                                SEQUENCE LISTING

<110> Zahn, Kenneth
      Jacobson, Sandra
      EdeniQ, Inc.

<120> Improved Acetate Resistance in Yeast
  Based on Introduction of a Mutant HAA1 Allele
  

<130> 91997-881141

<140> WO Not yet assigned 
<141> Not yet assigned  

<150> US 61/674,676       
<151> 2012-07-23  

<160> 21

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 694
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> Saccharomyces cerevisiae strain S288c wildtype
      promoter DNA-binding transcription factor Haa1p,
      locus YPR008W, control Haa1 protein,
      transcriptional activator

<400> 1
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Ala Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Ile Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Gly Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Gln Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 2
<211> 694
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> Saccharomyces cerevisiae strain TR3 wildtype
      promoter DNA-binding transcription factor Haa1p,
      control Haa1 protein, transcriptional activator

<400> 2
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 3
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant mut2
      (TR3mut2), transcriptional activator

<400> 3
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Tyr Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Ser Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Val His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile Tyr Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Phe Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Phe Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Leu Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 4
<211> 554
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant mut40
      (TR3mut40), transcriptional activator

<400> 4
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Tyr Val Lys His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Val Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Ile
545                 550                 


<210> 5
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 440 F->Y,
      transcriptional activator

<400> 5
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Tyr Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 6
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 508 D->Y,
      transcriptional activator

<400> 6
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Tyr Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 7
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 510 N->K,
      transcriptional activator

<400> 7
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Lys His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 8
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 518 P->S,
      transcriptional activator

<400> 8
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Ser Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 9
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 527 A->V,
      transcriptional activator

<400> 9
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Val Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 10
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 554 N->I,
      transcriptional activator

<400> 10
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Ile Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 11
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 591 I->V,
      transcriptional activator

<400> 11
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Val His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 12
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 605 H->Y,
      transcriptional activator

<400> 12
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile Tyr Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 13
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 622 S->F,
      transcriptional activator

<400> 13
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Phe Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 14
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 639 S->F,
      transcriptional activator

<400> 14
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Phe Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 15
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant 673 S->L,
      transcriptional activator

<400> 15
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Leu Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 16
<211> 9
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter region sequence Haa1p
      transcription factor binding site

<220> 
<221> modified_base  
<222> (2)...(3)
<223> n = g, a, c or t

<400> 16
gnnsmrgvg                                                         9

<210> 17
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant mut24,
      transcriptional activator

<400> 17
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Ala Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Ile Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asn Gln Gly Phe Ala Asp Trp Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 18
<211> 616
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant mut33,
      transcriptional activator

<400> 18
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Val Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Met Tyr Gln Thr Arg
    610                 615     


<210> 19
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant mut36,
      transcriptional activator

<400> 19
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Asp Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Ser Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Phe Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Val Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 20
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      site-directed acetate resistant mutant mut5,
      transcriptional activator

<400> 20
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Leu Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Gly Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Asn Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


<210> 21
<211> 694
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Saccharomyces cerevisiae strain TR3
      promoter DNA-binding transcription factor Haa1p
      consensus sequence, transcriptional activator

<400> 21
Met Val Leu Ile Asn Gly Ile Lys Tyr Ala Cys Glu Arg Cys Ile Arg
 1               5                  10                  15      
Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Met Met
            20                  25                  30          
Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Asp Tyr Cys Lys Gln
        35                  40                  45              
Leu Arg Lys Asn Lys Asn Ala Asn Pro Glu Gly Val Cys Thr Cys Gly
    50                  55                  60                  
Arg Leu Glu Lys Lys Lys Leu Ala Gln Lys Ala Lys Glu Glu Ala Arg
65                  70                  75                  80  
Val Lys Ala Lys Glu Lys Gln Arg Lys Gln Cys Thr Cys Gly Thr Asp
                85                  90                  95      
Glu Val Cys Lys Tyr His Ala Gln Lys Arg His Leu Arg Lys Ser Pro
            100                 105                 110         
Ser Ser Ser Gln Lys Lys Gly Arg Ser Ile Ser Arg Ser Gln Pro Met
        115                 120                 125             
Phe Glu Arg Val Leu Ser Ser Thr Ser Leu Asp Ser Asn Met Leu Ser
    130                 135                 140                 
Gly His Gly Ala Leu Ser Asp Thr Ser Ser Ile Leu Thr Ser Thr Phe
145                 150                 155                 160 
Leu Asp Ser Glu Pro Gly Val Gly Lys Ile Ser Lys Asp Tyr His His
                165                 170                 175     
Val Pro Ser Leu Ala Ser Ile Ser Ser Leu Gln Ser Ser Gln Ser Leu
            180                 185                 190         
Asp Gln Asn Phe Ser Val Pro Gln Ser Pro Pro Leu Ser Ser Met Ser
        195                 200                 205             
Phe Asn Phe Leu Thr Gly Asn Ile Asn Glu Thr Asn Gln Asn His Ser
    210                 215                 220                 
Asn His Gln His Ser Lys Ser Ser Asn Asn Trp Gln Asp Ser Ser Val
225                 230                 235                 240 
Ser Leu Pro Ala Lys Ala Asp Ser Arg Leu Asn Met Met Asp Lys Asn
                245                 250                 255     
Asn Ser Val Gly Leu Asp Leu Leu Gly His Ser Lys Arg Ile Ser Pro
            260                 265                 270         
Ile Ser Asn Ser Arg Val Gly Glu Val Ser Val Pro Leu Glu Glu Tyr
        275                 280                 285             
Ile Pro Ser Asp Ile Asp Gly Val Gly Arg Val Thr Asp Lys Ser Ser
    290                 295                 300                 
Leu Val Tyr Asp Trp Pro Phe Asp Glu Ser Ile Glu Arg Asn Phe Ser
305                 310                 315                 320 
Thr Thr Ala Thr Ala Ala Thr Gly Glu Ser Lys Phe Asp Ile Asn Asp
                325                 330                 335     
Asn Cys Asn Arg Ile Asn Ser Lys Ser Tyr Ser Lys Thr Asn Ser Met
            340                 345                 350         
Asn Gly Asn Gly Met Asn Asn Ser Asn Asn Asn Asn Ile Asn Ser Asn
        355                 360                 365             
Gly Asn Asp Lys Asn Asn Asn Asn Ser Ser Arg Gln Glu His Gln Gly
    370                 375                 380                 
Asn Gly Leu Phe Asp Met Phe Thr Asp Ser Ser Ser Ile Ser Thr Leu
385                 390                 395                 400 
Ser Arg Ala Asn Leu Leu Leu Gln Glu Lys Ile Gly Ser Gln Glu Asn
                405                 410                 415     
Ser Val Lys Pro Glu Asn Tyr Ser Lys Asn Pro Gln Leu Arg His Gln
            420                 425                 430         
Leu Thr Ser Arg Ser Arg Ser Phe Ile His His Pro Ala Asn Glu Tyr
        435                 440                 445             
Leu Lys Asn Thr Phe Gly Asn Ser His Ser Asn Asp Ile Gly Lys Gly
    450                 455                 460                 
Val Glu Val Leu Ser Leu Thr Pro Ser Phe Met Asp Ile Pro Glu Lys
465                 470                 475                 480 
Glu Arg Glu Thr Glu Arg Ser Pro Ser Ser Asn Tyr Ile Thr Asp Arg
                485                 490                 495     
Pro Phe Thr Arg Lys Pro Arg Ser Ser Ser Ile Asp Val Asn His Arg
            500                 505                 510         
Tyr Pro Pro Met Ala Pro Thr Thr Val Ala Thr Ser Pro Gly Ala Leu
        515                 520                 525             
Asn Asn Ala Val Ala Ser Asn Leu Asp Asp Gln Leu Ser Leu Thr Ser
    530                 535                 540                 
Leu Asn Ser Gln Pro Ser Ser Ile Ala Asn Met Met Met Asp Pro Ser
545                 550                 555                 560 
Asn Leu Ala Glu Gln Ser Ser Ile His Ser Val Pro Gln Ser Ile Asn
                565                 570                 575     
Ser Pro Arg Met Pro Lys Thr Gly Ser Arg Gln Asp Lys Asn Ile His
            580                 585                 590         
Thr Lys Lys Glu Glu Arg Asn Pro Leu Asn Asn Ile His Asp Leu Ser
        595                 600                 605             
Gln Leu Glu Asn Val Pro Asp Glu Met Asn Gln Met Phe Ser Pro Pro
    610                 615                 620                 
Leu Lys Ser Met Asn Arg Pro Asp Ala Ile Arg Glu Asn Ser Ser Ser
625                 630                 635                 640 
Ser Asn Phe Ile Ile Gln Gly Asn Ser Met Ile Ser Thr Pro Ser Gly
                645                 650                 655     
Arg Asn Asp Leu Pro Asp Thr Ser Pro Met Ser Ser Ile Gln Thr Ala
            660                 665                 670         
Ser Pro Pro Ser Gln Leu Leu Thr Asp Gln Gly Phe Ala Asp Leu Asp
        675                 680                 685             
Asn Phe Met Ser Ser Leu
    690                 


