                         SEQUENCE LISTING

<110>  Merck Sharp & Dohme Corp.
       McNeely, Tessie B.
       Cope, Leslie
       Pancari, Gregory D.
       Joshi, Amita
 
<120>  PROTECTIVE VACCINE BASED ON STAPHYLOCOCCUS AUREUS SA2493 PROTEIN

<130>  MRL-IFD-00050

<150>  61/669,947
<151>  2012-07-10

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  795
<212>  DNA
<213>  Species: S. aureus

<400>  1
atgattcatt caaagaaatt aacgctcggt atatgcttgg tattactcat tatattgatt       60

gtaggttatg tcattatgac aaaaacaaat ggtcgaaacg cccaaattaa agacacattt      120

aatcaaacat taaaattata tccaaccaaa aatctcgatg atttttacga taaagaaggc      180

tttcgagatc aagaatttaa aaagggtgat aaaggtactt ggatagttaa ttctgaaatg      240

gtaatcgagc caaaaggtaa ggatatggaa acgagaggaa tggtgctcta tatcaatcgc      300

aatactagaa ccacaaaagg gtattatttt ataagtgaaa tgacagatga cagtaacggc      360

agaccaaagg atgatgaaaa aaggtatccg gtaaaaatgg aacataataa aatcatacca      420

acgaagccac taccgaatga caagttaaaa aaagagattg aaaactttaa gttctttgta      480

caatatggca actttaaaga tattaatgat tataaagatg gtgatatttc atataatcct      540

aatgtaccaa gttattcggc aaaatatcaa ttgaataatg atgattataa tgtccaacag      600

ttaagaaaaa gatatgatat tccaaccaaa caagcgccga aactattatt gaaaggcgat      660

ggagatttaa aaggttcatc cgtaggttct agaagtcttg aatttacctt tgtcgaaaat      720

aaagaagaaa acatttattt tacagattct gtacaatata ctccaagtga ggatacaagg      780

tatgagtcaa actga                                                       795


<210>  2
<211>  265
<212>  PRT
<213>  Species: S. aureus

<400>  2

Met Ile His Ser Lys Lys Leu Thr Leu Gly Ile Cys Leu Val Leu Leu 
1               5                   10                  15      


Ile Ile Leu Ile Val Gly Tyr Val Ile Met Thr Met Lys Thr Asn Gly 
            20                  25                  30          


Arg Asn Ala Gln Ile Lys Asp Thr Phe Asn Gln Thr Leu Lys Leu Tyr 
        35                  40                  45              


Pro Thr Lys Asn Leu Asp Asp Phe Tyr Asp Lys Glu Gly Phe Arg Asp 
    50                  55                  60                  


Gln Glu Phe Lys Lys Gly Asp Lys Gly Thr Trp Ile Val Asn Ser Glu 
65                  70                  75                  80  


Met Val Ile Glu Pro Lys Gly Lys Asp Met Glu Thr Arg Gly Met Val 
                85                  90                  95      


Leu Tyr Ile Asn Arg Asn Thr Arg Thr Thr Lys Gly Tyr Tyr Phe Ile 
            100                 105                 110         


Ser Glu Met Thr Asp Asp Ser Asn Gly Arg Pro Lys Asp Asp Glu Lys 
        115                 120                 125             


Arg Tyr Pro Val Lys Met Glu His Asn Lys Ile Ile Pro Thr Lys Pro 
    130                 135                 140                 


Leu Pro Asn Asp Lys Leu Lys Lys Glu Ile Glu Asn Phe Lys Phe Phe 
145                 150                 155                 160 


Val Gln Tyr Gly Asn Phe Lys Asp Ile Asn Asp Tyr Lys Asp Gly Asp 
                165                 170                 175     


Ile Ser Tyr Asn Pro Asn Val Pro Ser Tyr Ser Ala Lys Tyr Gln Leu 
            180                 185                 190         


Asn Asn Asp Asp Tyr Asn Val Gln Gln Leu Arg Lys Arg Tyr Asp Ile 
        195                 200                 205             


Pro Thr Lys Gln Ala Pro Lys Leu Leu Leu Lys Gly Asp Gly Asp Leu 
    210                 215                 220                 


Lys Gly Ser Ser Val Gly Ser Arg Ser Leu Glu Phe Thr Phe Val Glu 
225                 230                 235                 240 


Asn Lys Glu Glu Asn Ile Tyr Phe Thr Asp Ser Val Gln Tyr Thr Pro 
                245                 250                 255     


Ser Glu Asp Thr Arg Tyr Glu Ser Asn 
            260                 265 


<210>  3
<211>  819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SA2493 with His-tag

<400>  3
atgattcatt caaagaaatt aacgctcggt atatgcttgg tattactcat tatattgatt       60

gtaggttatg tcattatgac aaaaacaaat ggtcgaaacg cccaaattaa agacacattt      120

aatcaaacat taaaattata tccaaccaaa aatctcgatg atttttacga taaagaaggc      180

tttcgagatc aagaatttaa aaagggtgat aaaggtactt ggatagttaa ttctgaaatg      240

gtaatcgagc caaaaggtaa ggatatggaa acgagaggaa tggtgctcta tatcaatcgc      300

aatactagaa ccacaaaagg gtattatttt ataagtgaaa tgacagatga cagtaacggc      360

agaccaaagg atgatgaaaa aaggtatccg gtaaaaatgg aacataataa aatcatacca      420

acgaagccac taccgaatga caagttaaaa aaagagattg aaaactttaa gttctttgta      480

caatatggca actttaaaga tattaatgat tataaagatg gtgatatttc atataatcct      540

aatgtaccaa gttattcggc aaaatatcaa ttgaataatg atgattataa tgtccaacag      600

ttaagaaaaa gatatgatat tccaaccaaa caagcgccga aactattatt gaaaggcgat      660

ggagatttaa aaggttcatc cgtaggttct agaagtcttg aatttacctt tgtcgaaaat      720

aaagaagaaa acatttattt tacagattct gtacaatata ctccaagtga ggatacaagg      780

tatgagtcaa accaccacca ccaccaccac caccactga                             819


<210>  4
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SA2493 with His-tag

<400>  4

Met Ile His Ser Lys Lys Leu Thr Leu Gly Ile Cys Leu Val Leu Leu 
1               5                   10                  15      


Ile Ile Leu Ile Val Gly Tyr Val Ile Met Thr Lys Thr Asn Gly Arg 
            20                  25                  30          


Asn Ala Gln Ile Lys Asp Thr Phe Asn Gln Thr Leu Lys Leu Tyr Pro 
        35                  40                  45              


Thr Lys Asn Leu Asp Asp Phe Tyr Asp Lys Glu Gly Phe Arg Asp Gln 
    50                  55                  60                  


Glu Phe Lys Lys Gly Asp Lys Gly Thr Trp Ile Val Asn Ser Glu Met 
65                  70                  75                  80  


Val Ile Glu Pro Lys Gly Lys Asp Met Glu Thr Arg Gly Met Val Leu 
                85                  90                  95      


Tyr Ile Asn Arg Asn Thr Arg Thr Thr Lys Gly Tyr Tyr Phe Ile Ser 
            100                 105                 110         


Glu Met Thr Asp Asp Ser Asn Gly Arg Pro Lys Asp Asp Glu Lys Arg 
        115                 120                 125             


Tyr Pro Val Lys Met Glu His Asn Lys Ile Ile Pro Thr Lys Pro Leu 
    130                 135                 140                 


Pro Asn Asp Lys Leu Lys Lys Glu Ile Glu Asn Phe Lys Phe Phe Val 
145                 150                 155                 160 


Gln Tyr Gly Asn Phe Lys Asp Ile Asn Asp Tyr Lys Asp Gly Asp Ile 
                165                 170                 175     


Ser Tyr Asn Pro Asn Val Pro Ser Tyr Ser Ala Lys Tyr Gln Leu Asn 
            180                 185                 190         


Asn Asp Asp Tyr Asn Val Gln Gln Leu Arg Lys Arg Tyr Asp Ile Pro 
        195                 200                 205             


Thr Lys Gln Ala Pro Lys Leu Leu Leu Lys Gly Asp Gly Asp Leu Lys 
    210                 215                 220                 


Gly Ser Ser Val Gly Ser Arg Ser Leu Glu Phe Thr Phe Val Glu Asn 
225                 230                 235                 240 


Lys Glu Glu Asn Ile Tyr Phe Thr Asp Ser Val Gln Tyr Thr Pro Ser 
                245                 250                 255     


Glu Asp Thr Arg Tyr Glu Ser Asn His His His His His His His His 
            260                 265                 270         


<210>  5
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  5
caccatgatt cattcaaaga aattaacgct cggtatatgc ttggt                       45


<210>  6
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  6
tcagtggtgg tggtggtggt ggtggtggtt tgactcatac cttgt                       45


<210>  7
<211>  645
<212>  PRT
<213>  Species: S. aureus

<400>  7

Met Asn Lys Gln Gln Lys Glu Phe Lys Ser Phe Tyr Ser Ile Arg Lys 
1               5                   10                  15      


Ser Ser Leu Gly Val Ala Ser Val Ala Ile Ser Thr Leu Leu Leu Leu 
            20                  25                  30          


Met Ser Asn Gly Glu Ala Gln Ala Ala Ala Glu Glu Thr Gly Gly Thr 
        35                  40                  45              


Asn Thr Glu Ala Gln Pro Lys Thr Glu Ala Val Ala Ser Pro Thr Thr 
    50                  55                  60                  


Thr Ser Glu Lys Ala Pro Glu Thr Lys Pro Val Ala Asn Ala Val Ser 
65                  70                  75                  80  


Val Ser Asn Lys Glu Val Glu Ala Pro Thr Ser Glu Thr Lys Glu Ala 
                85                  90                  95      


Lys Glu Val Lys Glu Val Lys Ala Pro Lys Glu Thr Lys Glu Val Lys 
            100                 105                 110         


Pro Ala Ala Lys Ala Thr Asn Asn Thr Tyr Pro Ile Leu Asn Gln Glu 
        115                 120                 125             


Leu Arg Glu Ala Ile Lys Asn Pro Ala Ile Lys Asp Lys Asp His Ser 
    130                 135                 140                 


Ala Pro Asn Ser Arg Pro Ile Asp Phe Glu Met Lys Lys Lys Asp Gly 
145                 150                 155                 160 


Thr Gln Gln Phe Tyr His Tyr Ala Ser Ser Val Lys Pro Ala Arg Val 
                165                 170                 175     


Ile Phe Thr Asp Ser Lys Pro Glu Ile Glu Leu Gly Leu Gln Ser Gly 
            180                 185                 190         


Gln Phe Trp Arg Lys Phe Glu Val Tyr Glu Gly Asp Lys Lys Leu Pro 
        195                 200                 205             


Ile Lys Leu Val Ser Tyr Asp Thr Val Lys Asp Tyr Ala Tyr Ile Arg 
    210                 215                 220                 


Phe Ser Val Ser Asn Gly Thr Lys Ala Val Lys Ile Val Ser Ser Thr 
225                 230                 235                 240 


His Phe Asn Asn Lys Glu Glu Lys Tyr Asp Tyr Thr Leu Met Glu Phe 
                245                 250                 255     


Ala Gln Pro Ile Tyr Asn Ser Ala Asp Lys Phe Lys Thr Glu Glu Asp 
            260                 265                 270         


Tyr Lys Ala Glu Lys Leu Leu Ala Pro Tyr Lys Lys Ala Lys Thr Leu 
        275                 280                 285             


Glu Arg Gln Val Tyr Glu Leu Asn Lys Ile Gln Asp Lys Leu Pro Glu 
    290                 295                 300                 


Lys Leu Lys Ala Glu Tyr Lys Lys Lys Leu Glu Asp Thr Lys Lys Ala 
305                 310                 315                 320 


Leu Asp Glu Gln Val Lys Ser Ala Ile Thr Glu Phe Gln Asn Val Gln 
                325                 330                 335     


Pro Thr Asn Glu Lys Met Thr Asp Leu Gln Asp Thr Lys Tyr Val Val 
            340                 345                 350         


Tyr Glu Ser Val Glu Asn Asn Glu Ser Met Met Asp Thr Phe Val Lys 
        355                 360                 365             


His Pro Ile Lys Thr Gly Met Leu Asn Gly Lys Lys Tyr Met Val Met 
    370                 375                 380                 


Glu Thr Thr Asn Asp Asp Tyr Trp Lys Asp Phe Met Val Glu Gly Gln 
385                 390                 395                 400 


Arg Val Arg Thr Ile Ser Lys Asp Ala Lys Asn Asn Thr Arg Thr Ile 
                405                 410                 415     


Ile Phe Pro Tyr Val Glu Gly Lys Thr Leu Tyr Asp Ala Ile Val Lys 
            420                 425                 430         


Val His Val Lys Thr Ile Asp Tyr Asp Gly Gln Tyr His Val Arg Ile 
        435                 440                 445             


Val Asp Lys Glu Ala Phe Thr Lys Ala Asn Thr Asp Lys Ser Asn Lys 
    450                 455                 460                 


Lys Glu Gln Gln Asp Asn Ser Ala Lys Lys Glu Ala Thr Pro Ala Thr 
465                 470                 475                 480 


Pro Ser Lys Pro Thr Pro Ser Pro Val Glu Lys Glu Ser Gln Lys Gln 
                485                 490                 495     


Asp Ser Gln Lys Asp Asp Asn Lys Gln Leu Pro Ser Val Glu Lys Glu 
            500                 505                 510         


Asn Asp Ala Ser Ser Glu Ser Gly Lys Asp Lys Thr Pro Ala Thr Lys 
        515                 520                 525             


Pro Thr Lys Gly Glu Val Glu Ser Ser Ser Thr Thr Pro Thr Lys Val 
    530                 535                 540                 


Val Ser Thr Thr Gln Asn Val Ala Lys Pro Thr Thr Ala Ser Ser Lys 
545                 550                 555                 560 


Thr Thr Lys Asp Val Val Gln Thr Ser Ala Gly Ser Ser Glu Ala Lys 
                565                 570                 575     


Asp Ser Ala Pro Leu Gln Lys Ala Asn Ile Lys Asn Thr Asn Asp Gly 
            580                 585                 590         


His Thr Gln Ser Gln Asn Asn Lys Asn Thr Gln Glu Asn Lys Ala Lys 
        595                 600                 605             


Ser Leu Pro Gln Thr Gly Glu Glu Ser Asn Lys Asp Met Thr Leu Pro 
    610                 615                 620                 


Leu Met Ala Leu Leu Ala Leu Ser Ser Ile Val Ala Phe Val Leu Pro 
625                 630                 635                 640 


Arg Lys Arg Lys Asn 
                645 


