                         SEQUENCE LISTING

<110>  CEDARS-SINAI MEDICAL CENTER
       BHOWMICK, Neil A.
       CHOUDHURY, Diptiman
 
<120>  METHIONINE METABOLITES PREDICT AGGRESSIVE CANCER PROGRESSION

<130>  065472-000385WO00

<150>  61/682,155
<151>  2012-08-10

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  305
<212>  PRT
<213>  Helicobacter pylori 908

<400>  1

Met Ile Leu Thr Ala Met Gln Asp Ala Ile Gly Arg Thr Pro Ile Phe 
1               5                   10                  15      


Lys Phe Thr Arg Lys Asp Tyr Pro Ile Pro Leu Lys Ser Ala Ile Tyr 
            20                  25                  30          


Ala Lys Leu Glu His Leu Asn Pro Gly Gly Ser Val Lys Asp Arg Leu 
        35                  40                  45              


Gly Gln Tyr Leu Ile Lys Glu Ala Phe Arg Thr His Lys Ile Thr Ser 
    50                  55                  60                  


Thr Thr Thr Ile Ile Glu Pro Thr Ala Gly Asn Thr Gly Ile Ala Leu 
65                  70                  75                  80  


Ala Leu Val Ala Ile Lys His His Leu Lys Thr Ile Phe Val Val Pro 
                85                  90                  95      


Glu Lys Phe Ser Val Glu Lys Gln Gln Ile Met Arg Ala Leu Gly Ala 
            100                 105                 110         


Leu Val Ile Asn Thr Pro Thr Ser Glu Gly Ile Ser Gly Ala Ile Lys 
        115                 120                 125             


Lys Ser Lys Glu Leu Ala Glu Ser Ile Pro Asp Ser Tyr Leu Pro Leu 
    130                 135                 140                 


Gln Phe Glu Asn Pro Asp Asn Pro Ala Ala Tyr Tyr His Thr Leu Ala 
145                 150                 155                 160 


Pro Glu Ile Val Lys Glu Leu Gly Thr Asn Phe Thr Ser Phe Val Ala 
                165                 170                 175     


Gly Ile Gly Ser Gly Gly Thr Phe Ala Gly Thr Ala Lys Tyr Leu Lys 
            180                 185                 190         


Glu Arg Ile Pro Asn Ile Arg Leu Ile Gly Val Glu Pro Glu Gly Ser 
        195                 200                 205             


Ile Leu Asn Gly Gly Glu Pro Gly Pro His Glu Ile Glu Gly Ile Gly 
    210                 215                 220                 


Val Glu Phe Ile Pro Pro Phe Phe Ala Asn Leu Asp Ile Asp Gly Phe 
225                 230                 235                 240 


Glu Thr Ile Ser Asp Glu Glu Gly Phe Ser Tyr Thr Arg Lys Leu Ala 
                245                 250                 255     


Lys Lys Asn Gly Leu Leu Val Gly Ser Ser Ser Gly Ala Ala Phe Ala 
            260                 265                 270         


Ala Ala Leu Lys Glu Val Gln Arg Leu Pro Glu Gly Ser Gln Val Leu 
        275                 280                 285             


Thr Ile Phe Pro Asp Met Ala Asp Arg Tyr Leu Ser Lys Gly Ile Tyr 
    290                 295                 300                 


Ser 
305 


<210>  2
<211>  1215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  2
atgaccccgg tgtctggcaa cctgaaagtc gaattttaca actccaatcc gtctgatacc       60

acgaatagca ttaacccgca gttcaaagtt acgaacaccg gcagctctgc gattgatctg      120

tcaaaactga cgctgcgtta ttactatacc gtcgatggtc agaaagacca aaccttttgg      180

tgcgaccatg cggccattat cggtagtaac ggctcctaca atggcattac gtctaatgtc      240

aaaggcacct tcgtgaaaat gagttcctca acgaacaatg gcgccggtgc aggcgctatg      300

atcctgaccg cgatgcagga tgccatcggc cgtacgccga tttttaaatt cacccgcaaa      360

gactacccga tcccgctgaa aagtgcaatt tatgctaaac tggaacatct gaatccgggc      420

ggcagcgtga aagatcgtct gggtcaatat ctgattaaag aagcctttcg cacgcacaaa      480

atcaccagca ccacgaccat tatcgaaccg acggcgggta ataccggtat cgcactggcc      540

ctggttgcca ttaaacatca cctgaaaacc atctttgtgg ttccggaaaa attctcagtc      600

gaaaaacagc aaatcatgcg tgcgctgggc gccctggtga tcaacacgcc gacctcagaa      660

ggtatctcgg gcgcaattaa aaaatcgaaa gaactggctg aaagcattcc ggattcttac      720

ctgccgctgc aatttgaaaa cccggacaat ccggcagctt actatcatac cctggcaccg      780

gaaattgtga aagaactggg cacgaatttt accagcttcg ttgctggtat cggctctggc      840

ggtacgttcg caggcaccgc taaatatctg aaagaacgta ttccgaacat ccgcctgatt      900

ggcgtggaac cggaaggtag tattctgaat ggcggtgaac cgggtccgca cgaaatcgaa      960

ggtattggcg ttgaatttat cccgccgttt ttcgccaacc tggatattga cggctttgaa     1020

acgatttcag atgaagaagg tttctcgtat acccgcaaac tggcgaagaa aaacggtctg     1080

ctggttggca gcagcagcgg tgcagcattt gcagctgcgc tgaaagaagt tcagcgtctg     1140

ccggaaggca gccaagtcct gaccattttc ccggatatgg cggaccgcta cctgagtaaa     1200

ggtatctatt cctaa                                                      1215


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  3
ggcgccggtg caggcgct                                                     18


<210>  4
<211>  279
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  4
atgaccccgg tgtctggcaa cctgaaagtc gaattttaca actccaatcc gtctgatacc       60

acgaatagca ttaacccgca gttcaaagtt acgaacaccg gcagctctgc gattgatctg      120

tcaaaactga cgctgcgtta ttactatacc gtcgatggtc agaaagacca aaccttttgg      180

tgcgaccatg cggccattat cggtagtaac ggctcctaca atggcattac gtctaatgtc      240

aaaggcacct tcgtgaaaat gagttcctca acgaacaat                             279


<210>  5
<211>  404
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  5

Met Thr Pro Val Ser Gly Asn Leu Lys Val Glu Phe Tyr Asn Ser Asn 
1               5                   10                  15      


Pro Ser Asp Thr Thr Asn Ser Ile Asn Pro Gln Phe Lys Val Thr Asn 
            20                  25                  30          


Thr Gly Ser Ser Ala Ile Asp Leu Ser Lys Leu Thr Leu Arg Tyr Tyr 
        35                  40                  45              


Tyr Thr Val Asp Gly Gln Lys Asp Gln Thr Phe Trp Cys Asp His Ala 
    50                  55                  60                  


Ala Ile Ile Gly Ser Asn Gly Ser Tyr Asn Gly Ile Thr Ser Asn Val 
65                  70                  75                  80  


Lys Gly Thr Phe Val Lys Met Ser Ser Ser Thr Asn Asn Gly Ala Gly 
                85                  90                  95      


Ala Gly Ala Met Ile Leu Thr Ala Met Gln Asp Ala Ile Gly Arg Thr 
            100                 105                 110         


Pro Ile Phe Lys Phe Thr Arg Lys Asp Tyr Pro Ile Pro Leu Lys Ser 
        115                 120                 125             


Ala Ile Tyr Ala Lys Leu Glu His Leu Asn Pro Gly Gly Ser Val Lys 
    130                 135                 140                 


Asp Arg Leu Gly Gln Tyr Leu Ile Lys Glu Ala Phe Arg Thr His Lys 
145                 150                 155                 160 


Ile Thr Ser Thr Thr Thr Ile Ile Glu Pro Thr Ala Gly Asn Thr Gly 
                165                 170                 175     


Ile Ala Leu Ala Leu Val Ala Ile Lys His His Leu Lys Thr Ile Phe 
            180                 185                 190         


Val Val Pro Glu Lys Phe Ser Val Glu Lys Gln Gln Ile Met Arg Ala 
        195                 200                 205             


Leu Gly Ala Leu Val Ile Asn Thr Pro Thr Ser Glu Gly Ile Ser Gly 
    210                 215                 220                 


Ala Ile Lys Lys Ser Lys Glu Leu Ala Glu Ser Ile Pro Asp Ser Tyr 
225                 230                 235                 240 


Leu Pro Leu Gln Phe Glu Asn Pro Asp Asn Pro Ala Ala Tyr Tyr His 
                245                 250                 255     


Thr Leu Ala Pro Glu Ile Val Lys Glu Leu Gly Thr Asn Phe Thr Ser 
            260                 265                 270         


Phe Val Ala Gly Ile Gly Ser Gly Gly Thr Phe Ala Gly Thr Ala Lys 
        275                 280                 285             


Tyr Leu Lys Glu Arg Ile Pro Asn Ile Arg Leu Ile Gly Val Glu Pro 
    290                 295                 300                 


Glu Gly Ser Ile Leu Asn Gly Gly Glu Pro Gly Pro His Glu Ile Glu 
305                 310                 315                 320 


Gly Ile Gly Val Glu Phe Ile Pro Pro Phe Phe Ala Asn Leu Asp Ile 
                325                 330                 335     


Asp Gly Phe Glu Thr Ile Ser Asp Glu Glu Gly Phe Ser Tyr Thr Arg 
            340                 345                 350         


Lys Leu Ala Lys Lys Asn Gly Leu Leu Val Gly Ser Ser Ser Gly Ala 
        355                 360                 365             


Ala Phe Ala Ala Ala Leu Lys Glu Val Gln Arg Leu Pro Glu Gly Ser 
    370                 375                 380                 


Gln Val Leu Thr Ile Phe Pro Asp Met Ala Asp Arg Tyr Leu Ser Lys 
385                 390                 395                 400 


Gly Ile Tyr Ser 
                


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  6

Gly Ala Gly Ala Gly Ala 
1               5       


<210>  7
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  7

Met Thr Pro Val Ser Gly Asn Leu Lys Val Glu Phe Tyr Asn Ser Asn 
1               5                   10                  15      


Pro Ser Asp Thr Thr Asn Ser Ile Asn Pro Gln Phe Lys Val Thr Asn 
            20                  25                  30          


Thr Gly Ser Ser Ala Ile Asp Leu Ser Lys Leu Thr Leu Arg Tyr Tyr 
        35                  40                  45              


Tyr Thr Val Asp Gly Gln Lys Asp Gln Thr Phe Trp Cys Asp His Ala 
    50                  55                  60                  


Ala Ile Ile Gly Ser Asn Gly Ser Tyr Asn Gly Ile Thr Ser Asn Val 
65                  70                  75                  80  


Lys Gly Thr Phe Val Lys Met Ser Ser Ser Thr Asn Asn 
                85                  90              


<210>  8
<211>  378
<212>  PRT
<213>  Helicobacter pylori 908

<400>  8

Met Gln Thr Lys Leu Ile His Gly Gly Ile Ser Glu Asp Ala Thr Thr 
1               5                   10                  15      


Gly Ala Val Ser Val Pro Ile Tyr Gln Ala Ser Thr Tyr Arg Gln Asp 
            20                  25                  30          


Ala Ile Gly Arg His Lys Gly Tyr Glu Tyr Ser Arg Ser Gly Asn Pro 
        35                  40                  45              


Thr Arg Phe Ala Leu Glu Glu Leu Ile Ala Asp Leu Glu Gly Gly Val 
    50                  55                  60                  


Lys Gly Phe Ala Phe Ala Ser Gly Leu Ala Gly Ile His Ala Val Phe 
65                  70                  75                  80  


Ser Leu Leu Gln Ser Gly Asp His Val Leu Leu Gly Asp Asp Val Tyr 
                85                  90                  95      


Gly Gly Thr Phe Arg Leu Phe Asn Lys Val Leu Val Lys Asn Gly Leu 
            100                 105                 110         


Ser Cys Thr Ile Ile Asp Thr Ser Asp Ile Ser Gln Ile Lys Lys Ala 
        115                 120                 125             


Ile Lys Pro Asn Thr Lys Ala Leu Tyr Leu Glu Thr Pro Ser Asn Pro 
    130                 135                 140                 


Leu Leu Lys Ile Thr Asp Leu Ala Gln Cys Ala Ser Val Ala Lys Asp 
145                 150                 155                 160 


His Gly Leu Leu Thr Ile Val Asp Asn Thr Phe Ala Thr Pro Tyr Cys 
                165                 170                 175     


Gln Asn Pro Leu Leu Leu Gly Ala Asp Ile Val Ala His Ser Gly Thr 
            180                 185                 190         


Lys Tyr Leu Gly Gly His Ser Asp Val Val Ala Gly Leu Val Thr Thr 
        195                 200                 205             


Asn Asn Glu Ala Leu Ala Gln Glu Ile Ala Phe Phe Gln Asn Ala Ile 
    210                 215                 220                 


Gly Gly Val Leu Gly Pro Gln Asp Ser Trp Leu Leu Gln Arg Gly Ile 
225                 230                 235                 240 


Lys Thr Leu Gly Leu Arg Met Glu Ala His Gln Lys Asn Ala Leu Cys 
                245                 250                 255     


Val Ala Glu Phe Leu Glu Lys His Pro Lys Val Glu Arg Val Tyr Tyr 
            260                 265                 270         


Pro Gly Leu Pro Thr His Pro Asn His Glu Leu Ala Lys Ala Gln Met 
        275                 280                 285             


Arg Gly Phe Ser Gly Met Leu Ser Phe Thr Leu Lys Asn Asp Ser Glu 
    290                 295                 300                 


Ala Ala Leu Phe Val Glu Ser Leu Lys Leu Phe Ile Leu Gly Glu Ser 
305                 310                 315                 320 


Leu Gly Gly Val Glu Ser Leu Val Gly Ile Pro Ala Leu Met Thr His 
                325                 330                 335     


Ala Cys Ile Pro Lys Glu Gln Arg Glu Ala Ala Gly Ile Arg Asp Gly 
            340                 345                 350         


Leu Val Arg Leu Ser Val Gly Ile Glu His Glu Gln Asp Leu Leu Glu 
        355                 360                 365             


Asp Leu Glu Gln Ala Phe Ala Lys Ile Ser 
    370                 375             


<210>  9
<211>  1434
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  9
atgacgccgg tgtctggcaa tctgaaagtg gaattttaca acagcaaccc gagcgatacg       60

acgaatagca tcaacccgca gttcaaagtg accaacacgg gtagctctgc gattgatctg      120

tctaaactga ccctgcgtta ttactatacg gttgatggcc agaaagacca aaccttttgg      180

tgcgaccatg cggccattat cggttctaac ggcagttata atggtatcac cagcaatgtg      240

aaaggcacgt tcgttaaaat gagttcctca accaacaatg gcgcaggtgc tggcgcgatg      300

cagacgaaac tgattcatgg cggtatcagc gaagatgcaa ccacgggtgc agtctcggtg      360

ccgatttacc aggccagcac ctatcgtcaa gacgcaatcg gtcgccacaa aggctacgaa      420

tattcgcgta gcggtaaccc gacgcgcttt gcactggaag aactgattgc ggatctggaa      480

ggcggtgtga aaggctttgc cttcgcatca ggtctggcag gcatccatgc tgttttctcg      540

ctgctgcaaa gcggtgacca cgtcctgctg ggcgatgacg tgtacggcgg cacctttcgc      600

ctgttcaaca aagttctggt caaaaatggt ctgagttgta ccattatcga tacgtccgac      660

atttcacaga tcaaaaaagc gattaaaccg aacaccaaag ccctgtatct ggaaacgccg      720

tcgaatccgc tgctgaaaat taccgatctg gcccagtgcg caagcgttgc taaagatcat      780

ggcctgctga cgatcgtgga taacaccttt gcgacgccgt actgtcaaaa tccgctgctg      840

ctgggtgcgg atattgtcgc ccattccggc accaaatatc tgggcggtca ctcagacgtg      900

gttgccggtc tggttaccac gaacaatgaa gctctggcgc aggaaattgc gtttttccaa      960

aacgcaatcg gcggtgtgct gggtccgcag gatagctggc tgctgcaacg tggtatcaaa     1020

accctgggcc tgcgcatgga agcgcatcag aaaaatgcac tgtgcgttgc tgaatttctg     1080

gaaaaacacc cgaaagtgga acgtgtttac tatccgggtc tgccgaccca tccgaaccac     1140

gaactggcca aagcacaaat gcgcggtttt tctggcatgc tgagtttcac gctgaaaaat     1200

gattctgaag cagctctgtt tgtggaaagt ctgaaactgt tcattctggg tgaatccctg     1260

ggcggtgtcg aatcactggt gggcattccg gcactgatga cccatgcttg tatcccgaaa     1320

gaacagcgtg aagcggccgg tattcgtgat ggcctggttc gcctgtctgt cggcatcgaa     1380

cacgaacagg atctgctgga agacctggaa caggcgtttg cgaaaattag ttaa           1434


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  10
ggcgcaggtg ctggcgcg                                                     18


<210>  11
<211>  279
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  11
atgacgccgg tgtctggcaa tctgaaagtg gaattttaca acagcaaccc gagcgatacg       60

acgaatagca tcaacccgca gttcaaagtg accaacacgg gtagctctgc gattgatctg      120

tctaaactga ccctgcgtta ttactatacg gttgatggcc agaaagacca aaccttttgg      180

tgcgaccatg cggccattat cggttctaac ggcagttata atggtatcac cagcaatgtg      240

aaaggcacgt tcgttaaaat gagttcctca accaacaat                             279


<210>  12
<211>  477
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic contruct

<400>  12

Met Val Ser Tyr Lys Cys Gly Val Lys Asp Gly Thr Lys Asn Thr Ile 
1               5                   10                  15      


Arg Ala Thr Ile Asn Ile Lys Asn Thr Gly Thr Thr Pro Val Asn Leu 
            20                  25                  30          


Ser Asp Ile Lys Val Arg Tyr Trp Phe Thr Ser Asp Gly Glu Asn Asn 
        35                  40                  45              


Phe Val Cys Asp Tyr Ala Ala Phe Gly Thr Asp Lys Val Lys Lys Lys 
    50                  55                  60                  


Ile Glu Asn Ser Val Pro Gly Ala Asp Thr Tyr Cys Glu Ile Ser Val 
65                  70                  75                  80  


Lys Gly Thr Phe Val Lys Met Ser Ser Ser Thr Asn Asn Gly Ala Gly 
                85                  90                  95      


Ala Gly Ala Met Gln Thr Lys Leu Ile His Gly Gly Ile Ser Glu Asp 
            100                 105                 110         


Ala Thr Thr Gly Ala Val Ser Val Pro Ile Tyr Gln Ala Ser Thr Tyr 
        115                 120                 125             


Arg Gln Asp Ala Ile Gly Arg His Lys Gly Tyr Glu Tyr Ser Arg Ser 
    130                 135                 140                 


Gly Asn Pro Thr Arg Phe Ala Leu Glu Glu Leu Ile Ala Asp Leu Glu 
145                 150                 155                 160 


Gly Gly Val Lys Gly Phe Ala Phe Ala Ser Gly Leu Ala Gly Ile His 
                165                 170                 175     


Ala Val Phe Ser Leu Leu Gln Ser Gly Asp His Val Leu Leu Gly Asp 
            180                 185                 190         


Asp Val Tyr Gly Gly Thr Phe Arg Leu Phe Asn Lys Val Leu Val Lys 
        195                 200                 205             


Asn Gly Leu Ser Cys Thr Ile Ile Asp Thr Ser Asp Ile Ser Gln Ile 
    210                 215                 220                 


Lys Lys Ala Ile Lys Pro Asn Thr Lys Ala Leu Tyr Leu Glu Thr Pro 
225                 230                 235                 240 


Ser Asn Pro Leu Leu Lys Ile Thr Asp Leu Ala Gln Cys Ala Ser Val 
                245                 250                 255     


Ala Lys Asp His Gly Leu Leu Thr Ile Val Asp Asn Thr Phe Ala Thr 
            260                 265                 270         


Pro Tyr Cys Gln Asn Pro Leu Leu Leu Gly Ala Asp Ile Val Ala His 
        275                 280                 285             


Ser Gly Thr Lys Tyr Leu Gly Gly His Ser Asp Val Val Ala Gly Leu 
    290                 295                 300                 


Val Thr Thr Asn Asn Glu Ala Leu Ala Gln Glu Ile Ala Phe Phe Gln 
305                 310                 315                 320 


Asn Ala Ile Gly Gly Val Leu Gly Pro Gln Asp Ser Trp Leu Leu Gln 
                325                 330                 335     


Arg Gly Ile Lys Thr Leu Gly Leu Arg Met Glu Ala His Gln Lys Asn 
            340                 345                 350         


Ala Leu Cys Val Ala Glu Phe Leu Glu Lys His Pro Lys Val Glu Arg 
        355                 360                 365             


Val Tyr Tyr Pro Gly Leu Pro Thr His Pro Asn His Glu Leu Ala Lys 
    370                 375                 380                 


Ala Gln Met Arg Gly Phe Ser Gly Met Leu Ser Phe Thr Leu Lys Asn 
385                 390                 395                 400 


Asp Ser Glu Ala Ala Leu Phe Val Glu Ser Leu Lys Leu Phe Ile Leu 
                405                 410                 415     


Gly Glu Ser Leu Gly Gly Val Glu Ser Leu Val Gly Ile Pro Ala Leu 
            420                 425                 430         


Met Thr His Ala Cys Ile Pro Lys Glu Gln Arg Glu Ala Ala Gly Ile 
        435                 440                 445             


Arg Asp Gly Leu Val Arg Leu Ser Val Gly Ile Glu His Glu Gln Asp 
    450                 455                 460                 


Leu Leu Glu Asp Leu Glu Gln Ala Phe Ala Lys Ile Ser 
465                 470                 475         


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  13

Gly Ala Gly Ala Gly Ala 
1               5       


<210>  14
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic contruct

<400>  14

Met Val Ser Tyr Lys Cys Gly Val Lys Asp Gly Thr Lys Asn Thr Ile 
1               5                   10                  15      


Arg Ala Thr Ile Asn Ile Lys Asn Thr Gly Thr Thr Pro Val Asn Leu 
            20                  25                  30          


Ser Asp Ile Lys Val Arg Tyr Trp Phe Thr Ser Asp Gly Glu Asn Asn 
        35                  40                  45              


Phe Val Cys Asp Tyr Ala Ala Phe Gly Thr Asp Lys Val Lys Lys Lys 
    50                  55                  60                  


Ile Glu Asn Ser Val Pro Gly Ala Asp Thr Tyr Cys Glu Ile Ser Val 
65                  70                  75                  80  


Lys Gly Thr Phe Val Lys Met Ser Ser Ser Thr Asn Asn 
                85                  90              


