                         SEQUENCE LISTING

<110>  INSERM
       Payrastre, Bernard
 
<120>  Method and Reagents for the Quantification of the Lipid 
       Phosphatidylinositol 3 Monophosphate

<130>  BCT120297 QT

<150>  EP12305994.1
<151>  2012-08-09

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sense primer

<400>  1
atggccacag atgacaagag ttcc                                              24


<210>  2
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Antisence primer

<400>  2
tcagcaattc agatccaacc ctgccc                                            26


<210>  3
<211>  11615
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector for the production of recombinant GST-PYKfyve

<400>  3
tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt       60

ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca      120

ccatcgggcg cggatctcgg tccgaaggag gcagatcatg tcccctatac taggttattg      180

gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata      240

tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt      300

gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc      360

tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga      420

gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag      480

aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga      540

aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt      600

aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat      660

gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca      720

aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc      780

cacgtttggt ggtggcgacc atcctccaaa atcggatctg gttccgcgtg aaaaccttta      840

cttccagggc cacggatcca tgggacatat gctcgagcta gcaggcctgc agtcgactat      900

ggccacagat gacaagagtt ccccgacact ggactctgct aatgatttgc ctcgctctcc      960

tgccagtcct tctcacctca ctcactttaa acccttgact cctgaccagg atgagccccc     1020

cttcaagtca gcatatagtt cttttgtaaa cctttttcgt tttaacaaag agcgaggaga     1080

agggggccaa ggagagcagc agtctccgag ttcaagttgg gccagccctc agatcccttc     1140

aagaacacag tctgtgaggt cgcctgtacc ttataaaaaa cagcttaatg aggagctcca     1200

ccggcgctct tcagtgttag actccagaag gaaagcagaa ccagcctgtg gaggtcatga     1260

cccacgtaca gctgttcagc ttcgaagcct cagcacagta ttgaaacgcc tcaaagaaat     1320

catggaagga aaaagccagg acagtgacct gaagcaatat tggatgccag atagccagtg     1380

taaagagtgc tatgactgca gtgaaaagtt tacaacattt aggcgcagac accattgcag     1440

actgtgtggg cagattttct gcagtcgttg ttgtaatcaa gaaatccctg gaaaatttat     1500

gggctataca ggagacctcc gagcatgcac ctactgtaga aaaatagcct taagttatgc     1560

tcattctaca gacagtaatt ccattgggga agacttgaat gctctttcag attcaacttg     1620

ctctgtgtct atacttgatc caagcgaacc tcggacacca gttgggagta gaaaagccag     1680

tcgtaacata ttcttagagg atgatttagc ctggcaaagc ttgattcatc cggattcctc     1740

aaatagtgct ctttcaacaa gactcgtatc tgttcaagag gatgctggga agtctcctgc     1800

tcgaaacaga tcagccagca ttactaatct gtcactggat cggtctggtt ctcctatggt     1860

tccttcatat gagacatctg tcagtcccca ggctaaccga aactacatta ggacagagac     1920

gactgaggat gaacgcaaaa ttcttctgga cagtgctcag ttaaaggatc tgtggaagaa     1980

aatctgccat cacaccagtg ggatggaatt tcaagatcac cgttactggt tgagaacaca     2040

tcccaactgc attgtaggga aggaattagt caactggcta atcagaaatg gacacatcgc     2100

tacaagggca caagctatag caattggaca agcaatggtt gatggacgtt ggttggattg     2160

tgttagtcat catgatcagc ttttcaggga cgaatatgcg ttgtatagac cacttcagag     2220

tacagaattt tctgagacac cttctccaga cagtgactct gtgaactctg tggaaggaca     2280

ctccgagcca tcctggttta aagacataaa atttgatgac agtgacacag aacagattgc     2340

tgaagaaggt gacgataatt tggctaagta tttggtttct gacactggag gacagcagct     2400

ctcaataagt gatgccttca tcaaagagtc cttatttaat cgacgagtag aggaaaaatc     2460

caaagagctg ccttttaccc ctttgggctg gcatcataac aacctggaac tcttgcgaga     2520

ggagaatgag gagaagcaag ccatggaaag gctgctttca gctaatcata accacatgat     2580

ggccctactc cagcagttgc ttcaaaacga gtcattgtca tcgtcttgga gggacatcat     2640

tgtgtcacta gtctgccagg ttgttcagac agtccgacct gatgtcaagc accaggatga     2700

tgacatggat atccgtcagt ttgtccatat caagaagatc ccaggtggaa agaaatttga     2760

ctctgtggtt gtcaatggct ttgtttgtac caagaacatt gcacataaaa agatgaattc     2820

ctgtattaaa aaccccaaaa tccttctgtt gaagtgttct attgagtatc tctatagaga     2880

agaaactaag tttacctgca ttgatcctat tgtgcttcag gaaagggaat tcttgaagaa     2940

ttatgttcaa cgaatagttg atgttcgacc cacattggtt cttgttgaga aaacagtgtc     3000

tcggattgct caggacatgt tactggaaca tggcattact ctggtcatta atgtaaagtc     3060

acaagtttta gaaagaatca gtcgaatgac ccaaggtgat ttagtggtgt ccatggacca     3120

gctgctcacc aaaccccact tgggcacttg ccacaaattt tatatgcaga tatttcagct     3180

gcctaatgaa caaaccaaaa cactgatgtt ttttgaaggc tgtccacagc atctaggctg     3240

cacaatcaag ctcagaggag gctctgatta tgagctggct cgagttaagg agatcctaat     3300

atttatgatc tgtgtagctt atcattctca gctagaaatc tcttttctca tggatgagtt     3360

cgctatgcct ccaacattaa tgcaaagccc ttcattccat cttctgacgg agggacgagg     3420

tgaagaggga gcctctcagg agcaggtcag tggcagctcc cttcctcagg atcctgagtg     3480

ccctcgtgag gccctgtctt ctgaggatag cactttgttg gaatcaagga ctgtgctaga     3540

gaagggtgaa ctagacaata aaagtattcc acaagctgtt gcctctttga agcatcaaga     3600

ttataccacc cccacttgcc cagcaggtat tccctgtgct ctttttgcat tggtaccaga     3660

gtcattgttg cctctccata tggatcaaca ggatgccgta ggaaatgaac agccagagac     3720

ttcacagcaa acggatgagc aacaggatcc caaaggccag atgaaagctt ttagcgaccc     3780

tttacaggat gacactggaa tgtacgttac tgaggaagtc acctcctctg aagatcaacg     3840

aaagacttat gccttgacat ttaaacagga gttaaaagat gtaatcctct gtatctctcc     3900

agttattaca ttccgtgaac ctttcctttt aactgaaaag gggatgagat gctcaactcg     3960

agattatttt ccagagcaga tttactggtc tcctcttctc aacaaagagg tgaaggaaat     4020

ggagagcagg aggaagaaac agctgctcag ggatctctct ggacttcagg gcatgaatgg     4080

cagtgttcag gccaagtcta ttcaagtctt accctcacat gagctagtga gcaccaggat     4140

tgctgaacat ctgggtgaca gccagacctt gggtagaatg ctagctgatt atcgagctag     4200

aggaggaaga attcagtcaa aacatttgga cccctttgtc cattcaaaag atgcatcatg     4260

tacttcaggt ggcaaatcag gaaacaaaac tgagagtgat gaagagaggg gattgattcc     4320

aagtgatgta atatggccaa caaaggtgga ctgtctgaac cctgctaacc accagaggct     4380

ctgtgtgctc ttcagcggct cttctgccca gtccagcaat gctcccagtg cttgtgtcag     4440

tccttggatt gtaacaatgg agttttatgg aaagaatgac cttacactgg ggatattttt     4500

agaaagatac tgtttcaggt cttcttacca gtgtccgagc atgttctgtg acacccccat     4560

ggttcatcac attcgacgct ttgttcatgg ccaaggctgt gtacagataa ttctgaagga     4620

gttggattct ccagtgcctg gatatcaaca tacaattctc acatattcct ggtgcagaat     4680

ctgcaaacaa gtaacaccag ttgttgctct ttcaaatgaa tcctggtcta tgtcatttgc     4740

aaagtacctt gaacttcgat tttatggcca ccagtacaca cgcagagcca acgctgagcc     4800

ctgcggtcac tctatccacc atgattatca tcagtatttc tcttataacc agatggtggc     4860

atctttcagt tactctccta ttcggcttct tgaagtatgt gttccactac caaaaatatt     4920

cattaagcgt caagccccac tgaaggtatc tcttcttcag gacctcaaag acttttttca     4980

gaaggtttca caggtgtacc tagctgttga tgagagactt gcatccttga aaacggatac     5040

atttagcaaa actagagagg aaaagatgga agatatcttt gcacaaaagg agatggaaga     5100

gggtgagttt aagaactgga cagagaagat gcaagcaagg ctcatgtctt cctctgtgga     5160

tacccctcag caactgcagt ccatttttga gtcactgatt gccaagaagc aaagcctctg     5220

tgaggtgctc caggcgtgga acagcaggtt gcaggacctc ttccagcagg aaaaaggtag     5280

aaagaggcct tcagttcctc ccagtcctgg gagactgaga caaggtgaag aaagcaagat     5340

aaatgcaatg gacacatctc caaggaatat ttctccagga cttcacaatg gagaaaaaga     5400

ggatcgcttc ttgacaaccc tgtccagcca gagctctacg agctccaccc acctccagct     5460

gcccactcct cccgaggccc tggccgagca ggtagtggga gggccgactg atctggattc     5520

agccagtggc tctgaagatg tatttgatgg tcatttgctg ggatccacag acagccaggt     5580

gaaggaaaag tcaaccatga aagccatctt tgctaatttg cttccaggaa acagctacaa     5640

tcccattcca tttccttttg atccagataa acactactta atgtatgaac atgaacgggt     5700

gcccattgct gtctgtgaga aagagcccag ctccatcatt gcttttgcac tcagttgtaa     5760

agaataccgc aatgccttag aggaattgtc caaagcaact ctgcggaaca gtgctgaaga     5820

agggctccca gccaatagtg ctttagataa cagacctaag agtagcagcc ctattagact     5880

acctgaaatc agtggaggac agacaaaccg cacagtagaa gcagaacctc agccaaccaa     5940

aaaagcttca ggaatgttgt ccttcttcag aggaacagca gggaagagcc ctgatctgtc     6000

ttcccagaag agggagacct tgcgaggggc agacagtgct tactaccagg ttgggcaggc     6060

cggcaaggag gggttggaga gtcaaggcct ggagcctcaa gatgaagtag atggaggaga     6120

tacacagaag aaacaactca caaatcctca tgtggaactt caattttctg atgctaatgc     6180

caagttttac tgtcggctgt actacgcggg agagttccac aagatgcgtg aagtgattct     6240

gggcagcagt gaggaggaat tcatccgttc cctttctcac tcatctccct ggcaggcccg     6300

gggaggcaag tcaggagctg ctttctatgc caccgaagat gatagattca ttctgaagca     6360

aatgcctcgt ttggaagtcc agtctttcct tgactttgca ccacactact tcaattatat     6420

cacaaatgct gttcaacaaa agaggcccac cgccttggct aaaattcttg gagtttacag     6480

aattggttat aagaactctc agaacaacac tgagaagaag ttagatctcc ttgtcatgga     6540

aaatcttttc tatgggagaa agatggcaca ggtttttgat ttgaagggtt cacttaggaa     6600

tcgaaatgta aaaactgaca ctgggaaaga gagctgtgat gtggttctgt tggatgaaaa     6660

cctcctaaag atggttcgag acaaccctct ctatattcgt tcccattcca aatctgtgct     6720

gagaacctcc atccacagcg acgcccattt cctttccagc cacctcatta tagactattc     6780

tctgctggtt gggcgagatg acactagcaa tgagcttgtg gttggcatca tagattacat     6840

tcgaacattt acatgggaca aaaaacttga gatggttgtg aagtcaacag gaattttagg     6900

aggacaaggt aaaatgccaa ctgtggtctc tccagagttg tataggacta gattttgtga     6960

agcaatggac aagtatttct tgatggtgcc agaccactgg acagggttgg atctgaattg     7020

ctgaaagggc aattctgcag atatccagca cagtggcggc cgctcgagtc tagaattctt     7080

taattaaaag tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc     7140

tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt     7200

tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt     7260

cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt     7320

atcttatcat gtctggatct gatcactgct tgagcctagg agatccgaac cagataagtg     7380

aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac     7440

ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc     7500

cctcccagtt cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt     7560

tttaatcaaa catcctgcca actccatgtg acaaaccgtc atcttcggct actttttctc     7620

tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt aatgtttgta attgactgaa     7680

tatcaacgct tatttgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa     7740

gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc     7800

ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag     7860

ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca     7920

aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc     7980

gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa     8040

cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct     8100

attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa     8160

cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat     8220

ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc     8280

aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct     8340

tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag     8400

atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta     8460

agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc     8520

tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca     8580

tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg     8640

atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg     8700

ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca     8760

tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa     8820

acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa     8880

ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata     8940

aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat     9000

ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc     9060

cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata     9120

gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt     9180

actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga     9240

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag     9300

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa     9360

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag     9420

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg     9480

tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat     9540

acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta     9600

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg     9660

gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc     9720

gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa     9780

gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc     9840

tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt     9900

caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct     9960

tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc    10020

gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg    10080

agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt    10140

gcggtatttc acaccgcaga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa    10200

taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa    10260

tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca    10320

gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca    10380

ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa    10440

gactatattc gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct    10500

cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt    10560

cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca    10620

cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg    10680

tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac    10740

tacgcggctg ctcaaacctg ggcagaacgt aagccgcgag agcgccaaca accgcttctt    10800

ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg    10860

agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa    10920

gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca    10980

tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc    11040

tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc    11100

tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc    11160

gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc    11220

atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc    11280

ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca    11340

tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg    11400

gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag    11460

atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt    11520

cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt    11580

tctagtggtt ggctacgtat actccggaat attaa                               11615


<210>  4
<211>  6126
<212>  DNA
<213>  Mus musculus

<400>  4
atggccacag atgacaagag ttccccgaca ctggactctg ctaatgattt gcctcgctct       60

cctgccagtc cttctcacct cactcacttt aaacccttga ctcctgacca ggatgagccc      120

cccttcaagt cagcatatag ttcttttgta aacctttttc gttttaacaa agagcgagga      180

gaagggggcc aaggagagca gcagtctccg agttcaagtt gggccagccc tcagatccct      240

tcaagaacac agtctgtgag gtcgcctgta ccttataaaa aacagcttaa tgaggagctc      300

caccggcgct cttcagtgtt agactccaga aggaaagcag aaccagcctg tggaggtcat      360

gacccacgta cagctgttca gcttcgaagc ctcagcacag tattgaaacg cctcaaagaa      420

atcatggaag gaaaaagcca ggacagtgac ctgaagcaat attggatgcc agatagccag      480

tgtaaagagt gctatgactg cagtgaaaag tttacaacat ttaggcgcag acaccattgc      540

agactgtgtg ggcagatttt ctgcagtcgt tgttgtaatc aagaaatccc tggaaaattt      600

atgggctata caggagacct ccgagcatgc acctactgta gaaaaatagc cttaagttat      660

gctcattcta cagacagtaa ttccattggg gaagacttga atgctctttc agattcaact      720

tgctctgtgt ctatacttga tccaagcgaa cctcggacac cagttgggag tagaaaagcc      780

agtcgtaaca tattcttaga ggatgattta gcctggcaaa gcttgattca tccggattcc      840

tcaaatagtg ctctttcaac aagactcgta tctgttcaag aggatgctgg gaagtctcct      900

gctcgaaaca gatcagccag cattactaat ctgtcactgg atcggtctgg ttctcctatg      960

gttccttcat atgagacatc tgtcagtccc caggctaacc gaaactacat taggacagag     1020

acgactgagg atgaacgcaa aattcttctg gacagtgctc agttaaagga tctgtggaag     1080

aaaatctgcc atcacaccag tgggatggaa tttcaagatc accgttactg gttgagaaca     1140

catcccaact gcattgtagg gaaggaatta gtcaactggc taatcagaaa tggacacatc     1200

gctacaaggg cacaagctat agcaattgga caagcaatgg ttgatggacg ttggttggat     1260

tgtgttagtc atcatgatca gcttttcagg gacgaatatg cgttgtatag accacttcag     1320

agtacagaat tttctgagac accttctcca gacagtgact ctgtgaactc tgtggaagga     1380

cactccgagc catcctggtt taaagacata aaatttgatg acagtgacac agaacagatt     1440

gctgaagaag gtgacgataa tttggctaag tatttggttt ctgacactgg aggacagcag     1500

ctctcaataa gtgatgcctt catcaaagag tccttattta atcgacgagt agaggaaaaa     1560

tccaaagagc tgccttttac ccctttgggc tggcatcata acaacctgga actcttgcga     1620

gaggagaatg aggagaagca agccatggaa aggctgcttt cagctaatca taaccacatg     1680

atggccctac tccagcagtt gcttcaaaac gagtcattgt catcgtcttg gagggacatc     1740

attgtgtcac tagtctgcca ggttgttcag acagtccgac ctgatgtcaa gcaccaggat     1800

gatgacatgg atatccgtca gtttgtccat atcaagaaga tcccaggtgg aaagaaattt     1860

gactctgtgg ttgtcaatgg ctttgtttgt accaagaaca ttgcacataa aaagatgaat     1920

tcctgtatta aaaaccccaa aatccttctg ttgaagtgtt ctattgagta tctctataga     1980

gaagaaacta agtttacctg cattgatcct attgtgcttc aggaaaggga attcttgaag     2040

aattatgttc aacgaatagt tgatgttcga cccacattgg ttcttgttga gaaaacagtg     2100

tctcggattg ctcaggacat gttactggaa catggcatta ctctggtcat taatgtaaag     2160

tcacaagttt tagaaagaat cagtcgaatg acccaaggtg atttagtggt gtccatggac     2220

cagctgctca ccaaacccca cttgggcact tgccacaaat tttatatgca gatatttcag     2280

ctgcctaatg aacaaaccaa aacactgatg ttttttgaag gctgtccaca gcatctaggc     2340

tgcacaatca agctcagagg aggctctgat tatgagctgg ctcgagttaa ggagatccta     2400

atatttatga tctgtgtagc ttatcattct cagctagaaa tctcttttct catggatgag     2460

ttcgctatgc ctccaacatt aatgcaaagc ccttcattcc atcttctgac ggagggacga     2520

ggtgaagagg gagcctctca ggagcaggtc agtggcagct cccttcctca ggatcctgag     2580

tgccctcgtg aggccctgtc ttctgaggat agcactttgt tggaatcaag gactgtgcta     2640

gagaagggtg aactagacaa taaaagtatt ccacaagctg ttgcctcttt gaagcatcaa     2700

gattatacca cccccacttg cccagcaggt attccctgtg ctctttttgc attggtacca     2760

gagtcattgt tgcctctcca tatggatcaa caggatgccg taggaaatga acagccagag     2820

acttcacagc aaacggatga gcaacaggat cccaaaggcc agatgaaagc ttttagcgac     2880

cctttacagg atgacactgg aatgtacgtt actgaggaag tcacctcctc tgaagatcaa     2940

cgaaagactt atgccttgac atttaaacag gagttaaaag atgtaatcct ctgtatctct     3000

ccagttatta cattccgtga acctttcctt ttaactgaaa aggggatgag atgctcaact     3060

cgagattatt ttccagagca gatttactgg tctcctcttc tcaacaaaga ggtgaaggaa     3120

atggagagca ggaggaagaa acagctgctc agggatctct ctggacttca gggcatgaat     3180

ggcagtgttc aggccaagtc tattcaagtc ttaccctcac atgagctagt gagcaccagg     3240

attgctgaac atctgggtga cagccagacc ttgggtagaa tgctagctga ttatcgagct     3300

agaggaggaa gaattcagtc aaaacatttg gacccctttg tccattcaaa agatgcatca     3360

tgtacttcag gtggcaaatc aggaaacaaa actgagagtg atgaagagag gggattgatt     3420

ccaagtgatg taatatggcc aacaaaggtg gactgtctga accctgctaa ccaccagagg     3480

ctctgtgtgc tcttcagcgg ctcttctgcc cagtccagca atgctcccag tgcttgtgtc     3540

agtccttgga ttgtaacaat ggagttttat ggaaagaatg accttacact ggggatattt     3600

ttagaaagat actgtttcag gtcttcttac cagtgtccga gcatgttctg tgacaccccc     3660

atggttcatc acattcgacg ctttgttcat ggccaaggct gtgtacagat aattctgaag     3720

gagttggatt ctccagtgcc tggatatcaa catacaattc tcacatattc ctggtgcaga     3780

atctgcaaac aagtaacacc agttgttgct ctttcaaatg aatcctggtc tatgtcattt     3840

gcaaagtacc ttgaacttcg attttatggc caccagtaca cacgcagagc caacgctgag     3900

ccctgcggtc actctatcca ccatgattat catcagtatt tctcttataa ccagatggtg     3960

gcatctttca gttactctcc tattcggctt cttgaagtat gtgttccact accaaaaata     4020

ttcattaagc gtcaagcccc actgaaggta tctcttcttc aggacctcaa agactttttt     4080

cagaaggttt cacaggtgta cctagctgtt gatgagagac ttgcatcctt gaaaacggat     4140

acatttagca aaactagaga ggaaaagatg gaagatatct ttgcacaaaa ggagatggaa     4200

gagggtgagt ttaagaactg gacagagaag atgcaagcaa ggctcatgtc ttcctctgtg     4260

gatacccctc agcaactgca gtccattttt gagtcactga ttgccaagaa gcaaagcctc     4320

tgtgaggtgc tccaggcgtg gaacagcagg ttgcaggacc tcttccagca ggaaaaaggt     4380

agaaagaggc cttcagttcc tcccagtcct gggagactga gacaaggtga agaaagcaag     4440

ataaatgcaa tggacacatc tccaaggaat atttctccag gacttcacaa tggagaaaaa     4500

gaggatcgct tcttgacaac cctgtccagc cagagctcta cgagctccac ccacctccag     4560

ctgcccactc ctcccgaggc cctggccgag caggtagtgg gagggccgac tgatctggat     4620

tcagccagtg gctctgaaga tgtatttgat ggtcatttgc tgggatccac agacagccag     4680

gtgaaggaaa agtcaaccat gaaagccatc tttgctaatt tgcttccagg aaacagctac     4740

aatcccattc catttccttt tgatccagat aaacactact taatgtatga acatgaacgg     4800

gtgcccattg ctgtctgtga gaaagagccc agctccatca ttgcttttgc actcagttgt     4860

aaagaatacc gcaatgcctt agaggaattg tccaaagcaa ctctgcggaa cagtgctgaa     4920

gaagggctcc cagccaatag tgctttagat aacagaccta agagtagcag ccctattaga     4980

ctacctgaaa tcagtggagg acagacaaac cgcacagtag aagcagaacc tcagccaacc     5040

aaaaaagctt caggaatgtt gtccttcttc agaggaacag cagggaagag ccctgatctg     5100

tcttcccaga agagggagac cttgcgaggg gcagacagtg cttactacca ggttgggcag     5160

gccggcaagg aggggttgga gagtcaaggc ctggagcctc aagatgaagt agatggagga     5220

gatacacaga agaaacaact cacaaatcct catgtggaac ttcaattttc tgatgctaat     5280

gccaagtttt actgtcggct gtactacgcg ggagagttcc acaagatgcg tgaagtgatt     5340

ctgggcagca gtgaggagga attcatccgt tccctttctc actcatctcc ctggcaggcc     5400

cggggaggca agtcaggagc tgctttctat gccaccgaag atgatagatt cattctgaag     5460

caaatgcctc gtttggaagt ccagtctttc cttgactttg caccacacta cttcaattat     5520

atcacaaatg ctgttcaaca aaagaggccc accgccttgg ctaaaattct tggagtttac     5580

agaattggtt ataagaactc tcagaacaac actgagaaga agttagatct ccttgtcatg     5640

gaaaatcttt tctatgggag aaagatggca caggtttttg atttgaaggg ttcacttagg     5700

aatcgaaatg taaaaactga cactgggaaa gagagctgtg atgtggttct gttggatgaa     5760

aacctcctaa agatggttcg agacaaccct ctctatattc gttcccattc caaatctgtg     5820

ctgagaacct ccatccacag cgacgcccat ttcctttcca gccacctcat tatagactat     5880

tctctgctgg ttgggcgaga tgacactagc aatgagcttg tggttggcat catagattac     5940

attcgaacat ttacatggga caaaaaactt gagatggttg tgaagtcaac aggaatttta     6000

ggaggacaag gtaaaatgcc aactgtggtc tctccagagt tgtataggac tagattttgt     6060

gaagcaatgg acaagtattt cttgatggtg ccagaccact ggacagggtt ggatctgaat     6120

tgctga                                                                6126


<210>  5
<211>  11194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector for the production of recombinant 6His-SumoStar PIKFyve

<400>  5
tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt       60

ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca      120

ccatcgggcg cggatctcgg tccgatgggt catcaccatc atcatcacgg gtccctgcag      180

gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact      240

cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc      300

actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc      360

ttaacgttct tgtacgacgg tattgaaatt caagctgatc agacccctga agatttggac      420

atggaggata acgatattat tgaggctcac agagaacaga ttggaggtca gtcgactatg      480

gccacagatg acaagagttc cccgacactg gactctgcta atgatttgcc tcgctctcct      540

gccagtcctt ctcacctcac tcactttaaa cccttgactc ctgaccagga tgagcccccc      600

ttcaagtcag catatagttc ttttgtaaac ctttttcgtt ttaacaaaga gcgaggagaa      660

gggggccaag gagagcagca gtctccgagt tcaagttggg ccagccctca gatcccttca      720

agaacacagt ctgtgaggtc gcctgtacct tataaaaaac agcttaatga ggagctccac      780

cggcgctctt cagtgttaga ctccagaagg aaagcagaac cagcctgtgg aggtcatgac      840

ccacgtacag ctgttcagct tcgaagcctc agcacagtat tgaaacgcct caaagaaatc      900

atggaaggaa aaagccagga cagtgacctg aagcaatatt ggatgccaga tagccagtgt      960

aaagagtgct atgactgcag tgaaaagttt acaacattta ggcgcagaca ccattgcaga     1020

ctgtgtgggc agattttctg cagtcgttgt tgtaatcaag aaatccctgg aaaatttatg     1080

ggctatacag gagacctccg agcatgcacc tactgtagaa aaatagcctt aagttatgct     1140

cattctacag acagtaattc cattggggaa gacttgaatg ctctttcaga ttcaacttgc     1200

tctgtgtcta tacttgatcc aagcgaacct cggacaccag ttgggagtag aaaagccagt     1260

cgtaacatat tcttagagga tgatttagcc tggcaaagct tgattcatcc ggattcctca     1320

aatagtgctc tttcaacaag actcgtatct gttcaagagg atgctgggaa gtctcctgct     1380

cgaaacagat cagccagcat tactaatctg tcactggatc ggtctggttc tcctatggtt     1440

ccttcatatg agacatctgt cagtccccag gctaaccgaa actacattag gacagagacg     1500

actgaggatg aacgcaaaat tcttctggac agtgctcagt taaaggatct gtggaagaaa     1560

atctgccatc acaccagtgg gatggaattt caagatcacc gttactggtt gagaacacat     1620

cccaactgca ttgtagggaa ggaattagtc aactggctaa tcagaaatgg acacatcgct     1680

acaagggcac aagctatagc aattggacaa gcaatggttg atggacgttg gttggattgt     1740

gttagtcatc atgatcagct tttcagggac gaatatgcgt tgtatagacc acttcagagt     1800

acagaatttt ctgagacacc ttctccagac agtgactctg tgaactctgt ggaaggacac     1860

tccgagccat cctggtttaa agacataaaa tttgatgaca gtgacacaga acagattgct     1920

gaagaaggtg acgataattt ggctaagtat ttggtttctg acactggagg acagcagctc     1980

tcaataagtg atgccttcat caaagagtcc ttatttaatc gacgagtaga ggaaaaatcc     2040

aaagagctgc cttttacccc tttgggctgg catcataaca acctggaact cttgcgagag     2100

gagaatgagg agaagcaagc catggaaagg ctgctttcag ctaatcataa ccacatgatg     2160

gccctactcc agcagttgct tcaaaacgag tcattgtcat cgtcttggag ggacatcatt     2220

gtgtcactag tctgccaggt tgttcagaca gtccgacctg atgtcaagca ccaggatgat     2280

gacatggata tccgtcagtt tgtccatatc aagaagatcc caggtggaaa gaaatttgac     2340

tctgtggttg tcaatggctt tgtttgtacc aagaacattg cacataaaaa gatgaattcc     2400

tgtattaaaa accccaaaat ccttctgttg aagtgttcta ttgagtatct ctatagagaa     2460

gaaactaagt ttacctgcat tgatcctatt gtgcttcagg aaagggaatt cttgaagaat     2520

tatgttcaac gaatagttga tgttcgaccc acattggttc ttgttgagaa aacagtgtct     2580

cggattgctc aggacatgtt actggaacat ggcattactc tggtcattaa tgtaaagtca     2640

caagttttag aaagaatcag tcgaatgacc caaggtgatt tagtggtgtc catggaccag     2700

ctgctcacca aaccccactt gggcacttgc cacaaatttt atatgcagat atttcagctg     2760

cctaatgaac aaaccaaaac actgatgttt tttgaaggct gtccacagca tctaggctgc     2820

acaatcaagc tcagaggagg ctctgattat gagctggctc gagttaagga gatcctaata     2880

tttatgatct gtgtagctta tcattctcag ctagaaatct cttttctcat ggatgagttc     2940

gctatgcctc caacattaat gcaaagccct tcattccatc ttctgacgga gggacgaggt     3000

gaagagggag cctctcagga gcaggtcagt ggcagctccc ttcctcagga tcctgagtgc     3060

cctcgtgagg ccctgtcttc tgaggatagc actttgttgg aatcaaggac tgtgctagag     3120

aagggtgaac tagacaataa aagtattcca caagctgttg cctctttgaa gcatcaagat     3180

tataccaccc ccacttgccc agcaggtatt ccctgtgctc tttttgcatt ggtaccagag     3240

tcattgttgc ctctccatat ggatcaacag gatgccgtag gaaatgaaca gccagagact     3300

tcacagcaaa cggatgagca acaggatccc aaaggccaga tgaaagcttt tagcgaccct     3360

ttacaggatg acactggaat gtacgttact gaggaagtca cctcctctga agatcaacga     3420

aagacttatg ccttgacatt taaacaggag ttaaaagatg taatcctctg tatctctcca     3480

gttattacat tccgtgaacc tttcctttta actgaaaagg ggatgagatg ctcaactcga     3540

gattattttc cagagcagat ttactggtct cctcttctca acaaagaggt gaaggaaatg     3600

gagagcagga ggaagaaaca gctgctcagg gatctctctg gacttcaggg catgaatggc     3660

agtgttcagg ccaagtctat tcaagtctta ccctcacatg agctagtgag caccaggatt     3720

gctgaacatc tgggtgacag ccagaccttg ggtagaatgc tagctgatta tcgagctaga     3780

ggaggaagaa ttcagtcaaa acatttggac ccctttgtcc attcaaaaga tgcatcatgt     3840

acttcaggtg gcaaatcagg aaacaaaact gagagtgatg aagagagggg attgattcca     3900

agtgatgtaa tatggccaac aaaggtggac tgtctgaacc ctgctaacca ccagaggctc     3960

tgtgtgctct tcagcggctc ttctgcccag tccagcaatg ctcccagtgc ttgtgtcagt     4020

ccttggattg taacaatgga gttttatgga aagaatgacc ttacactggg gatattttta     4080

gaaagatact gtttcaggtc ttcttaccag tgtccgagca tgttctgtga cacccccatg     4140

gttcatcaca ttcgacgctt tgttcatggc caaggctgtg tacagataat tctgaaggag     4200

ttggattctc cagtgcctgg atatcaacat acaattctca catattcctg gtgcagaatc     4260

tgcaaacaag taacaccagt tgttgctctt tcaaatgaat cctggtctat gtcatttgca     4320

aagtaccttg aacttcgatt ttatggccac cagtacacac gcagagccaa cgctgagccc     4380

tgcggtcact ctatccacca tgattatcat cagtatttct cttataacca gatggtggca     4440

tctttcagtt actctcctat tcggcttctt gaagtatgtg ttccactacc aaaaatattc     4500

attaagcgtc aagccccact gaaggtatct cttcttcagg acctcaaaga cttttttcag     4560

aaggtttcac aggtgtacct agctgttgat gagagacttg catccttgaa aacggataca     4620

tttagcaaaa ctagagagga aaagatggaa gatatctttg cacaaaagga gatggaagag     4680

ggtgagttta agaactggac agagaagatg caagcaaggc tcatgtcttc ctctgtggat     4740

acccctcagc aactgcagtc catttttgag tcactgattg ccaagaagca aagcctctgt     4800

gaggtgctcc aggcgtggaa cagcaggttg caggacctct tccagcagga aaaaggtaga     4860

aagaggcctt cagttcctcc cagtcctggg agactgagac aaggtgaaga aagcaagata     4920

aatgcaatgg acacatctcc aaggaatatt tctccaggac ttcacaatgg agaaaaagag     4980

gatcgcttct tgacaaccct gtccagccag agctctacga gctccaccca cctccagctg     5040

cccactcctc ccgaggccct ggccgagcag gtagtgggag ggccgactga tctggattca     5100

gccagtggct ctgaagatgt atttgatggt catttgctgg gatccacaga cagccaggtg     5160

aaggaaaagt caaccatgaa agccatcttt gctaatttgc ttccaggaaa cagctacaat     5220

cccattccat ttccttttga tccagataaa cactacttaa tgtatgaaca tgaacgggtg     5280

cccattgctg tctgtgagaa agagcccagc tccatcattg cttttgcact cagttgtaaa     5340

gaataccgca atgccttaga ggaattgtcc aaagcaactc tgcggaacag tgctgaagaa     5400

gggctcccag ccaatagtgc tttagataac agacctaaga gtagcagccc tattagacta     5460

cctgaaatca gtggaggaca gacaaaccgc acagtagaag cagaacctca gccaaccaaa     5520

aaagcttcag gaatgttgtc cttcttcaga ggaacagcag ggaagagccc tgatctgtct     5580

tcccagaaga gggagacctt gcgaggggca gacagtgctt actaccaggt tgggcaggcc     5640

ggcaaggagg ggttggagag tcaaggcctg gagcctcaag atgaagtaga tggaggagat     5700

acacagaaga aacaactcac aaatcctcat gtggaacttc aattttctga tgctaatgcc     5760

aagttttact gtcggctgta ctacgcggga gagttccaca agatgcgtga agtgattctg     5820

ggcagcagtg aggaggaatt catccgttcc ctttctcact catctccctg gcaggcccgg     5880

ggaggcaagt caggagctgc tttctatgcc accgaagatg atagattcat tctgaagcaa     5940

atgcctcgtt tggaagtcca gtctttcctt gactttgcac cacactactt caattatatc     6000

acaaatgctg ttcaacaaaa gaggcccacc gccttggcta aaattcttgg agtttacaga     6060

attggttata agaactctca gaacaacact gagaagaagt tagatctcct tgtcatggaa     6120

aatcttttct atgggagaaa gatggcacag gtttttgatt tgaagggttc acttaggaat     6180

cgaaatgtaa aaactgacac tgggaaagag agctgtgatg tggttctgtt ggatgaaaac     6240

ctcctaaaga tggttcgaga caaccctctc tatattcgtt cccattccaa atctgtgctg     6300

agaacctcca tccacagcga cgcccatttc ctttccagcc acctcattat agactattct     6360

ctgctggttg ggcgagatga cactagcaat gagcttgtgg ttggcatcat agattacatt     6420

cgaacattta catgggacaa aaaacttgag atggttgtga agtcaacagg aattttagga     6480

ggacaaggta aaatgccaac tgtggtctct ccagagttgt ataggactag attttgtgaa     6540

gcaatggaca agtatttctt gatggtgcca gaccactgga cagggttgga tctgaattgc     6600

tgaaagggca attctgcaga tatccagcac agtggcggcc gctcgagtct agaattcttt     6660

aattaaaagt actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct     6720

ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt     6780

gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc     6840

acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta     6900

tcttatcatg tctggatctg atcactgctt gagcctagga gatccgaacc agataagtga     6960

aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc     7020

cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc     7080

ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt     7140

ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct     7200

gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat     7260

atcaacgctt atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag     7320

cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc     7380

cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc     7440

tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa     7500

aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg     7560

ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac     7620

actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta     7680

ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac     7740

gtttacaatt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt     7800

tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca     7860

ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt     7920

ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga     7980

tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa     8040

gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct     8100

gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat     8160

acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga     8220

tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc     8280

caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat     8340

gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa     8400

cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac     8460

tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa     8520

agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc     8580

tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc     8640

ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag     8700

acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta     8760

ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa     8820

gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc     8880

gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat     8940

ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga     9000

gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt     9060

ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata     9120

cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac     9180

cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg     9240

ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg     9300

tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag     9360

cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct     9420

ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc     9480

aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt     9540

ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg     9600

tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga     9660

gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg     9720

cggtatttca caccgcagac cagccgcgta acctggcaaa atcggttacg gttgagtaat     9780

aaatggatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat     9840

agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag     9900

tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat     9960

tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catggtaaag    10020

actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc    10080

ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc    10140

ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac    10200

gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt    10260

ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact    10320

acgcggctgc tcaaacctgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg    10380

gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga    10440

gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag    10500

agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat    10560

acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct    10620

gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct    10680

gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg    10740

ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca    10800

tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct    10860

tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat    10920

ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg    10980

cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga    11040

tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc    11100

gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct agctatagtt    11160

ctagtggttg gctacgtata ctccggaata ttaa                                11194


<210>  6
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sense primer

<400>  6
tcggtccgat gggtcatcac catcat                                            26


<210>  7
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense primer

<400>  7
catagtcgac tgacctccaa tctgttctct gt                                     32


