                         SEQUENCE LISTING

<110>  The University of Chicago
       Alverdy, John C
 
<120>  Methods of preventing and treating anastomotic leaks.

<130>  27373/46776A

<150>  U.S. 61/684,641
<151>  2012-08-17

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  916
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(916)
<223>  mexTP1 open reading frame

<400>  1
catgaaccga aacgacctgc gccgcgtcga tctgaacctg ctgatcgtgt tcgagaccct       60

gatgcacgaa cgcagcgtga cccgcgccgc agagaaactg ttcctcggcc agccggccat      120

cagcgccgcg ctgtcgcgcc tgcgcacgct gttcgacgac ccgctgttcg tccgtaccgg      180

acgcagcatg gagcccaccg cgcgagccca ggaaatcttc gcccacctgt cgccggcgct      240

ggattccatc tccaccgcca tgagtcgcgc cagcgagttc gatccggcga ccagcaccgc      300

ggtgttccgc atcggccttt ccgacgacgt cgagttcggc ctgttgccgc ccctgctccg      360

ccgcctgcgc gcggaggcgc cggggatcgt cctcgtcgtg cgccgcgcca actatctatt      420

gatgccgaac ctgctggcct cgggggagat ctcggtgggc gtcagctaca ccgacgaact      480

gccggccaac gccaagcgca agaccgtgcg ccgcagcaag ccgaagatcc tccgcgccga      540

ctccgcgccc ggccagctga ccctcgacga ctattgcgcg cgaccgcacg cgctggtgtc      600

cttcgccggc gacctcagcg gcttcgtcga cgaggagctg gaaaaattcg gccgcaagcg      660

caaggtggtc ctggcggtgc cgcagttcaa cggcctcggc accctcctgg ccggcaccga      720

catcatcgcc accgtgcccg actacgccgc ccaggcgctg atcgccgccg gcggcctacg      780

cgccgaggac ccaccgttcg agacccgggc cttcgaactg tcgatggctt ggcgcggcgc      840

ccaggacaac gatccggccg aacgctggct gcgctcgcgg atcagcatgt tcatcggcga      900

tccggacagt ctctga                                                      916


<210>  2
<211>  304
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(304)
<223>  MexTP1 protein sequence

<400>  2

Met Asn Arg Asn Asp Leu Arg Arg Val Asp Leu Asn Leu Leu Ile Val 
1               5                   10                  15      


Phe Glu Thr Leu Met His Glu Arg Ser Val Thr Arg Ala Ala Glu Lys 
            20                  25                  30          


Leu Phe Leu Gly Gln Pro Ala Ile Ser Ala Ala Leu Ser Arg Leu Arg 
        35                  40                  45              


Thr Leu Phe Asp Asp Pro Leu Phe Val Arg Thr Gly Arg Ser Met Glu 
    50                  55                  60                  


Pro Thr Ala Arg Ala Gln Glu Ile Phe Ala His Leu Ser Pro Ala Leu 
65                  70                  75                  80  


Asp Ser Ile Ser Thr Ala Met Ser Arg Ala Ser Glu Phe Asp Pro Ala 
                85                  90                  95      


Thr Ser Thr Ala Val Phe Arg Ile Gly Leu Ser Asp Asp Val Glu Phe 
            100                 105                 110         


Gly Leu Leu Pro Pro Leu Leu Arg Arg Leu Arg Ala Glu Ala Pro Gly 
        115                 120                 125             


Ile Val Leu Val Val Arg Arg Ala Asn Tyr Leu Leu Met Pro Asn Leu 
    130                 135                 140                 


Leu Ala Ser Gly Glu Ile Ser Val Gly Val Ser Tyr Thr Asp Glu Leu 
145                 150                 155                 160 


Pro Ala Asn Ala Lys Arg Lys Thr Val Arg Arg Ser Lys Pro Lys Ile 
                165                 170                 175     


Leu Arg Ala Asp Ser Ala Pro Gly Gln Leu Thr Leu Asp Asp Tyr Cys 
            180                 185                 190         


Ala Arg Pro His Ala Leu Val Ser Phe Ala Gly Asp Leu Ser Gly Phe 
        195                 200                 205             


Val Asp Glu Glu Leu Glu Lys Phe Gly Arg Lys Arg Lys Val Val Leu 
    210                 215                 220                 


Ala Val Pro Gln Phe Asn Gly Leu Gly Thr Leu Leu Ala Gly Thr Asp 
225                 230                 235                 240 


Ile Ile Ala Thr Val Pro Asp Tyr Ala Ala Gln Ala Leu Ile Ala Ala 
                245                 250                 255     


Gly Gly Leu Arg Ala Glu Asp Pro Pro Phe Glu Thr Arg Ala Phe Glu 
            260                 265                 270         


Leu Ser Met Ala Trp Arg Gly Ala Gln Asp Asn Asp Pro Ala Glu Arg 
        275                 280                 285             


Trp Leu Arg Ser Arg Ile Ser Met Phe Ile Gly Asp Pro Asp Ser Leu 
    290                 295                 300                 


<210>  3
<211>  916
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(916)
<223>  mexT P2

<400>  3
catgaaccga aacgacctgc gccgcgtcga tctgaacctg ctgatcgtgt tcgagaccct       60

gatgcacgaa cgcagcgtga cccgcgccgc agagaaactg ttcctcggcc agccggccat      120

cagcgccgcg ctgtagcgcc tgcgcacgct gttcgacgac ccgctgttcg tccgtaccgg      180

acgcagcatg gagcccaccg cgcgagccca ggaaatcttc gcccacctgt cgccggcgct      240

ggattccatc tccaccgcca tgagtcgcgc cagcgagttc gatccggcga ccagcaccgc      300

ggtgttccgc atcggccttt ccgacgacgt cgagttcggc ctgttgccgc ccctgctccg      360

ccgcctgcgc gcggaggcgc cggggatcgt cctcgtcgtg cgccgcgcca actatctatt      420

gatgccgaac ctgctggcct cgggggagat ctcggtgggc gtcagctaca ccgacgaact      480

gccggccaac gccaagcgca agaccgtgcg ccgcagcaag ccgaagatcc tccgcgccga      540

ctccgcgccc ggccagctga ccctcgacga ctattgcgcg cgaccgcacg cgctggtgtc      600

cttcgccggc gacctcagcg gcttcgtcga cgaggagctg gaaaaattcg gccgcaagcg      660

caaggtggtc ctggcggtgc cgcagttcaa cggcctcggc accctcctgg ccggcaccga      720

catcatcgcc accgtgcccg actacgccgc ccaggcgctg atcgccgccg gcggcctacg      780

cgccgaggac ccaccgttcg agacccgggc cttcgaactg tcgatggctt ggcgcggcgc      840

ccaggacaac gatccggccg aacgctggct gcgctcgcgg atcagcatgt tcatcggcga      900

tccggacagt ctctga                                                      916


<210>  4
<211>  44
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(44)
<223>  MexTP2 truncated

<400>  4

Met Asn Arg Asn Asp Leu Arg Arg Val Asp Leu Asn Leu Leu Ile Val 
1               5                   10                  15      


Phe Glu Thr Leu Met His Glu Arg Ser Val Thr Arg Ala Ala Glu Lys 
            20                  25                  30          


Leu Phe Leu Gly Gln Pro Ala Ile Ser Ala Ala Leu 
        35                  40                  


<210>  5
<211>  8
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  Sequence of eight base pair insertion in mexT of nfx-C strains of
       Pseudomonas aeruginosa.

<400>  5
cggccagc                                                                 8


<210>  6
<211>  10
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  6
acggccgacc                                                              10


<210>  7
<211>  10
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  7
agcgggccaa                                                              10


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide -- Forward primer for amplifying entire
       coding region of mexT from MPA01

<400>  8
cggataatga tcgggggtat                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide -- reverse primer for amplifying entire
       coding region of mexT from MPA01

<400>  9
ccgaattttt ccagctcctc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide -- Forward primer for verifying 
       transformants for correct PCR insertion

<400>  10
gcctgtcagt gatcctatgc                                                   20


<210>  11
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide -- Reverse primer for verifying 
       transformants for correct PCR insertion

<400>  11
gatcgccgat gaacatgc                                                     18


<210>  12
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide -- Forward primer for direct sequencing
       of amplified mexT in the P1 and P2 strains

<400>  12
gcctgtcagt gatcctatgc                                                   20


<210>  13
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide -- Reverse primer for direct sequencing
       of amplified mexT in the P1 and P2 strains

<400>  13
gatcgccgat gaacatgc                                                     18


