg Asn Ser           
        80                  85                  90                        

tac gat aat gcg ggt gca gca ctc aat tca acg gtt cac tat tct tca       1009
Tyr Asp Asn Ala Gly Ala Ala Leu Asn Ser Thr Val His Tyr Ser Ser           
    95                  100                 105                           

cgc tac aac aat gcg ttc tgg gat gga tca aaa atg gtc tac ggt gac       1057
Arg Tyr Asn Asn Ala Phe Trp Asp Gly Ser Lys Met Val Tyr Gly Asp           
110                 115                 120                 125           

gga gac ggt tcg aca ttc aca tat ctt tct ggc gca ctt gat gta gtc       1105
Gly Asp Gly Ser Thr Phe Thr Tyr Leu Ser Gly Ala Leu Asp Val Val           
                130                 135                 140               

gct cac gaa ttg acg cac gct gtc act gaa tac aca gca gga ctc gtt       1153
Ala His Glu Leu Thr His Ala Val Thr Glu Tyr Thr Ala Gly Leu Val           
            145                 150                 155                   

tac caa aac gaa tct ggt gcg atc aat gag gct gtt tcg gat atc atg       1201
Tyr Gln Asn Glu Ser Gly Ala Ile Asn Glu Ala Val Ser Asp Ile Met           
        160                 165                 170                       

gga aca gtt gca gag tac tca gtc ggc tca aac ttc gac tgg ctc gtc       1249
Gly Thr Val Ala Glu Tyr Ser Val Gly Ser Asn Phe Asp Trp Leu Val           
    175                 180                 185                           

ggt gaa gac atc tac aca cca ggt gta agt ggc gat gct ctt cgt tca       1297
Gly Glu Asp Ile Tyr Thr Pro Gly Val Ser Gly Asp Ala Leu Arg Ser           
190                 195                 200                 205           

atg tct aac cct gcc gct tac gga gat cca gac cac tac tca aaa cgt       1345
Met Ser Asn Pro Ala Ala Tyr Gly Asp Pro Asp His Tyr Ser Lys Arg           
                210                 215                 220               

tac aca ggt acg gct gat aac ggt ggc gtc cac acg aac tct ggt atc       1393
Tyr Thr Gly Thr Ala Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile           
            225                 230                 235                   

gtc aac aaa gcc gct tac ctc tta ggt aac ggt ggt aca cat tac ggt       1441
Val Asn Lys Ala Ala Tyr Leu Leu Gly Asn Gly Gly Thr His Tyr Gly           
        240                 245                 250                       

gtc act gta cca ggc gtc ggc gta ccg aaa ctt ggt gcc atc tac tac       1489
Val Thr Val Pro Gly Val Gly Val Pro Lys Leu Gly Ala Ile Tyr Tyr           
    255                 260                 265                           

cgt gcc ctt aac gtc tac ttg act ccg act tcg aac ttc agc tac ctc       1537
Arg Ala Leu Asn Val Tyr Leu Thr Pro Thr Ser Asn Phe Ser Tyr Leu           
270                 275                 280                 285           

cgc gca gca gtc gtt caa tcg gca aaa gac ctt tac ggt tct tca agt       1585
Arg Ala Ala Val Val Gln Ser Ala Lys Asp Leu Tyr Gly Ser Ser Ser           
                290                 295                 300               

gct gaa gca gcc gct gca gca aaa tca ttt gat gca gtt ggc gtc tac       1633
Ala Glu Ala Ala Ala Ala Ala Lys Ser Phe Asp Ala Val Gly Val Tyr           
            305                 310                 315                   

taagaaaacc tcctcccaag gattttctct ttcctctcga gtcgatgact cgggaggttt     1693

tttattgtgg atccaacgca aaaaagcacg ttcacttgac                           1733


<210>  2
<211>  511
<212>  PRT
<213>  Exiguobacterium oxidotolerans

<400>  2

Met Lys Lys Phe Leu  Ala Thr Ser Leu Val  Ala Ser Val Leu Val  
                -190                 -185                 -180 


Val Pro Thr Val Val  Gly Ala Glu Gly Leu  Gln Ser Gly Lys Leu  
                -175                 -170                 -165 


Thr Lys Ala Ser Ser  Glu Pro Ala Ala Ser  Ile Val Lys Glu Tyr  
                -160                 -155                 -150 


Val Glu Gly Lys Gly  Asp Phe Ser Val Gln  Asp Val Gln Lys Asp  
                -145                 -140                 -135 


Gly Ser Ser His Ile  Val Arg Leu Gln Gln  Glu Val Asp Gly Val  
                -130                 -125                 -120 


Pro Val Phe Gly Ser  Val Val Val Gly Asn  Val Ala Lys Asp Gly  
                -115                 -110                 -105 


Thr Leu Lys Ala Ile  Val Asn Asp Ala Val Lys Val Lys Gly Lys Pro 
                -100                 -95                 -90     


Gly Leu Asp Lys Lys Ile Ala Ile Ser Glu Lys Lys Ala Leu Lys Leu 
            -85                 -80                 -75         


Tyr Lys Lys Ser Ile Lys Ala Thr Glu Phe Glu Val Ala Pro Thr Ser 
        -70                 -65                 -60             


Glu Leu Val Val Tyr Pro Val Lys Asp Asp Ala Ile Tyr Ala Tyr Lys 
    -55                 -50                 -45                 


Ile Thr Ser Thr Val Leu Ala Gly Asp Glu Pro Ser Arg Trp Thr Tyr 
-40                 -35                 -30                 -25 


Phe Ile Asp Ala Asn Ser Gly Lys Val Ile Asn Lys Tyr Asp Gln Leu 
                -20                 -15                 -10     


Ala His Ala Lys Pro Val Thr Thr Ile Thr Gly Thr Thr Thr Val Gly 
            -5              -1  1               5               


Ser Gly Thr Thr Val Leu Gly Thr Ser Ala Thr Phe Asn Thr Thr Lys 
    10                  15                  20                  


Asn Gly Ser Tyr Tyr Tyr Leu Gln Asp Ser Thr Arg Gly Lys Gly Ile 
25                  30                  35                  40  


Ser Thr Tyr Asp Ala Lys Asn Arg Thr Ser Leu Pro Gly Ser Leu Trp 
                45                  50                  55      


Ala Asp Ala Asp Asn Val Leu Asn Ala Thr Tyr Asp Arg Ala Ala Val 
            60                  65                  70          


Ser Ala Gln Val Asn Ala Val Lys Thr Tyr Asp Phe Tyr Lys Asn Thr 
        75                  80                  85              


Tyr Gly Arg Asn Ser Tyr Asp Asn Ala Gly Ala Ala Leu Asn Ser Thr 
    90                  95                  100                 


Val His Tyr Ser Ser Arg Tyr Asn Asn Ala Phe Trp Asp Gly Ser Lys 
105                 110                 115                 120 


Met Val Tyr Gly Asp Gly Asp Gly Ser Thr Phe Thr Tyr Leu Ser Gly 
                125                 130                 135     


Ala Leu Asp Val Val Ala His Glu Leu Thr His Ala Val Thr Glu Tyr 
            140                 145                 150         


Thr Ala Gly Leu Val Tyr Gln Asn Glu Ser Gly Ala Ile Asn Glu Ala 
        155                 160                 165             


Val Ser Asp Ile Met Gly Thr Val Ala Glu Tyr Ser Val Gly Ser Asn 
    170                 175                 180                 


Phe Asp Trp Leu Val Gly Glu Asp Ile Tyr Thr Pro Gly Val Ser Gly 
185                 190                 195                 200 


Asp Ala Leu Arg Ser Met Ser Asn Pro Ala Ala Tyr Gly Asp Pro Asp 
                205                 210                 215     


His Tyr Ser Lys Arg Tyr Thr Gly Thr Ala Asp Asn Gly Gly Val His 
            220                 225                 230         


Thr Asn Ser Gly Ile Val Asn Lys Ala Ala Tyr Leu Leu Gly Asn Gly 
        235                 240                 245             


Gly Thr His Tyr Gly Val Thr Val Pro Gly Val Gly Val Pro Lys Leu 
    250                 255                 260                 


Gly Ala Ile Tyr Tyr Arg Ala Leu Asn Val Tyr Leu Thr Pro Thr Ser 
265                 270                 275                 280 


Asn Phe Ser Tyr Leu Arg Ala Ala Val Val Gln Ser Ala Lys Asp Leu 
                285                 290                 295     


Tyr Gly Ser Ser Ser Ala Glu Ala Ala Ala Ala Ala Lys Ser Phe Asp 
            300                 305                 310         


Ala Val Gly Val Tyr 
        315         


<210>  3
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  3
gttcatcgat cgcatcggct gaaggtcttc aatctggtaa g                           41


<210>  4
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  4
gcgttttttt attgattaac gcgtttagta gacgccaact gc                          42


<210>  5
<211>  317
<212>  PRT
<213>  Exiguobacterium oxidotolerans

<400>  5

Ile Thr Gly Thr Thr Thr Val Gly Ser Gly Thr Thr Val Leu Gly Thr 
1               5                   10                  15      


Ser Ala Thr Phe Asn Thr Thr Lys Asn Gly Ser Tyr Tyr Tyr Leu Gln 
            20                  25                  30          


Asp Ser Thr Arg Gly Lys Gly Ile Ser Thr Tyr Asp Ala Lys Asn Arg 
        35                  40                  45              


Thr Ser Leu Pro Gly Ser Leu Trp Ala Asp Ala Asp Asn Val Leu Asn 
    50                  55                  60                  


Ala Thr Tyr Asp Arg Ala Ala Val Ser Ala Gln Val Asn Ala Val Lys 
65                  70                  75                  80  


Thr Tyr Asp Phe Tyr Lys Asn Thr Tyr Gly Arg Asn Ser Tyr Asp Asn 
                85                  90                  95      


Ala Gly Ala Ala Leu Asn Ser Thr Val His Tyr Ser Ser Arg Tyr Asn 
            100                 105                 110         


Asn Ala Phe Trp Asp Gly Ser Lys Met Val Tyr Gly Asp Gly Asp Gly 
        115                 120                 125             


Ser Thr Phe Thr Tyr Leu Ser Gly Ala Leu Asp Val Val Ala His Glu 
    130                 135                 140                 


Leu Thr His Ala Val Thr Glu Tyr Thr Ala Gly Leu Val Tyr Gln Asn 
145                 150                 155                 160 


Glu Ser Gly Ala Ile Asn Glu Ala Val Ser Asp Ile Met Gly Thr Val 
                165                 170                 175     


Ala Glu Tyr Ser Val Gly Ser Asn Phe Asp Trp Leu Val Gly Glu Asp 
            180                 185                 190         


Ile Tyr Thr Pro Gly Val Ser Gly Asp Ala Leu Arg Ser Met Ser Asn 
        195                 200                 205             


Pro Ala Ala Tyr Gly Asp Pro Asp His Tyr Ser Lys Arg Tyr Thr Gly 
    210                 215                 220                 


Thr Ala Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile Val Asn Lys 
225                 230                 235                 240 


Ala Ala Tyr Leu Leu Gly Asn Gly Gly Thr His Tyr Gly Val Thr Val 
                245                 250                 255     


Pro Gly Val Gly Val Pro Lys Leu Gly Ala Ile Tyr Tyr Arg Ala Leu 
            260                 265                 270         


Asn Val Tyr Leu Thr Pro Thr Ser Asn Phe Ser Tyr Leu Arg Ala Ala 
        275                 280                 285             


Val Val Gln Ser Ala Lys Asp Leu Tyr Gly Ser Ser Ser Ala Glu Ala 
    290                 295                 300                 


Ala Ala Ala Ala Lys Ser Phe Asp Ala Val Gly Val Tyr 
305                 310                 315         


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal

<400>  6

Ile Thr Gly Thr Thr Thr Val Gly Ser 
1               5                   


<210>  7
<211>  300
<212>  PRT
<213>  Bacillus amyloliquefaciens

<400>  7

Ala Ala Thr Thr Gly Thr Gly Thr Thr Leu Lys Gly Lys Thr Val Ser 
1               5                   10                  15      


Leu Asn Ile Ser Ser Glu Ser Gly Lys Tyr Val Leu Arg Asp Leu Ser 
            20                  25                  30          


Lys Pro Thr Gly Thr Gln Ile Ile Thr Tyr Asp Leu Gln Asn Arg Glu 
        35                  40                  45              


Tyr Asn Leu Pro Gly Thr Leu Val Ser Ser Thr Thr Asn Gln Phe Thr 
    50                  55                  60                  


Thr Ser Ser Gln Arg Ala Ala Val Asp Ala His Tyr Asn Leu Gly Lys 
65                  70                  75                  80  


Val Tyr Asp Tyr Phe Tyr Gln Lys Phe Asn Arg Asn Ser Tyr Asp Asn 
                85                  90                  95      


Lys Gly Gly Lys Ile Val Ser Ser Val His Tyr Gly Ser Arg Tyr Asn 
            100                 105                 110         


Asn Ala Ala Trp Ile Gly Asp Gln Met Ile Tyr Gly Asp Gly Asp Gly 
        115                 120                 125             


Ser Phe Phe Ser Pro Leu Ser Gly Ser Met Asp Val Thr Ala His Glu 
    130                 135                 140                 


Met Thr His Gly Val Thr Gln Glu Thr Ala Asn Leu Asn Tyr Glu Asn 
145                 150                 155                 160 


Gln Pro Gly Ala Leu Asn Glu Ser Phe Ser Asp Val Phe Gly Tyr Phe 
                165                 170                 175     


Asn Asp Thr Glu Asp Trp Asp Ile Gly Glu Asp Ile Thr Val Ser Gln 
            180                 185                 190         


Pro Ala Leu Arg Ser Leu Ser Asn Pro Thr Lys Tyr Gly Gln Pro Asp 
        195                 200                 205             


Asn Phe Lys Asn Tyr Lys Asn Leu Pro Asn Thr Asp Ala Gly Asp Tyr 
    210                 215                 220                 


Gly Gly Val His Thr Asn Ser Gly Ile Pro Asn Lys Ala Ala Tyr Asn 
225                 230                 235                 240 


Thr Ile Thr Lys Ile Gly Val Asn Lys Ala Glu Gln Ile Tyr Tyr Arg 
                245                 250                 255     


Ala Leu Thr Val Tyr Leu Thr Pro Ser Ser Thr Phe Lys Asp Ala Lys 
            260                 265                 270         


Ala Ala Leu Ile Gln Ser Ala Arg Asp Leu Tyr Gly Ser Gln Asp Ala 
        275                 280                 285             


Ala Ser Val Glu Ala Ala Trp Asn Ala Val Gly Leu 
    290                 295                 300 


