                         SEQUENCE LISTING

<110>  Cargill Incorporated
       BioAmber S.A.S.
 
<120>  YEAST CELLS HAVING REDUCTIVE TCA PATHWAY FROM PYRUVATE TO 
       SUCCINATE AND OVEREXPRESSING AN EXOGENOUS NAD(P)+ 
       TRANSHYDROGENASE ENZYME

<130>  N00188WO01

<150>  US 61/675,788
<151>  2012-07-25

<160>  146   

<170>  PatentIn version 3.5

<210>  1
<211>  4910
<212>  DNA
<213>  Artificial

<220>
<223>  URA 3 gene disruption fragment


<220>
<221>  misc_feature
<222>  (1656)..(1656)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (4312)..(4312)
<223>  n is a, c, g, or t

<400>  1
ctcaaaacta tttaattagt taattgtata aactgtatgt cattataaac agggaaggtt       60

gacattgtct agcggcaatc attgtctcat ttggttcatt aactttggtt ctgttcttgg      120

aaacgggtac caactctctc agagtgcttc aaaaattttt cagcacattt ggttagacat      180

gaactttctc tgctggttaa ggattcagag gtgaagtctt gaacacaatc gttgaaacat      240

ctgtccacaa gagatgtgta tagcctcatg aaatcagcca tttgcttttg ttcaacgatc      300

ttttgaaatt gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt      360

atgttgtagt tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt      420

gaaatgaaaa tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt      480

gcacggacat gtttttaaag gaagtactct ataccagtta ttcttcaccc tgcagggtac      540

gtagcatgca ctcgcaagct gtgccatcgc ccaacggtta attataagaa atcaacatca      600

gccaacaact attttcgtcc ccctcttttc agtggtaacg agcaattaca ttagtaagag      660

actattttct tcagtgattt gtaatttttt ttcagtgatt tgtaattctt tctcgaaata      720

tgcgggctta acttatccgg acattcacta catgcaagga aaaacgagaa ccgcggagat      780

ttcctcagta agtaacaatg atgatctttt tacgcttcat catcactttc caaagttcta      840

agctataagt tcaagcctag atacgctgaa aaactcctga ccaacaatgt aaagaaaaca      900

attacaattg taaggttgaa aacatctaaa aatgaaatat tttattgtac atgcacaccc      960

tgatagtcat tctcttactt catccctgaa agacgtggct gtacaagagt tggaatcgca     1020

aggtcatgag gttaaagtta gtgatcttta tgctcaaaag tggaaggcct caatagaccg     1080

tgacgacttc gagcagcttt tcgcaagaag agaggttaaa aataccccaa gcttcttatg     1140

aagcgtatgc cagaggagca ttaacaaaag acgtaaatca ggaacaggaa aaacttattt     1200

gggcggactt tgtcattttg tcgtttccta tatggtggtc ttctatgccg gctagtcgac     1260

cccctcgacc ccctcgagcg atctcgagat ttgctgcaac ggcaacatca atgtccacgt     1320

ttacacacct acatttatat ctatatttat atttatattt atttatttat gctacttagc     1380

ttctatagtt agttaatgca ctcacgatat tcaaaattga cacccttcaa ctactcccta     1440

ctattgtcta ctactgtcta ctactcctct ttactatagc tgctcccaat aggctccacc     1500

aataggctct gtcaatacat tttgcgccgc cacctttcag gttgtgtcac tcctgaagga     1560

ccatattggg taatcgtgca atttctggaa gagagtgccg cgagaagtga ggcccccact     1620

gtaaatcctc gagggggcat ggagtatggg gcatgnagga tggaggatgg gggggggggg     1680

ggaaaatagg tagcgaaagg acccgctatc accccacccg gagaactcgt tgccgggaag     1740

tcatatttcg acactccggg gagtctataa aaggcgggtt ttgtcttttg ccagttgatg     1800

ttgctgagag gacttgtttg ccgtttcttc cgatttaaca gtatagaatc aaccactgtt     1860

aattatacac gttatactaa cacaacaaaa acaaaaacaa cgacaacaac aacaacaatg     1920

tttgctttct actttctcac cgcatgcacc actttgaagg gtgttttcgg agtttctccg     1980

agttacaatg gtcttggtct caccccacag atgggttggg acagctggaa tacgtttgcc     2040

tgcgatgtca gtgaacagct acttctagac actgctgata gaatttctga cttggggcta     2100

aaggatatgg gttacaagta tgtcatccta gatgactgtt ggtctagcgg cagggattcc     2160

gacggtttcc tcgttgcaga caagcacaaa tttcccaacg gtatgggcca tgttgcagac     2220

cacctgcata ataacagctt tcttttcggt atgtattcgt ctgctggtga gtacacctgt     2280

gctgggtacc ctgggtctct ggggcgtgag gaagaagatg ctcaattctt tgcaaataac     2340

cgcgttgact acttgaagta tgataattgt tacaataaag gtcaatttgg tacaccagac     2400

gtttcttacc accgttacaa ggccatgtca gatgctttga ataaaactgg taggcctatt     2460

ttctattctc tatgtaactg gggtcaggat ttgacatttt actggggctc tggtatcgcc     2520

aattcttgga gaatgagcgg agatattact gctgagttca cccgtccaga tagcagatgt     2580

ccctgtgacg gtgacgaata tgattgcaag tacgccggtt tccattgttc tattatgaat     2640

attcttaaca aggcagctcc aatggggcaa aatgcaggtg ttggtggttg gaacgatctg     2700

gacaatctag aggtcggagt cggtaatttg actgacgatg aggaaaaggc ccatttctct     2760

atgtgggcaa tggtaaagtc cccacttatc attggtgccg acgtgaatca cttaaaggca     2820

tcttcgtact cgatctacag tcaagcctct gtcatcgcaa ttaatcaaga tccaaagggt     2880

attccagcca caagagtctg gagatattat gtttcagaca ccgatgaata tggacaaggt     2940

gaaattcaaa tgtggagtgg tccgcttgac aatggtgacc aagtggttgc tttattgaat     3000

ggaggaagcg tagcaagacc aatgaacacg accttggaag agattttctt tgacagcaat     3060

ttgggttcaa aggaactgac atcgacttgg gatatttacg acttatgggc caacagagtt     3120

gacaactcta cggcgtctgc tatccttgaa cagaataagg cagccaccgg tattctctac     3180

aatgctacag agcagtctta taaagacggt ttgtctaaga atgatacaag actgtttggc     3240

cagaaaattg gtagtctttc tccaaatgct atacttaaca caactgttcc agctcatggt     3300

atcgccttct ataggttgag accctcggct taagctcaat gttgagcaaa gcaggacgag     3360

aaaaaaaaaa ataatgattg ttaagaagtt catgaaaaaa aaaaggaaaa atactcaaat     3420

acttataaca gagtgattaa ataataaacg gcagtatacc ctatcaggta ttgagatagt     3480

tttatttttg taggtatata atctgaagcc tttgaactat tttctcgtat atatcatgga     3540

gtatacattg cattagcaac attgcatact agtcactcgc aagctgtgcc atcgcccaac     3600

ggttaattat aagaaatcaa catcagccaa caactatttt cgtccccctc ttttcagtgg     3660

taacgagcaa ttacattagt aagagactat tttcttcagt gatttgtaat tttttttcag     3720

tgatttgtaa ttctttctcg aaatatgcgg gctwaamtaa tccggacatt cactacatgc     3780

aaggaaaaac gagaaccgcg gagatttcct cagtaagtaa caatgatgat ctttttacgc     3840

ttcatcatca ctttccaaag ttctaagcta taagttcaag cctagatacg ctgaaaaact     3900

cctgaccaac aatgtaaaga aaacaattac aattgtaagg ttgaaaacat ctaaaaatga     3960

aatattttat tgtacatgca caccctgata gtcattctct tacttcatcc ctgaaagacg     4020

tggctgtaca agagttggaa tcgcaaggtc atgaggttaa agttagtgat ctttatgctc     4080

aaaagtggaa ggcctcaata gaccgtgacg acwwmaaaaa amaaamrmaa gaagagaggt     4140

taaaaatacc ccaagcttct tatgaagcgt atgccagagg agcattaaca aaagacgtaa     4200

atcaggaaca ggaaaaactt atttgggcgg actttgtcat tttgtcgttt cctatatggt     4260

ggtcttctat gccggctagc ggccgggcaa caaagcctcc cagatttgat anattttcaa     4320

tttgtgcttt gaatcatgac ttccacctgt ttggtccgca agaacacgta aatgcgcaat     4380

ttgtttctcc cttctgctta aaaaccatgc acctttaata ttatctggaa agataaagaa     4440

cagaattgtt gcgtagaaac aagtagcaga gccgtaaatg agaaaaatat acttccaagc     4500

tggtaatttc ccctttatta gtccaataca gtgtccgaag accccaccaa gaataccagc     4560

aagggtgttg aaatataatg tagatcttag tggttgttct gatttcttcc accacattcc     4620

gctaataatc ataaaagacg gtaatattcc ggcttcaaat acgccaagaa aaaacctcac     4680

ggtaaccaaa ccaccaaagc tatgacatgc agccatgcac ataagtaagc cgccccaaat     4740

gaacaaacaa atagacacaa atttgccaat tctaactcgt ggcaacaaaa aaaaggatat     4800

gaactcacct aataaataac cgaaataaaa agtagaagca actgtggaaa attgagaacc     4860

atgtaaattt gtgtcttctt tcaatgtata aacagccgca atacctaggg                4910


<210>  2
<211>  3704
<212>  DNA
<213>  Artificial

<220>
<223>  PDC gene disruption fragment

<400>  2
cccccagttg ttgttgcaat taacaaattt gctaccgaca ccgagaagga aattgagacc       60

attagagaag aagccatcaa ggctggtgca tttgatgctg ttgagtcaga ccattggtca      120

caaggtggta agggtgcaat caagttagct gaggcaattg tacgtgctac cgaggaaaga      180

ccgttggaag aaagtcaacc tcctaactat ctttattcat tagatggttc gttagaagat      240

agactaagaa caattgccac caagatgtat ggagcaaaag atattgaact atctgagttg      300

gccaagaaac agattgaaga gtatgagagt caaggttttg gcaagctgcc tgtttgtatt      360

gcaaagacgc aatattctct ctcccatgat ccaacattga aaggtgttcc aaaggatttc      420

atcttcccaa tcagagaagt tagaataagt gcaggagcag gatatttata tgcactagct      480

gcaaagatca tgacaattcc aggtctatca acttatgccg gatttatgaa tgttgaagtc      540

aacgaagatg gtgagattga tggattgttt tagtttttat tataaaatta tatattattc      600

ttaattacat atcacccttc tatcagggaa gggagaaacg aaaatagaga gtgacctatc      660

caagctcggg ggtctaagtt ttaatggccc agggaatcat tacttttttt tctcaatcct      720

tgatggataa aagtattaca tacgtacagg attgtgtatt agtgtatttc gttatatgat      780

taaacaaagt ttatagattg taaagtagac gtaaagttta gtaattcatt ttaatgttca      840

ttttacattc agatggcggc cgcggatcca gatcccccgg ggcgttgaag atctattctc      900

cagcaattaa atttgtgaag aataactggt atagagtact tcctttaaaa acatgtccgt      960

gcaccaagaa aaaaaaaaag tttgaaaaat tgtatgtcga cgaatttcag cattttcatt     1020

tcaaggcgat attatgtttc actaaactca ggacaggaat atactaagaa taactacaac     1080

atacacacaa cataagccaa gatggatcaa cttaactacc aagaacaaca acaatttcaa     1140

aagatcgttg aacaaaagca aatggctgat ttcatgaggc tatctgcaga tacgcggaac     1200

aatcaatcga taatgatttg actgataaag aaaaccatac ttttgtttat gtttattagt     1260

tatcgctttg ctacattaaa aattcacata ctaaagcctt tgttaaacaa ctttttctaa     1320

atcttaagat tttactctat ctagtttttt tggttgtagg tgaacgtaaa gtacctcatt     1380

tatttttttt tttttgcttg tgtaattctt ttcatgctta tttaaactag tgtacatgta     1440

tcaaatcttt gtgtaagaat catttaaatc tgtttaaata agcattccaa ccagcttgtt     1500

ggtatctttt agcttgctct ataggatctc ttccttgacc gtacaaacct ctaccaacaa     1560

ttatgatatc cgttccagtc tttacaactt catcaacagt tctatattgt tgaccaagtg     1620

catcaccttt gtcatctaaa ccaacccctg gagtcataat gatccagtca aaaccttctt     1680

ctctaccgcc catatcgtgt tgcgcaataa aaccaatgac aaactcttta tcagatttag     1740

caatttctac tgttttttct gtatattcac catatgctaa agaacccttt gatgataact     1800

cagcaagcat tagcaaacct ctaggttcac tggttgtttc ttgggctgcc tccttcaagc     1860

cagaaacaat acctgcaccc gttacaccat gtgcattagt gatgtcagcc cattcggcaa     1920

tacggaagac accagattta tattgatttt taacagtgtt accaatatca gcaaattttc     1980

tatcttcaaa aatcataaaa ttatgtttct tggcaagctc cttcaaaggc aacacagttc     2040

cttcatacgt aaaatcagaa acaatatcga tgtgtgtttt aactagacag atgtaaggac     2100

caatagtgtc caaaatagag agaagctttt cagtttcagt aatatccaat gatgcacaaa     2160

ggttagactt cttttcctcc atgatggaga aaagtctcct agcaacaggg gaagtgtgtg     2220

attctgatct ttctttgtat gacgccatcc ttgacaaaca aactacttta ttaaagcgtt     2280

gaagatctat tctccagcaa ttaaatttgt gaagaataac tggtatagag tacttccttt     2340

aaaaacatgt ccgtgcacca agaaaaaaaa aaagtttgaa aaattgtatg tcgacgaatt     2400

tcagcatttt catttcaagg cgatattatg tttcactaaa ctcaggacag gaatatacta     2460

agaataacta caacatacac acaacataag ccaagatgga tcaacttaac taccaagaac     2520

aacaacaatt tcaaaagatc gttgaacaaa agcaaatggc tgatttcatg aggctatgaa     2580

ttcttttatt ataaaattat atattattct taattacata tcacccttct atcagggaag     2640

ggagaaacga aaatagagag tgacctatcc aagcttgggg gtctaagttt taatggccca     2700

gggaatcatt actttttttt ctcaatcctt gatggataaa agtattacat acgtacagga     2760

ttgtgtatta gtgtatttcg ttatatgatt aaacaaagtt tatagattgt aaagtagacg     2820

taaagtttag taattcattt taatgttcat tttacattca gatgttaatt aaggcctcga     2880

gggatccgcg gccgctattt ttgtgttttg ctgtgttttg ttttattttg ttttattggg     2940

aagaaaatat ataataatag aatattatat taacaaataa ttaaagaagc tcaactgtta     3000

ttagaataaa tgggttctcc gtgtcctttt tatacgcctt ctccgaaaag aaaaaaacca     3060

tcgtatcatt tgtagcccac gccacccgga aaaaccacca ttgtcctcag cagtccgcaa     3120

aaatatggat gcgctcaatc aatttccctc ccccgtcaat gccaaaagga taacgacaca     3180

ctattaagag cgcatcattt gtaaaagccg aggaaggggg atacgctgac cgagacgtct     3240

cgcctcactc tcggagctga gccgccctcc ttaagaaatt catgggaaga acacccttcg     3300

cggcttctga acggctcgcc ctcgtccatt ggtcacctca cagtggcaac taataaggac     3360

attatagcaa tagaaattaa aatggtgcac agaaatacaa taggatcgaa taggatagga     3420

tacaataaga tacggaatat tagactatac tgtgatacgg tacgctacga tacgctacga     3480

tacgatacga tagaggatac cacggatata acgtagtgtt atttttcatt attggggttt     3540

tttttctgtt tgaattttcc acgtcaagag tatcccatct gacaggaacc gatggactcg     3600

tcacagtacc tatcgcccga gttcaatcca tggacgctgc gggtgaagga tcttcgcccg     3660

ctgttggcaa gccatgggat cagggcgtcg ccaagggacg ggcc                      3704


<210>  3
<211>  6392
<212>  DNA
<213>  Artificial

<220>
<223>  PYC gene integration fragment

<400>  3
ctaagtagtg gtgttggtga actcaagatg gactctttag gtaattatat tcttgaatag       60

ttgtgtaaag cgaatatgca aatagatttg ttttataatt atgcatctct ttgaaagagg      120

tttagaggca aagttcttgc atacaatatt gtgattgttt taatgtcatt cttgattttc      180

ataaagagat taaaaaaaaa aaaaaaaaac ttataaaatt gagtagaacc atttatatat      240

aagacaaaga ttgtctgtat tagtcctcaa cacactaaac cttacatact tagggtaaat      300

ttgctaatag agtgatatgt tcatgagaac tccaacgaca acacaaccac ctatttgcac      360

aacaaacacc attgtcgcac gctgcgcgcc ctagaagtag aaagaaaggg aaatgacatt      420

aagagaatca taccccgtgc ccgtaacgcc gaaaaaatca caccccgtcc cccacacctt      480

aaaacctcaa ccgcttaaca ccgccacacc ctttctcttt ataaacgccg tttgcattac      540

tcattcttct tataaaccgc accccccaaa acgcggaata gcttcaaccc cccaatcaga      600

tatgagtttc ccgggaaacc cgcttttccc gacagcccca caaggggttg gtctataaaa      660

gaggacgttt tccccgtcat cgagattgaa gattcttaca ggcccattta ttcaaattgg      720

agttgattct tcttgtcttt actttctttc tctctttttc ttcctttttt aatattatct      780

tttgtcaagc ctggttccct aagttgaact ctcttttctt gtgatcctcc tatatagata      840

cgccttgcca aatgcggccg cgagtccatc ggttcctgtc agatgggata ctcttgacgt      900

ggaaaattca aacagaaaaa aaaccccaat aatgaaaaat aacactacgt tatatccgtg      960

gtatcctcta tcgtatcgta tcgtagcgta tcgtagcgta ccgtatcaca gtatagtcta     1020

atattccgta tcttattgta tcctatccta ttcgatccta ttgtatttca gtgcaccatt     1080

ttaatttcta ttgctataat gtccttatta gttgccactg tgaggtgacc aatggacgag     1140

ggcgagccgt tcagaagccg cgaagggtgt tcttcccatg aatttcttaa ggagggcggc     1200

tcagctccga gagtgaggcg agacgtctcg gtcagcgtat cccccttcct cggcttttac     1260

aaatgatgcg ctcttaatag tgtgtcgtta tccttttggc attgacgggg gagggaaatt     1320

gattgagcgc atccatattt ttgcggactg ctgaggacaa tggtggtttt tccgggtggc     1380

gtgggctaca aatgatacga tggttttttt cttttcggag aaggcgtata aaaaggacac     1440

ggagaaccca tttattctaa aaacagttga gcttctttaa ttattttttg atataatatt     1500

ctattattat atattttctt cccaataaaa caaaataaaa caaaacacag caaaacacaa     1560

aaattctaga taaaatgtca actgtggaag atcactcctc cctacataaa ttgagaaagg     1620

aatctgagat tctttccaat gcaaacaaaa tcttagtggc taatagaggt gaaattccaa     1680

ttagaatttt caggtcagcc catgaattgt caatgcatac tgtggcgatc tattcccatg     1740

aagatcggtt gtccatgcat aggttgaagg ccgacgaggc ttatgcaatc ggtaagactg     1800

gtcaatattc gccagttcaa gcttatctac aaattgacga aattatcaaa atagcaaagg     1860

aacatgatgt ttccatgatc catccaggtt atggtttctt atctgaaaac tccgaattcg     1920

caaagaaggt tgaagaatcc ggtatgattt gggttgggcc tcctgctgaa gttattgatt     1980

ctgttggtga caaggtttct gcaagaaatt tggcaattaa atgtgacgtt cctgttgttc     2040

ctggtaccga tggtccaatt gaagacattg aacaggctaa acagtttgtg gaacaatatg     2100

gttatcctgt cattataaag gctgcatttg gtggtggtgg tagaggtatg agagttgtta     2160

gagaaggtga tgatatagtt gatgctttcc aaagagcgtc atctgaagca aagtctgcct     2220

ttggtaatgg tacttgtttt attgaaagat ttttggataa gccaaaacat attgaggttc     2280

aattattggc tgataattat ggtaacacaa tccatctctt tgaaagagat tgttctgttc     2340

aaagaagaca tcaaaaggtt gttgaaattg cacctgccaa aactttacct gttgaagtta     2400

gaaatgctat attaaaggat gctgtaacgt tagctaaaac cgctaactat agaaatgctg     2460

gtactgcaga atttttagtt gattcccaaa acagacatta ttttattgaa attaatccaa     2520

gaattcaagt tgaacataca attactgaag aaatcacggg tgttgatatt gttgccgctc     2580

aaattcaaat tgctgcaggt gcatcattgg aacaattggg tctattacaa aacaaaatta     2640

caactagagg ttttgcaatt caatgtagaa ttacaaccga ggatcctgct aagaattttg     2700

ccccagatac aggtaaaatt gaggtttata gatctgcagg tggtaacggt gtcagattag     2760

atggtggtaa tgggtttgcc ggtgctgtta tatctcctca ttatgactcg atgttggtta     2820

aatgttcaac atctggttct aactatgaaa ttgccagaag aaagatgatt agagctttag     2880

ttgaatttag aatcagaggt gtcaagacca atattccttt cttattggca ttgctaactc     2940

atccagtttt catttcgggt gattgttgga caacttttat tgatgatacc ccttcgttat     3000

tcgaaatggt ttcttcaaag aatagagccc aaaaattatt ggcatatatt ggtgacttgt     3060

gtgtcaatgg ttcttcaatt aaaggtcaaa ttggtttccc taaattgaac aaggaagcag     3120

aaatcccaga tttgttggat ccaaatgatg aggttattga tgtttctaaa ccttctacca     3180

atggtctaag accgtatcta ttaaagtatg gaccagatgc gttttccaaa aaagttcgtg     3240

aattcgatgg ttgtatgatt atggatacca cctggagaga tgcacatcaa tcattattgg     3300

ctacaagagt tagaactatt gatttactga gaattgctcc aacgactagt catgccttac     3360

aaaatgcatt tgcattagaa tgttggggtg gcgcaacatt tgatgttgcg atgaggttcc     3420

tctatgaaga tccttgggag agattaagac aacttagaaa ggcagttcca aatattcctt     3480

tccaaatgtt attgagaggt gctaatggtg ttgcttattc gtcattacct gataatgcaa     3540

ttgatcattt tgttaagcaa gcaaaggata atggtgttga tattttcaga gtctttgatg     3600

ctttgaacga tttggaacaa ttgaaggttg gtgttgatgc tgtcaagaaa gccggaggtg     3660

ttgttgaagc tacagtttgt tactcaggtg atatgttaat tccaggtaaa aagtataact     3720

tggattatta tttagagact gttggaaaga ttgtggaaat gggtacccat attttaggta     3780

ttaaggatat ggctggcacg ttaaagccaa aggctgctaa gttgttgatt ggctcgatca     3840

gatcaaaata ccctgacttg gttatccatg tccataccca tgactctgct ggtaccggta     3900

tttcaactta tgttgcatgc gcattggcag gtgccgacat tgtcgattgt gcaatcaatt     3960

cgatgtctgg tttaacctct caaccttcaa tgagtgcttt tattgctgct ttagatggtg     4020

atatcgaaac tggtgttcca gaacattttg caagacaatt agatgcatac tgggcagaaa     4080

tgagattgtt atactcatgt ttcgaagccg acttgaaggg accagaccca gaagtttata     4140

aacatgaaat tccaggtgga cagttgacta acctaatctt ccaagcccaa caagttggtt     4200

tgggtgaaca atgggaagaa actaagaaga agtatgaaga tgctaacatg ttgttgggtg     4260

atattgtcaa ggttacccca acctccaagg ttgttggtga tttagcccaa tttatggttt     4320

ctaataaatt agaaaaagaa gatgttgaaa aacttgctaa tgaattagat ttcccagatt     4380

cagttcttga tttctttgaa ggattaatgg gtacaccata tggtggattc ccagagcctt     4440

tgagaacaaa tgtcatttcc ggcaagagaa gaaaattaaa gggtagacca ggtttagaat     4500

tagaaccttt caacctcgag gaaatcagag aaaatttggt ttccagattt ggtccaggta     4560

ttactgaatg tgatgttgca tcttataaca tgtatccaaa ggtttacgag caatatcgta     4620

aggtggttga aaaatatggt gatttatctg ttttaccaac aaaagcattt ttggctcctc     4680

caactattgg tgaagaagtt catgtggaaa ttgagcaagg taagactttg attattaagt     4740

tattagccat ttctgacttg tctaaatctc atggtacaag agaagtatac tttgaattga     4800

atggtgaaat gagaaaggtt acaattgaag ataaaacagc tgcaattgag actgttacaa     4860

gagcaaaggc tgacggacac aatccaaatg aagttggtgc gccaatggct ggtgtcgttg     4920

ttgaagttag agtgaagcat ggaacagaag ttaagaaggg tgatccatta gccgttttga     4980

gtgcaatgaa aatggaaatg gttatttctg ctcctgttag tggtagggtc ggtgaagttt     5040

ttgtcaacga aggcgattcc gttgatatgg gtgatttgct tgtgaaaatt gccaaagatg     5100

aagcgccagc agcttaatta attctgtctt tgattttctt atgttattca aaacatctgc     5160

cccaaaatct aacgattata tatattccta cgtataactg tatagctaat tattgattta     5220

tttgtacata aaaaccacat aaatgtaaaa gcaagaaaaa aaataactaa ggagaaggat     5280

caatatctca tttataatgc tcgccaaagc agcgtacgtg aattttaatc aagacatcaa     5340

caaatcttgc aacttggtta tatcgcttct tcacccactc acccgctttt ctacattgtt     5400

gaacacaaat atatacaggg gtatgtctca aggtcaagtg cagtttcaac agagactacc     5460

tcaaggtacc tcttcagaaa tgcagaactt cactcttgat cagattttct ccgaattaaa     5520

ggtttaaaca tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt     5580

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt     5640

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     5700

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     5760

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     5820

atcttcaacg ctttaataaa gtagtttgtt tgtcaaggat ggcgtcatac aaagaaagat     5880

cagaatcaca cacttcccct gttgctagga gacttttctc catcatggag gaaaagaagt     5940

ctaacctttg tgcatcattg gatattactg aaactgaaaa gcttctctct attttggaca     6000

ctattggtcc ttacatctgt ctagttaaaa cacacatcga tattgtttct gattttacgt     6060

atgaaggaac tgtgttgcct ttgaaggagc ttgccaagaa acataatttt atgatttttg     6120

aagatagaaa atttgctgat attggtaaca ctgttaaaaa tcaatataaa tctggtgtct     6180

tccgtattgc cgaatgggct gacatcacta atgcacatgg tgtaacgggt gcaggtattg     6240

tttctggctt gaaggaggca gcccaagaaa caaccagtga acctagaggt ttgctaatgc     6300

ttgctgagtt atcatcaaag ggttctttag catatggtga atatacagaa aaaacagtag     6360

aaattgctaa atctgataaa gagtttgttg ag                                   6392


<210>  4
<211>  3543
<212>  DNA
<213>  Issatchenkia orientalis

<400>  4
atgtcaactg tggaagatca ctcctcccta cataaattga gaaaggaatc tgagattctt       60

tccaatgcaa acaaaatctt agtggctaat agaggtgaaa ttccaattag aattttcagg      120

tcagcccatg aattgtcaat gcatactgtg gcgatctatt cccatgaaga tcggttgtcc      180

atgcataggt tgaaggccga cgaggcttat gcaatcggta agactggtca atattcgcca      240

gttcaagctt atctacaaat tgacgaaatt atcaaaatag caaaggaaca tgatgtttcc      300

atgatccatc caggttatgg tttcttatct gaaaactccg aattcgcaaa gaaggttgaa      360

gaatccggta tgatttgggt tgggcctcct gctgaagtta ttgattctgt tggtgacaag      420

gtttctgcaa gaaatttggc aattaaatgt gacgttcctg ttgttcctgg taccgatggt      480

ccaattgaag acattgaaca ggctaaacag tttgtggaac aatatggtta tcctgtcatt      540

ataaaggctg catttggtgg tggtggtaga ggtatgagag ttgttagaga aggtgatgat      600

atagttgatg ctttccaaag agcgtcatct gaagcaaagt ctgcctttgg taatggtact      660

tgttttattg aaagattttt ggataagcca aaacatattg aggttcaatt attggctgat      720

aattatggta acacaatcca tctctttgaa agagattgtt ctgttcaaag aagacatcaa      780

aaggttgttg aaattgcacc tgccaaaact ttacctgttg aagttagaaa tgctatatta      840

aaggatgctg taacgttagc taaaaccgct aactatagaa atgctggtac tgcagaattt      900

ttagttgatt cccaaaacag acattatttt attgaaatta atccaagaat tcaagttgaa      960

catacaatta ctgaagaaat cacgggtgtt gatattgttg ccgctcaaat tcaaattgct     1020

gcaggtgcat cattggaaca attgggtcta ttacaaaaca aaattacaac tagaggtttt     1080

gcaattcaat gtagaattac aaccgaggat cctgctaaga attttgcccc agatacaggt     1140

aaaattgagg tttatagatc tgcaggtggt aacggtgtca gattagatgg tggtaatggg     1200

tttgccggtg ctgttatatc tcctcattat gactcgatgt tggttaaatg ttcaacatct     1260

ggttctaact atgaaattgc cagaagaaag atgattagag ctttagttga atttagaatc     1320

agaggtgtca agaccaatat tcctttctta ttggcattgc taactcatcc agttttcatt     1380

tcgggtgatt gttggacaac ttttattgat gatacccctt cgttattcga aatggtttct     1440

tcaaagaata gagcccaaaa attattggca tatattggtg acttgtgtgt caatggttct     1500

tcaattaaag gtcaaattgg tttccctaaa ttgaacaagg aagcagaaat cccagatttg     1560

ttggatccaa atgatgaggt tattgatgtt tctaaacctt ctaccaatgg tctaagaccg     1620

tatctattaa agtatggacc agatgcgttt tccaaaaaag ttcgtgaatt cgatggttgt     1680

atgattatgg ataccacctg gagagatgca catcaatcat tattggctac aagagttaga     1740

actattgatt tactgagaat tgctccaacg actagtcatg ccttacaaaa tgcatttgca     1800

ttagaatgtt ggggtggcgc aacatttgat gttgcgatga ggttcctcta tgaagatcct     1860

tgggagagat taagacaact tagaaaggca gttccaaata ttcctttcca aatgttattg     1920

agaggtgcta atggtgttgc ttattcgtca ttacctgata atgcaattga tcattttgtt     1980

aagcaagcaa aggataatgg tgttgatatt ttcagagtct ttgatgcttt gaacgatttg     2040

gaacaattga aggttggtgt tgatgctgtc aagaaagccg gaggtgttgt tgaagctaca     2100

gtttgttact caggtgatat gttaattcca ggtaaaaagt ataacttgga ttattattta     2160

gagactgttg gaaagattgt ggaaatgggt acccatattt taggtattaa ggatatggct     2220

ggcacgttaa agccaaaggc tgctaagttg ttgattggct cgatcagatc aaaataccct     2280

gacttggtta tccatgtcca tacccatgac tctgctggta ccggtatttc aacttatgtt     2340

gcatgcgcat tggcaggtgc cgacattgtc gattgtgcaa tcaattcgat gtctggttta     2400

acctctcaac cttcaatgag tgcttttatt gctgctttag atggtgatat cgaaactggt     2460

gttccagaac attttgcaag acaattagat gcatactggg cagaaatgag attgttatac     2520

tcatgtttcg aagccgactt gaagggacca gacccagaag tttataaaca tgaaattcca     2580

ggtggacagt tgactaacct aatcttccaa gcccaacaag ttggtttggg tgaacaatgg     2640

gaagaaacta agaagaagta tgaagatgct aacatgttgt tgggtgatat tgtcaaggtt     2700

accccaacct ccaaggttgt tggtgattta gcccaattta tggtttctaa taaattagaa     2760

aaagaagatg ttgaaaaact tgctaatgaa ttagatttcc cagattcagt tcttgatttc     2820

tttgaaggat taatgggtac accatatggt ggattcccag agcctttgag aacaaatgtc     2880

atttccggca agagaagaaa attaaagggt agaccaggtt tagaattaga acctttcaac     2940

ctcgaggaaa tcagagaaaa tttggtttcc agatttggtc caggtattac tgaatgtgat     3000

gttgcatctt ataacatgta tccaaaggtt tacgagcaat atcgtaaggt ggttgaaaaa     3060

tatggtgatt tatctgtttt accaacaaaa gcatttttgg ctcctccaac tattggtgaa     3120

gaagttcatg tggaaattga gcaaggtaag actttgatta ttaagttatt agccatttct     3180

gacttgtcta aatctcatgg tacaagagaa gtatactttg aattgaatgg tgaaatgaga     3240

aaggttacaa ttgaagataa aacagctgca attgagactg ttacaagagc aaaggctgac     3300

ggacacaatc caaatgaagt tggtgcgcca atggctggtg tcgttgttga agttagagtg     3360

aagcatggaa cagaagttaa gaagggtgat ccattagccg ttttgagtgc aatgaaaatg     3420

gaaatggtta tttctgctcc tgttagtggt agggtcggtg aagtttttgt caacgaaggc     3480

gattccgttg atatgggtga tttgcttgtg aaaattgcca aagatgaagc gccagcagct     3540

taa                                                                   3543


<210>  5
<211>  4886
<212>  DNA
<213>  Artificial

<220>
<223>  MAE gene integration fragment

<400>  5
ctttgaagga gcttgccaag aaacataatt ttatgatttt tgaagataga aaatttgctg       60

atattggtaa cactgttaaa aatcaatata aatctggtgt cttccgtatt gccgaatggg      120

ctgacatcac taatgcacat ggtgtaacgg gtgcaggtat tgtttctggc ttgaaggagg      180

cagcccaaga aacaaccagt gaacctagag gtttgctaat gcttgctgag ttatcatcaa      240

agggttcttt agcatatggt gaatatacag aaaaaacagt agaaattgct aaatctgata      300

aagagtttgt cattggtttt attgcgcaac acgatatggg cggtagagaa gaaggttttg      360

actggatcat tatgactcca ggggttggtt tagatgacaa aggtgatgca cttggtcaac      420

aatatagaac tgttgatgaa gttgtaaaga ctggaacgga tatcataatt gttggtagag      480

gtttgtacgg tcaaggaaga gatcctatag agcaagctaa aagataccaa caagctggtt      540

ggaatgctta tttaaacaga tttaaatgat tcttacacaa agatttgata catgtacact      600

agtttaaata agcatgaaaa gaattacaca agcaaaaaaa aaaaaataaa tgaggtactt      660

tacgttcacc tacaaccaaa aaaactagat agagtaaaat cttaagattt agaaaaagtt      720

gtttaacaaa ggctttagta tgtgaatttt taatgtagca aagcgataac taataaacat      780

aaacaaaagt atggttttct ttatcagtca aatcattatc gattgattgt tccgcgtatc      840

tgcagatagc ctcatgaaat cagccatttg cttttgttca acgatctttt gaaattgttg      900

ttgttcttgg tagttaagtt gatccatctt ggcttatgtt gtgtgtatgt tgtagttatt      960

cttagtatat tcctgtcctg agtttagtga aacataatat cgccttgaaa tgaaaatgct     1020

gaaattcgtc gacatacaat ttttcaaact tttttttttt cttggtgcac ggacatgttt     1080

ttaaaggaag tactctatac cagttattct tcacaaattt aattgctgga gaatagatct     1140

tcaacgcgtt taaacagcaa tttgaggaag gaataggaga aggagaagca atttctagga     1200

aagagcaagg tgtgcaacag catgctctga atgatatttt cagcaatagt tcagttgaag     1260

aacctgttgg cgtatctaca tcacttccta caaacaacac cacgaattgc gtccgtggtg     1320

acgcaactac gaatggcatt gtcaatgcca atgccagtgc acatacacgt gcaagtccca     1380

ccggttccct gcccggctat ggtagagaca agaaggacga taccggcatc gacatcaaca     1440

gtttcaacag caatgcgttt ggcgtcgacg cgtcgatggg gctgccgtat ttggatttgg     1500

acgggctaga tttcgatatg gatatggata tggatatgga tatggagatg aatttgaatt     1560

tagatttggg tcttgatttg gggttggaat taaaagggga taacaatgag ggttttcctg     1620

ttgatttaaa caatggacgt gggaggtgat tgatttaacc tgatccaaaa ggggtatgtc     1680

tattttttag agtgtgtctt tgtgtcaaat tatggtagaa tgtgtaaagt agtataaact     1740

ttcctctcaa atgacgaggt ttaaaacacc ccccgggtga gccgagccga gaatggggca     1800

attgttcaat gtgaaataga agtatcgagt gagaaacttg ggtgttggcc agccaagggg     1860

gaaggaaaat ggcgcgaatg ctcaggtgag attgttttgg aattgggtga agcgaggaaa     1920

tgagcgaccc ggaggttgtg actttagtgg cggaggagga acgggaggaa aaggccaaga     1980

gggaaagtgt atataagggg gagcaatttg ccaaccagga tagaattgga tgagttataa     2040

ttctactgta tttattgtat aatttatttc tccttttata tcaaacacat tacaaaacac     2100

acaaaacata caaacataca cagctagcat gggtgaattg aaagagattt tgaaacaaag     2160

atatcatgaa ttacttgatt ggaatgttaa ggcaccacat gtccctttat cccagagatt     2220

gaagcacttt acttggtcat ggtttgcttg tactatggca accggtggtg ttggtttgat     2280

cattggttcc ttcccattca gattctacgg tttgaacacc attggcaaga ttgtttacat     2340

cttacaaatc tttttgtttt ctctttttgg ctcttgtatg ttgtttcgtt tcatcaagta     2400

tccatctacc attaaggact cttggaatca tcacttggaa aagttgttta tcgcaacttg     2460

tttgttatct atttccacat tcatcgacat gttagctatc tatgcttatc cagataccgg     2520

tgaatggatg gtctgggtca ttagaatctt atactacatc tatgtcgctg tctctttcat     2580

ctactgtgtt atggcctttt tcaccatttt caacaatcat gtttacacta ttgaaactgc     2640

ttctccagct tggattttgc caatcttccc tccaatgatc tgtggtgtca ttgctggtgc     2700

tgttaactcc acccaacctg ctcaccaatt gaaaaacatg gtcattttcg gtatcttgtt     2760

tcaaggttta ggtttttggg tttacctttt acttttcgcc gttaatgttt tgagattctt     2820

cacagtcggt ttagcaaagc cacaagatag accaggtatg tttatgttcg ttggtccacc     2880

agctttctct ggtttagcat tgattaacat tgcaagaggt gcaatgggct caagacctta     2940

cattttcgtt ggtgcaaact cttccgaata cttaggtttt gtctcaacct tcatggccat     3000

tttcatctgg ggtttagccg catggtgtta ttgcttagct atggtttcct tccttgccgg     3060

ctttttcact agagcaccat tgaaattcgc ttgtggttgg ttcgctttca tctttccaaa     3120

tgttggtttt gttaactgta ctatcgaaat cggcaagatg attgattcta aggcttttca     3180

aatgtttggt cacatcattg gtgttatctt gtgtattcaa tggattttgt taatgtactt     3240

aatggttaga gcattccttg ttaatgactt gtgctatcct ggtaaagacg aagatgcaca     3300

cccaccacca aagccaaaca ctggtgtctt aaacccaact ttcccaccag agaaggctcc     3360

agcatcatta gagaaggttg atactcatgt tacatcaaca ggtggtgaat ccgatcctcc     3420

atcttccgaa catgaatccg tttaaggcgc gccatctaat agtttaatca cagcttatag     3480

tctactatag ttttcttttt taaacattgt tgtattttgt cccccccctc taattgatga     3540

tgattatcct ataagaatcc aataaaacga tggaaactaa taccctctcc tttgtcatgt     3600

ggtctttagt atttcttgaa cattggctct gatttctcga ctttatagtc ctattaaaat     3660

cgctgttagt tctcgatcgt tgtatctcgt ttcttgtctc tttggtggat gattttgcgt     3720

gcgaacatgt ttttttccct ttctctcacc atcatcgtgt agttcttgtc accatccccc     3780

ccaccccttc cttctctcat tgattctata agagcttatc cacagaggtg cagtaacgag     3840

gtagtttaac cttcgagtgg atcaaaatgt cacacaggcc tgcggccgct accataatgt     3900

atgcgttgag cctcttgcac cttctttatt aggaaatcag ttgaaaaatt tccggattgt     3960

ctttattatt ggcccatttt tttttggtca cacctttatt tttgtacact tctcgggcaa     4020

agcaaaaact atagtaccgg ataggccttt ataaaactcc agtgtgtatg attttagttg     4080

gtgtgccatc tacacgttct cttagtttct ttatcatgtc acagaaagca agcatgcaaa     4140

cccttacaaa aaataacaac atacaaatgc ctaaacaact ggactataat gatggtgagt     4200

cagttacgaa aagagcaagt gggttaatac gatttcgtaa gggacagtct gaggaagact     4260

acaattttca aaaggagcag ttctggtcca cgggtccttt agtacagaat cacacatttg     4320

tgactgaatt tgttgaaaag tttattgaaa acacaattag tgaagattat tcaatcacag     4380

atagatcgaa aatagaacgt gaaacaatca tacacggatt ggagaagctg tattttcaaa     4440

gggaatatga gcgatgtcta aaagatgttc aactattgaa ggacaatatc gataagttca     4500

atcctaattt ggatcttaat gaaaagaatt tataatgagc tgaattatat ttcttggatg     4560

tgcatcaaaa agatccatga gagtaacgaa aagaaactgg gggaaatcta ataatttaca     4620

atttcaatat acacttctat atcctttaat gtaatggctt tataaataaa cacgaacttc     4680

tacagcaccg acgtttcttt ttcttaccag ctcctcttct tcttcttctt cttcttcttc     4740

ttcttcttct tcttcttctt cttcttcttc ttcttcttct ttcttaccat cattgccatt     4800

ttcctttttt cttatttgct cttgatcctc tgttttttca atttggacaa actcatctaa     4860

tacaccaaca cttttagggc ccccgc                                          4886


<210>  6
<211>  1317
<212>  DNA
<213>  Schizosaccharomyces pombe

<400>  6
atgggtgaat tgaaagagat tttgaaacaa agatatcatg aattacttga ttggaatgtt       60

aaggcaccac atgtcccttt atcccagaga ttgaagcact ttacttggtc atggtttgct      120

tgtactatgg caaccggtgg tgttggtttg atcattggtt ccttcccatt cagattctac      180

ggtttgaaca ccattggcaa gattgtttac atcttacaaa tctttttgtt ttctcttttt      240

ggctcttgta tgttgtttcg tttcatcaag tatccatcta ccattaagga ctcttggaat      300

catcacttgg aaaagttgtt tatcgcaact tgtttgttat ctatttccac attcatcgac      360

atgttagcta tctatgctta tccagatacc ggtgaatgga tggtctgggt cattagaatc      420

ttatactaca tctatgtcgc tgtctctttc atctactgtg ttatggcctt tttcaccatt      480

ttcaacaatc atgtttacac tattgaaact gcttctccag cttggatttt gccaatcttc      540

cctccaatga tctgtggtgt cattgctggt gctgttaact ccacccaacc tgctcaccaa      600

ttgaaaaaca tggtcatttt cggtatcttg tttcaaggtt taggtttttg ggtttacctt      660

ttacttttcg ccgttaatgt tttgagattc ttcacagtcg gtttagcaaa gccacaagat      720

agaccaggta tgtttatgtt cgttggtcca ccagctttct ctggtttagc attgattaac      780

attgcaagag gtgcaatggg ctcaagacct tacattttcg ttggtgcaaa ctcttccgaa      840

tacttaggtt ttgtctcaac cttcatggcc attttcatct ggggtttagc cgcatggtgt      900

tattgcttag ctatggtttc cttccttgcc ggctttttca ctagagcacc attgaaattc      960

gcttgtggtt ggttcgcttt catctttcca aatgttggtt ttgttaactg tactatcgaa     1020

atcggcaaga tgattgattc taaggctttt caaatgtttg gtcacatcat tggtgttatc     1080

ttgtgtattc aatggatttt gttaatgtac ttaatggtta gagcattcct tgttaatgac     1140

ttgtgctatc ctggtaaaga cgaagatgca cacccaccac caaagccaaa cactggtgtc     1200

ttaaacccaa ctttcccacc agagaaggct ccagcatcat tagagaaggt tgatactcat     1260

gttacatcaa caggtggtga atccgatcct ccatcttccg aacatgaatc cgtttaa        1317


<210>  7
<211>  6527
<212>  DNA
<213>  Artificial

<220>
<223>  PYC gene inserter fragment

<400>  7
ctaaaagtgt tggtgtatta gatgagtttg tccaaattga aaaaacagag gatcaagagc       60

aaataagaaa aaaggaaaat ggcaatgatg gtaagaaaga agaagaagaa gaagaagaag      120

aagaagaaga agaagaagaa gaagaagaag aagaagaaga agaggagctg gtaagaaaaa      180

gaaacgtcgg tgctgtagaa gttcgtgttt atttataaag ccattacatt aaaggatata      240

gaagtgtata ttgaaattgt aaattattag atttccccca gtttcttttc gttactctca      300

tggatctttt tgatgcacat ccaagaaata taattcagct cattataaat tcttttcatt      360

aagatccaaa ttaggattga acttatcgat attgtccttc aatagttgaa catcttttag      420

acatcgctca tattcccttt gaaaatacag cttctccaat ccgtgtatga ttgtttcacg      480

ttctattttc gatctatctg tgattgaata atcttcacta attgtgtttt caataaactt      540

ttcaacaaat tcagtcacaa atgtgtgatt ctgtactaaa ggacccgtgg accagaactg      600

ctccttttga aaattgtagt cttcctcaga ctgtccctta cgaaatcgta ttaacccact      660

tgctcttttc gtaactgact caccatcatt atagtccagt tgtttaggca tttgtatgtt      720

gttatttttt gtaagggttt gcatgcttgc tttctgtgac atgataaaga aactaagaga      780

acgtgtagat ggcacaccaa ctaaaatcat acacactgga gttttataaa ggcctatccg      840

gtactatagt ttttgctttg cccgagaagt gtacaaaaat aaaggtgtga ccaaaaaaaa      900

atgggccaat aataaagaca atccggaaat ttttcaactg atttcctaat aaagaaggtg      960

caagaggctc aacgcataca ttatggtagc ggccgcgagt ccatcggttc ctgtcagatg     1020

ggatactctt gacgtggaaa attcaaacag aaaaaaaacc ccaataatga aaaataacac     1080

tacgttatat ccgtggtatc ctctatcgta tcgtatcgta gcgtatcgta gcgtaccgta     1140

tcacagtata gtctaatatt ccgtatctta ttgtatccta tcctattcga tcctattgta     1200

tttcagtgca ccattttaat ttctattgct ataatgtcct tattagttgc cactgtgagg     1260

tgaccaatgg acgagggcga gccgttcaga agccgcgaag ggtgttcttc ccatgaattt     1320

cttaaggagg gcggctcagc tccgagagtg aggcgagacg tctcggtcag cgtatccccc     1380

ttcctcggct tttacaaatg atgcgctctt aatagtgtgt cgttatcctt ttggcattga     1440

cgggggaggg aaattgattg agcgcatcca tatttttgcg gactgctgag gacaatggtg     1500

gtttttccgg gtggcgtggg ctacaaatga tacgatggtt tttttctttt cggagaaggc     1560

gtataaaaag gacacggaga acccatttat tctaaaaaca gttgagcttc tttaattatt     1620

ttttgatata atattctatt attatatatt ttcttcccaa taaaacaaaa taaaacaaaa     1680

cacagcaaaa cacaaaaatt ctagataaaa tgtcaactgt ggaagatcac tcctccctac     1740

ataaattgag aaaggaatct gagattcttt ccaatgcaaa caaaatctta gtggctaata     1800

gaggtgaaat tccaattaga attttcaggt cagcccatga attgtcaatg catactgtgg     1860

cgatctattc ccatgaagat cggttgtcca tgcataggtt gaaggccgac gaggcttatg     1920

caatcggtaa gactggtcaa tattcgccag ttcaagctta tctacaaatt gacgaaatta     1980

tcaaaatagc aaaggaacat gatgtttcca tgatccatcc aggttatggt ttcttatctg     2040

aaaactccga attcgcaaag aaggttgaag aatccggtat gatttgggtt gggcctcctg     2100

ctgaagttat tgattctgtt ggtgacaagg tttctgcaag aaatttggca attaaatgtg     2160

acgttcctgt tgttcctggt accgatggtc caattgaaga cattgaacag gctaaacagt     2220

ttgtggaaca atatggttat cctgtcatta taaaggctgc atttggtggt ggtggtagag     2280

gtatgagagt tgttagagaa ggtgatgata tagttgatgc tttccaaaga gcgtcatctg     2340

aagcaaagtc tgcctttggt aatggtactt gttttattga aagatttttg gataagccaa     2400

aacatattga ggttcaatta ttggctgata attatggtaa cacaatccat ctctttgaaa     2460

gagattgttc tgttcaaaga agacatcaaa aggttgttga aattgcacct gccaaaactt     2520

tacctgttga agttagaaat gctatattaa aggatgctgt aacgttagct aaaaccgcta     2580

actatagaaa tgctggtact gcagaatttt tagttgattc ccaaaacaga cattatttta     2640

ttgaaattaa tccaagaatt caagttgaac atacaattac tgaagaaatc acgggtgttg     2700

atattgttgc cgctcaaatt caaattgctg caggtgcatc attggaacaa ttgggtctat     2760

tacaaaacaa aattacaact agaggttttg caattcaatg tagaattaca accgaggatc     2820

ctgctaagaa ttttgcccca gatacaggta aaattgaggt ttatagatct gcaggtggta     2880

acggtgtcag attagatggt ggtaatgggt ttgccggtgc tgttatatct cctcattatg     2940

actcgatgtt ggttaaatgt tcaacatctg gttctaacta tgaaattgcc agaagaaaga     3000

tgattagagc tttagttgaa tttagaatca gaggtgtcaa gaccaatatt cctttcttat     3060

tggcattgct aactcatcca gttttcattt cgggtgattg ttggacaact tttattgatg     3120

ataccccttc gttattcgaa atggtttctt caaagaatag agcccaaaaa ttattggcat     3180

atattggtga cttgtgtgtc aatggttctt caattaaagg tcaaattggt ttccctaaat     3240

tgaacaagga agcagaaatc ccagatttgt tggatccaaa tgatgaggtt attgatgttt     3300

ctaaaccttc taccaatggt ctaagaccgt atctattaaa gtatggacca gatgcgtttt     3360

ccaaaaaagt tcgtgaattc gatggttgta tgattatgga taccacctgg agagatgcac     3420

atcaatcatt attggctaca agagttagaa ctattgattt actgagaatt gctccaacga     3480

ctagtcatgc cttacaaaat gcatttgcat tagaatgttg gggtggcgca acatttgatg     3540

ttgcgatgag gttcctctat gaagatcctt gggagagatt aagacaactt agaaaggcag     3600

ttccaaatat tcctttccaa atgttattga gaggtgctaa tggtgttgct tattcgtcat     3660

tacctgataa tgcaattgat cattttgtta agcaagcaaa ggataatggt gttgatattt     3720

tcagagtctt tgatgctttg aacgatttgg aacaattgaa ggttggtgtt gatgctgtca     3780

agaaagccgg aggtgttgtt gaagctacag tttgttactc aggtgatatg ttaattccag     3840

gtaaaaagta taacttggat tattatttag agactgttgg aaagattgtg gaaatgggta     3900

cccatatttt aggtattaag gatatggctg gcacgttaaa gccaaaggct gctaagttgt     3960

tgattggctc gatcagatca aaataccctg acttggttat ccatgtccat acccatgact     4020

ctgctggtac cggtatttca acttatgttg catgcgcatt ggcaggtgcc gacattgtcg     4080

attgtgcaat caattcgatg tctggtttaa cctctcaacc ttcaatgagt gcttttattg     4140

ctgctttaga tggtgatatc gaaactggtg ttccagaaca ttttgcaaga caattagatg     4200

catactgggc agaaatgaga ttgttatact catgtttcga agccgacttg aagggaccag     4260

acccagaagt ttataaacat gaaattccag gtggacagtt gactaaccta atcttccaag     4320

cccaacaagt tggtttgggt gaacaatggg aagaaactaa gaagaagtat gaagatgcta     4380

acatgttgtt gggtgatatt gtcaaggtta ccccaacctc caaggttgtt ggtgatttag     4440

cccaatttat ggtttctaat aaattagaaa aagaagatgt tgaaaaactt gctaatgaat     4500

tagatttccc agattcagtt cttgatttct ttgaaggatt aatgggtaca ccatatggtg     4560

gattcccaga gcctttgaga acaaatgtca tttccggcaa gagaagaaaa ttaaagggta     4620

gaccaggttt agaattagaa cctttcaacc tcgaggaaat cagagaaaat ttggtttcca     4680

gatttggtcc aggtattact gaatgtgatg ttgcatctta taacatgtat ccaaaggttt     4740

acgagcaata tcgtaaggtg gttgaaaaat atggtgattt atctgtttta ccaacaaaag     4800

catttttggc tcctccaact attggtgaag aagttcatgt ggaaattgag caaggtaaga     4860

ctttgattat taagttatta gccatttctg acttgtctaa atctcatggt acaagagaag     4920

tatactttga attgaatggt gaaatgagaa aggttacaat tgaagataaa acagctgcaa     4980

ttgagactgt tacaagagca aaggctgacg gacacaatcc aaatgaagtt ggtgcgccaa     5040

tggctggtgt cgttgttgaa gttagagtga agcatggaac agaagttaag aagggtgatc     5100

cattagccgt tttgagtgca atgaaaatgg aaatggttat ttctgctcct gttagtggta     5160

gggtcggtga agtttttgtc aacgaaggcg attccgttga tatgggtgat ttgcttgtga     5220

aaattgccaa agatgaagcg ccagcagctt aattaattct gtctttgatt ttcttatgtt     5280

attcaaaaca tctgccccaa aatctaacga ttatatatat tcctacgtat aactgtatag     5340

ctaattattg atttatttgt acataaaaac cacataaatg taaaagcaag aaaaaaaata     5400

actaaggaga aggatcaata tctcatttat aatgctcgcc aaagcagcgt acgtgaattt     5460

taatcaagac atcaacaaat cttgcaactt ggttatatcg cttcttcacc cactcacccg     5520

cttttctaca ttgttgaaca caaatatata caggggtatg tctcaaggtc aagtgcagtt     5580

tcaacagaga ctacctcaag gtacctcttc agaaatgcag aacttcactc ttgatcagat     5640

tttctccgaa ttaaaggttt aaacatagcc tcatgaaatc agccatttgc ttttgttcaa     5700

cgatcttttg aaattgttgt tgttcttggt agttaagttg atccatcttg gcttatgttg     5760

tgtgtatgtt gtagttattc ttagtatatt cctgtcctga gtttagtgaa acataatatc     5820

gccttgaaat gaaaatgctg aaattcgtcg acatacaatt tttcaaactt tttttttttc     5880

ttggtgcacg gacatgtttt taaaggaagt actctatacc agttattctt cacaaattta     5940

attgctggag aatagatctt caacgcttta ataaagtagt ttgtttgtca aggatggcgt     6000

catacaaaga aagatcagaa tcacacactt cccctgttgc taggagactt ttctccatca     6060

tggaggaaaa gaagtctaac ctttgtgcat cattggatat tactgaaact gaaaagcttc     6120

tctctatttt ggacactatt ggtccttaca tctgtctagt taaaacacac atcgatattg     6180

tttctgattt tacgtatgaa ggaactgtgt tgcctttgaa ggagcttgcc aagaaacata     6240

attttatgat ttttgaagat agaaaatttg ctgatattgg taacactgtt aaaaatcaat     6300

ataaatctgg tgtcttccgt attgccgaat gggctgacat cactaatgca catggtgtaa     6360

cgggtgcagg tattgtttct ggcttgaagg aggcagccca agaaacaacc agtgaaccta     6420

gaggtttgct aatgcttgct gagttatcat caaagggttc tttagcatat ggtgaatata     6480

cagaaaaaac agtagaaatt gctaaatctg ataaagagtt tgttgag                   6527


<210>  8
<211>  4760
<212>  DNA
<213>  Artificial

<220>
<223>  MAE gene integration fragment

<400>  8
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttaaaca gcaatttgag gaaggaatag gagaaggaga agcaatttct     1200

aggaaagagc aaggtgtgca acagcatgct ctgaatgata ttttcagcaa tagttcagtt     1260

gaagaacctg ttggcgtatc tacatcactt cctacaaaca acaccacgaa ttgcgtccgt     1320

ggtgacgcaa ctacgaatgg cattgtcaat gccaatgcca gtgcacatac acgtgcaagt     1380

cccaccggtt ccctgcccgg ctatggtaga gacaagaagg acgataccgg catcgacatc     1440

aacagtttca acagcaatgc gtttggcgtc gacgcgtcga tggggctgcc gtatttggat     1500

ttggacgggc tagatttcga tatggatatg gatatggata tggatatgga gatgaatttg     1560

aatttagatt tgggtcttga tttggggttg gaattaaaag gggataacaa tgagggtttt     1620

cctgttgatt taaacaatgg acgtgggagg tgattgattt aacctgatcc aaaaggggta     1680

tgtctatttt ttagagtgtg tctttgtgtc aaattatggt agaatgtgta aagtagtata     1740

aactttcctc tcaaatgacg aggtttaaaa caccccccgg gtgagccgag ccgagaatgg     1800

ggcaattgtt caatgtgaaa tagaagtatc gagtgagaaa cttgggtgtt ggccagccaa     1860

gggggaagga aaatggcgcg aatgctcagg tgagattgtt ttggaattgg gtgaagcgag     1920

gaaatgagcg acccggaggt tgtgacttta gtggcggagg aggaacggga ggaaaaggcc     1980

aagagggaaa gtgtatataa gggggagcaa tttgccaacc aggatagaat tggatgagtt     2040

ataattctac tgtatttatt gtataattta tttctccttt tatatcaaac acattacaaa     2100

acacacaaaa catacaaaca tacacagcta gcatgggtga attgaaagag attttgaaac     2160

aaagatatca tgaattactt gattggaatg ttaaggcacc acatgtccct ttatcccaga     2220

gattgaagca ctttacttgg tcatggtttg cttgtactat ggcaaccggt ggtgttggtt     2280

tgatcattgg ttccttccca ttcagattct acggtttgaa caccattggc aagattgttt     2340

acatcttaca aatctttttg ttttctcttt ttggctcttg tatgttgttt cgtttcatca     2400

agtatccatc taccattaag gactcttgga atcatcactt ggaaaagttg tttatcgcaa     2460

cttgtttgtt atctatttcc acattcatcg acatgttagc tatctatgct tatccagata     2520

ccggtgaatg gatggtctgg gtcattagaa tcttatacta catctatgtc gctgtctctt     2580

tcatctactg tgttatggcc tttttcacca ttttcaacaa tcatgtttac actattgaaa     2640

ctgcttctcc agcttggatt ttgccaatct tccctccaat gatctgtggt gtcattgctg     2700

gtgctgttaa ctccacccaa cctgctcacc aattgaaaaa catggtcatt ttcggtatct     2760

tgtttcaagg tttaggtttt tgggtttacc ttttactttt cgccgttaat gttttgagat     2820

tcttcacagt cggtttagca aagccacaag atagaccagg tatgtttatg ttcgttggtc     2880

caccagcttt ctctggttta gcattgatta acattgcaag aggtgcaatg ggctcaagac     2940

cttacatttt cgttggtgca aactcttccg aatacttagg ttttgtctca accttcatgg     3000

ccattttcat ctggggttta gccgcatggt gttattgctt agctatggtt tccttccttg     3060

ccggcttttt cactagagca ccattgaaat tcgcttgtgg ttggttcgct ttcatctttc     3120

caaatgttgg ttttgttaac tgtactatcg aaatcggcaa gatgattgat tctaaggctt     3180

ttcaaatgtt tggtcacatc attggtgtta tcttgtgtat tcaatggatt ttgttaatgt     3240

acttaatggt tagagcattc cttgttaatg acttgtgcta tcctggtaaa gacgaagatg     3300

cacacccacc accaaagcca aacactggtg tcttaaaccc aactttccca ccagagaagg     3360

ctccagcatc attagagaag gttgatactc atgttacatc aacaggtggt gaatccgatc     3420

ctccatcttc cgaacatgaa tccgtttaag gcgcgccatc taatagttta atcacagctt     3480

atagtctact atagttttct tttttaaaca ttgttgtatt ttgtcccccc cctctaattg     3540

atgatgatta tcctataaga atccaataaa acgatggaaa ctaataccct ctcctttgtc     3600

atgtggtctt tagtatttct tgaacattgg ctctgatttc tcgactttat agtcctatta     3660

aaatcgctgt tagttctcga tcgttgtatc tcgtttcttg tctctttggt ggatgatttt     3720

gcgtgcgaac atgttttttt ccctttctct caccatcatc gtgtagttct tgtcaccatc     3780

ccccccaccc cttccttctc tcattgattc tataagagct tatccacaga ggtgcagtaa     3840

cgaggtagtt taaccttcga gtggatcaaa atgtcacaca ggcctgcggc cgcatttggc     3900

aaggcgtatc tatataggag gatcacaaga aaagagagtt caacttaggg aaccaggctt     3960

gacaaaagat aatattaaaa aaggaagaaa aagagagaaa gaaagtaaag acaagaagaa     4020

tcaactccaa tttgaataaa tgggcctgta agaatcttca atctcgatga cggggaaaac     4080

gtcctctttt atagaccaac cccttgtggg gctgtcggga aaagcgggtt tcccgggaaa     4140

ctcatatctg attggggggt tgaagctatt ccgcgttttg gggggtgcgg tttataagaa     4200

gaatgagtaa tgcaaacggc gtttataaag agaaagggtg tggcggtgtt aagcggttga     4260

ggttttaagg tgtgggggac ggggtgtgat tttttcggcg ttacgggcac ggggtatgat     4320

tctcttaatg tcatttccct ttctttctac ttctagggcg cgcagcgtgc gacaatggtg     4380

tttgttgtgc aaataggtgg ttgtgttgtc gttggagttc tcatgaacat atcactctat     4440

tagcaaattt accctaagta tgtaaggttt agtgtgttga ggactaatac agacaatctt     4500

tgtcttatat ataaatggtt ctactcaatt ttataagttt tttttttttt tttttaatct     4560

ctttatgaaa atcaagaatg acattaaaac aatcacaata ttgtatgcaa gaactttgcc     4620

tctaaacctc tttcaaagag atgcataatt ataaaacaaa tctatttgca tattcgcttt     4680

acacaactat tcaagaatat aattacctaa agagtccatc ttgagttcac caacaccact     4740

acttagagct cggtacccgc                                                 4760


<210>  9
<211>  6874
<212>  DNA
<213>  Artificial

<220>
<223>  FRD gene integration fragment

<400>  9
aaacctccgt tatgtatgtt tgtacccaaa aagaatgcgc tatattagtt taatctttta       60

taaacccgga attataaaaa tacagttagg aataaagtaa tagaaagatg aacaacgggc      120

ctaaaaagac taatgtgttg tggatcggaa tgtttcgaat agagtattaa agttatgctt      180

tcttttcttt ttgaacatgc ttggtattac tttgatatgc aaaagatatc gacaaattga      240

aaatggtttt gatgtctata gatgtggcat ggtaaggttc atttcaattt agcaaatatc      300

agacgagctc agcggccgcg gatccctcga ggagtccatc ggttcctgtc agatgggata      360

ctcttgacgt ggaaaattca aacagaaaaa aaaccccaat aatgaaaaat aacactacgt      420

tatatccgtg gtatcctcta tcgtatcgta tcgtagcgta tcgtagcgta ccgtatcaca      480

gtatagtcta atattccgta tcttattgta tcctatccta ttcgatccta ttgtatttca      540

gtgcaccatt ttaatttcta ttgctataat gtccttatta gttgccactg tgaggtgacc      600

aatggacgag ggcgagccgt tcagaagccg cgaagggtgt tcttcccatg aatttcttaa      660

ggagggcggc tcagctccga gagtgaggcg agacgtctcg gtcagcgtat cccccttcct      720

cggcttttac aaatgatgcg ctcttaatag tgtgtcgtta tccttttggc attgacgggg      780

gagggaaatt gattgagcgc atccatattt ttgcggactg ctgaggacaa tggtggtttt      840

tccgggtggc gtgggctaca aatgatacga tggttttttt cttttcggag aaggcgtata      900

aaaaggacac ggagaaccca tttattctaa aaacagttga gcttctttaa ttattttttg      960

atataatatt ctattattat atattttctt cccaataaaa caaaataaaa caaaacacag     1020

caaaacacaa aaattctaga atggctgatg gcaaaacctc tgcatcagtt gttgctgttg     1080

atgctgaacg tgccgctaag gaaagagatg cagcagctag agctatgttg caaggtggtg     1140

gtgtctctcc tgctggcaag gcacaattgt tgaaaaaggg tttggttcac actgttccat     1200

ataccttaaa ggttgtcgtc gcagatccaa aggaaatgga gaaggcaact gctgacgcag     1260

aagaggtttt acaagctgca tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa     1320

actcagaagt ttcaagagtc aataggttgg cagttggtga ggaacatcaa atgtctgaaa     1380

cattgaaaca cgtcatggcc tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg     1440

acccagcagt tggtccatta gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg     1500

ttccagccga aagagttaat gatttgttat ccaaatgtac ccttaatgca tctttttcaa     1560

ttgatatgtc cagaggtatg attgcaagga agcatccaga cgccatgttg gatttgggtg     1620

gtgtcaacaa gggttatggt atcgactaca ttgttgaaca cttaaactct ttgggttatg     1680

atgatgtctt tttcgaatgg ggtggtgatg ttagagcatc cggcaaaaac cagttatctc     1740

aaccttgggc tgttggtatt gttagaccac ctgccttggc cgacattaga actgttgtcc     1800

cagaggacaa aagatccttt atccgtgtcg tcagattgaa caacgaagct attgctacct     1860

ctggtgatta tgagaatttg gttgaaggtc ctggttctaa ggtttactct tccaccttca     1920

atccaacttc caaaaacttg ttggaaccta ccgaagcagg tatggctcaa gtttctgtca     1980

agtgttgctc atgtatctac gctgatgctt tagcaacagc agctttgttg aaaaacgatc     2040

ctgctgccgt tagaaggatc ttagataact ggagatatgt cagagatact gttactgact     2100

acaccactta cacaagggaa ggtgaaagag ttgctaagat gttggaaatt gctaccgaag     2160

atgctgaaat gagagcaaag agaatcaagg gctctttacc agcaagagtt atcattgttg     2220

gtggtggttt ggccggttgt tccgcagcta tcgaagcagc taactgtggc gcccacgtca     2280

tcttgttaga aaaggaacca aagttaggtg gtaactctgc aaaggctacc tccggtatca     2340

acgcctgggg tactagagca caagcaaaac aaggtgtcat ggacggcggc aagtttttcg     2400

aaagagatac ccatagatcc ggcaagggtg gtaattgcga tccatgcctt gttaagactt     2460

tgtccgttaa gtcctctgat gcagttaagt ggttatctga attaggtgtt ccattgactg     2520

ttttgtctca attaggtggt gcttcaagga aacgttgtca ccgtgcacca gataagtctg     2580

atggtacacc agtcccagtt ggtttcacca ttatgaaaac ccttgaaaac cacattgtca     2640

acgatttgtc cagacatgtt acagttatga caggtattac cgtcacagct ttagaatcta     2700

catcaagagt cagacctgat ggtgttttag tcaagcatgt tactggtgtt cacttgattc     2760

aggcatctgg tcaatctatg gttttgaatg cagacgctgt tatcttagct actggtggtt     2820

tctccaatga tcatacccca aactcccttt tacaacaata cgccccacag ttgtcatctt     2880

ttccaacaac caatggtgtc tgggcaactg gcgatggtgt taagatggct tccaagttgg     2940

gtgtcgcctt agttgatatg gataaggtcc aattacatcc taccggcttg ttagacccaa     3000

aagatccatc taatagaacc aagtatcttg gtccagaggc cttaagaggt tccggcggtg     3060

tcttgttaaa caaaaacggt gaaagatttg ttaatgaatt agacttaaga tctgttgtct     3120

ctcaagctat catcgcacaa gataatgagt acccaggctc tggtggttcc aagttcgcat     3180

actgtgtttt gaacgaaact gcagcaaagt tattcggcaa aaacttcctt ggtttctact     3240

ggaatagatt aggtcttttc caaaaggttg attccgttgc tggtttagct aagttgattg     3300

gttgtccaga agctaatgtt gttgctacat tgaagcaata tgaggagtta tcttccaaaa     3360

agcttaatcc ttgtccattg actggcaagt ctgtctttcc ttgtgtttta ggcactcaag     3420

gtccatacta tgttgccttg gttaccccat ccattcacta cactatgggt ggttgtttga     3480

tttccccatc tgctgagatg caaaccattg acaactctgg tgttactcct gtcagacgtc     3540

caatcttagg cttattcggt gctggtgaag ttactggcgg tgtccatggt ggtaacagat     3600

taggcggtaa ctctttgtta gaatgtgttg ttttcggcaa gatcgctggt gacagagctg     3660

caaccatctt gcaaaagaaa aacaccggct tatcaatgac agaatggtct actgtcgtct     3720

taagagaagt tagagaaggt ggtgtctatg gtgctggttc cagagttttg aggtttaaca     3780

tgcctggtgc attacagaga actggtttag ctttaggtca attcatcggt atcagaggtg     3840

attgggacgg tcacagattg atcggttact attctccaat cactttacct gatgatgttg     3900

gtgttattgg tatcttagct agagcagaca agggtagatt ggcagaatgg atttctgcat     3960

tgcagccagg tgacgctgtt gagatgaagg cctgcggtgg tcttatcatt gacagaagat     4020

tcgctgaaag acatttcttt ttccgtggtc ataagatcag aaagttggcc cttatcggtg     4080

gtggtactgg tgttgcacca atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg     4140

tcgattcaat tgagtccatt cagttcatct atgctgcaga ggatgtttcc gagcttacat     4200

acagaacctt acttgaatct tacgaagagg aatatggttc agaaaagttt aagtgtcact     4260

tcgttttgaa taacccacca gctcaatgga ctgacggtgt tggtttcgtt gatactgcat     4320

tgttgagatc cgcagttcaa gcaccatcaa atgatttgct tgttgcaatt tgtggtccac     4380

caatcatgca aagagcagtt aagggtgcat tgaaaggttt aggttacaat atgaatcttg     4440

ttagaaccgt tgacgaaact gaaccaccat cataattaat taacatctga atgtaaaatg     4500

aacattaaaa tgaattacta aactttacgt ctactttaca atctataaac tttgtttaat     4560

catataacga aatacactaa tacacaatcc tgtacgtatg taatactttt atccatcaag     4620

gattgagaaa aaaaagtaat gattccctgg gccattaaaa cttagacccc caagcttgga     4680

taggtcactc tctattttcg tttctccctt ccctgataga agggtgatat gtaattaaga     4740

ataatatata attttataat aaaagaattc atagcctcat gaaatcagcc atttgctttt     4800

gttcaacgat cttttgaaat tgttgttgtt cttggtagtt aagttgatcc atcttggctt     4860

atgttgtgtg tatgttgtag ttattcttag tatattcctg tcctgagttt agtgaaacat     4920

aatatcgcct tgaaatgaaa atgctgaaat tcgtcgacat acaatttttc aaactttttt     4980

tttttcttgg tgcacggaca tgtttttaaa ggaagtactc tataccagtt attcttcaca     5040

aatttaattg ctggagaata gatcttcaac gctttaataa agtagtttgt ttgtcaagga     5100

tggcgtcata caaagaaaga tcagaatcac acacttcccc tgttgctagg agacttttct     5160

ccatcatgga ggaaaagaag tctaaccttt gtgcatcatt ggatattact gaaactgaaa     5220

agcttctctc tattttggac actattggtc cttacatctg tctagttaaa acacacatcg     5280

atattgtttc tgattttacg tatgaaggaa ctgtgttgcc tttgaaggag cttgccaaga     5340

aacataattt tatgattttt gaagatagaa aatttgctga tattggtaac actgttaaaa     5400

atcaatataa atctggtgtc ttccgtattg ccgaatgggc tgacatcact aatgcacatg     5460

gtgtaacggg tgcaggtatt gtttctggct tgaaggaggc agcccaagaa acaaccagtg     5520

aacctagagg tttgctaatg cttgctgagt tatcatcaaa gggttcttta gcatatggtg     5580

aatatacaga aaaaacagta gaaattgcta aatctgataa agagtttgtc attggtttta     5640

ttgcgcaaca cgatatgggc ggtagagaag aaggttttga ctggatcatt atgactccag     5700

gggttggttt agatgacaaa ggtgatgcac ttggtcaaca atatagaact gttgatgaag     5760

ttgtaaagac tggaacggat atcataattg ttggtagagg tttgtacggt caaggaagag     5820

atcctataga gcaagctaaa agataccaac aagctggttg gaatgcttat ttaaacagat     5880

ttaaatgatt cttacacaaa gatttgatac atgtacacta gtttaaataa gcatgaaaag     5940

aattacacaa gcaaaaaaaa aaaaataaat gaggtacttt acgttcacct acaaccaaaa     6000

aaactagata gagtaaaatc ttaagattta gaaaaagttg tttaacaaag gctttagtat     6060

gtgaattttt aatgtagcaa agcgataact aataaacata aacaaaagta tggttttctt     6120

tatcagtcaa atcattatcg attgattgtt ccgcgtatct gcagatagcc tcatgaaatc     6180

agccatttgc ttttgttcaa cgatcttttg aaattgttgt tgttcttggt agttaagttg     6240

atccatcttg gcttatgttg tgtgtatgtt gtagttattc ttagtatatt cctgtcctga     6300

gtttagtgaa acataatatc gccttgaaat gaaaatgctg aaattcgtcg acatacaatt     6360

tttcaaactt tttttttttc ttggtgcacg gacatgtttt taaaggaagt actctatacc     6420

agttattctt cacaaattta attgctggag aatagatctt caacgccccg ggggatctgg     6480

atccgcggcc gctcatatgt ttgaaggtat tatcactgct gttgatttac gttcttgaaa     6540

actgcacgga taatattcac aatactaaca ataaagaaga ctcattgtgg aaggtgactc     6600

aatcatgcta gaaaagctgg ggaataaagg cacttttata gtagccacat tttggttcaa     6660

aagaatataa aggaaaaaaa aatattttcc agtgaaaaag aaaagactct ttctccgaga     6720

agccgagttt ctacgaggcc ttgttgagtc ataggggacc tctgtggttg actccggctt     6780

attacgtgaa tcatcggggg agccgcaccg tttgtccgcg acaggagaaa acgcaaggag     6840

tcaaacatta aattggtagg cactaccgag gttt                                 6874


<210>  10
<211>  3435
<212>  DNA
<213>  Leishmania mexicana

<400>  10
atggctgatg gcaaaacctc tgcatcagtt gttgctgttg atgctgaacg tgccgctaag       60

gaaagagatg cagcagctag agctatgttg caaggtggtg gtgtctctcc tgctggcaag      120

gcacaattgt tgaaaaaggg tttggttcac actgttccat ataccttaaa ggttgtcgtc      180

gcagatccaa aggaaatgga gaaggcaact gctgacgcag aagaggtttt acaagctgca      240

tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa actcagaagt ttcaagagtc      300

aataggttgg cagttggtga ggaacatcaa atgtctgaaa cattgaaaca cgtcatggcc      360

tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg acccagcagt tggtccatta      420

gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg ttccagccga aagagttaat      480

gatttgttat ccaaatgtac ccttaatgca tctttttcaa ttgatatgtc cagaggtatg      540

attgcaagga agcatccaga cgccatgttg gatttgggtg gtgtcaacaa gggttatggt      600

atcgactaca ttgttgaaca cttaaactct ttgggttatg atgatgtctt tttcgaatgg      660

ggtggtgatg ttagagcatc cggcaaaaac cagttatctc aaccttgggc tgttggtatt      720

gttagaccac ctgccttggc cgacattaga actgttgtcc cagaggacaa aagatccttt      780

atccgtgtcg tcagattgaa caacgaagct attgctacct ctggtgatta tgagaatttg      840

gttgaaggtc ctggttctaa ggtttactct tccaccttca atccaacttc caaaaacttg      900

ttggaaccta ccgaagcagg tatggctcaa gtttctgtca agtgttgctc atgtatctac      960

gctgatgctt tagcaacagc agctttgttg aaaaacgatc ctgctgccgt tagaaggatc     1020

ttagataact ggagatatgt cagagatact gttactgact acaccactta cacaagggaa     1080

ggtgaaagag ttgctaagat gttggaaatt gctaccgaag atgctgaaat gagagcaaag     1140

agaatcaagg gctctttacc agcaagagtt atcattgttg gtggtggttt ggccggttgt     1200

tccgcagcta tcgaagcagc taactgtggc gcccacgtca tcttgttaga aaaggaacca     1260

aagttaggtg gtaactctgc aaaggctacc tccggtatca acgcctgggg tactagagca     1320

caagcaaaac aaggtgtcat ggacggcggc aagtttttcg aaagagatac ccatagatcc     1380

ggcaagggtg gtaattgcga tccatgcctt gttaagactt tgtccgttaa gtcctctgat     1440

gcagttaagt ggttatctga attaggtgtt ccattgactg ttttgtctca attaggtggt     1500

gcttcaagga aacgttgtca ccgtgcacca gataagtctg atggtacacc agtcccagtt     1560

ggtttcacca ttatgaaaac ccttgaaaac cacattgtca acgatttgtc cagacatgtt     1620

acagttatga caggtattac cgtcacagct ttagaatcta catcaagagt cagacctgat     1680

ggtgttttag tcaagcatgt tactggtgtt cacttgattc aggcatctgg tcaatctatg     1740

gttttgaatg cagacgctgt tatcttagct actggtggtt tctccaatga tcatacccca     1800

aactcccttt tacaacaata cgccccacag ttgtcatctt ttccaacaac caatggtgtc     1860

tgggcaactg gcgatggtgt taagatggct tccaagttgg gtgtcgcctt agttgatatg     1920

gataaggtcc aattacatcc taccggcttg ttagacccaa aagatccatc taatagaacc     1980

aagtatcttg gtccagaggc cttaagaggt tccggcggtg tcttgttaaa caaaaacggt     2040

gaaagatttg ttaatgaatt agacttaaga tctgttgtct ctcaagctat catcgcacaa     2100

gataatgagt acccaggctc tggtggttcc aagttcgcat actgtgtttt gaacgaaact     2160

gcagcaaagt tattcggcaa aaacttcctt ggtttctact ggaatagatt aggtcttttc     2220

caaaaggttg attccgttgc tggtttagct aagttgattg gttgtccaga agctaatgtt     2280

gttgctacat tgaagcaata tgaggagtta tcttccaaaa agcttaatcc ttgtccattg     2340

actggcaagt ctgtctttcc ttgtgtttta ggcactcaag gtccatacta tgttgccttg     2400

gttaccccat ccattcacta cactatgggt ggttgtttga tttccccatc tgctgagatg     2460

caaaccattg acaactctgg tgttactcct gtcagacgtc caatcttagg cttattcggt     2520

gctggtgaag ttactggcgg tgtccatggt ggtaacagat taggcggtaa ctctttgtta     2580

gaatgtgttg ttttcggcaa gatcgctggt gacagagctg caaccatctt gcaaaagaaa     2640

aacaccggct tatcaatgac agaatggtct actgtcgtct taagagaagt tagagaaggt     2700

ggtgtctatg gtgctggttc cagagttttg aggtttaaca tgcctggtgc attacagaga     2760

actggtttag ctttaggtca attcatcggt atcagaggtg attgggacgg tcacagattg     2820

atcggttact attctccaat cactttacct gatgatgttg gtgttattgg tatcttagct     2880

agagcagaca agggtagatt ggcagaatgg atttctgcat tgcagccagg tgacgctgtt     2940

gagatgaagg cctgcggtgg tcttatcatt gacagaagat tcgctgaaag acatttcttt     3000

ttccgtggtc ataagatcag aaagttggcc cttatcggtg gtggtactgg tgttgcacca     3060

atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg tcgattcaat tgagtccatt     3120

cagttcatct atgctgcaga ggatgtttcc gagcttacat acagaacctt acttgaatct     3180

tacgaagagg aatatggttc agaaaagttt aagtgtcact tcgttttgaa taacccacca     3240

gctcaatgga ctgacggtgt tggtttcgtt gatactgcat tgttgagatc cgcagttcaa     3300

gcaccatcaa atgatttgct tgttgcaatt tgtggtccac caatcatgca aagagcagtt     3360

aagggtgcat tgaaaggttt aggttacaat atgaatcttg ttagaaccgt tgacgaaact     3420

gaaccaccat cataa                                                      3435


<210>  11
<211>  6874
<212>  DNA
<213>  Artificial

<220>
<223>  FRD gene integration fragment

<400>  11
aaacctcggt agtgcctacc aatttaatgt ttgactcctt gcgttttctc ctgtcgcgga       60

caaacggtgc ggctcccccg atgattcacg taataagccg gagtcaacca cagaggtccc      120

ctatgactca acaaggcctc gtagaaactc ggcttctcgg agaaagagtc ttttcttttt      180

cactggaaaa tatttttttt tcctttatat tcttttgaac caaaatgtgg ctactataaa      240

agtgccttta ttccccagct tttctagcat gattgagtca ccttccacaa tgagtcttct      300

ttattgttag tattgtgaat attatccgtg cagttttcaa gaacgtaaat caacagcagt      360

gataatacct tcaaacatat gagcggccgc ggatccctcg aggagtccat cggttcctgt      420

cagatgggat actcttgacg tggaaaattc aaacagaaaa aaaaccccaa taatgaaaaa      480

taacactacg ttatatccgt ggtatcctct atcgtatcgt atcgtagcgt atcgtagcgt      540

accgtatcac agtatagtct aatattccgt atcttattgt atcctatcct attcgatcct      600

attgtatttc agtgcaccat tttaatttct attgctataa tgtccttatt agttgccact      660

gtgaggtgac caatggacga gggcgagccg ttcagaagcc gcgaagggtg ttcttcccat      720

gaatttctta aggagggcgg ctcagctccg agagtgaggc gagacgtctc ggtcagcgta      780

tcccccttcc tcggctttta caaatgatgc gctcttaata gtgtgtcgtt atccttttgg      840

cattgacggg ggagggaaat tgattgagcg catccatatt tttgcggact gctgaggaca      900

atggtggttt ttccgggtgg cgtgggctac aaatgatacg atggtttttt tcttttcgga      960

gaaggcgtat aaaaaggaca cggagaaccc atttattcta aaaacagttg agcttcttta     1020

attatttttt gatataatat tctattatta tatattttct tcccaataaa acaaaataaa     1080

acaaaacaca gcaaaacaca aaaattctag aatggctgat ggcaaaacct ctgcatcagt     1140

tgttgctgtt gatgctgaac gtgccgctaa ggaaagagat gcagcagcta gagctatgtt     1200

gcaaggtggt ggtgtctctc ctgctggcaa ggcacaattg ttgaaaaagg gtttggttca     1260

cactgttcca tataccttaa aggttgtcgt cgcagatcca aaggaaatgg agaaggcaac     1320

tgctgacgca gaagaggttt tacaagctgc atttcaagtc gtcgacaccc ttttgaacaa     1380

ctttaacgaa aactcagaag tttcaagagt caataggttg gcagttggtg aggaacatca     1440

aatgtctgaa acattgaaac acgtcatggc ctgttgtcaa aaggtttatc attcctccag     1500

aggtgttttt gacccagcag ttggtccatt agtccgtgaa cttagagaag ctgctcacaa     1560

gggtaaaact gttccagccg aaagagttaa tgatttgtta tccaaatgta cccttaatgc     1620

atctttttca attgatatgt ccagaggtat gattgcaagg aagcatccag acgccatgtt     1680

ggatttgggt ggtgtcaaca agggttatgg tatcgactac attgttgaac acttaaactc     1740

tttgggttat gatgatgtct ttttcgaatg gggtggtgat gttagagcat ccggcaaaaa     1800

ccagttatct caaccttggg ctgttggtat tgttagacca cctgccttgg ccgacattag     1860

aactgttgtc ccagaggaca aaagatcctt tatccgtgtc gtcagattga acaacgaagc     1920

tattgctacc tctggtgatt atgagaattt ggttgaaggt cctggttcta aggtttactc     1980

ttccaccttc aatccaactt ccaaaaactt gttggaacct accgaagcag gtatggctca     2040

agtttctgtc aagtgttgct catgtatcta cgctgatgct ttagcaacag cagctttgtt     2100

gaaaaacgat cctgctgccg ttagaaggat cttagataac tggagatatg tcagagatac     2160

tgttactgac tacaccactt acacaaggga aggtgaaaga gttgctaaga tgttggaaat     2220

tgctaccgaa gatgctgaaa tgagagcaaa gagaatcaag ggctctttac cagcaagagt     2280

tatcattgtt ggtggtggtt tggccggttg ttccgcagct atcgaagcag ctaactgtgg     2340

cgcccacgtc atcttgttag aaaaggaacc aaagttaggt ggtaactctg caaaggctac     2400

ctccggtatc aacgcctggg gtactagagc acaagcaaaa caaggtgtca tggacggcgg     2460

caagtttttc gaaagagata cccatagatc cggcaagggt ggtaattgcg atccatgcct     2520

tgttaagact ttgtccgtta agtcctctga tgcagttaag tggttatctg aattaggtgt     2580

tccattgact gttttgtctc aattaggtgg tgcttcaagg aaacgttgtc accgtgcacc     2640

agataagtct gatggtacac cagtcccagt tggtttcacc attatgaaaa cccttgaaaa     2700

ccacattgtc aacgatttgt ccagacatgt tacagttatg acaggtatta ccgtcacagc     2760

tttagaatct acatcaagag tcagacctga tggtgtttta gtcaagcatg ttactggtgt     2820

tcacttgatt caggcatctg gtcaatctat ggttttgaat gcagacgctg ttatcttagc     2880

tactggtggt ttctccaatg atcatacccc aaactccctt ttacaacaat acgccccaca     2940

gttgtcatct tttccaacaa ccaatggtgt ctgggcaact ggcgatggtg ttaagatggc     3000

ttccaagttg ggtgtcgcct tagttgatat ggataaggtc caattacatc ctaccggctt     3060

gttagaccca aaagatccat ctaatagaac caagtatctt ggtccagagg ccttaagagg     3120

ttccggcggt gtcttgttaa acaaaaacgg tgaaagattt gttaatgaat tagacttaag     3180

atctgttgtc tctcaagcta tcatcgcaca agataatgag tacccaggct ctggtggttc     3240

caagttcgca tactgtgttt tgaacgaaac tgcagcaaag ttattcggca aaaacttcct     3300

tggtttctac tggaatagat taggtctttt ccaaaaggtt gattccgttg ctggtttagc     3360

taagttgatt ggttgtccag aagctaatgt tgttgctaca ttgaagcaat atgaggagtt     3420

atcttccaaa aagcttaatc cttgtccatt gactggcaag tctgtctttc cttgtgtttt     3480

aggcactcaa ggtccatact atgttgcctt ggttacccca tccattcact acactatggg     3540

tggttgtttg atttccccat ctgctgagat gcaaaccatt gacaactctg gtgttactcc     3600

tgtcagacgt ccaatcttag gcttattcgg tgctggtgaa gttactggcg gtgtccatgg     3660

tggtaacaga ttaggcggta actctttgtt agaatgtgtt gttttcggca agatcgctgg     3720

tgacagagct gcaaccatct tgcaaaagaa aaacaccggc ttatcaatga cagaatggtc     3780

tactgtcgtc ttaagagaag ttagagaagg tggtgtctat ggtgctggtt ccagagtttt     3840

gaggtttaac atgcctggtg cattacagag aactggttta gctttaggtc aattcatcgg     3900

tatcagaggt gattgggacg gtcacagatt gatcggttac tattctccaa tcactttacc     3960

tgatgatgtt ggtgttattg gtatcttagc tagagcagac aagggtagat tggcagaatg     4020

gatttctgca ttgcagccag gtgacgctgt tgagatgaag gcctgcggtg gtcttatcat     4080

tgacagaaga ttcgctgaaa gacatttctt tttccgtggt cataagatca gaaagttggc     4140

ccttatcggt ggtggtactg gtgttgcacc aatgttacaa atcgtcagag ctgctgtcaa     4200

aaagccattt gtcgattcaa ttgagtccat tcagttcatc tatgctgcag aggatgtttc     4260

cgagcttaca tacagaacct tacttgaatc ttacgaagag gaatatggtt cagaaaagtt     4320

taagtgtcac ttcgttttga ataacccacc agctcaatgg actgacggtg ttggtttcgt     4380

tgatactgca ttgttgagat ccgcagttca agcaccatca aatgatttgc ttgttgcaat     4440

ttgtggtcca ccaatcatgc aaagagcagt taagggtgca ttgaaaggtt taggttacaa     4500

tatgaatctt gttagaaccg ttgacgaaac tgaaccacca tcataattaa ttaacatctg     4560

aatgtaaaat gaacattaaa atgaattact aaactttacg tctactttac aatctataaa     4620

ctttgtttaa tcatataacg aaatacacta atacacaatc ctgtacgtat gtaatacttt     4680

tatccatcaa ggattgagaa aaaaaagtaa tgattccctg ggccattaaa acttagaccc     4740

ccaagcttgg ataggtcact ctctattttc gtttctccct tccctgatag aagggtgata     4800

tgtaattaag aataatatat aattttataa taaaagaatt catagcctca tgaaatcagc     4860

catttgcttt tgttcaacga tcttttgaaa ttgttgttgt tcttggtagt taagttgatc     4920

catcttggct tatgttgtgt gtatgttgta gttattctta gtatattcct gtcctgagtt     4980

tagtgaaaca taatatcgcc ttgaaatgaa aatgctgaaa ttcgtcgaca tacaattttt     5040

caaacttttt ttttttcttg gtgcacggac atgtttttaa aggaagtact ctataccagt     5100

tattcttcac aaatttaatt gctggagaat agatcttcaa cgctttaata aagtagtttg     5160

tttgtcaagg atggcgtcat acaaagaaag atcagaatca cacacttccc ctgttgctag     5220

gagacttttc tccatcatgg aggaaaagaa gtctaacctt tgtgcatcat tggatattac     5280

tgaaactgaa aagcttctct ctattttgga cactattggt ccttacatct gtctagttaa     5340

aacacacatc gatattgttt ctgattttac gtatgaagga actgtgttgc ctttgaagga     5400

gcttgccaag aaacataatt ttatgatttt tgaagataga aaatttgctg atattggtaa     5460

cactgttaaa aatcaatata aatctggtgt cttccgtatt gccgaatggg ctgacatcac     5520

taatgcacat ggtgtaacgg gtgcaggtat tgtttctggc ttgaaggagg cagcccaaga     5580

aacaaccagt gaacctagag gtttgctaat gcttgctgag ttatcatcaa agggttcttt     5640

agcatatggt gaatatacag aaaaaacagt agaaattgct aaatctgata aagagtttgt     5700

cattggtttt attgcgcaac acgatatggg cggtagagaa gaaggttttg actggatcat     5760

tatgactcca ggggttggtt tagatgacaa aggtgatgca cttggtcaac aatatagaac     5820

tgttgatgaa gttgtaaaga ctggaacgga tatcataatt gttggtagag gtttgtacgg     5880

tcaaggaaga gatcctatag agcaagctaa aagataccaa caagctggtt ggaatgctta     5940

tttaaacaga tttaaatgat tcttacacaa agatttgata catgtacact agtttaaata     6000

agcatgaaaa gaattacaca agcaaaaaaa aaaaaataaa tgaggtactt tacgttcacc     6060

tacaaccaaa aaaactagat agagtaaaat cttaagattt agaaaaagtt gtttaacaaa     6120

ggctttagta tgtgaatttt taatgtagca aagcgataac taataaacat aaacaaaagt     6180

atggttttct ttatcagtca aatcattatc gattgattgt tccgcgtatc tgcagatagc     6240

ctcatgaaat cagccatttg cttttgttca acgatctttt gaaattgttg ttgttcttgg     6300

tagttaagtt gatccatctt ggcttatgtt gtgtgtatgt tgtagttatt cttagtatat     6360

tcctgtcctg agtttagtga aacataatat cgccttgaaa tgaaaatgct gaaattcgtc     6420

gacatacaat ttttcaaact tttttttttt cttggtgcac ggacatgttt ttaaaggaag     6480

tactctatac cagttattct tcacaaattt aattgctgga gaatagatct tcaacgcccc     6540

gggggatctg gatccgcggc cgctgagctc gtctgatatt tgctaaattg aaatgaacct     6600

taccatgcca catctataga catcaaaacc attttcaatt tgtcgatatc ttttgcatat     6660

caaagtaata ccaagcatgt tcaaaaagaa aagaaagcat aactttaata ctctattcga     6720

aacattccga tccacaacac attagtcttt ttaggcccgt tgttcatctt tctattactt     6780

tattcctaac tgtattttta taattccggg tttataaaag attaaactaa tatagcgcat     6840

tctttttggg tacaaacata cataacggag gttt                                 6874


<210>  12
<211>  4127
<212>  DNA
<213>  Artificial

<220>
<223>  MDH gene integration fragment

<400>  12
gttaacccgt ttcgatggga ttcccagaag tggatactat actgtctgca atgcactaca       60

ctctaaaaaa gtattataca ttaccataca ttagcaaatc accaatactc tgcactgttt      120

cagtgtgtgc acattgctac ccaattggga aatcgcaggg aaaatgagac accccctcca      180

ttcgtattac gtaagacaat atcagggctg ccgaattcgg cagaaaagcc gagccggccg      240

agtcctcttg cacggagtgt gtccgaaaag ggcagctctg cagtggggga gaggaggtcg      300

cacgtctatg cggtgttggc atggcctgtg cgtgtacctg tcccctccct gggcatcccc      360

cactgcgcgc cttctccatt gggcgctgcg ggcactccgc gccgttaata caggaggggg      420

gggggaaagc ttaagattag agcgggtaca gtcagtgggt gtattgaccc catttctgtc      480

agtataaacc ccccgttgag ccgccggttt ggttgtttat ggataaaatt tttttttccc      540

cgcatggaga agattgaggg ggggaaggaa tgggaaaaag gccagagcca tctccacagc      600

ggaatccgac cgttaatggg gtgaaacacc cccaccaggt agagcaggaa gaatggggaa      660

acaaggtgga gagatggtca ttgttgggaa tagtgggaaa atgaggggga agagaatgac      720

tataaaatgg gaagggggtc caagttatcc aagcagtcca tttagagaag ggagcggccc      780

ctattggtag ttctttcccc ctctcaagct ggcgtgaaat gcaaccttac ggcgtctacg      840

ttactacaag gtccagaaag tgtaggtatt gctactattt ttatttttta ttggttctgg      900

agaaatgcag acagtcaatg aacacaactg tctcaatatg catctatgca catgcacaca      960

cacacacatc acaggtaccc ctacaaagag aggtctcttg ataatgtttc attaccacgt     1020

ggcatccccc cccccccccc caataaacaa gtggccgagt tcccctgttg cagaggagga     1080

caaaaaaacc gctggtgttg gtaccattat gcagcaacta gcacaacaaa caaccgaccc     1140

agacatacaa atcaacaaca cttcgccaaa gacacccttt ccagggagga tccactccca     1200

acgtctctcc ataatgtctc tgttggccca tgtctctgtc gttgacaccg taaccacacc     1260

aaccaacccg tccattgtac tgggatggtc gtccatagac acctctccaa cggggaacac     1320

ctcattcgta aaccgccaag gttaccgttc ctcctgactc gccccgttgt tgatgctgcg     1380

cacctgtggt tgcccaacat ggttgtatat cgtgtaacca caccaacaca tgtgcagcac     1440

atgtgtttaa aagagtgtca tggaggtgga tcatgatgga agtggacttt accacttggg     1500

aactgtctcc actcccggga agaaaagacc cggcgtatca cgcggttgcc tcaatggggc     1560

aatttggaag gagaaatata gggaaaatca cgtcgctctc ggacggggaa gagttccaga     1620

ctatgagggg ggggggtggt atataaagac aggagatgtc cacccccaga gagaggaaga     1680

agttggaact ttagaagaga gagataactt tccccagtgt ccatcaatac acaaccaaac     1740

acaaactcta tatttacaca tataaccccc tctctagaat ggttaaagtt acagtttgtg     1800

gtgctgctgg tggtattggt caaccccttt ctttactctt gaagcaatcc tctcacatta     1860

ctcacttatc tctttatgat atcgttaata ctcctggtgt tgctgctgat cttagtcata     1920

tcgataccaa atccaaggtc actggtcatg taggtgctgc tcaacttgaa gaagctatca     1980

aggattctga tgttgtcgtt attcccgctg gtgtcccaag aaagccaggt atgacgcgtg     2040

atgatctttt caagattaat gctggtattg tacgtgattt ggctacagct gctgcaaagt     2100

acgctccaaa ggccttcatg tgtatcattt ctaacccagt caactcgact gtcccaatcg     2160

ttactgaagt attcaaacag cacaatgttt atgaccccaa aagaatcttt ggtgtaacaa     2220

cacttgatat tgttcgtgca tccacctttg tatccgaatt gattggaggt gaacctaatt     2280

cacttcgtgt tcccgtcatt ggtggtcaca gcggcgtaac catcttacct ttactctcac     2340

aggtccccgg cattgaaaag ttaaaccaag aacaaattga gaaggtaact catcgtattc     2400

aatttggtgg cgatgaagtt gtcaaggcca aggatggtgc tggttctgcc actctttcca     2460

tggcttatgc tggtgctcgt tttgctacaa acatcattga ggctgctttt gctggaaaga     2520

agggcattgt tgaatgtacc tatgttcaat tggatgctga taaatctggt gcccaatctg     2580

tcaaggattt ggttggtagt gaacttgaat atttctctgt tcccgttgaa ttgggtccta     2640

gtggtgttga aaagatttta cccattggaa acgttaatga atatgaaaag aagttgttga     2700

acgaggcttc tcctgaatta aaaaccaaca ttgataaagg ttgtactttt gttactgaag     2760

gctcaaagtt gtaattaatt aatttatttt actagtttat ttttgctcct gagaatagga     2820

ttacaaacac ttaaagtctt taattacaac tatatataat attctgttgg ttttcttgaa     2880

ttggttcgct gcgattcatg cctcccattc accaaaggtg gagtgggaaa taacggtttt     2940

actgcggtaa ttagcagagg caagaacagg atacactttt tgatgataaa tctgtattat     3000

agtcgagcct atttaggaaa tcaaattttc ttgtgtttac ttttcaaata aataatgttc     3060

gaaaattttt actttactcc ttcatttaac tataccagac gttatatcat caacaccttc     3120

tgaccatata cagctcaaga tgtttaagag tctgttaaat tttttcaatc catttcatgg     3180

agtaccagga ggtgctacaa aaggaattca tagcctcatg aaatcagcca tttgcttttg     3240

ttcaacgatc ttttgaaatt gttgttgttc ttggtagtta agttgatcca tcttggctta     3300

tgttgtgtgt atgttgtagt tattcttagt atattcctgt cctgagttta gtgaaacata     3360

atatcgcctt gaaatgaaaa tgctgaaatt cgtcgacata caatttttca aacttttttt     3420

ttttcttggt gcacggacat gtttttaaag gaagtactct ataccagtta ttcttcacaa     3480

atttaattgc tggagaatag atcttcaacg ctttaataaa gtagtttgtt tgtcaaggat     3540

ggcgtcatac aaagaaagat cagaatcaca cacttcccct gttgctagga gacttttctc     3600

catcatggag gaaaagaagt ctaacctttg tgcatcattg gatattactg aaactgaaaa     3660

gcttctctct attttggaca ctattggtcc ttacatctgt ctagttaaaa cacacatcga     3720

tattgtttct gattttacgt atgaaggaac tgtgttgcct ttgaaggagc ttgccaagaa     3780

acataatttt atgatttttg aagatagaaa atttgctgat attggtaaca ctgttaaaaa     3840

tcaatataaa tctggtgtct tccgtattgc cgaatgggct gacatcacta atgcacatgg     3900

tgtaacgggt gcaggtattg tttctggctt gaaggaggca gcccaagaaa caaccagtga     3960

acctagaggt ttgctaatgc ttgctgagtt atcatcaaag ggttctttag catatggtga     4020

atatacagaa aaaacagtag aaattgctaa atctgataaa gagtttgtca ttggttttat     4080

tgcgcaacac gatatgggcg gtagagaaga aggttttgac tccgcgg                   4127


<210>  13
<211>  987
<212>  DNA
<213>  Rhizopus delemar

<400>  13
atggttaaag ttacagtttg tggtgctgct ggtggtattg gtcaacccct ttctttactc       60

ttgaagcaat cctctcacat tactcactta tctctttatg atatcgttaa tactcctggt      120

gttgctgctg atcttagtca tatcgatacc aaatccaagg tcactggtca tgtaggtgct      180

gctcaacttg aagaagctat caaggattct gatgttgtcg ttattcccgc tggtgtccca      240

agaaagccag gtatgacgcg tgatgatctt ttcaagatta atgctggtat tgtacgtgat      300

ttggctacag ctgctgcaaa gtacgctcca aaggccttca tgtgtatcat ttctaaccca      360

gtcaactcga ctgtcccaat cgttactgaa gtattcaaac agcacaatgt ttatgacccc      420

aaaagaatct ttggtgttac aacacttgat attgttcgtg catccacctt tgtatccgaa      480

ttgattggag gtgaacctaa ttcacttcgt gttcccgtca ttggtggtca cagcggcgta      540

accatcttac ctttactctc acaggtcccc ggcattgaaa agttaaacca agaacaaatt      600

gagaaggtaa ctcatcgtat tcaatttggt ggcgatgaag ttgtcaaggc caaggatggt      660

gctggttctg ccactctttc catggcttat gctggtgctc gttttgctac aaacatcatt      720

gaggctgctt ttgctggaaa gaagggcatt gttgaatgta cctatgttca attggatgct      780

gataaatctg gtgcccaatc tgtcaaggat ttggttggta gtgaacttga atatttctct      840

gttcccgttg aattgggtcc tagtggtgtt gaaaagattt tacccattgg aaacgttaat      900

gaatatgaaa agaagttgtt gaacgaggct tctcctgaat taaaaaccaa cattgataaa      960

ggttgtactt ttgttactga aggctaa                                          987


<210>  14
<211>  4112
<212>  DNA
<213>  Artificial

<220>
<223>  A. succinogenes FUM gene integration fragment

<400>  14
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttcctcg acatttgctg caacggcaac atcaatgtcc acgtttacac     1200

acctacattt atatctatat ttatatttat atttatttat ttatgctact tagcttctat     1260

agttagttaa tgcactcacg atattcaaaa ttgacaccct tcaactactc cctactattg     1320

tctactactg tctactactc ctctttacta tagctgctcc caataggctc caccaatagg     1380

ctctgtcaat acattttgcg ccgccacctt tcaggttgtg tcactcctga aggaccatat     1440

tgggtaatcg tgcaatttct ggaagagagt ccgcgagaag tgaggccccc actgtaaatc     1500

ctcgaggggg catggagtat ggggcatgga ggatggagga tggggggggg gggggggaaa     1560

ataggtagcg aaaggacccg ctatcacccc acccggagaa ctcgttgccg ggaagtcata     1620

tttcgacact ccggggagtc tataaaaggc gggttttgtc ttttgccagt tgatgttgct     1680

gagaggactt gtttgccgtt tcttccgatt taacagtata gaatcaacca ctgttaatta     1740

tacacgttat actaacacaa caaaaacaaa aacaacgaca acaacaacaa catctagaat     1800

gatcattatg actttccgta ttgagaagga tactatgggt gaagttcaag tcccagctga     1860

taagtattgg gctgcccaga ccgaaagatc tagaaacaac ttcaagattg gtccagctgc     1920

ttctatgcca catgaaatca ttgaagcttt tggttacttg aaaaaggcag ctgcatacgc     1980

taacgctgac ttgggtgttt tgccagctga aaagagagat ttgattgccc aagcttgtga     2040

cgaaatctta gccagaaagc ttgacgatca gttcccattg gttatctggc aaacaggttc     2100

tggtacccaa tccaatatga acttgaatga ggttatcgct aatagagcac atgttatcaa     2160

tggtggcaag ttgggtgaaa agtctatcat tcaccctaat gacgatgtca acaaatccca     2220

atcttctaat gacacttatc caacagcaat gcatattgcc acttacaaaa aggttgttga     2280

agctaccatc cctgcaattg aaagattaca aaagacctta gcagctaagt cagaagagtt     2340

taaggatgtt gtcaaaatcg gtaggactca tcttatggat gccaccccat taaccttggg     2400

tcaagagttc tctggttatg ctgcacaatt gtccttcggt ttagcagcaa tcaaaaacac     2460

cttgcctcat ttgagacaat tagcattagg tggtactgca gtcggtactg gtcttaacac     2520

tccaaaaggt tatgatgtta aagttgcaga atacattgcc aagtttactg gtttaccatt     2580

catcactgct gaaaacaagt tcgaggcctt agcaactcac gatgctattg tcgaaaccca     2640

cggtgcctta aagcaggttg caatgtcact tttcaagatc gcaaacgaca ttagattgtt     2700

ggcatcaggt ccaagatctg gcattggcga gatccttatc cctgaaaacg aaccaggttc     2760

atccattatg ccaggcaagg ttaaccctac tcaatgtgaa gcaatgacaa tggttgcagc     2820

acaagtctta ggtaatgata caacaatctc cttcgctggc tctcaaggtc acttcgaatt     2880

gaatgtcttt aagccagtta tggctgctaa ctttttgcaa tctgctcaac ttattgctga     2940

tgtttgcatt tcctttgacg aacactgtgc ttccggtatc aagcctaata ccccacgtat     3000

tcaacatttg ttagaatcct ccttaatgtt agtcaccgca ttgaacaccc acattggtta     3060

cgaaaatgca gctaagattg ctaagaccgc tcacaaaaac ggtactacat taagagaaga     3120

ggccattaac ttaggtttag tttctgctga agattttgat aagtgggtta gaccagaaga     3180

tatggttggt tccttgaagt aattaattaa catctgaatg taaaatgaac attaaaatga     3240

attactaaac tttacgtcta ctttacaatc tataaacttt gtttaatcat ataacgaaat     3300

acactaatac acaatcctgt acgtatgtaa tacttttatc catcaaggat tgagaaaaaa     3360

aagtaatgat tccctgggcc attaaaactt agacccccaa gcttggatag gtcactctct     3420

attttcgttt ctcccttccc tgatagaagg gtgatatgta attaagaata atatataatt     3480

ttataataaa agcggccgca ccaggggttt agtgaagtca ccaattaaga ttgttggttt     3540

gagtgagttg ccaaagatct atgaattgat ggagcaaggt aagattttag gcagatatgt     3600

tgttgacact tcgaaatgat gggctgactt gggtgtactg gtgtgacgtt tttatgtgta     3660

tattgatatg catgggggat gtatagtgat gaggagtaga gtatataacg aaatgaaatg     3720

aaataatatg atatgataag ataagatgag atcaatacga taatataaga tgcgacatga     3780

ggagttcaat gtagcatact acacgatgct gcagtacaac tctgatacgc tagactatac     3840

tatacaaaac tgtagtacac tatacgttag tgtagtatcc agaaacaaca ctgctttata     3900

gtacaataca actctataat actatagtat actatgccaa accacgtaat accataatat     3960

gctccacgac atggtacaat gtgctatact tcatactatt ataccatata tactccgata     4020

tattattgat atactatttt atactataat accataccac acaacactac attacaacga     4080

gcaaccttac cataaatgtc agttatggcc gc                                   4112


<210>  15
<211>  1404
<212>  DNA
<213>  Actinobacillus succinogenes

<400>  15
atgatcatta tgactttccg tattgagaag gatactatgg gtgaagttca agtcccagct       60

gataagtatt gggctgccca gaccgaaaga tctagaaaca acttcaagat tggtccagct      120

gcttctatgc cacatgaaat cattgaagct tttggttact tgaaaaaggc agctgcatac      180

gctaacgctg acttgggtgt tttgccagct gaaaagagag atttgattgc ccaagcttgt      240

gacgaaatct tagccagaaa gcttgacgat cagttcccat tggttatctg gcaaacaggt      300

tctggtaccc aatccaatat gaacttgaat gaggttatcg ctaatagagc acatgttatc      360

aatggtggca agttgggtga aaagtctatc attcacccta atgacgatgt caacaaatcc      420

caatcttcta atgacactta tccaacagca atgcatattg ccacttacaa aaaggttgtt      480

gaagctacca tccctgcaat tgaaagatta caaaagacct tagcagctaa gtcagaagag      540

tttaaggatg ttgtcaaaat cggtaggact catcttatgg atgccacccc attaaccttg      600

ggtcaagagt tctctggtta tgctgcacaa ttgtccttcg gtttagcagc aatcaaaaac      660

accttgcctc atttgagaca attagcatta ggtggtactg cagtcggtac tggtcttaac      720

actccaaaag gttatgatgt taaagttgca gaatacattg ccaagtttac tggtttacca      780

ttcatcactg ctgaaaacaa gttcgaggcc ttagcaactc acgatgctat tgtcgaaacc      840

cacggtgcct taaagcaggt tgcaatgtca cttttcaaga tcgcaaacga cattagattg      900

ttggcatcag gtccaagatc tggcattggc gagatcctta tccctgaaaa cgaaccaggt      960

tcatccatta tgccaggcaa ggttaaccct actcaatgtg aagcaatgac aatggttgca     1020

gcacaagtct taggtaatga tacaacaatc tccttcgctg gctctcaagg tcacttcgaa     1080

ttgaatgtct ttaagccagt tatggctgct aactttttgc aatctgctca acttattgct     1140

gatgtttgca tttcctttga cgaacactgt gcttccggta tcaagcctaa taccccacgt     1200

attcaacatt tgttagaatc ctccttaatg ttagtcaccg cattgaacac ccacattggt     1260

tacgaaaatg cagctaagat tgctaagacc gctcacaaaa acggtactac attaagagaa     1320

gaggccatta acttaggttt agtttctgct gaagattttg ataagtgggt tagaccagaa     1380

gatatggttg gttccttgaa gtaa                                            1404


<210>  16
<211>  3983
<212>  DNA
<213>  Artificial

<220>
<223>  R. delemar MDH gene integration fragment

<400>  16
gggccccata actgacattt atggtaaggt tgctcgttgt aatgtagtgt tgtgtggtat       60

ggtattatag tataaaatag tatatcaata atatatcgga gtatatatgg tataatagta      120

tgaagtatag cacattgtac catgtcgtgg agcatattat ggtattacgt ggtttggcat      180

agtatactat agtattatag agttgtattg tactataaag cagtgttgtt tctggatact      240

acactaacgt atagtgtact acagttttgt atagtatagt ctagcgtatc agagttgtac      300

tgcagcatcg tgtagtatgc tacattgaac tcctcatgtc gcatcttata ttatcgtatt      360

gatctcatct tatcttatca tatcatatta tttcatttca tttcgttata tactctactc      420

ctcatcacta tacatccccc atgcatatca atatacacat aaaaacgtca caccagtaca      480

cccaagtcag cccatcattt cgaagtgtca acaacatatc tgcctaaaat cttaccttgc      540

tccatcaatt catagatctt tggcaactca ctcaaaccaa caatcttaat tggtgacttc      600

actaaacccc tggtgcggcc gcggatccct cgagattggt agttctttcc ccctctcaag      660

ctggcgtgaa atgcaacctt acggcgtcta cgttactaca aggtccagaa agtgtaggta      720

ttgctactat ttttattttt tattggttct ggagaaatgc agacagtcaa tgaacacaac      780

tgtctcaata tgcatctatg cacatgcaca cacacacaca tcacaggtac ccctacaaag      840

agaggtctct tgataatgtt tcattaccac gtggcatccc cccccccccc cccaataaac      900

aagtggccga gttcccctgt tgcagaggag gacaaaaaaa ccgctggtgt tggtaccatt      960

atgcagcaac tagcacaaca aacaaccgac ccagacatac aaatcaacaa cacttcgcca     1020

aagacaccct ttccagggag gatccactcc caacgtctct ccataatgtc tctgttggcc     1080

catgtctctg tcgttgacac cgtaaccaca ccaaccaacc cgtccattgt actgggatgg     1140

tcgtccatag acacctctcc aacggggaac acctcattcg taaaccgcca aggttaccgt     1200

tcctcctgac tcgccccgtt gttgatgctg cgcacctgtg gttgcccaac atggttgtat     1260

atcgtgtaac cacaccaaca catgtgcagc acatgtgttt aaaagagtgt catggaggtg     1320

gatcatgatg gaagtggact ttaccacttg ggaactgtct ccactcccgg gaagaaaaga     1380

cccggcgtat cacgcggttg cctcaatggg gcaatttgga aggagaaata tagggaaaat     1440

cacgtcgctc tcggacgggg aagagttcca gactatgagg ggggggggtg gtatataaag     1500

acaggagatg tccaccccca gagagaggaa gaagttggaa ctttagaaga gagagataac     1560

tttccccagt gtccatcaat acacaaccaa acacaaactc tatatttaca catataaccc     1620

cctctctaga taaaatggtt aaagttacag tttgtggtgc tgctggtggt attggtcaac     1680

ccctttcttt actcttgaag caatcctctc acattactca cttatctctt tatgatatcg     1740

ttaatactcc tggtgttgct gctgatctta gtcatatcga taccaaatcc aaggtcactg     1800

gtcatgtagg tgctgctcaa cttgaagaag ctatcaagga ttctgatgtt gtcgttattc     1860

ccgctggtgt cccaagaaag ccaggtatga cgcgtgatga tcttttcaag attaatgctg     1920

gtattgtacg tgatttggct acagctgctg caaagtacgc tccaaaggcc ttcatgtgta     1980

tcatttctaa cccagtcaac tcgactgtcc caatcgttac tgaagtattc aaacagcaca     2040

atgtttatga ccccaaaaga atctttggtg taacaacact tgatattgtt cgtgcatcca     2100

cctttgtatc cgaattgatt ggaggtgaac ctaattcact tcgtgttccc gtcattggtg     2160

gtcacagcgg cgtaaccatc ttacctttac tctcacaggt ccccggcatt gaaaagttaa     2220

accaagaaca aattgagaag gtaactcatc gtattcaatt tggtggcgat gaagttgtca     2280

aggccaagga tggtgctggt tctgccactc tttccatggc ttatgctggt gctcgttttg     2340

ctacaaacat cattgaggct gcttttgctg gaaagaaggg cattgttgaa tgtacctatg     2400

ttcaattgga tgctgataaa tctggtgccc aatctgtcaa ggatttggtt ggtagtgaac     2460

ttgaatattt ctctgttccc gttgaattgg gtcctagtgg tgttgaaaag attttaccca     2520

ttggaaacgt taatgaatat gaaaagaagt tgttgaacga ggcttctcct gaattaaaaa     2580

ccaacattga taaaggttgt acttttgtta ctgaaggctc aaagttgtaa ttaattaatt     2640

tattttacta gtttattttt gctcctgaga ataggattac aaacacttaa agtctttaat     2700

tacaactata tataatattc tgttggtttt cttgaattgg ttcgctgcga ttcatgcctc     2760

ccattcacca aaggtggagt gggaaataac ggttttactg cggtaattag cagaggcaag     2820

aacaggatac actttttgat gataaatctg tattatagtc gagcctattt aggaaatcaa     2880

attttcttgt gtttactttt caaataaata atgttcgaaa atttttactt tactccttca     2940

tttaactata ccagacgtta tatcatcaac accttctgac catatacagc tcaagatgtt     3000

taagagtctg ttaaattttt tcaatccatt tcatggagta ccaggaggtg ctacaaaagg     3060

aattcatagc ctcatgaaat cagccatttg cttttgttca acgatctttt gaaattgttg     3120

ttgttcttgg tagttaagtt gatccatctt ggcttatgtt gtgtgtatgt tgtagttatt     3180

cttagtatat tcctgtcctg agtttagtga aacataatat cgccttgaaa tgaaaatgct     3240

gaaattcgtc gacatacaat ttttcaaact tttttttttt cttggtgcac ggacatgttt     3300

ttaaaggaag tactctatac cagttattct tcacaaattt aattgctgga gaatagatct     3360

tcaacgcttt aataaagtag tttgtttgtc aaggatggcg tcatacaaag aaagatcaga     3420

atcacacact tcccctgttg ctaggagact tttctccatc atggaggaaa agaagtctaa     3480

cctttgtgca tcattggata ttactgaaac tgaaaagctt ctctctattt tggacactat     3540

tggtccttac atctgtctag ttaaaacaca catcgatatt gtttctgatt ttacgtatga     3600

aggaactgtg ttgcctttga aggagcttgc caagaaacat aattttatga tttttgaaga     3660

tagaaaattt gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg     3720

tattgccgaa tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc     3780

tggcttgaag gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc     3840

tgagttatca tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat     3900

tgctaaatct gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag     3960

agaagaaggt tttgactccg cgg                                             3983


<210>  17
<211>  4060
<212>  DNA
<213>  Artificial

<220>
<223>  A. succinogenes FUM gene integration fragment

<400>  17
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttcctcg acatttgctg caacggcaac atcaatgtcc acgtttacac     1200

acctacattt atatctatat ttatatttat atttatttat ttatgctact tagcttctat     1260

agttagttaa tgcactcacg atattcaaaa ttgacaccct tcaactactc cctactattg     1320

tctactactg tctactactc ctctttacta tagctgctcc caataggctc caccaatagg     1380

ctctgtcaat acattttgcg ccgccacctt tcaggttgtg tcactcctga aggaccatat     1440

tgggtaatcg tgcaatttct ggaagagagt ccgcgagaag tgaggccccc actgtaaatc     1500

ctcgaggggg catggagtat ggggcatgga ggatggagga tggggggggg gggggggaaa     1560

ataggtagcg aaaggacccg ctatcacccc acccggagaa ctcgttgccg ggaagtcata     1620

tttcgacact ccggggagtc tataaaaggc gggttttgtc ttttgccagt tgatgttgct     1680

gagaggactt gtttgccgtt tcttccgatt taacagtata gaatcaacca ctgttaatta     1740

tacacgttat actaacacaa caaaaacaaa aacaacgaca acaacaacaa catctagata     1800

aaatgatcat tatgactttc cgtattgaga aggatactat gggtgaagtt caagtcccag     1860

ctgataagta ttgggctgcc cagaccgaaa gatctagaaa caacttcaag attggtccag     1920

ctgcttctat gccacatgaa atcattgaag cttttggtta cttgaaaaag gcagctgcat     1980

acgctaacgc tgacttgggt gttttgccag ctgaaaagag agatttgatt gcccaagctt     2040

gtgacgaaat cttagccaga aagcttgacg atcagttccc attggttatc tggcaaacag     2100

gttctggtac ccaatccaat atgaacttga atgaggttat cgctaataga gcacatgtta     2160

tcaatggtgg caagttgggt gaaaagtcta tcattcaccc taatgacgat gtcaacaaat     2220

cccaatcttc taatgacact tatccaacag caatgcatat tgccacttac aaaaaggttg     2280

ttgaagctac catccctgca attgaaagat tacaaaagac cttagcagct aagtcagaag     2340

agtttaagga tgttgtcaaa atcggtagga ctcatcttat ggatgccacc ccattaacct     2400

tgggtcaaga gttctctggt tatgctgcac aattgtcctt cggtttagca gcaatcaaaa     2460

acaccttgcc tcatttgaga caattagcat taggtggtac tgcagtcggt actggtctta     2520

acactccaaa aggttatgat gttaaagttg cagaatacat tgccaagttt actggtttac     2580

cattcatcac tgctgaaaac aagttcgagg ccttagcaac tcacgatgct attgtcgaaa     2640

cccacggtgc cttaaagcag gttgcaatgt cacttttcaa gatcgcaaac gacattagat     2700

tgttggcatc aggtccaaga tctggcattg gcgagatcct tatccctgaa aacgaaccag     2760

gttcatccat tatgccaggc aaggttaacc ctactcaatg tgaagcaatg acaatggttg     2820

cagcacaagt cttaggtaat gatacaacaa tctccttcgc tggctctcaa ggtcacttcg     2880

aattgaatgt ctttaagcca gttatggctg ctaacttttt gcaatctgct caacttattg     2940

ctgatgtttg catttccttt gacgaacact gtgcttccgg tatcaagcct aataccccac     3000

gtattcaaca tttgttagaa tcctccttaa tgttagtcac cgcattgaac acccacattg     3060

gttacgaaaa tgcagctaag attgctaaga ccgctcacaa aaacggtact acattaagag     3120

aagaggccat taacttaggt ttagtttctg ctgaagattt tgataagtgg gttagaccag     3180

aagatatggt tggttccttg aagtaattaa ttaacatctg aatgtaaaat gaacattaaa     3240

atgaattact aaactttacg tctactttac aatctataaa ctttgtttaa tcatataacg     3300

aaatacacta atacacaatc ctgtacgtat gtaatacttt tatccatcaa ggattgagaa     3360

aaaaaagtaa tgattccctg ggccattaaa acttagaccc ccaagcttgg ataggtcact     3420

ctctattttc gtttctccct tccctgatag aagggtgata tgtaattaag aataatatat     3480

aattttataa taaaagcggc cgcctccctt ctctaaatgg actgcttgga taacttggac     3540

ccccttccca ttttatagtc attctcttcc ccctcatttt cccactattc ccaacaatga     3600

ccatctctcc accttgtttc cccattcttc ctgctctacc tggtgggggt gtttcacccc     3660

attaacggtc ggattccgct gtggagatgg ctctggcctt tttcccattc cttccccccc     3720

tcaatcttct ccatgcgggg aaaaaaaaat tttatccata aacaaccaaa ccggcggctc     3780

aacggggggt ttatactgac agaaatgggg tcaatacacc cactgactgt acccgctcta     3840

atcttaagct ttcccccccc cctcctgtat taacggcgcg gagtgcccgc agcgcccaat     3900

ggagaaggcg cgcagtgggg gatgcccagg gaggggacag gtacacgcac aggccatgcc     3960

aacaccgcat agacgtgcga cctcctctcc cccactgcag agctgccctt ttcggacaca     4020

ctccgtgcaa gaggactcgg ccggctcggc ttttctgccg                           4060


<210>  18
<211>  3835
<212>  DNA
<213>  Artificial

<220>
<223>  5' integration fragment

<400>  18
aactactatg tacactgtat aagtaaaaag acgatacccc cctcccactc tgggtgctac       60

ggtgtagatc tctccgtaaa cacaaaaagg cggctcagat gataattggg gtccgggcgc      120

aaccggaagg ggggagagag gggagcgatg gcttctcctc cggggggcta cgggagtttc      180

ctctttggga aggataaaga ggggatggat tgatacaaga ttctgagaac ctattacgat      240

gatgttcagt ggtattttgt cttttgttat ttaaagggag gggactttcc tcaatacctt      300

agttgtaaaa ttacgctatt atctttaacc ctttcttttg agcaataatt aaaaagagcg      360

gccgcgagtc catcggttcc tgtcagatgg gatactcttg acgtggaaaa ttcaaacaga      420

aaaaaaaccc caataatgaa aaataacact acgttatatc cgtggtatcc tctatcgtat      480

cgtatcgtag cgtatcgtag cgtaccgtat cacagtatag tctaatattc cgtatcttat      540

tgtatcctat cctattcgat cctattgtat ttcagtgcac cattttaatt tctattgcta      600

taatgtcctt attagttgcc actgtgaggt gaccaatgga cgagggcgag ccgttcagaa      660

gccgcgaagg gtgttcttcc catgaatttc ttaaggaggg cggctcagct ccgagagtga      720

ggcgagacgt ctcggtcagc gtatccccct tcctcggctt ttacaaatga tgcgctctta      780

atagtgtgtc gttatccttt tggcattgac gggggaggga aattgattga gcgcatccat      840

atttttgcgg actgctgagg acaatggtgg tttttccggg tggcgtgggc tacaaatgat      900

acgatggttt ttttcttttc ggagaaggcg tataaaaagg acacggagaa cccatttatt      960

ctaaaaacag ttgagcttct ttaattattt tttgatataa tattctatta ttatatattt     1020

tcttcccaat aaaacaaaat aaaacaaaac acagcaaaac acaaaaagct agcggcgcgc     1080

cttctgtctt tgattttctt atgttattca aaacatctgc cccaaaatct aacgattata     1140

tatattccta cgtataactg tatagctaat tattgattta tttgtacata aaaaccacat     1200

aaatgtaaaa gcaagaaaaa aaataactaa ggagaaggat caatatctca tttataatgc     1260

tcgccaaagc agcgtacgtg aattttaatc aagacatcaa caaatcttgc aacttggtta     1320

tatcgcttct tcacccactc acccgctttt ctacattgtt gaacacaaat atatacaggg     1380

gtatgtctca aggtcaagtg cagtttcaac agagactacc tcaaggtacc tcttcagaaa     1440

tgcagaactt cactcttgat cagattttct ccgaattaaa ggaggcctat tggtagttct     1500

ttccccctct caagctggcg tgaaatgcaa ccttacggcg tctacgttac tacaaggtcc     1560

agaaagtgta ggtattgcta ctatttttat tttttattgg ttctggagaa atgcagacag     1620

tcaatgaaca caactgtctc aatatgcatc tatgcacatg cacacacaca cacatcacag     1680

gtacccctac aaagagaggt ctcttgataa tgtttcatta ccacgtggca tccccccccc     1740

cccccccaat aaacaagtgg ccgagttccc ctgttgcaga ggaggacaaa aaaaccgctg     1800

gtgttggtac cattatgcag caactagcac aacaaacaac cgacccagac atacaaatca     1860

acaacacttc gccaaagaca ccctttccag ggaggatcca ctcccaacgt ctctccataa     1920

tgtctctgtt ggcccatgtc tctgtcgttg acaccgtaac cacaccaacc aacccgtcca     1980

ttgtactggg atggtcgtcc atagacacct ctccaacggg gaacacctca ttcgtaaacc     2040

gccaaggtta ccgttcctcc tgactcgccc cgttgttgat gctgcgcacc tgtggttgcc     2100

caacatggtt gtatatcgtg taaccacacc aacacatgtg cagcacatgt gtttaaaaga     2160

gtgtcatgga ggtggatcat gatggaagtg gactttacca cttgggaact gtctccactc     2220

ccgggaagaa aagacccggc gtatcacgcg gttgcctcaa tggggcaatt tggaaggaga     2280

aatataggga aaatcacgtc gctctcggac ggggaagagt tccagactat gagggggggg     2340

ggtggtatat aaagacagga gatgtccacc cccagagaga ggaagaagtt ggaactttag     2400

aagagagaga taactttccc cagtgtccat caatacacaa ccaaacacaa actctatatt     2460

tacacatata accccctctc tagattaatt aatttatttt actagtttat ttttgctcct     2520

gagaatagga ttacaaacac ttaaagtctt taattacaac tatatataat attctgttgg     2580

ttttcttgaa ttggttcgct gcgattcatg cctcccattc accaaaggtg gagtgggaaa     2640

taacggtttt actgcggtaa ttagcagagg caagaacagg atacactttt tgatgataaa     2700

tctgtattat agtcgagcct atttaggaaa tcaaattttc ttgtgtttac ttttcaaata     2760

aataatgttc gaaaattttt actttactcc ttcatttaac tataccagac gttatatcat     2820

caacaccttc tgaccatata cagctcaaga tgtttaagag tctgttaaat tttttcaatc     2880

catttcatgg agtaccagga ggtgctacaa aaggaattca tagcctcatg aaatcagcca     2940

tttgcttttg ttcaacgatc ttttgaaatt gttgttgttc ttggtagtta agttgatcca     3000

tcttggctta tgttgtgtgt atgttgtagt tattcttagt atattcctgt cctgagttta     3060

gtgaaacata atatcgcctt gaaatgaaaa tgctgaaatt cgtcgacata caatttttca     3120

aacttttttt ttttcttggt gcacggacat gtttttaaag gaagtactct ataccagtta     3180

ttcttcacaa atttaattgc tggagaatag atcttcaacg ctttaataaa gtagtttgtt     3240

tgtcaaggat ggcgtcatac aaagaaagat cagaatcaca cacttcccct gttgctagga     3300

gacttttctc catcatggag gaaaagaagt ctaacctttg tgcatcattg gatattactg     3360

aaactgaaaa gcttctctct attttggaca ctattggtcc ttacatctgt ctagttaaaa     3420

cacacatcga tattgtttct gattttacgt atgaaggaac tgtgttgcct ttgaaggagc     3480

ttgccaagaa acataatttt atgatttttg aagatagaaa atttgctgat attggtaaca     3540

ctgttaaaaa tcaatataaa tctggtgtct tccgtattgc cgaatgggct gacatcacta     3600

atgcacatgg tgtaacgggt gcaggtattg tttctggctt gaaggaggca gcccaagaaa     3660

caaccagtga acctagaggt ttgctaatgc ttgctgagtt atcatcaaag ggttctttag     3720

catatggtga atatacagaa aaaacagtag aaattgctaa atctgataaa gagtttgtca     3780

ttggttttat tgcgcaacac gatatgggcg gtagagaaga aggttttgac tccgc          3835


<210>  19
<211>  3883
<212>  DNA
<213>  Artificial

<220>
<223>  Integration fragment targeted to MAE gene

<400>  19
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttaaaca gcaatttgag gaaggaatag gagaaggaga agcaatttct     1200

aggaaagagc aaggtgtgca acagcatgct ctgaatgata ttttcagcaa tagttcagtt     1260

gaagaacctg ttggcgtatc tacatcactt cctacaaaca acaccacgaa ttgcgtccgt     1320

ggtgacgcaa ctacgaatgg cattgtcaat gccaatgcca gtgcacatac acgtgcaagt     1380

cccaccggtt ccctgcccgg ctatggtaga gacaagaagg acgataccgg catcgacatc     1440

aacagtttca acagcaatgc gtttggcgtc gacgcgtcga tggggctgcc gtatttggat     1500

ttggacgggc tagatttcga tatggatatg gatatggata tggatatgga gatgaatttg     1560

aatttagatt tgggtcttga tttggggttg gaattaaaag gggataacaa tgagggtttt     1620

cctgttgatt taaacaatgg acgtgggagg tgattgattt aacctgatcc aaaaggggta     1680

tgtctatttt ttagagtgtg tctttgtgtc aaattatggt agaatgtgta aagtagtata     1740

aactttcctc tcaaatgacg aggtttaaaa caccccccgg gtgagccgag ccgagaatgg     1800

ggcaattgtt caatgtgaaa tagaagtatc gagtgagaaa cttgggtgtt ggccagccaa     1860

gggggaagga aaatggcgcg aatgctcagg tgagattgtt ttggaattgg gtgaagcgag     1920

gaaatgagcg acccggaggt tgtgacttta gtggcggagg aggaacggga ggaaaaggcc     1980

aagagggaaa gtgtatataa gggggagcaa tttgccaacc aggatagaat tggatgagtt     2040

ataattctac tgtatttatt gtataattta tttctccttt tatatcaaac acattacaaa     2100

acacacaaaa catacaaaca tacacagcta gcaaaggcgc gccatctaat agtttaatca     2160

cagcttatag tctactatag ttttcttttt taaacattgt tgtattttgt cccccccctc     2220

taattgatga tgattatcct ataagaatcc aataaaacga tggaaactaa taccctctcc     2280

tttgtcatgt ggtctttagt atttcttgaa cattggctct gatttctcga ctttatagtc     2340

ctattaaaat cgctgttagt tctcgatcgt tgtatctcgt ttcttgtctc tttggtggat     2400

gattttgcgt gcgaacatgt ttttttccct ttctctcacc atcatcgtgt agttcttgtc     2460

accatccccc ccaccccttc cttctctcat tgattctata agagcttatc cacagaggtg     2520

cagtaacgag gtagtttaac cttcgagtgg atcaaaatgt cacacaggcc tcgacatttg     2580

ctgcaacggc aacatcaatg tccacgttta cacacctaca tttatatcta tatttatatt     2640

tatatttatt tatttatgct acttagcttc tatagttagt taatgcactc acgatattca     2700

aaattgacac ccttcaacta ctccctacta ttgtctacta ctgtctacta ctcctcttta     2760

ctatagctgc tcccaatagg ctccaccaat aggctctgtc aatacatttt gcgccgccac     2820

ctttcaggtt gtgtcactcc tgaaggacca tattgggtaa tcgtgcaatt tctggaagag     2880

agtccgcgag aagtgaggcc cccactgtaa atcctcgagg gggcatggag tatggggcat     2940

ggaggatgga ggatgggggg gggggggggg aaaataggta gcgaaaggac ccgctatcac     3000

cccacccgga gaactcgttg ccgggaagtc atatttcgac actccgggga gtctataaaa     3060

ggcgggtttt gtcttttgcc agttgatgtt gctgagagga cttgtttgcc gtttcttccg     3120

atttaacagt atagaatcaa ccactgttaa ttatacacgt tatactaaca caacaaaaac     3180

aaaaacaacg acaacaacaa caacatctag ataattaatt aacatctgaa tgtaaaatga     3240

acattaaaat gaattactaa actttacgtc tactttacaa tctataaact ttgtttaatc     3300

atataacgaa atacactaat acacaatcct gtacgtatgt aatactttta tccatcaagg     3360

attgagaaaa aaaagtaatg attccctggg ccattaaaac ttagaccccc aagcttggat     3420

aggtcactct ctattttcgt ttctcccttc cctgatagaa gggtgatatg taattaagaa     3480

taatatataa ttttataata aaagcggccg cacacataca cattatcaaa tgcatttatt     3540

cctaatatca cactaaaacg tattatataa ttttaatctt tatagacttc atagcaccaa     3600

ttggatttgc tttctttcag aataccgcac ttaatctcaa tgtacgtaac gtaggcaaaa     3660

tctgtcgata aggatctgta tgccgtaaac ggaaactcca agcgcccaga aaacttacat     3720

tatattcttg ccagtttcat ctcaccagcc agtcacagtt taaaaggttt gattgcgttt     3780

cttgtttcgt cggattcagt gctaattggt aacgcactgt accgccacac caaagcaaaa     3840

atgcagaaac aaacaacaat gagtgtatgt ttaccaactt tgg                       3883


<210>  20
<211>  5722
<212>  DNA
<213>  Artificial

<220>
<223>  E. coli SthA gene integration fragment

<400>  20
aactactatg tacactgtat aagtaaaaag acgatacccc cctcccactc tgggtgctac       60

ggtgtagatc tctccgtaaa cacaaaaagg cggctcagat gataattggg gtccgggcgc      120

aaccggaagg ggggagagag gggagcgatg gcttctcctc cggggggcta cgggagtttc      180

ctctttggga aggataaaga ggggatggat tgatacaaga ttctgagaac ctattacgat      240

gatgttcagt ggtattttgt cttttgttat ttaaagggag gggactttcc tcaatacctt      300

agttgtaaaa ttacgctatt atctttaacc ctttcttttg agcaataatt aaaaagagcg      360

gccgcgagtc catcggttcc tgtcagatgg gatactcttg acgtggaaaa ttcaaacaga      420

aaaaaaaccc caataatgaa aaataacact acgttatatc cgtggtatcc tctatcgtat      480

cgtatcgtag cgtatcgtag cgtaccgtat cacagtatag tctaatattc cgtatcttat      540

tgtatcctat cctattcgat cctattgtat ttcagtgcac cattttaatt tctattgcta      600

taatgtcctt attagttgcc actgtgaggt gaccaatgga cgagggcgag ccgttcagaa      660

gccgcgaagg gtgttcttcc catgaatttc ttaaggaggg cggctcagct ccgagagtga      720

ggcgagacgt ctcggtcagc gtatccccct tcctcggctt ttacaaatga tgcgctctta      780

atagtgtgtc gttatccttt tggcattgac gggggaggga aattgattga gcgcatccat      840

atttttgcgg actgctgagg acaatggtgg tttttccggg tggcgtgggc tacaaatgat      900

acgatggttt ttttcttttc ggagaaggcg tataaaaagg acacggagaa cccatttatt      960

ctaaaaacag ttgagcttct ttaattattt tttgatataa tattctatta ttatatattt     1020

tcttcccaat aaaacaaaat aaaacaaaac acagcaaaac acaaaaagct agcctgaaag     1080

ggaaccataa tgggtaagat cgcaccacat tagcgggctc gaagatggat cttgcgaatg     1140

ggtgacacca gtcataaggc ctcgttgtcc cagcatacct cccgcgctat ctaattgctt     1200

cgctctccat tgttcttggt aaacatcact ctggcttgat ggtgtcatct atgcccgcca     1260

agcctatcgg tctatggccc ggagtttgct ccgtcttcca attgcaatcg cacggaatcc     1320

gggatagaaa gaacgatacg cattcatacg attctcacgt tattggttgg tgaatcaaat     1380

gcacaacgaa cccaatcgcc ctggactcag cgtctaggcc ccccgtatgg ccgacgggga     1440

ctcagagcgt caatccacgt tgaagtcgag gttttggcag ttacagccct tgcaataagg     1500

tttttcggac agtctacttt gtcggcgcgc cttctgtctt tgattttctt atgttattca     1560

aaacatctgc cccaaaatct aacgattata tatattccta cgtataactg tatagctaat     1620

tattgattta tttgtacata aaaaccacat aaatgtaaaa gcaagaaaaa aaataactaa     1680

ggagaaggat caatatctca tttataatgc tcgccaaagc agcgtacgtg aattttaatc     1740

aagacatcaa caaatcttgc aacttggtta tatcgcttct tcacccactc acccgctttt     1800

ctacattgtt gaacacaaat atatacaggg gtatgtctca aggtcaagtg cagtttcaac     1860

agagactacc tcaaggtacc tcttcagaaa tgcagaactt cactcttgat cagattttct     1920

ccgaattaaa ggaggcctat tggtagttct ttccccctct caagctggcg tgaaatgcaa     1980

ccttacggcg tctacgttac tacaaggtcc agaaagtgta ggtattgcta ctatttttat     2040

tttttattgg ttctggagaa atgcagacag tcaatgaaca caactgtctc aatatgcatc     2100

tatgcacatg cacacacaca cacatcacag gtacccctac aaagagaggt ctcttgataa     2160

tgtttcatta ccacgtggca tccccccccc cccccccaat aaacaagtgg ccgagttccc     2220

ctgttgcaga ggaggacaaa aaaaccgctg gtgttggtac cattatgcag caactagcac     2280

aacaaacaac cgacccagac atacaaatca acaacacttc gccaaagaca ccctttccag     2340

ggaggatcca ctcccaacgt ctctccataa tgtctctgtt ggcccatgtc tctgtcgttg     2400

acaccgtaac cacaccaacc aacccgtcca ttgtactggg atggtcgtcc atagacacct     2460

ctccaacggg gaacacctca ttcgtaaacc gccaaggtta ccgttcctcc tgactcgccc     2520

cgttgttgat gctgcgcacc tgtggttgcc caacatggtt gtatatcgtg taaccacacc     2580

aacacatgtg cagcacatgt gtttaaaaga gtgtcatgga ggtggatcat gatggaagtg     2640

gactttacca cttgggaact gtctccactc ccgggaagaa aagacccggc gtatcacgcg     2700

gttgcctcaa tggggcaatt tggaaggaga aatataggga aaatcacgtc gctctcggac     2760

ggggaagagt tccagactat gagggggggg ggtggtatat aaagacagga gatgtccacc     2820

cccagagaga ggaagaagtt ggaactttag aagagagaga taactttccc cagtgtccat     2880

caatacacaa ccaaacacaa actctatatt tacacatata accccctctc tagaatgcca     2940

cattcctacg attacgatgc catagtaata ggttccggcc ccggcggcga aggcgctgca     3000

atgggcctgg ttaagcaagg tgcgcgcgtc gcagttatcg agcgttatca aaatgttggc     3060

ggcggttgca cccactgggg caccatcccg tcgaaagctc tccgtcacgc cgtcagccgc     3120

attatagaat tcaatcaaaa cccactttac agcgaccatt cccgactgct ccgctcttct     3180

tttgccgata tccttaacca tgccgataac gtgattaatc aacaaacgcg catgcgtcag     3240

ggattttacg aacgtaatca ctgtgaaata ttgcagggaa acgctcgctt tgttgacgag     3300

catacgttgg cgctggattg cccggacggc agcgttgaaa cactaaccgc tgaaaaattt     3360

gttattgcct gcggctctcg tccatatcat ccaacagatg ttgatttcac ccatccacgc     3420

atttacgaca gcgactcaat tctcagcatg caccacgaac cgcgccatgt acttatctat     3480

ggtgctggag tgatcggctg tgaatatgcg tcgatcttcc gcggtatgga tgtaaaagtg     3540

gatctgatca acacccgcga tcgcctgctg gcatttctcg atcaagagat gtcagattct     3600

ctctcctatc acttctggaa cagtggcgta gtgattcgtc acaacgaaga gtacgagaag     3660

atcgaaggct gtgacgatgg tgtgatcatg catctgaagt cgggtaaaaa actgaaagct     3720

gactgcctgc tctatgccaa cggtcgcacc ggtaataccg attcgctggc gttacagaac     3780

attgggctag aaactgacag ccgcggacag ctgaaggtca acagcatgta tcagaccgca     3840

cagccacacg tttacgcggt gggcgacgtg attggttatc cgagcctggc gtcggcggcc     3900

tatgaccagg ggcgcattgc cgcgcaggcg ctggtaaaag gcgaagccac cgcacatctg     3960

attgaagata tccctaccgg tatttacacc atcccggaaa tcagctctgt gggcaaaacc     4020

gaacagcagc tgaccgcaat gaaagtgcca tatgaagtgg gccgcgccca gtttaaacat     4080

ctggcacgcg cacaaatcgt cggcatgaac gtgggcacgc tgaaaatttt gttccatcgg     4140

gaaacaaaag agattctggg tattcactgc tttggcgagc gcgctgccga aattattcat     4200

atcggtcagg cgattatgga acagaaaggt ggcggcaaca ctattgagta cttcgtcaac     4260

accaccttta actacccgac gatggcggaa gcctatcggg tagctgcgtt aaacggttta     4320

aaccgcctgt tttaaaactt tatcgaaatg gccatccatt cttgcgcgga tttaattaat     4380

ttattttact agtttatttt tgctcctgag aataggatta caaacactta aagtctttaa     4440

ttacaactat atataatatt ctgttggttt tcttgaattg gttcgctgcg attcatgcct     4500

cccattcacc aaaggtggag tgggaaataa cggttttact gcggtaatta gcagaggcaa     4560

gaacaggata cactttttga tgataaatct gtattatagt cgagcctatt taggaaatca     4620

aattttcttg tgtttacttt tcaaataaat aatgttcgaa aatttttact ttactccttc     4680

atttaactat accagacgtt atatcatcaa caccttctga ccatatacag ctcaagatgt     4740

ttaagagtct gttaaatttt ttcaatccat ttcatggagt accaggaggt gctacaaaag     4800

gaattcatag cctcatgaaa tcagccattt gcttttgttc aacgatcttt tgaaattgtt     4860

gttgttcttg gtagttaagt tgatccatct tggcttatgt tgtgtgtatg ttgtagttat     4920

tcttagtata ttcctgtcct gagtttagtg aaacataata tcgccttgaa atgaaaatgc     4980

tgaaattcgt cgacatacaa tttttcaaac tttttttttt tcttggtgca cggacatgtt     5040

tttaaaggaa gtactctata ccagttattc ttcacaaatt taattgctgg agaatagatc     5100

ttcaacgctt taataaagta gtttgtttgt caaggatggc gtcatacaaa gaaagatcag     5160

aatcacacac ttcccctgtt gctaggagac ttttctccat catggaggaa aagaagtcta     5220

acctttgtgc atcattggat attactgaaa ctgaaaagct tctctctatt ttggacacta     5280

ttggtcctta catctgtcta gttaaaacac acatcgatat tgtttctgat tttacgtatg     5340

aaggaactgt gttgcctttg aaggagcttg ccaagaaaca taattttatg atttttgaag     5400

atagaaaatt tgctgatatt ggtaacactg ttaaaaatca atataaatct ggtgtcttcc     5460

gtattgccga atgggctgac atcactaatg cacatggtgt aacgggtgca ggtattgttt     5520

ctggcttgaa ggaggcagcc caagaaacaa ccagtgaacc tagaggtttg ctaatgcttg     5580

ctgagttatc atcaaagggt tctttagcat atggtgaata tacagaaaaa acagtagaaa     5640

ttgctaaatc tgataaagag tttgtcattg gttttattgc gcaacacgat atgggcggta     5700

gagaagaagg ttttgactcc gc                                              5722


<210>  21
<211>  1401
<212>  DNA
<213>  Escherichia coli

<400>  21
atgccacatt cctacgatta cgatgccata gtaataggtt ccggccccgg cggcgaaggc       60

gctgcaatgg gcctggttaa gcaaggtgcg cgcgtcgcag ttatcgagcg ttatcaaaat      120

gttggcggcg gttgcaccca ctggggcacc atcccgtcga aagctctccg tcacgccgtc      180

agccgcatta tagaattcaa tcaaaaccca ctttacagcg accattcccg actgctccgc      240

tcttcttttg ccgatatcct taaccatgcc gataacgtga ttaatcaaca aacgcgcatg      300

cgtcagggat tttacgaacg taatcactgt gaaatattgc agggaaacgc tcgctttgtt      360

gacgagcata cgttggcgct ggattgcccg gacggcagcg ttgaaacact aaccgctgaa      420

aaatttgtta ttgcctgcgg ctctcgtcca tatcatccaa cagatgttga tttcacccat      480

ccacgcattt acgacagcga ctcaattctc agcatgcacc acgaaccgcg ccatgtactt      540

atctatggtg ctggagtgat cggctgtgaa tatgcgtcga tcttccgcgg tatggatgta      600

aaagtggatc tgatcaacac ccgcgatcgc ctgctggcat ttctcgatca agagatgtca      660

gattctctct cctatcactt ctggaacagt ggcgtagtga ttcgtcacaa cgaagagtac      720

gagaagatcg aaggctgtga cgatggtgtg atcatgcatc tgaagtcggg taaaaaactg      780

aaagctgact gcctgctcta tgccaacggt cgcaccggta ataccgattc gctggcgtta      840

cagaacattg ggctagaaac tgacagccgc ggacagctga aggtcaacag catgtatcag      900

accgcacagc cacacgttta cgcggtgggc gacgtgattg gttatccgag cctggcgtcg      960

gcggcctatg accaggggcg cattgccgcg caggcgctgg taaaaggcga agccaccgca     1020

catctgattg aagatatccc taccggtatt tacaccatcc cggaaatcag ctctgtgggc     1080

aaaaccgaac agcagctgac cgcaatgaaa gtgccatatg aagtgggccg cgcccagttt     1140

aaacatctgg cacgcgcaca aatcgtcggc atgaacgtgg gcacgctgaa aattttgttc     1200

catcgggaaa caaaagagat tctgggtatt cactgctttg gcgagcgcgc tgccgaaatt     1260

attcatatcg gtcaggcgat tatggaacag aaaggtggcg gcaacactat tgagtacttc     1320

gtcaacacca cctttaacta cccgacgatg gcggaagcct atcgggtagc tgcgttaaac     1380

ggtttaaacc gcctgtttta a                                               1401


<210>  22
<211>  5335
<212>  DNA
<213>  Artificial

<220>
<223>  RH E. coli integration fragment

<400>  22
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttaaaca gcaatttgag gaaggaatag gagaaggaga agcaatttct     1200

aggaaagagc aaggtgtgca acagcatgct ctgaatgata ttttcagcaa tagttcagtt     1260

gaagaacctg ttggcgtatc tacatcactt cctacaaaca acaccacgaa ttgcgtccgt     1320

ggtgacgcaa ctacgaatgg cattgtcaat gccaatgcca gtgcacatac acgtgcaagt     1380

cccaccggtt ccctgcccgg ctatggtaga gacaagaagg acgataccgg catcgacatc     1440

aacagtttca acagcaatgc gtttggcgtc gacgcgtcga tggggctgcc gtatttggat     1500

ttggacgggc tagatttcga tatggatatg gatatggata tggatatgga gatgaatttg     1560

aatttagatt tgggtcttga tttggggttg gaattaaaag gggataacaa tgagggtttt     1620

cctgttgatt taaacaatgg acgtgggagg tgattgattt aacctgatcc aaaaggggta     1680

tgtctatttt ttagagtgtg tctttgtgtc aaattatggt agaatgtgta aagtagtata     1740

aactttcctc tcaaatgacg aggtttaaaa caccccccgg gtgagccgag ccgagaatgg     1800

ggcaattgtt caatgtgaaa tagaagtatc gagtgagaaa cttgggtgtt ggccagccaa     1860

gggggaagga aaatggcgcg aatgctcagg tgagattgtt ttggaattgg gtgaagcgag     1920

gaaatgagcg acccggaggt tgtgacttta gtggcggagg aggaacggga ggaaaaggcc     1980

aagagggaaa gtgtatataa gggggagcaa tttgccaacc aggatagaat tggatgagtt     2040

ataattctac tgtatttatt gtataattta tttctccttt tatatcaaac acattacaaa     2100

acacacaaaa catacaaaca tacacagcta gcaaaggcgc gccatctaat agtttaatca     2160

cagcttatag tctactatag ttttcttttt taaacattgt tgtattttgt cccccccctc     2220

taattgatga tgattatcct ataagaatcc aataaaacga tggaaactaa taccctctcc     2280

tttgtcatgt ggtctttagt atttcttgaa cattggctct gatttctcga ctttatagtc     2340

ctattaaaat cgctgttagt tctcgatcgt tgtatctcgt ttcttgtctc tttggtggat     2400

gattttgcgt gcgaacatgt ttttttccct ttctctcacc atcatcgtgt agttcttgtc     2460

accatccccc ccaccccttc cttctctcat tgattctata agagcttatc cacagaggtg     2520

cagtaacgag gtagtttaac cttcgagtgg atcaaaatgt cacacaggcc tcgacatttg     2580

ctgcaacggc aacatcaatg tccacgttta cacacctaca tttatatcta tatttatatt     2640

tatatttatt tatttatgct acttagcttc tatagttagt taatgcactc acgatattca     2700

aaattgacac ccttcaacta ctccctacta ttgtctacta ctgtctacta ctcctcttta     2760

ctatagctgc tcccaatagg ctccaccaat aggctctgtc aatacatttt gcgccgccac     2820

ctttcaggtt gtgtcactcc tgaaggacca tattgggtaa tcgtgcaatt tctggaagag     2880

agtccgcgag aagtgaggcc cccactgtaa atcctcgagg gggcatggag tatggggcat     2940

ggaggatgga ggatgggggg gggggggggg aaaataggta gcgaaaggac ccgctatcac     3000

cccacccgga gaactcgttg ccgggaagtc atatttcgac actccgggga gtctataaaa     3060

ggcgggtttt gtcttttgcc agttgatgtt gctgagagga cttgtttgcc gtttcttccg     3120

atttaacagt atagaatcaa ccactgttaa ttatacacgt tatactaaca caacaaaaac     3180

aaaaacaacg acaacaacaa caacatctag aatgccacat tcctacgatt acgatgccat     3240

agtaataggt tccggccccg gcggcgaagg cgctgcaatg ggcctggtta agcaaggtgc     3300

gcgcgtcgca gttatcgagc gttatcaaaa tgttggcggc ggttgcaccc actggggcac     3360

catcccgtcg aaagctctcc gtcacgccgt cagccgcatt atagaattca atcaaaaccc     3420

actttacagc gaccattccc gactgctccg ctcttctttt gccgatatcc ttaaccatgc     3480

cgataacgtg attaatcaac aaacgcgcat gcgtcaggga ttttacgaac gtaatcactg     3540

tgaaatattg cagggaaacg ctcgctttgt tgacgagcat acgttggcgc tggattgccc     3600

ggacggcagc gttgaaacac taaccgctga aaaatttgtt attgcctgcg gctctcgtcc     3660

atatcatcca acagatgttg atttcaccca tccacgcatt tacgacagcg actcaattct     3720

cagcatgcac cacgaaccgc gccatgtact tatctatggt gctggagtga tcggctgtga     3780

atatgcgtcg atcttccgcg gtatggatgt aaaagtggat ctgatcaaca cccgcgatcg     3840

cctgctggca tttctcgatc aagagatgtc agattctctc tcctatcact tctggaacag     3900

tggcgtagtg attcgtcaca acgaagagta cgagaagatc gaaggctgtg acgatggtgt     3960

gatcatgcat ctgaagtcgg gtaaaaaact gaaagctgac tgcctgctct atgccaacgg     4020

tcgcaccggt aataccgatt cgctggcgtt acagaacatt gggctagaaa ctgacagccg     4080

cggacagctg aaggtcaaca gcatgtatca gaccgcacag ccacacgttt acgcggtggg     4140

cgacgtgatt ggttatccga gcctggcgtc ggcggcctat gaccaggggc gcattgccgc     4200

gcaggcgctg gtaaaaggcg aagccaccgc acatctgatt gaagatatcc ctaccggtat     4260

ttacaccatc ccggaaatca gctctgtggg caaaaccgaa cagcagctga ccgcaatgaa     4320

agtgccatat gaagtgggcc gcgcccagtt taaacatctg gcacgcgcac aaatcgtcgg     4380

catgaacgtg ggcacgctga aaattttgtt ccatcgggaa acaaaagaga ttctgggtat     4440

tcactgcttt ggcgagcgcg ctgccgaaat tattcatatc ggtcaggcga ttatggaaca     4500

gaaaggtggc ggcaacacta ttgagtactt cgtcaacacc acctttaact acccgacgat     4560

ggcggaagcc tatcgggtag ctgcgttaaa cggtttaaac cgcctgtttt aaaactttat     4620

cgaaatggcc atccattctt gcgcggattt aattaacatc tgaatgtaaa atgaacatta     4680

aaatgaatta ctaaacttta cgtctacttt acaatctata aactttgttt aatcatataa     4740

cgaaatacac taatacacaa tcctgtacgt atgtaatact tttatccatc aaggattgag     4800

aaaaaaaagt aatgattccc tgggccatta aaacttagac ccccaagctt ggataggtca     4860

ctctctattt tcgtttctcc cttccctgat agaagggtga tatgtaatta agaataatat     4920

ataattttat aataaaagcg gccgcacaca tacacattat caaatgcatt tattcctaat     4980

atcacactaa aacgtattat ataattttaa tctttataga cttcatagca ccaattggat     5040

ttgctttctt tcagaatacc gcacttaatc tcaatgtacg taacgtaggc aaaatctgtc     5100

gataaggatc tgtatgccgt aaacggaaac tccaagcgcc cagaaaactt acattatatt     5160

cttgccagtt tcatctcacc agccagtcac agtttaaaag gtttgattgc gtttcttgtt     5220

tcgtcggatt cagtgctaat tggtaacgca ctgtaccgcc acaccaaagc aaaaatgcag     5280

aaacaaacaa caatgagtgt atgtttacca actttggttt tgaaagttaa cccgc          5335


<210>  23
<211>  5642
<212>  DNA
<213>  Artificial

<220>
<223>  Codon-optimized E. coli Stha gene integration fragment

<400>  23
aaccccactc tgggtgctac ggtgtagatc tctccgtaaa cacaaaaagg cggctcagat       60

gataattggg gtccgggcgc aaccggaagg ggggagagag gggagcgatg gcttctcctc      120

cggggggcta cgggagtttc ctctttggga aggataaaga ggggatggat tgatacaaga      180

ttctgagaac ctattacgat gatgttcagt ggtattttgt cttttgttat ttaaagggag      240

gggactttcc tcaatacctt agttgtaaaa ttacgctatt atctttaacc ctttcttttg      300

agcaataatt aaaaagagcg gccgcgagtc catcggttcc tgtcagatgg gatactcttg      360

acgtggaaaa ttcaaacaga aaaaaaaccc caataatgaa aaataacact acgttatatc      420

cgtggtatcc tctatcgtat cgtatcgtag cgtatcgtag cgtaccgtat cacagtatag      480

tctaatattc cgtatcttat tgtatcctat cctattcgat cctattgtat ttcagtgcac      540

cattttaatt tctattgcta taatgtcctt attagttgcc actgtgaggt gaccaatgga      600

cgagggcgag ccgttcagaa gccgcgaagg gtgttcttcc catgaatttc ttaaggaggg      660

cggctcagct ccgagagtga ggcgagacgt ctcggtcagc gtatccccct tcctcggctt      720

ttacaaatga tgcgctctta atagtgtgtc gttatccttt tggcattgac gggggaggga      780

aattgattga gcgcatccat atttttgcgg actgctgagg acaatggtgg tttttccggg      840

tggcgtgggc tacaaatgat acgatggttt ttttcttttc ggagaaggcg tataaaaagg      900

acacggagaa cccatttatt ctaaaaacag ttgagcttct ttaattattt tttgatataa      960

tattctatta ttatatattt tcttcccaat aaaacaaaat aaaacaaaac acagcaaaac     1020

acaaaaagct agcctgaaag ggaaccataa tgggtaagat cgcaccacat tagcgggctc     1080

gaagatggat cttgcgaatg ggtgacacca gtcataaggc ctcgttgtcc cagcatacct     1140

cccgcgctat ctaattgctt cgctctccat tgttcttggt aaacatcact ctggcttgat     1200

ggtgtcatct atgcccgcca agcctatcgg tctatggccc ggagtttgct ccgtcttcca     1260

attgcaatcg cacggaatcc gggatagaaa gaacgatacg cattcatacg attctcacgt     1320

tattggttgg tgaatcaaat gcacaacgaa cccaatcgcc ctggactcag cgtctaggcc     1380

ccccgtatgg ccgacgggga ctcagagcgt caatccacgt tgaagtcgag gttttggcag     1440

ttacagccct tgcaataagg tttttcggac agtctacttt gtcggcgcgc cttctgtctt     1500

tgattttctt atgttattca aaacatctgc cccaaaatct aacgattata tatattccta     1560

cgtataactg tatagctaat tattgattta tttgtacata aaaaccacat aaatgtaaaa     1620

gcaagaaaaa aaataactaa ggagaaggat caatatctca tttataatgc tcgccaaagc     1680

agcgtacgtg aattttaatc aagacatcaa caaatcttgc aacttggtta tatcgcttct     1740

tcacccactc acccgctttt ctacattgtt gaacacaaat atatacaggg gtatgtctca     1800

aggtcaagtg cagtttcaac agagactacc tcaaggtacc tcttcagaaa tgcagaactt     1860

cactcttgat cagattttct ccgaattaaa ggaggcctat tggtagttct ttccccctct     1920

caagctggcg tgaaatgcaa ccttacggcg tctacgttac tacaaggtcc agaaagtgta     1980

ggtattgcta ctatttttat tttttattgg ttctggagaa atgcagacag tcaatgaaca     2040

caactgtctc aatatgcatc tatgcacatg cacacacaca cacatcacag gtacccctac     2100

aaagagaggt ctcttgataa tgtttcatta ccacgtggca tccccccccc cccccccaat     2160

aaacaagtgg ccgagttccc ctgttgcaga ggaggacaaa aaaaccgctg gtgttggtac     2220

cattatgcag caactagcac aacaaacaac cgacccagac atacaaatca acaacacttc     2280

gccaaagaca ccctttccag ggaggatcca ctcccaacgt ctctccataa tgtctctgtt     2340

ggcccatgtc tctgtcgttg acaccgtaac cacaccaacc aacccgtcca ttgtactggg     2400

atggtcgtcc atagacacct ctccaacggg gaacacctca ttcgtaaacc gccaaggtta     2460

ccgttcctcc tgactcgccc cgttgttgat gctgcgcacc tgtggttgcc caacatggtt     2520

gtatatcgtg taaccacacc aacacatgtg cagcacatgt gtttaaaaga gtgtcatgga     2580

ggtggatcat gatggaagtg gactttacca cttgggaact gtctccactc ccgggaagaa     2640

aagacccggc gtatcacgcg gttgcctcaa tggggcaatt tggaaggaga aatataggga     2700

aaatcacgtc gctctcggac ggggaagagt tccagactat gagggggggg ggtggtatat     2760

aaagacagga gatgtccacc cccagagaga ggaagaagtt ggaactttag aagagagaga     2820

taactttccc cagtgtccat caatacacaa ccaaacacaa actctatatt tacacatata     2880

accccctctc tagaatgcca cattcctatg actacgatgc cattgtcatt ggttccggtc     2940

caggtggtga aggtgctgca atgggcttag ttaagcaggg tgctagagtt gctgtcatcg     3000

aaagatatca aaatgttggt ggtggttgta ctcactgggg tacaattcca tctaaggcat     3060

tgagacatgc agtttccaga attattgagt ttaaccaaaa ccctttatac tctgatcatt     3120

caagattgtt gagatcatct tttgctgata ttttgaacca tgctgacaac gtcatcaacc     3180

aacaaactcg tatgcgtcaa ggcttctatg agagaaatca ttgtgagatt ttacaaggta     3240

acgctagatt tgtcgatgag catactcttg cattagactg tccagacggt tccgttgaga     3300

ctcttaccgc tgaaaaattc gttattgctt gtggttccag accataccac ccaaccgatg     3360

tcgatttcac tcaccctcgt atctacgatt ccgattctat tttgtctatg catcatgaac     3420

caagacatgt tttgatttat ggtgctggtg ttatcggttg tgaatatgct tctattttca     3480

gaggtatgga tgttaaggtt gacttgatta atacaagaga cagattatta gctttccttg     3540

atcaggaaat gtctgattcc ctttcctacc atttttggaa ctccggtgtc gtcatcagac     3600

acaacgagga atatgaaaag attgaaggtt gtgatgacgg cgttattatg caccttaagt     3660

ctggtaaaaa gttaaaagca gattgcttgt tatatgcaaa tggtagaacc ggtaacacag     3720

actccttggc tttacaaaac attggtttag aaaccgattc aagaggtcaa ttaaaggtca     3780

attcaatgta tcaaactgca caaccacacg tttacgcagt tggtgacgtt attggttacc     3840

cttcattggc atctgccgct tacgatcaag gtagaatcgc cgctcaagca cttgttaagg     3900

gtgaagcaac tgcacactta atcgaagata tccctaccgg tatctacact atcccagaaa     3960

tctcttctgt tggcaagact gaacaacaat taaccgcaat gaaggttcca tacgaagtcg     4020

gtcgtgccca gttcaagcat ttggctagag cacaaattgt tggtatgaat gttggtactt     4080

tgaaaatctt gtttcacaga gaaacaaagg aaatcttggg cattcactgt ttcggcgaaa     4140

gagctgcaga gattattcac atcggtcaag ccattatgga acaaaaaggc ggtggtaata     4200

ccattgaata tttcgttaat accaccttca actacccaac aatggccgaa gcatatagag     4260

tcgctgcttt aaacggttta aacagattgt tttaattaat ttattttact agtttatttt     4320

tgctcctgag aataggatta caaacactta aagtctttaa ttacaactat atataatatt     4380

ctgttggttt tcttgaattg gttcgctgcg attcatgcct cccattcacc aaaggtggag     4440

tgggaaataa cggttttact gcggtaatta gcagaggcaa gaacaggata cactttttga     4500

tgataaatct gtattatagt cgagcctatt taggaaatca aattttcttg tgtttacttt     4560

tcaaataaat aatgttcgaa aatttttact ttactccttc atttaactat accagacgtt     4620

atatcatcaa caccttctga ccatatacag ctcaagatgt ttaagagtct gttaaatttt     4680

ttcaatccat ttcatggagt accaggaggt gctacaaaag gaattcatag cctcatgaaa     4740

tcagccattt gcttttgttc aacgatcttt tgaaattgtt gttgttcttg gtagttaagt     4800

tgatccatct tggcttatgt tgtgtgtatg ttgtagttat tcttagtata ttcctgtcct     4860

gagtttagtg aaacataata tcgccttgaa atgaaaatgc tgaaattcgt cgacatacaa     4920

tttttcaaac tttttttttt tcttggtgca cggacatgtt tttaaaggaa gtactctata     4980

ccagttattc ttcacaaatt taattgctgg agaatagatc ttcaacgctt taataaagta     5040

gtttgtttgt caaggatggc gtcatacaaa gaaagatcag aatcacacac ttcccctgtt     5100

gctaggagac ttttctccat catggaggaa aagaagtcta acctttgtgc atcattggat     5160

attactgaaa ctgaaaagct tctctctatt ttggacacta ttggtcctta catctgtcta     5220

gttaaaacac acatcgatat tgtttctgat tttacgtatg aaggaactgt gttgcctttg     5280

aaggagcttg ccaagaaaca taattttatg atttttgaag atagaaaatt tgctgatatt     5340

ggtaacactg ttaaaaatca atataaatct ggtgtcttcc gtattgccga atgggctgac     5400

atcactaatg cacatggtgt aacgggtgca ggtattgttt ctggcttgaa ggaggcagcc     5460

caagaaacaa ccagtgaacc tagaggtttg ctaatgcttg ctgagttatc atcaaagggt     5520

tctttagcat atggtgaata tacagaaaaa acagtagaaa ttgctaaatc tgataaagag     5580

tttgtcattg gttttattgc gcaacacgat atgggcggta gagaagaagg ttttgactcc     5640

gc                                                                    5642


<210>  24
<211>  1401
<212>  DNA
<213>  Artificial

<220>
<223>  Codon optimized E. coli SthA gene

<400>  24
atgccacatt cctatgacta cgatgccatt gtcattggtt ccggtccagg tggtgaaggt       60

gctgcaatgg gcttagttaa gcagggtgct agagttgctg tcatcgaaag atatcaaaat      120

gttggtggtg gttgtactca ctggggtaca attccatcta aggcattgag acatgcagtt      180

tccagaatta ttgagtttaa ccaaaaccct ttatactctg atcattcaag attgttgaga      240

tcatcttttg ctgatatttt gaaccatgct gacaacgtca tcaaccaaca aactcgtatg      300

cgtcaaggct tctatgagag aaatcattgt gagattttac aaggtaacgc tagatttgtc      360

gatgagcata ctcttgcatt agactgtcca gacggttccg ttgagactct taccgctgaa      420

aaattcgtta ttgcttgtgg ttccagacca taccacccaa ccgatgtcga tttcactcac      480

cctcgtatct acgattccga ttctattttg tctatgcatc atgaaccaag acatgttttg      540

atttatggtg ctggtgttat cggttgtgaa tatgcttcta ttttcagagg tatggatgtt      600

aaggttgact tgattaatac aagagacaga ttattagctt tccttgatca ggaaatgtct      660

gattcccttt cctaccattt ttggaactcc ggtgtcgtca tcagacacaa cgaggaatat      720

gaaaagattg aaggttgtga tgacggcgtt attatgcacc ttaagtctgg taaaaagtta      780

aaagcagatt gcttgttata tgcaaatggt agaaccggta acacagactc cttggcttta      840

caaaacattg gtttagaaac cgattcaaga ggtcaattaa aggtcaattc aatgtatcaa      900

actgcacaac cacacgttta cgcagttggt gacgttattg gttacccttc attggcatct      960

gccgcttacg atcaaggtag aatcgccgct caagcacttg ttaagggtga agcaactgca     1020

cacttaatcg aagatatccc taccggtatc tacactatcc cagaaatctc ttctgttggc     1080

aagactgaac aacaattaac cgcaatgaag gttccatacg aagtcggtcg tgcccagttc     1140

aagcatttgg ctagagcaca aattgttggt atgaatgttg gtactttgaa aatcttgttt     1200

cacagagaaa caaaggaaat cttgggcatt cactgtttcg gcgaaagagc tgcagagatt     1260

attcacatcg gtcaagccat tatggaacaa aaaggcggtg gtaataccat tgaatatttc     1320

gttaatacca ccttcaacta cccaacaatg gccgaagcat atagagtcgc tgctttaaac     1380

ggtttaaaca gattgtttta a                                               1401


<210>  25
<211>  5304
<212>  DNA
<213>  Artificial

<220>
<223>  Codon-optimized E. coli SthA gene integration fragment

<400>  25
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttaaaca gcaatttgag gaaggaatag gagaaggaga agcaatttct     1200

aggaaagagc aaggtgtgca acagcatgct ctgaatgata ttttcagcaa tagttcagtt     1260

gaagaacctg ttggcgtatc tacatcactt cctacaaaca acaccacgaa ttgcgtccgt     1320

ggtgacgcaa ctacgaatgg cattgtcaat gccaatgcca gtgcacatac acgtgcaagt     1380

cccaccggtt ccctgcccgg ctatggtaga gacaagaagg acgataccgg catcgacatc     1440

aacagtttca acagcaatgc gtttggcgtc gacgcgtcga tggggctgcc gtatttggat     1500

ttggacgggc tagatttcga tatggatatg gatatggata tggatatgga gatgaatttg     1560

aatttagatt tgggtcttga tttggggttg gaattaaaag gggataacaa tgagggtttt     1620

cctgttgatt taaacaatgg acgtgggagg tgattgattt aacctgatcc aaaaggggta     1680

tgtctatttt ttagagtgtg tctttgtgtc aaattatggt agaatgtgta aagtagtata     1740

aactttcctc tcaaatgacg aggtttaaaa caccccccgg gtgagccgag ccgagaatgg     1800

ggcaattgtt caatgtgaaa tagaagtatc gagtgagaaa cttgggtgtt ggccagccaa     1860

gggggaagga aaatggcgcg aatgctcagg tgagattgtt ttggaattgg gtgaagcgag     1920

gaaatgagcg acccggaggt tgtgacttta gtggcggagg aggaacggga ggaaaaggcc     1980

aagagggaaa gtgtatataa gggggagcaa tttgccaacc aggatagaat tggatgagtt     2040

ataattctac tgtatttatt gtataattta tttctccttt tatatcaaac acattacaaa     2100

acacacaaaa catacaaaca tacacagcta gcaaaggcgc gccatctaat agtttaatca     2160

cagcttatag tctactatag ttttcttttt taaacattgt tgtattttgt cccccccctc     2220

taattgatga tgattatcct ataagaatcc aataaaacga tggaaactaa taccctctcc     2280

tttgtcatgt ggtctttagt atttcttgaa cattggctct gatttctcga ctttatagtc     2340

ctattaaaat cgctgttagt tctcgatcgt tgtatctcgt ttcttgtctc tttggtggat     2400

gattttgcgt gcgaacatgt ttttttccct ttctctcacc atcatcgtgt agttcttgtc     2460

accatccccc ccaccccttc cttctctcat tgattctata agagcttatc cacagaggtg     2520

cagtaacgag gtagtttaac cttcgagtgg atcaaaatgt cacacaggcc tcgacatttg     2580

ctgcaacggc aacatcaatg tccacgttta cacacctaca tttatatcta tatttatatt     2640

tatatttatt tatttatgct acttagcttc tatagttagt taatgcactc acgatattca     2700

aaattgacac ccttcaacta ctccctacta ttgtctacta ctgtctacta ctcctcttta     2760

ctatagctgc tcccaatagg ctccaccaat aggctctgtc aatacatttt gcgccgccac     2820

ctttcaggtt gtgtcactcc tgaaggacca tattgggtaa tcgtgcaatt tctggaagag     2880

agtccgcgag aagtgaggcc cccactgtaa atcctcgagg gggcatggag tatggggcat     2940

ggaggatgga ggatgggggg gggggggggg aaaataggta gcgaaaggac ccgctatcac     3000

cccacccgga gaactcgttg ccgggaagtc atatttcgac actccgggga gtctataaaa     3060

ggcgggtttt gtcttttgcc agttgatgtt gctgagagga cttgtttgcc gtttcttccg     3120

atttaacagt atagaatcaa ccactgttaa ttatacacgt tatactaaca caacaaaaac     3180

aaaaacaacg acaacaacaa caacatctag aatgccacat tcctatgact acgatgccat     3240

tgtcattggt tccggtccag gtggtgaagg tgctgcaatg ggcttagtta agcagggtgc     3300

tagagttgct gtcatcgaaa gatatcaaaa tgttggtggt ggttgtactc actggggtac     3360

aattccatct aaggcattga gacatgcagt ttccagaatt attgagttta accaaaaccc     3420

tttatactct gatcattcaa gattgttgag atcatctttt gctgatattt tgaaccatgc     3480

tgacaacgtc atcaaccaac aaactcgtat gcgtcaaggc ttctatgaga gaaatcattg     3540

tgagatttta caaggtaacg ctagatttgt cgatgagcat actcttgcat tagactgtcc     3600

agacggttcc gttgagactc ttaccgctga aaaattcgtt attgcttgtg gttccagacc     3660

ataccaccca accgatgtcg atttcactca ccctcgtatc tacgattccg attctatttt     3720

gtctatgcat catgaaccaa gacatgtttt gatttatggt gctggtgtta tcggttgtga     3780

atatgcttct attttcagag gtatggatgt taaggttgac ttgattaata caagagacag     3840

attattagct ttccttgatc aggaaatgtc tgattccctt tcctaccatt tttggaactc     3900

cggtgtcgtc atcagacaca acgaggaata tgaaaagatt gaaggttgtg atgacggcgt     3960

tattatgcac cttaagtctg gtaaaaagtt aaaagcagat tgcttgttat atgcaaatgg     4020

tagaaccggt aacacagact ccttggcttt acaaaacatt ggtttagaaa ccgattcaag     4080

aggtcaatta aaggtcaatt caatgtatca aactgcacaa ccacacgttt acgcagttgg     4140

tgacgttatt ggttaccctt cattggcatc tgccgcttac gatcaaggta gaatcgccgc     4200

tcaagcactt gttaagggtg aagcaactgc acacttaatc gaagatatcc ctaccggtat     4260

ctacactatc ccagaaatct cttctgttgg caagactgaa caacaattaa ccgcaatgaa     4320

ggttccatac gaagtcggtc gtgcccagtt caagcatttg gctagagcac aaattgttgg     4380

tatgaatgtt ggtactttga aaatcttgtt tcacagagaa acaaaggaaa tcttgggcat     4440

tcactgtttc ggcgaaagag ctgcagagat tattcacatc ggtcaagcca ttatggaaca     4500

aaaaggcggt ggtaatacca ttgaatattt cgttaatacc accttcaact acccaacaat     4560

ggccgaagca tatagagtcg ctgctttaaa cggtttaaac agattgtttt aattaacatc     4620

tgaatgtaaa atgaacatta aaatgaatta ctaaacttta cgtctacttt acaatctata     4680

aactttgttt aatcatataa cgaaatacac taatacacaa tcctgtacgt atgtaatact     4740

tttatccatc aaggattgag aaaaaaaagt aatgattccc tgggccatta aaacttagac     4800

ccccaagctt ggataggtca ctctctattt tcgtttctcc cttccctgat agaagggtga     4860

tatgtaatta agaataatat ataattttat aataaaagcg gccgccaagt tagttagagc     4920

tagagttaac acatacacat tatcaaatgc atttattcct aatatcacac taaaacgtat     4980

tatataattt taatctttat agacttcata gcaccaattg gatttgcttt ctttcagaat     5040

accgcactta atctcaatgt acgtaacgta ggcaaaatct gtcgataagg atctgtatgc     5100

cgtaaacgga aactccaagc gcccagaaaa cttacattat attcttgcca gtttcatctc     5160

accagccagt cacagtttaa aaggtttgat tgcgtttctt gtttcgtcgg attcagtgct     5220

aattggtaac gcactgtacc gccacaccaa agcaaaaatg cagaaacaaa caacaatgag     5280

tgtatgttta ccaactttgg ccgc                                            5304


<210>  26
<211>  5676
<212>  DNA
<213>  Artificial

<220>
<223>  A. vinelandii Stha gene integration fragment

<400>  26
aactactatg tacactgtat aagtaaaaag acgatacccc cctcccactc tgggtgctac       60

ggtgtagatc tctccgtaaa cacaaaaagg cggctcagat gataattggg gtccgggcgc      120

aaccggaagg ggggagagag gggagcgatg gcttctcctc cggggggcta cgggagtttc      180

ctctttggga aggataaaga ggggatggat tgatacaaga ttctgagaac ctattacgat      240

gatgttcagt ggtattttgt cttttgttat ttaaagggag gggactttcc tcaatacctt      300

agttgtaaaa ttacgctatt atctttaacc ctttcttttg agcaataatt aaaaagagcg      360

gccgcgagtc catcggttcc tgtcagatgg gatactcttg acgtggaaaa ttcaaacaga      420

aaaaaaaccc caataatgaa aaataacact acgttatatc cgtggtatcc tctatcgtat      480

cgtatcgtag cgtatcgtag cgtaccgtat cacagtatag tctaatattc cgtatcttat      540

tgtatcctat cctattcgat cctattgtat ttcagtgcac cattttaatt tctattgcta      600

taatgtcctt attagttgcc actgtgaggt gaccaatgga cgagggcgag ccgttcagaa      660

gccgcgaagg gtgttcttcc catgaatttc ttaaggaggg cggctcagct ccgagagtga      720

ggcgagacgt ctcggtcagc gtatccccct tcctcggctt ttacaaatga tgcgctctta      780

atagtgtgtc gttatccttt tggcattgac gggggaggga aattgattga gcgcatccat      840

atttttgcgg actgctgagg acaatggtgg tttttccggg tggcgtgggc tacaaatgat      900

acgatggttt ttttcttttc ggagaaggcg tataaaaagg acacggagaa cccatttatt      960

ctaaaaacag ttgagcttct ttaattattt tttgatataa tattctatta ttatatattt     1020

tcttcccaat aaaacaaaat aaaacaaaac acagcaaaac acaaaaagct agcctgaaag     1080

ggaaccataa tgggtaagat cgcaccacat tagcgggctc gaagatggat cttgcgaatg     1140

ggtgacacca gtcataaggc ctcgttgtcc cagcatacct cccgcgctat ctaattgctt     1200

cgctctccat tgttcttggt aaacatcact ctggcttgat ggtgtcatct atgcccgcca     1260

agcctatcgg tctatggccc ggagtttgct ccgtcttcca attgcaatcg cacggaatcc     1320

gggatagaaa gaacgatacg cattcatacg attctcacgt tattggttgg tgaatcaaat     1380

gcacaacgaa cccaatcgcc ctggactcag cgtctaggcc ccccgtatgg ccgacgggga     1440

ctcagagcgt caatccacgt tgaagtcgag gttttggcag ttacagccct tgcaataagg     1500

tttttcggac agtctacttt gtcggcgcgc cttctgtctt tgattttctt atgttattca     1560

aaacatctgc cccaaaatct aacgattata tatattccta cgtataactg tatagctaat     1620

tattgattta tttgtacata aaaaccacat aaatgtaaaa gcaagaaaaa aaataactaa     1680

ggagaaggat caatatctca tttataatgc tcgccaaagc agcgtacgtg aattttaatc     1740

aagacatcaa caaatcttgc aacttggtta tatcgcttct tcacccactc acccgctttt     1800

ctacattgtt gaacacaaat atatacaggg gtatgtctca aggtcaagtg cagtttcaac     1860

agagactacc tcaaggtacc tcttcagaaa tgcagaactt cactcttgat cagattttct     1920

ccgaattaaa ggaggcctat tggtagttct ttccccctct caagctggcg tgaaatgcaa     1980

ccttacggcg tctacgttac tacaaggtcc agaaagtgta ggtattgcta ctatttttat     2040

tttttattgg ttctggagaa atgcagacag tcaatgaaca caactgtctc aatatgcatc     2100

tatgcacatg cacacacaca cacatcacag gtacccctac aaagagaggt ctcttgataa     2160

tgtttcatta ccacgtggca tccccccccc cccccccaat aaacaagtgg ccgagttccc     2220

ctgttgcaga ggaggacaaa aaaaccgctg gtgttggtac cattatgcag caactagcac     2280

aacaaacaac cgacccagac atacaaatca acaacacttc gccaaagaca ccctttccag     2340

ggaggatcca ctcccaacgt ctctccataa tgtctctgtt ggcccatgtc tctgtcgttg     2400

acaccgtaac cacaccaacc aacccgtcca ttgtactggg atggtcgtcc atagacacct     2460

ctccaacggg gaacacctca ttcgtaaacc gccaaggtta ccgttcctcc tgactcgccc     2520

cgttgttgat gctgcgcacc tgtggttgcc caacatggtt gtatatcgtg taaccacacc     2580

aacacatgtg cagcacatgt gtttaaaaga gtgtcatgga ggtggatcat gatggaagtg     2640

gactttacca cttgggaact gtctccactc ccgggaagaa aagacccggc gtatcacgcg     2700

gttgcctcaa tggggcaatt tggaaggaga aatataggga aaatcacgtc gctctcggac     2760

ggggaagagt tccagactat gagggggggg ggtggtatat aaagacagga gatgtccacc     2820

cccagagaga ggaagaagtt ggaactttag aagagagaga taactttccc cagtgtccat     2880

caatacacaa ccaaacacaa actctatatt tacacatata accccctctc tagaatggca     2940

gtctataact atgatgttgt tgtcattggt actggtccag ctggtgaagg tgctgctatg     3000

aatgctgtca aagctggcag aaaggttgct gtcgttgacg acagacctca agtcggtggt     3060

aactgtactc atcttggtac tatcccatcc aaggcattaa gacattcagt tagacagatc     3120

atgcagtata acaacaaccc attattcaga caaattggtg aacctagatg gttttctttc     3180

gcagacgttc ttaagtccgc tgaacaagtt atcgcaaagc aagtctcttc aagaaccggc     3240

tattacgcaa gaaatcgtat tgatactttc tttggcaccg cctcattctg tgatgaacat     3300

actatcgaag ttgtccactt gaatggtatg gttgaaacct tagttgctaa gcaattcgtt     3360

attgcaacag gttcaagacc atacagacca gctgacgtcg actttaccca cccaagaatc     3420

tacgattccg ataccattct ttccttgggt catacaccaa gacgtttgat tatctacggt     3480

gccggtgtca ttggctgtga gtacgcttca attttctccg gtttaggtgt tttagttgat     3540

ttgattgaca acagagatca gttgttgtcc tttttggatg atgaaatttc tgattctttg     3600

tcctatcact taagaaataa caacgttttg attagacaca acgaagaata cgaaagagtt     3660

gaaggtcttg ataatggtgt tatcttacac ttaaagtctg gtaaaaagat taaggcagat     3720

gcatttttgt ggtctaacgg tagaactggt aacactgata agttaggttt ggaaaacatt     3780

ggtttgaagg ctaatggcag aggtcaaatt caagttgatg agcattatcg tacagaagtc     3840

tccaatatct acgcagccgg tgacgtcatc ggttggccat ccttagcttc agcagcttat     3900

gatcaaggta gatctgctgc tggttctatt accgagaatg actcttggcg tttcgttgat     3960

gatgttccta ccggtatcta caccatccct gaaatttcct ctgttggtaa aaccgaaaga     4020

gagttgacac aagcaaaagt cccatacgag gttggtaaag cctttttcaa gggcatggct     4080

cgtgcacaaa ttgcagttga aaaagccggt atgttaaaga ttctttttca tagagagact     4140

ttagaaatct tgggtgtcca ctgcttcggt taccaagcat ctgaaattgt tcatattggt     4200

caagcaatta tgaaccaaaa gggcgaagca aatacattaa agtatttcat caacactaca     4260

ttcaattatc caactatggc tgaagcttat agagttgcag cctacgacgg tttaaacaga     4320

ttgttttaat taatttattt tactagttta tttttgctcc tgagaatagg attacaaaca     4380

cttaaagtct ttaattacaa ctatatataa tattctgttg gttttcttga attggttcgc     4440

tgcgattcat gcctcccatt caccaaaggt ggagtgggaa ataacggttt tactgcggta     4500

attagcagag gcaagaacag gatacacttt ttgatgataa atctgtatta tagtcgagcc     4560

tatttaggaa atcaaatttt cttgtgttta cttttcaaat aaataatgtt cgaaaatttt     4620

tactttactc cttcatttaa ctataccaga cgttatatca tcaacacctt ctgaccatat     4680

acagctcaag atgtttaaga gtctgttaaa ttttttcaat ccatttcatg gagtaccagg     4740

aggtgctaca aaaggaattc atagcctcat gaaatcagcc atttgctttt gttcaacgat     4800

cttttgaaat tgttgttgtt cttggtagtt aagttgatcc atcttggctt atgttgtgtg     4860

tatgttgtag ttattcttag tatattcctg tcctgagttt agtgaaacat aatatcgcct     4920

tgaaatgaaa atgctgaaat tcgtcgacat acaatttttc aaactttttt tttttcttgg     4980

tgcacggaca tgtttttaaa ggaagtactc tataccagtt attcttcaca aatttaattg     5040

ctggagaata gatcttcaac gctttaataa agtagtttgt ttgtcaagga tggcgtcata     5100

caaagaaaga tcagaatcac acacttcccc tgttgctagg agacttttct ccatcatgga     5160

ggaaaagaag tctaaccttt gtgcatcatt ggatattact gaaactgaaa agcttctctc     5220

tattttggac actattggtc cttacatctg tctagttaaa acacacatcg atattgtttc     5280

tgattttacg tatgaaggaa ctgtgttgcc tttgaaggag cttgccaaga aacataattt     5340

tatgattttt gaagatagaa aatttgctga tattggtaac actgttaaaa atcaatataa     5400

atctggtgtc ttccgtattg ccgaatgggc tgacatcact aatgcacatg gtgtaacggg     5460

tgcaggtatt gtttctggct tgaaggaggc agcccaagaa acaaccagtg aacctagagg     5520

tttgctaatg cttgctgagt tatcatcaaa gggttcttta gcatatggtg aatatacaga     5580

aaaaacagta gaaattgcta aatctgataa agagtttgtc attggtttta ttgcgcaaca     5640

cgatatgggc ggtagagaag aaggttttga ctccgc                               5676


<210>  27
<211>  1395
<212>  DNA
<213>  Azotobacter vinelandii

<400>  27
atggcagtct ataactatga tgttgttgtc attggtactg gtccagctgg tgaaggtgct       60

gctatgaatg ctgtcaaagc tggcagaaag gttgctgtcg ttgacgacag acctcaagtc      120

ggtggtaact gtactcatct tggtactatc ccatccaagg cattaagaca ttcagttaga      180

cagatcatgc agtataacaa caacccatta ttcagacaaa ttggtgaacc tagatggttt      240

tctttcgcag acgttcttaa gtccgctgaa caagttatcg caaagcaagt ctcttcaaga      300

accggctatt acgcaagaaa tcgtattgat actttctttg gcaccgcctc attctgtgat      360

gaacatacta tcgaagttgt ccacttgaat ggtatggttg aaaccttagt tgctaagcaa      420

ttcgttattg caacaggttc aagaccatac agaccagctg acgtcgactt tacccaccca      480

agaatctacg attccgatac cattctttcc ttgggtcata caccaagacg tttgattatc      540

tacggtgccg gtgtcattgg ctgtgagtac gcttcaattt tctccggttt aggtgtttta      600

gttgatttga ttgacaacag agatcagttg ttgtcctttt tggatgatga aatttctgat      660

tctttgtcct atcacttaag aaataacaac gttttgatta gacacaacga agaatacgaa      720

agagttgaag gtcttgataa tggtgttatc ttacacttaa agtctggtaa aaagattaag      780

gcagatgcat ttttgtggtc taacggtaga actggtaaca ctgataagtt aggtttggaa      840

aacattggtt tgaaggctaa tggcagaggt caaattcaag ttgatgagca ttatcgtaca      900

gaagtctcca atatctacgc agccggtgac gtcatcggtt ggccatcctt agcttcagca      960

gcttatgatc aaggtagatc tgctgctggt tctattaccg agaatgactc ttggcgtttc     1020

gttgatgatg ttcctaccgg tatctacacc atccctgaaa tttcctctgt tggtaaaacc     1080

gaaagagagt tgacacaagc aaaagtccca tacgaggttg gtaaagcctt tttcaagggc     1140

atggctcgtg cacaaattgc agttgaaaaa gccggtatgt taaagattct ttttcataga     1200

gagactttag aaatcttggg tgtccactgc ttcggttacc aagcatctga aattgttcat     1260

attggtcaag caattatgaa ccaaaagggc gaagcaaata cattaaagta tttcatcaac     1320

actacattca attatccaac tatggctgaa gcttatagag ttgcagccta cgacggttta     1380

aacagattgt tttaa                                                      1395


<210>  28
<211>  5298
<212>  DNA
<213>  Artificial

<220>
<223>  A. vinelandii SthA gene integration fragment

<400>  28
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttaaaca gcaatttgag gaaggaatag gagaaggaga agcaatttct     1200

aggaaagagc aaggtgtgca acagcatgct ctgaatgata ttttcagcaa tagttcagtt     1260

gaagaacctg ttggcgtatc tacatcactt cctacaaaca acaccacgaa ttgcgtccgt     1320

ggtgacgcaa ctacgaatgg cattgtcaat gccaatgcca gtgcacatac acgtgcaagt     1380

cccaccggtt ccctgcccgg ctatggtaga gacaagaagg acgataccgg catcgacatc     1440

aacagtttca acagcaatgc gtttggcgtc gacgcgtcga tggggctgcc gtatttggat     1500

ttggacgggc tagatttcga tatggatatg gatatggata tggatatgga gatgaatttg     1560

aatttagatt tgggtcttga tttggggttg gaattaaaag gggataacaa tgagggtttt     1620

cctgttgatt taaacaatgg acgtgggagg tgattgattt aacctgatcc aaaaggggta     1680

tgtctatttt ttagagtgtg tctttgtgtc aaattatggt agaatgtgta aagtagtata     1740

aactttcctc tcaaatgacg aggtttaaaa caccccccgg gtgagccgag ccgagaatgg     1800

ggcaattgtt caatgtgaaa tagaagtatc gagtgagaaa cttgggtgtt ggccagccaa     1860

gggggaagga aaatggcgcg aatgctcagg tgagattgtt ttggaattgg gtgaagcgag     1920

gaaatgagcg acccggaggt tgtgacttta gtggcggagg aggaacggga ggaaaaggcc     1980

aagagggaaa gtgtatataa gggggagcaa tttgccaacc aggatagaat tggatgagtt     2040

ataattctac tgtatttatt gtataattta tttctccttt tatatcaaac acattacaaa     2100

acacacaaaa catacaaaca tacacagcta gcaaaggcgc gccatctaat agtttaatca     2160

cagcttatag tctactatag ttttcttttt taaacattgt tgtattttgt cccccccctc     2220

taattgatga tgattatcct ataagaatcc aataaaacga tggaaactaa taccctctcc     2280

tttgtcatgt ggtctttagt atttcttgaa cattggctct gatttctcga ctttatagtc     2340

ctattaaaat cgctgttagt tctcgatcgt tgtatctcgt ttcttgtctc tttggtggat     2400

gattttgcgt gcgaacatgt ttttttccct ttctctcacc atcatcgtgt agttcttgtc     2460

accatccccc ccaccccttc cttctctcat tgattctata agagcttatc cacagaggtg     2520

cagtaacgag gtagtttaac cttcgagtgg atcaaaatgt cacacaggcc tcgacatttg     2580

ctgcaacggc aacatcaatg tccacgttta cacacctaca tttatatcta tatttatatt     2640

tatatttatt tatttatgct acttagcttc tatagttagt taatgcactc acgatattca     2700

aaattgacac ccttcaacta ctccctacta ttgtctacta ctgtctacta ctcctcttta     2760

ctatagctgc tcccaatagg ctccaccaat aggctctgtc aatacatttt gcgccgccac     2820

ctttcaggtt gtgtcactcc tgaaggacca tattgggtaa tcgtgcaatt tctggaagag     2880

agtccgcgag aagtgaggcc cccactgtaa atcctcgagg gggcatggag tatggggcat     2940

ggaggatgga ggatgggggg gggggggggg aaaataggta gcgaaaggac ccgctatcac     3000

cccacccgga gaactcgttg ccgggaagtc atatttcgac actccgggga gtctataaaa     3060

ggcgggtttt gtcttttgcc agttgatgtt gctgagagga cttgtttgcc gtttcttccg     3120

atttaacagt atagaatcaa ccactgttaa ttatacacgt tatactaaca caacaaaaac     3180

aaaaacaacg acaacaacaa caacatctag aatggcagtc tataactatg atgttgttgt     3240

cattggtact ggtccagctg gtgaaggtgc tgctatgaat gctgtcaaag ctggcagaaa     3300

ggttgctgtc gttgacgaca gacctcaagt cggtggtaac tgtactcatc ttggtactat     3360

cccatccaag gcattaagac attcagttag acagatcatg cagtataaca acaacccatt     3420

attcagacaa attggtgaac ctagatggtt ttctttcgca gacgttctta agtccgctga     3480

acaagttatc gcaaagcaag tctcttcaag aaccggctat tacgcaagaa atcgtattga     3540

tactttcttt ggcaccgcct cattctgtga tgaacatact atcgaagttg tccacttgaa     3600

tggtatggtt gaaaccttag ttgctaagca attcgttatt gcaacaggtt caagaccata     3660

cagaccagct gacgtcgact ttacccaccc aagaatctac gattccgata ccattctttc     3720

cttgggtcat acaccaagac gtttgattat ctacggtgcc ggtgtcattg gctgtgagta     3780

cgcttcaatt ttctccggtt taggtgtttt agttgatttg attgacaaca gagatcagtt     3840

gttgtccttt ttggatgatg aaatttctga ttctttgtcc tatcacttaa gaaataacaa     3900

cgttttgatt agacacaacg aagaatacga aagagttgaa ggtcttgata atggtgttat     3960

cttacactta aagtctggta aaaagattaa ggcagatgca tttttgtggt ctaacggtag     4020

aactggtaac actgataagt taggtttgga aaacattggt ttgaaggcta atggcagagg     4080

tcaaattcaa gttgatgagc attatcgtac agaagtctcc aatatctacg cagccggtga     4140

cgtcatcggt tggccatcct tagcttcagc agcttatgat caaggtagat ctgctgctgg     4200

ttctattacc gagaatgact cttggcgttt cgttgatgat gttcctaccg gtatctacac     4260

catccctgaa atttcctctg ttggtaaaac cgaaagagag ttgacacaag caaaagtccc     4320

atacgaggtt ggtaaagcct ttttcaaggg catggctcgt gcacaaattg cagttgaaaa     4380

agccggtatg ttaaagattc tttttcatag agagacttta gaaatcttgg gtgtccactg     4440

cttcggttac caagcatctg aaattgttca tattggtcaa gcaattatga accaaaaggg     4500

cgaagcaaat acattaaagt atttcatcaa cactacattc aattatccaa ctatggctga     4560

agcttataga gttgcagcct acgacggttt aaacagattg ttttaattaa catctgaatg     4620

taaaatgaac attaaaatga attactaaac tttacgtcta ctttacaatc tataaacttt     4680

gtttaatcat ataacgaaat acactaatac acaatcctgt acgtatgtaa tacttttatc     4740

catcaaggat tgagaaaaaa aagtaatgat tccctgggcc attaaaactt agacccccaa     4800

gcttggatag gtcactctct attttcgttt ctcccttccc tgatagaagg gtgatatgta     4860

attaagaata atatataatt ttataataaa agcggccgcc aagttagtta gagctagagt     4920

taacacatac acattatcaa atgcatttat tcctaatatc acactaaaac gtattatata     4980

attttaatct ttatagactt catagcacca attggatttg ctttctttca gaataccgca     5040

cttaatctca atgtacgtaa cgtaggcaaa atctgtcgat aaggatctgt atgccgtaaa     5100

cggaaactcc aagcgcccag aaaacttaca ttatattctt gccagtttca tctcaccagc     5160

cagtcacagt ttaaaaggtt tgattgcgtt tcttgtttcg tcggattcag tgctaattgg     5220

taacgcactg taccgccaca ccaaagcaaa aatgcagaaa caaacaacaa tgagtgtatg     5280

tttaccaact ttggccgc                                                   5298


<210>  29
<211>  6139
<212>  DNA
<213>  Artificial

<220>
<223>  S. cerevisiae SthA gene integration fragment

<400>  29
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttaaaca gcaatttgag gaaggaatag gagaaggaga agcaatttct     1200

aggaaagagc aaggtgtgca acagcatgct ctgaatgata ttttcagcaa tagttcagtt     1260

gaagaacctg ttggcgtatc tacatcactt cctacaaaca acaccacgaa ttgcgtccgt     1320

ggtgacgcaa ctacgaatgg cattgtcaat gccaatgcca gtgcacatac acgtgcaagt     1380

cccaccggtt ccctgcccgg ctatggtaga gacaagaagg acgataccgg catcgacatc     1440

aacagtttca acagcaatgc gtttggcgtc gacgcgtcga tggggctgcc gtatttggat     1500

ttggacgggc tagatttcga tatggatatg gatatggata tggatatgga gatgaatttg     1560

aatttagatt tgggtcttga tttggggttg gaattaaaag gggataacaa tgagggtttt     1620

cctgttgatt taaacaatgg acgtgggagg tgattgattt aacctgatcc aaaaggggta     1680

tgtctatttt ttagagtgtg tctttgtgtc aaattatggt agaatgtgta aagtagtata     1740

aactttcctc tcaaatgacg aggtttaaaa caccccccgg gtgagccgag ccgagaatgg     1800

ggcaattgtt caatgtgaaa tagaagtatc gagtgagaaa cttgggtgtt ggccagccaa     1860

gggggaagga aaatggcgcg aatgctcagg tgagattgtt ttggaattgg gtgaagcgag     1920

gaaatgagcg acccggaggt tgtgacttta gtggcggagg aggaacggga ggaaaaggcc     1980

aagagggaaa gtgtatataa gggggagcaa tttgccaacc aggatagaat tggatgagtt     2040

ataattctac tgtatttatt gtataattta tttctccttt tatatcaaac acattacaaa     2100

acacacaaaa catacaaaca tacacagcta gcatggatgg tcctaacttc gcccatcaag     2160

gcggtagatc ccaaagaact accgagttgt actcatgtgc acgttgccgt aagcttaaga     2220

agaaatgtgg taaacaaatc ccaacttgtg caaactgtga taagaacggt gcacattgtt     2280

cttatcctgg tagagctcct agacgtacca agaaggagtt ggctgatgca atgcttagag     2340

gtgaatatgt tccagttaaa cgtaacaaga aagtcggcaa atctccttta tctactaagt     2400

ctatgccaaa ctcctcttct ccattatccg ctaacggtgc aatcactcct ggtttttctc     2460

catatgaaaa cgatgacgcc cacaagatga agcagttaaa gccatctgat ccaattaact     2520

tagtcatggg tgcatctcca aattcctccg agggcgtttc ctctttgatt tccgttttaa     2580

cctcattgaa cgacaattcc aatccttctt ctcacttgtc ctctaacgaa aattccatga     2640

tcccttctcg ttcccttcca gcatccgttc aacaatcatc tacaacttct tcctttggtg     2700

gttataacac cccatcacca ttgatttcct ctcacgttcc agccaatgca caagcagtcc     2760

cattacaaaa caacaacaga aacacctcta acggtgacaa tggttctaac gttaatcatg     2820

acaacaataa tggttccacc aacacaccac aattatcctt gactccatac gctaataact     2880

ctgctcctaa cggcaaattc gattccgtcc ctgtcgatgc ttcttccatc gagtttgaga     2940

caatgtcttg ttgttttaag ggtggcagaa ctacttcttg ggttagagaa gatggttctt     3000

ttaagtctat tgacagatca ttattggaca gattcatcgc agcttacttc aagcacaacc     3060

acagattgtt cccaatgatt gataagattg cattcttgaa tgatgctgct actattaccg     3120

atttcgaaag attgtacgat aacaagaact atccagattc ttttgttttc aaggtttaca     3180

tgattatggc aattggctgt actacattac aaagagctgg catggtttcc caagacgaag     3240

aatgtttgtc tgaacatttg gctttccttg ctatgaagaa gtttagatct gtcattatct     3300

tacaagatat cgaaactgtt agatgcttgt tgttattagg tatctactca ttctttgaac     3360

caaagggttc ttcctcttgg actatttcag gcattatcat gcgtcttact attggtttgg     3420

gtttgaatag agaattgact gctaagaaat tgaagtctat gtcagcctta gaagcagaag     3480

caagatatag agttttctgg tctgcttatt gttttgaaag attggtctgt acatccttgg     3540

gtagaatttc cggtattgac gacgaagata ttactgttcc attaccaaga gcattgtatg     3600

tcgatgagag agatgatttg gaaatgacca agttaatgat ctccttaaga aagatgggtg     3660

gtagaatcta caagcaagtc cattctgttt ccgctggtag acaaaagttg accatcgaac     3720

agaagcagga gattatctct ggtttgagaa aagaacttga cgaaatctac tccagagaat     3780

ccgaaagaag aaagttaaag aagtctcaaa tggaccaagt cgaaagagaa aacaattcaa     3840

caactaatgt tatttccttt cactcatctg agatttggtt agctatgaga tactctcaat     3900

tgcaaatctt gttgtataga ccatccgccc ttatgccaaa accacctatt gattctttgt     3960

caacccttgg tgaattttgt ttacaagcat ggaaacacac ttacacattg tacaagaaaa     4020

gattgttacc attgaattgg attaccttat tcagaacttt aactatttgt aacacaatct     4080

tatactgttt atgccaatgg tccattgact taattgaatc taagattgaa atccaacagt     4140

gtgttgaaat cttgcgtcat tttggtgaaa gatggatttt cgccatgaga tgtgctgatg     4200

ttttccaaaa catttcaaat accattttag acatctccct ttcccatggt aaagttccaa     4260

acatggatca attaaccaga gagttattcg gtgcatctga ctcctaccaa gacatcttag     4320

acgaaaacaa tgttgatgtt tcttgggtcg ataagttggt ctaaggcgcg ccatctaata     4380

gtttaatcac agcttatagt ctactatagt tttctttttt aaacattgtt gtattttgtc     4440

ccccccctct aattgatgat gattatccta taagaatcca ataaaacgat ggaaactaat     4500

accctctcct ttgtcatgtg gtctttagta tttcttgaac attggctctg atttctcgac     4560

tttatagtcc tattaaaatc gctgttagtt ctcgatcgtt gtatctcgtt tcttgtctct     4620

ttggtggatg attttgcgtg cgaacatgtt tttttccctt tctctcacca tcatcgtgta     4680

gttcttgtca ccatcccccc caccccttcc ttctctcatt gattctataa gagcttatcc     4740

acagaggtgc agtaacgagg tagtttaacc ttcgagtgga tcaaaatgtc acacaggcct     4800

cgacatttgc tgcaacggca acatcaatgt ccacgtttac acacctacat ttatatctat     4860

atttatattt atatttattt atttatgcta cttagcttct atagttagtt aatgcactca     4920

cgatattcaa aattgacacc cttcaactac tccctactat tgtctactac tgtctactac     4980

tcctctttac tatagctgct cccaataggc tccaccaata ggctctgtca atacattttg     5040

cgccgccacc tttcaggttg tgtcactcct gaaggaccat attgggtaat cgtgcaattt     5100

ctggaagaga gtccgcgaga agtgaggccc ccactgtaaa tcctcgaggg ggcatggagt     5160

atggggcatg gaggatggag gatggggggg ggggggggga aaataggtag cgaaaggacc     5220

cgctatcacc ccacccggag aactcgttgc cgggaagtca tatttcgaca ctccggggag     5280

tctataaaag gcgggttttg tcttttgcca gttgatgttg ctgagaggac ttgtttgccg     5340

tttcttccga tttaacagta tagaatcaac cactgttaat tatacacgtt atactaacac     5400

aacaaaaaca aaaacaacga caacaacaac aacatctaga taattaatta acatctgaat     5460

gtaaaatgaa cattaaaatg aattactaaa ctttacgtct actttacaat ctataaactt     5520

tgtttaatca tataacgaaa tacactaata cacaatcctg tacgtatgta atacttttat     5580

ccatcaagga ttgagaaaaa aaagtaatga ttccctgggc cattaaaact tagaccccca     5640

agcttggata ggtcactctc tattttcgtt tctcccttcc ctgatagaag ggtgatatgt     5700

aattaagaat aatatataat tttataataa aagcggccgc caagttagtt agagctagag     5760

ttaacacata cacattatca aatgcattta ttcctaatat cacactaaaa cgtattatat     5820

aattttaatc tttatagact tcatagcacc aattggattt gctttctttc agaataccgc     5880

acttaatctc aatgtacgta acgtaggcaa aatctgtcga taaggatctg tatgccgtaa     5940

acggaaactc caagcgccca gaaaacttac attatattct tgccagtttc atctcaccag     6000

ccagtcacag tttaaaaggt ttgattgcgt ttcttgtttc gtcggattca gtgctaattg     6060

gtaacgcact gtaccgccac accaaagcaa aaatgcagaa acaaacaaca atgagtgtat     6120

gtttaccaac tttggccgc                                                  6139


<210>  30
<211>  2232
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  30
atggatggtc ctaacttcgc ccatcaaggc ggtagatccc aaagaactac cgagttgtac       60

tcatgtgcac gttgccgtaa gcttaagaag aaatgtggta aacaaatccc aacttgtgca      120

aactgtgata agaacggtgc acattgttct tatcctggta gagctcctag acgtaccaag      180

aaggagttgg ctgatgcaat gcttagaggt gaatatgttc cagttaaacg taacaagaaa      240

gtcggcaaat ctcctttatc tactaagtct atgccaaact cctcttctcc attatccgct      300

aacggtgcaa tcactcctgg tttttctcca tatgaaaacg atgacgccca caagatgaag      360

cagttaaagc catctgatcc aattaactta gtcatgggtg catctccaaa ttcctccgag      420

ggcgtttcct ctttgatttc cgttttaacc tcattgaacg acaattccaa tccttcttct      480

cacttgtcct ctaacgaaaa ttccatgatc ccttctcgtt cccttccagc atccgttcaa      540

caatcatcta caacttcttc ctttggtggt tataacaccc catcaccatt gatttcctct      600

cacgttccag ccaatgcaca agcagtccca ttacaaaaca acaacagaaa cacctctaac      660

ggtgacaatg gttctaacgt taatcatgac aacaataatg gttccaccaa cacaccacaa      720

ttatccttga ctccatacgc taataactct gctcctaacg gcaaattcga ttccgtccct      780

gtcgatgctt cttccatcga gtttgagaca atgtcttgtt gttttaaggg tggcagaact      840

acttcttggg ttagagaaga tggttctttt aagtctattg acagatcatt attggacaga      900

ttcatcgcag cttacttcaa gcacaaccac agattgttcc caatgattga taagattgca      960

ttcttgaatg atgctgctac tattaccgat ttcgaaagat tgtacgataa caagaactat     1020

ccagattctt ttgttttcaa ggtttacatg attatggcaa ttggctgtac tacattacaa     1080

agagctggca tggtttccca agacgaagaa tgtttgtctg aacatttggc tttccttgct     1140

atgaagaagt ttagatctgt cattatctta caagatatcg aaactgttag atgcttgttg     1200

ttattaggta tctactcatt ctttgaacca aagggttctt cctcttggac tatttcaggc     1260

attatcatgc gtcttactat tggtttgggt ttgaatagag aattgactgc taagaaattg     1320

aagtctatgt cagccttaga agcagaagca agatatagag ttttctggtc tgcttattgt     1380

tttgaaagat tggtctgtac atccttgggt agaatttccg gtattgacga cgaagatatt     1440

actgttccat taccaagagc attgtatgtc gatgagagag atgatttgga aatgaccaag     1500

ttaatgatct ccttaagaaa gatgggtggt agaatctaca agcaagtcca ttctgtttcc     1560

gctggtagac aaaagttgac catcgaacag aagcaggaga ttatctctgg tttgagaaaa     1620

gaacttgacg aaatctactc cagagaatcc gaaagaagaa agttaaagaa gtctcaaatg     1680

gaccaagtcg aaagagaaaa caattcaaca actaatgtta tttcctttca ctcatctgag     1740

atttggttag ctatgagata ctctcaattg caaatcttgt tgtatagacc atccgccctt     1800

atgccaaaac cacctattga ttctttgtca acccttggtg aattttgttt acaagcatgg     1860

aaacacactt acacattgta caagaaaaga ttgttaccat tgaattggat taccttattc     1920

agaactttaa ctatttgtaa cacaatctta tactgtttat gccaatggtc cattgactta     1980

attgaatcta agattgaaat ccaacagtgt gttgaaatct tgcgtcattt tggtgaaaga     2040

tggattttcg ccatgagatg tgctgatgtt ttccaaaaca tttcaaatac cattttagac     2100

atctcccttt cccatggtaa agttccaaac atggatcaat taaccagaga gttattcggt     2160

gcatctgact cctaccaaga catcttagac gaaaacaatg ttgatgtttc ttgggtcgat     2220

aagttggtct aa                                                         2232


<210>  31
<211>  506
<212>  PRT
<213>  Issatchenkia orientalis

<400>  31

Met Gly Val Gln Phe Ile Glu Asn Thr Ile Ile Val Val Phe Gly Ala 
1               5                   10                  15      


Ser Gly Asp Leu Ala Lys Lys Lys Thr Phe Pro Ala Leu Phe Gly Leu 
            20                  25                  30          


Phe Arg Glu Gly Gln Leu Ser Glu Thr Thr Lys Ile Ile Gly Phe Ala 
        35                  40                  45              


Arg Ser Lys Leu Ser Asn Asp Asp Leu Arg Asn Arg Ile Lys Pro Tyr 
    50                  55                  60                  


Leu Lys Leu Asn Lys Arg Thr Asp Ala Glu Arg Gln Ser Leu Glu Lys 
65                  70                  75                  80  


Phe Leu Gln Ile Leu Glu Tyr His Gln Ser Asn Tyr Asp Asp Ser Glu 
                85                  90                  95      


Gly Phe Glu Lys Leu Glu Lys Leu Ile Asn Lys Tyr Asp Asp Glu Ala 
            100                 105                 110         


Asn Val Lys Glu Ser His Arg Leu Tyr Tyr Leu Ala Leu Pro Pro Ser 
        115                 120                 125             


Val Phe Thr Thr Val Ala Thr Met Leu Lys Lys His Cys His Pro Gly 
    130                 135                 140                 


Asp Ser Gly Ile Ala Arg Leu Ile Val Glu Lys Pro Phe Gly His Asp 
145                 150                 155                 160 


Leu Ser Ser Ser Arg Glu Leu Gln Lys Ser Leu Ala Pro Leu Trp Asn 
                165                 170                 175     


Glu Asp Glu Leu Phe Arg Ile Asp His Tyr Leu Gly Lys Glu Met Val 
            180                 185                 190         


Lys Asn Leu Ile Pro Leu Arg Phe Ser Asn Thr Phe Leu Ser Ser Ser 
        195                 200                 205             


Trp Asn Asn Gln Phe Ile Asp Thr Ile Gln Ile Thr Phe Lys Glu Asn 
    210                 215                 220                 


Phe Gly Thr Glu Gly Arg Gly Gly Tyr Phe Asp Ser Ile Gly Ile Ile 
225                 230                 235                 240 


Arg Asp Val Ile Gln Asn His Leu Leu Gln Val Leu Thr Ile Val Leu 
                245                 250                 255     


Met Glu Lys Pro Ala Asp Phe Asn Gly Glu Ser Ile Arg Asp Glu Lys 
            260                 265                 270         


Val Lys Val Leu Lys Ala Ile Glu Gln Ile Asp Phe Asn Asn Val Leu 
        275                 280                 285             


Val Gly Gln Tyr Asp Lys Ser Glu Asp Gly Ser Lys Pro Gly Tyr Leu 
    290                 295                 300                 


Asp Asp Asp Thr Val Asn Pro Asp Ser Lys Ala Val Thr Tyr Ala Ala 
305                 310                 315                 320 


Leu Val Leu Asn Val Ala Asn Glu Arg Trp Asn Asn Val Pro Ile Ile 
                325                 330                 335     


Leu Lys Ala Gly Lys Ala Leu Asn Gln Ser Lys Val Glu Ile Arg Ile 
            340                 345                 350         


Gln Phe Lys Pro Val Glu Asn Gly Ile Phe Lys Asn Ser Ala Arg Asn 
        355                 360                 365             


Glu Leu Val Ile Arg Ile Gln Pro Asn Glu Ala Met Tyr Leu Lys Met 
    370                 375                 380                 


Asn Ile Lys Val Pro Gly Val Ser Asn Gln Val Ser Ile Ser Glu Met 
385                 390                 395                 400 


Asp Leu Thr Tyr Lys Asn Arg Tyr Ser Ser Glu Phe Tyr Ile Pro Glu 
                405                 410                 415     


Ala Tyr Glu Ser Leu Ile Lys Asp Ala Leu Met Asp Asp His Ser Asn 
            420                 425                 430         


Phe Val Arg Asp Asp Glu Leu Asp Ile Ser Trp Ala Leu Phe Thr Pro 
        435                 440                 445             


Leu Leu Glu His Ile Glu Gly Pro Asp Gly Pro Thr Pro Thr Lys Tyr 
    450                 455                 460                 


Pro Tyr Gly Ser Arg Gly Pro Lys Glu Ile Asp Glu Phe Leu Arg Asn 
465                 470                 475                 480 


His Gly Tyr Val Lys Glu Pro Arg Glu Asn Tyr Gln Trp Pro Leu Thr 
                485                 490                 495     


Thr Pro Lys Glu Leu Asn Ser Ser Lys Phe 
            500                 505     


<210>  32
<211>  3435
<212>  DNA
<213>  Artificial

<220>
<223>  Mutated L. mexicana FRD gene

<400>  32
atggctgatg gcaaaacctc tgcatcagtt gttgctgttg atgctgaacg tgccgctaag       60

gaaagagatg cagcagctag agctatgttg caaggtggtg gtgtctctcc tgctggcaag      120

gcacaattgt tgaaaaaggg tttggttcac actgttccat ataccttaaa ggttgtcgtc      180

gcagatccaa aggaaatgga gaaggcaact gctgacgcag aagaggtttt acaagctgca      240

tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa actcagaagt ttcaagagtc      300

aataggttgg cagttggtga ggaacatcaa atgtctgaaa cattgaaaca cgtcatggcc      360

tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg acccagcagt tggtccatta      420

gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg ttccagccga aagagttaat      480

gatttgttat ccaaatgtac ccttaatgca tctttttcaa ttgatatgtc cagaggtatg      540

attgcaagga agcatccaga cgccatgttg gatttgggtg gtgtcaacaa gggttatggt      600

atcgactaca ttgttgaaca cttaaactct ttgggttatg atgatgtctt tttcggacgg      660

ggtggtgatg ttagagcatc cggcaaaaac cagttatctc aaccttgggc tgttggtatt      720

gttagaccac ctgccttggc cgacattaga actgttgtcc cagaggacaa aagatccttt      780

atccgtgtcg tcagattgaa caacgaagct attgctacct ctggtgatta tgagaatttg      840

gttgaaggtc ctggttctaa ggtttactct tccaccttca atccaacttc caaaaacttg      900

ttggaaccta ccgaagcagg tatggctcaa gtttctgtca agtgttgctc atgtatctac      960

gctgatgctt tagcaacagc agctttgttg aaaaacgatc ctgctgccgt tagaaggatc     1020

ttagataact ggagatatgt cagagatact gttactgact acaccactta cacaagggaa     1080

ggtgaaagag ttgctaagat gttggaaatt gctaccgaag atgctgaaat gagagcaaag     1140

agaatcaagg gctctttacc agcaagagtt atcattgttg gtggtggttt ggccggttgt     1200

tccgcagcta tcgaagcagc taactgtggc gcccacgtca tcttgttaga aaaggaacca     1260

aagttaggtg gtaactctgc aaaggctacc tccggtatca acgcctgggg tactagagca     1320

caagcaaaac aaggtgtcat ggacggcggc aagtttttcg aaagagatac ccatagatcc     1380

ggcaagggtg gtaattgcga tccatgcctt gttaagactt tgtccgttaa gtcctctgat     1440

gcagttaagt ggttatctga attaggtgtt ccattgactg ttttgtctca attaggtggt     1500

gcttcaagga aacgttgtca ccgtgcacca gataagtctg atggtacacc agtcccagtt     1560

ggtttcacca ttatgaaaac ccttgaaaac cacattgtca acgatttgtc cagacatgtt     1620

acagttatga caggtattac cgtcacagct ttagaatcta catcaagagt cagacctgat     1680

ggtgttttag tcaagcatgt tactggtgtt cacttgattc aggcatctgg tcaatctatg     1740

gttttgaatg cagacgctgt tatcttagct actggtggtt tctccaatga tcatacccca     1800

aactcccttt tacaacaata cgccccacag ttgtcatctt ttccaacaac caatggtgtc     1860

tgggcaactg gcgatggtgt taagatggct tccaagttgg gtgtcgcctt agttgatatg     1920

gataaggtcc aattacatcc taccggcttg ttagacccaa aagatccatc taatagaacc     1980

aagtatcttg gtccagaggc cttaagaggt tccggcggtg tcttgttaaa caaaaacggt     2040

gaaagatttg ttaatgaatt agacttaaga tctgttgtct ctcaagctat catcgcacaa     2100

gataatgagt acccaggctc tggtggttcc aagttcgcat actgtgtttt gaacgaaact     2160

gcagcaaagt tattcggcaa aaacttcctt ggtttctact ggaatagatt aggtcttttc     2220

caaaaggttg attccgttgc tggtttagct aagttgattg gttgtccaga agctaatgtt     2280

gttgctacat tgaagcaata tgaggagtta tcttccaaaa agcttaatcc ttgtccattg     2340

actggcaagt ctgtctttcc ttgtgtttta ggcactcaag gtccatacta tgttgccttg     2400

gttaccccat ccattcacta cactatgggt ggttgtttga tttccccatc tgctgagatg     2460

caaaccattg acaactctgg tgttactcct gtcagacgtc caatcttagg cttattcggt     2520

gctggtgaag ttactggcgg tgtccatggt ggtaacagat taggcggtaa ctctttgtta     2580

gaatgtgttg ttttcggcaa gatcgctggt gacagagctg caaccatctt gcaaaagaaa     2640

aacaccggct tatcaatgac agaatggtct actgtcgtct taagagaagt tagagaaggt     2700

ggtgtctatg gtgctggttc cagagttttg aggtttaaca tgcctggtgc attacagaga     2760

actggtttag ctttaggtca attcatcggt atcagaggtg attgggacgg tcacagattg     2820

atcggttact attctccaat cactttacct gatgatgttg gtgttattgg tatcttagct     2880

agagcagaca agggtagatt ggcagaatgg atttctgcat tgcagccagg tgacgctgtt     2940

gagatgaagg cctgcggtgg tcttatcatt gacagaagat tcgctgaaag acatttcttt     3000

ttccgtggtc ataagatcag aaagttggcc cttatcggtg gtggtactgg tgttgcacca     3060

atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg tcgattcaat tgagtccatt     3120

cagttcatct atgctgcaga ggatgtttcc gagcttacat acagaacctt acttgaatct     3180

tacgaagagg aatatggttc agaaaagttt aagtgtcact tcgttttgaa taacccacca     3240

gctcaatgga ctgacggtgt tggtttcgtt gatactgcat tgttgagatc cgcagttcaa     3300

gcaccatcaa atgatttgct tgttgcaatt tgtggtccac caatcatgca aagagcagtt     3360

aagggtgcat tgaaaggttt aggttacaat atgaatcttg ttagaaccgt tgacgaaact     3420

gaaccaccat cataa                                                      3435


<210>  33
<211>  3444
<212>  DNA
<213>  Artificial

<220>
<223>  Mutated L. mexicana FRD gene

<400>  33
atggctgatg gcaaaacctc tgcatcagtt gttgctgttg atgctgaacg tgccgctaag       60

gaaagagatg cagcagctag agctatgttg caaggtggtg gtgtctctcc tgctggcaag      120

gcacaattgt tgaaaaaggg tttggttcac actgttccat ataccttaaa ggttgtcgtc      180

gcagatccaa aggaaatgga gaaggcaact gctgacgcag aagaggtttt acaagctgca      240

tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa actcagaagt ttcaagagtc      300

aataggttgg cagttggtga ggaacatcaa atgtctgaaa cattgaaaca cgtcatggcc      360

tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg acccagcagt tggtccatta      420

gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg ttccagccga aagagttaat      480

gatttgttat ccaaatgtac ccttaatgca tctttttcaa ttgatatgtc cagaggtatg      540

attgcaagga agcatccaga cgccatgttg gatttgggtg gtgtcaacaa gggttatggt      600

atcgactaca ttgttgaaca cttaaactct ttgggttatg atgatgtctt tttcgaatgg      660

ggtggtgatg ttagagcatc cggcaaaaac cagttatctc aaccttgggc tgttggtatt      720

gttagaccac ctgccttggc cgacattaga actgttgtcc cagaggacaa aagatccttt      780

atccgtgtcg tcagattgaa caacgaagct attgctacct ctggtgatta tgagaatttg      840

gttgaaggtc ctggttctaa ggtttactct tccaccttca atccaacttc caaaaacttg      900

ttggaaccta ccgaagcagg tatggctcaa gtttctgtca agtgttgctc atgtatctac      960

gctgatgctt tagcaacagc agctttgttg aaaaacgatc ctgctgccgt tagaaggatc     1020

ttagataact ggagatatgt cagagatact gttactgact acaccactta cacaagggaa     1080

ggtgaaagag ttgctaagat gttggaaatt gctaccgaag atgctgaaat gagagcaaag     1140

agaatcaagg gctctttacc agcaagagtt atcattgttg gtggtggttt ggccggttgt     1200

tccgcagcta tcgaagcagc taactgtggc gcccacgtca tcttgttagg taaggaacca     1260

aagttaggtg gtaactctgc aaaggctacc tccggtatca acgcctgggg tactagagca     1320

caagcaaaac aaggtgtcat ggacggcggc aagtttttcg aaagagatac ccatagatcc     1380

ggcaagggtg gtaattgcga tccatgcctt gttaagactt tgtccgttaa gtcctctgat     1440

gcagttaagt ggttatctga attaggtgtt ccattgactg ttttgtctca attaggtggt     1500

gcttcaagga aacgttgtca ccgtgcacca gataagtctg atggtacacc agtcccagtt     1560

ggtttcacca ttatgaaaac ccttgaaaac cacattgtca acgatttgtc cagacatgtt     1620

acagttatga caggtattac cgtcacagct ttagaatcta catcaagagt cagacctgat     1680

ggtgttttag tcaagcatgt tactggtgtt cacttgattc aggcatctgg tcaatctatg     1740

gttttgaatg cagacgctgt tatcttagct actggtggtt tctccaatga tcatacccca     1800

aactcccttt tacaacaata cgccccacag ttgtcatctt ttccaacaac caatggtgtc     1860

tgggcaactg gcgatggtgt taagatggct tccaagttgg gtgtcgcctt agttgatatg     1920

gataaggtcc aattacatcc taccggcttg ttagacccaa aagatccatc taatagaacc     1980

aagtatcttg gtccagaggc cttaagaggt tccggcggtg tcttgttaaa caaaaacggt     2040

gaaagatttg ttaatgaatt agacttaaga tctgttgtct ctcaagctat catcgcacaa     2100

gataatgagt acccaggctc tggtggttcc aagttcgcat actgtgtttt gaacgaaact     2160

gcagcaaagt tattcggcaa aaacttcctt ggtttctact ggaatagatt aggtcttttc     2220

caaaaggttg attccgttgc tggtttagct aagttgattg gttgtccaga agctaatgtt     2280

gttgctacat tgaagcaata tgaggagtta tcttccaaaa agcttaatcc ttgtccattg     2340

actggcaagt ctgtctttcc ttgtgtttta ggcactcaag gtccatacta tgttgccttg     2400

gttaccccat ccattcacta cactatgggt ggttgtttga tttccccatc tgctgagatg     2460

caaaccattg acaactctgg tgttactcct gtcagacgtc caatcttagg cttattcggt     2520

gctggtgaag ttactggcgg tgtccatggt ggtaacagat taggcggtaa ctctttgtta     2580

gaatgtgttg ttttcggcaa gatcgctggt gacagagctg caaccatctt gcaaaagaaa     2640

aacaccggct tatcaatgac agaatggtct actgtcgtct taagagaagt tagagaaggt     2700

ggtgtctatg gtgctggttc cagagttttg aggtttaaca tgcctggtgc attacagaga     2760

actggtttag ctttaggtca attcatcggt atcagaggtg attgggacgg tcacagattg     2820

atcggttact attctccaat cactttacct gatgatgttg gtgttattgg tatcttagct     2880

agagcagaca agggtagatt ggcagaatgg atttctgcat tgcagccagg tgacgctgtt     2940

gagatgaagg cctgcggtgg tcttatcatt gacagaagat tcgctgaaag acatttcttt     3000

ttccgtggtc ataagatcag aaagttggcc cttatcggtg gtggtactgg tgttgcacca     3060

atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg tcgattcaat tgagtccatt     3120

cagttcatct atgctgcaga ggatgtttcc gagcttacat acagaacctt acttgaatct     3180

tacgaagagg aatatggttc agaaaagttt aagtgtcact tcgttttgaa taacccacca     3240

gctcaatgga ctgacggtgt tggtttcgtt gatactgcat tgttgagatc cgcagttcaa     3300

gcaccatcaa atgatttgct tgttgcaatt tgtggtccac caatcatgca aagagcagtt     3360

aagggtgcat tgaaaggttt aggttacaat atgaatcttg ttagaaccgt tgacgaaact     3420

gaaccaccat cataattaat taac                                            3444


<210>  34
<211>  3444
<212>  DNA
<213>  Artificial

<220>
<223>  Mutated L. mexicana FRD gene

<400>  34
atggctgatg gcaaaacctc tgcatcagtt gttgctgttg atgctgaacg tgccgctaag       60

gaaagagatg cagcagctag agctatgttg caaggtggtg gtgtctctcc tgctggcaag      120

gcacaattgt tgaaaaaggg tttggttcac actgttccat ataccttaaa ggttgtcgtc      180

gcagatccaa aggaaatgga gaaggcaact gctgacgcag aagaggtttt acaagctgca      240

tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa actcagaagt ttcaagagtc      300

aataggttgg cagttggtga ggaacatcaa atgtctgaaa cattgaaaca cgtcatggcc      360

tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg acccagcagt tggtccatta      420

gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg ttccagccga aagagttaat      480

gatttgttat ccaaatgtac ccttaatgca tctttttcaa ttgatatgtc cagaggtatg      540

attgcaagga agcatccaga cgccatgttg gatttgggtg gtgtcaacaa gggttatggt      600

atcgactaca ttgttgaaca cttaaactct ttgggttatg atgatgtctt tttcgaatgg      660

ggtggtgatg ttagagcatc cggcaaaaac cagttatctc aaccttgggc tgttggtatt      720

gttagaccac ctgccttggc cgacattaga actgttgtcc cagaggacaa aagatccttt      780

atccgtgtcg tcagattgaa caacgaagct attgctacct ctggtgatta tgagaatttg      840

gttgaaggtc ctggttctaa ggtttactct tccaccttca atccaacttc caaaaacttg      900

ttggaaccta ccgaagcagg tatggctcaa gtttctgtca agtgttgctc atgtatctac      960

gctgatgctt tagcaacagc agctttgttg aaaaacgatc ctgctgccgt tagaaggatc     1020

ttagataact ggagatatgt cagagatact gttactgact acaccactta cacaagggaa     1080

ggtgaaagag ttgctaagat gttggaaatt gctaccgaag atgctgaaat gagagcaaag     1140

agaatcaagg gctctttacc agcaagagtt atcattgttg gtggtggttt ggccggttgt     1200

tccgcagcta tcgaagcagc taactgtggc gcccacgtca tcttgttaga aaaggaacca     1260

aagttaggtg gtaactctgc aaaggctacc tccggtatca acgcctgggg tactagagca     1320

caagcaaaac aaggtgtcat ggacggcggc aagtttttcg aaagagatac ccatagatcc     1380

ggcaagggtg gtaattgcga tccatgcctt gttaagactt tgtccgttaa gtcctctgat     1440

gcagttaagt ggttatctga attaggtgtt ccattgactg ttttgtctca attaggtggt     1500

gcttcaagga aacgttgtca ccgtgcacca gataagtctg atggtacacc agtcccagtt     1560

ggtttcacca ttatgaaaac ccttgaaaac cacattgtca acgatttgtc cagacatgtt     1620

acagttatga caggtattac cgtcacagct ttagaatcta catcaagagt cagacctgat     1680

ggtgttttag tcaagcatgt tactggtgtt cacttgattc aggcatctgg tcaatctatg     1740

gttttgaatg cagacgctgt tatcttagct actggtggtt tctccaatga tcatacccca     1800

aactcccttt tacaacaata cgccccacag ttgtcatctt ttccaacaac caatggtgtc     1860

tgggcaactg gcgatggtgt taagatggct tccaagttgg gtgtcgcctt agttgatatg     1920

ggtaaggtcc aattacatcc taccggcttg ttagacccaa aagatccatc taatagaacc     1980

aagtatcttg gtccagaggc cttaagaggt tccggcggtg tcttgttaaa caaaaacggt     2040

gaaagatttg ttaatgaatt agacttaaga tctgttgtct ctcaagctat catcgcacaa     2100

gataatgagt acccaggctc tggtggttcc aagttcgcat actgtgtttt gaacgaaact     2160

gcagcaaagt tattcggcaa aaacttcctt ggtttctact ggaatagatt aggtcttttc     2220

caaaaggttg attccgttgc tggtttagct aagttgattg gttgtccaga agctaatgtt     2280

gttgctacat tgaagcaata tgaggagtta tcttccaaaa agcttaatcc ttgtccattg     2340

actggcaagt ctgtctttcc ttgtgtttta ggcactcaag gtccatacta tgttgccttg     2400

gttaccccat ccattcacta cactatgggt ggttgtttga tttccccatc tgctgagatg     2460

caaaccattg acaactctgg tgttactcct gtcagacgtc caatcttagg cttattcggt     2520

gctggtgaag ttactggcgg tgtccatggt ggtaacagat taggcggtaa ctctttgtta     2580

gaatgtgttg ttttcggcaa gatcgctggt gacagagctg caaccatctt gcaaaagaaa     2640

aacaccggct tatcaatgac agaatggtct actgtcgtct taagagaagt tagagaaggt     2700

ggtgtctatg gtgctggttc cagagttttg aggtttaaca tgcctggtgc attacagaga     2760

actggtttag ctttaggtca attcatcggt atcagaggtg attgggacgg tcacagattg     2820

atcggttact attctccaat cactttacct gatgatgttg gtgttattgg tatcttagct     2880

agagcagaca agggtagatt ggcagaatgg atttctgcat tgcagccagg tgacgctgtt     2940

gagatgaagg cctgcggtgg tcttatcatt gacagaagat tcgctgaaag acatttcttt     3000

ttccgtggtc ataagatcag aaagttggcc cttatcggtg gtggtactgg tgttgcacca     3060

atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg tcgattcaat tgagtccatt     3120

cagttcatct atgctgcaga ggatgtttcc gagcttacat acagaacctt acttgaatct     3180

tacgaagagg aatatggttc agaaaagttt aagtgtcact tcgttttgaa taacccacca     3240

gctcaatgga ctgacggtgt tggtttcgtt gatactgcat tgttgagatc cgcagttcaa     3300

gcaccatcaa atgatttgct tgttgcaatt tgtggtccac caatcatgca aagagcagtt     3360

aagggtgcat tgaaaggttt aggttacaat atgaatcttg ttagaaccgt tgacgaaact     3420

gaaccaccat cataattaat taac                                            3444


<210>  35
<211>  3435
<212>  DNA
<213>  Artificial

<220>
<223>  Mutated L. mexicana FRD gene

<400>  35
atggctgatg gcaaaacctc tgcatcagtt gttgctgttg atgctgaacg tgccgctaag       60

gaaagagatg cagcagctag agctatgttg caaggtggtg gtgtctctcc tgctggcaag      120

gcacaattgt tgaaaaaggg tttggttcac actgttccat ataccttaaa ggttgtcgtc      180

gcagatccaa aggaaatgga gaaggcaact gctgacgcag aagaggtttt acaagctgca      240

tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa actcagaagt ttcaagagtc      300

aataggttgg cagttggtga ggaacatcaa atgtctgaaa cattgaaaca cgtcatggcc      360

tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg acccagcagt tggtccatta      420

gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg ttccagccga aagagttaat      480

gatttgttat ccaaatgtac ccttaatgca tctttttcaa ttgatatgtc cagaggtatg      540

attgcaagga agcatccaga cgccatgttg gatttgggtg gtgtcaacaa gggttatggt      600

atcgactaca ttgttgaaca cttaaactct ttgggttatg atgatgtctt tttcgaatgg      660

ggtggtgatg ttagagcatc cggcaaaaac cagttatctc aaccttgggc tgttggtatt      720

gttagaccac ctgccttggc cgacattaga actgttgtcc cagaggacaa aagatccttt      780

atccgtgtcg tcagattgaa caacgaagct attgctacct ctggtgatta tgagaatttg      840

gttgaaggtc ctggttctaa ggtttactct tccaccttca atccaacttc caaaaacttg      900

ttggaaccta ccgaagcagg tatggctcaa gtttctgtca agtgttgctc atgtatctac      960

gctgatgctt tagcaacagc agctttgttg aaaaacgatc ctgctgccgt tagaaggatc     1020

ttagataact ggagatatgt cagagatact gttactgact acaccactta cacaagggaa     1080

ggtgaaagag ttgctaagat gttggaaatt gctaccgaag atgctgaaat gagagcaaag     1140

agaatcaagg gctctttacc agcaagagtt atcattgttg gtggtggttt ggccggttgt     1200

tccgcagcta tcgaagcagc taactgtggc gcccacgtca tcttgttaga aaaggaacca     1260

aagttaggtg gtaactctgc aaaggctacc tccggtatca acgcctgggg tactagagca     1320

caagcaaaac aaggtgtcat ggacggcggc aagtttttcg aaagagatac ccatagatcc     1380

ggcaagggtg gtaattgcga tccatgcctt gttaagactt tgtccgttaa gtcctctgat     1440

gcagttaagt ggttatctga attaggtgtt ccattgactg ttttgtctca attaggtggt     1500

gcttcaagga aacgttgtca ccgtgcacca gataagtctg atggtacacc agtcccagtt     1560

ggtttcacca ttatgaaaac ccttgaaaac cacattgtca acgatttgtc cagacatgtt     1620

acagttatga caggtattac cgtcacagct ttagaatcta catcaagagt cagacctgat     1680

ggtgttttag tcaagcatgt tactggtgtt cacttgattc aggcatctgg tcaatctatg     1740

gttttgaatg cagacgctgt tatcttagct actggtggtt tctccaatga tcatacccca     1800

aactcccttt tacaacaata cgccccacag ttgtcatctt ttccaacaac caatggtgtc     1860

tgggcaactg gcgatggtgt taagatggct tccaagttgg gtgtcgcctt agttgatatg     1920

gataaggtcc aattacatcc taccggcttg ttagacccaa aagatccatc taatagaacc     1980

aagtatcttg gtccagaggc cttaagaggt tccggcggtg tcttgttaaa caaaaacggt     2040

gaaagatttg ttaatgaatt agacttaaga tctgttgtct ctcaagctat catcgcacaa     2100

gataatgagt acccaggctc tggtggttcc aagttcgcat actgtgtttt gaacgaaact     2160

gcagcaaagt tattcggcaa aaacttcctt ggtttctact ggaatagatt aggtcttttc     2220

caaaaggttg attccgttgc tggtttagct aagttgattg gttgtccaga agctaatgtt     2280

gttgctacat tgaagcaata tgaggagtta tcttccaaaa agcttaatcc ttgtccattg     2340

actggcaagt ctgtctttcc ttgtgtttta ggcactcaag gtccatacta tgttgccttg     2400

gttaccccat ccattcacta cactatgggt ggttgtttga tttccccatc tgctgagatg     2460

caaaccattg acaactctgg tgttactcct gtcagacgtc caatcttagg cttattcggt     2520

gctggtgaag ttactggcgg tgtccatggt ggtaacagat taggcggtaa ctctttgtta     2580

ggacgtgttg ttttcggcaa gatcgctggt gacagagctg caaccatctt gcaaaagaaa     2640

aacaccggct tatcaatgac agaatggtct actgtcgtct taagagaagt tagagaaggt     2700

ggtgtctatg gtgctggttc cagagttttg aggtttaaca tgcctggtgc attacagaga     2760

actggtttag ctttaggtca attcatcggt atcagaggtg attgggacgg tcacagattg     2820

atcggttact attctccaat cactttacct gatgatgttg gtgttattgg tatcttagct     2880

agagcagaca agggtagatt ggcagaatgg atttctgcat tgcagccagg tgacgctgtt     2940

gagatgaagg cctgcggtgg tcttatcatt gacagaagat tcgctgaaag acatttcttt     3000

ttccgtggtc ataagatcag aaagttggcc cttatcggtg gtggtactgg tgttgcacca     3060

atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg tcgattcaat tgagtccatt     3120

cagttcatct atgctgcaga ggatgtttcc gagcttacat acagaacctt acttgaatct     3180

tacgaagagg aatatggttc agaaaagttt aagtgtcact tcgttttgaa taacccacca     3240

gctcaatgga ctgacggtgt tggtttcgtt gatactgcat tgttgagatc cgcagttcaa     3300

gcaccatcaa atgatttgct tgttgcaatt tgtggtccac caatcatgca aagagcagtt     3360

aagggtgcat tgaaaggttt aggttacaat atgaatcttg ttagaaccgt tgacgaaact     3420

gaaccaccat cataa                                                      3435


<210>  36
<211>  3435
<212>  DNA
<213>  Artificial

<220>
<223>  Mutated L. mexicana FRD gene

<400>  36
atggctgatg gcaaaacctc tgcatcagtt gttgctgttg atgctgaacg tgccgctaag       60

gaaagagatg cagcagctag agctatgttg caaggtggtg gtgtctctcc tgctggcaag      120

gcacaattgt tgaaaaaggg tttggttcac actgttccat ataccttaaa ggttgtcgtc      180

gcagatccaa aggaaatgga gaaggcaact gctgacgcag aagaggtttt acaagctgca      240

tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa actcagaagt ttcaagagtc      300

aataggttgg cagttggtga ggaacatcaa atgtctgaaa cattgaaaca cgtcatggcc      360

tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg acccagcagt tggtccatta      420

gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg ttccagccga aagagttaat      480

gatttgttat ccaaatgtac ccttaatgca tctttttcaa ttgatatgtc cagaggtatg      540

attgcaagga agcatccaga cgccatgttg gatttgggtg gtgtcaacaa gggttatggt      600

atcgactaca ttgttgaaca cttaaactct ttgggttatg atgatgtctt tttcgaatgg      660

ggtggtgatg ttagagcatc cggcaaaaac cagttatctc aaccttgggc tgttggtatt      720

gttagaccac ctgccttggc cgacattaga actgttgtcc cagaggacaa aagatccttt      780

atccgtgtcg tcagattgaa caacgaagct attgctacct ctggtgatta tgagaatttg      840

gttgaaggtc ctggttctaa ggtttactct tccaccttca atccaacttc caaaaacttg      900

ttggaaccta ccgaagcagg tatggctcaa gtttctgtca agtgttgctc atgtatctac      960

gctgatgctt tagcaacagc agctttgttg aaaaacgatc ctgctgccgt tagaaggatc     1020

ttagataact ggagatatgt cagagatact gttactgact acaccactta cacaagggaa     1080

ggtgaaagag ttgctaagat gttggaaatt gctaccgaag atgctgaaat gagagcaaag     1140

agaatcaagg gctctttacc agcaagagtt atcattgttg gtggtggttt ggccggttgt     1200

tccgcagcta tcgaagcagc taactgtggc gcccacgtca tcttgttaga aaaggaacca     1260

aagttaggtg gtaactctgc aaaggctacc tccggtatca acgcctgggg tactagagca     1320

caagcaaaac aaggtgtcat ggacggcggc aagtttttcg aaagagatac ccatagatcc     1380

ggcaagggtg gtaattgcga tccatgcctt gttaagactt tgtccgttaa gtcctctgat     1440

gcagttaagt ggttatctga attaggtgtt ccattgactg ttttgtctca attaggtggt     1500

gcttcaagga aacgttgtca ccgtgcacca gataagtctg atggtacacc agtcccagtt     1560

ggtttcacca ttatgaaaac ccttgaaaac cacattgtca acgatttgtc cagacatgtt     1620

acagttatga caggtattac cgtcacagct ttagaatcta catcaagagt cagacctgat     1680

ggtgttttag tcaagcatgt tactggtgtt cacttgattc aggcatctgg tcaatctatg     1740

gttttgaatg cagacgctgt tatcttagct actggtggtt tctccaatga tcatacccca     1800

aactcccttt tacaacaata cgccccacag ttgtcatctt ttccaacaac caatggtgtc     1860

tgggcaactg gcgatggtgt taagatggct tccaagttgg gtgtcgcctt agttgatatg     1920

gataaggtcc aattacatcc taccggcttg ttagacccaa aagatccatc taatagaacc     1980

aagtatcttg gtccagaggc cttaagaggt tccggcggtg tcttgttaaa caaaaacggt     2040

gaaagatttg ttaatgaatt agacttaaga tctgttgtct ctcaagctat catcgcacaa     2100

gataatgagt acccaggctc tggtggttcc aagttcgcat actgtgtttt gaacgaaact     2160

gcagcaaagt tattcggcaa aaacttcctt ggtttctact ggaatagatt aggtcttttc     2220

caaaaggttg attccgttgc tggtttagct aagttgattg gttgtccaga agctaatgtt     2280

gttgctacat tgaagcaata tgaggagtta tcttccaaaa agcttaatcc ttgtccattg     2340

actggcaagt ctgtctttcc ttgtgtttta ggcactcaag gtccatacta tgttgccttg     2400

gttaccccat ccattcacta cactatgggt ggttgtttga tttccccatc tgctgagatg     2460

caaaccattg acaactctgg tgttactcct gtcagacgtc caatcttagg cttattcggt     2520

gctggtgaag ttactggcgg tgtccatggt ggtaacagat taggcggtaa ctctttgtta     2580

gaatgtgttg ttttcggcaa gatcgctggt gacagagctg caaccatctt gcaaaagaaa     2640

aacaccggct tatcaatgac agaatggtct actgtcgtct taagagaagt tagagaaggt     2700

ggtgtctatg gtgctggttc cagagttttg aggtttaaca tgcctggtgc attacagaga     2760

actggtttag ctttaggtca attcatcggt atcagaggtg attgggacgg tcacagattg     2820

atcggttact attctccaat cactttacct gatgatgttg gtgttattgg tatcttagct     2880

agagcagaca agggtagatt ggcagaatgg atttctgcat tgcagccagg tgacgctgtt     2940

gagatgaagg cctgcggtgg tcttatcatt gacagaagat tcgctgaaag acatttcttt     3000

ttccgtggtc ataagatcag aaagttggcc cttatcggtg gtggtactgg tgttgcacca     3060

atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg tcggtcgaat tgagtccatt     3120

cagttcatct atgctgcaga ggatgtttcc gagcttacat acagaacctt acttgaatct     3180

tacgaagagg aatatggttc agaaaagttt aagtgtcact tcgttttgaa taacccacca     3240

gctcaatgga ctgacggtgt tggtttcgtt gatactgcat tgttgagatc cgcagttcaa     3300

gcaccatcaa atgatttgct tgttgcaatt tgtggtccac caatcatgca aagagcagtt     3360

aagggtgcat tgaaaggttt aggttacaat atgaatcttg ttagaaccgt tgacgaaact     3420

gaaccaccat cataa                                                      3435


<210>  37
<211>  3420
<212>  DNA
<213>  Artificial

<220>
<223>  Mutated T.brucei FRD gene

<400>  37
atggttgatg gtagatcttc agcttctatt gttgcagttg atccagaaag agcagcaaga       60

gaaagagatg ctgcagctag agctttgtta caagattctc cattgcacac taccatgcaa      120

tatgctacct ccggtttaga attgaccgtc ccttatgcat tgaaagttgt tgcatctgcc      180

gacaccttcg atagagctaa ggaagttgca gatgaagtcc ttagatgtgc ctggcaattg      240

gctgatacag tccttaactc ctttaaccca aactctgaag tctctcttgt tggtagactt      300

ccagtcggtc agaagcatca aatgtccgcc ccacttaaga gagttatggc ttgttgtcaa      360

agagtttaca attcctctgc tggttgtttc gacccatcca ccgccccagt tgcaaaggct      420

ttgcgtgaaa tcgctttagg caaggagaga aacaatgcct gtttggaggc tttaacacaa      480

gcatgcactt tgccaaactc tttcgtcatt gactttgaag caggtactat ctcacgtaaa      540

catgaacatg cttcacttga cttaggtggt gtttcaaagg gttacatcgt tgactatgtt      600

attgataaca ttaacgcagc tggtttccaa aatgtctttt tcgattgggg tggtgattgt      660

agagcctccg gtatgaatgc tagaaatacc ccttgggttg ttggtattac tagaccacca      720

tcattagata tgttaccaaa cccaccaaag gaagcatcct atatctctgt tatctcattg      780

gacaacgaag ctttggcaac ctccggtgat tacgagaatt tgatctacac agctgatgac      840

aagcctttaa cttgtactta cgattggaag ggcaaggaac ttatgaagcc atctcaatca      900

aacattgccc aagtttcagt taagtgctat tcagcaatgt acgctgacgc tttagccacc      960

gcttgtttca tcaaaagaga tccagccaag gttagacaat tgttagatgg ttggagatac     1020

gttagagata ctgtcagaga ttacagagtt tatgttagag aaaatgagag agtcgctaag     1080

atgtttgaaa ttgcaaccga agatgctgaa atgagaaaaa gacgtatctc taatactttg     1140

cctgcaagag tcatcgttgt cggtggcggt ttagcaggtt tatctgcagc aattgaagct     1200

gcaggctgcg gtgcacaagt cgttttgatg ggaaaggaag ctaagttagg tggtaactct     1260

gcaaaggcaa cctctggtat caatggttgg ggtactagag cccaagcaaa ggcttccatt     1320

gttgacggtg gcaagtattt cgaaagagat acttacaaat ctggtattgg tggtaatacc     1380

gacccagctt tagttaagac tctttccatg aagtctgctg acgctattgg ttggttaaca     1440

tcattaggtg ttcctttaac agtcttatca caattgggtg gtcattccag aaagagaact     1500

cacagagcac cagacaaaaa ggatggcacc ccattaccta ttggttttac cattatgaaa     1560

accttagaag atcacgtcag aggtaatctt tctggtagaa ttactatcat ggaaaactgt     1620

tccgttacct ctttactttc tgaaactaag gaaagaccag atggtactaa acaaatcaga     1680

gttaccggtg ttgagttcac tcaagcaggc tctggcaaaa ctaccatttt ggccgacgca     1740

gtcatcttgg ccactggtgg tttctctaac gacaagaccg cagactcttt gttgagagaa     1800

catgcccctc acttagttaa ctttcctaca actaacggtc cttgggcaac tggtgacggt     1860

gttaagcttg ctcaaagatt aggtgcacaa ttggtcgaca tggataaggt tcaattgcat     1920

ccaactggtt tgattaaccc aaaagatcca gctaatccaa caaagttttt gggtccagaa     1980

gctttaagag gttccggtgg tgtcttgtta aacaaacagg gtaaaagatt tgttaacgaa     2040

ttagatttgc gttctgttgt ttccaaggcc attatggaac aaggtgctga atacccaggc     2100

tctggtggtt ctatgttcgc atattgtgtc cttaatgcag ctgcacaaaa gttgtttggt     2160

gtctcttccc acgagttcta ctggaaaaag atgggtttgt tcgttaaggc tgatactatg     2220

agagatttgg cagcattgat tggttgtcca gtcgagtctg ttcaacaaac tttagaggaa     2280

tatgaaagat tatctatttc tcagagatcc tgtccaatca ctagaaaatc tgtttaccca     2340

tgtgttttgg gcactaaggg tccatactac gttgctttcg tcaccccatc tattcactat     2400

acaatgggtg gttgtttgat ttccccatca gcagaaattc agatgaaaaa cacctcctcc     2460

cgtgctccat tgtcccattc caaccctatc ttgggtttgt tcggtgctgg tgaagttact     2520

ggtggtgtcc acggtggcaa tagattaggt ggtaactcat tgttagaatg tgttgtcttt     2580

ggtagaattg ctggtgatag agcttctacc attttgcaga gaaagtcctc cgcattatct     2640

ttcaaggtct ggactaccgt tgttttgaga gaagttagag aaggtggcgt ctatggtgcc     2700

ggttcaagag ttttgagatt caacttgcct ggtgctttac aaagatccgg tttgtccttg     2760

ggtcaattca tcgcaatcag aggtgactgg gatggtcaac aattgattgg ttactattcc     2820

ccaattacat tgccagatga cttgggtatg attgacattt tggctagatc cgataaaggt     2880

actttaagag aatggatttc tgctttagaa ccaggcgacg ctgttgagat gaaagcatgc     2940

ggtggtttag tcatcgagag aagattgtca gataagcact ttgtctttat gggtcacatc     3000

attaacaagt tatgtttgat cgctggtggt acaggcgttg cacctatgtt acaaatcatt     3060

aaggcagcat tcatgaaacc ttttatcgat accttagaat ctgtccatct tatctatgct     3120

gcagaagatg ttaccgagtt aacttataga gaagttttag aggagcgtag aagagagtct     3180

cgtggcaagt tcaaaaagac ctttgttttg aacagacctc caccactttg gactgatggt     3240

gttggtttca tcgatagagg tatcttaact aatcatgtcc aaccaccatc cgataacctt     3300

ttggttgcaa tctgtggtcc acctgtcatg cagcgtattg ttaaggccac cttaaagact     3360

ttgggttaca atatgaatct tgttagaaca gttgacgaaa cagaaccatc cggttcctaa     3420


<210>  38
<211>  3889
<212>  DNA
<213>  Artificial

<220>
<223>  GPD gene deletion fragment

<400>  38
ccttcattta cgaaataaag tgccgcggtt acgcagcaca caccagcaat cacgtgcagt       60

gtctttttct tttttttttc ttttttttcc tctttttctt ttgttttgtt tcgtttcttt      120

tccgccagtt cccgttttcc atttccggaa caacaatggg actccactgt tttctttccc      180

cccttccgtt ttcggctcgc agtctgtaca tgcacgttta tccgacacct gtcttgtttg      240

gcgcgtaatt aatacagttt ctccggagtc caggtctcgg acgggtaatt tacacgtcat      300

cattcatttc tgtgtcaaga gaggtagcgc aaaaagtaga aatggtgaac cacgggaatg      360

acttgctgga aatcgacgcc agagtccatt tgaaaaccta cctctacaag agaggaaaca      420

cactacaggg tgtccctggt ccgtaaaatg gcgtaatatg atgacttccc tctatagacg      480

ttgtatttcc agctccaaca tggttaaact attgctatgg tgatggtatt acagatagta      540

aaagaaggaa gggggggtgg caatctcacc ctaacagtta ctaagaacgt ctacttcatc      600

tactgtcaat atacattggc cacatgccga gaaattacgt cgacgccaaa gaagggctca      660

gccgaaaaaa gaaatggaaa acttggccga aaagggaaac aaacaaaaag gtgatgtaaa      720

attagcggaa aggggaattg gcaaattgag ggagaaaaaa aaaaggcaga aaaggaggcg      780

gaaagtcagt acgttttgaa ggcgtcattg gttttccctt ttgcagagtg tttcatttct      840

tttgtttcat gacgtagtgg cgtttctttt cctgcacttt agaaatctat cttttcctta      900

tcaagtaaca agcggttggc aaaggtgtat ataaatcaag gaattcccac tttgaaccct      960

ttgaattttg atatcgttta ttttaaattt atttgcggcc gcggatccct cgaggcctta     1020

attaacatct gaatgtaaaa tgaacattaa aatgaattac taaactttac gtctacttta     1080

caatctataa actttgttta atcatataac gaaatacact aatacacaat cctgtacgta     1140

tgtaatactt ttatccatca aggattgaga aaaaaaagta atgattccct gggccattaa     1200

aacttagacc cccaagcttg gataggtcac tctctatttt cgtttctccc ttccctgata     1260

gaagggtgat atgtaattaa gaataatata taattttata ataaaagaat tcatagcctc     1320

atgaaatcag ccatttgctt ttgttcaacg atcttttgaa attgttgttg ttcttggtag     1380

ttaagttgat ccatcttggc ttatgttgtg tgtatgttgt agttattctt agtatattcc     1440

tgtcctgagt ttagtgaaac ataatatcgc cttgaaatga aaatgctgaa attcgtcgac     1500

atacaatttt tcaaactttt tttttttctt ggtgcacgga catgttttta aaggaagtac     1560

tctataccag ttattcttca caaatttaat tgctggagaa tagatcttca acgctttaat     1620

aaagtagttt gtttgtcaag gatggcgtca tacaaagaaa gatcagaatc acacacttcc     1680

cctgttgcta ggagactttt ctccatcatg gaggaaaaga agtctaacct ttgtgcatca     1740

ttggatatta ctgaaactga aaagcttctc tctattttgg acactattgg tccttacatc     1800

tgtctagtta aaacacacat cgatattgtt tctgatttta cgtatgaagg aactgtgttg     1860

cctttgaagg agcttgccaa gaaacataat tttatgattt ttgaagatag aaaatttgct     1920

gatattggta acactgttaa aaatcaatat aaatctggtg tcttccgtat tgccgaatgg     1980

gctgacatca ctaatgcaca tggtgtaacg ggtgcaggta ttgtttctgg cttgaaggag     2040

gcagcccaag aaacaaccag tgaacctaga ggtttgctaa tgcttgctga gttatcatca     2100

aagggttctt tagcatatgg tgaatataca gaaaaaacag tagaaattgc taaatctgat     2160

aaagagtttg tcattggttt tattgcgcaa cacgatatgg gcggtagaga agaaggtttt     2220

gactggatca ttatgactcc aggggttggt ttagatgaca aaggtgatgc acttggtcaa     2280

caatatagaa ctgttgatga agttgtaaag actggaacgg atatcataat tgttggtaga     2340

ggtttgtacg gtcaaggaag agatcctata gagcaagcta aaagatacca acaagctggt     2400

tggaatgctt atttaaacag atttaaatga ttcttacaca aagatttgat acatgtacac     2460

tagtttaaat aagcatgaaa agaattacac aagcaaaaaa aaaaaaataa atgaggtact     2520

ttacgttcac ctacaaccaa aaaaactaga tagagtaaaa tcttaagatt tagaaaaagt     2580

tgtttaacaa aggctttagt atgtgaattt ttaatgtagc aaagcgataa ctaataaaca     2640

taaacaaaag tatggttttc tttatcagtc aaatcattat cgattgattg ttccgcgtat     2700

ctgcagatag cctcatgaaa tcagccattt gcttttgttc aacgatcttt tgaaattgtt     2760

gttgttcttg gtagttaagt tgatccatct tggcttatgt tgtgtgtatg ttgtagttat     2820

tcttagtata ttcctgtcct gagtttagtg aaacataata tcgccttgaa atgaaaatgc     2880

tgaaattcgt cgacatacaa tttttcaaac tttttttttt tcttggtgca cggacatgtt     2940

tttaaaggaa gtactctata ccagttattc ttcacaaatt taattgctgg agaatagatc     3000

ttcaacgccc cgggggatct ggatccgcgg ccgcaataac ctcagggaga actttggcat     3060

tgtactctcc attgacgagt ccgccaaccc attcttgtta aacctaacct tgcattatca     3120

cattcccttt gacccccttt agctgcattt ccacttgtct acattaagat tcattacaca     3180

ttctttttcg tatttctctt acctccctcc cccctccatg gatcttatat ataaatcttt     3240

tctataacaa taatatctac tagagttaaa caacaattcc acttggcatg gctgtctcag     3300

caaatctgct tctacctact gcacgggttt gcatgtcatt gtttctagca gggaatcgtc     3360

catgtacgtt gtcctccatg atggtcttcc cgctgccact ttctttagta tcttaaatag     3420

agcagatctt acgtccactg tgcatccgtg caccccgaaa atcgtatggt tttccttgcc     3480

acctctcaca attttgaata tgctcaacgc gaaagagagg ggaagaggaa tcgcattcgt     3540

agagtggcta cattcaaccc tgacaaagga actagcgttt gtgcaggaga gagtggtttg     3600

catagatttc ctttcctttg caagcatatt atatagagta gccaatacag taacagctac     3660

agcacaaaaa agagaacgag aacgagaacg agaacaagaa caagaactag cactactgtc     3720

actgccagca tcaacattac taccattatt ccaacatgtt tgcaactaga aatataacca     3780

ttggtgtcag aacactcaga ccaaccagtt tcttgaaaac aaggtctttt ctgcaacaga     3840

ggctacaatc aacgctaaag aagagctatg aaccaaccaa atccgagct                 3889


<210>  39
<211>  3889
<212>  DNA
<213>  Artificial

<220>
<223>  GPD gene deletion fragment

<400>  39
ccttcattta cgaaataaag tgccgcggtt acgcagcaca caccagcaat cacgtgcagt       60

gtctttttct tttttttttc ttttttttcc tctttttctt ttgttttgtt tcgtttcttt      120

tccgccagtt cccgttttcc atttccggaa caacaatggg actccactgt tttctttccc      180

cccttccgtt ttcggctcgc agtctgtaca tgcacgttta tccgacacct gtcttgtttg      240

gcgcgtaatt aatacagttt ctccggagtc caggtctcgg acgggtaatt tacacgtcat      300

cattcatttc tgtgtcaaga gaggtagcgc aaaaagtaga aatggtgaac cacgggaatg      360

acttgctgga aatcgacgcc agagtccatt tgaaaaccta cctctacaag agaggaaaca      420

cactacaggg tgtccctggt ccgtaaaatg gcgtaatatg atgacttccc tctatagacg      480

ttgtatttcc agctccaaca tggttaaact attgctatgg tgatggtatt acagatagta      540

aaagaaggaa gggggggtgg caatctcacc ctaacagtta ctaagaacgt ctacttcatc      600

tactgtcaat atacattggc cacatgccga gaaattacgt cgacgccaaa gaagggctca      660

gccgaaaaaa gaaatggaaa acttggccga aaagggaaac aaacaaaaag gtgatgtaaa      720

attagcggaa aggggaattg gcaaattgag ggagaaaaaa aaaaggcaga aaaggaggcg      780

gaaagtcagt acgttttgaa ggcgtcattg gttttccctt ttgcagagtg tttcatttct      840

tttgtttcat gacgtagtgg cgtttctttt cctgcacttt agaaatctat cttttcctta      900

tcaagtaaca agcggttggc aaaggtgtat ataaatcaag gaattcccac tttgaaccct      960

ttgaattttg atatcgttta ttttaaattt atttgcggcc gcggatccag atcccccggg     1020

gcgttgaaga tctattctcc agcaattaaa tttgtgaaga ataactggta tagagtactt     1080

cctttaaaaa catgtccgtg caccaagaaa aaaaaaaagt ttgaaaaatt gtatgtcgac     1140

gaatttcagc attttcattt caaggcgata ttatgtttca ctaaactcag gacaggaata     1200

tactaagaat aactacaaca tacacacaac ataagccaag atggatcaac ttaactacca     1260

agaacaacaa caatttcaaa agatcgttga acaaaagcaa atggctgatt tcatgaggct     1320

atctgcagat acgcggaaca atcaatcgat aatgatttga ctgataaaga aaaccatact     1380

tttgtttatg tttattagtt atcgctttgc tacattaaaa attcacatac taaagccttt     1440

gttaaacaac tttttctaaa tcttaagatt ttactctatc tagttttttt ggttgtaggt     1500

gaacgtaaag tacctcattt attttttttt ttttgcttgt gtaattcttt tcatgcttat     1560

ttaaactagt gtacatgtat caaatctttg tgtaagaatc atttaaatct gtttaaataa     1620

gcattccaac cagcttgttg gtatctttta gcttgctcta taggatctct tccttgaccg     1680

tacaaacctc taccaacaat tatgatatcc gttccagtct ttacaacttc atcaacagtt     1740

ctatattgtt gaccaagtgc atcacctttg tcatctaaac caacccctgg agtcataatg     1800

atccagtcaa aaccttcttc tctaccgccc atatcgtgtt gcgcaataaa accaatgaca     1860

aactctttat cagatttagc aatttctact gttttttctg tatattcacc atatgctaaa     1920

gaaccctttg atgataactc agcaagcatt agcaaacctc taggttcact ggttgtttct     1980

tgggctgcct ccttcaagcc agaaacaata cctgcacccg ttacaccatg tgcattagtg     2040

atgtcagccc attcggcaat acggaagaca ccagatttat attgattttt aacagtgtta     2100

ccaatatcag caaattttct atcttcaaaa atcataaaat tatgtttctt ggcaagctcc     2160

ttcaaaggca acacagttcc ttcatacgta aaatcagaaa caatatcgat gtgtgtttta     2220

actagacaga tgtaaggacc aatagtgtcc aaaatagaga gaagcttttc agtttcagta     2280

atatccaatg atgcacaaag gttagacttc ttttcctcca tgatggagaa aagtctccta     2340

gcaacagggg aagtgtgtga ttctgatctt tctttgtatg acgccatcct tgacaaacaa     2400

actactttat taaagcgttg aagatctatt ctccagcaat taaatttgtg aagaataact     2460

ggtatagagt acttccttta aaaacatgtc cgtgcaccaa gaaaaaaaaa aagtttgaaa     2520

aattgtatgt cgacgaattt cagcattttc atttcaaggc gatattatgt ttcactaaac     2580

tcaggacagg aatatactaa gaataactac aacatacaca caacataagc caagatggat     2640

caacttaact accaagaaca acaacaattt caaaagatcg ttgaacaaaa gcaaatggct     2700

gatttcatga ggctatgaat tcttttatta taaaattata tattattctt aattacatat     2760

cacccttcta tcagggaagg gagaaacgaa aatagagagt gacctatcca agcttggggg     2820

tctaagtttt aatggcccag ggaatcatta cttttttttc tcaatccttg atggataaaa     2880

gtattacata cgtacaggat tgtgtattag tgtatttcgt tatatgatta aacaaagttt     2940

atagattgta aagtagacgt aaagtttagt aattcatttt aatgttcatt ttacattcag     3000

atgttaatta aggcctcgag ggatccgcgg ccgcaataac ctcagggaga actttggcat     3060

tgtactctcc attgacgagt ccgccaaccc attcttgtta aacctaacct tgcattatca     3120

cattcccttt gacccccttt agctgcattt ccacttgtct acattaagat tcattacaca     3180

ttctttttcg tatttctctt acctccctcc cccctccatg gatcttatat ataaatcttt     3240

tctataacaa taatatctac tagagttaaa caacaattcc acttggcatg gctgtctcag     3300

caaatctgct tctacctact gcacgggttt gcatgtcatt gtttctagca gggaatcgtc     3360

catgtacgtt gtcctccatg atggtcttcc cgctgccact ttctttagta tcttaaatag     3420

agcagatctt acgtccactg tgcatccgtg caccccgaaa atcgtatggt tttccttgcc     3480

acctctcaca attttgaata tgctcaacgc gaaagagagg ggaagaggaa tcgcattcgt     3540

agagtggcta cattcaaccc tgacaaagga actagcgttt gtgcaggaga gagtggtttg     3600

catagatttc ctttcctttg caagcatatt atatagagta gccaatacag taacagctac     3660

agcacaaaaa agagaacgag aacgagaacg agaacaagaa caagaactag cactactgtc     3720

actgccagca tcaacattac taccattatt ccaacatgtt tgcaactaga aatataacca     3780

ttggtgtcag aacactcaga ccaaccagtt tcttgaaaac aaggtctttt ctgcaacaga     3840

ggctacaatc aacgctaaag aagagctatg aaccaaccaa atccgagct                 3889


<210>  40
<211>  3175
<212>  DNA
<213>  Artificial

<220>
<223>  PGI gene deletion construct

<400>  40
cttcgctcgc catctatatc ttcaacgaac aacggaatta caaacatggg cagtagttca       60

aacaatctcc agactctcaa ctctctctcg ctatcgttga aacatccaca gttccaaggc      120

ctattctccc cactggatgt ccacagtccg tacgaacaga acgtttcttc cccactggcc      180

cccaccgttc cggctgttcc gggaaccgca ccttcattcg agtcggacga tctctacaat      240

gcaacggctg cccgcaaaag agactctctc aagatgaaga gaagatagac gctacatcat      300

tgtctgtgca gtacctaata tatagtactt ggtataaggt ataataaagc tataaaatta      360

taataatctt aataataata accatattaa tggaaggatg aggcccgatg tccttttttt      420

tgcctttcta ctatagtgct tacattgtgt ataaattctc gcggccgcgg atccctcgag      480

gccttaatta acatctgaat gtaaaatgaa cattaaaatg aattactaaa ctttacgtct      540

actttacaat ctataaactt tgtttaatca tataacgaaa tacactaata cacaatcctg      600

tacgtatgta atacttttat ccatcaagga ttgagaaaaa aaagtaatga ttccctgggc      660

cattaaaact tagaccccca agcttggata ggtcactctc tattttcgtt tctcccttcc      720

ctgatagaag ggtgatatgt aattaagaat aatatataat tttataataa aagaattcat      780

agcctcatga aatcagccat ttgcttttgt tcaacgatct tttgaaattg ttgttgttct      840

tggtagttaa gttgatccat cttggcttat gttgtgtgta tgttgtagtt attcttagta      900

tattcctgtc ctgagtttag tgaaacataa tatcgccttg aaatgaaaat gctgaaattc      960

gtcgacatac aatttttcaa actttttttt tttcttggtg cacggacatg tttttaaagg     1020

aagtactcta taccagttat tcttcacaaa tttaattgct ggagaataga tcttcaacgc     1080

tttaataaag tagtttgttt gtcaaggatg gcgtcataca aagaaagatc agaatcacac     1140

acttcccctg ttgctaggag acttttctcc atcatggagg aaaagaagtc taacctttgt     1200

gcatcattgg atattactga aactgaaaag cttctctcta ttttggacac tattggtcct     1260

tacatctgtc tagttaaaac acacatcgat attgtttctg attttacgta tgaaggaact     1320

gtgttgcctt tgaaggagct tgccaagaaa cataatttta tgatttttga agatagaaaa     1380

tttgctgata ttggtaacac tgttaaaaat caatataaat ctggtgtctt ccgtattgcc     1440

gaatgggctg acatcactaa tgcacatggt gtaacgggtg caggtattgt ttctggcttg     1500

aaggaggcag cccaagaaac aaccagtgaa cctagaggtt tgctaatgct tgctgagtta     1560

tcatcaaagg gttctttagc atatggtgaa tatacagaaa aaacagtaga aattgctaaa     1620

tctgataaag agtttgtcat tggttttatt gcgcaacacg atatgggcgg tagagaagaa     1680

ggttttgact ggatcattat gactccaggg gttggtttag atgacaaagg tgatgcactt     1740

ggtcaacaat atagaactgt tgatgaagtt gtaaagactg gaacggatat cataattgtt     1800

ggtagaggtt tgtacggtca aggaagagat cctatagagc aagctaaaag ataccaacaa     1860

gctggttgga atgcttattt aaacagattt aaatgattct tacacaaaga tttgatacat     1920

gtacactagt ttaaataagc atgaaaagaa ttacacaagc aaaaaaaaaa aaataaatga     1980

ggtactttac gttcacctac aaccaaaaaa actagataga gtaaaatctt aagatttaga     2040

aaaagttgtt taacaaaggc tttagtatgt gaatttttaa tgtagcaaag cgataactaa     2100

taaacataaa caaaagtatg gttttcttta tcagtcaaat cattatcgat tgattgttcc     2160

gcgtatctgc agatagcctc atgaaatcag ccatttgctt ttgttcaacg atcttttgaa     2220

attgttgttg ttcttggtag ttaagttgat ccatcttggc ttatgttgtg tgtatgttgt     2280

agttattctt agtatattcc tgtcctgagt ttagtgaaac ataatatcgc cttgaaatga     2340

aaatgctgaa attcgtcgac atacaatttt tcaaactttt tttttttctt ggtgcacgga     2400

catgttttta aaggaagtac tctataccag ttattcttca caaatttaat tgctggagaa     2460

tagatcttca acgccccggg ggatctggat ccgcggccgc gttaacgaaa gttccaaact     2520

ttatttataa tgtgtttatg tttgtatttt aatcactctt tatgacctat atatgaagct     2580

tttagcatta tcgcagcaag tataaatgga tgcatgtaaa ttccatagtt catatagtgc     2640

gatttggtga atttttgaaa tttttgctaa tggataatat actctatatt tttacactgt     2700

gtttactgat gcctcttccg aatttctttc tttcaccact caacccatga aaggcaagga     2760

acacatacat catgattaca ataatataga tatcggggta acaataacag ttcccagaag     2820

aaggaaacaa aaacgtacag gatctacaaa tagtcaaagc actgggtgga agaaattgtt     2880

atggctcaaa caaccttatg acgataacta cacagattcg agcttcttat cacaactgaa     2940

acgaaattca acggttgtaa agtactcgta tgtaaagcta gtcaatgatt tttccatcat     3000

tgtattgcat ctgtcgtcca ttatgtttgt tgttgttgta ttttatggga tctatcagtt     3060

aaattggaac ccgattaaac caacagtgat aagtacgatt tgtacactca ttggattcat     3120

tttttatgtt gtaacattga agataataag aaataaagaa ttgattgaac gagct          3175


<210>  41
<211>  3175
<212>  DNA
<213>  Artificial

<220>
<223>  PGI gene deletion construct

<400>  41
cgttcaatca attctttatt tcttattatc ttcaatgtta caacataaaa aatgaatcca       60

atgagtgtac aaatcgtact tatcactgtt ggtttaatcg ggttccaatt taactgatag      120

atcccataaa atacaacaac aacaaacata atggacgaca gatgcaatac aatgatggaa      180

aaatcattga ctagctttac atacgagtac tttacaaccg ttgaatttcg tttcagttgt      240

gataagaagc tcgaatctgt gtagttatcg tcataaggtt gtttgagcca taacaatttc      300

ttccacccag tgctttgact atttgtagat cctgtacgtt tttgtttcct tcttctggga      360

actgttattg ttaccccgat atctatatta ttgtaatcat gatgtatgtg ttccttgcct      420

ttcatgggtt gagtggtgaa agaaagaaat tcggaagagg catcagtaaa cacagtgtaa      480

aaatatagag tatattatcc attagcaaaa atttcaaaaa ttcaccaaat cgcactatat      540

gaactatgga atttacatgc atccatttat acttgctgcg ataatgctaa aagcttcata      600

tataggtcat aaagagtgat taaaatacaa acataaacac attataaata aagtttggaa      660

ctttcgttaa cgcggccgcg gatccctcga ggccttaatt aacatctgaa tgtaaaatga      720

acattaaaat gaattactaa actttacgtc tactttacaa tctataaact ttgtttaatc      780

atataacgaa atacactaat acacaatcct gtacgtatgt aatactttta tccatcaagg      840

attgagaaaa aaaagtaatg attccctggg ccattaaaac ttagaccccc aagcttggat      900

aggtcactct ctattttcgt ttctcccttc cctgatagaa gggtgatatg taattaagaa      960

taatatataa ttttataata aaagaattca tagcctcatg aaatcagcca tttgcttttg     1020

ttcaacgatc ttttgaaatt gttgttgttc ttggtagtta agttgatcca tcttggctta     1080

tgttgtgtgt atgttgtagt tattcttagt atattcctgt cctgagttta gtgaaacata     1140

atatcgcctt gaaatgaaaa tgctgaaatt cgtcgacata caatttttca aacttttttt     1200

ttttcttggt gcacggacat gtttttaaag gaagtactct ataccagtta ttcttcacaa     1260

atttaattgc tggagaatag atcttcaacg ctttaataaa gtagtttgtt tgtcaaggat     1320

ggcgtcatac aaagaaagat cagaatcaca cacttcccct gttgctagga gacttttctc     1380

catcatggag gaaaagaagt ctaacctttg tgcatcattg gatattactg aaactgaaaa     1440

gcttctctct attttggaca ctattggtcc ttacatctgt ctagttaaaa cacacatcga     1500

tattgtttct gattttacgt atgaaggaac tgtgttgcct ttgaaggagc ttgccaagaa     1560

acataatttt atgatttttg aagatagaaa atttgctgat attggtaaca ctgttaaaaa     1620

tcaatataaa tctggtgtct tccgtattgc cgaatgggct gacatcacta atgcacatgg     1680

tgtaacgggt gcaggtattg tttctggctt gaaggaggca gcccaagaaa caaccagtga     1740

acctagaggt ttgctaatgc ttgctgagtt atcatcaaag ggttctttag catatggtga     1800

atatacagaa aaaacagtag aaattgctaa atctgataaa gagtttgtca ttggttttat     1860

tgcgcaacac gatatgggcg gtagagaaga aggttttgac tggatcatta tgactccagg     1920

ggttggttta gatgacaaag gtgatgcact tggtcaacaa tatagaactg ttgatgaagt     1980

tgtaaagact ggaacggata tcataattgt tggtagaggt ttgtacggtc aaggaagaga     2040

tcctatagag caagctaaaa gataccaaca agctggttgg aatgcttatt taaacagatt     2100

taaatgattc ttacacaaag atttgataca tgtacactag tttaaataag catgaaaaga     2160

attacacaag caaaaaaaaa aaaataaatg aggtacttta cgttcaccta caaccaaaaa     2220

aactagatag agtaaaatct taagatttag aaaaagttgt ttaacaaagg ctttagtatg     2280

tgaattttta atgtagcaaa gcgataacta ataaacataa acaaaagtat ggttttcttt     2340

atcagtcaaa tcattatcga ttgattgttc cgcgtatctg cagatagcct catgaaatca     2400

gccatttgct tttgttcaac gatcttttga aattgttgtt gttcttggta gttaagttga     2460

tccatcttgg cttatgttgt gtgtatgttg tagttattct tagtatattc ctgtcctgag     2520

tttagtgaaa cataatatcg ccttgaaatg aaaatgctga aattcgtcga catacaattt     2580

ttcaaacttt ttttttttct tggtgcacgg acatgttttt aaaggaagta ctctatacca     2640

gttattcttc acaaatttaa ttgctggaga atagatcttc aacgccccgg gggatctgga     2700

tccgcggccg cgagaattta tacacaatgt aagcactata gtagaaaggc aaaaaaaagg     2760

acatcgggcc tcatccttcc attaatatgg ttattattat taagattatt ataattttat     2820

agctttatta taccttatac caagtactat atattaggta ctgcacagac aatgatgtag     2880

cgtctatctt ctcttcatct tgagagagtc tcttttgcgg gcagccgttg cattgtagag     2940

atcgtccgac tcgaatgaag gtgcggttcc cggaacagcc ggaacggtgg gggccagtgg     3000

ggaagaaacg ttctgttcgt acggactgtg gacatccagt ggggagaata ggccttggaa     3060

ctgtggatgt ttcaacgata gcgagagaga gttgagagtc tggagattgt ttgaactact     3120

gcccatgttt gtaattccgt tgttcgttga agatatagat ggcgagcgaa gggcc          3175


<210>  42
<211>  3420
<212>  DNA
<213>  Trypanosoma brucei

<400>  42
atggttgatg gtagatcttc agcttctatt gttgcagttg atccagaaag agcagcaaga       60

gaaagagatg ctgcagctag agctttgtta caagattctc cattgcacac taccatgcaa      120

tatgctacct ccggtttaga attgaccgtc ccttatgcat tgaaagttgt tgcatctgcc      180

gacaccttcg atagagctaa ggaagttgca gatgaagtcc ttagatgtgc ctggcaattg      240

gctgatacag tccttaactc ctttaaccca aactctgaag tctctcttgt tggtagactt      300

ccagtcggtc agaagcatca aatgtccgcc ccacttaaga gagttatggc ttgttgtcaa      360

agagtttaca attcctctgc tggttgtttc gacccatcca ccgccccagt tgcaaaggct      420

ttgcgtgaaa tcgctttagg caaggagaga aacaatgcct gtttggaggc tttaacacaa      480

gcatgcactt tgccaaactc tttcgtcatt gactttgaag caggtactat ctcacgtaaa      540

catgaacatg cttcacttga cttaggtggt gtttcaaagg gttacatcgt tgactatgtt      600

attgataaca ttaacgcagc tggtttccaa aatgtctttt tcgattgggg tggtgattgt      660

agagcctccg gtatgaatgc tagaaatacc ccttgggttg ttggtattac tagaccacca      720

tcattagata tgttaccaaa cccaccaaag gaagcatcct atatctctgt tatctcattg      780

gacaacgaag ctttggcaac ctccggtgat tacgagaatt tgatctacac agctgatgac      840

aagcctttaa cttgtactta cgattggaag ggcaaggaac ttatgaagcc atctcaatca      900

aacattgccc aagtttcagt taagtgctat tcagcaatgt acgctgacgc tttagccacc      960

gcttgtttca tcaaaagaga tccagccaag gttagacaat tgttagatgg ttggagatac     1020

gttagagata ctgtcagaga ttacagagtt tatgttagag aaaatgagag agtcgctaag     1080

atgtttgaaa ttgcaaccga agatgctgaa atgagaaaaa gacgtatctc taatactttg     1140

cctgcaagag tcatcgttgt cggtggcggt ttagcaggtt tatctgcagc aattgaagct     1200

gcaggctgcg gtgcacaagt cgttttgatg gaaaaggaag ctaagttagg tggtaactct     1260

gcaaaggcaa cctctggtat caatggttgg ggtactagag cccaagcaaa ggcttccatt     1320

gttgacggtg gcaagtattt cgaaagagat acttacaaat ctggtattgg tggtaatacc     1380

gacccagctt tagttaagac tctttccatg aagtctgctg acgctattgg ttggttaaca     1440

tcattaggtg ttcctttaac agtcttatca caattgggtg gtcattccag aaagagaact     1500

cacagagcac cagacaaaaa ggatggcacc ccattaccta ttggttttac cattatgaaa     1560

accttagaag atcacgtcag aggtaatctt tctggtagaa ttactatcat ggaaaactgt     1620

tccgttacct ctttactttc tgaaactaag gaaagaccag atggtactaa acaaatcaga     1680

gttaccggtg ttgagttcac tcaagcaggc tctggcaaaa ctaccatttt ggccgacgca     1740

gtcatcttgg ccactggtgg tttctctaac gacaagaccg cagactcttt gttgagagaa     1800

catgcccctc acttagttaa ctttcctaca actaacggtc cttgggcaac tggtgacggt     1860

gttaagcttg ctcaaagatt aggtgcacaa ttggtcgaca tggataaggt tcaattgcat     1920

ccaactggtt tgattaaccc aaaagatcca gctaatccaa caaagttttt gggtccagaa     1980

gctttaagag gttccggtgg tgtcttgtta aacaaacagg gtaaaagatt tgttaacgaa     2040

ttagatttgc gttctgttgt ttccaaggcc attatggaac aaggtgctga atacccaggc     2100

tctggtggtt ctatgttcgc atattgtgtc cttaatgcag ctgcacaaaa gttgtttggt     2160

gtctcttccc acgagttcta ctggaaaaag atgggtttgt tcgttaaggc tgatactatg     2220

agagatttgg cagcattgat tggttgtcca gtcgagtctg ttcaacaaac tttagaggaa     2280

tatgaaagat tatctatttc tcagagatcc tgtccaatca ctagaaaatc tgtttaccca     2340

tgtgttttgg gcactaaggg tccatactac gttgctttcg tcaccccatc tattcactat     2400

acaatgggtg gttgtttgat ttccccatca gcagaaattc agatgaaaaa cacctcctcc     2460

cgtgctccat tgtcccattc caaccctatc ttgggtttgt tcggtgctgg tgaagttact     2520

ggtggtgtcc acggtggcaa tagattaggt ggtaactcat tgttagaatg tgttgtcttt     2580

ggtagaattg ctggtgatag agcttctacc attttgcaga gaaagtcctc cgcattatct     2640

ttcaaggtct ggactaccgt tgttttgaga gaagttagag aaggtggcgt ctatggtgcc     2700

ggttcaagag ttttgagatt caacttgcct ggtgctttac aaagatccgg tttgtccttg     2760

ggtcaattca tcgcaatcag aggtgactgg gatggtcaac aattgattgg ttactattcc     2820

ccaattacat tgccagatga cttgggtatg attgacattt tggctagatc cgataaaggt     2880

actttaagag aatggatttc tgctttagaa ccaggcgacg ctgttgagat gaaagcatgc     2940

ggtggtttag tcatcgagag aagattgtca gataagcact ttgtctttat gggtcacatc     3000

attaacaagt tatgtttgat cgctggtggt acaggcgttg cacctatgtt acaaatcatt     3060

aaggcagcat tcatgaaacc ttttatcgat accttagaat ctgtccatct tatctatgct     3120

gcagaagatg ttaccgagtt aacttataga gaagttttag aggagcgtag aagagagtct     3180

cgtggcaagt tcaaaaagac ctttgttttg aacagacctc caccactttg gactgatggt     3240

gttggtttca tcgatagagg tatcttaact aatcatgtcc aaccaccatc cgataacctt     3300

ttggttgcaa tctgtggtcc acctgtcatg cagcgtattg ttaaggccac cttaaagact     3360

ttgggttaca atatgaatct tgttagaaca gttgacgaaa cagaaccatc cggttcctaa     3420


<210>  43
<211>  3420
<212>  DNA
<213>  Trypanosoma cruzi

<400>  43
atggctgacg gtagatcctc tgcatctgtt gttgcagttg atccagaaaa ggctgcaaga       60

gaaagagatg aagcagctcg tgctttgtta agagactctc cattacaaac tcatcttcag      120

tacatgacta atggtttaga gttgactgtc ccattcacct taaaggttgt cgctgaagca      180

gttgcatttt ccagagcaaa ggaagttgct gacgaagttt tgaggtcagc ctggcatctt      240

gcagacaccg tcttgaacaa ctttaaccct aactccgaga tttctatgat tggtagatta      300

ccagttggtc aaaaacatac aatgtccgct acattgaagt ctgttatcac atgctgtcag      360

catgttttca attcatccag aggtgttttt gatccagcta ctggtcctat cattgaagct      420

ttaagagcta aggttgctga gaaagcctct gtttctgatg aacagatgga gaagttgttt      480

cgtgtttgta acttctcttc ctcattcatc gttgatttgg aaatgggtac tattgccaga      540

aaacacgaag atgcaagatt tgacttaggt ggtgtttcca agggttacat cgttgactac      600

gttgttgaaa gattgaacgc tgctggtatt gtcgatgtct acttcgaatg gggtggtgac      660

tgtagagctt ccggtactaa cgcaagacgt accccatgga tggttggtat cattagacct      720

ccatctttag aacaattgag aaacccacca aaagatccat cctacattag ggttttacca      780

cttaacgatg aagcactttg tacctctggt gactatgaga atttgaccga aggctctaac      840

aaaaagttgt atacatccat tttcgattgg aaaaagagat ccttgttgga accagttgaa      900

tcagaattgg cccaagtttc cattagatgt tattctgcca tgtatgcaga cgcattagca      960

acagcttctc ttatcaagag agatatcaaa aaggttagac aaatgttgga agattggaga     1020

cacgtccgta atagggttac taactatgtt acctatacca gacaaggtga aagagtcgca     1080

cgtatgtttg aaattgctac tgataacgct gagattagga aaaagagaat tgcaggctct     1140

ttacctgcta gggttattgt tgtcggtggt ggtttagctg gtttgtctgc agcaattgaa     1200

gcaactgcat gtggtgccca agttatcctt ttagaaaagg aacctaaagt tggtggtaat     1260

tccgcaaagg ctacatctgg tatcaacggt tggggtacta gagcacaagc tgaacaagat     1320

gtctacgact ctggcaagta cttcgaaaga gatacacaca aatctggttt aggtggttct     1380

accgatccag gcttagttcg tactttatca gtcaagtctg gtgacgctat ttcatggtta     1440

tcttctcttg gtgttccatt aactgtcttg tcacaattag gcggtcattc cagaaaaagg     1500

actcacagag cccctgataa ggcagatggt actccagttc caattggttt caccattatg     1560

caaaccttag aacagcatgt tagaaccaag ttagcagaca gagttactat catggagaat     1620

accaccgtta cctccttgct ttctaagtcc agagttagac atgatggtgc aaagcaagtt     1680

agagtctacg gtgttgaagt cttacaagac gaaggtgtcg tttctcgtat cttggccgat     1740

gctgtcattt tggcaacagg tggtttctcc aatgacaaaa ccccaaactc cttattgcaa     1800

gagttcgctc cacaattgtc aggttttcca acaaccaacg gtccatgggc tactggcgat     1860

ggtgttaagt tagcaagaga acttggtgtc aagttggttg atatggataa ggtccaactt     1920

catccaactg gtttgattga ccctaaggac ccagcaaatc caaccaaata cttaggtcca     1980

gaagcattga gaggttctgg tggtgtcttg ttaaacaaaa agggtgaaag atttgtcaat     2040

gagttggact tgcgttccgt cgtttcaaat gctatcattg aacaaggtga tgaatatcca     2100

gatgccggtg gttccaagtt cgccttctgt gttttgaatg atgcagcagt taagttattc     2160

ggtgtcaact cccacggttt ctactggaag agacttggtt tgtttgttaa ggctgatacc     2220

gttgaaaagt tagccgcatt gatcggttgc ccagtcgaaa atgttagaaa cacattaggt     2280

gattatgagc aattgtccaa ggaaaacaga caatgtccaa agactagaaa agttgtctat     2340

ccatgtgttg ttggtccaca aggtccattc tatgttgctt ttgttacccc atctattcac     2400

tataccatgg gtggttgttt gatctcacca tctgctgaga tgcaattgga agagaacact     2460

acctccccat ttggtcacag aaggcctatc ttcggtcttt tcggtgccgg tgaagttact     2520

ggtggtgtcc atggtggtaa cagattaggt ggcaactctt tgttggagtg tgttgttttt     2580

ggtagaatcg ctggtgatag agctgcaacc attttgcaaa agaaaccagt tccactttcc     2640

tttaagactt ggaccaccgt cattttgaga gaggtccgtg aaggtggcat gtacggtact     2700

ggttcaagag tcttaagatt caatttgcca ggtgctttac aaagatctgg tttgcaattg     2760

ggtcaattca tcgctattag aggcgaatgg gatggtcaac aattgattgg ctactattcc     2820

ccaatcactt tgccagacga tttgggtgtc atcggcattt tggctagatc cgataagggt     2880

actttgaagg aatggatttc tgctttggaa cctggtgatg cagttgagat gaagggttgt     2940

ggcggtttag ttattgaaag gagattctct gaaagatact tgtacttttc tggtcacgct     3000

ttgaaaaagt tatgccttat tgctggtggt actggtgtcg caccaatgtt acaaatcatt     3060

agagcagcat tgaaaaagcc attccttgag aatatcgaat caattagact tatctatgct     3120

gctgaggacg tttctgagtt gacatacagg gaattgttag aacatcacca aagagattct     3180

aagggcaagt ttagatccat cttcgttttg aatagaccac ctccaatttg gactgatggt     3240

gttggcttta tcgacaaaaa gttgttatct tcatccgttc agccacctgc taaggatttg     3300

ttagtcgcca tttgtggtcc tcctatcatg caacgtgttg tcaagacttg tcttaagtca     3360

ttaggttatg atatgcagtt agtcagaaca gttgatgaag tcgaaactca aaactcctaa     3420


<210>  44
<211>  3435
<212>  DNA
<213>  Leishmania braziliensis

<400>  44
atggctgatg gtaaaacctc tgcttccgtt gttgctgtcg acccagagcg tgcagcaaag       60

gagagagatg cagcagcaag agcaatgtta caagacggtg gtgtttctcc agttggtaaa      120

gctcagttgt tgaaaaaggg tttggcatat gctgtccctt acacccttaa gattgttgtt      180

gcagatccta aagctatgga aaagaccacc gcagacgttg agaaggtcct tcaaaccgca      240

ttccaagtcg ttgacacttt gttaaacaat ttcaacgaaa actccgaggt ttctcgtatc      300

aacagaatgc cagtcggtga ggaacaccaa atgtctgctg cattgaagag agttatgggt      360

tgctgtcagc gtgtttacaa ttcatctcgt ggtgcttttg acccagctgt tggtccattg      420

gtcagagaat tgagggaagc tgcaagagaa ggcagaactt taccagcaga aaggattaac      480

gctttgttat ccaagtgtac cttgaatatc tccttttcca ttgatttgaa cagaggtact      540

attgccagaa aacacgcaga tgcaatgttg gatttgggtg gtgtcaataa gggttatggt      600

gttgattatg ttgtcgaaca tttgaacaat ttgggttatg atgatgtctt tttcgaatgg      660

ggtggtgatg ttagagcatc tggcaaaaac ccatcaaacc aacattgggt tgttggtatt      720

gctagaccac cagcacttgc tgatatcaga accgttgttc cacaagacaa gcaatccttc      780

atcagagttg tttgtcttaa tgatgaagca attgccacct ctggtgatta cgaaaatctt      840

gtcgaaggtc ctggttctaa ggtttactcc tctaccttca acgcaacctc taagtcctta      900

ttggaaccaa ccgaaaccaa tatcgcacaa gtctctgtta agtgttactc atgcatgtat      960

gcagacgcat tggctaccgc tgccttattg aaaaacaatc caactgctgt tcgtagaatg     1020

ttagataact ggagatatgt tcgtgatact gttaccgact atacaaccta ttccagagaa     1080

ggtgaaagag ttgcaaagat gtttgagatt gcaaccgaag ataaggaaat gagagctaag     1140

agaatttccg gttccttgcc agcaagagtc attatcgtcg gtggtggttt agctggttgt     1200

tctgcagcta ttgaagcagt caactgtggt gctcaagtca ttttgttaga aaaggaagcc     1260

aagattggtg gcaactccgc aaaggctacc tctggtatca acgcctgggg tactagagcc     1320

caggctaaac aaggtgttat ggatggtggc aagtttttcg agagagacac ccatagatcc     1380

ggtaaaggtg gtcactgtga tccttgtttg gttaagacac tttccgttaa gtcatcagac     1440

gcagttaagt ggttgtctga attgggtgtt ccattaaccg tcttatccca attaggtggt     1500

gcatccagaa agaggtgtca tagagcccca gataagtctg atggtactcc tgttccaatt     1560

ggttttacaa tcatgaaaac attagaaaat cacatcatta acgatctttc tcaccaagtt     1620

actgttatga ctggtatcaa ggttactggt ttggagtcca cttctcacgc tcgtccagat     1680

ggtgttttag ttaagcacgt tactggtgtt agattgattc aaggtgatgg ccaatccaga     1740

gttttgaatg ctgatgccgt tatcttagca actggtggtt tctccaatga ccatactgct     1800

aactctttac ttcaacaata cgctccacaa ctttcatcct ttccaaccac taatggtgtt     1860

tgggccactg gtgacggtgt caaggcagct agagaattag gtgttgagtt ggttgacatg     1920

gataaggtcc aattgcatcc aacaggtttg ttagatccaa aggacccatc caacaggact     1980

aagtacttgg gtccagaagc tttaaggggt tcaggcggtg tcttgttaaa caaaaacggt     2040

gaacgtttcg tcaacgaact tgatttgaga tctgtcgttt ctcaagccat tatcgaacaa     2100

aacaacgttt accctggttc tggtggttcc aagtttgctt actgcgtttt gaacgaagca     2160

gcagctaagt tgttcggcaa aaactttttg ggtttctatt ggcatagatt aggtcttttt     2220

gaaaaggttg aagatgttgc tggtttagcc aaattgatcg gttgtccaga ggaaaatgtt     2280

accgctacat tgaaggaata caaggaattg tcctccaaaa agcttcatgc ctgtccttta     2340

accaacaaaa acgtctttcc ttgcacttta ggtactgaag gcccttacta tgttgctttc     2400

gtcacacctt caattcacta cacaatgggt ggttgtttga tctccccttc agcagaaatg     2460

cagaccattg ataacactgg tgtcacacca gttcgtagac caatcttggg cttattcggt     2520

gctggtgaag ttactggtgg tgtccatggt ggtaacagat tgggtggtaa ttccttattg     2580

gaatgtgttg tctttggtag aattgctggt gatagagccg ctaccatttt gcaaaagaag     2640

aatgctggtt tatcaatgac tgagtggtct acagttgtct taagagaagt cagagaaggc     2700

ggtgtttacg gtactggttc tcgtgtcctt agattcaata tgccaggtgc cttacaaaag     2760

actggcttag cattgggtca attcatcgca atgagaggtg attgggatgg tcaacagtta     2820

ttgggttact attctccaat tacattacca gacgacattg gtgttattgg tatcttagct     2880

agagctgaca aaggtagatt agctgaatgg atttctgcat tacaaccagg tgatgctgtt     2940

gagatgaagg catgtggcgg tttgattatc catagaagat tcgctgctag acacttgttt     3000

ttccgttctc acaagattag aaagcttgct cttattggtg gtggtactgg tgttgcacca     3060

atgttgcaaa ttgtcagggc tgcagtcaaa aagccatttg ttgactctat tgagtctatt     3120

cagttcatct atgcagctga agatgtctcc gaacttactt atagaacttt gttggaatca     3180

tatgaaaagg aatacggttc tggcaaattc aagtgtcatt tcgtcttgaa taacccacca     3240

tcacaatgga ccgagggcgt tggtttcgtt gatactgctt tgttgcgttc tgccgttcaa     3300

gcaccttcta acgacttgtt agtcgctatt tgtggcccac caatcatgca aagagcagtc     3360

aaatcagcct taaagggttt aggttacaat atgaatttgg ttagaacagt tgatgaacca     3420

gaaccattgt cttaa                                                      3435


<210>  45
<211>  3537
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  45
atgtcgcaaa gaaaattcgc cggcttgaga gataacttca atctcttggg tgaaaagaac       60

aaaatattgg tggctaatag aggagaaatt ccaatcagaa tttttcgtac cgctcatgaa      120

ctgtctatgc agacggtagc tatatattct catgaagatc gtctttcaac gcacaaacaa      180

aaggctgacg aagcatacgt cataggtgaa gtaggccaat atacccccgt cggcgcttat      240

ttggccattg acgaaatcat ttccattgcc caaaaacacc aggtagattt catccatcca      300

ggttatgggt tcttgtctga aaattcggaa tttgccgaca aagtagtgaa ggccggtatc      360

acttggattg gccctccagc tgaagttatt gactccgtgg gtgataaggt ctcagctaga      420

aacctggcag caaaagctaa tgtgcccacc gttcctggta caccaggtcc tatagaaact      480

gtagaggaag cacttgactt cgtcaatgaa tacggctacc cggtgatcat taaggccgcc      540

tttggtggtg gtggtagagg tatgagagtc gttagagaag gtgacgacgt ggcagatgcc      600

tttcaacgtg ctacctccga agcccgtact gccttcggta atggtacctg ctttgtggaa      660

agattcttgg acaagccaaa gcatattgaa gttcaattgt tggccgataa ccacggaaac      720

gtggttcatc ttttcgaaag agactgttcc gtgcagagaa gacaccaaaa ggttgtcgaa      780

gtggccccag caaagacttt accccgtgaa gtccgtgacg ccattttgac agatgcagtt      840

aaattggcca aagagtgtgg ctacagaaat gcgggtactg ctgaattctt ggttgataac      900

caaaatagac actatttcat tgaaattaat ccaagaatcc aagtggaaca taccatcaca      960

gaagaaatta ccggtataga tattgtggcg gctcagatcc aaattgcggc aggtgcctct     1020

ctaccccagc tgggcctatt ccaggacaaa attacgactc gtggctttgc cattcagtgc     1080

cgtattacca cggaagaccc tgctaagaac ttccaaccag ataccggtag aatagaagtg     1140

taccgttctg caggtggtaa tggtgttaga ctggatggtg gtaacgccta tgcaggaaca     1200

ataatctcac ctcattacga ctcaatgctg gtcaaatgct catgctccgg ttccacctac     1260

gaaatcgttc gtagaaaaat gattcgtgca ttaatcgagt tcagaattag aggtgtcaag     1320

accaacattc ccttcctatt gactcttttg accaatccag tatttattga gggtacatac     1380

tggacgactt ttattgacga caccccacaa ctgttccaaa tggtttcatc acaaaacaga     1440

gcccaaaaac ttttacatta cctcgccgac gtggcagtca atggttcatc tatcaagggt     1500

caaattggct tgccaaaatt aaaatcaaat ccaagtgtcc cccatttgca cgatgctcag     1560

ggcaatgtca tcaacgttac aaagtctgca ccaccatccg gatggaggca agtgctacta     1620

gaaaaggggc cagctgaatt tgccagacaa gttagacagt tcaatggtac tttattgatg     1680

gacaccacct ggagagacgc tcatcaatct ctacttgcaa caagagtcag aacccacgat     1740

ttggctacaa tcgctccaac aaccgcacat gcccttgcag gtcgtttcgc cttagaatgt     1800

tggggtggtg ccacattcga tgttgcaatg agatttttgc atgaggatcc atgggaacgt     1860

ttgagaaaat taagatctct ggtgcctaat attccattcc aaatgttatt gcgtggtgcc     1920

aatggtgtgg cttattcttc attgcctgac aatgctattg accatttcgt caagcaagcc     1980

aaggataatg gtgttgatat atttagagtc tttgatgcct taaatgactt ggaacaattg     2040

aaggtcggtg tagatgctgt gaagaaggca ggtggtgttg tagaagccac tgtttgtttc     2100

tctggggata tgcttcagcc aggcaagaaa tacaatttgg attactactt ggaaattgct     2160

gaaaaaattg tccaaatggg cactcatatc ctgggtatca aagatatggc aggtaccatg     2220

aagccagcag ctgccaaact actgattgga tctttgaggg ctaagtaccc tgatctccca     2280

atacatgttc acactcacga ttctgcaggt actgctgttg catcaatgac tgcgtgtgct     2340

ctggcgggcg ccgatgtcgt tgatgttgcc atcaactcaa tgtctggttt aacttcacaa     2400

ccatcaatca atgctctgtt ggcttcatta gaaggtaata ttgacactgg tattaacgtt     2460

gagcatgtcc gtgaactaga tgcatattgg gcagagatga gattgttata ctcttgtttc     2520

gaggctgact tgaagggccc agatccagaa gtttatcaac atgaaatccc aggtggtcaa     2580

ttgacaaact tgttgtttca agcccaacaa ttgggtcttg gagaacaatg ggccgaaaca     2640

aaaagagctt acagagaagc caattattta ttgggtgata ttgtcaaagt taccccaact     2700

tcgaaggtcg ttggtgatct ggcacaattt atggtctcca ataaattaac ttccgatgat     2760

gtgagacgcc tggctaattc tttggatttc cctgactctg ttatggattt cttcgaaggc     2820

ttaatcggcc aaccatacgg tgggttccca gaaccattta gatcagacgt tttaaggaac     2880

aagagaagaa agttgacttg tcgtccaggc ctggaactag agccatttga tctcgaaaaa     2940

attagagaag acttgcagaa tagatttggt gatgttgatg agtgcgacgt tgcttcttat     3000

aacatgtacc caagagttta tgaagacttc caaaagatga gagaaacgta tggtgattta     3060

tctgtattgc caacaagaag ctttttgtct ccactagaga ctgacgaaga aattgaagtt     3120

gtaatcgaac aaggtaaaac gctaattatc aagctacagg ctgtgggtga tttgaacaaa     3180

aagaccggtg aaagagaagt ttactttgat ttgaatggtg aaatgagaaa aattcgtgtt     3240

gctgacagat cacaaaaagt ggaaactgtt actaaatcca aagcagacat gcatgatcca     3300

ttacacattg gtgcaccaat ggcaggtgtc attgttgaag ttaaagttca taaaggatca     3360

ctaataaaga agggccaacc tgtagccgta ttaagcgcca tgaaaatgga aatgattata     3420

tcttctccat ccgatggaca agttaaagaa gtgtttgtct ctgatggtga aaatgtggac     3480

tcttctgatt tattagttct attagaagac caagttcctg ttgaaactaa ggcataa        3537


<210>  46
<211>  3528
<212>  DNA
<213>  Kluyveromyces marxianus

<400>  46
atgtctaccc aaaacgatct ggccgggttg cgtgataact cgaacctatt aggtgaaaag       60

aacaagattc ttgttgccaa ccgtggtgaa attccaatta gaatctttag aacggctcat      120

gaactttcga tgaagactgt tgcgatctat tcgcacgagg atagactatc tatgcacaga      180

ttgaaggcag acgaagctta cgttattggt gagccaggaa aatacactcc agttggtgcg      240

tatttggcga tcgatgagat tatcaagatt gctcaattgc acggagtgag cttcatccac      300

cctggttatg ggttcttatc ggaaaactct gagtttgcca agaaggtggc cgactctggt      360

atcacgtggg ttggtcctcc agccgatgtg atcgatgctg ttggtgacaa ggtttctgcc      420

agaaacttgg ccgagagagc ggatgttcca gtggttccag gtacgcctgg tccaatagag      480

acagttgaag aagcagttga atttgtggag aagtacggat acccagtcat catcaaggct      540

gccttcggtg gtggtggtcg tggtatgaga gttgttcgtg aaggtgatga tatcgccgat      600

gctttccaaa gagccaagtc cgaagctgtt actgctttcg gtaacggtac ttgtttcgtt      660

gaaagattct tggacaagcc aaagcacatc gaagttcagt tgttggctga tcactacggt      720

aatgtcatcc atctattcga aagagactgt tctgtgcaaa gaagacatca aaaggtcgtt      780

gaagtagcgc cagccaagac tttgccagag agcgtgcgta atgcaatctt gactgacgct      840

gtcaagttgg ctaaggaggc aggatacaga aatgctggta ccgctgaatt tttggtcgac      900

aaccaaaaca gacactactt tattgaaatc aacccaagaa ttcaagtcga acataccatc      960

accgaagaaa ttaccggtat cgacattgtc gccgcacaaa ttcaaatcgc agcaggtgct     1020

tccttggaac aattgggact attgcaagat agaatcacca cccgtggttt cgctattcaa     1080

tgtcgtatca ctactgaaga tccttccaag aacttccagc cagatactgg tcgtatcgat     1140

gtttaccgtt ccgctggtgg taacggtgtc agattggatg gtggtaacgc attcgctggt     1200

tcggtcattt cacctcatta tgattccatg ttggtcaaat gttcttgttc cggttccact     1260

tacgaaatcg ttcgtcgtaa gatgttgcgt gccttgatcg aattcagaat cagaggtgtg     1320

aagacaaaca ttccattctt gctaacgttg ttgactcatc ctgtgttcaa gtccggtgac     1380

tactggacta ccttcatcga tgacactcca caattgttcg aaatggtttc ttctcaaaac     1440

agagcacaaa aactattgca ctacttggcc gatcttgccg ttaacggttc atcgatcaag     1500

ggtcaaattg gtctaccaaa gttaaagact catcctacta tcccacattt gcataaggcc     1560

gatggctcca ttctagatgt gtctgccaag cctcctgccg ggtggagaga tgttctattg     1620

caacacggcc cagaagaatt tgcaaagcaa gttagaaagt tcaagggtac tttgctaatg     1680

gacaccacct ggagagatgc tcatcaatct ctattggcca ctagagtcag aacttacgat     1740

ttggctgcca tcgctccaac tactgctcat gctttgagcg gtgctttcgc tttggaatgt     1800

tggggtggtg ccactttcga tgtctccatg agattcttgc acgaagatcc atgggaacgt     1860

ttgagaactt tgagaaagtt ggttcctaac attccattcc aaatgttgct acgtggtgcc     1920

aacggtgttg catactcttc tctaccagat aacgctatcg accactttgt caagcaagca     1980

aaggataacg gtgttgacat tttcagagtc ttcgatgctc taaacgattt ggagcaattg     2040

actgtcggtg ttgacgctgt caagaaggct ggtggtgttg tcgaagctac catttgttac     2100

tccggtgaca tgctagcacc aggtaagaag tacaaccttg actactactt ggacattgtt     2160

gaacaagtgg ttaagagagg tacccatatt cttggtatca aggatatggc aggtactttg     2220

aagccatctg ctgctaagct cttgatcggt tctatcagaa caaagtaccc tgacttgcca     2280

attcacgtcc atacccatga ctccgccggt accggtgttg cttccatggc tgcatgtgct     2340

ttcgctggtg ctgatgttgt tgatgttgca accaactcta tgtctggtat gacttctcaa     2400

ccatctgtca atgcactatt ggctgctctt gatggtgaaa tcgactgtaa tgtcaacgtc     2460

agctacatca gtcagctaga tgcttactgg gctgaaatga gactattgta ctcatgtttc     2520

gaagccgact tgaagggtcc tgatccagaa gtttacgtcc atgaaattcc aggtggtcaa     2580

ttgaccaact tgctcttcca agcccaacaa ttgggtcttg gtgagcaatg ggctgaaacc     2640

aagagagctt accgtgaagc aaacctgttg ttgggtgatg ttgttaaggt cactccaaca     2700

tccaaggttg tcggtgattt ggctcaattc atggtcacta acaagttgac ctcggatgat     2760

gttaagagat tagcttcatc tttggatttc ccagactccg tcatggactt ctttgaaggt     2820

ttaatcggtc aaccatacgg tggtttccca gaacctctaa gatctgatgt tttgaagaac     2880

aagagaagaa agttgaccaa gagaccaggt ttggaattgg ctccattcga tttggaaggc     2940

attaaggaag atttgactaa cagatttggt gacattgacg actgtgatgt tgcttcttac     3000

aacatgtatc caaaggtcta cgaagatttc cgtaagatca gagaaaagta cggtgatcta     3060

tctgttttgc caaccaagaa cttcttgtct ccaccttcaa tcggtgaaga aatcgtcgtt     3120

acaattgaac aaggtaagac tttgatcatt aagccacaag ctattggtga tttgaacaag     3180

gagactggta tcagagaagt ttacttcgaa ttgaacggtg aattgagaaa ggtctctgtt     3240

gctgacagat ctcaaaaggt tgaaacgatc tccaagccaa aggctgacgc ccacgatcca     3300

ttccaagttg gttctccaat ggcaggtgtt gttgtcgaag tcaaggtaca caagggttct     3360

ttgatctcca agggccaacc agtcgctgtc ctaagtgcca tgaagatgga aatggttatc     3420

tcctccccat ctgatggtca agtcaaggaa gtgcttgtca aggatggtga aaacgttgac     3480

gcttctgact tgctcgttgt tttggaagaa gctccagcta aagaataa                  3528


<210>  47
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  47
gcaactgatg ttcacgaatg cg                                                22


<210>  48
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  48
ttgccgttgc agcaaatctc                                                   20


<210>  49
<211>  2652
<212>  DNA
<213>  Escherichia coli

<400>  49
atgaacgaac aatattccgc attgcgtagt aatgtcagta tgctcggcaa agtgctggga       60

gaaaccatca aggatgcgtt gggagaacac attcttgaac gcgtagaaac tatccgtaag      120

ttgtcgaaat cttcacgcgc tggcaatgat gctaaccgcc aggagttgct caccacctta      180

caaaatttgt cgaacgacga gctgctgccc gttgcgcgtg cgtttagtca gttcctgaac      240

ctggccaaca ccgccgagca ataccacagc atttcgccga aaggcgaagc tgccagcaac      300

ccggaagtga tcgcccgcac cctgcgtaaa ctgaaaaacc agccggaact gagcgaagac      360

accatcaaaa aagcagtgga atcgctgtcg ctggaactgg tcctcacggc tcacccaacc      420

gaaattaccc gtcgtacact gatccacaaa atggtggaag tgaacgcctg tttaaaacag      480

ctcgataaca aagatatcgc tgactacgaa cacaaccagc tgatgcgtcg cctgcgccag      540

ttgatcgccc agtcatggca taccgatgaa atccgtaagc tgcgtccaag cccggtagat      600

gaagccaaat ggggctttgc cgtagtggaa aacagcctgt ggcaaggcgt accaaattac      660

ctgcgcgaac tgaacgaaca actggaagag aacctcggct acaaactgcc cgtcgaattt      720

gttccggtcc gttttacttc gtggatgggc ggcgaccgcg acggcaaccc gaacgtcact      780

gccgatatca cccgccacgt cctgctactc agccgctgga aagccaccga tttgttcctg      840

aaagatattc aggtgctggt ttctgaactg tcgatggttg aagcgacccc tgaactgctg      900

gcgctggttg gcgaagaagg tgccgcagaa ccgtatcgct atctgatgaa aaacctgcgt      960

tctcgcctga tggcgacaca ggcatggctg gaagcgcgcc tgaaaggcga agaactgcca     1020

aaaccagaag gcctgctgac acaaaacgaa gaactgtggg aaccgctcta cgcttgctac     1080

cagtcacttc aggcgtgtgg catgggtatt atcgccaacg gcgatctgct cgacaccctg     1140

cgccgcgtga aatgtttcgg cgtaccgctg gtccgtattg atatccgtca ggagagcacg     1200

cgtcataccg aagcgctggg cgagctgacc cgctacctcg gtatcggcga ctacgaaagc     1260

tggtcagagg ccgacaaaca ggcgttcctg atccgcgaac tgaactccaa acgtccgctt     1320

ctgccgcgca actggcaacc aagcgccgaa acgcgcgaag tgctcgatac ctgccaggtg     1380

attgccgaag caccgcaagg ctccattgcc gcctacgtga tctcgatggc gaaaacgccg     1440

tccgacgtac tggctgtcca cctgctgctg aaagaagcgg gtatcgggtt tgcgatgccg     1500

gttgctccgc tgtttgaaac cctcgatgat ctgaacaacg ccaacgatgt catgacccag     1560

ctgctcaata ttgactggta tcgtggcctg attcagggca aacagatggt gatgattggc     1620

tattccgact cagcaaaaga tgcgggagtg atggcagctt cctgggcgca atatcaggca     1680

caggatgcat taatcaaaac ctgcgaaaaa gcgggtattg agctgacgtt gttccacggt     1740

cgcggcggtt ccattggtcg cggcggcgca cctgctcatg cggcgctgct gtcacaaccg     1800

ccaggaagcc tgaaaggcgg cctgcgcgta accgaacagg gcgagatgat ccgctttaaa     1860

tatggtctgc cagaaatcac cgtcagcagc ctgtcgcttt ataccggggc gattctggaa     1920

gccaacctgc tgccaccgcc ggagccgaaa gagagctggc gtcgcattat ggatgaactg     1980

tcagtcatct cctgcgatgt ctaccgcggc tacgtacgtg aaaacaaaga ttttgtgcct     2040

tacttccgct ccgctacgcc ggaacaagaa ctgggcaaac tgccgttggg ttcacgtccg     2100

gcgaaacgtc gcccaaccgg cggcgtcgag tcactacgcg ccattccgtg gatcttcgcc     2160

tggacgcaaa accgtctgat gctccccgcc tggctgggtg caggtacggc gctgcaaaaa     2220

gtggtcgaag acggcaaaca gagcgagctg gaggctatgt gccgcgattg gccattcttc     2280

tcgacgcgtc tcggcatgct ggagatggtc ttcgccaaag cagacctgtg gctggcggaa     2340

tactatgacc aacgcctggt agacaaagca ctgtggccgt taggtaaaga gttacgcaac     2400

ctgcaagaag aagacatcaa agtggtgctg gcgattgcca acgattccca tctgatggcc     2460

gatctgccgt ggattgcaga gtctattcag ctacggaata tttacaccga cccgctgaac     2520

gtattgcagg ccgagttgct gcaccgctcc cgccaggcag aaaaagaagg ccaggaaccg     2580

gatcctcgcg tcgaacaagc gttaatggtc actattgccg ggattgcggc aggtatgcgt     2640

aataccggct aa                                                         2652


<210>  50
<211>  2643
<212>  DNA
<213>  Mannheimia succiniproducens

<400>  50
atgacagaag aatatttaat gatgcgtaat aacatcaata tgctggggcg ctttttgggc       60

gaaactattc aggaggcgca aggtgacgat attctcgaac tgattgaaaa tatccgcgta      120

ctgtcccgca attcccgtag cggcgatgac aaagcccggg cggcattatt agacaccctt      180

tccactattt cggcggataa tattattccg gttgcccgcg ctttcagcca gtttctgaac      240

ctgacaaatg tggcggaaca atatcaaacc atgtctcgct cccatgaaga taaggtttct      300

gcggaacgtt ccactgctgc gctgttcgcc cgcctgaaag aacaacatgt ttctcaggaa      360

gaaatcatta aaaccgtaca gaaactgttg attgaaatcg tccttaccgc tcacccgacg      420

gaagttaccc gccgttcatt aatgcacaaa caggttgaaa tcaacaaatg tctggctcag      480

ctggatcata cggatttaac cgccgaagaa caaaaaaata ttgagtataa attacttcgt      540

cttatcgccg aagcctggca taccaatgaa atccgtacca atcggccgac acctctggaa      600

gaagccaaat ggggttttgc cgttatcgaa aacagtttat gggaaggttt gcccgccttt      660

atccgcaaac ttaacgatgc cgccgtcgaa catttaaatt atgctttgcc ggtagacctc      720

acaccggtac gcttctcttc ctggatgggc ggtgaccgtg acggcaaccc cttcgttacc      780

gcaaaaatta cccgggaagc gctgcaactt gcgcgctgga aagcggcgga tttattttta      840

accgatattc aggaactctg cgacgagttg tcaatgacac aatgcactgc ggaattccga      900

gaaaaatacg gtgatcattt agaaccctat cgtgtagttg tgaaggattt acgcagcaaa      960

ttaaaaaata cgctggatta ttacaacgat atacttgcgg gtcgcattcc gccgtttaaa     1020

caagatgaaa tcatcagtga agaccaacaa ctctggcaac cgctttatga ctgttatcaa     1080

tccctaaccg cctgcggtat gcgtattatt gccaatggat tattgctgga taccttacgc     1140

cgcgttcgtt gtttcggcgt cacattactg cgtttagata tccgtcagga aagcacccgc     1200

catagcgacg ccatcggcga aattacccgc tacatcggtt taggcgatta cagccaatgg     1260

acagaagatg acaaacaagc cttcctgatc cgggaattaa gttcccgtcg tccgctaatt     1320

ccccataact ggacgccttc ggaacacact cgggaaattt tagacacctg taaagtcatt     1380

gcaaaacagc cggaaggcgt tatttcctgc tatatcattt ccatggcgcg caccgcttcc     1440

gatgttttgg cggtgcattt attattgaaa gaagcgggca tttcatacca tctgccggta     1500

gttcctctat ttgaaacatt ggacgacctg gacgcttcta aagaagtgat gacgcaactg     1560

tttaacgtag gctggtatcg cggcgtaatc aaaaaccgcc aaatgatcat gatcggctat     1620

tccgatagcg ccaaagatgc gggcatgatg gcggcctcat gggcgcaata ccgggcgcag     1680

gacgctttag tcaaactttg cgaacaaacc ggcatcgaac ttaccctctt ccacggccgc     1740

ggcggcaccg taggacgtgg cggtgcaccg gctcacgccg cattattatc ccaaccgcca     1800

cgttctctga aaaacggctt acgggtaacc gaacaagggg aaatgatccg cttcaaactg     1860

ggattaccgg ctatcgccgc agaaagtctg gatctctacg ccagcgccat tcttgaggcc     1920

aacctcctgc cgccgccgga accgaaagcc agctggtgcc gggtaatgga cgaacttgcc     1980

gtcgcttctt gcgaaatcta tcgcaatgtg gtgcgcggcg ataaagattt tgtgccttac     2040

ttccgcagcg ccacaccgga acaggaactg gcaaaactgc ctttaggttc ccgaccggca     2100

aaacgcaatc cgaacggcgg cgttgaaagc ctgcgtgcca ttccctggat cttcgcctgg     2160

atgcaaaacc gcctgatgct gcccgcctgg ctcggtgccg gcgcctcaat ccgtcaggcg     2220

atggaaagcg gcaaagcggc ggtgattgaa gaaatgtgca accattggcc gtttttcaat     2280

acccgaatcg gcatgcttga aatggtattc agtaaaaccg atagctggct gtccgaatat     2340

tacgaccagc gtttagtgaa aaaagagctt tggtatttag gcgaatcgct gcgcaaacag     2400

ttaagcgaag atatcgctac cgtgttacgg ctttccggca aaggcgatca attaatgtcg     2460

gatttgcctt gggtggcgga atctattgca ctgcgtaacg tttacaccga cccgttaaac     2520

ttattgcaag tggaattatt gcgtcgtttg cgagcggatc ccgaacatcc gaatccggat     2580

atcgagcaag cgctgatgat caccattacc ggtatcgccg cgggtatgcg taatacgggt     2640

tag                                                                   2643


<210>  51
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  51
acggcagtat accctatcag g                                                 21


<210>  52
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  52
aatgatccat ggtccgagag                                                   20


<210>  53
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  53
gaagagacgt acaagatccg cc                                                22


<210>  54
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  54
ggataaaagt attacatacg tacaggattg tgtattagtg tatttcg                     47


<210>  55
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  55
taggaatggt gcatcatcca ac                                                22


<210>  56
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  56
ccaaccaaac acgcgtacaa tgaacgaaca atattccgca ttgc                        44


<210>  57
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  57
ggacacggag aacccattta ttc                                               23


<210>  58
<211>  1029
<212>  DNA
<213>  Issatchenkia orientalis

<400>  58
atgtccaatg ttaaagtagc tctactaggt gccgctggtg gtatcggcca accacttgct       60

ctattactta agcttaatcc aaacataacc catttggcac tctatgacgt tgtgcatgtt      120

cctggagtgg ctgccgacct acaccatata gacacagatg tagtgattac ccaccatttg      180

aaagatgaag acggtacggc cttggcaaac gccctcaagg acgctacgtt tgttattgtc      240

cccgccggtg ttccgagaaa gcccggcatg actagaggtg atttgttcac aattaatgcc      300

ggtatatgtg ccgaattggc taatgctatt agtttgaacg ctcctaatgc attcaccctt      360

gtcattacca atccggtcaa ctcgaccgtt cctatattta aggaaatatt tgctaaaaat      420

gaagccttca atccaaggag actgtttggt gtaactgctc tagatcatgt tagatcaaat      480

acttttctct cggaattaat tgacggtaaa aatccccaac attttgatgt cactgttgtt      540

ggcggacact ctggtaactc aattgtcccc ctattctccc ttgttaaggc tgccgaaaat      600

ttagacgatg aaattataga tgccttgatt catagagttc aatacggtgg agatgaagtt      660

gtggaagcaa agagcggtgc gggctcggca actctttcaa tggcttatgc cgctaacaag      720

ttcttcaata tattgcttaa tggatacttg ggtttgaaga agacaatgat ttcaagttat      780

gtctttttag acgattcaat caacggcgtc cctcaattaa aggaaaattt gtctaaactt      840

ttgaaaggtt ccgaggttga gttaccaagt tatttggctg ttccaatgac ctatggtaaa      900

gaaggtattg aacaagtctt ttacgattgg gtgtttgaaa tgtcaccaaa ggaaaaggaa      960

aacttcatta cagcgattga atacattgat caaaatattg aaaaaggtct gaattttatg     1020

gtacgttaa                                                             1029


<210>  59
<211>  1011
<212>  DNA
<213>  Issatchenkia orientalis

<400>  59
atggtcaagg tgactatttt aggcgctgcc ggtggaattg gacaaccact ctcattgtta       60

ttgagactta atccatggat tgacgaattg gccttgtttg atattgtcaa tacccccggc      120

gtgagttgtg atttgtcgca tattcctgca tcacaggttg ttaatggcta tgctccgaaa      180

tcgaaatcag atacagagac aatcaagact gccttgaaag gtgctgatat tgttgttatt      240

cctgcaggaa ttccacgtaa acctggtatg acaagaaacg atctctttaa aatcaatgcc      300

ggaatcgtta agagtttgat tcatagtgca ggaaccactt gccctgatgc atttatttgt      360

gtcatttcga accctgtcaa ctcgacagtt ccaattgccg ttgaagaact aaagcgtttg      420

aatgttttta atccacataa agttttcggt attaccacat tggacaattt cagattagaa      480

gaatttctga gtggagaact tggtggaatt gtcaaaccaa atgatttata tggtgatgta      540

gttgctatag gtggccattc gggcgactct atagtaccga tcttgaattc gtggaatttg      600

aatttcatca atgatggaga ttcttataac aatttggtca agagggtcca gtttggaggc      660

gatgaggttg tcaaggcaaa ggacgggaaa ggttcggcta cattgtcaat ggctacagct      720

gcatacaggt ttgtcaacaa cctcttggac gccattgtca ataacaagaa agtcaaggaa      780

gtggcctttg tgaaaatcga ccaattgcca actacaaggg ttccttattt tgttgttgat      840

gaaactcagt attttagtct acccattatt ctcggtagac aggggattga gagggtcacg      900

ttcccagaat ctctgacaga gcaagaggtg agaatgacaa agcacgctgt tgctaaagtt      960

aaagttgacg ttaataaagg cttcaatttt gtccatggcc caaaactgta a              1011


<210>  60
<211>  1005
<212>  DNA
<213>  Issatchenkia orientalis

<400>  60
atgttctcca gaatctctgc tagacaattc tcctcctctg ctgcttccgc ttacaaggtc       60

accgttttag gtgctgcagg tggtattggc caaccactat ctcttttgat gaagttgaac      120

cacaaggtca ccaacttatc cttgtacgac ttgagattgg gtgctggtgt tgccactgac      180

ttgtcccaca ttccaaccaa ctccgttgtc aagggctatg gtccagaaaa caatggtttg      240

aaggacgcct tgaccggctc cgatgttgtt cttattccag ctggtgttcc aagaaaacca      300

ggtatgacta gagacgatct cttcaacacc aatgcatcga ttgtcagaga cttggcaaag      360

gctgctgcag accactgtcc aaacgccgtc ttgttgatca tttcaaaccc tgtcaactca      420

actgtcccaa ttgttgctga ggttttgaaa tcaaagggcg tctacaaccc aaagaagttg      480

tttggtgtca ccactttgga cgttttgaga tcctcgagat tcttgagtga agtcgtcaac      540

accgacccaa ccaccgaaac cgtcactgtt gttggtggcc actctggtgt caccattgtt      600

cctttaatct cccaaaccaa acacaaggac ttgccaaagg aaacctacga agcattggtc      660

cacagaatcc aattcggtgg tgatgaggtt gtcaaggcca aggacggtgc aggttccgct      720

accttgtcca tggcccaagc cggtgcaaga atggcctcct ccgtcttgaa gggtttggct      780

ggtgaagttg acattgtcga accaaccttt attgactctc cattgttcaa gtccgaaggt      840

gtcgaattct tctcctccag agtcaccctt ggtccagaag gtgtccaaga agtccaccca      900

ttgggcgtct tatctactgc tgaagaagaa atggttgcta ctgctaagga aaccttgaag      960

aagaacatcc aaaagggtgt cgactttgtc aaggctaacc cataa                     1005


<210>  61
<211>  1071
<212>  DNA
<213>  Zygosaccharomyces rouxii

<400>  61
atgcctcatt ctatcaacgg tgatgttaaa atcgcagtat tgggagctgc aggtggtatt       60

ggacaatcac tttcgctact tttgaagacc cagttaacta gagaattgcc aaatcatcgt      120

catgctcagt tagccctata cgacgtcaat gctgacgcag ttcggggtgt cgcagccgac      180

ttatctcata ttgatacagg tgttactgta acaggatatg aaggtgatag gatcggcgaa      240

gcgttagaag gtacggatat cgtcctgatc cctgcaggtg ttcctagaaa acctggtatg      300

acaagagaag atctattggt tgttaatgca aagattgtca agagtatagg gtcatcgatt      360

gcgcagcatt gcgatttaaa caaagtgttc attctactaa tctcaaaccc aataaattcc      420

cttgttccag tactcgttaa ggaactggaa tctaaatctc aaggcactca agttgagaga      480

cgtgtgcttg gtctcactaa gttggattcc gttagagcaa gtgcattttt gcacgaggtt      540

acgattaaac atggtctaaa acctaaatct aatactcttg atgatgttcc agtagttggt      600

ggtcattctg gtgaaactat tgtaccttta ttctcccaag cccctaatgg taaccgttta      660

tcacaggacg ccttggaagc tcttgttcag cgtgtacaat tcggaggcga tgaagtcgtt      720

agagctaaaa atggtgctgg tagtgccact ctgtgtatgg cccatgccgc ttatactgtt      780

gctgcatctt ttattccact tatcactggt caaaagcgtt ccatctctgg tacattctat      840

gttgccttaa aggatgctca aggtcagcct atcaacagta gcgctaagcg tcttttgggc      900

tcaatcaacg atttaccata ttttgcagtg ccattggaga ttacttctca gggtgtggat      960

gaattagata ccagcgtttt ggaaagaatg accaagtatg agagagaaag actcttagct     1020

ccttgtctgg gtaaattgga aggtggtatc agaaacggtt tgagtttgta a              1071


<210>  62
<211>  1017
<212>  DNA
<213>  Kluyveromyces marxianus

<400>  62
atgcttagag ccctaactcg ccgtcaattt tcctccactg ccttcaaccc atacaaggtc       60

accgttctag gtgctggtgg tggtattggt caaccattgt ccttgttgtt gaagctaaac      120

cacaaggtca ctgacttgag actatacgac ttgaagggtg ccaagggtgt cgctgctgac      180

ttgtctcaca tcccaaccaa ctctaccgtt actggttaca ctccagaatc caaggactct      240

caagaagaat tggctgctgc tttgaaggac actgaggttg ttttgatccc agctggtgtg      300

ccaagaaagc caggtatgac ccgtgacgat ttgttcgcca tcaatgccgg tattgtcaga      360

gatttggcca cttccatcgc caagaacgct ccaaacgccg ccatcttggt catctccaac      420

ccagtcaact ctactgtccc aatcgtcgcc gaggtcttga agcaaaacgg cgtctacaac      480

ccaaagaagt tgttcggtgt caccactttg gacgttatcc gtgcctccag attcatctcc      540

gaggttagag gtaccgaccc aaccactgag cacgtgaccg tcgtcggtgg tcactccggt      600

atcaccatct tgccgctagt gtcccagacc aagcacaagt ccgtcatcaa gggcgaggaa      660

ttggacaact tgatccacag aatccaattc ggtggtgacg aagtcgtcca ggcaaagaac      720

ggtgctggtt ctgccacttt gtccatggcc caagccggtg cccgtttcgc taacagcgtt      780

ctaagcggtt tcgaaggtga aagagacgtc attgagccaa ctttcgtcga ctccccattg      840

ttcaaggacg aaggtatcga attcttcgct tccccagtca ctttgggccc agaaggtgtc      900

gaaaagatcc acggtttggg tgtcttgtcc gacaaggaag aacaaatgtt ggccacttgt      960

aaggaaacct tgaagaagaa catcgaaaag ggtcaaaact ttgtcaagca aaactaa        1017


<210>  63
<211>  1017
<212>  DNA
<213>  Kluyveromyces marxianus

<400>  63
atggttagcg ttgcagtatt aggatcatcc ggaggcattg gccaaccact ctcactcttg       60

ttgaagctgg accctcgcgt gtccagcttg agattgtacg acttgaagat gtcccacggg      120

atcgccaccg atttgtcgca catggactcc aactccatct gcgagggctt caacaccgac      180

gagatcgcgc tcgcgctcaa gggcgcccag atcgtcgtca tccccgcggg tgtcccaaga      240

aagcccggga tgtcacgtga cgaccttttc aagatcaacg ccaagatcat caagtcgttg      300

gcgttgcaaa tagccgagca cgcgcccgag gcgcgcgtcc tcgtgatctc gaacccggtc      360

aactccttgg tgcccattgt gtacgagact ttgaagagcg tcggcaagtt cgagccgggt      420

aaagtgatgg gaattaccac attggacatt atccgctcac acacgttcct ggtggacgtc      480

ttgggccgca aggcgtacag cgtcgagaag ttgcgcagcg cggttactgt ggtgggcggc      540

cactcgggcg agaccattgt tccgattttc accgaccaga agttctacag gcgtctcaga      600

gacagagagc tctatgacgc gtacgtgcat agggtccaat tcggcggaga cgaggtcgtg      660

aaggccaagg acggcagcgg tagtgctact ttgtctatgg cctgggcggg ttacagtttt      720

gtgaagcagt tgctcaacag cttgcaccta gaaacaggcg aagacgtgca tccgatccca      780

acgtttgtgt acttgccggg tttaccgggc gggaaggagc tccagcagaa gttgggcacc      840

tctgttgagt tttttgccgc gcccgtgaag ctttccaagg gtattgtggt tgaagttgag      900

cacgactggg tcgacaagtt gaacgatgcc gagaagaagt tgattgcaaa gtgtcttcca      960

atccttgaca agaacatcaa gaagggtctc gccttttcgc agcagacaaa gttgtga        1017


<210>  64
<211>  1125
<212>  DNA
<213>  Kluyveromyces marxianus

<400>  64
atgccagcag tatcatatga tgtccagcaa cgggatatcc tcaagatcgc agttctaggg       60

gcggcaggcg gtattggcca atccttgtcg ctcttgttga agtcgaacgc ttcttttttg      120

ttaccacgtg actcgtcaag acacataagc ctagcgctat acgacgtgaa caaagatgcc      180

atcgtgggca cagcagcaga cttgtcacac atagacaccc ctatcaccac cactccacac      240

tacccaaatg atgggaatgg cggtatcgca cggtgcttgc aagatgcaga catggtcatc      300

atcccagcag gtgtgcccag aaaacccggt atgtcacgtg atgacctaat cggtgtcaac      360

gccaagatca tcaagtcgct aggaaacgac atcgcagagt actgtgactt gtctaaagtg      420

catgtattgg ttatttcgaa cccagtgaac tcgttggtcc cactgatggt gtcgactttg      480

gcaaacagcc cacacagtgc gaacacaaac atcgaggcta gagtgtacgg gatcacccat      540

ttggacctag tgagagcttc cacctttgtg caacagctaa actctttcaa atcaaataac      600

gcacctgaca ttccggtcat tggtggtcat tccggagata ccatcatccc cgttttttcc      660

gtcttgaatc accgcgcttc taactccgga tacgctaatt tgctagataa tggcgttagg      720

caaaagttgg tccacagagt tcaatatggt ggggacgaaa tcgtccaagc aaagaacggt      780

aacgggagcg cgacattatc catggcatac gcgggcttca aaatcgcagc acaattcatc      840

gaccttttgg tcggaaatat ccgcactatc gaaaatattt gcatgtatgt tccgctcact      900

aacaggtata ataccgagat cgccccaggc tctgacgaat taagatcaaa gtacatcaac      960

ggaacccttt atttctcgat tccactttcc atcggaataa acggtatcga aagagtccac     1020

tacgagatca tggaacatct agacagctac gagcgtgaga cgctactacc gatctgcttg     1080

gaaactctaa agggtaatat tgacaagggt ctaagcttgg tataa                     1125


<210>  65
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  65
ggaggaatgg aacagtgatg ac                                                22


<210>  66
<211>  939
<212>  DNA
<213>  Escherichia coli

<400>  66
atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc aggcgcttgc actactgtta       60

aaaacccaac tgccttcagg ttcagaactc tctctgtatg atatcgctcc agtgactccc      120

ggtgtggctg tcgatctgag ccatatccct actgctgtga aaatcaaagg tttttctggt      180

gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc ttatctctgc aggcgtagcg      240

cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta acgccggcat cgtgaaaaac      300

ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca ttggtattat cactaacccg      360

gttaacacca cagttgcaat tgctgctgaa gtgctgaaaa aagccggtgt ttatgacaaa      420

aacaaactgt tcggcgttac cacgctggat atcattcgtt ccaacacctt tgttgcggaa      480

ctgaaaggca aacagccagg cgaagttgaa gtgccggtta ttggcggtca ctctggtgtt      540

accattctgc cgctgctgtc acaggttcct ggcgttagtt ttaccgagca ggaagtggct      600

gatctgacca aacgcatcca gaacgcgggt actgaagtgg ttgaagcgaa ggccggtggc      660

gggtctgcaa ccctgtctat gggccaggca gctgcacgtt ttggtctgtc tctggttcgt      720

gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg ttgaaggcga cggtcagtac      780

gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg gcgtggaaga gcgtaaatct      840

atcggtaccc tgagcgcatt tgaacagaac gcgctggaag gtatgctgga tacgctgaag      900

aaagatatcg ccctgggcga agagttcgtt aataagtaa                             939


<210>  67
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  67
cacagaggtg cagtaacgag                                                   20


<210>  68
<211>  1014
<212>  DNA
<213>  Rhizopus delemar

<400>  68
atgtttgccg cctctcgtgt tttctctatt gctgccaagc gttctttctc tacttctgct       60

gctaatcttt ccaaggttgc cgttcttggc gctgctggtg gtattggtca acccttgtct      120

ttgttgttga aggaaaaccc tcacgtcacc cacctttctc tttatgatat tgtcaacact      180

cctggtgtcg ctgccgatct tagccacatc aacaccaact ccaaggtcac tggccacacc      240

cctgaaaacg atggtttgaa gactgctctt gaaggtgctc acgttgttgt tattcctgct      300

ggcgttcctc gtaagcctgg tatgacccgt gatgatttat tcaacaccaa tgcttccatt      360

gttcgtgacc ttgctgaagc tgctgccaag cactgtcccg acgctcattt ccttatcatc      420

tccaaccctg tcaactccac tgttcccatc tttgccgaaa ccttaaagaa ggctggtgtc      480

ttcaacccta agcgtttgta tggtgtcacc actcttgatg tcgtccgtgc ctctcgcttc      540

gttgccgaag tcaagaactt ggaccccaac gatgtcaagg ttaccgttgt cggtggtcac      600

tctggtgtga ctattgtccc tctcctctct caaaccggtc tcgaattcag caaggaagaa      660

ctcgatgcct tgacccaccg tatccaattc ggtggtgatg aagtcgttca agccaagaat      720

ggtactggtt ctgtcactct ctccatggcc tttgccggtg ctcgtttcgc caactctgtc      780

ttggaagcca ctgttggtgg taagaagggt gttgttgaac cctcctttgt caagtctgat      840

gtctttgcca aggatggtgt tgaatatttc tctaccaaca ttgaacttgg tcctgaaggt      900

gttgaaaaga tcaacgaact cggtcaaatc tctgactatg aaaaggaact tattgctaag      960

gccgttcctg aattaaagaa gaacattgcc aagggtaaca gctttgttca ataa           1014


<210>  69
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  69
caagagtatc ccatctgaca ggaaccgatg g                                      31


<210>  70
<211>  1458
<212>  DNA
<213>  Issatchenkia orientalis

<400>  70
atgttagctg ctagatcatt aaaggcaaga atgtcaacaa gagctttctc aactacctca       60

attgcaaaaa gaatcgaaaa agatgcattt ggtgacattg aagtcccaaa tgagaaatat      120

tggggtgctc aaactcaaag atctttacaa aatttcaaaa ttggtggtaa gagagaagtt      180

atgccagaac caatcatcaa atcttttggt attttaaaga aggctactgc taagatcaat      240

gctgagtctg gtgctttaga cccaaagtta tctgaagcca tccaacaagc tgcaaccgaa      300

gtttatgaag gtaaactaat ggaccatttc ccattagttg tctttcaaac cggttctggt      360

actcaatcta acatgaatgc caatgaagtc atctctaata gagcaattga aatcttgggt      420

ggtgaattag gctctaaaac tccagtccat cctaatgatc atgttaatat gtcccaatct      480

tctaatgata ctttccctac tgtcatgcat attgcagcag ttacagaagt ttcatcccat      540

ttattaccag aattaactgc actaagagat gcattgcaaa agaaatccga tgaatttaag      600

aatattatca aaatcggtag aacccattta caagatgcaa ctcctttaac tttaggtcaa      660

gaattttctg gttatgttca acaatgtact aatggtatca aaagaatcga aattgctctt      720

gaacatttga gatacttagc tcaaggtggt actgccgttg gtactggtct taacaccaag      780

aaaggttttg ctgaaaaggt tgcaaatgaa gtcactaaat tgactggttt acaattctat      840

accgctccaa ataaattcga agcccttgca gctcacgatg ctgttgttga aatgtctggt      900

gctttgaata ccgttgcagt ctcattattc aaaatcgctc aagatatcag atatttgggt      960

tccggcccaa gatgtggtta tggtgaattg gctttaccag aaaatgaacc aggttcttcc     1020

atcatgccgg gtaaagttaa cccaactcaa aacgaagctt tgactatgct ttgtacccaa     1080

gtctttggta accactcttg tattaccttt gcaggtgctt caggtcaatt cgaattgaat     1140

gtctttaagc cagttatgat ctccaacttg ttatcttcta ttaggttatt aggtgatggt     1200

tgtaattctt ttagaatcca ctgtgttgaa ggtatcattg caaataccga caagattgat     1260

aaattactac atgaatctct catgttagtt actgctttga acccacacat tggttacgat     1320

aaggcttcca agattgcaaa gaatgcacac aagaagggct tgacattgaa acaatctgca     1380

ttggaattag gttacttgac cgaagaacaa ttcaatgaat gggttagacc agaaaacatg     1440

attggtccaa aggattaa                                                   1458


<210>  71
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  71
catcactgtt aaaggaatgg gtaaatc                                           27


<210>  72
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  72
gctggagaat agatcttcaa cgccccg                                           27


<210>  73
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  73
gagaacttat acgcaccaga acgccttttg                                        30


<210>  74
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  74
caagagtatc ccatctgaca ggaaccgatg g                                      31


<210>  75
<211>  1413
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  75
atgtctctct ctcccgttgt tgttattgga accggtttgg ccgggctggc tgctgccaac       60

gaattggtta acaagtataa catccctgta accatcctcg aaaaggcttc ctcgatcggt      120

gggaactcta tcaaggcctc cagtggtatt aacggtgctt gcaccgagac tcaacgtcac      180

ttccacatcg aggactcccc acgcttattt gaagatgaca ccatcaagtc tgctaaaggt      240

aaaggtgtcc aagagttaat ggctaagttg gccaatgatt ctcccctggc tattgaatgg      300

ttgaaaaacg aatttgattt gaaattggac ctattggctc aattgggtgg ccactctgtg      360

gcaagaactc acagatcgtc tgggaagttg cctccaggtt tcgaaattgt ttctgcctta      420

tctaacaatt tgaagaaatt agctgagact aaaccagagt tagttaagat taacttagac      480

agtaaagtcg tagacatcca tgaaaaggat ggctccattt ctgctgtagt gtacgaggat      540

aagaatggcg aaaagcacat ggtgagtgct aacgatgtcg ttttttgttc tggagggttt      600

ggcttttcta aggaaatgct taaagaatat gcacccgaac tggtgaactt gccaacgaca      660

aacgggcaac aaacaactgg tgatggtcaa aggcttctgc agaagttagg cgctgatctg      720

attgacatgg accaaattca agttcatcca actgggttca ttgatccaaa tgaccgtagc      780

tcaagctgga aattcttggc tgccgaatcc ttaagaggtc ttggtggtat cctattaaac      840

cctattaccg gtagaagatt tgtcaacgaa ttgaccacaa gagatgtagt cactgcagct      900

attcaaaagg tttgtcctca agaggataac agagcactat tggttatggg cgaaaaaatg      960

tacacagatt tgaagaataa tttagatttt tacatgttca agaaacttgt acagaaattg     1020

acattatctc aagttgtgtc tgaatataat ttaccaatca ctgtcaccca attatgcgag     1080

gaattgcaaa catactcttc gttcactacc aaggctgatc cgttgggacg taccgttatt     1140

ctcaacgaat ttggctctga cgttactcca gaaaccgtgg tttttattgg tgaagtaaca     1200

ccggttgtcc atttcaccat gggtggtgct agaatcaatg tcaaggctca agtcattggc     1260

aagaacgacg aaaggctact aaaaggcctg tacgcggccg gtgaagtttc tggcggtgtt     1320

catggcgcca ataggttggg tggttcaagt ttgttagaat gcgttgtctt tgggagaact     1380

gcagctgaat ctattgccaa tgaccgcaag taa                                  1413


<210>  76
<211>  1413
<212>  DNA
<213>  Saccharomyces mikatae

<400>  76
atgtcatctt ctccagttgt cgttattggt acaggcttgg caggtttggc aactgctaat       60

gagttagtca ataagtacaa cattcctgtt accattttgg aaaaggcatc ctctatcggt      120

ggcaattcca ttaaggcatc ttctggtatc aatggtgcat gtacagaaac ccaacgtcat      180

tttcacattg aagatactcc tagacttttt gaagatgata ctgttcaatc cgccaagggc      240

aaaggtgttc aagagttaat gggtaaactt gctaatgatt ctccacttgc tattgaatgg      300

ttaaagactg aattcgactt aaagttagac cttttggctc agttaggtgg tcactctgtt      360

gctagaactc atagatcttc cggtaaactt ccaccaggtt tcgaaatcgt ttccgcctta      420

tccaataact tgaaaaagtt ggcagaaacc aagccagagt tagttaagat taacttagac      480

tcaaaggtcg ttgacatcca caaaaaggac ggctctattt ccgcaattgt ctatgatgac      540

aaaaacggtg aaagacatac cttatccact tcaaatgttg ttttctgctc tggtggtttc      600

ggtttttcta aggaaatgtt aaacgagtat gctccacaat tggtcaactt gccaaccact      660

aacggtcagc aaacaacagg tgacggccaa agattgttac aaaagcttgg tgcagatttg      720

attgatatgg atcaaattca agtccatcct actggtttca tcgacccaaa cgatagaaac      780

tcctcttgga agtttttggc tgctgaatct ttaagaggtt tgggtggtat cttattgaat      840

ccaattactg gtcgtagatt tgtcaacgaa ttgaccacta gagatgtcgt tactgaagca      900

atccagaagc actgtccaca agatgataac agagctttgt tagttatgtc cgaaaagatg      960

tatacagatt tgaaaaacaa tttggacttc tacatgttca aaaagttagt tcaaaagtta     1020

tctttgtccc aagttgtttc cgagtataag ttaccaatta ctgtttccca attgtgtcag     1080

gaattacaaa cctactcatc ttttacttca aaagccgatc ctcttggtag aaccgttgtc     1140

ttaaacgaat tcggtgctga catcacccca gaaacaatgg ttttcatcgg cgaagttacc     1200

ccagtcgttc actttaccat gggtggtgct agaatcaatg ttaaggctca agttatcggc     1260

aaaaacgatg agcctttgtt aaacggtttg tacgcagcag gtgaagtttc tggtggtgtc     1320

catggtgcca atagattagg tggttcatct ttgcttgaat gtgtcgtttt tggtagaact     1380

gcagcagaat caattgccaa taaccacaag taa                                  1413


<210>  77
<211>  1413
<212>  DNA
<213>  Kluyveromyces polysporus

<400>  77
atgtcaacca aaaagccagt cgtcatcatt ggtactggtt tagccggttt gtctgctggt       60

aatcaattgg tcaatatgca taaagttcct atcattatgt tggacaaggc atcctccatt      120

ggtggtaatt ctacaaaggc ttcctctggt atcaacggtg cttctactat tactcaacag      180

caacttaatg ttaaagactc tcctgactta ttccttcaag atactgttaa gtctgctaag      240

ggtagaggta ttgagtccct tatgaaaaag ttatcacaag actccaactc tgctatccat      300

tggttgcaac aggattttga tttgaagttg gatttgttag ctcaattggg tggtcattcc      360

gttcctagaa cacaccgttc ctcaggcaag ttacctccag gcttcgaaat tgtccaagct      420

ttatctaaca agttaaaggc tatttctgag tccgatccag aattcgttag aatcttactt      480

aactccaagg ttgttgatgt ttccgttaac aatgagggca aggtcgaatc tattgactat      540

gttgatgcag aaggtaaaca tcacaaaatc gctactgata acgttgtctt ttgttccggt      600

ggtttcggtc actcagcaga aatgttgaac aagtatgcac cagaattagc taacttgcca      660

actactaacg gtcaacaaac cactggcgat ggtcagagaa tcttggagaa attgggtgca      720

gacttgattg atatgtccca aattcaagtt cacccaacag gtttcatcga tccagcaaac      780

agagattcta agtggaagtt tttggctgcc gaagcattaa gaggtttagg tggtatctta      840

cttaatccat ctaccggcaa gagattcgtt aatgagttaa ccaccagaga tttggtcaca      900

gaagctatcc aatcacaatg tccaagagat gacaataagg cattccttgt tatgtctgaa      960

aaggtctatg agaattacaa aaacaacatg gacttttact tattcaaaaa gttagtttcc     1020

aagatgacca ttaaggaatt tgtcgaaact tacaagttgc caatttctgc cgacgccgtt     1080

acccaagact taatcgacta ttcagttgat aagaccgata agtttggtag accattggtt     1140

atcaacgttt ttgatgaaaa gttgaccgaa gattccgaaa tctatgttgg tgaagttaca     1200

ccagttgtcc atttcactat gggtggtgca aagatcaata ctgaatctca agttatcaac     1260

aaaaacggtc aagttttggc aaagggtatc tacgcagcag gtgaagtctc cggtggtgtt     1320

cacggttcta atagattagg tggttcatct ttgttagaat gcgtcgttta cggtagatct     1380

gctgcagata acattgccaa aaacattgaa taa                                  1413


<210>  78
<211>  1464
<212>  DNA
<213>  Kluyveromyces marxianus

<400>  78
atgttgcaca gatacatccg tttgttctcc ttctgcgtca tcttgtactt agtctatttg       60

ttacttacta aggagtcaaa cgtcatgtct aagcctgttg ttgttattgg ttctggttta      120

gcaggcttaa caacatcttc acaattagca aagtttaaca ttccaatcgt ccttttagaa      180

aagacatctt ccattggtgg taattccatt aaggcatctt ctggtatcaa tggcgcaggc      240

accgaaactc aatctcgttt acacgttgaa gatcacccag aattgtttgc tgatgatacc      300

attaagtctg caaaaggtaa aggtgttgtc gctttgatgg aaaagttatc taaagactcc      360

tctgatgcta tttcctggtt acaaaacgac ttcaagattc ctttggataa gttagctcaa      420

ttaggcggtc attccgttcc tagaacccat agatcatccg gcaagcttcc accaggtttc      480

caaattgtcg ataccttgaa aaaggccttg gagtcttatg actctaaagc agttaagatc      540

caattgaatt ctaaggtcgt tgatgttaag cttgattcca ataacagagt ttcatctgtt      600

gttttcgaag atcaagatgg tactcacacc attgaaacca acaacgtcgt tttctgtact      660

ggtggtttcg gtttcaacaa aaagttattg gagaagtatg caccacactt ggtcgacttg      720

ccaactacca acggtgagca aaccttaggt gaaggtcagg tcttattgga aaaacttggt      780

gctaagttga ttgatatgga ccaaattcaa gttcatccaa ctggctttat cgatccagcc      840

aatccagatt ctaattggaa gtttttggct gccgaggcct taagaggttt aggtggtgtc      900

ttgatcaatc cacacactgg tcagagattt gttaacgaat tgacaactag agacatggtc      960

accgaagcta tccagtctaa gtccgaatcc aagactgctt acttggttat gtccgagtcc     1020

ttatacgaga actacaagcc aaacatggac ttctatatgt tcaaaaagct tgtttccaaa     1080

aagaccgttg ctgaatttgc tgaagatttg ccagtttctg ttgaccaact tattgcagaa     1140

ctttcaactt attccgactt gtctaaggat gatcatttgg gtagaaagtt tagagaaaac     1200

acttttggtt cctcattatc atcagactca accattttcg ttggcaagat tactcctgtt     1260

gttcacttca caatgggtgg tgcaaagatt gatgaacaag ctagagtctt gaatgcagaa     1320

ggtaaaccat tagctactgg tatctacgcc gctggtgaag tttctggtgg tgtccatggt     1380

gctaatagat taggtggttc ctctttgtta gaatgtgttg tctttggtag acaagcagca     1440

aaatccatta gagcaaactt gtaa                                            1464


<210>  79
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  79
gaggaagttc aaagtatgaa agacgtcag                                         29


<210>  80
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  80
gatcgggccc gtcttggaag acgcactagt ctc                                    33


<210>  81
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  81
tgacaggaac cgatggactc                                                   20


<210>  82
<211>  1144
<212>  PRT
<213>  Leishmania mexicana

<400>  82

Met Ala Asp Gly Lys Thr Ser Ala Ser Val Val Ala Val Asp Ala Glu 
1               5                   10                  15      


Arg Ala Ala Lys Glu Arg Asp Ala Ala Ala Arg Ala Met Leu Gln Gly 
            20                  25                  30          


Gly Gly Val Ser Pro Ala Gly Lys Ala Gln Leu Leu Lys Lys Gly Leu 
        35                  40                  45              


Val His Thr Val Pro Tyr Thr Leu Lys Val Val Val Ala Asp Pro Lys 
    50                  55                  60                  


Glu Met Glu Lys Ala Thr Ala Asp Ala Glu Glu Val Leu Gln Ala Ala 
65                  70                  75                  80  


Phe Gln Val Val Asp Thr Leu Leu Asn Asn Phe Asn Glu Asn Ser Glu 
                85                  90                  95      


Val Ser Arg Val Asn Arg Leu Ala Val Gly Glu Glu His Gln Met Ser 
            100                 105                 110         


Glu Thr Leu Lys His Val Met Ala Cys Cys Gln Lys Val Tyr His Ser 
        115                 120                 125             


Ser Arg Gly Val Phe Asp Pro Ala Val Gly Pro Leu Val Arg Glu Leu 
    130                 135                 140                 


Arg Glu Ala Ala His Lys Gly Lys Thr Val Pro Ala Glu Arg Val Asn 
145                 150                 155                 160 


Asp Leu Leu Ser Lys Cys Thr Leu Asn Ala Ser Phe Ser Ile Asp Met 
                165                 170                 175     


Ser Arg Gly Met Ile Ala Arg Lys His Pro Asp Ala Met Leu Asp Leu 
            180                 185                 190         


Gly Gly Val Asn Lys Gly Tyr Gly Ile Asp Tyr Ile Val Glu His Leu 
        195                 200                 205             


Asn Ser Leu Gly Tyr Asp Asp Val Phe Phe Glu Trp Gly Gly Asp Val 
    210                 215                 220                 


Arg Ala Ser Gly Lys Asn Gln Leu Ser Gln Pro Trp Ala Val Gly Ile 
225                 230                 235                 240 


Val Arg Pro Pro Ala Leu Ala Asp Ile Arg Thr Val Val Pro Glu Asp 
                245                 250                 255     


Lys Arg Ser Phe Ile Arg Val Val Arg Leu Asn Asn Glu Ala Ile Ala 
            260                 265                 270         


Thr Ser Gly Asp Tyr Glu Asn Leu Val Glu Gly Pro Gly Ser Lys Val 
        275                 280                 285             


Tyr Ser Ser Thr Phe Asn Pro Thr Ser Lys Asn Leu Leu Glu Pro Thr 
    290                 295                 300                 


Glu Ala Gly Met Ala Gln Val Ser Val Lys Cys Cys Ser Cys Ile Tyr 
305                 310                 315                 320 


Ala Asp Ala Leu Ala Thr Ala Ala Leu Leu Lys Asn Asp Pro Ala Ala 
                325                 330                 335     


Val Arg Arg Ile Leu Asp Asn Trp Arg Tyr Val Arg Asp Thr Val Thr 
            340                 345                 350         


Asp Tyr Thr Thr Tyr Thr Arg Glu Gly Glu Arg Val Ala Lys Met Leu 
        355                 360                 365             


Glu Ile Ala Thr Glu Asp Ala Glu Met Arg Ala Lys Arg Ile Lys Gly 
    370                 375                 380                 


Ser Leu Pro Ala Arg Val Ile Ile Val Gly Gly Gly Leu Ala Gly Cys 
385                 390                 395                 400 


Ser Ala Ala Ile Glu Ala Ala Asn Cys Gly Ala His Val Ile Leu Leu 
                405                 410                 415     


Glu Lys Glu Pro Lys Leu Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly 
            420                 425                 430         


Ile Asn Ala Trp Gly Thr Arg Ala Gln Ala Lys Gln Gly Val Met Asp 
        435                 440                 445             


Gly Gly Lys Phe Phe Glu Arg Asp Thr His Arg Ser Gly Lys Gly Gly 
    450                 455                 460                 


Asn Cys Asp Pro Cys Leu Val Lys Thr Leu Ser Val Lys Ser Ser Asp 
465                 470                 475                 480 


Ala Val Lys Trp Leu Ser Glu Leu Gly Val Pro Leu Thr Val Leu Ser 
                485                 490                 495     


Gln Leu Gly Gly Ala Ser Arg Lys Arg Cys His Arg Ala Pro Asp Lys 
            500                 505                 510         


Ser Asp Gly Thr Pro Val Pro Val Gly Phe Thr Ile Met Lys Thr Leu 
        515                 520                 525             


Glu Asn His Ile Val Asn Asp Leu Ser Arg His Val Thr Val Met Thr 
    530                 535                 540                 


Gly Ile Thr Val Thr Ala Leu Glu Ser Thr Ser Arg Val Arg Pro Asp 
545                 550                 555                 560 


Gly Val Leu Val Lys His Val Thr Gly Val His Leu Ile Gln Ala Ser 
                565                 570                 575     


Gly Gln Ser Met Val Leu Asn Ala Asp Ala Val Ile Leu Ala Thr Gly 
            580                 585                 590         


Gly Phe Ser Asn Asp His Thr Pro Asn Ser Leu Leu Gln Gln Tyr Ala 
        595                 600                 605             


Pro Gln Leu Ser Ser Phe Pro Thr Thr Asn Gly Val Trp Ala Thr Gly 
    610                 615                 620                 


Asp Gly Val Lys Met Ala Ser Lys Leu Gly Val Ala Leu Val Asp Met 
625                 630                 635                 640 


Asp Lys Val Gln Leu His Pro Thr Gly Leu Leu Asp Pro Lys Asp Pro 
                645                 650                 655     


Ser Asn Arg Thr Lys Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly 
            660                 665                 670         


Gly Val Leu Leu Asn Lys Asn Gly Glu Arg Phe Val Asn Glu Leu Asp 
        675                 680                 685             


Leu Arg Ser Val Val Ser Gln Ala Ile Ile Ala Gln Asp Asn Glu Tyr 
    690                 695                 700                 


Pro Gly Ser Gly Gly Ser Lys Phe Ala Tyr Cys Val Leu Asn Glu Thr 
705                 710                 715                 720 


Ala Ala Lys Leu Phe Gly Lys Asn Phe Leu Gly Phe Tyr Trp Asn Arg 
                725                 730                 735     


Leu Gly Leu Phe Gln Lys Val Asp Ser Val Ala Gly Leu Ala Lys Leu 
            740                 745                 750         


Ile Gly Cys Pro Glu Ala Asn Val Val Ala Thr Leu Lys Gln Tyr Glu 
        755                 760                 765             


Glu Leu Ser Ser Lys Lys Leu Asn Pro Cys Pro Leu Thr Gly Lys Ser 
    770                 775                 780                 


Val Phe Pro Cys Val Leu Gly Thr Gln Gly Pro Tyr Tyr Val Ala Leu 
785                 790                 795                 800 


Val Thr Pro Ser Ile His Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro 
                805                 810                 815     


Ser Ala Glu Met Gln Thr Ile Asp Asn Ser Gly Val Thr Pro Val Arg 
            820                 825                 830         


Arg Pro Ile Leu Gly Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val 
        835                 840                 845             


His Gly Gly Asn Arg Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val 
    850                 855                 860                 


Phe Gly Lys Ile Ala Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys 
865                 870                 875                 880 


Asn Thr Gly Leu Ser Met Thr Glu Trp Ser Thr Val Val Leu Arg Glu 
                885                 890                 895     


Val Arg Glu Gly Gly Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe 
            900                 905                 910         


Asn Met Pro Gly Ala Leu Gln Arg Thr Gly Leu Ala Leu Gly Gln Phe 
        915                 920                 925             


Ile Gly Ile Arg Gly Asp Trp Asp Gly His Arg Leu Ile Gly Tyr Tyr 
    930                 935                 940                 


Ser Pro Ile Thr Leu Pro Asp Asp Val Gly Val Ile Gly Ile Leu Ala 
945                 950                 955                 960 


Arg Ala Asp Lys Gly Arg Leu Ala Glu Trp Ile Ser Ala Leu Gln Pro 
                965                 970                 975     


Gly Asp Ala Val Glu Met Lys Ala Cys Gly Gly Leu Ile Ile Asp Arg 
            980                 985                 990         


Arg Phe Ala Glu Arg His Phe Phe  Phe Arg Gly His Lys  Ile Arg Lys 
        995                 1000                 1005             


Leu Ala  Leu Ile Gly Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln 
    1010                 1015                 1020             


Ile Val  Arg Ala Ala Val Lys  Lys Pro Phe Val Asp  Ser Ile Glu 
    1025                 1030                 1035             


Ser Ile  Gln Phe Ile Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr 
    1040                 1045                 1050             


Tyr Arg  Thr Leu Leu Glu Ser  Tyr Glu Glu Glu Tyr  Gly Ser Glu 
    1055                 1060                 1065             


Lys Phe  Lys Cys His Phe Val  Leu Asn Asn Pro Pro  Ala Gln Trp 
    1070                 1075                 1080             


Thr Asp  Gly Val Gly Phe Val  Asp Thr Ala Leu Leu  Arg Ser Ala 
    1085                 1090                 1095             


Val Gln  Ala Pro Ser Asn Asp  Leu Leu Val Ala Ile  Cys Gly Pro 
    1100                 1105                 1110             


Pro Ile  Met Gln Arg Ala Val  Lys Gly Ala Leu Lys  Gly Leu Gly 
    1115                 1120                 1125             


Tyr Asn  Met Asn Leu Val Arg  Thr Val Asp Glu Thr  Glu Pro Pro 
    1130                 1135                 1140             


Ser 
    


<210>  83
<211>  743
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  83

Met Asp Gly Pro Asn Phe Ala His Gln Gly Gly Arg Ser Gln Arg Thr 
1               5                   10                  15      


Thr Glu Leu Tyr Ser Cys Ala Arg Cys Arg Lys Leu Lys Lys Lys Cys 
            20                  25                  30          


Gly Lys Gln Ile Pro Thr Cys Ala Asn Cys Asp Lys Asn Gly Ala His 
        35                  40                  45              


Cys Ser Tyr Pro Gly Arg Ala Pro Arg Arg Thr Lys Lys Glu Leu Ala 
    50                  55                  60                  


Asp Ala Met Leu Arg Gly Glu Tyr Val Pro Val Lys Arg Asn Lys Lys 
65                  70                  75                  80  


Val Gly Lys Ser Pro Leu Ser Thr Lys Ser Met Pro Asn Ser Ser Ser 
                85                  90                  95      


Pro Leu Ser Ala Asn Gly Ala Ile Thr Pro Gly Phe Ser Pro Tyr Glu 
            100                 105                 110         


Asn Asp Asp Ala His Lys Met Lys Gln Leu Lys Pro Ser Asp Pro Ile 
        115                 120                 125             


Asn Leu Val Met Gly Ala Ser Pro Asn Ser Ser Glu Gly Val Ser Ser 
    130                 135                 140                 


Leu Ile Ser Val Leu Thr Ser Leu Asn Asp Asn Ser Asn Pro Ser Ser 
145                 150                 155                 160 


His Leu Ser Ser Asn Glu Asn Ser Met Ile Pro Ser Arg Ser Leu Pro 
                165                 170                 175     


Ala Ser Val Gln Gln Ser Ser Thr Thr Ser Ser Phe Gly Gly Tyr Asn 
            180                 185                 190         


Thr Pro Ser Pro Leu Ile Ser Ser His Val Pro Ala Asn Ala Gln Ala 
        195                 200                 205             


Val Pro Leu Gln Asn Asn Asn Arg Asn Thr Ser Asn Gly Asp Asn Gly 
    210                 215                 220                 


Ser Asn Val Asn His Asp Asn Asn Asn Gly Ser Thr Asn Thr Pro Gln 
225                 230                 235                 240 


Leu Ser Leu Thr Pro Tyr Ala Asn Asn Ser Ala Pro Asn Gly Lys Phe 
                245                 250                 255     


Asp Ser Val Pro Val Asp Ala Ser Ser Ile Glu Phe Glu Thr Met Ser 
            260                 265                 270         


Cys Cys Phe Lys Gly Gly Arg Thr Thr Ser Trp Val Arg Glu Asp Gly 
        275                 280                 285             


Ser Phe Lys Ser Ile Asp Arg Ser Leu Leu Asp Arg Phe Ile Ala Ala 
    290                 295                 300                 


Tyr Phe Lys His Asn His Arg Leu Phe Pro Met Ile Asp Lys Ile Ala 
305                 310                 315                 320 


Phe Leu Asn Asp Ala Ala Thr Ile Thr Asp Phe Glu Arg Leu Tyr Asp 
                325                 330                 335     


Asn Lys Asn Tyr Pro Asp Ser Phe Val Phe Lys Val Tyr Met Ile Met 
            340                 345                 350         


Ala Ile Gly Cys Thr Thr Leu Gln Arg Ala Gly Met Val Ser Gln Asp 
        355                 360                 365             


Glu Glu Cys Leu Ser Glu His Leu Ala Phe Leu Ala Met Lys Lys Phe 
    370                 375                 380                 


Arg Ser Val Ile Ile Leu Gln Asp Ile Glu Thr Val Arg Cys Leu Leu 
385                 390                 395                 400 


Leu Leu Gly Ile Tyr Ser Phe Phe Glu Pro Lys Gly Ser Ser Ser Trp 
                405                 410                 415     


Thr Ile Ser Gly Ile Ile Met Arg Leu Thr Ile Gly Leu Gly Leu Asn 
            420                 425                 430         


Arg Glu Leu Thr Ala Lys Lys Leu Lys Ser Met Ser Ala Leu Glu Ala 
        435                 440                 445             


Glu Ala Arg Tyr Arg Val Phe Trp Ser Ala Tyr Cys Phe Glu Arg Leu 
    450                 455                 460                 


Val Cys Thr Ser Leu Gly Arg Ile Ser Gly Ile Asp Asp Glu Asp Ile 
465                 470                 475                 480 


Thr Val Pro Leu Pro Arg Ala Leu Tyr Val Asp Glu Arg Asp Asp Leu 
                485                 490                 495     


Glu Met Thr Lys Leu Met Ile Ser Leu Arg Lys Met Gly Gly Arg Ile 
            500                 505                 510         


Tyr Lys Gln Val His Ser Val Ser Ala Gly Arg Gln Lys Leu Thr Ile 
        515                 520                 525             


Glu Gln Lys Gln Glu Ile Ile Ser Gly Leu Arg Lys Glu Leu Asp Glu 
    530                 535                 540                 


Ile Tyr Ser Arg Glu Ser Glu Arg Arg Lys Leu Lys Lys Ser Gln Met 
545                 550                 555                 560 


Asp Gln Val Glu Arg Glu Asn Asn Ser Thr Thr Asn Val Ile Ser Phe 
                565                 570                 575     


His Ser Ser Glu Ile Trp Leu Ala Met Arg Tyr Ser Gln Leu Gln Ile 
            580                 585                 590         


Leu Leu Tyr Arg Pro Ser Ala Leu Met Pro Lys Pro Pro Ile Asp Ser 
        595                 600                 605             


Leu Ser Thr Leu Gly Glu Phe Cys Leu Gln Ala Trp Lys His Thr Tyr 
    610                 615                 620                 


Thr Leu Tyr Lys Lys Arg Leu Leu Pro Leu Asn Trp Ile Thr Leu Phe 
625                 630                 635                 640 


Arg Thr Leu Thr Ile Cys Asn Thr Ile Leu Tyr Cys Leu Cys Gln Trp 
                645                 650                 655     


Ser Ile Asp Leu Ile Glu Ser Lys Ile Glu Ile Gln Gln Cys Val Glu 
            660                 665                 670         


Ile Leu Arg His Phe Gly Glu Arg Trp Ile Phe Ala Met Arg Cys Ala 
        675                 680                 685             


Asp Val Phe Gln Asn Ile Ser Asn Thr Ile Leu Asp Ile Ser Leu Ser 
    690                 695                 700                 


His Gly Lys Val Pro Asn Met Asp Gln Leu Thr Arg Glu Leu Phe Gly 
705                 710                 715                 720 


Ala Ser Asp Ser Tyr Gln Asp Ile Leu Asp Glu Asn Asn Val Asp Val 
                725                 730                 735     


Ser Trp Val Asp Lys Leu Val 
            740             


<210>  84
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  84
caatccaacc gccaccg                                                      17


<210>  85
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  85
ggataaaagt attacatacg tacaggattg tgtattagtg tatttcg                     47


<210>  86
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  86
ctttcattag gttggttgaa g                                                 21


<210>  87
<211>  1521
<212>  DNA
<213>  Issatchenkia orientalis

<400>  87
atgggtgtcc agtttatcga aaataccatt atcgttgtct ttggtgcgtc tggagattta       60

gccaagaaga agactttccc cgccctgttt ggactattca gggagggcca gctctcagaa      120

acaaccaaaa tcattgggtt tgctcgatca aaactatcaa atgatgactt gaggaacaga      180

ataaagccgt acttgaaatt gaacaagaga acagatgctg aaaggcagtc tctggagaag      240

tttctgcaga ttctcgagta tcaccagtca aactacgacg acagtgaagg ttttgaaaaa      300

ttggagaagc taatcaataa gtacgacgat gaggcaaacg tgaaagagtc tcacaggttg      360

tactatttgg ctttaccacc gtctgtcttt acaaccgttg caacaatgtt gaaaaaacat      420

tgtcatccag gtgattctgg tattgctagg ctaattgtcg agaaaccctt tggccatgac      480

ttgagctcgt cccgtgagct acaaaagtct ttagctccac tttggaatga agatgaattg      540

tttagaattg atcattattt gggcaaagaa atggttaaga atttaattcc tttgaggttt      600

tcaaatacgt ttttgagcag ttcttggaac aatcaattta ttgacaccat ccaaatcact      660

tttaaggaga actttggaac tgaaggacgt ggtggttact ttgattccat tggtataata      720

agagatgtta tccaaaatca tttgttacaa gtcttgacta ttgttttgat ggaaaaacca      780

gcggatttta atggagaatc tatcagagat gaaaaggtta aagtgttaaa ggcaattgaa      840

caaattgatt tcaataatgt gttggtaggt caatatgata aatctgaaga tggtagtaaa      900

cctggttact tggatgatga taccgtcaat ccagattcta aagctgtcac ttatgctgcc      960

ttagttttaa atgtggcaaa cgaaagatgg aataatgttc cgatcattct aaaggcaggc     1020

aaggccttga atcaatccaa ggtggaaatt agaatccagt tcaaaccagt agaaaatgga     1080

atcttcaaaa actctgctag gaatgagttg gttattagga tccaaccaaa cgaggcaatg     1140

tatttgaaaa tgaacatcaa agtacctggt gtttccaatc aagtgtcgat ttcagaaatg     1200

gatttgactt acaagaatag gtattcctcc gaattttaca ttccagaagc ttatgaatct     1260

ttgattaaag atgccttaat ggatgatcat tcaaattttg ttagagacga tgaattggac     1320

atttcatggg ctttgttcac tccattacta gaacatatcg aaggccccga tggtccaact     1380

ccaaccaagt atccttacgg ttccagaggt ccaaaggaga ttgacgaatt tttgagaaac     1440

catggttatg taaaggaacc aagagaaaat taccaatggc cattaactac tcctaaagaa     1500

ttgaacagtt caaagtttta a                                               1521


<210>  88
<211>  477
<212>  PRT
<213>  Issatchenkia orientalis

<400>  88

Met Gly Gln Asn Leu Ile Leu Asn Ala Ala Asp His Gly Phe Thr Val 
1               5                   10                  15      


Val Ala Tyr Asn Arg Thr Val Ser Lys Val Asp His Phe Leu Gln Asn 
            20                  25                  30          


Glu Ala Lys Gly Lys Ser Ile Ile Gly Ala His Ser Ile Glu Glu Leu 
        35                  40                  45              


Cys Ala Lys Leu Lys Lys Pro Arg Arg Ile Met Leu Leu Val Lys Ala 
    50                  55                  60                  


Gly Asn Pro Val Asp Gln Phe Ile Glu Gln Leu Leu Pro His Leu Asp 
65                  70                  75                  80  


Glu Gly Asp Ile Ile Ile Asp Gly Gly Asn Ser His Phe Pro Asp Ser 
                85                  90                  95      


Asn Arg Arg Tyr Glu Glu Leu Lys Lys Lys Gly Ile Leu Phe Val Gly 
            100                 105                 110         


Ser Gly Val Ser Gly Gly Glu Glu Gly Ala Arg Tyr Gly Pro Ser Leu 
        115                 120                 125             


Met Pro Gly Gly Ala Lys Glu Ala Trp Pro His Ile Lys Asp Ile Phe 
    130                 135                 140                 


Gln Ser Ile Ser Ala Lys Ala Asp Gly Glu Pro Cys Cys Asp Trp Val 
145                 150                 155                 160 


Gly Asp Ala Gly Ala Gly His Tyr Val Lys Met Val His Asn Gly Ile 
                165                 170                 175     


Glu Tyr Gly Asp Met Gln Leu Ile Cys Glu Ala Tyr Asp Leu Met Lys 
            180                 185                 190         


Arg Val Gly Gly Leu Thr Asp Lys Glu Ile Ser Asp Val Phe Gly Glu 
        195                 200                 205             


Trp Asn Glu Gly Val Leu Asp Ser Phe Leu Val Glu Ile Thr Arg Asp 
    210                 215                 220                 


Ile Leu Ala Phe Asn Asp Lys Asp Gly Thr Pro Leu Val Glu Lys Ile 
225                 230                 235                 240 


Leu Asp Thr Ala Gly Gln Lys Gly Thr Gly Lys Trp Thr Ala Ile Asn 
                245                 250                 255     


Ala Leu Asp Leu Gly Met Pro Val Thr Leu Ile Gly Glu Ala Val Phe 
            260                 265                 270         


Ala Arg Cys Leu Ser Ala Leu Lys Pro Glu Arg Glu Arg Ala Ser Glu 
        275                 280                 285             


Ile Leu Asn Gly Pro Glu Val Glu Gln Val Ser Ala Glu Gly Arg Ala 
    290                 295                 300                 


Gln Phe Ile Ala Asp Leu Met Gln Ala Leu Tyr Ala Ser Lys Ile Ile 
305                 310                 315                 320 


Ser Tyr Ala Gln Gly Phe Met Leu Ile Arg Glu Ala Ala Lys Glu Tyr 
                325                 330                 335     


Asn Trp Lys Leu Asn Phe Pro Ser Ile Ala Leu Met Trp Arg Gly Gly 
            340                 345                 350         


Cys Ile Ile Arg Ser Val Phe Leu Ala Glu Ile Thr Ala Ala Tyr Arg 
        355                 360                 365             


Glu Asn Pro Asp Leu Glu Asn Leu Leu Phe Asn Lys Phe Phe Gln Asp 
    370                 375                 380                 


Ala Ile His Lys Ala Gln Ser Gly Trp Arg Lys Thr Val Ala Leu Ala 
385                 390                 395                 400 


Val Thr Gln Gly Ile Pro Thr Pro Ala Phe Ser Thr Ala Leu Ser Phe 
                405                 410                 415     


Tyr Asp Gly Tyr Arg Ser Lys Lys Leu Pro Ala Asn Leu Leu Gln Ala 
            420                 425                 430         


Gln Arg Asp Tyr Phe Gly Ala His Thr Phe Gln Ile Leu Pro Glu Cys 
        435                 440                 445             


Ala Asp Asp Glu Lys Lys Val Gly Asp Tyr Ile His Val Asn Trp Thr 
    450                 455                 460                 


Gly Lys Gly Gly Asn Val Ser Ala Ser Thr Tyr Asp Ala 
465                 470                 475         


<210>  89
<211>  1434
<212>  DNA
<213>  Issatchenkia orientalis

<400>  89
atgggtcaaa acttgattct taatgcagca gatcatggtt ttactgttgt tgcatacaac       60

agaactgtct ctaaagttga ccatttcctt caaaatgaag caaagggtaa atccattatt      120

ggtgcacact ccattgaaga attatgtgct aagttgaaga aaccaagaag aattatgttg      180

ttagtcaagg caggtaatcc agttgatcaa ttcattgaac aattgttacc tcatttagat      240

gaaggcgata tcattattga cggtggtaac tctcacttcc ctgactccaa cagaagatac      300

gaggaattaa agaagaaggg tattctcttt gtcggttctg gtgtttctgg tggtgaagaa      360

ggtgcaagat atggtccttc tttgatgcct ggtggtgcaa aggaagcatg gcctcatatt      420

aaggacatct tccaatctat ctctgcaaag gccgatggtg agccatgttg tgattgggtt      480

ggtgatgcag gtgcaggtca ttacgttaag atggtccaca atggtatcga gtatggtgat      540

atgcagttga tctgtgaagc ttacgatttg atgaagagag ttggtggttt aactgacaag      600

gaaatatctg atgttttcgg tgaatggaac gagggtgttc tcgattcttt cttagttgaa      660

attaccagag atatcttagc tttcaacgat aaggatggta ccccattagt tgaaaagatc      720

ttagatactg ccggacagaa gggtactggt aaatggactg caataaatgc tttagacttg      780

ggtatgccag tcactttaat tggtgaagct gtttttgcga gatgtttatc cgctttgaag      840

ccagaaagag agagagcttc tgaaatctta aacggtccgg aagttgaaca agtttctgct      900

gaaggtagag cacaatttat tgcagatttg atgcaagctt tatatgcatc aaagattatt      960

tcttacgcac aaggtttcat gttaatcaga gaagcagcaa aggaatacaa ctggaaatta     1020

aacttccctt ctattgcact tatgtggaga ggtggttgta ttatcaggtc tgttttcttg     1080

gctgaaatta ctgcagctta tagggaaaac cctgacttag agaacttact attcaacaag     1140

ttcttccaag atgctattca taaggcacag tctggttgga gaaagactgt tgcattagct     1200

gttacccaag gtattccaac tccagcattc tctactgcat tgtctttcta cgatggttac     1260

agatccaaga agttaccagc taacttgttg caagcacaaa gagattactt cggtgctcac     1320

actttccaaa ttttacctga atgtgcagat gacgaaaaga aggttggtga ttacatccat     1380

gtcaactgga ctggtaaggg tggtaatgtt tctgctagta cttacgatgc ttaa           1434


<210>  90
<211>  438
<212>  PRT
<213>  Schizosaccharomyces pombe

<400>  90

Met Gly Glu Leu Lys Glu Ile Leu Lys Gln Arg Tyr His Glu Leu Leu 
1               5                   10                  15      


Asp Trp Asn Val Lys Ala Pro His Val Pro Leu Ser Gln Arg Leu Lys 
            20                  25                  30          


His Phe Thr Trp Ser Trp Phe Ala Cys Thr Met Ala Thr Gly Gly Val 
        35                  40                  45              


Gly Leu Ile Ile Gly Ser Phe Pro Phe Arg Phe Tyr Gly Leu Asn Thr 
    50                  55                  60                  


Ile Gly Lys Ile Val Tyr Ile Leu Gln Ile Phe Leu Phe Ser Leu Phe 
65                  70                  75                  80  


Gly Ser Cys Met Leu Phe Arg Phe Ile Lys Tyr Pro Ser Thr Ile Lys 
                85                  90                  95      


Asp Ser Trp Asn His His Leu Glu Lys Leu Phe Ile Ala Thr Cys Leu 
            100                 105                 110         


Leu Ser Ile Ser Thr Phe Ile Asp Met Leu Ala Ile Tyr Ala Tyr Pro 
        115                 120                 125             


Asp Thr Gly Glu Trp Met Val Trp Val Ile Arg Ile Leu Tyr Tyr Ile 
    130                 135                 140                 


Tyr Val Ala Val Ser Phe Ile Tyr Cys Val Met Ala Phe Phe Thr Ile 
145                 150                 155                 160 


Phe Asn Asn His Val Tyr Thr Ile Glu Thr Ala Ser Pro Ala Trp Ile 
                165                 170                 175     


Leu Pro Ile Phe Pro Pro Met Ile Cys Gly Val Ile Ala Gly Ala Val 
            180                 185                 190         


Asn Ser Thr Gln Pro Ala His Gln Leu Lys Asn Met Val Ile Phe Gly 
        195                 200                 205             


Ile Leu Phe Gln Gly Leu Gly Phe Trp Val Tyr Leu Leu Leu Phe Ala 
    210                 215                 220                 


Val Asn Val Leu Arg Phe Phe Thr Val Gly Leu Ala Lys Pro Gln Asp 
225                 230                 235                 240 


Arg Pro Gly Met Phe Met Phe Val Gly Pro Pro Ala Phe Ser Gly Leu 
                245                 250                 255     


Ala Leu Ile Asn Ile Ala Arg Gly Ala Met Gly Ser Arg Pro Tyr Ile 
            260                 265                 270         


Phe Val Gly Ala Asn Ser Ser Glu Tyr Leu Gly Phe Val Ser Thr Phe 
        275                 280                 285             


Met Ala Ile Phe Ile Trp Gly Leu Ala Ala Trp Cys Tyr Cys Leu Ala 
    290                 295                 300                 


Met Val Ser Phe Leu Ala Gly Phe Phe Thr Arg Ala Pro Leu Lys Phe 
305                 310                 315                 320 


Ala Cys Gly Trp Phe Ala Phe Ile Phe Pro Asn Val Gly Phe Val Asn 
                325                 330                 335     


Cys Thr Ile Glu Ile Gly Lys Met Ile Asp Ser Lys Ala Phe Gln Met 
            340                 345                 350         


Phe Gly His Ile Ile Gly Val Ile Leu Cys Ile Gln Trp Ile Leu Leu 
        355                 360                 365             


Met Tyr Leu Met Val Arg Ala Phe Leu Val Asn Asp Leu Cys Tyr Pro 
    370                 375                 380                 


Gly Lys Asp Glu Asp Ala His Pro Pro Pro Lys Pro Asn Thr Gly Val 
385                 390                 395                 400 


Leu Asn Pro Thr Phe Pro Pro Glu Lys Ala Pro Ala Ser Leu Glu Lys 
                405                 410                 415     


Val Asp Thr His Val Thr Ser Thr Gly Gly Glu Ser Asp Pro Pro Ser 
            420                 425                 430         


Ser Glu His Glu Ser Val 
        435             


<210>  91
<211>  398
<212>  PRT
<213>  Aspergillus oryzae

<400>  91

Met Phe Asn Asn Glu His His Ile Pro Pro Gly Ser Ser His Ser Asp 
1               5                   10                  15      


Ile Glu Met Leu Thr Pro Pro Lys Phe Glu Asp Glu Lys Gln Leu Gly 
            20                  25                  30          


Pro Val Gly Ile Arg Glu Arg Leu Arg His Phe Thr Trp Ala Trp Tyr 
        35                  40                  45              


Thr Leu Thr Met Ser Gly Gly Gly Leu Ala Val Leu Ile Ile Ser Gln 
    50                  55                  60                  


Pro Phe Gly Phe Arg Gly Leu Arg Glu Ile Gly Ile Ala Val Tyr Ile 
65                  70                  75                  80  


Leu Asn Leu Ile Leu Phe Ala Leu Val Cys Ser Thr Met Ala Ile Arg 
                85                  90                  95      


Phe Ile Leu His Gly Asn Leu Leu Glu Ser Leu Arg His Asp Arg Glu 
            100                 105                 110         


Gly Leu Phe Phe Pro Thr Phe Trp Leu Ser Val Ala Thr Ile Ile Cys 
        115                 120                 125             


Gly Leu Ser Arg Tyr Phe Gly Glu Glu Ser Asn Glu Ser Phe Gln Leu 
    130                 135                 140                 


Ala Leu Glu Ala Leu Phe Trp Ile Tyr Cys Val Cys Thr Leu Leu Val 
145                 150                 155                 160 


Ala Ile Ile Gln Tyr Ser Phe Val Phe Ser Ser His Lys Tyr Gly Leu 
                165                 170                 175     


Gln Thr Met Met Pro Ser Trp Ile Leu Pro Ala Phe Pro Ile Met Leu 
            180                 185                 190         


Ser Gly Thr Ile Ala Ser Val Ile Gly Glu Gln Gln Pro Ala Arg Ala 
        195                 200                 205             


Ala Leu Pro Ile Ile Gly Ala Gly Val Thr Phe Gln Gly Leu Gly Phe 
    210                 215                 220                 


Ser Ile Ser Phe Met Met Tyr Ala His Tyr Ile Gly Arg Leu Met Glu 
225                 230                 235                 240 


Ser Gly Leu Pro His Ser Asp His Arg Pro Gly Met Phe Ile Cys Val 
                245                 250                 255     


Gly Pro Pro Ala Phe Thr Ala Leu Ala Leu Val Gly Met Ser Lys Gly 
            260                 265                 270         


Leu Pro Glu Asp Phe Lys Leu Leu His Asp Ala His Ala Leu Glu Asp 
        275                 280                 285             


Gly Arg Ile Ile Glu Leu Leu Ala Ile Ser Ala Gly Val Phe Leu Trp 
    290                 295                 300                 


Ala Leu Ser Leu Trp Phe Phe Cys Ile Ala Ile Val Ala Val Ile Arg 
305                 310                 315                 320 


Ser Pro Pro Glu Ala Phe His Leu Asn Trp Trp Ala Met Val Phe Pro 
                325                 330                 335     


Asn Thr Gly Phe Thr Leu Ala Thr Ile Thr Leu Gly Lys Ala Leu Asn 
            340                 345                 350         


Ser Asn Gly Val Lys Gly Val Gly Ser Ala Met Ser Ile Cys Ile Val 
        355                 360                 365             


Cys Met Tyr Ile Phe Val Phe Val Asn Asn Val Arg Ala Val Ile Arg 
    370                 375                 380                 


Lys Asp Ile Met Tyr Pro Gly Lys Asp Glu Asp Val Ser Asp 
385                 390                 395             


<210>  92
<211>  1317
<212>  DNA
<213>  Schizosaccharomyces pombe

<400>  92
atgggtgaat tgaaagagat tttgaaacaa agatatcatg aattacttga ttggaatgtt       60

aaggcaccac atgtcccttt atcccagaga ttgaagcact ttacttggtc atggtttgct      120

tgtactatgg caaccggtgg tgttggtttg atcattggtt ccttcccatt cagattctac      180

ggtttgaaca ccattggcaa gattgtttac atcttacaaa tctttttgtt ttctcttttt      240

ggctcttgta tgttgtttcg tttcatcaag tatccatcta ccattaagga ctcttggaat      300

catcacttgg aaaagttgtt tatcgcaact tgtttgttat ctatttccac attcatcgac      360

atgttagcta tctatgctta tccagatacc ggtgaatgga tggtctgggt cattagaatc      420

ttatactaca tctatgtcgc tgtctctttc atctactgtg ttatggcctt tttcaccatt      480

ttcaacaatc atgtttacac tattgaaact gcttctccag cttggatttt gccaatcttc      540

cctccaatga tctgtggtgt cattgctggt gctgttaact ccacccaacc tgctcaccaa      600

ttgaaaaaca tggtcatttt cggtatcttg tttcaaggtt taggtttttg ggtttacctt      660

ttacttttcg ccgttaatgt tttgagattc ttcacagtcg gtttagcaaa gccacaagat      720

agaccaggta tgtttatgtt cgttggtcca ccagctttct ctggtttagc attgattaac      780

attgcaagag gtgcaatggg ctcaagacct tacattttcg ttggtgcaaa ctcttccgaa      840

tacttaggtt ttgtctcaac cttcatggcc attttcatct ggggtttagc cgcatggtgt      900

tattgcttag ctatggtttc cttccttgcc ggctttttca ctagagcacc attgaaattc      960

gcttgtggtt ggttcgcttt catctttcca aatgttggtt ttgttaactg tactatcgaa     1020

atcggcaaga tgattgattc taaggctttt caaatgtttg gtcacatcat tggtgttatc     1080

ttgtgtattc aatggatttt gttaatgtac ttaatggtta gagcattcct tgttaatgac     1140

ttgtgctatc ctggtaaaga cgaagatgca cacccaccac caaagccaaa cactggtgtc     1200

ttaaacccaa ctttcccacc agagaaggct ccagcatcat tagagaaggt tgatactcat     1260

gttacatcaa caggtggtga atccgatcct ccatcttccg aacatgaatc cgtttaa        1317


<210>  93
<211>  1205
<212>  DNA
<213>  Aspergillus oryzae

<400>  93
atgtttaaca atgagcacca tattcctcct ggttcctctc actctgatat cgaaatgtta       60

acaccaccaa agtttgagga tgaaaaacag ttaggtccag tcggtattag agaaagattg      120

agacatttca cttgggcttg gtatacctta accatgtccg gtggtggttt ggcagttttg      180

attatctctc agccattcgg ttttagaggt ttaagagaaa ttggtattgc agtttacatt      240

ttgaacttaa tcttattcgc tttggtttgt tctaccatgg ctattcgttt catcttgcac      300

ggtaaccttt tggaatccct tagacatgac agagaaggtt tgtttttccc tactttctgg      360

ttgtctgttg ctaccatcat ttgtggtttg tcaagatact ttggtgagga atccaacgaa      420

tccttccaat tggcattaga agccttgttc tggatctatt gcgtttgtac cttgttggtt      480

gcaatcattc aatactcttt tgttttctca tcccacaagt acggtttaca aacaatgatg      540

ccatcttgga ttttgccagc ctttcctatc atgttgtcag gcacaattgc atctgttatc      600

ggtgaacaac aaccagccag agctgcatta ccaatcattg gtgccggtgt caccttccaa      660

ggtttaggtt tttctatttc cttcatgatg tatgctcatt acattggcag acttatggaa      720

tccggtttac ctcactccga ccatagacca ggcatgttca tctgtgttgg cccaccagcc      780

tttactgctt tggctttagt cggtatgtcc aagggtttac cagaagattt caagctttta      840

catgacgctc atgcattaga ggatggtaga atcattgaat tgttagcaat ttcagcaggt      900

gttttccttt gggcattatc cctttggttt ttctgtattg ctattgtcgc tgtcattaga      960

tctccaccag aagctttcca cttgaactgg tgggctatgg ttttcccaaa tactggtttc     1020

accttagcta ctatcacttt gggtaaagct ttgaactcaa atggtgtcaa gggtgtcggt     1080

tctgcaatgt ccatttgtat tgtctgcatg tacatctttg ttttcgttaa caatgttaga     1140

gctgttattc gtaaggatat catgtatcca ggcaaagatg aggatgtttc tgattaacct     1200

gcagg                                                                 1205


<210>  94
<211>  1180
<212>  PRT
<213>  Issatchenkia orientalis

<400>  94

Met Ser Thr Val Glu Asp His Ser Ser Leu His Lys Leu Arg Lys Glu 
1               5                   10                  15      


Ser Glu Ile Leu Ser Asn Ala Asn Lys Ile Leu Val Ala Asn Arg Gly 
            20                  25                  30          


Glu Ile Pro Ile Arg Ile Phe Arg Ser Ala His Glu Leu Ser Met His 
        35                  40                  45              


Thr Val Ala Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu 
    50                  55                  60                  


Lys Ala Asp Glu Ala Tyr Ala Ile Gly Lys Thr Gly Gln Tyr Ser Pro 
65                  70                  75                  80  


Val Gln Ala Tyr Leu Gln Ile Asp Glu Ile Ile Lys Ile Ala Lys Glu 
                85                  90                  95      


His Asp Val Ser Met Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn 
            100                 105                 110         


Ser Glu Phe Ala Lys Lys Val Glu Glu Ser Gly Met Ile Trp Val Gly 
        115                 120                 125             


Pro Pro Ala Glu Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg 
    130                 135                 140                 


Asn Leu Ala Ile Lys Cys Asp Val Pro Val Val Pro Gly Thr Asp Gly 
145                 150                 155                 160 


Pro Ile Glu Asp Ile Glu Gln Ala Lys Gln Phe Val Glu Gln Tyr Gly 
                165                 170                 175     


Tyr Pro Val Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met 
            180                 185                 190         


Arg Val Val Arg Glu Gly Asp Asp Ile Val Asp Ala Phe Gln Arg Ala 
        195                 200                 205             


Ser Ser Glu Ala Lys Ser Ala Phe Gly Asn Gly Thr Cys Phe Ile Glu 
    210                 215                 220                 


Arg Phe Leu Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp 
225                 230                 235                 240 


Asn Tyr Gly Asn Thr Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln 
                245                 250                 255     


Arg Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Lys Thr Leu Pro 
            260                 265                 270         


Val Glu Val Arg Asn Ala Ile Leu Lys Asp Ala Val Thr Leu Ala Lys 
        275                 280                 285             


Thr Ala Asn Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Ser 
    290                 295                 300                 


Gln Asn Arg His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu 
305                 310                 315                 320 


His Thr Ile Thr Glu Glu Ile Thr Gly Val Asp Ile Val Ala Ala Gln 
                325                 330                 335     


Ile Gln Ile Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln 
            340                 345                 350         


Asn Lys Ile Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr 
        355                 360                 365             


Glu Asp Pro Ala Lys Asn Phe Ala Pro Asp Thr Gly Lys Ile Glu Val 
    370                 375                 380                 


Tyr Arg Ser Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Gly 
385                 390                 395                 400 


Phe Ala Gly Ala Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys 
                405                 410                 415     


Cys Ser Thr Ser Gly Ser Asn Tyr Glu Ile Ala Arg Arg Lys Met Ile 
            420                 425                 430         


Arg Ala Leu Val Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro 
        435                 440                 445             


Phe Leu Leu Ala Leu Leu Thr His Pro Val Phe Ile Ser Gly Asp Cys 
    450                 455                 460                 


Trp Thr Thr Phe Ile Asp Asp Thr Pro Ser Leu Phe Glu Met Val Ser 
465                 470                 475                 480 


Ser Lys Asn Arg Ala Gln Lys Leu Leu Ala Tyr Ile Gly Asp Leu Cys 
                485                 490                 495     


Val Asn Gly Ser Ser Ile Lys Gly Gln Ile Gly Phe Pro Lys Leu Asn 
            500                 505                 510         


Lys Glu Ala Glu Ile Pro Asp Leu Leu Asp Pro Asn Asp Glu Val Ile 
        515                 520                 525             


Asp Val Ser Lys Pro Ser Thr Asn Gly Leu Arg Pro Tyr Leu Leu Lys 
    530                 535                 540                 


Tyr Gly Pro Asp Ala Phe Ser Lys Lys Val Arg Glu Phe Asp Gly Cys 
545                 550                 555                 560 


Met Ile Met Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala 
                565                 570                 575     


Thr Arg Val Arg Thr Ile Asp Leu Leu Arg Ile Ala Pro Thr Thr Ser 
            580                 585                 590         


His Ala Leu Gln Asn Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr 
        595                 600                 605             


Phe Asp Val Ala Met Arg Phe Leu Tyr Glu Asp Pro Trp Glu Arg Leu 
    610                 615                 620                 


Arg Gln Leu Arg Lys Ala Val Pro Asn Ile Pro Phe Gln Met Leu Leu 
625                 630                 635                 640 


Arg Gly Ala Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile 
                645                 650                 655     


Asp His Phe Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg 
            660                 665                 670         


Val Phe Asp Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp 
        675                 680                 685             


Ala Val Lys Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Tyr Ser 
    690                 695                 700                 


Gly Asp Met Leu Ile Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu 
705                 710                 715                 720 


Glu Thr Val Gly Lys Ile Val Glu Met Gly Thr His Ile Leu Gly Ile 
                725                 730                 735     


Lys Asp Met Ala Gly Thr Leu Lys Pro Lys Ala Ala Lys Leu Leu Ile 
            740                 745                 750         


Gly Ser Ile Arg Ser Lys Tyr Pro Asp Leu Val Ile His Val His Thr 
        755                 760                 765             


His Asp Ser Ala Gly Thr Gly Ile Ser Thr Tyr Val Ala Cys Ala Leu 
    770                 775                 780                 


Ala Gly Ala Asp Ile Val Asp Cys Ala Ile Asn Ser Met Ser Gly Leu 
785                 790                 795                 800 


Thr Ser Gln Pro Ser Met Ser Ala Phe Ile Ala Ala Leu Asp Gly Asp 
                805                 810                 815     


Ile Glu Thr Gly Val Pro Glu His Phe Ala Arg Gln Leu Asp Ala Tyr 
            820                 825                 830         


Trp Ala Glu Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys 
        835                 840                 845             


Gly Pro Asp Pro Glu Val Tyr Lys His Glu Ile Pro Gly Gly Gln Leu 
    850                 855                 860                 


Thr Asn Leu Ile Phe Gln Ala Gln Gln Val Gly Leu Gly Glu Gln Trp 
865                 870                 875                 880 


Glu Glu Thr Lys Lys Lys Tyr Glu Asp Ala Asn Met Leu Leu Gly Asp 
                885                 890                 895     


Ile Val Lys Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln 
            900                 905                 910         


Phe Met Val Ser Asn Lys Leu Glu Lys Glu Asp Val Glu Lys Leu Ala 
        915                 920                 925             


Asn Glu Leu Asp Phe Pro Asp Ser Val Leu Asp Phe Phe Glu Gly Leu 
    930                 935                 940                 


Met Gly Thr Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Thr Asn Val 
945                 950                 955                 960 


Ile Ser Gly Lys Arg Arg Lys Leu Lys Gly Arg Pro Gly Leu Glu Leu 
                965                 970                 975     


Glu Pro Phe Asn Leu Glu Glu Ile Arg Glu Asn Leu Val Ser Arg Phe 
            980                 985                 990         


Gly Pro Gly Ile Thr Glu Cys Asp  Val Ala Ser Tyr Asn  Met Tyr Pro 
        995                 1000                 1005             


Lys Val  Tyr Glu Gln Tyr Arg  Lys Val Val Glu Lys  Tyr Gly Asp 
    1010                 1015                 1020             


Leu Ser  Val Leu Pro Thr Lys  Ala Phe Leu Ala Pro  Pro Thr Ile 
    1025                 1030                 1035             


Gly Glu  Glu Val His Val Glu  Ile Glu Gln Gly Lys  Thr Leu Ile 
    1040                 1045                 1050             


Ile Lys  Leu Leu Ala Ile Ser  Asp Leu Ser Lys Ser  His Gly Thr 
    1055                 1060                 1065             


Arg Glu  Val Tyr Phe Glu Leu  Asn Gly Glu Met Arg  Lys Val Thr 
    1070                 1075                 1080             


Ile Glu  Asp Lys Thr Ala Ala  Ile Glu Thr Val Thr  Arg Ala Lys 
    1085                 1090                 1095             


Ala Asp  Gly His Asn Pro Asn  Glu Val Gly Ala Pro  Met Ala Gly 
    1100                 1105                 1110             


Val Val  Val Glu Val Arg Val  Lys His Gly Thr Glu  Val Lys Lys 
    1115                 1120                 1125             


Gly Asp  Pro Leu Ala Val Leu  Ser Ala Met Lys Met  Glu Met Val 
    1130                 1135                 1140             


Ile Ser  Ala Pro Val Ser Gly  Arg Val Gly Glu Val  Phe Val Asn 
    1145                 1150                 1155             


Glu Gly  Asp Ser Val Asp Met  Gly Asp Leu Leu Val  Lys Ile Ala 
    1160                 1165                 1170             


Lys Asp  Glu Ala Pro Ala Ala  
    1175                 1180 


<210>  95
<211>  1178
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  95

Met Ser Gln Arg Lys Phe Ala Gly Leu Arg Asp Asn Phe Asn Leu Leu 
1               5                   10                  15      


Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro Ile 
            20                  25                  30          


Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Gln Thr Val Ala Ile 
        35                  40                  45              


Tyr Ser His Glu Asp Arg Leu Ser Thr His Lys Gln Lys Ala Asp Glu 
    50                  55                  60                  


Ala Tyr Val Ile Gly Glu Val Gly Gln Tyr Thr Pro Val Gly Ala Tyr 
65                  70                  75                  80  


Leu Ala Ile Asp Glu Ile Ile Ser Ile Ala Gln Lys His Gln Val Asp 
                85                  90                  95      


Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe Ala 
            100                 105                 110         


Asp Lys Val Val Lys Ala Gly Ile Thr Trp Ile Gly Pro Pro Ala Glu 
        115                 120                 125             


Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala Ala 
    130                 135                 140                 


Lys Ala Asn Val Pro Thr Val Pro Gly Thr Pro Gly Pro Ile Glu Thr 
145                 150                 155                 160 


Val Glu Glu Ala Leu Asp Phe Val Asn Glu Tyr Gly Tyr Pro Val Ile 
                165                 170                 175     


Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val Arg 
            180                 185                 190         


Glu Gly Asp Asp Val Ala Asp Ala Phe Gln Arg Ala Thr Ser Glu Ala 
        195                 200                 205             


Arg Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu Asp 
    210                 215                 220                 


Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp Asn His Gly Asn 
225                 230                 235                 240 


Val Val His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His Gln 
                245                 250                 255     


Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Arg Glu Val Arg 
            260                 265                 270         


Asp Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Cys Gly Tyr 
        275                 280                 285             


Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg His 
    290                 295                 300                 


Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile Thr 
305                 310                 315                 320 


Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile Ala 
                325                 330                 335     


Ala Gly Ala Ser Leu Pro Gln Leu Gly Leu Phe Gln Asp Lys Ile Thr 
            340                 345                 350         


Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro Ala 
        355                 360                 365             


Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Glu Val Tyr Arg Ser Ala 
    370                 375                 380                 


Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Tyr Ala Gly Thr 
385                 390                 395                 400 


Ile Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys Ser 
                405                 410                 415     


Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Ile Arg Ala Leu Ile 
            420                 425                 430         


Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu Thr 
        435                 440                 445             


Leu Leu Thr Asn Pro Val Phe Ile Glu Gly Thr Tyr Trp Thr Thr Phe 
    450                 455                 460                 


Ile Asp Asp Thr Pro Gln Leu Phe Gln Met Val Ser Ser Gln Asn Arg 
465                 470                 475                 480 


Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Val Ala Val Asn Gly Ser 
                485                 490                 495     


Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Ser Asn Pro Ser 
            500                 505                 510         


Val Pro His Leu His Asp Ala Gln Gly Asn Val Ile Asn Val Thr Lys 
        515                 520                 525             


Ser Ala Pro Pro Ser Gly Trp Arg Gln Val Leu Leu Glu Lys Gly Pro 
    530                 535                 540                 


Ala Glu Phe Ala Arg Gln Val Arg Gln Phe Asn Gly Thr Leu Leu Met 
545                 550                 555                 560 


Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val 
                565                 570                 575     


Arg Thr His Asp Leu Ala Thr Ile Ala Pro Thr Thr Ala His Ala Leu 
            580                 585                 590         


Ala Gly Arg Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val 
        595                 600                 605             


Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Lys Leu 
    610                 615                 620                 


Arg Ser Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala 
625                 630                 635                 640 


Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe 
                645                 650                 655     


Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp 
            660                 665                 670         


Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp Ala Val Lys 
        675                 680                 685             


Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Phe Ser Gly Asp Met 
    690                 695                 700                 


Leu Gln Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Glu Ile Ala 
705                 710                 715                 720 


Glu Lys Ile Val Gln Met Gly Thr His Ile Leu Gly Ile Lys Asp Met 
                725                 730                 735     


Ala Gly Thr Met Lys Pro Ala Ala Ala Lys Leu Leu Ile Gly Ser Leu 
            740                 745                 750         


Arg Ala Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser 
        755                 760                 765             


Ala Gly Thr Ala Val Ala Ser Met Thr Ala Cys Ala Leu Ala Gly Ala 
    770                 775                 780                 


Asp Val Val Asp Val Ala Ile Asn Ser Met Ser Gly Leu Thr Ser Gln 
785                 790                 795                 800 


Pro Ser Ile Asn Ala Leu Leu Ala Ser Leu Glu Gly Asn Ile Asp Thr 
                805                 810                 815     


Gly Ile Asn Val Glu His Val Arg Glu Leu Asp Ala Tyr Trp Ala Glu 
            820                 825                 830         


Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp 
        835                 840                 845             


Pro Glu Val Tyr Gln His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu 
    850                 855                 860                 


Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr 
865                 870                 875                 880 


Lys Arg Ala Tyr Arg Glu Ala Asn Tyr Leu Leu Gly Asp Ile Val Lys 
                885                 890                 895     


Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val 
            900                 905                 910         


Ser Asn Lys Leu Thr Ser Asp Asp Val Arg Arg Leu Ala Asn Ser Leu 
        915                 920                 925             


Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln 
    930                 935                 940                 


Pro Tyr Gly Gly Phe Pro Glu Pro Phe Arg Ser Asp Val Leu Arg Asn 
945                 950                 955                 960 


Lys Arg Arg Lys Leu Thr Cys Arg Pro Gly Leu Glu Leu Glu Pro Phe 
                965                 970                 975     


Asp Leu Glu Lys Ile Arg Glu Asp Leu Gln Asn Arg Phe Gly Asp Val 
            980                 985                 990         


Asp Glu Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Arg  Val Tyr Glu 
        995                 1000                 1005             


Asp Phe  Gln Lys Met Arg Glu  Thr Tyr Gly Asp Leu  Ser Val Leu 
    1010                 1015                 1020             


Pro Thr  Arg Ser Phe Leu Ser  Pro Leu Glu Thr Asp  Glu Glu Ile 
    1025                 1030                 1035             


Glu Val  Val Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Leu Gln 
    1040                 1045                 1050             


Ala Val  Gly Asp Leu Asn Lys  Lys Thr Gly Glu Arg  Glu Val Tyr 
    1055                 1060                 1065             


Phe Asp  Leu Asn Gly Glu Met  Arg Lys Ile Arg Val  Ala Asp Arg 
    1070                 1075                 1080             


Ser Gln  Lys Val Glu Thr Val  Thr Lys Ser Lys Ala  Asp Met His 
    1085                 1090                 1095             


Asp Pro  Leu His Ile Gly Ala  Pro Met Ala Gly Val  Ile Val Glu 
    1100                 1105                 1110             


Val Lys  Val His Lys Gly Ser  Leu Ile Lys Lys Gly  Gln Pro Val 
    1115                 1120                 1125             


Ala Val  Leu Ser Ala Met Lys  Met Glu Met Ile Ile  Ser Ser Pro 
    1130                 1135                 1140             


Ser Asp  Gly Gln Val Lys Glu  Val Phe Val Ser Asp  Gly Glu Asn 
    1145                 1150                 1155             


Val Asp  Ser Ser Asp Leu Leu  Val Leu Leu Glu Asp  Gln Val Pro 
    1160                 1165                 1170             


Val Glu  Thr Lys Ala 
    1175             


<210>  96
<211>  1175
<212>  PRT
<213>  Kluyvermyces marxianus

<400>  96

Met Ser Thr Gln Asn Asp Leu Ala Gly Leu Arg Asp Asn Ser Asn Leu 
1               5                   10                  15      


Leu Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro 
            20                  25                  30          


Ile Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Lys Thr Val Ala 
        35                  40                  45              


Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu Lys Ala Asp 
    50                  55                  60                  


Glu Ala Tyr Val Ile Gly Glu Pro Gly Lys Tyr Thr Pro Val Gly Ala 
65                  70                  75                  80  


Tyr Leu Ala Ile Asp Glu Ile Ile Lys Ile Ala Gln Leu His Gly Val 
                85                  90                  95      


Ser Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe 
            100                 105                 110         


Ala Lys Lys Val Ala Asp Ser Gly Ile Thr Trp Val Gly Pro Pro Ala 
        115                 120                 125             


Asp Val Ile Asp Ala Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala 
    130                 135                 140                 


Glu Arg Ala Asp Val Pro Val Val Pro Gly Thr Pro Gly Pro Ile Glu 
145                 150                 155                 160 


Thr Val Glu Glu Ala Val Glu Phe Val Glu Lys Tyr Gly Tyr Pro Val 
                165                 170                 175     


Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val 
            180                 185                 190         


Arg Glu Gly Asp Asp Ile Ala Asp Ala Phe Gln Arg Ala Lys Ser Glu 
        195                 200                 205             


Ala Val Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu 
    210                 215                 220                 


Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp His Tyr Gly 
225                 230                 235                 240 


Asn Val Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His 
                245                 250                 255     


Gln Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Glu Ser Val 
            260                 265                 270         


Arg Asn Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Ala Gly 
        275                 280                 285             


Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg 
    290                 295                 300                 


His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile 
305                 310                 315                 320 


Thr Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile 
                325                 330                 335     


Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln Asp Arg Ile 
            340                 345                 350         


Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro 
        355                 360                 365             


Ser Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Asp Val Tyr Arg Ser 
    370                 375                 380                 


Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Phe Ala Gly 
385                 390                 395                 400 


Ser Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys 
                405                 410                 415     


Ser Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Leu Arg Ala Leu 
            420                 425                 430         


Ile Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu 
        435                 440                 445             


Thr Leu Leu Thr His Pro Val Phe Lys Ser Gly Asp Tyr Trp Thr Thr 
    450                 455                 460                 


Phe Ile Asp Asp Thr Pro Gln Leu Phe Glu Met Val Ser Ser Gln Asn 
465                 470                 475                 480 


Arg Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Leu Ala Val Asn Gly 
                485                 490                 495     


Ser Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Thr His Pro 
            500                 505                 510         


Thr Ile Pro His Leu His Lys Ala Asp Gly Ser Ile Leu Asp Val Ser 
        515                 520                 525             


Ala Lys Pro Pro Ala Gly Trp Arg Asp Val Leu Leu Gln His Gly Pro 
    530                 535                 540                 


Glu Glu Phe Ala Lys Gln Val Arg Lys Phe Lys Gly Thr Leu Leu Met 
545                 550                 555                 560 


Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val 
                565                 570                 575     


Arg Thr Tyr Asp Leu Ala Ala Ile Ala Pro Thr Thr Ala His Ala Leu 
            580                 585                 590         


Ser Gly Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val 
        595                 600                 605             


Ser Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Thr Leu 
    610                 615                 620                 


Arg Lys Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala 
625                 630                 635                 640 


Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe 
                645                 650                 655     


Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp 
            660                 665                 670         


Ala Leu Asn Asp Leu Glu Gln Leu Thr Val Gly Val Asp Ala Val Lys 
        675                 680                 685             


Lys Ala Gly Gly Val Val Glu Ala Thr Ile Cys Tyr Ser Gly Asp Met 
    690                 695                 700                 


Leu Ala Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Asp Ile Val 
705                 710                 715                 720 


Glu Gln Val Val Lys Arg Gly Thr His Ile Leu Gly Ile Lys Asp Met 
                725                 730                 735     


Ala Gly Thr Leu Lys Pro Ser Ala Ala Lys Leu Leu Ile Gly Ser Ile 
            740                 745                 750         


Arg Thr Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser 
        755                 760                 765             


Ala Gly Thr Gly Val Ala Ser Met Ala Ala Cys Ala Phe Ala Gly Ala 
    770                 775                 780                 


Asp Val Val Asp Val Ala Thr Asn Ser Met Ser Gly Met Thr Ser Gln 
785                 790                 795                 800 


Pro Ser Val Asn Ala Leu Leu Ala Ala Leu Asp Gly Glu Ile Asp Cys 
                805                 810                 815     


Asn Val Asn Val Ser Tyr Ile Ser Gln Leu Asp Ala Tyr Trp Ala Glu 
            820                 825                 830         


Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp 
        835                 840                 845             


Pro Glu Val Tyr Val His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu 
    850                 855                 860                 


Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr 
865                 870                 875                 880 


Lys Arg Ala Tyr Arg Glu Ala Asn Leu Leu Leu Gly Asp Val Val Lys 
                885                 890                 895     


Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val 
            900                 905                 910         


Thr Asn Lys Leu Thr Ser Asp Asp Val Lys Arg Leu Ala Ser Ser Leu 
        915                 920                 925             


Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln 
    930                 935                 940                 


Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Ser Asp Val Leu Lys Asn 
945                 950                 955                 960 


Lys Arg Arg Lys Leu Thr Lys Arg Pro Gly Leu Glu Leu Ala Pro Phe 
                965                 970                 975     


Asp Leu Glu Gly Ile Lys Glu Asp Leu Thr Asn Arg Phe Gly Asp Ile 
            980                 985                 990         


Asp Asp Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Lys  Val Tyr Glu 
        995                 1000                 1005             


Asp Phe  Arg Lys Ile Arg Glu  Lys Tyr Gly Asp Leu  Ser Val Leu 
    1010                 1015                 1020             


Pro Thr  Lys Asn Phe Leu Ser  Pro Pro Ser Ile Gly  Glu Glu Ile 
    1025                 1030                 1035             


Val Val  Thr Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Pro Gln 
    1040                 1045                 1050             


Ala Ile  Gly Asp Leu Asn Lys  Glu Thr Gly Ile Arg  Glu Val Tyr 
    1055                 1060                 1065             


Phe Glu  Leu Asn Gly Glu Leu  Arg Lys Val Ser Val  Ala Asp Arg 
    1070                 1075                 1080             


Ser Gln  Lys Val Glu Thr Ile  Ser Lys Pro Lys Ala  Asp Ala His 
    1085                 1090                 1095             


Asp Pro  Phe Gln Val Gly Ser  Pro Met Ala Gly Val  Val Val Glu 
    1100                 1105                 1110             


Val Lys  Val His Lys Gly Ser  Leu Ile Ser Lys Gly  Gln Pro Val 
    1115                 1120                 1125             


Ala Val  Leu Ser Ala Met Lys  Met Glu Met Val Ile  Ser Ser Pro 
    1130                 1135                 1140             


Ser Asp  Gly Gln Val Lys Glu  Val Leu Val Lys Asp  Gly Glu Asn 
    1145                 1150                 1155             


Val Asp  Ala Ser Asp Leu Leu  Val Val Leu Glu Glu  Ala Pro Ala 
    1160                 1165                 1170             


Lys Glu  
    1175 


<210>  97
<211>  883
<212>  PRT
<213>  Escherichia coli

<400>  97

Met Asn Glu Gln Tyr Ser Ala Leu Arg Ser Asn Val Ser Met Leu Gly 
1               5                   10                  15      


Lys Val Leu Gly Glu Thr Ile Lys Asp Ala Leu Gly Glu His Ile Leu 
            20                  25                  30          


Glu Arg Val Glu Thr Ile Arg Lys Leu Ser Lys Ser Ser Arg Ala Gly 
        35                  40                  45              


Asn Asp Ala Asn Arg Gln Glu Leu Leu Thr Thr Leu Gln Asn Leu Ser 
    50                  55                  60                  


Asn Asp Glu Leu Leu Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn 
65                  70                  75                  80  


Leu Ala Asn Thr Ala Glu Gln Tyr His Ser Ile Ser Pro Lys Gly Glu 
                85                  90                  95      


Ala Ala Ser Asn Pro Glu Val Ile Ala Arg Thr Leu Arg Lys Leu Lys 
            100                 105                 110         


Asn Gln Pro Glu Leu Ser Glu Asp Thr Ile Lys Lys Ala Val Glu Ser 
        115                 120                 125             


Leu Ser Leu Glu Leu Val Leu Thr Ala His Pro Thr Glu Ile Thr Arg 
    130                 135                 140                 


Arg Thr Leu Ile His Lys Met Val Glu Val Asn Ala Cys Leu Lys Gln 
145                 150                 155                 160 


Leu Asp Asn Lys Asp Ile Ala Asp Tyr Glu His Asn Gln Leu Met Arg 
                165                 170                 175     


Arg Leu Arg Gln Leu Ile Ala Gln Ser Trp His Thr Asp Glu Ile Arg 
            180                 185                 190         


Lys Leu Arg Pro Ser Pro Val Asp Glu Ala Lys Trp Gly Phe Ala Val 
        195                 200                 205             


Val Glu Asn Ser Leu Trp Gln Gly Val Pro Asn Tyr Leu Arg Glu Leu 
    210                 215                 220                 


Asn Glu Gln Leu Glu Glu Asn Leu Gly Tyr Lys Leu Pro Val Glu Phe 
225                 230                 235                 240 


Val Pro Val Arg Phe Thr Ser Trp Met Gly Gly Asp Arg Asp Gly Asn 
                245                 250                 255     


Pro Asn Val Thr Ala Asp Ile Thr Arg His Val Leu Leu Leu Ser Arg 
            260                 265                 270         


Trp Lys Ala Thr Asp Leu Phe Leu Lys Asp Ile Gln Val Leu Val Ser 
        275                 280                 285             


Glu Leu Ser Met Val Glu Ala Thr Pro Glu Leu Leu Ala Leu Val Gly 
    290                 295                 300                 


Glu Glu Gly Ala Ala Glu Pro Tyr Arg Tyr Leu Met Lys Asn Leu Arg 
305                 310                 315                 320 


Ser Arg Leu Met Ala Thr Gln Ala Trp Leu Glu Ala Arg Leu Lys Gly 
                325                 330                 335     


Glu Glu Leu Pro Lys Pro Glu Gly Leu Leu Thr Gln Asn Glu Glu Leu 
            340                 345                 350         


Trp Glu Pro Leu Tyr Ala Cys Tyr Gln Ser Leu Gln Ala Cys Gly Met 
        355                 360                 365             


Gly Ile Ile Ala Asn Gly Asp Leu Leu Asp Thr Leu Arg Arg Val Lys 
    370                 375                 380                 


Cys Phe Gly Val Pro Leu Val Arg Ile Asp Ile Arg Gln Glu Ser Thr 
385                 390                 395                 400 


Arg His Thr Glu Ala Leu Gly Glu Leu Thr Arg Tyr Leu Gly Ile Gly 
                405                 410                 415     


Asp Tyr Glu Ser Trp Ser Glu Ala Asp Lys Gln Ala Phe Leu Ile Arg 
            420                 425                 430         


Glu Leu Asn Ser Lys Arg Pro Leu Leu Pro Arg Asn Trp Gln Pro Ser 
        435                 440                 445             


Ala Glu Thr Arg Glu Val Leu Asp Thr Cys Gln Val Ile Ala Glu Ala 
    450                 455                 460                 


Pro Gln Gly Ser Ile Ala Ala Tyr Val Ile Ser Met Ala Lys Thr Pro 
465                 470                 475                 480 


Ser Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Gly 
                485                 490                 495     


Phe Ala Met Pro Val Ala Pro Leu Phe Glu Thr Leu Asp Asp Leu Asn 
            500                 505                 510         


Asn Ala Asn Asp Val Met Thr Gln Leu Leu Asn Ile Asp Trp Tyr Arg 
        515                 520                 525             


Gly Leu Ile Gln Gly Lys Gln Met Val Met Ile Gly Tyr Ser Asp Ser 
    530                 535                 540                 


Ala Lys Asp Ala Gly Val Met Ala Ala Ser Trp Ala Gln Tyr Gln Ala 
545                 550                 555                 560 


Gln Asp Ala Leu Ile Lys Thr Cys Glu Lys Ala Gly Ile Glu Leu Thr 
                565                 570                 575     


Leu Phe His Gly Arg Gly Gly Ser Ile Gly Arg Gly Gly Ala Pro Ala 
            580                 585                 590         


His Ala Ala Leu Leu Ser Gln Pro Pro Gly Ser Leu Lys Gly Gly Leu 
        595                 600                 605             


Arg Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Tyr Gly Leu Pro 
    610                 615                 620                 


Glu Ile Thr Val Ser Ser Leu Ser Leu Tyr Thr Gly Ala Ile Leu Glu 
625                 630                 635                 640 


Ala Asn Leu Leu Pro Pro Pro Glu Pro Lys Glu Ser Trp Arg Arg Ile 
                645                 650                 655     


Met Asp Glu Leu Ser Val Ile Ser Cys Asp Val Tyr Arg Gly Tyr Val 
            660                 665                 670         


Arg Glu Asn Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu 
        675                 680                 685             


Gln Glu Leu Gly Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Arg 
    690                 695                 700                 


Pro Thr Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala 
705                 710                 715                 720 


Trp Thr Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Thr 
                725                 730                 735     


Ala Leu Gln Lys Val Val Glu Asp Gly Lys Gln Ser Glu Leu Glu Ala 
            740                 745                 750         


Met Cys Arg Asp Trp Pro Phe Phe Ser Thr Arg Leu Gly Met Leu Glu 
        755                 760                 765             


Met Val Phe Ala Lys Ala Asp Leu Trp Leu Ala Glu Tyr Tyr Asp Gln 
    770                 775                 780                 


Arg Leu Val Asp Lys Ala Leu Trp Pro Leu Gly Lys Glu Leu Arg Asn 
785                 790                 795                 800 


Leu Gln Glu Glu Asp Ile Lys Val Val Leu Ala Ile Ala Asn Asp Ser 
                805                 810                 815     


His Leu Met Ala Asp Leu Pro Trp Ile Ala Glu Ser Ile Gln Leu Arg 
            820                 825                 830         


Asn Ile Tyr Thr Asp Pro Leu Asn Val Leu Gln Ala Glu Leu Leu His 
        835                 840                 845             


Arg Ser Arg Gln Ala Glu Lys Glu Gly Gln Glu Pro Asp Pro Arg Val 
    850                 855                 860                 


Glu Gln Ala Leu Met Val Thr Ile Ala Gly Ile Ala Ala Gly Met Arg 
865                 870                 875                 880 


Asn Thr Gly 
            


<210>  98
<211>  342
<212>  PRT
<213>  Issatchenkia orinetalis

<400>  98

Met Ser Asn Val Lys Val Ala Leu Leu Gly Ala Ala Gly Gly Ile Gly 
1               5                   10                  15      


Gln Pro Leu Ala Leu Leu Leu Lys Leu Asn Pro Asn Ile Thr His Leu 
            20                  25                  30          


Ala Leu Tyr Asp Val Val His Val Pro Gly Val Ala Ala Asp Leu His 
        35                  40                  45              


His Ile Asp Thr Asp Val Val Ile Thr His His Leu Lys Asp Glu Asp 
    50                  55                  60                  


Gly Thr Ala Leu Ala Asn Ala Leu Lys Asp Ala Thr Phe Val Ile Val 
65                  70                  75                  80  


Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Gly Asp Leu Phe 
                85                  90                  95      


Thr Ile Asn Ala Gly Ile Cys Ala Glu Leu Ala Asn Ala Ile Ser Leu 
            100                 105                 110         


Asn Ala Pro Asn Ala Phe Thr Leu Val Ile Thr Asn Pro Val Asn Ser 
        115                 120                 125             


Thr Val Pro Ile Phe Lys Glu Ile Phe Ala Lys Asn Glu Ala Phe Asn 
    130                 135                 140                 


Pro Arg Arg Leu Phe Gly Val Thr Ala Leu Asp His Val Arg Ser Asn 
145                 150                 155                 160 


Thr Phe Leu Ser Glu Leu Ile Asp Gly Lys Asn Pro Gln His Phe Asp 
                165                 170                 175     


Val Thr Val Val Gly Gly His Ser Gly Asn Ser Ile Val Pro Leu Phe 
            180                 185                 190         


Ser Leu Val Lys Ala Ala Glu Asn Leu Asp Asp Glu Ile Ile Asp Ala 
        195                 200                 205             


Leu Ile His Arg Val Gln Tyr Gly Gly Asp Glu Val Val Glu Ala Lys 
    210                 215                 220                 


Ser Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Ala Asn Lys 
225                 230                 235                 240 


Phe Phe Asn Ile Leu Leu Asn Gly Tyr Trp Gly Leu Lys Lys Thr Met 
                245                 250                 255     


Ile Ser Ser Tyr Val Phe Leu Asp Asp Ser Ile Asn Gly Val Pro Gln 
            260                 265                 270         


Leu Lys Glu Asn Leu Ser Lys Leu Leu Lys Gly Ser Glu Val Glu Leu 
        275                 280                 285             


Pro Ser Tyr Leu Ala Val Pro Met Thr Tyr Gly Lys Glu Gly Ile Glu 
    290                 295                 300                 


Gln Val Phe Tyr Asp Trp Val Phe Glu Met Ser Pro Lys Glu Lys Glu 
305                 310                 315                 320 


Asn Phe Ile Thr Ala Ile Glu Tyr Ile Asp Gln Asn Ile Glu Lys Gly 
                325                 330                 335     


Leu Asn Phe Met Val Arg 
            340         


<210>  99
<211>  333
<212>  PRT
<213>  Issatchenkia orientalis

<400>  99

Met Val Lys Val Thr Ile Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Arg Leu Asn Pro Trp Ile Asp Glu Leu Ala Leu 
            20                  25                  30          


Phe Asp Ile Val Asn Thr Pro Gly Val Ser Cys Asp Leu Ser His Ile 
        35                  40                  45              


Pro Ala Ser Gln Val Val Asn Gly Tyr Ala Pro Lys Ser Lys Ser Asp 
    50                  55                  60                  


Thr Glu Thr Ile Lys Thr Ala Leu Lys Gly Ala Asp Ile Val Val Ile 
65                  70                  75                  80  


Pro Ala Gly Ile Pro Arg Lys Pro Gly Met Thr Arg Asn Asp Leu Phe 
                85                  90                  95      


Lys Ile Asn Ala Gly Ile Val Lys Ser Leu Ile His Ser Ala Gly Thr 
            100                 105                 110         


Thr Cys Pro Asp Ala Phe Ile Cys Val Ile Ser Asn Pro Val Asn Ser 
        115                 120                 125             


Thr Val Pro Ile Ala Val Glu Glu Leu Lys Arg Leu Asn Val Phe Asn 
    130                 135                 140                 


Pro His Lys Val Phe Gly Ile Thr Thr Leu Asp Asn Phe Arg Leu Glu 
145                 150                 155                 160 


Glu Phe Leu Ser Gly Glu Leu Gly Gly Ile Val Lys Pro Asn Asp Leu 
                165                 170                 175     


Tyr Gly Asp Val Val Ala Ile Gly Gly His Ser Gly Asp Ser Ile Val 
            180                 185                 190         


Pro Ile Leu Asn Ser Trp Asn Leu Asn Phe Ile Asn Asp Gly Asp Ser 
        195                 200                 205             


Tyr Asn Asn Leu Val Lys Arg Val Gln Phe Gly Gly Asp Glu Val Val 
    210                 215                 220                 


Lys Ala Lys Asp Gly Lys Gly Ser Ala Thr Leu Ser Met Ala Thr Ala 
225                 230                 235                 240 


Ala Tyr Arg Phe Val Asn Asn Leu Leu Asp Ala Ile Val Asn Asn Lys 
                245                 250                 255     


Lys Val Lys Glu Val Ala Phe Val Lys Ile Asp Gln Leu Pro Thr Thr 
            260                 265                 270         


Arg Val Pro Tyr Phe Val Val Asp Glu Thr Gln Tyr Phe Ser Leu Pro 
        275                 280                 285             


Ile Ile Leu Gly Arg Gln Gly Ile Glu Arg Val Thr Phe Pro Glu Ser 
    290                 295                 300                 


Leu Thr Glu Gln Glu Val Arg Met Thr Lys His Ala Val Ala Lys Val 
305                 310                 315                 320 


Lys Val Asp Val Asn Lys Gly Phe Asn Phe Val His Gly 
                325                 330             


<210>  100
<211>  334
<212>  PRT
<213>  Issatchenkia orientalis

<400>  100

Met Phe Ser Arg Ile Ser Ala Arg Gln Phe Ser Ser Ser Ala Ala Ser 
1               5                   10                  15      


Ala Tyr Lys Val Thr Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro 
            20                  25                  30          


Leu Ser Leu Leu Met Lys Leu Asn His Lys Val Thr Asn Leu Ser Leu 
        35                  40                  45              


Tyr Asp Leu Arg Leu Gly Ala Gly Val Ala Thr Asp Leu Ser His Ile 
    50                  55                  60                  


Pro Thr Asn Ser Val Val Lys Gly Tyr Gly Pro Glu Asn Asn Gly Leu 
65                  70                  75                  80  


Lys Asp Ala Leu Thr Gly Ser Asp Val Val Leu Ile Pro Ala Gly Val 
                85                  90                  95      


Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Asn Thr Asn Ala 
            100                 105                 110         


Ser Ile Val Arg Asp Leu Ala Lys Ala Ala Ala Asp His Cys Pro Asn 
        115                 120                 125             


Ala Val Leu Leu Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile 
    130                 135                 140                 


Val Ala Glu Val Leu Lys Ser Lys Gly Val Tyr Asn Pro Lys Lys Leu 
145                 150                 155                 160 


Phe Gly Val Thr Thr Leu Asp Val Leu Arg Ser Ser Arg Phe Leu Ser 
                165                 170                 175     


Glu Val Val Asn Thr Asp Pro Thr Thr Glu Thr Val Thr Val Val Gly 
            180                 185                 190         


Gly His Ser Gly Val Thr Ile Val Pro Leu Ile Ser Gln Thr Lys His 
        195                 200                 205             


Lys Asp Leu Pro Lys Glu Thr Tyr Glu Ala Leu Val His Arg Ile Gln 
    210                 215                 220                 


Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala 
225                 230                 235                 240 


Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Met Ala Ser Ser Val Leu 
                245                 250                 255     


Lys Gly Leu Ala Gly Glu Val Asp Ile Val Glu Pro Thr Phe Ile Asp 
            260                 265                 270         


Ser Pro Leu Phe Lys Ser Glu Gly Val Glu Phe Phe Ser Ser Arg Val 
        275                 280                 285             


Thr Leu Gly Pro Glu Gly Val Gln Glu Val His Pro Leu Gly Val Leu 
    290                 295                 300                 


Ser Thr Ala Glu Glu Glu Met Val Ala Thr Ala Lys Glu Thr Leu Lys 
305                 310                 315                 320 


Lys Asn Ile Gln Lys Gly Val Asp Phe Val Lys Ala Asn Pro 
                325                 330                 


<210>  101
<211>  356
<212>  PRT
<213>  Zygosaccharomyces rouxii

<400>  101

Met Pro His Ser Ile Asn Gly Asp Val Lys Ile Ala Val Leu Gly Ala 
1               5                   10                  15      


Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu Leu Lys Thr Gln Leu 
            20                  25                  30          


Thr Arg Glu Leu Pro Asn His Arg His Ala Gln Leu Ala Leu Tyr Asp 
        35                  40                  45              


Val Asn Ala Asp Ala Val Arg Gly Val Ala Ala Asp Leu Ser His Ile 
    50                  55                  60                  


Asp Thr Gly Val Thr Val Thr Gly Tyr Glu Gly Asp Arg Ile Gly Glu 
65                  70                  75                  80  


Ala Leu Glu Gly Thr Asp Ile Val Leu Ile Pro Ala Gly Val Pro Arg 
                85                  90                  95      


Lys Pro Gly Met Thr Arg Glu Asp Leu Leu Val Val Asn Ala Lys Ile 
            100                 105                 110         


Val Lys Ser Ile Gly Ser Ser Ile Ala Gln His Cys Asp Leu Asn Lys 
        115                 120                 125             


Val Phe Ile Leu Leu Ile Ser Asn Pro Ile Asn Ser Leu Val Pro Val 
    130                 135                 140                 


Leu Val Lys Glu Leu Glu Ser Lys Ser Gln Gly Thr Gln Val Glu Arg 
145                 150                 155                 160 


Arg Val Leu Gly Leu Thr Lys Leu Asp Ser Val Arg Ala Ser Ala Phe 
                165                 170                 175     


Leu His Glu Val Thr Ile Lys His Gly Leu Lys Pro Lys Ser Asn Thr 
            180                 185                 190         


Leu Asp Asp Val Pro Val Val Gly Gly His Ser Gly Glu Thr Ile Val 
        195                 200                 205             


Pro Leu Phe Ser Gln Ala Pro Asn Gly Asn Arg Leu Ser Gln Asp Ala 
    210                 215                 220                 


Leu Glu Ala Leu Val Gln Arg Val Gln Phe Gly Gly Asp Glu Val Val 
225                 230                 235                 240 


Arg Ala Lys Asn Gly Ala Gly Ser Ala Thr Leu Cys Met Ala His Ala 
                245                 250                 255     


Ala Tyr Thr Val Ala Ala Ser Phe Ile Pro Leu Ile Thr Gly Gln Lys 
            260                 265                 270         


Arg Ser Ile Ser Gly Thr Phe Tyr Val Ala Leu Lys Asp Ala Gln Gly 
        275                 280                 285             


Gln Pro Ile Asn Ser Ser Ala Lys Arg Leu Leu Gly Ser Ile Asn Asp 
    290                 295                 300                 


Leu Pro Tyr Phe Ala Val Pro Leu Glu Ile Thr Ser Gln Gly Val Asp 
305                 310                 315                 320 


Glu Leu Asp Thr Ser Val Leu Glu Arg Met Thr Lys Tyr Glu Arg Glu 
                325                 330                 335     


Arg Leu Leu Ala Pro Cys Leu Gly Lys Leu Glu Gly Gly Ile Arg Asn 
            340                 345                 350         


Gly Leu Ser Leu 
        355     


<210>  102
<211>  338
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  102

Met Leu Arg Ala Leu Thr Arg Arg Gln Phe Ser Ser Thr Ala Phe Asn 
1               5                   10                  15      


Pro Tyr Lys Val Thr Val Leu Gly Ala Gly Gly Gly Ile Gly Gln Pro 
            20                  25                  30          


Leu Ser Leu Leu Leu Lys Leu Asn His Lys Val Thr Asp Leu Arg Leu 
        35                  40                  45              


Tyr Asp Leu Lys Gly Ala Lys Gly Val Ala Ala Asp Leu Ser His Ile 
    50                  55                  60                  


Pro Thr Asn Ser Thr Val Thr Gly Tyr Thr Pro Glu Ser Lys Asp Ser 
65                  70                  75                  80  


Gln Glu Glu Leu Ala Ala Ala Leu Lys Asp Thr Glu Val Val Leu Ile 
                85                  90                  95      


Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe 
            100                 105                 110         


Ala Ile Asn Ala Gly Ile Val Arg Asp Leu Ala Thr Ser Ile Ala Lys 
        115                 120                 125             


Asn Ala Pro Asn Ala Ala Ile Leu Val Ile Ser Asn Pro Val Asn Ser 
    130                 135                 140                 


Thr Val Pro Ile Val Ala Glu Val Leu Lys Gln Asn Gly Val Tyr Asn 
145                 150                 155                 160 


Pro Lys Lys Leu Phe Gly Val Thr Thr Leu Asp Val Ile Arg Ala Ser 
                165                 170                 175     


Arg Phe Ile Ser Glu Val Arg Gly Thr Asp Pro Thr Thr Glu His Val 
            180                 185                 190         


Thr Val Val Gly Gly His Ser Gly Ile Thr Ile Leu Pro Leu Val Ser 
        195                 200                 205             


Gln Thr Lys His Lys Ser Val Ile Lys Gly Glu Glu Leu Asp Asn Leu 
    210                 215                 220                 


Ile His Arg Ile Gln Phe Gly Gly Asp Glu Val Val Gln Ala Lys Asn 
225                 230                 235                 240 


Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Phe 
                245                 250                 255     


Ala Asn Ser Val Leu Ser Gly Phe Glu Gly Glu Arg Asp Val Ile Glu 
            260                 265                 270         


Pro Thr Phe Val Asp Ser Pro Leu Phe Lys Asp Glu Gly Ile Glu Phe 
        275                 280                 285             


Phe Ala Ser Pro Val Thr Leu Gly Pro Glu Gly Val Glu Lys Ile His 
    290                 295                 300                 


Gly Leu Gly Val Leu Ser Asp Lys Glu Glu Gln Met Leu Ala Thr Cys 
305                 310                 315                 320 


Lys Glu Thr Leu Lys Lys Asn Ile Glu Lys Gly Gln Asn Phe Val Lys 
                325                 330                 335     


Gln Asn 
        


<210>  103
<211>  335
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  103

Met Val Ser Val Ala Val Leu Gly Ser Ser Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Lys Leu Asp Pro Arg Val Ser Ser Leu Arg Leu 
            20                  25                  30          


Tyr Asp Leu Lys Met Ser His Gly Ile Ala Thr Asp Leu Ser His Met 
        35                  40                  45              


Asp Ser Asn Ser Ile Cys Glu Gly Phe Asn Thr Asp Glu Ile Ala Leu 
    50                  55                  60                  


Ala Leu Lys Gly Ala Gln Ile Val Val Ile Pro Ala Gly Val Pro Arg 
65                  70                  75                  80  


Lys Pro Gly Met Ser Arg Asp Asp Leu Phe Lys Ile Asn Ala Lys Ile 
                85                  90                  95      


Ile Lys Ser Leu Ala Leu Gln Ile Ala Glu His Ala Pro Glu Ala Arg 
            100                 105                 110         


Val Leu Val Ile Ser Asn Pro Val Asn Ser Leu Val Pro Ile Val Tyr 
        115                 120                 125             


Glu Thr Leu Lys Ser Val Gly Lys Phe Glu Pro Gly Lys Val Met Gly 
    130                 135                 140                 


Ile Thr Thr Leu Asp Ile Ile Arg Ser His Thr Phe Leu Val Asp Val 
145                 150                 155                 160 


Leu Gly Arg Lys Ala Tyr Ser Val Glu Lys Leu Arg Ser Ala Val Thr 
                165                 170                 175     


Val Val Gly Gly His Ser Gly Glu Thr Ile Val Pro Ile Phe Thr Asp 
            180                 185                 190         


Gln Lys Phe Tyr Arg Arg Leu Arg Asp Arg Glu Leu Tyr Asp Ala Tyr 
        195                 200                 205             


Val His Arg Val Gln Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp 
    210                 215                 220                 


Gly Ser Gly Ser Ala Thr Leu Ser Met Ala Trp Ala Gly Tyr Ser Phe 
225                 230                 235                 240 


Val Lys Gln Leu Leu Asn Ser Leu His Leu Glu Thr Gly Glu Asp Val 
                245                 250                 255     


His Pro Ile Pro Thr Phe Val Tyr Leu Pro Gly Leu Pro Gly Gly Lys 
            260                 265                 270         


Glu Leu Gln Gln Lys Leu Gly Thr Ser Val Glu Phe Phe Ala Ala Pro 
        275                 280                 285             


Val Lys Leu Ser Lys Gly Ile Val Val Glu Val Glu His Asp Trp Val 
    290                 295                 300                 


Asp Lys Leu Asn Asp Ala Glu Lys Lys Leu Ile Ala Lys Cys Leu Pro 
305                 310                 315                 320 


Ile Leu Asp Lys Asn Ile Lys Lys Gly Leu Ala Phe Ser Gln Gln 
                325                 330                 335 


<210>  104
<211>  374
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  104

Met Pro Ala Val Ser Tyr Asp Val Gln Gln Arg Asp Ile Leu Lys Ile 
1               5                   10                  15      


Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu 
            20                  25                  30          


Leu Lys Ser Asn Ala Ser Phe Leu Leu Pro Arg Asp Ser Ser Arg His 
        35                  40                  45              


Ile Ser Leu Ala Leu Tyr Asp Val Asn Lys Asp Ala Ile Val Gly Thr 
    50                  55                  60                  


Ala Ala Asp Leu Ser His Ile Asp Thr Pro Ile Thr Thr Thr Pro His 
65                  70                  75                  80  


Tyr Pro Asn Asp Gly Asn Gly Gly Ile Ala Arg Cys Leu Gln Asp Ala 
                85                  90                  95      


Asp Met Val Ile Ile Pro Ala Gly Val Pro Arg Lys Pro Gly Met Ser 
            100                 105                 110         


Arg Asp Asp Leu Ile Gly Val Asn Ala Lys Ile Ile Lys Ser Leu Gly 
        115                 120                 125             


Asn Asp Ile Ala Glu Tyr Cys Asp Leu Ser Lys Val His Val Leu Val 
    130                 135                 140                 


Ile Ser Asn Pro Val Asn Ser Leu Val Pro Leu Met Val Ser Thr Leu 
145                 150                 155                 160 


Ala Asn Ser Pro His Ser Ala Asn Thr Asn Ile Glu Ala Arg Val Tyr 
                165                 170                 175     


Gly Ile Thr His Leu Asp Leu Val Arg Ala Ser Thr Phe Val Gln Gln 
            180                 185                 190         


Leu Asn Ser Phe Lys Ser Asn Asn Ala Pro Asp Ile Pro Val Ile Gly 
        195                 200                 205             


Gly His Ser Gly Asp Thr Ile Ile Pro Val Phe Ser Val Leu Asn His 
    210                 215                 220                 


Arg Ala Ser Asn Ser Gly Tyr Ala Asn Leu Leu Asp Asn Gly Val Arg 
225                 230                 235                 240 


Gln Lys Leu Val His Arg Val Gln Tyr Gly Gly Asp Glu Ile Val Gln 
                245                 250                 255     


Ala Lys Asn Gly Asn Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Gly 
            260                 265                 270         


Phe Lys Ile Ala Ala Gln Phe Ile Asp Leu Leu Val Gly Asn Ile Arg 
        275                 280                 285             


Thr Ile Glu Asn Ile Cys Met Tyr Val Pro Leu Thr Asn Arg Tyr Asn 
    290                 295                 300                 


Thr Glu Ile Ala Pro Gly Ser Asp Glu Leu Arg Ser Lys Tyr Ile Asn 
305                 310                 315                 320 


Gly Thr Leu Tyr Phe Ser Ile Pro Leu Ser Ile Gly Ile Asn Gly Ile 
                325                 330                 335     


Glu Arg Val His Tyr Glu Ile Met Glu His Leu Asp Ser Tyr Glu Arg 
            340                 345                 350         


Glu Thr Leu Leu Pro Ile Cys Leu Glu Thr Leu Lys Gly Asn Ile Asp 
        355                 360                 365             


Lys Gly Leu Ser Leu Val 
    370                 


<210>  105
<211>  312
<212>  PRT
<213>  Escherichia coli

<400>  105

Met Lys Val Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ala Leu 
1               5                   10                  15      


Ala Leu Leu Leu Lys Thr Gln Leu Pro Ser Gly Ser Glu Leu Ser Leu 
            20                  25                  30          


Tyr Asp Ile Ala Pro Val Thr Pro Gly Val Ala Val Asp Leu Ser His 
        35                  40                  45              


Ile Pro Thr Ala Val Lys Ile Lys Gly Phe Ser Gly Glu Asp Ala Thr 
    50                  55                  60                  


Pro Ala Leu Glu Gly Ala Asp Val Val Leu Ile Ser Ala Gly Val Ala 
65                  70                  75                  80  


Arg Lys Pro Gly Met Asp Arg Ser Asp Leu Phe Asn Val Asn Ala Gly 
                85                  90                  95      


Ile Val Lys Asn Leu Val Gln Gln Val Ala Lys Thr Cys Pro Lys Ala 
            100                 105                 110         


Cys Ile Gly Ile Ile Thr Asn Pro Val Asn Thr Thr Val Ala Ile Ala 
        115                 120                 125             


Ala Glu Val Leu Lys Lys Ala Gly Val Tyr Asp Lys Asn Lys Leu Phe 
    130                 135                 140                 


Gly Val Thr Thr Leu Asp Ile Ile Arg Ser Asn Thr Phe Val Ala Glu 
145                 150                 155                 160 


Leu Lys Gly Lys Gln Pro Gly Glu Val Glu Val Pro Val Ile Gly Gly 
                165                 170                 175     


His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Val 
            180                 185                 190         


Ser Phe Thr Glu Gln Glu Val Ala Asp Leu Thr Lys Arg Ile Gln Asn 
        195                 200                 205             


Ala Gly Thr Glu Val Val Glu Ala Lys Ala Gly Gly Gly Ser Ala Thr 
    210                 215                 220                 


Leu Ser Met Gly Gln Ala Ala Ala Arg Phe Gly Leu Ser Leu Val Arg 
225                 230                 235                 240 


Ala Leu Gln Gly Glu Gln Gly Val Val Glu Cys Ala Tyr Val Glu Gly 
                245                 250                 255     


Asp Gly Gln Tyr Ala Arg Phe Phe Ser Gln Pro Leu Leu Leu Gly Lys 
            260                 265                 270         


Asn Gly Val Glu Glu Arg Lys Ser Ile Gly Thr Leu Ser Ala Phe Glu 
        275                 280                 285             


Gln Asn Ala Leu Glu Gly Met Leu Asp Thr Leu Lys Lys Asp Ile Ala 
    290                 295                 300                 


Leu Gly Glu Glu Phe Val Asn Lys 
305                 310         


<210>  106
<211>  337
<212>  PRT
<213>  Rhizopus oryzae

<400>  106

Met Phe Ala Ala Ser Arg Val Phe Ser Ile Ala Ala Lys Arg Ser Phe 
1               5                   10                  15      


Ser Thr Ser Ala Ala Asn Leu Ser Lys Val Ala Val Leu Gly Ala Ala 
            20                  25                  30          


Gly Gly Ile Gly Gln Pro Leu Ser Leu Leu Leu Lys Glu Asn Pro His 
        35                  40                  45              


Val Thr His Leu Ser Leu Tyr Asp Ile Val Asn Thr Pro Gly Val Ala 
    50                  55                  60                  


Ala Asp Leu Ser His Ile Asn Thr Asn Ser Lys Val Thr Gly His Thr 
65                  70                  75                  80  


Pro Glu Asn Asp Gly Leu Lys Thr Ala Leu Glu Gly Ala His Val Val 
                85                  90                  95      


Val Ile Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Asp Asp 
            100                 105                 110         


Leu Phe Asn Thr Asn Ala Ser Ile Val Arg Asp Leu Ala Glu Ala Ala 
        115                 120                 125             


Ala Lys His Cys Pro Asp Ala His Phe Leu Ile Ile Ser Asn Pro Val 
    130                 135                 140                 


Asn Ser Thr Val Pro Ile Phe Ala Glu Thr Leu Lys Lys Ala Gly Val 
145                 150                 155                 160 


Phe Asn Pro Lys Arg Leu Tyr Gly Val Thr Thr Leu Asp Val Val Arg 
                165                 170                 175     


Ala Ser Arg Phe Val Ala Glu Val Lys Asn Leu Asp Pro Asn Asp Val 
            180                 185                 190         


Lys Val Thr Val Val Gly Gly His Ser Gly Val Thr Ile Val Pro Leu 
        195                 200                 205             


Leu Ser Gln Thr Gly Leu Glu Phe Ser Lys Glu Glu Leu Asp Ala Leu 
    210                 215                 220                 


Thr His Arg Ile Gln Phe Gly Gly Asp Glu Val Val Gln Ala Lys Asn 
225                 230                 235                 240 


Gly Thr Gly Ser Val Thr Leu Ser Met Ala Phe Ala Gly Ala Arg Phe 
                245                 250                 255     


Ala Asn Ser Val Leu Glu Ala Thr Val Gly Gly Lys Lys Gly Val Val 
            260                 265                 270         


Glu Pro Ser Phe Val Lys Ser Asp Val Phe Ala Lys Asp Gly Val Glu 
        275                 280                 285             


Tyr Phe Ser Thr Asn Ile Glu Leu Gly Pro Glu Gly Val Glu Lys Ile 
    290                 295                 300                 


Asn Glu Leu Gly Gln Ile Ser Asp Tyr Glu Lys Glu Leu Ile Ala Lys 
305                 310                 315                 320 


Ala Val Pro Glu Leu Lys Lys Asn Ile Ala Lys Gly Asn Ser Phe Val 
                325                 330                 335     


Gln 
    


<210>  107
<211>  485
<212>  PRT
<213>  Issatchenkia orientalis

<400>  107

Met Leu Ala Ala Arg Ser Leu Lys Ala Arg Met Ser Thr Arg Ala Phe 
1               5                   10                  15      


Ser Thr Thr Ser Ile Ala Lys Arg Ile Glu Lys Asp Ala Phe Gly Asp 
            20                  25                  30          


Ile Glu Val Pro Asn Glu Lys Tyr Trp Gly Ala Gln Thr Gln Arg Ser 
        35                  40                  45              


Leu Gln Asn Phe Lys Ile Gly Gly Lys Arg Glu Val Met Pro Glu Pro 
    50                  55                  60                  


Ile Ile Lys Ser Phe Gly Ile Leu Lys Lys Ala Thr Ala Lys Ile Asn 
65                  70                  75                  80  


Ala Glu Ser Gly Ala Leu Asp Pro Lys Leu Ser Glu Ala Ile Gln Gln 
                85                  90                  95      


Ala Ala Thr Glu Val Tyr Glu Gly Lys Leu Met Asp His Phe Pro Leu 
            100                 105                 110         


Val Val Phe Gln Thr Gly Ser Gly Thr Gln Ser Asn Met Asn Ala Asn 
        115                 120                 125             


Glu Val Ile Ser Asn Arg Ala Ile Glu Ile Leu Gly Gly Glu Leu Gly 
    130                 135                 140                 


Ser Lys Thr Pro Val His Pro Asn Asp His Val Asn Met Ser Gln Ser 
145                 150                 155                 160 


Ser Asn Asp Thr Phe Pro Thr Val Met His Ile Ala Ala Val Thr Glu 
                165                 170                 175     


Val Ser Ser His Leu Leu Pro Glu Leu Thr Ala Leu Arg Asp Ala Leu 
            180                 185                 190         


Gln Lys Lys Ser Asp Glu Phe Lys Asn Ile Ile Lys Ile Gly Arg Thr 
        195                 200                 205             


His Leu Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu Phe Ser Gly 
    210                 215                 220                 


Tyr Val Gln Gln Cys Thr Asn Gly Ile Lys Arg Ile Glu Ile Ala Leu 
225                 230                 235                 240 


Glu His Leu Arg Tyr Leu Ala Gln Gly Gly Thr Ala Val Gly Thr Gly 
                245                 250                 255     


Leu Asn Thr Lys Lys Gly Phe Ala Glu Lys Val Ala Asn Glu Val Thr 
            260                 265                 270         


Lys Leu Thr Gly Leu Gln Phe Tyr Thr Ala Pro Asn Lys Phe Glu Ala 
        275                 280                 285             


Leu Ala Ala His Asp Ala Val Val Glu Met Ser Gly Ala Leu Asn Thr 
    290                 295                 300                 


Val Ala Val Ser Leu Phe Lys Ile Ala Gln Asp Ile Arg Tyr Leu Gly 
305                 310                 315                 320 


Ser Gly Pro Arg Cys Gly Tyr Gly Glu Leu Ala Leu Pro Glu Asn Glu 
                325                 330                 335     


Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr Gln Asn Glu 
            340                 345                 350         


Ala Leu Thr Met Leu Cys Thr Gln Val Phe Gly Asn His Ser Cys Ile 
        355                 360                 365             


Thr Phe Ala Gly Ala Ser Gly Gln Phe Glu Leu Asn Val Phe Lys Pro 
    370                 375                 380                 


Val Met Ile Ser Asn Leu Leu Ser Ser Ile Arg Leu Leu Gly Asp Gly 
385                 390                 395                 400 


Cys Asn Ser Phe Arg Ile His Cys Val Glu Gly Ile Ile Ala Asn Thr 
                405                 410                 415     


Asp Lys Ile Asp Lys Leu Leu His Glu Ser Leu Met Leu Val Thr Ala 
            420                 425                 430         


Leu Asn Pro His Ile Gly Tyr Asp Lys Ala Ser Lys Ile Ala Lys Asn 
        435                 440                 445             


Ala His Lys Lys Gly Leu Thr Leu Lys Gln Ser Ala Leu Glu Leu Gly 
    450                 455                 460                 


Tyr Leu Thr Glu Glu Gln Phe Asn Glu Trp Val Arg Pro Glu Asn Met 
465                 470                 475                 480 


Ile Gly Pro Lys Asp 
                485 


<210>  108
<211>  470
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  108

Met Ser Leu Ser Pro Val Val Val Ile Gly Thr Gly Leu Ala Gly Leu 
1               5                   10                  15      


Ala Ala Ala Asn Glu Leu Val Asn Lys Tyr Asn Ile Pro Val Thr Ile 
            20                  25                  30          


Leu Glu Lys Ala Ser Ser Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser 
        35                  40                  45              


Gly Ile Asn Gly Ala Cys Thr Glu Thr Gln Arg His Phe His Ile Glu 
    50                  55                  60                  


Asp Ser Pro Arg Leu Phe Glu Asp Asp Thr Ile Lys Ser Ala Lys Gly 
65                  70                  75                  80  


Lys Gly Val Gln Glu Leu Met Ala Lys Leu Ala Asn Asp Ser Pro Leu 
                85                  90                  95      


Ala Ile Glu Trp Leu Lys Asn Glu Phe Asp Leu Lys Leu Asp Leu Leu 
            100                 105                 110         


Ala Gln Leu Gly Gly His Ser Val Ala Arg Thr His Arg Ser Ser Gly 
        115                 120                 125             


Lys Leu Pro Pro Gly Phe Glu Ile Val Ser Ala Leu Ser Asn Asn Leu 
    130                 135                 140                 


Lys Lys Leu Ala Glu Thr Lys Pro Glu Leu Val Lys Ile Asn Leu Asp 
145                 150                 155                 160 


Ser Lys Val Val Asp Ile His Glu Lys Asp Gly Ser Ile Ser Ala Val 
                165                 170                 175     


Val Tyr Glu Asp Lys Asn Gly Glu Lys His Met Val Ser Ala Asn Asp 
            180                 185                 190         


Val Val Phe Cys Ser Gly Gly Phe Gly Phe Ser Lys Glu Met Leu Lys 
        195                 200                 205             


Glu Tyr Ala Pro Glu Leu Val Asn Leu Pro Thr Thr Asn Gly Gln Gln 
    210                 215                 220                 


Thr Thr Gly Asp Gly Gln Arg Leu Leu Gln Lys Leu Gly Ala Asp Leu 
225                 230                 235                 240 


Ile Asp Met Asp Gln Ile Gln Val His Pro Thr Gly Phe Ile Asp Pro 
                245                 250                 255     


Asn Asp Arg Ser Ser Ser Trp Lys Phe Leu Ala Ala Glu Ser Leu Arg 
            260                 265                 270         


Gly Leu Gly Gly Ile Leu Leu Asn Pro Ile Thr Gly Arg Arg Phe Val 
        275                 280                 285             


Asn Glu Leu Thr Thr Arg Asp Val Val Thr Ala Ala Ile Gln Lys Val 
    290                 295                 300                 


Cys Pro Gln Glu Asp Asn Arg Ala Leu Leu Val Met Gly Glu Lys Met 
305                 310                 315                 320 


Tyr Thr Asp Leu Lys Asn Asn Leu Asp Phe Tyr Met Phe Lys Lys Leu 
                325                 330                 335     


Val Gln Lys Leu Thr Leu Ser Gln Val Val Ser Glu Tyr Asn Leu Pro 
            340                 345                 350         


Ile Thr Val Ala Gln Leu Cys Glu Glu Leu Gln Thr Tyr Ser Ser Phe 
        355                 360                 365             


Thr Thr Lys Ala Asp Pro Leu Gly Arg Thr Val Ile Leu Asn Glu Phe 
    370                 375                 380                 


Gly Ser Asp Val Thr Pro Glu Thr Val Val Phe Ile Gly Glu Val Thr 
385                 390                 395                 400 


Pro Val Val His Phe Thr Met Gly Gly Ala Arg Ile Asn Val Lys Ala 
                405                 410                 415     


Gln Val Ile Gly Lys Asn Asp Glu Arg Leu Leu Lys Gly Leu Tyr Ala 
            420                 425                 430         


Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu Gly Gly 
        435                 440                 445             


Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Thr Ala Ala Glu Ser 
    450                 455                 460                 


Ile Ala Asn Asp Arg Lys 
465                 470 


<210>  109
<211>  470
<212>  PRT
<213>  Saccharomyces mitakae

<400>  109

Met Ser Ser Ser Pro Val Val Val Ile Gly Thr Gly Leu Ala Gly Leu 
1               5                   10                  15      


Ala Thr Ala Asn Glu Leu Val Asn Lys Tyr Asn Ile Pro Val Thr Ile 
            20                  25                  30          


Leu Glu Lys Ala Ser Ser Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser 
        35                  40                  45              


Gly Ile Asn Gly Ala Cys Thr Glu Thr Gln Arg His Phe His Ile Glu 
    50                  55                  60                  


Asp Thr Pro Arg Leu Phe Glu Asp Asp Thr Val Gln Ser Ala Lys Gly 
65                  70                  75                  80  


Lys Gly Val Gln Glu Leu Met Gly Lys Leu Ala Asn Asp Ser Pro Leu 
                85                  90                  95      


Ala Ile Glu Trp Leu Lys Thr Glu Phe Asp Leu Lys Leu Asp Leu Leu 
            100                 105                 110         


Ala Gln Leu Gly Gly His Ser Val Ala Arg Thr His Arg Ser Ser Gly 
        115                 120                 125             


Lys Leu Pro Pro Gly Phe Glu Ile Val Ser Ala Leu Ser Asn Asn Leu 
    130                 135                 140                 


Lys Lys Leu Ala Glu Thr Lys Pro Glu Leu Val Lys Ile Asn Leu Asp 
145                 150                 155                 160 


Ser Lys Val Val Asp Ile His Lys Lys Asp Gly Ser Ile Ser Ala Ile 
                165                 170                 175     


Val Tyr Asp Asp Lys Asn Gly Glu Arg His Thr Leu Ser Thr Ser Asn 
            180                 185                 190         


Val Val Phe Cys Ser Gly Gly Phe Gly Phe Ser Lys Glu Met Leu Asn 
        195                 200                 205             


Glu Tyr Ala Pro Gln Leu Val Asn Leu Pro Thr Thr Asn Gly Gln Gln 
    210                 215                 220                 


Thr Thr Gly Asp Gly Gln Arg Leu Leu Gln Lys Leu Gly Ala Asp Leu 
225                 230                 235                 240 


Ile Asp Met Asp Gln Ile Gln Val His Pro Thr Gly Phe Ile Asp Pro 
                245                 250                 255     


Asn Asp Arg Asn Ser Ser Trp Lys Phe Leu Ala Ala Glu Ser Leu Arg 
            260                 265                 270         


Gly Leu Gly Gly Ile Leu Leu Asn Pro Ile Thr Gly Arg Arg Phe Val 
        275                 280                 285             


Asn Glu Leu Thr Thr Arg Asp Val Val Thr Glu Ala Ile Gln Lys His 
    290                 295                 300                 


Cys Pro Gln Asp Asp Asn Arg Ala Leu Leu Val Met Ser Glu Lys Met 
305                 310                 315                 320 


Tyr Thr Asp Leu Lys Asn Asn Leu Asp Phe Tyr Met Phe Lys Lys Leu 
                325                 330                 335     


Val Gln Lys Leu Ser Leu Ser Gln Val Val Ser Glu Tyr Lys Leu Pro 
            340                 345                 350         


Ile Thr Val Ser Gln Leu Cys Gln Glu Leu Gln Thr Tyr Ser Ser Phe 
        355                 360                 365             


Thr Ser Lys Ala Asp Pro Leu Gly Arg Thr Val Val Leu Asn Glu Phe 
    370                 375                 380                 


Gly Ala Asp Ile Thr Pro Glu Thr Met Val Phe Ile Gly Glu Val Thr 
385                 390                 395                 400 


Pro Val Val His Phe Thr Met Gly Gly Ala Arg Ile Asn Val Lys Ala 
                405                 410                 415     


Gln Val Ile Gly Lys Asn Asp Glu Pro Leu Leu Asn Gly Leu Tyr Ala 
            420                 425                 430         


Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu Gly Gly 
        435                 440                 445             


Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Thr Ala Ala Glu Ser 
    450                 455                 460                 


Ile Ala Asn Asn His Lys 
465                 470 


<210>  110
<211>  470
<212>  PRT
<213>  Kluyvermyces polysporus

<400>  110

Met Ser Thr Lys Lys Pro Val Val Ile Ile Gly Thr Gly Leu Ala Gly 
1               5                   10                  15      


Leu Ser Ala Gly Asn Gln Leu Val Asn Met His Lys Val Pro Ile Ile 
            20                  25                  30          


Met Leu Asp Lys Ala Ser Ser Ile Gly Gly Asn Ser Thr Lys Ala Ser 
        35                  40                  45              


Ser Gly Ile Asn Gly Ala Ser Thr Ile Thr Gln Gln Gln Leu Asn Val 
    50                  55                  60                  


Lys Asp Ser Pro Asp Leu Phe Leu Gln Asp Thr Val Lys Ser Ala Lys 
65                  70                  75                  80  


Gly Arg Gly Ile Glu Ser Leu Met Lys Lys Leu Ser Gln Asp Ser Asn 
                85                  90                  95      


Ser Ala Ile His Trp Leu Gln Gln Asp Phe Asp Leu Lys Leu Asp Leu 
            100                 105                 110         


Leu Ala Gln Leu Gly Gly His Ser Val Pro Arg Thr His Arg Ser Ser 
        115                 120                 125             


Gly Lys Leu Pro Pro Gly Phe Glu Ile Val Gln Ala Leu Ser Asn Lys 
    130                 135                 140                 


Leu Lys Ala Ile Ser Glu Ser Asp Pro Glu Phe Val Arg Ile Leu Leu 
145                 150                 155                 160 


Asn Ser Lys Val Val Asp Val Ser Val Asn Asn Glu Gly Lys Val Glu 
                165                 170                 175     


Ser Ile Asp Tyr Val Asp Ala Glu Gly Lys His His Lys Ile Ala Thr 
            180                 185                 190         


Asp Asn Val Val Phe Cys Ser Gly Gly Phe Gly His Ser Ala Glu Met 
        195                 200                 205             


Leu Asn Lys Tyr Ala Pro Glu Leu Ala Asn Leu Pro Thr Thr Asn Gly 
    210                 215                 220                 


Gln Gln Thr Thr Gly Asp Gly Gln Arg Ile Leu Glu Lys Leu Gly Ala 
225                 230                 235                 240 


Asp Leu Ile Asp Met Ser Gln Ile Gln Val His Pro Thr Gly Phe Ile 
                245                 250                 255     


Asp Pro Ala Asn Arg Asp Ser Lys Trp Lys Phe Leu Ala Ala Glu Ala 
            260                 265                 270         


Leu Arg Gly Leu Gly Gly Ile Leu Leu Asn Pro Ser Thr Gly Lys Arg 
        275                 280                 285             


Phe Val Asn Glu Leu Thr Thr Arg Asp Leu Val Thr Glu Ala Ile Gln 
    290                 295                 300                 


Ser Gln Cys Pro Arg Asp Asp Asn Lys Ala Phe Leu Val Met Ser Glu 
305                 310                 315                 320 


Lys Val Tyr Glu Asn Tyr Lys Asn Asn Met Asp Phe Tyr Leu Phe Lys 
                325                 330                 335     


Lys Leu Val Ser Lys Met Thr Ile Lys Glu Phe Val Glu Thr Tyr Lys 
            340                 345                 350         


Leu Pro Ile Ser Ala Asp Ala Val Thr Gln Asp Leu Ile Asp Tyr Ser 
        355                 360                 365             


Val Asp Lys Thr Asp Lys Phe Gly Arg Pro Leu Val Ile Asn Val Phe 
    370                 375                 380                 


Asp Glu Lys Leu Thr Glu Asp Ser Glu Ile Tyr Val Gly Glu Val Thr 
385                 390                 395                 400 


Pro Val Val His Phe Thr Met Gly Gly Ala Lys Ile Asn Thr Glu Ser 
                405                 410                 415     


Gln Val Ile Asn Lys Asn Gly Gln Val Leu Ala Lys Gly Ile Tyr Ala 
            420                 425                 430         


Ala Gly Glu Val Ser Gly Gly Val His Gly Ser Asn Arg Leu Gly Gly 
        435                 440                 445             


Ser Ser Leu Leu Glu Cys Val Val Tyr Gly Arg Ser Ala Ala Asp Asn 
    450                 455                 460                 


Ile Ala Lys Asn Ile Glu 
465                 470 


<210>  111
<211>  487
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  111

Met Leu His Arg Tyr Ile Arg Leu Phe Ser Phe Cys Val Ile Leu Tyr 
1               5                   10                  15      


Leu Val Tyr Leu Leu Leu Thr Lys Glu Ser Asn Val Met Ser Lys Pro 
            20                  25                  30          


Val Val Val Ile Gly Ser Gly Leu Ala Gly Leu Thr Thr Ser Ser Gln 
        35                  40                  45              


Leu Ala Lys Phe Asn Ile Pro Ile Val Leu Leu Glu Lys Thr Ser Ser 
    50                  55                  60                  


Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser Gly Ile Asn Gly Ala Gly 
65                  70                  75                  80  


Thr Glu Thr Gln Ser Arg Leu His Val Glu Asp His Pro Glu Leu Phe 
                85                  90                  95      


Ala Asp Asp Thr Ile Lys Ser Ala Lys Gly Lys Gly Val Val Ala Leu 
            100                 105                 110         


Met Glu Lys Leu Ser Lys Asp Ser Ser Asp Ala Ile Ser Trp Leu Gln 
        115                 120                 125             


Asn Asp Phe Lys Ile Pro Leu Asp Lys Leu Ala Gln Leu Gly Gly His 
    130                 135                 140                 


Ser Val Pro Arg Thr His Arg Ser Ser Gly Lys Leu Pro Pro Gly Phe 
145                 150                 155                 160 


Gln Ile Val Asp Thr Leu Lys Lys Ala Leu Glu Ser Tyr Asp Ser Lys 
                165                 170                 175     


Ala Val Lys Ile Gln Leu Asn Ser Lys Val Val Asp Val Lys Leu Asp 
            180                 185                 190         


Ser Asn Asn Arg Val Ser Ser Val Val Phe Glu Asp Gln Asp Gly Thr 
        195                 200                 205             


His Thr Ile Glu Thr Asn Asn Val Val Phe Cys Thr Gly Gly Phe Gly 
    210                 215                 220                 


Phe Asn Lys Lys Leu Leu Glu Lys Tyr Ala Pro His Leu Val Asp Leu 
225                 230                 235                 240 


Pro Thr Thr Asn Gly Glu Gln Thr Leu Gly Glu Gly Gln Val Leu Leu 
                245                 250                 255     


Glu Lys Leu Gly Ala Lys Leu Ile Asp Met Asp Gln Ile Gln Val His 
            260                 265                 270         


Pro Thr Gly Phe Ile Asp Pro Ala Asn Pro Asp Ser Asn Trp Lys Phe 
        275                 280                 285             


Leu Ala Ala Glu Ala Leu Arg Gly Leu Gly Gly Val Leu Ile Asn Pro 
    290                 295                 300                 


His Thr Gly Gln Arg Phe Val Asn Glu Leu Thr Thr Arg Asp Met Val 
305                 310                 315                 320 


Thr Glu Ala Ile Gln Ser Lys Ser Glu Ser Lys Thr Ala Tyr Leu Val 
                325                 330                 335     


Met Ser Glu Ser Leu Tyr Glu Asn Tyr Lys Pro Asn Met Asp Phe Tyr 
            340                 345                 350         


Met Phe Lys Lys Leu Val Ser Lys Lys Thr Val Ala Glu Phe Ala Glu 
        355                 360                 365             


Asp Leu Pro Val Ser Val Asp Gln Leu Ile Ala Glu Leu Ser Thr Tyr 
    370                 375                 380                 


Ser Asp Leu Ser Lys Asp Asp His Leu Gly Arg Lys Phe Arg Glu Asn 
385                 390                 395                 400 


Thr Phe Gly Ser Ser Leu Ser Ser Asp Ser Thr Ile Phe Val Gly Lys 
                405                 410                 415     


Ile Thr Pro Val Val His Phe Thr Met Gly Gly Ala Lys Ile Asp Glu 
            420                 425                 430         


Gln Ala Arg Val Leu Asn Ala Glu Gly Lys Pro Leu Ala Thr Gly Ile 
        435                 440                 445             


Tyr Ala Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu 
    450                 455                 460                 


Gly Gly Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Gln Ala Ala 
465                 470                 475                 480 


Lys Ser Ile Arg Ala Asn Leu 
                485         


<210>  112
<211>  1139
<212>  PRT
<213>  Trypanosoma brucei

<400>  112

Met Val Asp Gly Arg Ser Ser Ala Ser Ile Val Ala Val Asp Pro Glu 
1               5                   10                  15      


Arg Ala Ala Arg Glu Arg Asp Ala Ala Ala Arg Ala Leu Leu Gln Asp 
            20                  25                  30          


Ser Pro Leu His Thr Thr Met Gln Tyr Ala Thr Ser Gly Leu Glu Leu 
        35                  40                  45              


Thr Val Pro Tyr Ala Leu Lys Val Val Ala Ser Ala Asp Thr Phe Asp 
    50                  55                  60                  


Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Cys Ala Trp Gln Leu 
65                  70                  75                  80  


Ala Asp Thr Val Leu Asn Ser Phe Asn Pro Asn Ser Glu Val Ser Leu 
                85                  90                  95      


Val Gly Arg Leu Pro Val Gly Gln Lys His Gln Met Ser Ala Pro Leu 
            100                 105                 110         


Lys Arg Val Met Ala Cys Cys Gln Arg Val Tyr Asn Ser Ser Ala Gly 
        115                 120                 125             


Cys Phe Asp Pro Ser Thr Ala Pro Val Ala Lys Ala Leu Arg Glu Ile 
    130                 135                 140                 


Ala Leu Gly Lys Glu Arg Asn Asn Ala Cys Leu Glu Ala Leu Thr Gln 
145                 150                 155                 160 


Ala Cys Thr Leu Pro Asn Ser Phe Val Ile Asp Phe Glu Ala Gly Thr 
                165                 170                 175     


Ile Ser Arg Lys His Glu His Ala Ser Leu Asp Leu Gly Gly Val Ser 
            180                 185                 190         


Lys Gly Tyr Ile Val Asp Tyr Val Ile Asp Asn Ile Asn Ala Ala Gly 
        195                 200                 205             


Phe Gln Asn Val Phe Phe Asp Trp Gly Gly Asp Cys Arg Ala Ser Gly 
    210                 215                 220                 


Met Asn Ala Arg Asn Thr Pro Trp Val Val Gly Ile Thr Arg Pro Pro 
225                 230                 235                 240 


Ser Leu Asp Met Leu Pro Asn Pro Pro Lys Glu Ala Ser Tyr Ile Ser 
                245                 250                 255     


Val Ile Ser Leu Asp Asn Glu Ala Leu Ala Thr Ser Gly Asp Tyr Glu 
            260                 265                 270         


Asn Leu Ile Tyr Thr Ala Asp Asp Lys Pro Leu Thr Cys Thr Tyr Asp 
        275                 280                 285             


Trp Lys Gly Lys Glu Leu Met Lys Pro Ser Gln Ser Asn Ile Ala Gln 
    290                 295                 300                 


Val Ser Val Lys Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala Thr 
305                 310                 315                 320 


Ala Cys Phe Ile Lys Arg Asp Pro Ala Lys Val Arg Gln Leu Leu Asp 
                325                 330                 335     


Gly Trp Arg Tyr Val Arg Asp Thr Val Arg Asp Tyr Arg Val Tyr Val 
            340                 345                 350         


Arg Glu Asn Glu Arg Val Ala Lys Met Phe Glu Ile Ala Thr Glu Asp 
        355                 360                 365             


Ala Glu Met Arg Lys Arg Arg Ile Ser Asn Thr Leu Pro Ala Arg Val 
    370                 375                 380                 


Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu Ala 
385                 390                 395                 400 


Ala Gly Cys Gly Ala Gln Val Val Leu Met Glu Lys Glu Ala Lys Leu 
                405                 410                 415     


Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly Thr 
            420                 425                 430         


Arg Ala Gln Ala Lys Ala Ser Ile Val Asp Gly Gly Lys Tyr Phe Glu 
        435                 440                 445             


Arg Asp Thr Tyr Lys Ser Gly Ile Gly Gly Asn Thr Asp Pro Ala Leu 
    450                 455                 460                 


Val Lys Thr Leu Ser Met Lys Ser Ala Asp Ala Ile Gly Trp Leu Thr 
465                 470                 475                 480 


Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His Ser 
                485                 490                 495     


Arg Lys Arg Thr His Arg Ala Pro Asp Lys Lys Asp Gly Thr Pro Leu 
            500                 505                 510         


Pro Ile Gly Phe Thr Ile Met Lys Thr Leu Glu Asp His Val Arg Gly 
        515                 520                 525             


Asn Leu Ser Gly Arg Ile Thr Ile Met Glu Asn Cys Ser Val Thr Ser 
    530                 535                 540                 


Leu Leu Ser Glu Thr Lys Glu Arg Pro Asp Gly Thr Lys Gln Ile Arg 
545                 550                 555                 560 


Val Thr Gly Val Glu Phe Thr Gln Ala Gly Ser Gly Lys Thr Thr Ile 
                565                 570                 575     


Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp Lys 
            580                 585                 590         


Thr Ala Asp Ser Leu Leu Arg Glu His Ala Pro His Leu Val Asn Phe 
        595                 600                 605             


Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu Ala 
    610                 615                 620                 


Gln Arg Leu Gly Ala Gln Leu Val Asp Met Asp Lys Val Gln Leu His 
625                 630                 635                 640 


Pro Thr Gly Leu Ile Asn Pro Lys Asp Pro Ala Asn Pro Thr Lys Phe 
                645                 650                 655     


Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn Lys 
            660                 665                 670         


Gln Gly Lys Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val Ser 
        675                 680                 685             


Lys Ala Ile Met Glu Gln Gly Ala Glu Tyr Pro Gly Ser Gly Gly Ser 
    690                 695                 700                 


Met Phe Ala Tyr Cys Val Leu Asn Ala Ala Ala Gln Lys Leu Phe Gly 
705                 710                 715                 720 


Val Ser Ser His Glu Phe Tyr Trp Lys Lys Met Gly Leu Phe Val Lys 
                725                 730                 735     


Ala Asp Thr Met Arg Asp Leu Ala Ala Leu Ile Gly Cys Pro Val Glu 
            740                 745                 750         


Ser Val Gln Gln Thr Leu Glu Glu Tyr Glu Arg Leu Ser Ile Ser Gln 
        755                 760                 765             


Arg Ser Cys Pro Ile Thr Arg Lys Ser Val Tyr Pro Cys Val Leu Gly 
    770                 775                 780                 


Thr Lys Gly Pro Tyr Tyr Val Ala Phe Val Thr Pro Ser Ile His Tyr 
785                 790                 795                 800 


Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Ile Gln Met Lys 
                805                 810                 815     


Asn Thr Ser Ser Arg Ala Pro Leu Ser His Ser Asn Pro Ile Leu Gly 
            820                 825                 830         


Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg 
        835                 840                 845             


Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala 
    850                 855                 860                 


Gly Asp Arg Ala Ser Thr Ile Leu Gln Arg Lys Ser Ser Ala Leu Ser 
865                 870                 875                 880 


Phe Lys Val Trp Thr Thr Val Val Leu Arg Glu Val Arg Glu Gly Gly 
                885                 890                 895     


Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala 
            900                 905                 910         


Leu Gln Arg Ser Gly Leu Ser Leu Gly Gln Phe Ile Ala Ile Arg Gly 
        915                 920                 925             


Asp Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu 
    930                 935                 940                 


Pro Asp Asp Leu Gly Met Ile Asp Ile Leu Ala Arg Ser Asp Lys Gly 
945                 950                 955                 960 


Thr Leu Arg Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu 
                965                 970                 975     


Met Lys Ala Cys Gly Gly Leu Val Ile Glu Arg Arg Leu Ser Asp Lys 
            980                 985                 990         


His Phe Val Phe Met Gly His Ile  Ile Asn Lys Leu Cys  Leu Ile Ala 
        995                 1000                 1005             


Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln Ile Ile  Lys Ala Ala 
    1010                 1015                 1020             


Phe Met  Lys Pro Phe Ile Asp  Thr Leu Glu Ser Val  His Leu Ile 
    1025                 1030                 1035             


Tyr Ala  Ala Glu Asp Val Thr  Glu Leu Thr Tyr Arg  Glu Val Leu 
    1040                 1045                 1050             


Glu Glu  Arg Arg Arg Glu Ser  Arg Gly Lys Phe Lys  Lys Thr Phe 
    1055                 1060                 1065             


Val Leu  Asn Arg Pro Pro Pro  Leu Trp Thr Asp Gly  Val Gly Phe 
    1070                 1075                 1080             


Ile Asp  Arg Gly Ile Leu Thr  Asn His Val Gln Pro  Pro Ser Asp 
    1085                 1090                 1095             


Asn Leu  Leu Val Ala Ile Cys  Gly Pro Pro Val Met  Gln Arg Ile 
    1100                 1105                 1110             


Val Lys  Ala Thr Leu Lys Thr  Leu Gly Tyr Asn Met  Asn Leu Val 
    1115                 1120                 1125             


Arg Thr  Val Asp Glu Thr Glu  Pro Ser Gly Ser 
    1130                 1135                 


<210>  113
<211>  1139
<212>  PRT
<213>  Trypanosoma cruzi

<400>  113

Met Ala Asp Gly Arg Ser Ser Ala Ser Val Val Ala Val Asp Pro Glu 
1               5                   10                  15      


Lys Ala Ala Arg Glu Arg Asp Glu Ala Ala Arg Ala Leu Leu Arg Asp 
            20                  25                  30          


Ser Pro Leu Gln Thr His Leu Gln Tyr Met Thr Asn Gly Leu Glu Leu 
        35                  40                  45              


Thr Val Pro Phe Thr Leu Lys Val Val Ala Glu Ala Val Ala Phe Ser 
    50                  55                  60                  


Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Ser Ala Trp His Leu 
65                  70                  75                  80  


Ala Asp Thr Val Leu Asn Asn Phe Asn Pro Asn Ser Glu Ile Ser Met 
                85                  90                  95      


Ile Gly Arg Leu Pro Val Gly Gln Lys His Thr Met Ser Ala Thr Leu 
            100                 105                 110         


Lys Ser Val Ile Thr Cys Cys Gln His Val Phe Asn Ser Ser Arg Gly 
        115                 120                 125             


Val Phe Asp Pro Ala Thr Gly Pro Ile Ile Glu Ala Leu Arg Ala Lys 
    130                 135                 140                 


Val Ala Glu Lys Ala Ser Val Ser Asp Glu Gln Met Glu Lys Leu Phe 
145                 150                 155                 160 


Arg Val Cys Asn Phe Ser Ser Ser Phe Ile Val Asp Leu Glu Met Gly 
                165                 170                 175     


Thr Ile Ala Arg Lys His Glu Asp Ala Arg Phe Asp Leu Gly Gly Val 
            180                 185                 190         


Ser Lys Gly Tyr Ile Val Asp Tyr Val Val Glu Arg Leu Asn Ala Ala 
        195                 200                 205             


Gly Ile Val Asp Val Tyr Phe Glu Trp Gly Gly Asp Cys Arg Ala Ser 
    210                 215                 220                 


Gly Thr Asn Ala Arg Arg Thr Pro Trp Met Val Gly Ile Ile Arg Pro 
225                 230                 235                 240 


Pro Ser Leu Glu Gln Leu Arg Asn Pro Pro Lys Asp Pro Ser Tyr Ile 
                245                 250                 255     


Arg Val Leu Pro Leu Asn Asp Glu Ala Leu Cys Thr Ser Gly Asp Tyr 
            260                 265                 270         


Glu Asn Leu Thr Glu Gly Ser Asn Lys Lys Leu Tyr Thr Ser Ile Phe 
        275                 280                 285             


Asp Trp Lys Lys Arg Ser Leu Leu Glu Pro Val Glu Ser Glu Leu Ala 
    290                 295                 300                 


Gln Val Ser Ile Arg Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala 
305                 310                 315                 320 


Thr Ala Ser Leu Ile Lys Arg Asp Ile Lys Lys Val Arg Gln Met Leu 
                325                 330                 335     


Glu Asp Trp Arg His Val Arg Asn Arg Val Thr Asn Tyr Val Thr Tyr 
            340                 345                 350         


Thr Arg Gln Gly Glu Arg Val Ala Arg Met Phe Glu Ile Ala Thr Asp 
        355                 360                 365             


Asn Ala Glu Ile Arg Lys Lys Arg Ile Ala Gly Ser Leu Pro Ala Arg 
    370                 375                 380                 


Val Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu 
385                 390                 395                 400 


Ala Thr Ala Cys Gly Ala Gln Val Ile Leu Leu Glu Lys Glu Pro Lys 
                405                 410                 415     


Val Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly 
            420                 425                 430         


Thr Arg Ala Gln Ala Glu Gln Asp Val Tyr Asp Ser Gly Lys Tyr Phe 
        435                 440                 445             


Glu Arg Asp Thr His Lys Ser Gly Leu Gly Gly Ser Thr Asp Pro Gly 
    450                 455                 460                 


Leu Val Arg Thr Leu Ser Val Lys Ser Gly Asp Ala Ile Ser Trp Leu 
465                 470                 475                 480 


Ser Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His 
                485                 490                 495     


Ser Arg Lys Arg Thr His Arg Ala Pro Asp Lys Ala Asp Gly Thr Pro 
            500                 505                 510         


Val Pro Ile Gly Phe Thr Ile Met Gln Thr Leu Glu Gln His Val Arg 
        515                 520                 525             


Thr Lys Leu Ala Asp Arg Val Thr Ile Met Glu Asn Thr Thr Val Thr 
    530                 535                 540                 


Ser Leu Leu Ser Lys Ser Arg Val Arg His Asp Gly Ala Lys Gln Val 
545                 550                 555                 560 


Arg Val Tyr Gly Val Glu Val Leu Gln Asp Glu Gly Val Val Ser Arg 
                565                 570                 575     


Ile Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp 
            580                 585                 590         


Lys Thr Pro Asn Ser Leu Leu Gln Glu Phe Ala Pro Gln Leu Ser Gly 
        595                 600                 605             


Phe Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu 
    610                 615                 620                 


Ala Arg Glu Leu Gly Val Lys Leu Val Asp Met Asp Lys Val Gln Leu 
625                 630                 635                 640 


His Pro Thr Gly Leu Ile Asp Pro Lys Asp Pro Ala Asn Pro Thr Lys 
                645                 650                 655     


Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn 
            660                 665                 670         


Lys Lys Gly Glu Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val 
        675                 680                 685             


Ser Asn Ala Ile Ile Glu Gln Gly Asp Glu Tyr Pro Asp Ala Gly Gly 
    690                 695                 700                 


Ser Lys Phe Ala Phe Cys Val Leu Asn Asp Ala Ala Val Lys Leu Phe 
705                 710                 715                 720 


Gly Val Asn Ser His Gly Phe Tyr Trp Lys Arg Leu Gly Leu Phe Val 
                725                 730                 735     


Lys Ala Asp Thr Val Glu Lys Leu Ala Ala Leu Ile Gly Cys Pro Val 
            740                 745                 750         


Glu Asn Val Arg Asn Thr Leu Gly Asp Tyr Glu Gln Leu Ser Lys Glu 
        755                 760                 765             


Asn Arg Gln Cys Pro Lys Thr Arg Lys Val Val Tyr Pro Cys Val Val 
    770                 775                 780                 


Gly Pro Gln Gly Pro Phe Tyr Val Ala Phe Val Thr Pro Ser Ile His 
785                 790                 795                 800 


Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Met Gln Leu 
                805                 810                 815     


Glu Glu Asn Thr Thr Ser Pro Phe Gly His Arg Arg Pro Ile Phe Gly 
            820                 825                 830         


Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg 
        835                 840                 845             


Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala 
    850                 855                 860                 


Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys Pro Val Pro Leu Ser 
865                 870                 875                 880 


Phe Lys Thr Trp Thr Thr Val Ile Leu Arg Glu Val Arg Glu Gly Gly 
                885                 890                 895     


Met Tyr Gly Thr Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala 
            900                 905                 910         


Leu Gln Arg Ser Gly Leu Gln Leu Gly Gln Phe Ile Ala Ile Arg Gly 
        915                 920                 925             


Glu Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu 
    930                 935                 940                 


Pro Asp Asp Leu Gly Val Ile Gly Ile Leu Ala Arg Ser Asp Lys Gly 
945                 950                 955                 960 


Thr Leu Lys Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu 
                965                 970                 975     


Met Lys Gly Cys Gly Gly Leu Val Ile Glu Arg Arg Phe Ser Glu Arg 
            980                 985                 990         


Tyr Leu Tyr Phe Ser Gly His Ala  Leu Lys Lys Leu Cys  Leu Ile Ala 
        995                 1000                 1005             


Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln Ile Ile  Arg Ala Ala 
    1010                 1015                 1020             


Leu Lys  Lys Pro Phe Leu Glu  Asn Ile Glu Ser Ile  Arg Leu Ile 
    1025                 1030                 1035             


Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr Tyr Arg  Glu Leu Leu 
    1040                 1045                 1050             


Glu His  His Gln Arg Asp Ser  Lys Gly Lys Phe Arg  Ser Ile Phe 
    1055                 1060                 1065             


Val Leu  Asn Arg Pro Pro Pro  Ile Trp Thr Asp Gly  Val Gly Phe 
    1070                 1075                 1080             


Ile Asp  Lys Lys Leu Leu Ser  Ser Ser Val Gln Pro  Pro Ala Lys 
    1085                 1090                 1095             


Asp Leu  Leu Val Ala Ile Cys  Gly Pro Pro Ile Met  Gln Arg Val 
    1100                 1105                 1110             


Val Lys  Thr Cys Leu Lys Ser  Leu Gly Tyr Asp Met  Gln Leu Val 
    1115                 1120                 1125             


Arg Thr  Val Asp Glu Val Glu  Thr Gln Asn Ser 
    1130                 1135                 


<210>  114
<211>  1144
<212>  PRT
<213>  Leishmania braziliensis

<400>  114

Met Ala Asp Gly Lys Thr Ser Ala Ser Val Val Ala Val Asp Pro Glu 
1               5                   10                  15      


Arg Ala Ala Lys Glu Arg Asp Ala Ala Ala Arg Ala Met Leu Gln Asp 
            20                  25                  30          


Gly Gly Val Ser Pro Val Gly Lys Ala Gln Leu Leu Lys Lys Gly Leu 
        35                  40                  45              


Ala Tyr Ala Val Pro Tyr Thr Leu Lys Ile Val Val Ala Asp Pro Lys 
    50                  55                  60                  


Ala Met Glu Lys Thr Thr Ala Asp Val Glu Lys Val Leu Gln Thr Ala 
65                  70                  75                  80  


Phe Gln Val Val Asp Thr Leu Leu Asn Asn Phe Asn Glu Asn Ser Glu 
                85                  90                  95      


Val Ser Arg Ile Asn Arg Met Pro Val Gly Glu Glu His Gln Met Ser 
            100                 105                 110         


Ala Ala Leu Lys Arg Val Met Gly Cys Cys Gln Arg Val Tyr Asn Ser 
        115                 120                 125             


Ser Arg Gly Ala Phe Asp Pro Ala Val Gly Pro Leu Val Arg Glu Leu 
    130                 135                 140                 


Arg Glu Ala Ala Arg Glu Gly Arg Thr Leu Pro Ala Glu Arg Ile Asn 
145                 150                 155                 160 


Ala Leu Leu Ser Lys Cys Thr Leu Asn Ile Ser Phe Ser Ile Asp Leu 
                165                 170                 175     


Asn Arg Gly Thr Ile Ala Arg Lys His Ala Asp Ala Met Leu Asp Leu 
            180                 185                 190         


Gly Gly Val Asn Lys Gly Tyr Gly Val Asp Tyr Val Val Glu His Leu 
        195                 200                 205             


Asn Asn Leu Gly Tyr Asp Asp Val Phe Phe Glu Trp Gly Gly Asp Val 
    210                 215                 220                 


Arg Ala Ser Gly Lys Asn Pro Ser Asn Gln His Trp Val Val Gly Ile 
225                 230                 235                 240 


Ala Arg Pro Pro Ala Leu Ala Asp Ile Arg Thr Val Val Pro Gln Asp 
                245                 250                 255     


Lys Gln Ser Phe Ile Arg Val Val Cys Leu Asn Asp Glu Ala Ile Ala 
            260                 265                 270         


Thr Ser Gly Asp Tyr Glu Asn Leu Val Glu Gly Pro Gly Ser Lys Val 
        275                 280                 285             


Tyr Ser Ser Thr Phe Asn Ala Thr Ser Lys Ser Leu Leu Glu Pro Thr 
    290                 295                 300                 


Glu Thr Asn Ile Ala Gln Val Ser Val Lys Cys Tyr Ser Cys Met Tyr 
305                 310                 315                 320 


Ala Asp Ala Leu Ala Thr Ala Ala Leu Leu Lys Asn Asn Pro Thr Ala 
                325                 330                 335     


Val Arg Arg Met Leu Asp Asn Trp Arg Tyr Val Arg Asp Thr Val Thr 
            340                 345                 350         


Asp Tyr Thr Thr Tyr Ser Arg Glu Gly Glu Arg Val Ala Lys Met Phe 
        355                 360                 365             


Glu Ile Ala Thr Glu Asp Lys Glu Met Arg Ala Lys Arg Ile Ser Gly 
    370                 375                 380                 


Ser Leu Pro Ala Arg Val Ile Ile Val Gly Gly Gly Leu Ala Gly Cys 
385                 390                 395                 400 


Ser Ala Ala Ile Glu Ala Val Asn Cys Gly Ala Gln Val Ile Leu Leu 
                405                 410                 415     


Glu Lys Glu Ala Lys Ile Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly 
            420                 425                 430         


Ile Asn Ala Trp Gly Thr Arg Ala Gln Ala Lys Gln Gly Val Met Asp 
        435                 440                 445             


Gly Gly Lys Phe Phe Glu Arg Asp Thr His Arg Ser Gly Lys Gly Gly 
    450                 455                 460                 


His Cys Asp Pro Cys Leu Val Lys Thr Leu Ser Val Lys Ser Ser Asp 
465                 470                 475                 480 


Ala Val Lys Trp Leu Ser Glu Leu Gly Val Pro Leu Thr Val Leu Ser 
                485                 490                 495     


Gln Leu Gly Gly Ala Ser Arg Lys Arg Cys His Arg Ala Pro Asp Lys 
            500                 505                 510         


Ser Asp Gly Thr Pro Val Pro Ile Gly Phe Thr Ile Met Lys Thr Leu 
        515                 520                 525             


Glu Asn His Ile Ile Asn Asp Leu Ser His Gln Val Thr Val Met Thr 
    530                 535                 540                 


Gly Ile Lys Val Thr Gly Leu Glu Ser Thr Ser His Ala Arg Pro Asp 
545                 550                 555                 560 


Gly Val Leu Val Lys His Val Thr Gly Val Arg Leu Ile Gln Gly Asp 
                565                 570                 575     


Gly Gln Ser Arg Val Leu Asn Ala Asp Ala Val Ile Leu Ala Thr Gly 
            580                 585                 590         


Gly Phe Ser Asn Asp His Thr Ala Asn Ser Leu Leu Gln Gln Tyr Ala 
        595                 600                 605             


Pro Gln Leu Ser Ser Phe Pro Thr Thr Asn Gly Val Trp Ala Thr Gly 
    610                 615                 620                 


Asp Gly Val Lys Ala Ala Arg Glu Leu Gly Val Glu Leu Val Asp Met 
625                 630                 635                 640 


Asp Lys Val Gln Leu His Pro Thr Gly Leu Leu Asp Pro Lys Asp Pro 
                645                 650                 655     


Ser Asn Arg Thr Lys Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly 
            660                 665                 670         


Gly Val Leu Leu Asn Lys Asn Gly Glu Arg Phe Val Asn Glu Leu Asp 
        675                 680                 685             


Leu Arg Ser Val Val Ser Gln Ala Ile Ile Glu Gln Asn Asn Val Tyr 
    690                 695                 700                 


Pro Gly Ser Gly Gly Ser Lys Phe Ala Tyr Cys Val Leu Asn Glu Ala 
705                 710                 715                 720 


Ala Ala Lys Leu Phe Gly Lys Asn Phe Leu Gly Phe Tyr Trp His Arg 
                725                 730                 735     


Leu Gly Leu Phe Glu Lys Val Glu Asp Val Ala Gly Leu Ala Lys Leu 
            740                 745                 750         


Ile Gly Cys Pro Glu Glu Asn Val Thr Ala Thr Leu Lys Glu Tyr Lys 
        755                 760                 765             


Glu Leu Ser Ser Lys Lys Leu His Ala Cys Pro Leu Thr Asn Lys Asn 
    770                 775                 780                 


Val Phe Pro Cys Thr Leu Gly Thr Glu Gly Pro Tyr Tyr Val Ala Phe 
785                 790                 795                 800 


Val Thr Pro Ser Ile His Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro 
                805                 810                 815     


Ser Ala Glu Met Gln Thr Ile Asp Asn Thr Gly Val Thr Pro Val Arg 
            820                 825                 830         


Arg Pro Ile Leu Gly Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val 
        835                 840                 845             


His Gly Gly Asn Arg Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val 
    850                 855                 860                 


Phe Gly Arg Ile Ala Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys 
865                 870                 875                 880 


Asn Ala Gly Leu Ser Met Thr Glu Trp Ser Thr Val Val Leu Arg Glu 
                885                 890                 895     


Val Arg Glu Gly Gly Val Tyr Gly Thr Gly Ser Arg Val Leu Arg Phe 
            900                 905                 910         


Asn Met Pro Gly Ala Leu Gln Lys Thr Gly Leu Ala Leu Gly Gln Phe 
        915                 920                 925             


Ile Ala Met Arg Gly Asp Trp Asp Gly Gln Gln Leu Leu Gly Tyr Tyr 
    930                 935                 940                 


Ser Pro Ile Thr Leu Pro Asp Asp Ile Gly Val Ile Gly Ile Leu Ala 
945                 950                 955                 960 


Arg Ala Asp Lys Gly Arg Leu Ala Glu Trp Ile Ser Ala Leu Gln Pro 
                965                 970                 975     


Gly Asp Ala Val Glu Met Lys Ala Cys Gly Gly Leu Ile Ile His Arg 
            980                 985                 990         


Arg Phe Ala Ala Arg His Leu Phe  Phe Arg Ser His Lys  Ile Arg Lys 
        995                 1000                 1005             


Leu Ala  Leu Ile Gly Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln 
    1010                 1015                 1020             


Ile Val  Arg Ala Ala Val Lys  Lys Pro Phe Val Asp  Ser Ile Glu 
    1025                 1030                 1035             


Ser Ile  Gln Phe Ile Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr 
    1040                 1045                 1050             


Tyr Arg  Thr Leu Leu Glu Ser  Tyr Glu Lys Glu Tyr  Gly Ser Gly 
    1055                 1060                 1065             


Lys Phe  Lys Cys His Phe Val  Leu Asn Asn Pro Pro  Ser Gln Trp 
    1070                 1075                 1080             


Thr Glu  Gly Val Gly Phe Val  Asp Thr Ala Leu Leu  Arg Ser Ala 
    1085                 1090                 1095             


Val Gln  Ala Pro Ser Asn Asp  Leu Leu Val Ala Ile  Cys Gly Pro 
    1100                 1105                 1110             


Pro Ile  Met Gln Arg Ala Val  Lys Ser Ala Leu Lys  Gly Leu Gly 
    1115                 1120                 1125             


Tyr Asn  Met Asn Leu Val Arg  Thr Val Asp Glu Pro  Glu Pro Leu 
    1130                 1135                 1140             


Ser 
    


<210>  115
<211>  880
<212>  PRT
<213>  Mannheimia succiiniproducens

<400>  115

Met Thr Glu Glu Tyr Leu Met Met Arg Asn Asn Ile Asn Met Leu Gly 
1               5                   10                  15      


Arg Phe Leu Gly Glu Thr Ile Gln Glu Ala Gln Gly Asp Asp Ile Leu 
            20                  25                  30          


Glu Leu Ile Glu Asn Ile Arg Val Leu Ser Arg Asn Ser Arg Ser Gly 
        35                  40                  45              


Asp Asp Lys Ala Arg Ala Ala Leu Leu Asp Thr Leu Ser Thr Ile Ser 
    50                  55                  60                  


Ala Asp Asn Ile Ile Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn 
65                  70                  75                  80  


Leu Thr Asn Val Ala Glu Gln Tyr Gln Thr Met Ser Arg Ser His Glu 
                85                  90                  95      


Asp Lys Val Ser Ala Glu Arg Ser Thr Ala Ala Leu Phe Ala Arg Leu 
            100                 105                 110         


Lys Glu Gln His Val Ser Gln Glu Glu Ile Ile Lys Thr Val Gln Lys 
        115                 120                 125             


Leu Leu Ile Glu Ile Val Leu Thr Ala His Pro Thr Glu Val Thr Arg 
    130                 135                 140                 


Arg Ser Leu Met His Lys Gln Val Glu Ile Asn Lys Cys Leu Ala Gln 
145                 150                 155                 160 


Leu Asp His Thr Asp Leu Thr Ala Glu Glu Gln Lys Asn Ile Glu Tyr 
                165                 170                 175     


Lys Leu Leu Arg Leu Ile Ala Glu Ala Trp His Thr Asn Glu Ile Arg 
            180                 185                 190         


Thr Asn Arg Pro Thr Pro Leu Glu Glu Ala Lys Trp Gly Phe Ala Val 
        195                 200                 205             


Ile Glu Asn Ser Leu Trp Glu Gly Leu Pro Ala Phe Ile Arg Lys Leu 
    210                 215                 220                 


Asn Asp Ala Ala Val Glu His Leu Asn Tyr Ala Leu Pro Val Asp Leu 
225                 230                 235                 240 


Thr Pro Val Arg Phe Ser Ser Trp Met Gly Gly Asp Arg Asp Gly Asn 
                245                 250                 255     


Pro Phe Val Thr Ala Lys Ile Thr Arg Glu Ala Leu Gln Leu Ala Arg 
            260                 265                 270         


Trp Lys Ala Ala Asp Leu Phe Leu Thr Asp Ile Gln Glu Leu Cys Asp 
        275                 280                 285             


Glu Leu Ser Met Thr Gln Cys Thr Ala Glu Phe Arg Glu Lys Tyr Gly 
    290                 295                 300                 


Asp His Leu Glu Pro Tyr Arg Val Val Val Lys Asp Leu Arg Ser Lys 
305                 310                 315                 320 


Leu Lys Asn Thr Leu Asp Tyr Tyr Asn Asp Ile Leu Ala Gly Arg Ile 
                325                 330                 335     


Pro Pro Phe Lys Gln Asp Glu Ile Ile Ser Glu Asp Gln Gln Leu Trp 
            340                 345                 350         


Gln Pro Leu Tyr Asp Cys Tyr Gln Ser Leu Thr Ala Cys Gly Met Arg 
        355                 360                 365             


Ile Ile Ala Asn Gly Leu Leu Leu Asp Thr Leu Arg Arg Val Arg Cys 
    370                 375                 380                 


Phe Gly Val Thr Leu Leu Arg Leu Asp Ile Arg Gln Glu Ser Thr Arg 
385                 390                 395                 400 


His Ser Asp Ala Ile Gly Glu Ile Thr Arg Tyr Ile Gly Leu Gly Asp 
                405                 410                 415     


Tyr Ser Gln Trp Thr Glu Asp Asp Lys Gln Ala Phe Leu Ile Arg Glu 
            420                 425                 430         


Leu Ser Ser Arg Arg Pro Leu Ile Pro His Asn Trp Thr Pro Ser Glu 
        435                 440                 445             


His Thr Arg Glu Ile Leu Asp Thr Cys Lys Val Ile Ala Lys Gln Pro 
    450                 455                 460                 


Glu Gly Val Ile Ser Cys Tyr Ile Ile Ser Met Ala Arg Thr Ala Ser 
465                 470                 475                 480 


Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Ser Tyr 
                485                 490                 495     


His Leu Pro Val Val Pro Leu Phe Glu Thr Leu Asp Asp Leu Asp Ala 
            500                 505                 510         


Ser Lys Glu Val Met Thr Gln Leu Phe Asn Val Gly Trp Tyr Arg Gly 
        515                 520                 525             


Val Ile Lys Asn Arg Gln Met Ile Met Ile Gly Tyr Ser Asp Ser Ala 
    530                 535                 540                 


Lys Asp Ala Gly Met Met Ala Ala Ser Trp Ala Gln Tyr Arg Ala Gln 
545                 550                 555                 560 


Asp Ala Leu Val Lys Leu Cys Glu Gln Thr Gly Ile Glu Leu Thr Leu 
                565                 570                 575     


Phe His Gly Arg Gly Gly Thr Val Gly Arg Gly Gly Ala Pro Ala His 
            580                 585                 590         


Ala Ala Leu Leu Ser Gln Pro Pro Arg Ser Leu Lys Asn Gly Leu Arg 
        595                 600                 605             


Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Leu Gly Leu Pro Ala 
    610                 615                 620                 


Ile Ala Ala Glu Ser Leu Asp Leu Tyr Ala Ser Ala Ile Leu Glu Ala 
625                 630                 635                 640 


Asn Leu Leu Pro Pro Pro Glu Pro Lys Ala Ser Trp Cys Arg Val Met 
                645                 650                 655     


Asp Glu Leu Ala Val Ala Ser Cys Glu Ile Tyr Arg Asn Val Val Arg 
            660                 665                 670         


Gly Asp Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu Gln 
        675                 680                 685             


Glu Leu Ala Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Asn Pro 
    690                 695                 700                 


Asn Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala Trp 
705                 710                 715                 720 


Met Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Ala Ser 
                725                 730                 735     


Ile Arg Gln Ala Met Glu Ser Gly Lys Ala Ala Val Ile Glu Glu Met 
            740                 745                 750         


Cys Asn His Trp Pro Phe Phe Asn Thr Arg Ile Gly Met Leu Glu Met 
        755                 760                 765             


Val Phe Ser Lys Thr Asp Ser Trp Leu Ser Glu Tyr Tyr Asp Gln Arg 
    770                 775                 780                 


Leu Val Lys Lys Glu Leu Trp Tyr Leu Gly Glu Ser Leu Arg Lys Gln 
785                 790                 795                 800 


Leu Ser Glu Asp Ile Ala Thr Val Leu Arg Leu Ser Gly Lys Gly Asp 
                805                 810                 815     


Gln Leu Met Ser Asp Leu Pro Trp Val Ala Glu Ser Ile Ala Leu Arg 
            820                 825                 830         


Asn Val Tyr Thr Asp Pro Leu Asn Leu Leu Gln Val Glu Leu Leu Arg 
        835                 840                 845             


Arg Leu Arg Ala Asp Pro Glu His Pro Asn Pro Asp Ile Glu Gln Ala 
    850                 855                 860                 


Leu Met Ile Thr Ile Thr Gly Ile Ala Ala Gly Met Arg Asn Thr Gly 
865                 870                 875                 880 


<210>  116
<211>  4171
<212>  DNA
<213>  Artificial

<220>
<223>  I. orientalis FUM gene integration fragment

<400>  116
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttcctcg acatttgctg caacggcaac atcaatgtcc acgtttacac     1200

acctacattt atatctatat ttatatttat atttatttat ttatgctact tagcttctat     1260

agttagttaa tgcactcacg atattcaaaa ttgacaccct tcaactactc cctactattg     1320

tctactactg tctactactc ctctttacta tagctgctcc caataggctc caccaatagg     1380

ctctgtcaat acattttgcg ccgccacctt tcaggttgtg tcactcctga aggaccatat     1440

tgggtaatcg tgcaatttct ggaagagagt ccgcgagaag tgaggccccc actgtaaatc     1500

ctcgaggggg catggagtat ggggcatgga ggatggagga tggggggggg gggggggaaa     1560

ataggtagcg aaaggacccg ctatcacccc acccggagaa ctcgttgccg ggaagtcata     1620

tttcgacact ccggggagtc tataaaaggc gggttttgtc ttttgccagt tgatgttgct     1680

gagaggactt gtttgccgtt tcttccgatt taacagtata gaatcaacca ctgttaatta     1740

tacacgttat actaacacaa caaaaacaaa aacaacgaca acaacaacaa catctagata     1800

aaatgttagc tgctagatca ttaaaggcaa gaatgtcaac aagagctttc tcaactacct     1860

caattgcaaa aagaatcgaa aaagatgcat ttggtgacat tgaagtccca aatgagaaat     1920

attggggtgc tcaaactcaa agatctttac aaaatttcaa aattggtggt aagagagaag     1980

ttatgccaga accaatcatc aaatcttttg gtattttaaa gaaggctact gctaagatca     2040

atgctgagtc tggtgcttta gacccaaagt tatctgaagc catccaacaa gctgcaaccg     2100

aagtttatga aggtaaacta atggaccatt tcccattagt tgtctttcaa accggttctg     2160

gtactcaatc taacatgaat gccaatgaag tcatctctaa tagagcaatt gaaatcttgg     2220

gtggtgaatt aggctctaaa actccagtcc atcctaatga tcatgttaat atgtcccaat     2280

cttctaatga tactttccct actgtcatgc atattgcagc agttacagaa gtttcatccc     2340

atttattacc agaattaact gcactaagag atgcattgca aaagaaatcc gatgaattta     2400

agaatattat caaaatcggt agaacccatt tacaagatgc aactccttta actttaggtc     2460

aagaattttc tggttatgtt caacaatgta ctaatggtat caaaagaatc gaaattgctc     2520

ttgaacattt gagatactta gctcaaggtg gtactgccgt tggtactggt cttaacacca     2580

agaaaggttt tgctgaaaag gttgcaaatg aagtcactaa attgactggt ttacaattct     2640

ataccgctcc aaataaattc gaagcccttg cagctcacga tgctgttgtt gaaatgtctg     2700

gtgctttgaa taccgttgca gtctcattat tcaaaatcgc tcaagatatc agatatttgg     2760

gttccggccc aagatgtggt tatggtgaat tggctttacc agaaaatgaa ccaggttctt     2820

ccatcatgcc gggtaaagtt aacccaactc aaaacgaagc tttgactatg ctttgtaccc     2880

aagtctttgg taaccactct tgtattacct ttgcaggtgc ttcaggtcaa ttcgaattga     2940

atgtctttaa gccagttatg atctccaact tgttatcttc tattaggtta ttaggtgatg     3000

gttgtaattc ttttagaatc cactgtgttg aaggtatcat tgcaaatacc gacaagattg     3060

ataaattact acatgaatct ctcatgttag ttactgcttt gaacccacac attggttacg     3120

ataaggcttc caagattgca aagaatgcac acaagaaggg cttgacattg aaacaatctg     3180

cattggaatt aggttacttg accgaagaac aattcaatga atgggttaga ccagaaaaca     3240

tgattggtcc aaaggattaa gttaattaac atctgaatgt aaaatgaaca ttaaaatgaa     3300

ttactaaact ttacgtctac tttacaatct ataaactttg tttaatcata taacgaaata     3360

cactaataca caatcctgta cgtatgtaat acttttatcc atcaaggatt gagaaaaaaa     3420

agtaatgatt ccctgggcca ttaaaactta gacccccaag cttggatagg tcactctcta     3480

ttttcgtttc tcccttccct gatagaaggg tgatatgtaa ttaagaataa tatataattt     3540

tataataaaa gcggccgcac caggggttta gtgaagtcac caattaagat tgttggtttg     3600

agtgagttgc caaagatcta tgaattgatg gagcaaggta agattttagg cagatatgtt     3660

gttgacactt cgaaatgatg ggctgacttg ggtgtactgg tgtgacgttt ttatgtgtat     3720

attgatatgc atgggggatg tatagtgatg aggagtagag tatataacga aatgaaatga     3780

aataatatga tatgataaga taagatgaga tcaatacgat aatataagat gcgacatgag     3840

gagttcaatg tagcatacta cacgatgctg cagtacaact ctgatacgct agactatact     3900

atacaaaact gtagtacact atacgttagt gtagtatcca gaaacaacac tgctttatag     3960

tacaatacaa ctctataata ctatagtata ctatgccaaa ccacgtaata ccataatatg     4020

ctccacgaca tggtacaatg tgctatactt catactatta taccatatat actccgatat     4080

attattgata tactatttta tactataata ccataccaca caacactaca ttacaacgag     4140

caaccttacc ataaatgtca gttatggccg c                                    4171


<210>  117
<211>  466
<212>  PRT
<213>  Escherichia coli

<400>  117

Met Pro His Ser Tyr Asp Tyr Asp Ala Ile Val Ile Gly Ser Gly Pro 
1               5                   10                  15      


Gly Gly Glu Gly Ala Ala Met Gly Leu Val Lys Gln Gly Ala Arg Val 
            20                  25                  30          


Ala Val Ile Glu Arg Tyr Gln Asn Val Gly Gly Gly Cys Thr His Trp 
        35                  40                  45              


Gly Thr Ile Pro Ser Lys Ala Leu Arg His Ala Val Ser Arg Ile Ile 
    50                  55                  60                  


Glu Phe Asn Gln Asn Pro Leu Tyr Ser Asp His Ser Arg Leu Leu Arg 
65                  70                  75                  80  


Ser Ser Phe Ala Asp Ile Leu Asn His Ala Asp Asn Val Ile Asn Gln 
                85                  90                  95      


Gln Thr Arg Met Arg Gln Gly Phe Tyr Glu Arg Asn His Cys Glu Ile 
            100                 105                 110         


Leu Gln Gly Asn Ala Arg Phe Val Asp Glu His Thr Leu Ala Leu Asp 
        115                 120                 125             


Cys Pro Asp Gly Ser Val Glu Thr Leu Thr Ala Glu Lys Phe Val Ile 
    130                 135                 140                 


Ala Cys Gly Ser Arg Pro Tyr His Pro Thr Asp Val Asp Phe Thr His 
145                 150                 155                 160 


Pro Arg Ile Tyr Asp Ser Asp Ser Ile Leu Ser Met His His Glu Pro 
                165                 170                 175     


Arg His Val Leu Ile Tyr Gly Ala Gly Val Ile Gly Cys Glu Tyr Ala 
            180                 185                 190         


Ser Ile Phe Arg Gly Met Asp Val Lys Val Asp Leu Ile Asn Thr Arg 
        195                 200                 205             


Asp Arg Leu Leu Ala Phe Leu Asp Gln Glu Met Ser Asp Ser Leu Ser 
    210                 215                 220                 


Tyr His Phe Trp Asn Ser Gly Val Val Ile Arg His Asn Glu Glu Tyr 
225                 230                 235                 240 


Glu Lys Ile Glu Gly Cys Asp Asp Gly Val Ile Met His Leu Lys Ser 
                245                 250                 255     


Gly Lys Lys Leu Lys Ala Asp Cys Leu Leu Tyr Ala Asn Gly Arg Thr 
            260                 265                 270         


Gly Asn Thr Asp Ser Leu Ala Leu Gln Asn Ile Gly Leu Glu Thr Asp 
        275                 280                 285             


Ser Arg Gly Gln Leu Lys Val Asn Ser Met Tyr Gln Thr Ala Gln Pro 
    290                 295                 300                 


His Val Tyr Ala Val Gly Asp Val Ile Gly Tyr Pro Ser Leu Ala Ser 
305                 310                 315                 320 


Ala Ala Tyr Asp Gln Gly Arg Ile Ala Ala Gln Ala Leu Val Lys Gly 
                325                 330                 335     


Glu Ala Thr Ala His Leu Ile Glu Asp Ile Pro Thr Gly Ile Tyr Thr 
            340                 345                 350         


Ile Pro Glu Ile Ser Ser Val Gly Lys Thr Glu Gln Gln Leu Thr Ala 
        355                 360                 365             


Met Lys Val Pro Tyr Glu Val Gly Arg Ala Gln Phe Lys His Leu Ala 
    370                 375                 380                 


Arg Ala Gln Ile Val Gly Met Asn Val Gly Thr Leu Lys Ile Leu Phe 
385                 390                 395                 400 


His Arg Glu Thr Lys Glu Ile Leu Gly Ile His Cys Phe Gly Glu Arg 
                405                 410                 415     


Ala Ala Glu Ile Ile His Ile Gly Gln Ala Ile Met Glu Gln Lys Gly 
            420                 425                 430         


Gly Gly Asn Thr Ile Glu Tyr Phe Val Asn Thr Thr Phe Asn Tyr Pro 
        435                 440                 445             


Thr Met Ala Glu Ala Tyr Arg Val Ala Ala Leu Asn Gly Leu Asn Arg 
    450                 455                 460                 


Leu Phe 
465     


<210>  118
<211>  466
<212>  PRT
<213>  Artificial

<220>
<223>  Codon optimized E. coli Stha enzyme

<400>  118

Met Pro His Ser Tyr Asp Tyr Asp Ala Ile Val Ile Gly Ser Gly Pro 
1               5                   10                  15      


Gly Gly Glu Gly Ala Ala Met Gly Leu Val Lys Gln Gly Ala Arg Val 
            20                  25                  30          


Ala Val Ile Glu Arg Tyr Gln Asn Val Gly Gly Gly Cys Thr His Trp 
        35                  40                  45              


Gly Thr Ile Pro Ser Lys Ala Leu Arg His Ala Val Ser Arg Ile Ile 
    50                  55                  60                  


Glu Phe Asn Gln Asn Pro Leu Tyr Ser Asp His Ser Arg Leu Leu Arg 
65                  70                  75                  80  


Ser Ser Phe Ala Asp Ile Leu Asn His Ala Asp Asn Val Ile Asn Gln 
                85                  90                  95      


Gln Thr Arg Met Arg Gln Gly Phe Tyr Glu Arg Asn His Cys Glu Ile 
            100                 105                 110         


Leu Gln Gly Asn Ala Arg Phe Val Asp Glu His Thr Leu Ala Leu Asp 
        115                 120                 125             


Cys Pro Asp Gly Ser Val Glu Thr Leu Thr Ala Glu Lys Phe Val Ile 
    130                 135                 140                 


Ala Cys Gly Ser Arg Pro Tyr His Pro Thr Asp Val Asp Phe Thr His 
145                 150                 155                 160 


Pro Arg Ile Tyr Asp Ser Asp Ser Ile Leu Ser Met His His Glu Pro 
                165                 170                 175     


Arg His Val Leu Ile Tyr Gly Ala Gly Val Ile Gly Cys Glu Tyr Ala 
            180                 185                 190         


Ser Ile Phe Arg Gly Met Asp Val Lys Val Asp Leu Ile Asn Thr Arg 
        195                 200                 205             


Asp Arg Leu Leu Ala Phe Leu Asp Gln Glu Met Ser Asp Ser Leu Ser 
    210                 215                 220                 


Tyr His Phe Trp Asn Ser Gly Val Val Ile Arg His Asn Glu Glu Tyr 
225                 230                 235                 240 


Glu Lys Ile Glu Gly Cys Asp Asp Gly Val Ile Met His Leu Lys Ser 
                245                 250                 255     


Gly Lys Lys Leu Lys Ala Asp Cys Leu Leu Tyr Ala Asn Gly Arg Thr 
            260                 265                 270         


Gly Asn Thr Asp Ser Leu Ala Leu Gln Asn Ile Gly Leu Glu Thr Asp 
        275                 280                 285             


Ser Arg Gly Gln Leu Lys Val Asn Ser Met Tyr Gln Thr Ala Gln Pro 
    290                 295                 300                 


His Val Tyr Ala Val Gly Asp Val Ile Gly Tyr Pro Ser Leu Ala Ser 
305                 310                 315                 320 


Ala Ala Tyr Asp Gln Gly Arg Ile Ala Ala Gln Ala Leu Val Lys Gly 
                325                 330                 335     


Glu Ala Thr Ala His Leu Ile Glu Asp Ile Pro Thr Gly Ile Tyr Thr 
            340                 345                 350         


Ile Pro Glu Ile Ser Ser Val Gly Lys Thr Glu Gln Gln Leu Thr Ala 
        355                 360                 365             


Met Lys Val Pro Tyr Glu Val Gly Arg Ala Gln Phe Lys His Leu Ala 
    370                 375                 380                 


Arg Ala Gln Ile Val Gly Met Asn Val Gly Thr Leu Lys Ile Leu Phe 
385                 390                 395                 400 


His Arg Glu Thr Lys Glu Ile Leu Gly Ile His Cys Phe Gly Glu Arg 
                405                 410                 415     


Ala Ala Glu Ile Ile His Ile Gly Gln Ala Ile Met Glu Gln Lys Gly 
            420                 425                 430         


Gly Gly Asn Thr Ile Glu Tyr Phe Val Asn Thr Thr Phe Asn Tyr Pro 
        435                 440                 445             


Thr Met Ala Glu Ala Tyr Arg Val Ala Ala Leu Asn Gly Leu Asn Arg 
    450                 455                 460                 


Leu Phe 
465     


<210>  119
<211>  464
<212>  PRT
<213>  Azotobacter vinelandii

<400>  119

Met Ala Val Tyr Asn Tyr Asp Val Val Val Ile Gly Thr Gly Pro Ala 
1               5                   10                  15      


Gly Glu Gly Ala Ala Met Asn Ala Val Lys Ala Gly Arg Lys Val Ala 
            20                  25                  30          


Val Val Asp Asp Arg Pro Gln Val Gly Gly Asn Cys Thr His Leu Gly 
        35                  40                  45              


Thr Ile Pro Ser Lys Ala Leu Arg His Ser Val Arg Gln Ile Met Gln 
    50                  55                  60                  


Tyr Asn Asn Asn Pro Leu Phe Arg Gln Ile Gly Glu Pro Arg Trp Phe 
65                  70                  75                  80  


Ser Phe Ala Asp Val Leu Lys Ser Ala Glu Gln Val Ile Ala Lys Gln 
                85                  90                  95      


Val Ser Ser Arg Thr Gly Tyr Tyr Ala Arg Asn Arg Ile Asp Thr Phe 
            100                 105                 110         


Phe Gly Thr Ala Ser Phe Cys Asp Glu His Thr Ile Glu Val Val His 
        115                 120                 125             


Leu Asn Gly Met Val Glu Thr Leu Val Ala Lys Gln Phe Val Ile Ala 
    130                 135                 140                 


Thr Gly Ser Arg Pro Tyr Arg Pro Ala Asp Val Asp Phe Thr His Pro 
145                 150                 155                 160 


Arg Ile Tyr Asp Ser Asp Thr Ile Leu Ser Leu Gly His Thr Pro Arg 
                165                 170                 175     


Arg Leu Ile Ile Tyr Gly Ala Gly Val Ile Gly Cys Glu Tyr Ala Ser 
            180                 185                 190         


Ile Phe Ser Gly Leu Gly Val Leu Val Asp Leu Ile Asp Asn Arg Asp 
        195                 200                 205             


Gln Leu Leu Ser Phe Leu Asp Asp Glu Ile Ser Asp Ser Leu Ser Tyr 
    210                 215                 220                 


His Leu Arg Asn Asn Asn Val Leu Ile Arg His Asn Glu Glu Tyr Glu 
225                 230                 235                 240 


Arg Val Glu Gly Leu Asp Asn Gly Val Ile Leu His Leu Lys Ser Gly 
                245                 250                 255     


Lys Lys Ile Lys Ala Asp Ala Phe Leu Trp Ser Asn Gly Arg Thr Gly 
            260                 265                 270         


Asn Thr Asp Lys Leu Gly Leu Glu Asn Ile Gly Leu Lys Ala Asn Gly 
        275                 280                 285             


Arg Gly Gln Ile Gln Val Asp Glu His Tyr Arg Thr Glu Val Ser Asn 
    290                 295                 300                 


Ile Tyr Ala Ala Gly Asp Val Ile Gly Trp Pro Ser Leu Ala Ser Ala 
305                 310                 315                 320 


Ala Tyr Asp Gln Gly Arg Ser Ala Ala Gly Ser Ile Thr Glu Asn Asp 
                325                 330                 335     


Ser Trp Arg Phe Val Asp Asp Val Pro Thr Gly Ile Tyr Thr Ile Pro 
            340                 345                 350         


Glu Ile Ser Ser Val Gly Lys Thr Glu Arg Glu Leu Thr Gln Ala Lys 
        355                 360                 365             


Val Pro Tyr Glu Val Gly Lys Ala Phe Phe Lys Gly Met Ala Arg Ala 
    370                 375                 380                 


Gln Ile Ala Val Glu Lys Ala Gly Met Leu Lys Ile Leu Phe His Arg 
385                 390                 395                 400 


Glu Thr Leu Glu Ile Leu Gly Val His Cys Phe Gly Tyr Gln Ala Ser 
                405                 410                 415     


Glu Ile Val His Ile Gly Gln Ala Ile Met Asn Gln Lys Gly Glu Ala 
            420                 425                 430         


Asn Thr Leu Lys Tyr Phe Ile Asn Thr Thr Phe Asn Tyr Pro Thr Met 
        435                 440                 445             


Ala Glu Ala Tyr Arg Val Ala Ala Tyr Asp Gly Leu Asn Arg Leu Phe 
    450                 455                 460                 


<210>  120
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  120
cacagaggtg cagtaacgag                                                   20


<210>  121
<211>  506
<212>  PRT
<213>  Issatchenkia orientalis

<400>  121

Met Gly Val Gln Phe Ile Glu Asn Thr Ile Ile Val Val Phe Gly Ala 
1               5                   10                  15      


Ser Gly Asp Leu Ala Lys Lys Lys Thr Phe Pro Ala Leu Phe Gly Leu 
            20                  25                  30          


Phe Arg Glu Gly Gln Leu Ser Glu Thr Thr Lys Ile Ile Gly Phe Ala 
        35                  40                  45              


Arg Ser Lys Leu Ser Asn Asp Asp Leu Arg Asn Arg Ile Lys Pro Tyr 
    50                  55                  60                  


Leu Lys Leu Asn Lys Arg Thr Asp Ala Glu Arg Gln Ser Leu Glu Lys 
65                  70                  75                  80  


Phe Leu Gln Ile Leu Glu Tyr His Gln Ser Asn Tyr Asp Asp Ser Glu 
                85                  90                  95      


Gly Phe Glu Lys Leu Glu Lys Leu Ile Asn Lys Tyr Asp Asp Glu Ala 
            100                 105                 110         


Asn Val Lys Glu Ser His Arg Leu Tyr Tyr Leu Ala Leu Pro Pro Ser 
        115                 120                 125             


Val Phe Thr Thr Val Ala Thr Met Leu Lys Lys His Cys His Pro Gly 
    130                 135                 140                 


Asp Ser Gly Ile Ala Arg Leu Ile Val Glu Lys Pro Phe Gly His Asp 
145                 150                 155                 160 


Leu Ser Ser Ser Arg Glu Leu Gln Lys Ser Leu Ala Pro Leu Trp Asn 
                165                 170                 175     


Glu Asp Glu Leu Phe Arg Ile Asp His Tyr Leu Gly Lys Glu Met Val 
            180                 185                 190         


Lys Asn Leu Ile Pro Leu Arg Phe Ser Asn Thr Phe Leu Ser Ser Ser 
        195                 200                 205             


Trp Asn Asn Gln Phe Ile Asp Thr Ile Gln Ile Thr Phe Lys Glu Asn 
    210                 215                 220                 


Phe Gly Thr Glu Gly Arg Gly Gly Tyr Phe Asp Ser Ile Gly Ile Ile 
225                 230                 235                 240 


Arg Asp Val Ile Gln Asn His Leu Leu Gln Val Leu Thr Ile Val Leu 
                245                 250                 255     


Met Glu Lys Pro Ala Asp Phe Asn Gly Glu Ser Ile Arg Asp Glu Lys 
            260                 265                 270         


Val Lys Val Leu Lys Ala Ile Glu Gln Ile Asp Phe Asn Asn Val Leu 
        275                 280                 285             


Val Gly Gln Tyr Asp Lys Ser Glu Asp Gly Ser Lys Pro Gly Tyr Leu 
    290                 295                 300                 


Asp Asp Asp Thr Val Asn Pro Asp Ser Lys Ala Val Thr Tyr Ala Ala 
305                 310                 315                 320 


Leu Val Leu Asn Val Ala Asn Glu Arg Trp Asn Asn Val Pro Ile Ile 
                325                 330                 335     


Leu Lys Ala Gly Lys Ala Leu Asn Gln Ser Lys Val Glu Ile Arg Ile 
            340                 345                 350         


Gln Phe Lys Pro Val Glu Asn Gly Ile Phe Lys Asn Ser Ala Arg Asn 
        355                 360                 365             


Glu Leu Val Ile Arg Ile Gln Pro Asn Glu Ala Met Tyr Leu Lys Met 
    370                 375                 380                 


Asn Ile Lys Val Pro Gly Val Ser Asn Gln Val Ser Ile Ser Glu Met 
385                 390                 395                 400 


Asp Leu Thr Tyr Lys Asn Arg Tyr Ser Ser Glu Phe Tyr Ile Pro Glu 
                405                 410                 415     


Ala Tyr Glu Ser Leu Ile Lys Asp Ala Leu Met Asp Asp His Ser Asn 
            420                 425                 430         


Phe Val Arg Asp Asp Glu Leu Asp Ile Ser Trp Ala Leu Phe Thr Pro 
        435                 440                 445             


Leu Leu Glu His Ile Glu Gly Pro Asp Gly Pro Thr Pro Thr Lys Tyr 
    450                 455                 460                 


Pro Tyr Gly Ser Arg Gly Pro Lys Glu Ile Asp Glu Phe Leu Arg Asn 
465                 470                 475                 480 


His Gly Tyr Val Lys Glu Pro Arg Glu Asn Tyr Gln Trp Pro Leu Thr 
                485                 490                 495     


Thr Pro Lys Glu Leu Asn Ser Ser Lys Phe 
            500                 505     


<210>  122
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  122
gctggagaat agatcttcaa cgccccg                                           27


<210>  123
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  123
catcactgtt aaaggaatgg gtaaatc                                           27


<210>  124
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  124
gtaagctggc aaacctgcag gttagccggt attacgcata c                           41


<210>  125
<211>  4321
<212>  DNA
<213>  Artificial

<220>
<223>  I. orientalis FUM integration fragment

<400>  125
ttgaaggagc ttgccaagaa acataatttt atgatttttg aagatagaaa atttgctgat       60

attggtaaca ctgttaaaaa tcaatataaa tctggtgtct tccgtattgc cgaatgggct      120

gacatcacta atgcacatgg tgtaacgggt gcaggtattg tttctggctt gaaggaggca      180

gcccaagaaa caaccagtga acctagaggt ttgctaatgc ttgctgagtt atcatcaaag      240

ggttctttag catatggtga atatacagaa aaaacagtag aaattgctaa atctgataaa      300

gagtttgtca ttggttttat tgcgcaacac gatatgggcg gtagagaaga aggttttgac      360

tggatcatta tgactccagg ggttggttta gatgacaaag gtgatgcact tggtcaacaa      420

tatagaactg ttgatgaagt tgtaaagact ggaacggata tcataattgt tggtagaggt      480

ttgtacggtc aaggaagaga tcctatagag caagctaaaa gataccaaca agctggttgg      540

aatgcttatt taaacagatt taaatgattc ttacacaaag atttgataca tgtacactag      600

tttaaataag catgaaaaga attacacaag caaaaaaaaa aaaataaatg aggtacttta      660

cgttcaccta caaccaaaaa aactagatag agtaaaatct taagatttag aaaaagttgt      720

ttaacaaagg ctttagtatg tgaattttta atgtagcaaa gcgataacta ataaacataa      780

acaaaagtat ggttttcttt atcagtcaaa tcattatcga ttgattgttc cgcgtatctg      840

cagatagcct catgaaatca gccatttgct tttgttcaac gatcttttga aattgttgtt      900

gttcttggta gttaagttga tccatcttgg cttatgttgt gtgtatgttg tagttattct      960

tagtatattc ctgtcctgag tttagtgaaa cataatatcg ccttgaaatg aaaatgctga     1020

aattcgtcga catacaattt ttcaaacttt ttttttttct tggtgcacgg acatgttttt     1080

aaaggaagta ctctatacca gttattcttc acaaatttaa ttgctggaga atagatcttc     1140

aacgcgtttc ctcgacattt gctgcaacgg caacatcaat gtccacgttt acacacctac     1200

atttatatct atatttatat ttatatttat ttatttatgc tacttagctt ctatagttag     1260

ttaatgcact cacgatattc aaaattgaca cccttcaact actccctact attgtctact     1320

actgtctact actcctcttt actatagctg ctcccaatag gctccaccaa taggctctgt     1380

caatacattt tgcgccgcca cctttcaggt tgtgtcactc ctgaaggacc atattgggta     1440

atcgtgcaat ttctggaaga gagtccgcga gaagtgaggc ccccactgta aatcctcgag     1500

ggggcatgga gtatggggca tggaggatgg aggatggggg gggggggggg gaaaataggt     1560

agcgaaagga cccgctatca ccccacccgg agaactcgtt gccgggaagt catatttcga     1620

cactccgggg agtctataaa aggcgggttt tgtcttttgc cagttgatgt tgctgagagg     1680

acttgtttgc cgtttcttcc gatttaacag tatagaatca accactgtta attatacacg     1740

ttatactaac acaacaaaaa caaaaacaac gacaacaaca acaacatcta gataaaatgt     1800

tagctgctag atcattaaag gcaagaatgt caacaagagc tttctcaact acctcaattg     1860

caaaaagaat cgaaaaagat gcatttggtg acattgaagt cccaaatgag aaatattggg     1920

gtgctcaaac tcaaagatct ttacaaaatt tcaaaattgg tggtaagaga gaagttatgc     1980

cagaaccaat catcaaatct tttggtattt taaagaaggc tactgctaag atcaatgctg     2040

agtctggtgc tttagaccca aagttatctg aagccatcca acaagctgca accgaagttt     2100

atgaaggtaa actaatggac catttcccat tagttgtctt tcaaaccggt tctggtactc     2160

aatctaacat gaatgccaat gaagtcatct ctaatagagc aattgaaatc ttgggtggtg     2220

aattaggctc taaaactcca gtccatccta atgatcatgt taatatgtcc caatcttcta     2280

atgatacttt ccctactgtc atgcatattg cagcagttac agaagtttca tcccatttat     2340

taccagaatt aactgcacta agagatgcat tgcaaaagaa atccgatgaa tttaagaata     2400

ttatcaaaat cggtagaacc catttacaag atgcaactcc tttaacttta ggtcaagaat     2460

tttctggtta tgttcaacaa tgtactaatg gtatcaaaag aatcgaaatt gctcttgaac     2520

atttgagata cttagctcaa ggtggtactg ccgttggtac tggtcttaac accaagaaag     2580

gttttgctga aaaggttgca aatgaagtca ctaaattgac tggtttacaa ttctataccg     2640

ctccaaataa attcgaagcc cttgcagctc acgatgctgt tgttgaaatg tctggtgctt     2700

tgaataccgt tgcagtctca ttattcaaaa tcgctcaaga tatcagatat ttgggttccg     2760

gcccaagatg tggttatggt gaattggctt taccagaaaa tgaaccaggt tcttccatca     2820

tgccgggtaa agttaaccca actcaaaacg aagctttgac tatgctttgt acccaagtct     2880

ttggtaacca ctcttgtatt acctttgcag gtgcttcagg tcaattcgaa ttgaatgtct     2940

ttaagccagt tatgatctcc aacttgttat cttctattag gttattaggt gatggttgta     3000

attcttttag aatccactgt gttgaaggta tcattgcaaa taccgacaag attgataaat     3060

tactacatga atctctcatg ttagttactg ctttgaaccc acacattggt tacgataagg     3120

cttccaagat tgcaaagaat gcacacaaga agggcttgac attgaaacaa tctgcattgg     3180

aattaggtta cttgaccgaa gaacaattca atgaatgggt tagaccagaa aacatgattg     3240

gtccaaagga ttaagttaat taacatctga atgtaaaatg aacattaaaa tgaattacta     3300

aactttacgt ctactttaca atctataaac tttgtttaat catataacga aatacactaa     3360

tacacaatcc tgtacgtatg taatactttt atccatcaag gattgagaaa aaaaagtaat     3420

gattccctgg gccattaaaa cttagacccc caagcttgga taggtcactc tctattttcg     3480

tttctccctt ccctgataga agggtgatat gtaattaaga ataatatata attttataat     3540

aaaagcggcc gcctcccttc tctaaatgga ctgcttggat aacttggacc cccttcccat     3600

tttatagtca ttctcttccc cctcattttc ccactattcc caacaatgac catctctcca     3660

ccttgtttcc ccattcttcc tgctctacct ggtgggggtg tttcacccca ttaacggtcg     3720

gattccgctg tggagatggc tctggccttt ttcccattcc ttccccccct caatcttctc     3780

catgcgggga aaaaaaaatt ttatccataa acaaccaaac cggcggctca acggggggtt     3840

tatactgaca gaaatggggt caatacaccc actgactgta cccgctctaa tcttaagctt     3900

tccccccccc ctcctgtatt aacggcgcgg agtgcccgca gcgcccaatg gagaaggcgc     3960

gcagtggggg atgcccaggg aggggacagg tacacgcaca ggccatgcca acaccgcata     4020

gacgtgcgac ctcctctccc ccactgcaga gctgcccttt tcggacacac tccgtgcaag     4080

aggactcggc cggctcggct tttctgccga attcggcagc cctgatattg tcttacgtaa     4140

tacgaatgga gggggtgtct cattttccct gcgatttccc aattgggtag caatgtgcac     4200

acactgaaac agtgcagagt attggtgatt tgctaatgta tggtaatgta taatactttt     4260

ttagagtgta gtgcattgca gacagtatag tatccacttc tgggaatccc atcgaaacgg     4320

c                                                                     4321


<210>  126
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  126
gatcgagctc caccttattt atgggagtta tttc                                   34


<210>  127
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  127
ggataaaagt attacatacg tacaggattg tgtattagtg tatttcg                     47


<210>  128
<211>  328
<212>  PRT
<213>  Rhizopus delemar

<400>  128

Met Val Lys Val Thr Val Cys Gly Ala Ala Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Lys Gln Ser Ser His Ile Thr His Leu Ser Leu 
            20                  25                  30          


Tyr Asp Ile Val Asn Thr Pro Gly Val Ala Ala Asp Leu Ser His Ile 
        35                  40                  45              


Asp Thr Lys Ser Lys Val Thr Gly His Val Gly Ala Ala Gln Leu Glu 
    50                  55                  60                  


Glu Ala Ile Lys Asp Ser Asp Val Val Val Ile Pro Ala Gly Val Pro 
65                  70                  75                  80  


Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Lys Ile Asn Ala Gly 
                85                  90                  95      


Ile Val Arg Asp Leu Ala Thr Ala Ala Ala Lys Tyr Ala Pro Lys Ala 
            100                 105                 110         


Phe Met Cys Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile Val 
        115                 120                 125             


Thr Glu Val Phe Lys Gln His Asn Val Tyr Asp Pro Lys Arg Ile Phe 
    130                 135                 140                 


Gly Val Thr Thr Leu Asp Ile Val Arg Ala Ser Thr Phe Val Ser Glu 
145                 150                 155                 160 


Leu Ile Gly Gly Glu Pro Asn Ser Leu Arg Val Pro Val Ile Gly Gly 
                165                 170                 175     


His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Ile 
            180                 185                 190         


Glu Lys Leu Asn Gln Glu Gln Ile Glu Lys Val Thr His Arg Ile Gln 
        195                 200                 205             


Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala 
    210                 215                 220                 


Thr Leu Ser Met Ala Tyr Ala Gly Ala Arg Phe Ala Thr Asn Ile Ile 
225                 230                 235                 240 


Glu Ala Ala Phe Ala Gly Lys Lys Gly Ile Val Glu Cys Thr Tyr Val 
                245                 250                 255     


Gln Leu Asp Ala Asp Lys Ser Gly Ala Gln Ser Val Lys Asp Leu Val 
            260                 265                 270         


Gly Ser Glu Leu Glu Tyr Phe Ser Val Pro Val Glu Leu Gly Pro Ser 
        275                 280                 285             


Gly Val Glu Lys Ile Leu Pro Ile Gly Asn Val Asn Glu Tyr Glu Lys 
    290                 295                 300                 


Lys Leu Leu Asn Glu Ala Ser Pro Glu Leu Lys Thr Asn Ile Asp Lys 
305                 310                 315                 320 


Gly Cys Thr Phe Val Thr Glu Gly 
                325             


<210>  129
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  129
tggcccaggg aatcattac                                                    19


<210>  130
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  130
tcaccacctg tcagtgacga gccacttc                                          28


<210>  131
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  131
ggacccaatg cctcccaatc                                                   20


<210>  132
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  132
cgtttctccc ttccctgata g                                                 21


<210>  133
<211>  6890
<212>  DNA
<213>  Artificial

<220>
<223>  E. coli SthA MEL integration fragment


<220>
<221>  misc_feature
<222>  (3973)..(3973)
<223>  n is a, c, g, or t

<400>  133
cggatttggt tggttcatag ctcttcttta gcgttgattg tagcctctgt tgcagaaaag       60

accttgtttt caagaaactg gttggtctga gtgttctgac accaatggtt atatttctag      120

ttgcaaacat gttggaataa tggtagtaat gttgatgctg gcagtgacag tagtgctagt      180

tcttgttctt gttctcgttc tcgttctcgt tctctttttt gtgctgtagc tgttactgta      240

ttggctactc tatataatat gcttgcaaag gaaaggaaat ctatgcaaac cactctctcc      300

tgcacaaacg ctagttcctt tgtcagggtt gaatgtagcc actctacgaa tgcgattcct      360

cttcccctct ctttcgcgtt gagcatattc aaaattgtga gaggtggcaa ggaaaaccat      420

acgattttcg gggtgcacgg atgcacagtg gacgtaagat ctgctctatt taagatacta      480

aagaaagtgg cagcgggaag accatcatgg aggacaacgt acatggacga ttccctgcta      540

gaaacaatga catgcaaacc cgtgcagtag gtagaagcag atttgctgag acagccatgc      600

caagtggaat tgttgtttaa ctctagtaga tattattgtt atagaaaaga tttatatata      660

agatccatgg aggggggagg gaggtaagag aaatacgaaa aagaatgtgt aatgaatctt      720

aatgtagaca agtggaaatg cagctaaagg gggtcaaagg gaatgtgata atgcaaggtt      780

aggtttaaca agaatgggtt ggcggactcg tcaatggaga gtacaatgcc aaagttctcc      840

ctgaggttat tgcggccgcg gatccctcga gattggtagt tctttccccc tctcaagctg      900

gcgtgaaatg caaccttacg gcgtctacgt tactacaagg tccagaaagt gtaggtattg      960

ctactatttt tattttttat tggttctgga gaaatgcaga cagtcaatga acacaactgt     1020

ctcaatatgc atctatgcac atgcacacac acacacatca caggtacccc tacaaagaga     1080

ggtctcttga taatgtttca ttaccacgtg gcatcccccc cccccccccc aataaacaag     1140

tggccgagtt cccctgttgc agaggaggac aaaaaaaccg ctggtgttgg taccattatg     1200

cagcaactag cacaacaaac aaccgaccca gacatacaaa tcaacaacac ttcgccaaag     1260

acaccctttc cagggaggat ccactcccaa cgtctctcca taatgtctct gttggcccat     1320

gtctctgtcg ttgacaccgt aaccacacca accaacccgt ccattgtact gggatggtcg     1380

tccatagaca cctctccaac ggggaacacc tcattcgtaa accgccaagg ttaccgttcc     1440

tcctgactcg ccccgttgtt gatgctgcgc acctgtggtt gcccaacatg gttgtatatc     1500

gtgtaaccac accaacacat gtgcagcaca tgtgtttaaa agagtgtcat ggaggtggat     1560

catgatggaa gtggacttta ccacttggga actgtctcca ctcccgggaa gaaaagaccc     1620

ggcgtatcac gcggttgcct caatggggca atttggaagg agaaatatag ggaaaatcac     1680

gtcgctctcg gacggggaag agttccagac tatgaggggg gggggtggta tataaagaca     1740

ggagatgtcc acccccagag agaggaagaa gttggaactt tagaagagag agataacttt     1800

ccccagtgtc catcaataca caaccaaaca caaactctat atttacacat ataaccccct     1860

ctctagaatg ccacattcct atgactacga tgccattgtc attggttccg gtccaggtgg     1920

tgaaggtgct gcaatgggct tagttaagca gggtgctaga gttgctgtca tcgaaagata     1980

tcaaaatgtt ggtggtggtt gtactcactg gggtacaatt ccatctaagg cattgagaca     2040

tgcagtttcc agaattattg agtttaacca aaacccttta tactctgatc attcaagatt     2100

gttgagatca tcttttgctg atattttgaa ccatgctgac aacgtcatca accaacaaac     2160

tcgtatgcgt caaggcttct atgagagaaa tcattgtgag attttacaag gtaacgctag     2220

atttgtcgat gagcatactc ttgcattaga ctgtccagac ggttccgttg agactcttac     2280

cgctgaaaaa ttcgttattg cttgtggttc cagaccatac cacccaaccg atgtcgattt     2340

cactcaccct cgtatctacg attccgattc tattttgtct atgcatcatg aaccaagaca     2400

tgttttgatt tatggtgctg gtgttatcgg ttgtgaatat gcttctattt tcagaggtat     2460

ggatgttaag gttgacttga ttaatacaag agacagatta ttagctttcc ttgatcagga     2520

aatgtctgat tccctttcct accatttttg gaactccggt gtcgtcatca gacacaacga     2580

ggaatatgaa aagattgaag gttgtgatga cggcgttatt atgcacctta agtctggtaa     2640

aaagttaaaa gcagattgct tgttatatgc aaatggtaga accggtaaca cagactcctt     2700

ggctttacaa aacattggtt tagaaaccga ttcaagaggt caattaaagg tcaattcaat     2760

gtatcaaact gcacaaccac acgtttacgc agttggtgac gttattggtt acccttcatt     2820

ggcatctgcc gcttacgatc aaggtagaat cgccgctcaa gcacttgtta agggtgaagc     2880

aactgcacac ttaatcgaag atatccctac cggtatctac actatcccag aaatctcttc     2940

tgttggcaag actgaacaac aattaaccgc aatgaaggtt ccatacgaag tcggtcgtgc     3000

ccagttcaag catttggcta gagcacaaat tgttggtatg aatgttggta ctttgaaaat     3060

cttgtttcac agagaaacaa aggaaatctt gggcattcac tgtttcggcg aaagagctgc     3120

agagattatt cacatcggtc aagccattat ggaacaaaaa ggcggtggta ataccattga     3180

atatttcgtt aataccacct tcaactaccc aacaatggcc gaagcatata gagtcgctgc     3240

tttaaacggt ttaaacagat tgttttaatt aacatctgaa tgtaaaatga acattaaaat     3300

gaattactaa actttacgtc tactttacaa tctataaact ttgtttaatc atataacgaa     3360

atacactaat acacaatcct gtacgtatgt aatactttta tccatcaagg attgagaaaa     3420

aaaagtaatg attccctggg ccattaaaac ttagaccccc aagcttggat aggtcactct     3480

ctattttcgt ttctcccttc cctgatagaa gggtgatatg taattaagaa taatatataa     3540

ttttataata aaagaattcg cccttacctg cagggataac ttcgtataat gtatgctata     3600

cgaagttatg ctgcaacggc aacatcaatg tccacgttta cacacctaca tttatatcta     3660

tatttatatt tatatttatt tatttatgct acttagcttc tatagttagt taatgcactc     3720

acgatattca aaattgacac ccttcaacta ctccctacta ttgtctacta ctgtctacta     3780

ctcctcttta ctatagctgc tcccaatagg ctccaccaat aggctctgtc aatacatttt     3840

gcgccgccac ctttcaggtt gtgtcactcc tgaaggacca tattgggtaa tcgtgcaatt     3900

tctggaagag agtgccgcga gaagtgaggc ccccactgta aatcctcgag ggggcatgga     3960

gtatggggca tgnaggatgg aggatggggg ggggggggga aaataggtag cgaaaggacc     4020

cgctatcacc ccacccggag aactcgttgc cgggaagtca tatttcgaca ctccggggag     4080

tctataaaag gcgggttttg tcttttgcca gttgatgttg ctgagaggac ttgtttgccg     4140

tttcttccga tttaacagta tagaatcaac cactgttaat tatacacgtt atactaacac     4200

aacaaaaaca aaaacaacga caacaacaac aacaatgttt gctttctact ttctcaccgc     4260

atgcaccact ttgaagggtg ttttcggagt ttctccgagt tacaatggtc ttggtctcac     4320

cccacagatg ggttgggaca gctggaatac gtttgcctgc gatgtcagtg aacagctact     4380

tctagacact gctgatagaa tttctgactt ggggctaaag gatatgggtt acaagtatgt     4440

catcctagat gactgttggt ctagcggcag ggattccgac ggtttcctcg ttgcagacaa     4500

gcacaaattt cccaacggta tgggccatgt tgcagaccac ctgcataata acagctttct     4560

tttcggtatg tattcgtctg ctggtgagta cacctgtgct gggtaccctg ggtctctggg     4620

gcgtgaggaa gaagatgctc aattctttgc aaataaccgc gttgactact tgaagtatga     4680

taattgttac aataaaggtc aatttggtac accagacgtt tcttaccacc gttacaaggc     4740

catgtcagat gctttgaata aaactggtag gcctattttc tattctctat gtaactgggg     4800

tcaggatttg acattttact ggggctctgg tatcgccaat tcttggagaa tgagcggaga     4860

tattactgct gagttcaccc gtccagatag cagatgtccc tgtgacggtg acgaatatga     4920

ttgcaagtac gccggtttcc attgttctat tatgaatatt cttaacaagg cagctccaat     4980

ggggcaaaat gcaggtgttg gtggttggaa cgatctggac aatctagagg tcggagtcgg     5040

taatttgact gacgatgagg aaaaggccca tttctctatg tgggcaatgg taaagtcccc     5100

acttatcatt ggtgccgacg tgaatcactt aaaggcatct tcgtactcga tctacagtca     5160

agcctctgtc atcgcaatta atcaagatcc aaagggtatt ccagccacaa gagtctggag     5220

atattatgtt tcagacaccg atgaatatgg acaaggtgaa attcaaatgt ggagtggtcc     5280

gcttgacaat ggtgaccaag tggttgcttt attgaatgga ggaagcgtag caagaccaat     5340

gaacacgacc ttggaagaga ttttctttga cagcaatttg ggttcaaagg aactgacatc     5400

gacttgggat atttacgact tatgggccaa cagagttgac aactctacgg cgtctgctat     5460

ccttgaacag aataaggcag ccaccggtat tctctacaat gctacagagc agtcttataa     5520

agacggtttg tctaagaatg atacaagact gtttggccag aaaattggta gtctttctcc     5580

aaatgctata cttaacacaa ctgttccagc tcatggtatc gccttctata ggttgagacc     5640

ctcggcttaa gctcaatgtt gagcaaagca ggacgagaaa aaaaaaaata atgattgtta     5700

agaagttcat gaaaaaaaaa aggaaaaata ctcaaatact tataacagag tgattaaata     5760

ataaacggca gtatacccta tcaggtattg agatagtttt atttttgtag gtatataatc     5820

tgaagccttt gaactatttt ctcgtatata tcatggagta tacattgcat tagcaacatt     5880

gcatactagt tcataacttc gtataatgta tgctatacga agttattaat taacaagggc     5940

gaattccttg atttatatac acctttgcca accgcttgtt acttgataag gaaaagatag     6000

atttctaaag tgcaggaaaa gaaacgccac tacgtcatga aacaaaagaa atgaaacact     6060

ctgcaaaagg gaaaaccaat gacgccttca aaacgtactg actttccgcc tccttttctg     6120

cctttttttt ttctccctca atttgccaat tcccctttcc gctaatttta catcaccttt     6180

ttgtttgttt cccttttcgg ccaagttttc catttctttt ttcggctgag cccttctttg     6240

gcgtcgacgt aatttctcgg catgtggcca atgtatattg acagtagatg aagtagacgt     6300

tcttagtaac tgttagggtg agattgccac ccccccttcc ttcttttact atctgtaata     6360

ccatcaccat agcaatagtt taaccatgtt ggagctggaa atacaacgtc tatagaggga     6420

agtcatcata ttacgccatt ttacggacca gggacaccct gtagtgtgtt tcctctcttg     6480

tagaggtagg ttttcaaatg gactctggcg tcgatttcca gcaagtcatt cccgtggttc     6540

accatttcta ctttttgcgc tacctctctt gacacagaaa tgaatgatga cgtgtaaatt     6600

acccgtccga gacctggact ccggagaaac tgtattaatt acgcgccaaa caagacaggt     6660

gtcggataaa cgtgcatgta cagactgcga gccgaaaacg gaagggggga aagaaaacag     6720

tggagtccca ttgttgttcc ggaaatggaa aacgggaact ggcggaaaag aaacgaaaca     6780

aaacaaaaga aaaagaggaa aaaaaagaaa aaaaaaagaa aaagacactg cacgtgattg     6840

ctggtgtgtg ctgcgtaacc gcggcacttt atttcgtaaa tgaaggggcc                6890


<210>  134
<211>  1656
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  134
atgtttgctt tctactttct caccgcatgc accactttga agggtgtttt cggagtttct       60

ccgagttaca atggtcttgg tctcacccca cagatgggtt gggacagctg gaatacgttt      120

gcctgcgatg tcagtgaaca gctacttcta gacactgctg atagaatttc tgacttgggg      180

ctaaaggata tgggttacaa gtatgtcatc ctagatgact gttggtctag cggcagggat      240

tccgacggtt tcctcgttgc agacaagcac aaatttccca acggtatggg ccatgttgca      300

gaccacctgc ataataacag ctttcttttc ggtatgtatt cgtctgctgg tgagtacacc      360

tgtgctgggt accctgggtc tctggggcgt gaggaagaag atgctcaatt ctttgcaaat      420

aaccgcgttg actacttgaa gtatgataat tgttacaata aaggtcaatt tggtacacca      480

gacgtttctt accaccgtta caaggccatg tcagatgctt tgaataaaac tggtaggcct      540

attttctatt ctctatgtaa ctggggtcag gatttgacat tttactgggg ctctggtatc      600

gccaattctt ggagaatgag cggagatatt actgctgagt tcacccgtcc agatagcaga      660

tgtccctgtg acggtgacga atatgattgc aagtacgccg gtttccattg ttctattatg      720

aatattctta acaaggcagc tccaatgggg caaaatgcag gtgttggtgg ttggaacgat      780

ctggacaatc tagaggtcgg agtcggtaat ttgactgacg atgaggaaaa ggcccatttc      840

tctatgtggg caatggtaaa gtccccactt atcattggtg ccgacgtgaa tcacttaaag      900

gcatcttcgt actcgatcta cagtcaagcc tctgtcatcg caattaatca agatccaaag      960

ggtattccag ccacaagagt ctggagatat tatgtttcag acaccgatga atatggacaa     1020

ggtgaaattc aaatgtggag tggtccgctt gacaatggtg accaagtggt tgctttattg     1080

aatggaggaa gcgtagcaag accaatgaac acgaccttgg aagagatttt ctttgacagc     1140

aatttgggtt caaaggaact gacatcgact tgggatattt acgacttatg ggccaacaga     1200

gttgacaact ctacggcgtc tgctatcctt gaacagaata aggcagccac cggtattctc     1260

tacaatgcta cagagcagtc ttataaagac ggtttgtcta agaatgatac aagactgttt     1320

ggccagaaaa ttggtagtct ttctccaaat gctatactta acacaactgt tccagctcat     1380

ggtatcgcct tctataggtt gagaccctcg gcttaagctc aatgttgagc aaagcaggac     1440

gagaaaaaaa aaaataatga ttgttaagaa gttcatgaaa aaaaaaagga aaaatactca     1500

aatacttata acagagtgat taaataataa acggcagtat accctatcag gtattgagat     1560

agttttattt ttgtaggtat ataatctgaa gcctttgaac tattttctcg tatatatcat     1620

ggagtataca ttgcattagc aacattgcat actagt                               1656


<210>  135
<211>  6279
<212>  DNA
<213>  Artificial

<220>
<223>  E. coli SthA URA integration fragment

<400>  135
ggccccttca tttacgaaat aaagtgccgc ggttacgcag cacacaccag caatcacgtg       60

cagtgtcttt ttcttttttt tttctttttt ttcctctttt tcttttgttt tgtttcgttt      120

cttttccgcc agttcccgtt ttccatttcc ggaacaacaa tgggactcca ctgttttctt      180

tccccccttc cgttttcggc tcgcagtctg tacatgcacg tttatccgac acctgtcttg      240

tttggcgcgt aattaataca gtttctccgg agtccaggtc tcggacgggt aatttacacg      300

tcatcattca tttctgtgtc aagagaggta gcgcaaaaag tagaaatggt gaaccacggg      360

aatgacttgc tggaaatcga cgccagagtc catttgaaaa cctacctcta caagagagga      420

aacacactac agggtgtccc tggtccgtaa aatggcgtaa tatgatgact tccctctata      480

gacgttgtat ttccagctcc aacatggtta aactattgct atggtgatgg tattacagat      540

agtaaaagaa ggaagggggg gtggcaatct caccctaaca gttactaaga acgtctactt      600

catctactgt caatatacat tggccacatg ccgagaaatt acgtcgacgc caaagaaggg      660

ctcagccgaa aaaagaaatg gaaaacttgg ccgaaaaggg aaacaaacaa aaaggtgatg      720

taaaattagc ggaaagggga attggcaaat tgagggagaa aaaaaaaagg cagaaaagga      780

ggcggaaagt cagtacgttt tgaaggcgtc attggttttc ccttttgcag agtgtttcat      840

ttcttttgtt tcatgacgta gtggcgtttc ttttcctgca ctttagaaat ctatcttttc      900

cttatcaagt aacaagcggt tggcaaaggt gtatataaat caaggaattc ccactttgaa      960

ccctttgaat tttgatatcg tttattttaa atttatttgc ggccgcggat ccctcgagat     1020

tggtagttct ttccccctct caagctggcg tgaaatgcaa ccttacggcg tctacgttac     1080

tacaaggtcc agaaagtgta ggtattgcta ctatttttat tttttattgg ttctggagaa     1140

atgcagacag tcaatgaaca caactgtctc aatatgcatc tatgcacatg cacacacaca     1200

cacatcacag gtacccctac aaagagaggt ctcttgataa tgtttcatta ccacgtggca     1260

tccccccccc cccccccaat aaacaagtgg ccgagttccc ctgttgcaga ggaggacaaa     1320

aaaaccgctg gtgttggtac cattatgcag caactagcac aacaaacaac cgacccagac     1380

atacaaatca acaacacttc gccaaagaca ccctttccag ggaggatcca ctcccaacgt     1440

ctctccataa tgtctctgtt ggcccatgtc tctgtcgttg acaccgtaac cacaccaacc     1500

aacccgtcca ttgtactggg atggtcgtcc atagacacct ctccaacggg gaacacctca     1560

ttcgtaaacc gccaaggtta ccgttcctcc tgactcgccc cgttgttgat gctgcgcacc     1620

tgtggttgcc caacatggtt gtatatcgtg taaccacacc aacacatgtg cagcacatgt     1680

gtttaaaaga gtgtcatgga ggtggatcat gatggaagtg gactttacca cttgggaact     1740

gtctccactc ccgggaagaa aagacccggc gtatcacgcg gttgcctcaa tggggcaatt     1800

tggaaggaga aatataggga aaatcacgtc gctctcggac ggggaagagt tccagactat     1860

gagggggggg ggtggtatat aaagacagga gatgtccacc cccagagaga ggaagaagtt     1920

ggaactttag aagagagaga taactttccc cagtgtccat caatacacaa ccaaacacaa     1980

actctatatt tacacatata accccctctc tagaatgcca cattcctatg actacgatgc     2040

cattgtcatt ggttccggtc caggtggtga aggtgctgca atgggcttag ttaagcaggg     2100

tgctagagtt gctgtcatcg aaagatatca aaatgttggt ggtggttgta ctcactgggg     2160

tacaattcca tctaaggcat tgagacatgc agtttccaga attattgagt ttaaccaaaa     2220

ccctttatac tctgatcatt caagattgtt gagatcatct tttgctgata ttttgaacca     2280

tgctgacaac gtcatcaacc aacaaactcg tatgcgtcaa ggcttctatg agagaaatca     2340

ttgtgagatt ttacaaggta acgctagatt tgtcgatgag catactcttg cattagactg     2400

tccagacggt tccgttgaga ctcttaccgc tgaaaaattc gttattgctt gtggttccag     2460

accataccac ccaaccgatg tcgatttcac tcaccctcgt atctacgatt ccgattctat     2520

tttgtctatg catcatgaac caagacatgt tttgatttat ggtgctggtg ttatcggttg     2580

tgaatatgct tctattttca gaggtatgga tgttaaggtt gacttgatta atacaagaga     2640

cagattatta gctttccttg atcaggaaat gtctgattcc ctttcctacc atttttggaa     2700

ctccggtgtc gtcatcagac acaacgagga atatgaaaag attgaaggtt gtgatgacgg     2760

cgttattatg caccttaagt ctggtaaaaa gttaaaagca gattgcttgt tatatgcaaa     2820

tggtagaacc ggtaacacag actccttggc tttacaaaac attggtttag aaaccgattc     2880

aagaggtcaa ttaaaggtca attcaatgta tcaaactgca caaccacacg tttacgcagt     2940

tggtgacgtt attggttacc cttcattggc atctgccgct tacgatcaag gtagaatcgc     3000

cgctcaagca cttgttaagg gtgaagcaac tgcacactta atcgaagata tccctaccgg     3060

tatctacact atcccagaaa tctcttctgt tggcaagact gaacaacaat taaccgcaat     3120

gaaggttcca tacgaagtcg gtcgtgccca gttcaagcat ttggctagag cacaaattgt     3180

tggtatgaat gttggtactt tgaaaatctt gtttcacaga gaaacaaagg aaatcttggg     3240

cattcactgt ttcggcgaaa gagctgcaga gattattcac atcggtcaag ccattatgga     3300

acaaaaaggc ggtggtaata ccattgaata tttcgttaat accaccttca actacccaac     3360

aatggccgaa gcatatagag tcgctgcttt aaacggttta aacagattgt tttaattaac     3420

atctgaatgt aaaatgaaca ttaaaatgaa ttactaaact ttacgtctac tttacaatct     3480

ataaactttg tttaatcata taacgaaata cactaataca caatcctgta cgtatgtaat     3540

acttttatcc atcaaggatt gagaaaaaaa agtaatgatt ccctgggcca ttaaaactta     3600

gacccccaag cttggatagg tcactctcta ttttcgtttc tcccttccct gatagaaggg     3660

tgatatgtaa ttaagaataa tatataattt tataataaaa gaattcatag cctcatgaaa     3720

tcagccattt gcttttgttc aacgatcttt tgaaattgtt gttgttcttg gtagttaagt     3780

tgatccatct tggcttatgt tgtgtgtatg ttgtagttat tcttagtata ttcctgtcct     3840

gagtttagtg aaacataata tcgccttgaa atgaaaatgc tgaaattcgt cgacatacaa     3900

tttttcaaac tttttttttt tcttggtgca cggacatgtt tttaaaggaa gtactctata     3960

ccagttattc ttcacaaatt taattgctgg agaatagatc ttcaacgctt taataaagta     4020

gtttgtttgt caaggatggc gtcatacaaa gaaagatcag aatcacacac ttcccctgtt     4080

gctaggagac ttttctccat catggaggaa aagaagtcta acctttgtgc atcattggat     4140

attactgaaa ctgaaaagct tctctctatt ttggacacta ttggtcctta catctgtcta     4200

gttaaaacac acatcgatat tgtttctgat tttacgtatg aaggaactgt gttgcctttg     4260

aaggagcttg ccaagaaaca taattttatg atttttgaag atagaaaatt tgctgatatt     4320

ggtaacactg ttaaaaatca atataaatct ggtgtcttcc gtattgccga atgggctgac     4380

atcactaatg cacatggtgt aacgggtgca ggtattgttt ctggcttgaa ggaggcagcc     4440

caagaaacaa ccagtgaacc tagaggtttg ctaatgcttg ctgagttatc atcaaagggt     4500

tctttagcat atggtgaata tacagaaaaa acagtagaaa ttgctaaatc tgataaagag     4560

tttgtcattg gttttattgc gcaacacgat atgggcggta gagaagaagg ttttgactgg     4620

atcattatga ctccaggggt tggtttagat gacaaaggtg atgcacttgg tcaacaatat     4680

agaactgttg atgaagttgt aaagactgga acggatatca taattgttgg tagaggtttg     4740

tacggtcaag gaagagatcc tatagagcaa gctaaaagat accaacaagc tggttggaat     4800

gcttatttaa acagatttaa atgattctta cacaaagatt tgatacatgt acactagttt     4860

aaataagcat gaaaagaatt acacaagcaa aaaaaaaaaa ataaatgagg tactttacgt     4920

tcacctacaa ccaaaaaaac tagatagagt aaaatcttaa gatttagaaa aagttgttta     4980

acaaaggctt tagtatgtga atttttaatg tagcaaagcg ataactaata aacataaaca     5040

aaagtatggt tttctttatc agtcaaatca ttatcgattg attgttccgc gtatctgcag     5100

atagcctcat gaaatcagcc atttgctttt gttcaacgat cttttgaaat tgttgttgtt     5160

cttggtagtt aagttgatcc atcttggctt atgttgtgtg tatgttgtag ttattcttag     5220

tatattcctg tcctgagttt agtgaaacat aatatcgcct tgaaatgaaa atgctgaaat     5280

tcgtcgacat acaatttttc aaactttttt tttttcttgg tgcacggaca tgtttttaaa     5340

ggaagtactc tataccagtt attcttcaca aatttaattg ctggagaata gatcttcaac     5400

gccccggggg atctggatcc gcggccgcaa taacctcagg gagaactttg gcattgtact     5460

ctccattgac gagtccgcca acccattctt gttaaaccta accttgcatt atcacattcc     5520

ctttgacccc ctttagctgc atttccactt gtctacatta agattcatta cacattcttt     5580

ttcgtatttc tcttacctcc ctcccccctc catggatctt atatataaat cttttctata     5640

acaataatat ctactagagt taaacaacaa ttccacttgg catggctgtc tcagcaaatc     5700

tgcttctacc tactgcacgg gtttgcatgt cattgtttct agcagggaat cgtccatgta     5760

cgttgtcctc catgatggtc ttcccgctgc cactttcttt agtatcttaa atagagcaga     5820

tcttacgtcc actgtgcatc cgtgcacccc gaaaatcgta tggttttcct tgccacctct     5880

cacaattttg aatatgctca acgcgaaaga gaggggaaga ggaatcgcat tcgtagagtg     5940

gctacattca accctgacaa aggaactagc gtttgtgcag gagagagtgg tttgcataga     6000

tttcctttcc tttgcaagca tattatatag agtagccaat acagtaacag ctacagcaca     6060

aaaaagagaa cgagaacgag aacgagaaca agaacaagaa ctagcactac tgtcactgcc     6120

agcatcaaca ttactaccat tattccaaca tgtttgcaac tagaaatata accattggtg     6180

tcagaacact cagaccaacc agtttcttga aaacaaggtc ttttctgcaa cagaggctac     6240

aatcaacgct aaagaagagc tatgaaccaa ccaaatccg                            6279


<210>  136
<211>  6888
<212>  DNA
<213>  Artificial

<220>
<223>  A. vinelandii MEL integration fragment


<220>
<221>  misc_feature
<222>  (2914)..(2914)
<223>  n is a, c, g, or t

<400>  136
ccttcattta cgaaataaag tgccgcggtt acgcagcaca caccagcaat cacgtgcagt       60

gtctttttct tttttttttc ttttttttcc tctttttctt ttgttttgtt tcgtttcttt      120

tccgccagtt cccgttttcc atttccggaa caacaatggg actccactgt tttctttccc      180

cccttccgtt ttcggctcgc agtctgtaca tgcacgttta tccgacacct gtcttgtttg      240

gcgcgtaatt aatacagttt ctccggagtc caggtctcgg acgggtaatt tacacgtcat      300

cattcatttc tgtgtcaaga gaggtagcgc aaaaagtaga aatggtgaac cacgggaatg      360

acttgctgga aatcgacgcc agagtccatt tgaaaaccta cctctacaag agaggaaaca      420

cactacaggg tgtccctggt ccgtaaaatg gcgtaatatg atgacttccc tctatagacg      480

ttgtatttcc agctccaaca tggttaaact attgctatgg tgatggtatt acagatagta      540

aaagaaggaa gggggggtgg caatctcacc ctaacagtta ctaagaacgt ctacttcatc      600

tactgtcaat atacattggc cacatgccga gaaattacgt cgacgccaaa gaagggctca      660

gccgaaaaaa gaaatggaaa acttggccga aaagggaaac aaacaaaaag gtgatgtaaa      720

attagcggaa aggggaattg gcaaattgag ggagaaaaaa aaaaggcaga aaaggaggcg      780

gaaagtcagt acgttttgaa ggcgtcattg gttttccctt ttgcagagtg tttcatttct      840

tttgtttcat gacgtagtgg cgtttctttt cctgcacttt agaaatctat cttttcctta      900

tcaagtaaca agcggttggc aaaggtgtat ataaatcaag gaattcgccc ttgttaatta      960

ataacttcgt atagcataca ttatacgaag ttatgaacta gtatgcaatg ttgctaatgc     1020

aatgtatact ccatgatata tacgagaaaa tagttcaaag gcttcagatt atatacctac     1080

aaaaataaaa ctatctcaat acctgatagg gtatactgcc gtttattatt taatcactct     1140

gttataagta tttgagtatt tttccttttt tttttcatga acttcttaac aatcattatt     1200

tttttttttc tcgtcctgct ttgctcaaca ttgagcttaa gccgagggtc tcaacctata     1260

gaaggcgata ccatgagctg gaacagttgt gttaagtata gcatttggag aaagactacc     1320

aattttctgg ccaaacagtc ttgtatcatt cttagacaaa ccgtctttat aagactgctc     1380

tgtagcattg tagagaatac cggtggctgc cttattctgt tcaaggatag cagacgccgt     1440

agagttgtca actctgttgg cccataagtc gtaaatatcc caagtcgatg tcagttcctt     1500

tgaacccaaa ttgctgtcaa agaaaatctc ttccaaggtc gtgttcattg gtcttgctac     1560

gcttcctcca ttcaataaag caaccacttg gtcaccattg tcaagcggac cactccacat     1620

ttgaatttca ccttgtccat attcatcggt gtctgaaaca taatatctcc agactcttgt     1680

ggctggaata ccctttggat cttgattaat tgcgatgaca gaggcttgac tgtagatcga     1740

gtacgaagat gcctttaagt gattcacgtc ggcaccaatg ataagtgggg actttaccat     1800

tgcccacata gagaaatggg ccttttcctc atcgtcagtc aaattaccga ctccgacctc     1860

tagattgtcc agatcgttcc aaccaccaac acctgcattt tgccccattg gagctgcctt     1920

gttaagaata ttcataatag aacaatggaa accggcgtac ttgcaatcat attcgtcacc     1980

gtcacaggga catctgctat ctggacgggt gaactcagca gtaatatctc cgctcattct     2040

ccaagaattg gcgataccag agccccagta aaatgtcaaa tcctgacccc agttacatag     2100

agaatagaaa ataggcctac cagttttatt caaagcatct gacatggcct tgtaacggtg     2160

gtaagaaacg tctggtgtac caaattgacc tttattgtaa caattatcat acttcaagta     2220

gtcaacgcgg ttatttgcaa agaattgagc atcttcttcc tcacgcccca gagacccagg     2280

gtacccagca caggtgtact caccagcaga cgaatacata ccgaaaagaa agctgttatt     2340

atgcaggtgg tctgcaacat ggcccatacc gttgggaaat ttgtgcttgt ctgcaacgag     2400

gaaaccgtcg gaatccctgc cgctagacca acagtcatct aggatgacat acttgtaacc     2460

catatccttt agccccaagt cagaaattct atcagcagtg tctagaagta gctgttcact     2520

gacatcgcag gcaaacgtat tccagctgtc ccaacccatc tgtggggtga gaccaagacc     2580

attgtaactc ggagaaactc cgaaaacacc cttcaaagtg gtgcatgcgg tgagaaagta     2640

gaaagcaaac attgttgttg ttgttgtcgt tgtttttgtt tttgttgtgt tagtataacg     2700

tgtataatta acagtggttg attctatact gttaaatcgg aagaaacggc aaacaagtcc     2760

tctcagcaac atcaactggc aaaagacaaa acccgccttt tatagactcc ccggagtgtc     2820

gaaatatgac ttcccggcaa cgagttctcc gggtggggtg atagcgggtc ctttcgctac     2880

ctattttccc cccccccccc catcctccat cctncatgcc ccatactcca tgccccctcg     2940

aggatttaca gtgggggcct cacttctcgc ggcactctct tccagaaatt gcacgattac     3000

ccaatatggt ccttcaggag tgacacaacc tgaaaggtgg cggcgcaaaa tgtattgaca     3060

gagcctattg gtggagccta ttgggagcag ctatagtaaa gaggagtagt agacagtagt     3120

agacaatagt agggagtagt tgaagggtgt caattttgaa tatcgtgagt gcattaacta     3180

actatagaag ctaagtagca taaataaata aatataaata taaatataga tataaatgta     3240

ggtgtgtaaa cgtggacatt gatgttgccg ttgcagcata acttcgtata gcatacatta     3300

tacgaagtta tccctgcagg taagggcgaa ttcttttatt ataaaattat atattattct     3360

taattacata tcacccttct atcagggaag ggagaaacga aaatagagag tgacctatcc     3420

aagcttgggg gtctaagttt taatggccca gggaatcatt actttttttt ctcaatcctt     3480

gatggataaa agtattacat acgtacagga ttgtgtatta gtgtatttcg ttatatgatt     3540

aaacaaagtt tatagattgt aaagtagacg taaagtttag taattcattt taatgttcat     3600

tttacattca gatgttaatt aattagaaca atctgttcaa accatcgtac gcagccactc     3660

tatatgcttc tgccattgta ggataattga atgtagtatt aatgaaatac ttcaaagtgt     3720

tggcttcacc cttttggttc ataattgctt gaccgatgtg cacaatttca gatgcttgat     3780

aaccaaaaca atggacgcct aagatttcga gtgtttctct gtgaaacaaa atctttaaca     3840

tgcctgcctt ttcaacagca atctgggctc ttgccatacc tttgaaaaag gccttaccaa     3900

cctcgtatgg aacttttgcc tgagtaagtt ctctttctgt cttaccaaca ctggaaattt     3960

ctggaatggt gtagataccg gtagggacat cgtcaacaaa tctccaggag tcgttttcag     4020

tgatggagcc agcagcggat ctaccttgat cgtaagcagc agatgccaaa gatggccaac     4080

caataacatc accagcagcg tagatgttgg aaacttcggt tctataatgc tcatcgactt     4140

gaatttgacc tctaccatta gcctttaaac caatgttttc aagacccaac ttatcagtgt     4200

taccggttct accgttggac caaaggaatg catctgcctt gatcttttta ccagacttca     4260

aatgtaaaat gacaccgttg tcgaggcctt caactctttc gtactcctcg ttatgtctga     4320

ttaagacatt gttattcctt aagtgataag acaatgagtc ggaaatttcg tcatcgagaa     4380

aagatagtaa ttgatcacga ttgtcaatca aatcgactaa aacacctagt cctgagaaaa     4440

tacttgcgta ttcgcaaccg ataacaccag ctccgtagat aatcaaacgt cttggagtat     4500

gacccaaaga taagatagta tcagaatcgt agatcctagg gtgagtaaag tccacatcgg     4560

ctggtctata aggtcttgaa ccggtagcaa taacaaattg ctttgcgacc aaggtttcga     4620

ccataccatt caaatgaacg acttcaatag tatgctcgtc acagaatgat gcggtaccga     4680

aaaaggtatc aattctgttt ctagcataat atccggttct ggaagagact tgcttcgcaa     4740

taacttgctc tgctgatttt agaacgtcag cgaaagagaa ccatcttggt tcaccaattt     4800

gacggaataa tgggttgttg ttgtactgca taatttgtct aacagagtgc cttaaagcct     4860

tacttgggat agtgcctaag tgcgtacaat taccgccaac ttgtggtcta tcatcaacaa     4920

ctgcaacttt tctaccagct ttgactgcat tcatagcggc accttcaccc gctggacctg     4980

taccgataac aacaacatca taattgtaaa cagccattct agagaggggg ttatatgtgt     5040

aaatatagag tttgtgtttg gttgtgtatt gatggacact ggggaaagtt atctctctct     5100

tctaaagttc caacttcttc ctctctctgg gggtggacat ctcctgtctt tatataccac     5160

ccccccccct catagtctgg aactcttccc cgtccgagag cgacgtgatt ttccctatat     5220

ttctccttcc aaattgcccc attgaggcaa ccgcgtgata cgccgggtct tttcttcccg     5280

ggagtggaga cagttcccaa gtggtaaagt ccacttccat catgatccac ctccatgaca     5340

ctcttttaaa cacatgtgct gcacatgtgt tggtgtggtt acacgatata caaccatgtt     5400

gggcaaccac aggtgcgcag catcaacaac ggggcgagtc aggaggaacg gtaaccttgg     5460

cggtttacga atgaggtgtt ccccgttgga gaggtgtcta tggacgacca tcccagtaca     5520

atggacgggt tggttggtgt ggttacggtg tcaacgacag agacatgggc caacagagac     5580

attatggaga gacgttggga gtggatcctc cctggaaagg gtgtctttgg cgaagtgttg     5640

ttgatttgta tgtctgggtc ggttgtttgt tgtgctagtt gctgcataat ggtaccaaca     5700

ccagcggttt ttttgtcctc ctctgcaaca ggggaactcg gccacttgtt tattgggggg     5760

gggggggggg atgccacgtg gtaatgaaac attatcaaga gacctctctt tgtaggggta     5820

cctgtgatgt gtgtgtgtgt gcatgtgcat agatgcatat tgagacagtt gtgttcattg     5880

actgtctgca tttctccaga accaataaaa aataaaaata gtagcaatac ctacactttc     5940

tggaccttgt agtaacgtag acgccgtaag gttgcatttc acgccagctt gagaggggga     6000

aagaactacc aatctcgagg gatccgcggc cgcaataacc tcagggagaa ctttggcatt     6060

gtactctcca ttgacgagtc cgccaaccca ttcttgttaa acctaacctt gcattatcac     6120

attccctttg acccccttta gctgcatttc cacttgtcta cattaagatt cattacacat     6180

tctttttcgt atttctctta cctccctccc ccctccatgg atcttatata taaatctttt     6240

ctataacaat aatatctact agagttaaac aacaattcca cttggcatgg ctgtctcagc     6300

aaatctgctt ctacctactg cacgggtttg catgtcattg tttctagcag ggaatcgtcc     6360

atgtacgttg tcctccatga tggtcttccc gctgccactt tctttagtat cttaaataga     6420

gcagatctta cgtccactgt gcatccgtgc accccgaaaa tcgtatggtt ttccttgcca     6480

cctctcacaa ttttgaatat gctcaacgcg aaagagaggg gaagaggaat cgcattcgta     6540

gagtggctac attcaaccct gacaaaggaa ctagcgtttg tgcaggagag agtggtttgc     6600

atagatttcc tttcctttgc aagcatatta tatagagtag ccaatacagt aacagctaca     6660

gcacaaaaaa gagaacgaga acgagaacga gaacaagaac aagaactagc actactgtca     6720

ctgccagcat caacattact accattattc caacatgttt gcaactagaa atataaccat     6780

tggtgtcaga acactcagac caaccagttt cttgaaaaca aggtcttttc tgcaacagag     6840

gctacaatca acgctaaaga agagctatga accaaccaaa tccgagct                  6888


<210>  137
<211>  6276
<212>  DNA
<213>  Artificial

<220>
<223>  A. vinelandii URA integration fragment

<400>  137
ggccccttca tttacgaaat aaagtgccgc ggttacgcag cacacaccag caatcacgtg       60

cagtgtcttt ttcttttttt tttctttttt ttcctctttt tcttttgttt tgtttcgttt      120

cttttccgcc agttcccgtt ttccatttcc ggaacaacaa tgggactcca ctgttttctt      180

tccccccttc cgttttcggc tcgcagtctg tacatgcacg tttatccgac acctgtcttg      240

tttggcgcgt aattaataca gtttctccgg agtccaggtc tcggacgggt aatttacacg      300

tcatcattca tttctgtgtc aagagaggta gcgcaaaaag tagaaatggt gaaccacggg      360

aatgacttgc tggaaatcga cgccagagtc catttgaaaa cctacctcta caagagagga      420

aacacactac agggtgtccc tggtccgtaa aatggcgtaa tatgatgact tccctctata      480

gacgttgtat ttccagctcc aacatggtta aactattgct atggtgatgg tattacagat      540

agtaaaagaa ggaagggggg gtggcaatct caccctaaca gttactaaga acgtctactt      600

catctactgt caatatacat tggccacatg ccgagaaatt acgtcgacgc caaagaaggg      660

ctcagccgaa aaaagaaatg gaaaacttgg ccgaaaaggg aaacaaacaa aaaggtgatg      720

taaaattagc ggaaagggga attggcaaat tgagggagaa aaaaaaaagg cagaaaagga      780

ggcggaaagt cagtacgttt tgaaggcgtc attggttttc ccttttgcag agtgtttcat      840

ttcttttgtt tcatgacgta gtggcgtttc ttttcctgca ctttagaaat ctatcttttc      900

cttatcaagt aacaagcggt tggcaaaggt gtatataaat caaggaattc ccactttgaa      960

ccctttgaat tttgatatcg tttattttaa atttatttgc ggccgcggat ccctcgagat     1020

tggtagttct ttccccctct caagctggcg tgaaatgcaa ccttacggcg tctacgttac     1080

tacaaggtcc agaaagtgta ggtattgcta ctatttttat tttttattgg ttctggagaa     1140

atgcagacag tcaatgaaca caactgtctc aatatgcatc tatgcacatg cacacacaca     1200

cacatcacag gtacccctac aaagagaggt ctcttgataa tgtttcatta ccacgtggca     1260

tccccccccc cccccccaat aaacaagtgg ccgagttccc ctgttgcaga ggaggacaaa     1320

aaaaccgctg gtgttggtac cattatgcag caactagcac aacaaacaac cgacccagac     1380

atacaaatca acaacacttc gccaaagaca ccctttccag ggaggatcca ctcccaacgt     1440

ctctccataa tgtctctgtt ggcccatgtc tctgtcgttg acaccgtaac cacaccaacc     1500

aacccgtcca ttgtactggg atggtcgtcc atagacacct ctccaacggg gaacacctca     1560

ttcgtaaacc gccaaggtta ccgttcctcc tgactcgccc cgttgttgat gctgcgcacc     1620

tgtggttgcc caacatggtt gtatatcgtg taaccacacc aacacatgtg cagcacatgt     1680

gtttaaaaga gtgtcatgga ggtggatcat gatggaagtg gactttacca cttgggaact     1740

gtctccactc ccgggaagaa aagacccggc gtatcacgcg gttgcctcaa tggggcaatt     1800

tggaaggaga aatataggga aaatcacgtc gctctcggac ggggaagagt tccagactat     1860

gagggggggg ggtggtatat aaagacagga gatgtccacc cccagagaga ggaagaagtt     1920

ggaactttag aagagagaga taactttccc cagtgtccat caatacacaa ccaaacacaa     1980

actctatatt tacacatata accccctctc tagaatggct gtttacaatt atgatgttgt     2040

tgttatcggt acaggtccag cgggtgaagg tgccgctatg aatgcagtca aagctggtag     2100

aaaagttgca gttgttgatg atagaccaca agttggcggt aattgtacgc acttaggcac     2160

tatcccaagt aaggctttaa ggcactctgt tagacaaatt atgcagtaca acaacaaccc     2220

attattccgt caaattggtg aaccaagatg gttctctttc gctgacgttc taaaatcagc     2280

agagcaagtt attgcgaagc aagtctcttc cagaaccgga tattatgcta gaaacagaat     2340

tgataccttt ttcggtaccg catcattctg tgacgagcat actattgaag tcgttcattt     2400

gaatggtatg gtcgaaacct tggtcgcaaa gcaatttgtt attgctaccg gttcaagacc     2460

ttatagacca gccgatgtgg actttactca ccctaggatc tacgattctg atactatctt     2520

atctttgggt catactccaa gacgtttgat tatctacgga gctggtgtta tcggttgcga     2580

atacgcaagt attttctcag gactaggtgt tttagtcgat ttgattgaca atcgtgatca     2640

attactatct tttctcgatg acgaaatttc cgactcattg tcttatcact taaggaataa     2700

caatgtctta atcagacata acgaggagta cgaaagagtt gaaggcctcg acaacggtgt     2760

cattttacat ttgaagtctg gtaaaaagat caaggcagat gcattccttt ggtccaacgg     2820

tagaaccggt aacactgata agttgggtct tgaaaacatt ggtttaaagg ctaatggtag     2880

aggtcaaatt caagtcgatg agcattatag aaccgaagtt tccaacatct acgctgctgg     2940

tgatgttatt ggttggccat ctttggcatc tgctgcttac gatcaaggta gatccgctgc     3000

tggctccatc actgaaaacg actcctggag atttgttgac gatgtcccta ccggtatcta     3060

caccattcca gaaatttcca gtgttggtaa gacagaaaga gaacttactc aggcaaaagt     3120

tccatacgag gttggtaagg cctttttcaa aggtatggca agagcccaga ttgctgttga     3180

aaaggcaggc atgttaaaga ttttgtttca cagagaaaca ctcgaaatct taggcgtcca     3240

ttgttttggt tatcaagcat ctgaaattgt gcacatcggt caagcaatta tgaaccaaaa     3300

gggtgaagcc aacactttga agtatttcat taatactaca ttcaattatc ctacaatggc     3360

agaagcatat agagtggctg cgtacgatgg tttgaacaga ttgttctaat taattaacat     3420

ctgaatgtaa aatgaacatt aaaatgaatt actaaacttt acgtctactt tacaatctat     3480

aaactttgtt taatcatata acgaaataca ctaatacaca atcctgtacg tatgtaatac     3540

ttttatccat caaggattga gaaaaaaaag taatgattcc ctgggccatt aaaacttaga     3600

cccccaagct tggataggtc actctctatt ttcgtttctc ccttccctga tagaagggtg     3660

atatgtaatt aagaataata tataatttta taataaaaga attcatagcc tcatgaaatc     3720

agccatttgc ttttgttcaa cgatcttttg aaattgttgt tgttcttggt agttaagttg     3780

atccatcttg gcttatgttg tgtgtatgtt gtagttattc ttagtatatt cctgtcctga     3840

gtttagtgaa acataatatc gccttgaaat gaaaatgctg aaattcgtcg acatacaatt     3900

tttcaaactt tttttttttc ttggtgcacg gacatgtttt taaaggaagt actctatacc     3960

agttattctt cacaaattta attgctggag aatagatctt caacgcttta ataaagtagt     4020

ttgtttgtca aggatggcgt catacaaaga aagatcagaa tcacacactt cccctgttgc     4080

taggagactt ttctccatca tggaggaaaa gaagtctaac ctttgtgcat cattggatat     4140

tactgaaact gaaaagcttc tctctatttt ggacactatt ggtccttaca tctgtctagt     4200

taaaacacac atcgatattg tttctgattt tacgtatgaa ggaactgtgt tgcctttgaa     4260

ggagcttgcc aagaaacata attttatgat ttttgaagat agaaaatttg ctgatattgg     4320

taacactgtt aaaaatcaat ataaatctgg tgtcttccgt attgccgaat gggctgacat     4380

cactaatgca catggtgtaa cgggtgcagg tattgtttct ggcttgaagg aggcagccca     4440

agaaacaacc agtgaaccta gaggtttgct aatgcttgct gagttatcat caaagggttc     4500

tttagcatat ggtgaatata cagaaaaaac agtagaaatt gctaaatctg ataaagagtt     4560

tgtcattggt tttattgcgc aacacgatat gggcggtaga gaagaaggtt ttgactggat     4620

cattatgact ccaggggttg gtttagatga caaaggtgat gcacttggtc aacaatatag     4680

aactgttgat gaagttgtaa agactggaac ggatatcata attgttggta gaggtttgta     4740

cggtcaagga agagatccta tagagcaagc taaaagatac caacaagctg gttggaatgc     4800

ttatttaaac agatttaaat gattcttaca caaagatttg atacatgtac actagtttaa     4860

ataagcatga aaagaattac acaagcaaaa aaaaaaaaat aaatgaggta ctttacgttc     4920

acctacaacc aaaaaaacta gatagagtaa aatcttaaga tttagaaaaa gttgtttaac     4980

aaaggcttta gtatgtgaat ttttaatgta gcaaagcgat aactaataaa cataaacaaa     5040

agtatggttt tctttatcag tcaaatcatt atcgattgat tgttccgcgt atctgcagat     5100

agcctcatga aatcagccat ttgcttttgt tcaacgatct tttgaaattg ttgttgttct     5160

tggtagttaa gttgatccat cttggcttat gttgtgtgta tgttgtagtt attcttagta     5220

tattcctgtc ctgagtttag tgaaacataa tatcgccttg aaatgaaaat gctgaaattc     5280

gtcgacatac aatttttcaa actttttttt tttcttggtg cacggacatg tttttaaagg     5340

aagtactcta taccagttat tcttcacaaa tttaattgct ggagaataga tcttcaacgc     5400

cccgggggat ctggatccgc ggccgcaata acctcaggga gaactttggc attgtactct     5460

ccattgacga gtccgccaac ccattcttgt taaacctaac cttgcattat cacattccct     5520

ttgaccccct ttagctgcat ttccacttgt ctacattaag attcattaca cattcttttt     5580

cgtatttctc ttacctccct cccccctcca tggatcttat atataaatct tttctataac     5640

aataatatct actagagtta aacaacaatt ccacttggca tggctgtctc agcaaatctg     5700

cttctaccta ctgcacgggt ttgcatgtca ttgtttctag cagggaatcg tccatgtacg     5760

ttgtcctcca tgatggtctt cccgctgcca ctttctttag tatcttaaat agagcagatc     5820

ttacgtccac tgtgcatccg tgcaccccga aaatcgtatg gttttccttg ccacctctca     5880

caattttgaa tatgctcaac gcgaaagaga ggggaagagg aatcgcattc gtagagtggc     5940

tacattcaac cctgacaaag gaactagcgt ttgtgcagga gagagtggtt tgcatagatt     6000

tcctttcctt tgcaagcata ttatatagag tagccaatac agtaacagct acagcacaaa     6060

aaagagaacg agaacgagaa cgagaacaag aacaagaact agcactactg tcactgccag     6120

catcaacatt actaccatta ttccaacatg tttgcaacta gaaatataac cattggtgtc     6180

agaacactca gaccaaccag tttcttgaaa acaaggtctt ttctgcaaca gaggctacaa     6240

tcaacgctaa agaagagcta tgaaccaacc aaatcc                               6276


<210>  138
<211>  6892
<212>  DNA
<213>  Artificial

<220>
<223>  Pseudomonas fluoresens SthA MEL integration fragment


<220>
<221>  misc_feature
<222>  (3975)..(3975)
<223>  n is a, c, g, or t

<400>  138
cggatttggt tggttcatag ctcttcttta gcgttgattg tagcctctgt tgcagaaaag       60

accttgtttt caagaaactg gttggtctga gtgttctgac accaatggtt atatttctag      120

ttgcaaacat gttggaataa tggtagtaat gttgatgctg gcagtgacag tagtgctagt      180

tcttgttctt gttctcgttc tcgttctcgt tctctttttt gtgctgtagc tgttactgta      240

ttggctactc tatataatat gcttgcaaag gaaaggaaat ctatgcaaac cactctctcc      300

tgcacaaacg ctagttcctt tgtcagggtt gaatgtagcc actctacgaa tgcgattcct      360

cttcccctct ctttcgcgtt gagcatattc aaaattgtga gaggtggcaa ggaaaaccat      420

acgattttcg gggtgcacgg atgcacagtg gacgtaagat ctgctctatt taagatacta      480

aagaaagtgg cagcgggaag accatcatgg aggacaacgt acatggacga ttccctgcta      540

gaaacaatga catgcaaacc cgtgcagtag gtagaagcag atttgctgag acagccatgc      600

caagtggaat tgttgtttaa ctctagtaga tattattgtt atagaaaaga tttatatata      660

agatccatgg aggggggagg gaggtaagag aaatacgaaa aagaatgtgt aatgaatctt      720

aatgtagaca agtggaaatg cagctaaagg gggtcaaagg gaatgtgata atgcaaggtt      780

aggtttaaca agaatgggtt ggcggactcg tcaatggaga gtacaatgcc aaagttctcc      840

ctgaggttat tgcggccgcg gatccctcga gattggtagt tctttccccc tctcaagctg      900

gcgtgaaatg caaccttacg gcgtctacgt tactacaagg tccagaaagt gtaggtattg      960

ctactatttt tattttttat tggttctgga gaaatgcaga cagtcaatga acacaactgt     1020

ctcaatatgc atctatgcac atgcacacac acacacatca caggtacccc tacaaagaga     1080

ggtctcttga taatgtttca ttaccacgtg gcatcccccc cccccccccc aataaacaag     1140

tggccgagtt cccctgttgc agaggaggac aaaaaaaccg ctggtgttgg taccattatg     1200

cagcaactag cacaacaaac aaccgaccca gacatacaaa tcaacaacac ttcgccaaag     1260

acaccctttc cagggaggat ccactcccaa cgtctctcca taatgtctct gttggcccat     1320

gtctctgtcg ttgacaccgt aaccacacca accaacccgt ccattgtact gggatggtcg     1380

tccatagaca cctctccaac ggggaacacc tcattcgtaa accgccaagg ttaccgttcc     1440

tcctgactcg ccccgttgtt gatgctgcgc acctgtggtt gcccaacatg gttgtatatc     1500

gtgtaaccac accaacacat gtgcagcaca tgtgtttaaa agagtgtcat ggaggtggat     1560

catgatggaa gtggacttta ccacttggga actgtctcca ctcccgggaa gaaaagaccc     1620

ggcgtatcac gcggttgcct caatggggca atttggaagg agaaatatag ggaaaatcac     1680

gtcgctctcg gacggggaag agttccagac tatgaggggg gggggtggta tataaagaca     1740

ggagatgtcc acccccagag agaggaagaa gttggaactt tagaagagag agataacttt     1800

ccccagtgtc catcaataca caaccaaaca caaactctat atttacacat ataaccccct     1860

ctctagataa aatggctgtt tataactacg acgttgttgt tttgggttct ggtccagcag     1920

gcgaaggtgc tgctatgaat gcagctaaag caggcagaaa agttgctatg gttgattcac     1980

gtagacaagt cggtggtaac tgtacccact taggtactat tccttctaag gctttgagac     2040

actctgttcg tcaaatcatg caattcaaca ctaatccaat gttcagagcc attggcgaac     2100

caagatggtt ctcctttcca gatgttttaa agtctgcaga aaaggttatt tccaagcaag     2160

tcgcttctcg taccggctat tacgctagaa acagagttga tttgtttttc ggtactggtt     2220

ccttcgcaga tgaacagact gttgaagtcg tttgtgcaaa tggtgttgtc gagaagttag     2280

ttgctaagca tattatcatc gccacaggtt ccagacctta cagaccagca gacatcgatt     2340

tccatcatcc acgtatctac gactctgata ccatcttatc tttaggccac acccctagaa     2400

agttgattat ctacggtgcc ggtgttatcg gttgcgagta tgcttctatc ttttcaggtt     2460

tgggtgtctt agtcgagttg gtcgataaca gagatcaact tttgtccttt ttagactctg     2520

aaatttctca agctctttcc tatcactttt ctaataacaa cattacagtt agacataatg     2580

aggaatacga cagagtcgaa ggtttagata acggtgttat tttgcatttg aagtccggta     2640

aaaagattaa ggccgatgca ttgttatggt gtaacggtag aactggtaat actgacaagt     2700

taggtatgga aaacattggt gttaaggtca actccagagg tcaaattgaa gttgacgaga     2760

attacagaac ctgtgtcaca aacatttatg gtgctggtga tgttattggt tggccatcac     2820

ttgcctcagc agctcacgac caaggtagat cagcagctgg ctctatcgtt gataatggtt     2880

cctggagata tgtcaacgat gttccaaccg gtatctacac tattccagaa atttcctcaa     2940

ttggtaaaaa tgaacacgaa ttgactaaag ctaaggttcc ttatgaggtc ggtaaagcct     3000

ttttcaagtc tatggcaaga gcacaaattg ctggtgaacc acagggtatg cttaaaatct     3060

tattccatag agaaacttta gaagtcttag gtgttcactg ttttggttat caagcatccg     3120

aaattgttca tattggccag gcaattatga accaaccagg tgaacaaaat actcttaagt     3180

acttcgtcaa taccaccttc aactacccaa caatggctga agcatataga gttgcagctt     3240

acgatggttt gaacagattg ttctaattaa ttaacatctg aatgtaaaat gaacattaaa     3300

atgaattact aaactttacg tctactttac aatctataaa ctttgtttaa tcatataacg     3360

aaatacacta atacacaatc ctgtacgtat gtaatacttt tatccatcaa ggattgagaa     3420

aaaaaagtaa tgattccctg ggccattaaa acttagaccc ccaagcttgg ataggtcact     3480

ctctattttc gtttctccct tccctgatag aagggtgata tgtaattaag aataatatat     3540

aattttataa taaaagaatt cgcccttacc tgcagggata acttcgtata atgtatgcta     3600

tacgaagtta tgctgcaacg gcaacatcaa tgtccacgtt tacacaccta catttatatc     3660

tatatttata tttatattta tttatttatg ctacttagct tctatagtta gttaatgcac     3720

tcacgatatt caaaattgac acccttcaac tactccctac tattgtctac tactgtctac     3780

tactcctctt tactatagct gctcccaata ggctccacca ataggctctg tcaatacatt     3840

ttgcgccgcc acctttcagg ttgtgtcact cctgaaggac catattgggt aatcgtgcaa     3900

tttctggaag agagtgccgc gagaagtgag gcccccactg taaatcctcg agggggcatg     3960

gagtatgggg catgnaggat ggaggatggg gggggggggg gaaaataggt agcgaaagga     4020

cccgctatca ccccacccgg agaactcgtt gccgggaagt catatttcga cactccgggg     4080

agtctataaa aggcgggttt tgtcttttgc cagttgatgt tgctgagagg acttgtttgc     4140

cgtttcttcc gatttaacag tatagaatca accactgtta attatacacg ttatactaac     4200

acaacaaaaa caaaaacaac gacaacaaca acaacaatgt ttgctttcta ctttctcacc     4260

gcatgcacca ctttgaaggg tgttttcgga gtttctccga gttacaatgg tcttggtctc     4320

accccacaga tgggttggga cagctggaat acgtttgcct gcgatgtcag tgaacagcta     4380

cttctagaca ctgctgatag aatttctgac ttggggctaa aggatatggg ttacaagtat     4440

gtcatcctag atgactgttg gtctagcggc agggattccg acggtttcct cgttgcagac     4500

aagcacaaat ttcccaacgg tatgggccat gttgcagacc acctgcataa taacagcttt     4560

cttttcggta tgtattcgtc tgctggtgag tacacctgtg ctgggtaccc tgggtctctg     4620

gggcgtgagg aagaagatgc tcaattcttt gcaaataacc gcgttgacta cttgaagtat     4680

gataattgtt acaataaagg tcaatttggt acaccagacg tttcttacca ccgttacaag     4740

gccatgtcag atgctttgaa taaaactggt aggcctattt tctattctct atgtaactgg     4800

ggtcaggatt tgacatttta ctggggctct ggtatcgcca attcttggag aatgagcgga     4860

gatattactg ctgagttcac ccgtccagat agcagatgtc cctgtgacgg tgacgaatat     4920

gattgcaagt acgccggttt ccattgttct attatgaata ttcttaacaa ggcagctcca     4980

atggggcaaa atgcaggtgt tggtggttgg aacgatctgg acaatctaga ggtcggagtc     5040

ggtaatttga ctgacgatga ggaaaaggcc catttctcta tgtgggcaat ggtaaagtcc     5100

ccacttatca ttggtgccga cgtgaatcac ttaaaggcat cttcgtactc gatctacagt     5160

caagcctctg tcatcgcaat taatcaagat ccaaagggta ttccagccac aagagtctgg     5220

agatattatg tttcagacac cgatgaatat ggacaaggtg aaattcaaat gtggagtggt     5280

ccgcttgaca atggtgacca agtggttgct ttattgaatg gaggaagcgt agcaagacca     5340

atgaacacga ccttggaaga gattttcttt gacagcaatt tgggttcaaa ggaactgaca     5400

tcgacttggg atatttacga cttatgggcc aacagagttg acaactctac ggcgtctgct     5460

atccttgaac agaataaggc agccaccggt attctctaca atgctacaga gcagtcttat     5520

aaagacggtt tgtctaagaa tgatacaaga ctgtttggcc agaaaattgg tagtctttct     5580

ccaaatgcta tacttaacac aactgttcca gctcatggta tcgccttcta taggttgaga     5640

ccctcggctt aagctcaatg ttgagcaaag caggacgaga aaaaaaaaaa taatgattgt     5700

taagaagttc atgaaaaaaa aaaggaaaaa tactcaaata cttataacag agtgattaaa     5760

taataaacgg cagtataccc tatcaggtat tgagatagtt ttatttttgt aggtatataa     5820

tctgaagcct ttgaactatt ttctcgtata tatcatggag tatacattgc attagcaaca     5880

ttgcatacta gttcataact tcgtataatg tatgctatac gaagttatta attaacaagg     5940

gcgaattcct tgatttatat acacctttgc caaccgcttg ttacttgata aggaaaagat     6000

agatttctaa agtgcaggaa aagaaacgcc actacgtcat gaaacaaaag aaatgaaaca     6060

ctctgcaaaa gggaaaacca atgacgcctt caaaacgtac tgactttccg cctccttttc     6120

tgcctttttt ttttctccct caatttgcca attccccttt ccgctaattt tacatcacct     6180

ttttgtttgt ttcccttttc ggccaagttt tccatttctt ttttcggctg agcccttctt     6240

tggcgtcgac gtaatttctc ggcatgtggc caatgtatat tgacagtaga tgaagtagac     6300

gttcttagta actgttaggg tgagattgcc accccccctt ccttctttta ctatctgtaa     6360

taccatcacc atagcaatag tttaaccatg ttggagctgg aaatacaacg tctatagagg     6420

gaagtcatca tattacgcca ttttacggac cagggacacc ctgtagtgtg tttcctctct     6480

tgtagaggta ggttttcaaa tggactctgg cgtcgatttc cagcaagtca ttcccgtggt     6540

tcaccatttc tactttttgc gctacctctc ttgacacaga aatgaatgat gacgtgtaaa     6600

ttacccgtcc gagacctgga ctccggagaa actgtattaa ttacgcgcca aacaagacag     6660

gtgtcggata aacgtgcatg tacagactgc gagccgaaaa cggaaggggg gaaagaaaac     6720

agtggagtcc cattgttgtt ccggaaatgg aaaacgggaa ctggcggaaa agaaacgaaa     6780

caaaacaaaa gaaaaagagg aaaaaaaaga aaaaaaaaag aaaaagacac tgcacgtgat     6840

tgctggtgtg tgctgcgtaa ccgcggcact ttatttcgta aatgaagggg cc             6892


<210>  139
<211>  1395
<212>  DNA
<213>  Pseudomonas fluorescens

<400>  139
ttagaacaat ctgttcaaac catcgtaagc tgcaactcta tatgcttcag ccattgttgg       60

gtagttgaag gtggtattga cgaagtactt aagagtattt tgttcacctg gttggttcat      120

aattgcctgg ccaatatgaa caatttcgga tgcttgataa ccaaaacagt gaacacctaa      180

gacttctaaa gtttctctat ggaataagat tttaagcata ccctgtggtt caccagcaat      240

ttgtgctctt gccatagact tgaaaaaggc tttaccgacc tcataaggaa ccttagcttt      300

agtcaattcg tgttcatttt taccaattga ggaaatttct ggaatagtgt agataccggt      360

tggaacatcg ttgacatatc tccaggaacc attatcaacg atagagccag ctgctgatct      420

accttggtcg tgagctgctg aggcaagtga tggccaacca ataacatcac cagcaccata      480

aatgtttgtg acacaggttc tgtaattctc gtcaacttca atttgacctc tggagttgac      540

cttaacacca atgttttcca tacctaactt gtcagtatta ccagttctac cgttacacca      600

taacaatgca tcggccttaa tctttttacc ggacttcaaa tgcaaaataa caccgttatc      660

taaaccttcg actctgtcgt attcctcatt atgtctaact gtaatgttgt tattagaaaa      720

gtgataggaa agagcttgag aaatttcaga gtctaaaaag gacaaaagtt gatctctgtt      780

atcgaccaac tcgactaaga cacccaaacc tgaaaagata gaagcatact cgcaaccgat      840

aacaccggca ccgtagataa tcaactttct aggggtgtgg cctaaagata agatggtatc      900

agagtcgtag atacgtggat gatggaaatc gatgtctgct ggtctgtaag gtctggaacc      960

tgtggcgatg ataatatgct tagcaactaa cttctcgaca acaccatttg cacaaacgac     1020

ttcaacagtc tgttcatctg cgaaggaacc agtaccgaaa aacaaatcaa ctctgtttct     1080

agcgtaatag ccggtacgag aagcgacttg cttggaaata accttttctg cagactttaa     1140

aacatctgga aaggagaacc atcttggttc gccaatggct ctgaacattg gattagtgtt     1200

gaattgcatg atttgacgaa cagagtgtct caaagcctta gaaggaatag tacctaagtg     1260

ggtacagtta ccaccgactt gtctacgtga atcaaccata gcaacttttc tgcctgcttt     1320

agctgcattc atagcagcac cttcgcctgc tggaccagaa cccaaaacaa caacgtcgta     1380

gttataaaca gccat                                                      1395


<210>  140
<211>  6281
<212>  DNA
<213>  Artificial

<220>
<223>  Pseudomonas fluorescens URA integration fragment

<400>  140
cggatttggt tggttcatag ctcttcttta gcgttgattg tagcctctgt tgcagaaaag       60

accttgtttt caagaaactg gttggtctga gtgttctgac accaatggtt atatttctag      120

ttgcaaacat gttggaataa tggtagtaat gttgatgctg gcagtgacag tagtgctagt      180

tcttgttctt gttctcgttc tcgttctcgt tctctttttt gtgctgtagc tgttactgta      240

ttggctactc tatataatat gcttgcaaag gaaaggaaat ctatgcaaac cactctctcc      300

tgcacaaacg ctagttcctt tgtcagggtt gaatgtagcc actctacgaa tgcgattcct      360

cttcccctct ctttcgcgtt gagcatattc aaaattgtga gaggtggcaa ggaaaaccat      420

acgattttcg gggtgcacgg atgcacagtg gacgtaagat ctgctctatt taagatacta      480

aagaaagtgg cagcgggaag accatcatgg aggacaacgt acatggacga ttccctgcta      540

gaaacaatga catgcaaacc cgtgcagtag gtagaagcag atttgctgag acagccatgc      600

caagtggaat tgttgtttaa ctctagtaga tattattgtt atagaaaaga tttatatata      660

agatccatgg aggggggagg gaggtaagag aaatacgaaa aagaatgtgt aatgaatctt      720

aatgtagaca agtggaaatg cagctaaagg gggtcaaagg gaatgtgata atgcaaggtt      780

aggtttaaca agaatgggtt ggcggactcg tcaatggaga gtacaatgcc aaagttctcc      840

ctgaggttat tgcggccgcg gatccagatc ccccggggcg ttgaagatct attctccagc      900

aattaaattt gtgaagaata actggtatag agtacttcct ttaaaaacat gtccgtgcac      960

caagaaaaaa aaaaagtttg aaaaattgta tgtcgacgaa tttcagcatt ttcatttcaa     1020

ggcgatatta tgtttcacta aactcaggac aggaatatac taagaataac tacaacatac     1080

acacaacata agccaagatg gatcaactta actaccaaga acaacaacaa tttcaaaaga     1140

tcgttgaaca aaagcaaatg gctgatttca tgaggctatc tgcagatacg cggaacaatc     1200

aatcgataat gatttgactg ataaagaaaa ccatactttt gtttatgttt attagttatc     1260

gctttgctac attaaaaatt cacatactaa agcctttgtt aaacaacttt ttctaaatct     1320

taagatttta ctctatctag tttttttggt tgtaggtgaa cgtaaagtac ctcatttatt     1380

tttttttttt tgcttgtgta attcttttca tgcttattta aactagtgta catgtatcaa     1440

atctttgtgt aagaatcatt taaatctgtt taaataagca ttccaaccag cttgttggta     1500

tcttttagct tgctctatag gatctcttcc ttgaccgtac aaacctctac caacaattat     1560

gatatccgtt ccagtcttta caacttcatc aacagttcta tattgttgac caagtgcatc     1620

acctttgtca tctaaaccaa cccctggagt cataatgatc cagtcaaaac cttcttctct     1680

accgcccata tcgtgttgcg caataaaacc aatgacaaac tctttatcag atttagcaat     1740

ttctactgtt ttttctgtat attcaccata tgctaaagaa ccctttgatg ataactcagc     1800

aagcattagc aaacctctag gttcactggt tgtttcttgg gctgcctcct tcaagccaga     1860

aacaatacct gcacccgtta caccatgtgc attagtgatg tcagcccatt cggcaatacg     1920

gaagacacca gatttatatt gatttttaac agtgttacca atatcagcaa attttctatc     1980

ttcaaaaatc ataaaattat gtttcttggc aagctccttc aaaggcaaca cagttccttc     2040

atacgtaaaa tcagaaacaa tatcgatgtg tgttttaact agacagatgt aaggaccaat     2100

agtgtccaaa atagagagaa gcttttcagt ttcagtaata tccaatgatg cacaaaggtt     2160

agacttcttt tcctccatga tggagaaaag tctcctagca acaggggaag tgtgtgattc     2220

tgatctttct ttgtatgacg ccatccttga caaacaaact actttattaa agcgttgaag     2280

atctattctc cagcaattaa atttgtgaag aataactggt atagagtact tcctttaaaa     2340

acatgtccgt gcaccaagaa aaaaaaaaag tttgaaaaat tgtatgtcga cgaatttcag     2400

cattttcatt tcaaggcgat attatgtttc actaaactca ggacaggaat atactaagaa     2460

taactacaac atacacacaa cataagccaa gatggatcaa cttaactacc aagaacaaca     2520

acaatttcaa aagatcgttg aacaaaagca aatggctgat ttcatgaggc tatgaattct     2580

tttattataa aattatatat tattcttaat tacatatcac ccttctatca gggaagggag     2640

aaacgaaaat agagagtgac ctatccaagc ttgggggtct aagttttaat ggcccaggga     2700

atcattactt ttttttctca atccttgatg gataaaagta ttacatacgt acaggattgt     2760

gtattagtgt atttcgttat atgattaaac aaagtttata gattgtaaag tagacgtaaa     2820

gtttagtaat tcattttaat gttcatttta cattcagatg ttaattaatt agaacaatct     2880

gttcaaacca tcgtaagctg caactctata tgcttcagcc attgttgggt agttgaaggt     2940

ggtattgacg aagtacttaa gagtattttg ttcacctggt tggttcataa ttgcctggcc     3000

aatatgaaca atttcggatg cttgataacc aaaacagtga acacctaaga cttctaaagt     3060

ttctctatgg aataagattt taagcatacc ctgtggttca ccagcaattt gtgctcttgc     3120

catagacttg aaaaaggctt taccgacctc ataaggaacc ttagctttag tcaattcgtg     3180

ttcattttta ccaattgagg aaatttctgg aatagtgtag ataccggttg gaacatcgtt     3240

gacatatctc caggaaccat tatcaacgat agagccagct gctgatctac cttggtcgtg     3300

agctgctgag gcaagtgatg gccaaccaat aacatcacca gcaccataaa tgtttgtgac     3360

acaggttctg taattctcgt caacttcaat ttgacctctg gagttgacct taacaccaat     3420

gttttccata cctaacttgt cagtattacc agttctaccg ttacaccata acaatgcatc     3480

ggccttaatc tttttaccgg acttcaaatg caaaataaca ccgttatcta aaccttcgac     3540

tctgtcgtat tcctcattat gtctaactgt aatgttgtta ttagaaaagt gataggaaag     3600

agcttgagaa atttcagagt ctaaaaagga caaaagttga tctctgttat cgaccaactc     3660

gactaagaca cccaaacctg aaaagataga agcatactcg caaccgataa caccggcacc     3720

gtagataatc aactttctag gggtgtggcc taaagataag atggtatcag agtcgtagat     3780

acgtggatga tggaaatcga tgtctgctgg tctgtaaggt ctggaacctg tggcgatgat     3840

aatatgctta gcaactaact tctcgacaac accatttgca caaacgactt caacagtctg     3900

ttcatctgcg aaggaaccag taccgaaaaa caaatcaact ctgtttctag cgtaatagcc     3960

ggtacgagaa gcgacttgct tggaaataac cttttctgca gactttaaaa catctggaaa     4020

ggagaaccat cttggttcgc caatggctct gaacattgga ttagtgttga attgcatgat     4080

ttgacgaaca gagtgtctca aagccttaga aggaatagta cctaagtggg tacagttacc     4140

accgacttgt ctacgtgaat caaccatagc aacttttctg cctgctttag ctgcattcat     4200

agcagcacct tcgcctgctg gaccagaacc caaaacaaca acgtcgtagt tataaacagc     4260

cattttatct agagaggggg ttatatgtgt aaatatagag tttgtgtttg gttgtgtatt     4320

gatggacact ggggaaagtt atctctctct tctaaagttc caacttcttc ctctctctgg     4380

gggtggacat ctcctgtctt tatataccac ccccccccct catagtctgg aactcttccc     4440

cgtccgagag cgacgtgatt ttccctatat ttctccttcc aaattgcccc attgaggcaa     4500

ccgcgtgata cgccgggtct tttcttcccg ggagtggaga cagttcccaa gtggtaaagt     4560

ccacttccat catgatccac ctccatgaca ctcttttaaa cacatgtgct gcacatgtgt     4620

tggtgtggtt acacgatata caaccatgtt gggcaaccac aggtgcgcag catcaacaac     4680

ggggcgagtc aggaggaacg gtaaccttgg cggtttacga atgaggtgtt ccccgttgga     4740

gaggtgtcta tggacgacca tcccagtaca atggacgggt tggttggtgt ggttacggtg     4800

tcaacgacag agacatgggc caacagagac attatggaga gacgttggga gtggatcctc     4860

cctggaaagg gtgtctttgg cgaagtgttg ttgatttgta tgtctgggtc ggttgtttgt     4920

tgtgctagtt gctgcataat ggtaccaaca ccagcggttt ttttgtcctc ctctgcaaca     4980

ggggaactcg gccacttgtt tattgggggg gggggggggg atgccacgtg gtaatgaaac     5040

attatcaaga gacctctctt tgtaggggta cctgtgatgt gtgtgtgtgt gcatgtgcat     5100

agatgcatat tgagacagtt gtgttcattg actgtctgca tttctccaga accaataaaa     5160

aataaaaata gtagcaatac ctacactttc tggaccttgt agtaacgtag acgccgtaag     5220

gttgcatttc acgccagctt gagaggggga aagaactacc aatctcgagg gatccgcggc     5280

cgcaaataaa tttaaaataa acgatatcaa aattcaaagg gttcaaagtg ggaattcctt     5340

gatttatata cacctttgcc aaccgcttgt tacttgataa ggaaaagata gatttctaaa     5400

gtgcaggaaa agaaacgcca ctacgtcatg aaacaaaaga aatgaaacac tctgcaaaag     5460

ggaaaaccaa tgacgccttc aaaacgtact gactttccgc ctccttttct gccttttttt     5520

tttctccctc aatttgccaa ttcccctttc cgctaatttt acatcacctt tttgtttgtt     5580

tcccttttcg gccaagtttt ccatttcttt tttcggctga gcccttcttt ggcgtcgacg     5640

taatttctcg gcatgtggcc aatgtatatt gacagtagat gaagtagacg ttcttagtaa     5700

ctgttagggt gagattgcca cccccccttc cttcttttac tatctgtaat accatcacca     5760

tagcaatagt ttaaccatgt tggagctgga aatacaacgt ctatagaggg aagtcatcat     5820

attacgccat tttacggacc agggacaccc tgtagtgtgt ttcctctctt gtagaggtag     5880

gttttcaaat ggactctggc gtcgatttcc agcaagtcat tcccgtggtt caccatttct     5940

actttttgcg ctacctctct tgacacagaa atgaatgatg acgtgtaaat tacccgtccg     6000

agacctggac tccggagaaa ctgtattaat tacgcgccaa acaagacagg tgtcggataa     6060

acgtgcatgt acagactgcg agccgaaaac ggaagggggg aaagaaaaca gtggagtccc     6120

attgttgttc cggaaatgga aaacgggaac tggcggaaaa gaaacgaaac aaaacaaaag     6180

aaaaagagga aaaaaaagaa aaaaaaaaga aaaagacact gcacgtgatt gctggtgtgt     6240

gctgcgtaac cgcggcactt tatttcgtaa atgaaggggc c                         6281


<210>  141
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  141
aaagggttaa ttaattagaa caatctgttc aaac                                   34


<210>  142
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  142
ctgttcaaac catcgtaagc                                                   20


<210>  143
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  143
gtagttgaag gtggtattaa cgaaatattc                                        30


<210>  144
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  144
ggaactgtgt tgcctttg                                                     18


<210>  145
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  145
gaataaaact ggtaggccta ttttctattc tc                                     32


<210>  146
<211>  464
<212>  PRT
<213>  Pseudomonas fluorescens

<400>  146

Met Ala Val Tyr Asn Tyr Asp Val Val Val Leu Gly Ser Gly Pro Ala 
1               5                   10                  15      


Gly Glu Gly Ala Ala Met Asn Ala Ala Lys Ala Gly Arg Lys Val Ala 
            20                  25                  30          


Met Val Asp Ser Arg Arg Gln Val Gly Gly Asn Cys Thr His Leu Gly 
        35                  40                  45              


Thr Ile Pro Ser Lys Ala Leu Arg His Ser Val Arg Gln Ile Met Gln 
    50                  55                  60                  


Phe Asn Thr Asn Pro Met Phe Arg Ala Ile Gly Glu Pro Arg Trp Phe 
65                  70                  75                  80  


Ser Phe Pro Asp Val Leu Lys Ser Ala Glu Lys Val Ile Ser Lys Gln 
                85                  90                  95      


Val Ala Ser Arg Thr Gly Tyr Tyr Ala Arg Asn Arg Val Asp Leu Phe 
            100                 105                 110         


Phe Gly Thr Gly Ser Phe Ala Asp Glu Gln Thr Val Glu Val Val Cys 
        115                 120                 125             


Ala Asn Gly Val Val Glu Lys Leu Val Ala Lys His Ile Ile Ile Ala 
    130                 135                 140                 


Thr Gly Ser Arg Pro Tyr Arg Pro Ala Asp Ile Asp Phe His His Pro 
145                 150                 155                 160 


Arg Ile Tyr Asp Ser Asp Thr Ile Leu Ser Leu Gly His Thr Pro Arg 
                165                 170                 175     


Lys Leu Ile Ile Tyr Gly Ala Gly Val Ile Gly Cys Glu Tyr Ala Ser 
            180                 185                 190         


Ile Phe Ser Gly Leu Gly Val Leu Val Glu Leu Val Asp Asn Arg Asp 
        195                 200                 205             


Gln Leu Leu Ser Phe Leu Asp Ser Glu Ile Ser Gln Ala Leu Ser Tyr 
    210                 215                 220                 


His Phe Ser Asn Asn Asn Ile Thr Val Arg His Asn Glu Glu Tyr Asp 
225                 230                 235                 240 


Arg Val Glu Gly Leu Asp Asn Gly Val Ile Leu His Leu Lys Ser Gly 
                245                 250                 255     


Lys Lys Ile Lys Ala Asp Ala Leu Leu Trp Cys Asn Gly Arg Thr Gly 
            260                 265                 270         


Asn Thr Asp Lys Leu Gly Met Glu Asn Ile Gly Val Lys Val Asn Ser 
        275                 280                 285             


Arg Gly Gln Ile Glu Val Asp Glu Asn Tyr Arg Thr Cys Val Thr Asn 
    290                 295                 300                 


Ile Tyr Gly Ala Gly Asp Val Ile Gly Trp Pro Ser Leu Ala Ser Ala 
305                 310                 315                 320 


Ala His Asp Gln Gly Arg Ser Ala Ala Gly Ser Ile Val Asp Asn Gly 
                325                 330                 335     


Ser Trp Arg Tyr Val Asn Asp Val Pro Thr Gly Ile Tyr Thr Ile Pro 
            340                 345                 350         


Glu Ile Ser Ser Ile Gly Lys Asn Glu His Glu Leu Thr Lys Ala Lys 
        355                 360                 365             


Val Pro Tyr Glu Val Gly Lys Ala Phe Phe Lys Ser Met Ala Arg Ala 
    370                 375                 380                 


Gln Ile Ala Gly Glu Pro Gln Gly Met Leu Lys Ile Leu Phe His Arg 
385                 390                 395                 400 


Glu Thr Leu Glu Val Leu Gly Val His Cys Phe Gly Tyr Gln Ala Ser 
                405                 410                 415     


Glu Ile Val His Ile Gly Gln Ala Ile Met Asn Gln Pro Gly Glu Gln 
            420                 425                 430         


Asn Thr Leu Lys Tyr Phe Val Asn Thr Thr Phe Asn Tyr Pro Thr Met 
        435                 440                 445             


Ala Glu Ala Tyr Arg Val Ala Ala Tyr Asp Gly Leu Asn Arg Leu Phe 
    450                 455                 460                 


