                         SEQUENCE LISTING

<110>  Helmholtz Zentrum Mnchen
 
<120>  Novel Far Red Fluorescent Protein

<130>  T2100 PCT

<150>  EP 12 17 9959.7
<151>  2012-08-09

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amrose v1

<400>  1

Met Ser Glu Leu Ile Lys Glu Asn Met His Met Lys Leu Tyr Met Glu 
1               5                   10                  15      


Gly Thr Val Asn Asn His His Phe Lys Cys Thr Ser Glu Gly Glu Gly 
            20                  25                  30          


Lys Pro Tyr Glu Gly Thr Gln Thr Met Lys Ile Lys Val Val Glu Gly 
        35                  40                  45              


Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr 
    50                  55                  60                  


Gly Ser Lys Ala Phe Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe 
65                  70                  75                  80  


Lys Glu Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg Ile Thr Thr Tyr 
                85                  90                  95      


Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asn 
            100                 105                 110         


Gly Cys Leu Ile Tyr Asn Val Lys Ile Asn Gly Val Asn Phe Pro Ser 
        115                 120                 125             


Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr 
    130                 135                 140                 


Glu Met Met Tyr Pro Ala Asp Ser Gly Leu Arg Gly His Asn Gln Met 
145                 150                 155                 160 


Ala Leu Lys Leu Val Gly Gly Gly Tyr Leu His Cys Ser Leu Lys Thr 
                165                 170                 175     


Thr Tyr Arg Ser Lys Lys Pro Ala Lys Asn Leu Lys Met Pro Gly Phe 
            180                 185                 190         


His Phe Val Asp Tyr Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu 
        195                 200                 205             


Thr Tyr Val Glu Gln His Glu Met Ala Val Ala Lys Tyr Cys Asp Leu 
    210                 215                 220                 


Pro Ser Lys Leu Gly His Arg 
225                 230     


<210>  2
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amrose v1

<400>  2
atgagcgagc tgatcaagga gaacatgcac atgaaactgt acatggaggg caccgtgaac       60

aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc      120

atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc      180

agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt      240

aaggagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc      300

gtgctgaccg ctacccagga caccagcctc cagaacggct gcctcatcta caacgtcaag      360

atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg      420

gaggccagca ccgagatgat gtaccccgct gacagcggcc tgagaggcca taaccagatg      480

gccctgaagc tcgtgggcgg gggctacctg cactgctccc tcaagaccac atacagatcc      540

aagaaacccg ctaagaacct caagatgccc ggcttccact tcgtggacta cagactggaa      600

agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag      660

tactgcgacc tccctagcaa actggggcac agatga                                696


<210>  3
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amrose v2

<400>  3

Met Ser Glu Leu Ile Lys Glu Asn Met His Met Lys Leu Tyr Met Glu 
1               5                   10                  15      


Gly Thr Val Asn Asn His His Phe Lys Cys Thr Ser Glu Gly Glu Gly 
            20                  25                  30          


Lys Pro Tyr Glu Gly Thr Gln Thr Met Lys Ile Lys Val Val Glu Gly 
        35                  40                  45              


Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr 
    50                  55                  60                  


Gly Ser Lys Ala Phe Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe 
65                  70                  75                  80  


Lys Glu Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg Ile Thr Thr Tyr 
                85                  90                  95      


Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asn 
            100                 105                 110         


Gly Cys Leu Ile Tyr Asn Val Lys Ile Asn Gly Val Asp Phe Pro Ser 
        115                 120                 125             


Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr 
    130                 135                 140                 


Glu Met Met Tyr Pro Ala Asp Ser Gly Leu Arg Gly His Asn Gln Met 
145                 150                 155                 160 


Ala Leu Lys Leu Val Gly Gly Gly Tyr Leu His Cys Ser Leu Lys Thr 
                165                 170                 175     


Thr Tyr Arg Ser Lys Lys Pro Ala Asn Asn Leu Lys Met Pro Gly Phe 
            180                 185                 190         


His Phe Val Asp Tyr Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu 
        195                 200                 205             


Thr Tyr Val Glu Gln His Glu Met Ala Val Ala Lys Tyr Cys Asp Leu 
    210                 215                 220                 


Pro Ser Lys Leu Gly His Arg 
225                 230     


<210>  4
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amrose v2

<400>  4
atgagcgagc tgatcaagga gaacatgcac atgaaactgt acatggaggg caccgtgaac       60

aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc      120

atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc      180

agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt      240

aaggagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc      300

gtgctgaccg ctacccagga caccagcctc cagaacggct gcctcatcta caacgtcaag      360

atcaacgggg tggacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg      420

gaggccagca ccgagatgat gtaccccgct gacagcggcc tgagaggcca taaccagatg      480

gccctgaagc tcgtgggcgg gggctacctg cactgctccc tcaagaccac atacagatcc      540

aagaaacccg ctaacaacct caagatgccc ggcttccact tcgtggacta cagactggaa      600

agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag      660

tactgcgacc tccctagcaa actggggcac agatga                                696


<210>  5
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amrose v3

<400>  5

Met Ser Glu Leu Ile Lys Glu Asn Met His Met Lys Leu Tyr Met Gly 
1               5                   10                  15      


Gly Thr Val Asn Asn His His Phe Lys Cys Thr Ser Glu Gly Glu Gly 
            20                  25                  30          


Lys Pro Tyr Glu Gly Thr Gln Thr Met Lys Ile Lys Val Val Glu Gly 
        35                  40                  45              


Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr 
    50                  55                  60                  


Gly Ser Lys Ala Phe Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe 
65                  70                  75                  80  


Lys Glu Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg Ile Thr Thr Tyr 
                85                  90                  95      


Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asn 
            100                 105                 110         


Gly Cys Leu Ile Tyr Asn Val Lys Ile Asn Gly Val Asn Phe Pro Ser 
        115                 120                 125             


Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr 
    130                 135                 140                 


Glu Met Met Tyr Pro Ala Asp Ser Gly Leu Arg Gly His Asn Gln Met 
145                 150                 155                 160 


Ala Leu Lys Leu Val Gly Gly Gly Tyr Leu His Cys Ser Leu Lys Thr 
                165                 170                 175     


Thr Tyr Arg Ser Lys Lys Pro Ala Lys Asn Leu Lys Met Pro Gly Phe 
            180                 185                 190         


His Phe Val Asp Tyr Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu 
        195                 200                 205             


Thr Tyr Val Glu Gln His Glu Met Ala Val Ala Lys Tyr Cys Asp Leu 
    210                 215                 220                 


Pro Ser Lys Leu Gly His Arg 
225                 230     


<210>  6
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amrose v3

<400>  6
atgagcgagc tgatcaagga gaacatgcac atgaaactgt acatgggggg caccgtgaac       60

aaccaccact tcaagtgtac atccgagggc gaaggcaagc cctacgaggg cacccagacc      120

atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc      180

agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt      240

aaggagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc      300

gtgctgaccg ctacccagga caccagcctc cagaacggct gcctcatcta caacgtcaag      360

atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg      420

gaggccagca ccgagatgat gtaccccgct gacagcggcc tgagaggcca taaccagatg      480

gccctgaagc tcgtgggcgg gggctacctg cactgctccc tcaagaccac atacagatcc      540

aagaaacccg ctaagaacct caagatgccc ggcttccact tcgtggacta cagactggaa      600

agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag      660

tactgcgacc tccctagcaa actggggcac agatga                                696


<210>  7
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amrose v4

<400>  7

Met Ser Glu Leu Ile Lys Glu Asn Met His Met Lys Leu Tyr Met Gly 
1               5                   10                  15      


Gly Thr Val Asn Asn His His Phe Lys Cys Thr Ser Glu Gly Glu Gly 
            20                  25                  30          


Lys Pro Tyr Glu Gly Thr Gln Thr Met Lys Ile Lys Val Val Glu Gly 
        35                  40                  45              


Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr 
    50                  55                  60                  


Gly Ser Lys Ala Phe Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe 
65                  70                  75                  80  


Lys Glu Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg Ile Thr Thr Tyr 
                85                  90                  95      


Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asn 
            100                 105                 110         


Gly Cys Leu Ile Tyr Asn Val Lys Ile Asn Gly Val Asp Phe Pro Ser 
        115                 120                 125             


Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr 
    130                 135                 140                 


Glu Met Met Tyr Pro Ala Asp Ser Gly Leu Arg Gly His Asn Gln Met 
145                 150                 155                 160 


Ala Leu Lys Leu Val Gly Gly Gly Tyr Leu His Cys Ser Leu Lys Thr 
                165                 170                 175     


Thr Tyr Arg Ser Lys Lys Pro Ala Asn Asn Leu Lys Met Pro Gly Phe 
            180                 185                 190         


His Phe Val Asp Tyr Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu 
        195                 200                 205             


Thr Tyr Val Glu Gln His Glu Met Ala Val Ala Lys Tyr Cys Asp Leu 
    210                 215                 220                 


Pro Ser Lys Leu Gly His Arg 
225                 230     


<210>  8
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amrose v4

<400>  8
atgagcgagc tgatcaagga gaacatgcac atgaaactgt acatgggggg caccgtgaac       60

aaccaccact tcaagtgtac atccgagggc gaaggcaagc cctacgaggg cacccagacc      120

atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc      180

agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt      240

aaggagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc      300

gtgctgaccg ctacccagga caccagcctc cagaacggct gcctcatcta caacgtcaag      360

atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg      420

gaggccagca ccgagatgat gtaccccgct gacagcggcc tgagaggcca taaccagatg      480

gccctgaagc tcgtgggcgg gggctacctg cactgctccc tcaagaccac atacagatcc      540

aagaaacccg ctaagaacct caagatgccc ggcttccact tcgtggacta cagactggaa      600

agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag      660

tactgcgacc tccctagcaa actggggcac agatga                                696


<210>  9
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer AI24

<400>  9
cccagatctt gcttgtgaag tcttcttatt gctg                                   34


<210>  10
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer AI25

<400>  10
cccgctagcg ccaccatgga cagcctcttg atgaagagga                             40


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer rbc580

<400>  11
gtgtgttgga ggtcgctgag tagtgcgcga gc                                     32


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer rbc271

<400>  12
ggctgattat gatcctctag agtcg                                             25


<210>  13
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer rbc584

<400>  13
agacgggtct gacatggatt ggacgaa                                           27


<210>  14
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer rbc276

<400>  14
ccgcccgggt cggcttcggt cggagccatg gagatc                                 36


