               SEQUENCE LISTING

<110> CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

<120> UTILIZACION DE SENALES DE LOCALIZACION NUCLEAR DE PROTEINAS DE BACTERIOFAGOS COMO VEHICULO PARA TRANSFERENCIA DE GENES

<130> 5120081 MARGARITA SALAS

<160> 29

<170> BiSSAP 1.0

<210> 1
<211> 266
<212> PRT
<213> Bacillus phage phi29

<220> 
<221> SOURCE
<222> 1..266
<223> /mol_type="protein"
      /note="Secuencia aminoacidica de la proteina terminal del bacteriofago phi29"
      /organism="Bacillus phage phi29"

<220> 
<221> DOMAIN
<222> 1..73
<223> dominio N terminal

<220> 
<221> SITE
<222> 1..37
<223> Secuencia de localizacion nuclear determinada experimentalmente

<220> 
<221> SITE
<222> 25..34
<223> Secuencia de localizacion nuclear predicha

<400> 1
Met Ala Arg Ser Pro Arg Ile Arg Ile Lys Asp Asn Asp Lys Ala Glu 
1               5                   10                   15    
Tyr Ala Arg Leu Val Lys Asn Thr Lys Ala Lys Ile Ala Arg Thr Lys 
            20                   25                  30        
Lys Lys Tyr Gly Val Asp Leu Thr Ala Glu Ile Asp Ile Pro Asp Leu 
        35                   40                  45            
Asp Ser Phe Glu Thr Arg Ala Gln Phe Asn Lys Trp Lys Glu Gln Ala 
    50                   55                  60                
Ser Ser Phe Thr Asn Arg Ala Asn Met Arg Tyr Gln Phe Glu Lys Asn 
65                   70                  75                  80
Ala Tyr Gly Val Val Ala Ser Lys Ala Lys Ile Ala Glu Ile Glu Arg 
                85                   90                  95    
Asn Thr Lys Glu Val Gln Arg Leu Val Asp Glu Lys Ile Lys Ala Met 
            100                  105                110        
Lys Asp Lys Glu Tyr Tyr Ala Gly Gly Lys Pro Gln Gly Thr Ile Glu 
        115                  120                125            
Gln Arg Ile Ala Met Thr Ser Pro Ala His Val Thr Gly Ile Asn Arg 
    130                  135                140                
Pro His Asp Phe Asp Phe Ser Lys Val Arg Ser Tyr Ser Arg Leu Arg 
145                  150                155                  160
Thr Leu Glu Glu Ser Met Glu Met Arg Thr Asp Pro Gln Tyr Tyr Glu 
                165                  170                175    
Lys Lys Met Ile Gln Leu Gln Leu Asn Phe Ile Lys Ser Val Glu Gly 
            180                  185                190        
Ser Phe Asn Ser Phe Asp Ala Ala Asp Glu Leu Ile Glu Glu Leu Lys 
        195                  200                205            
Lys Ile Pro Pro Asp Asp Phe Tyr Glu Leu Phe Leu Arg Ile Ser Glu 
    210                  215                220                
Ile Ser Phe Glu Glu Phe Asp Ser Glu Gly Asn Thr Val Glu Asn Val 
225                  230                235                  240
Glu Gly Asn Val Tyr Lys Ile Leu Ser Tyr Leu Glu Gln Tyr Arg Arg 
                245                  250                255    
Gly Asp Phe Asp Leu Ser Leu Lys Gly Phe 
            260                  265    

<210> 2
<211> 72
<212> PRT
<213> Bacillus phage phi29

<220> 
<221> SOURCE
<222> 1..72
<223> /mol_type="protein"
      /note="Secuencia aminoacidica del domino N terminal de la proteina terminal del bacteriofago phi29"
      /organism="Bacillus phage phi29"

<400> 2
Met Ala Arg Ser Pro Arg Ile Arg Ile Lys Asp Asn Asp Lys Ala Glu 
1               5                   10                   15    
Tyr Ala Arg Leu Val Lys Asn Thr Lys Ala Lys Ile Ala Arg Thr Lys 
            20                   25                  30        
Lys Lys Tyr Gly Val Asp Leu Thr Ala Glu Ile Asp Ile Pro Asp Leu 
        35                   40                  45            
Asp Ser Phe Glu Thr Arg Ala Gln Phe Asn Lys Trp Lys Glu Gln Ala 
    50                   55                  60                
Ser Ser Phe Thr Asn Arg Ala Asn 
65                   70        

<210> 3
<211> 37
<212> PRT
<213> Bacillus phage phi29

<220> 
<221> SOURCE
<222> 1..37
<223> /mol_type="protein"
      /note="Secuencia de localizacion nuclear de la proteina terminal del bacteriofago phi29 determinada experimentalmente"
      /organism="Bacillus phage phi29"

<400> 3
Met Ala Arg Ser Pro Arg Ile Arg Ile Lys Asp Asn Asp Lys Ala Glu 
1               5                   10                   15    
Tyr Ala Arg Leu Val Lys Asn Thr Lys Ala Lys Ile Ala Arg Thr Lys 
            20                   25                  30        
Lys Lys Tyr Gly Val 
        35        

<210> 4
<211> 266
<212> PRT
<213> Bacillus phage Nf

<220> 
<221> SOURCE
<222> 1..266
<223> /mol_type="protein"
      /note="Secuencia aminoacidica de la proteina terminal del bacteriofago Nf"
      /organism="Bacillus phage Nf"

<220> 
<221> SITE
<222> 25..34
<223> Secuencia de localizacion nuclear predicha

<400> 4
Met Ala Arg Asn Ser Arg Ile Arg Ile Thr Asn Asn Asp Lys Ala Leu 
1               5                   10                   15    
Tyr Ala Lys Leu Val Lys Asn Thr Lys Ala Lys Ile Ser Arg Thr Lys 
            20                   25                  30        
Lys Lys Tyr Gly Ile Asp Leu Ser Asn Glu Ile Glu Leu Pro Pro Leu 
        35                   40                  45            
Glu Ser Phe Gln Thr Arg Glu Glu Phe Asn Lys Trp Lys Gln Lys Gln 
    50                   55                  60                
Glu Ser Phe Thr Asn Arg Ala Asn Gln Asn Tyr Gln Phe Val Lys Asn 
65                   70                  75                  80
Lys Tyr Gly Ile Val Ala Ser Lys Ala Lys Ile Asn Glu Ile Ala Lys 
                85                   90                  95    
Asn Thr Lys Glu Ala Gln Arg Ile Val Asp Glu Gln Arg Glu Glu Ile 
            100                  105                110        
Glu Asp Lys Pro Phe Ile Ser Gly Gly Lys Gln Gln Gly Thr Val Gly 
        115                  120                125            
Gln Arg Met Gln Ile Leu Ser Pro Ser Gln Val Thr Gly Ile Ser Arg 
    130                  135                140                
Pro Ser Asp Phe Asn Phe Asp Asp Val Arg Ser Tyr Ala Arg Leu Arg 
145                  150                155                  160
Thr Leu Glu Glu Gly Met Ala Glu Lys Ala Ser Pro Asp Tyr Tyr Asp 
                165                  170                175    
Arg Arg Met Thr Gln Met His Gln Asn Phe Ile Glu Ile Val Glu Lys 
            180                  185                190        
Ser Phe Asn Ser Asp Trp Leu Ser Asp Glu Leu Val Glu Arg Leu Lys 
        195                  200                205            
Lys Ile Pro Pro Asp Asp Phe Phe Glu Leu Tyr Leu Met Phe Asp Glu 
    210                  215                220                
Ile Ser Phe Glu Tyr Phe Asp Ser Glu Gly Glu Asp Val Glu Ala Ser 
225                  230                235                  240
Glu Ala Met Leu Asn Lys Ile His Ser Tyr Leu Asp Arg Tyr Glu Arg 
                245                  250                255    
Gly Asp Val Asn Leu Asp Leu Lys Gly Phe 
            260                  265    

<210> 5
<211> 230
<212> PRT
<213> Streptococcus phage Cp1

<220> 
<221> SOURCE
<222> 1..230
<223> /mol_type="protein"
      /note="Secuencia aminoacidica de la proteina terminal del bacteriofago Cp1"
      /organism="Streptococcus phage Cp1"

<220> 
<221> SITE
<222> 222..230
<223> Secuencia de localizacion nuclear predicha

<220> 
<221> SITE
<222> 223..227
<223> Secuencia de localizacion nuclear predicha

<220> 
<221> SITE
<222> 5..11
<223> Secuencia de localizacion nuclear predicha

<220> 
<221> SITE
<222> 17..20
<223> Secuencia de localizacion nuclear predicha

<400> 5
Met Ala Leu Thr Pro Lys Gln Arg Lys Val Arg Arg Asp Tyr Leu Thr 
1               5                   10                   15    
Arg Lys Lys Arg Thr Leu Gln Gln Gln Gly Ala Ser Asn Ala Glu Ile 
            20                   25                  30        
Lys Ala Phe Met Gly Gly Arg Trp Asn Phe Ala Gly Met Ser Asp Lys 
        35                   40                  45            
Ala Leu Glu Arg Ala Tyr Asp Glu Ile Lys Ser Lys Gly Arg Thr Gln 
    50                   55                  60                
Val Phe Gly Asn His Val Tyr Thr Ser Asp Tyr Val Lys Lys Ala Lys 
65                   70                  75                  80
Ala Trp Tyr Gly Asp Lys Phe Ser Val Glu Lys Leu Thr Gln Gly Phe 
                85                   90                  95    
Arg Ser Ser Gln Arg Ser Glu Leu Asn Arg Phe His Ser Val Lys Glu 
            100                  105                110        
Val Lys Glu Tyr Arg Ser Glu Arg Asp Arg Glu Ala Lys Glu Arg Tyr 
        115                  120                125            
Ile Gln Ala Leu Glu Glu Met His Tyr Asn Thr Arg Asp Ala Gly Asn 
    130                  135                140                
Lys Ala Gln Glu Lys Ala Phe Lys Ser Met Ile Ser Arg Ile Arg Arg 
145                  150                155                  160
Met Ser Ala Ser Asn Phe Gly Ala Phe Leu Thr Gly Gly Arg Ser Asp 
                165                  170                175    
Lys Val Ser Phe Asp Asn Val Met Val Phe Val Asp Thr Asp Gly Lys 
            180                  185                190        
Glu Thr Ala Phe Glu Phe Gln Asp Ser Leu Ala Arg Glu Ile Leu Asp 
        195                  200                205            
Asn Val Asp Lys Phe Ser Lys Gln Phe Val Ser Asp Met Arg Arg Arg 
    210                  215                220                
Lys Lys Arg Gly Lys Lys 
225                  230

<210> 6
<211> 245
<212> PRT
<213> Bacillus phage Bam35c

<220> 
<221> SOURCE
<222> 1..245
<223> /mol_type="protein"
      /note="Secuencia aminoacidica de la proteina terminal del bacteriofago Bam35"
      /organism="Bacillus phage Bam35c"

<220> 
<221> SITE
<222> 4..55
<223> Secuencia de localizacion nuclear predicha

<220> 
<221> SITE
<222> 214..220
<223> Secuencia de localizacion nuclear predicha

<220> 
<221> SITE
<222> 14..31
<223> Secuencia de localizacion nuclear predicha

<400> 6
Met Ala Asn Lys Arg Leu Lys Lys Lys Leu Glu Thr Lys Arg Lys Lys 
1               5                   10                   15    
Ser Leu Leu Val Ser Glu Gly Tyr Ser Lys Lys Glu Thr Lys Lys Leu 
            20                   25                  30        
Lys Gly Arg Glu Leu Glu Thr Val Tyr Lys Lys Lys Ala His Asn Arg 
        35                   40                  45            
Lys Asn Arg Glu Arg Ala Arg Glu Ile Ala Asn Leu Ala Lys Gln Trp 
    50                   55                  60                
Gly Leu Ser Pro Ser Lys Tyr Asn Ser Trp Lys Lys Leu Leu Pro Glu 
65                   70                  75                  80
Ile Glu Arg Ile Lys Lys Glu Gln Asp Arg Glu Ala Pro Phe Leu Leu 
                85                   90                  95    
Ile Tyr Tyr Gln Asp Phe Thr Gly Glu Thr Asp Ser Lys Phe Ile Tyr 
            100                  105                110        
Asp Phe Lys Lys Arg Asn Asn Thr Arg Ser Arg Ser Gln Ile Thr Glu 
        115                  120                125            
Ser Ile Ile Gly Trp Leu Gln Asn Ala His Asn Lys Leu Phe Leu Gly 
    130                  135                140                
Arg Val Ala Ile Arg Ile Val Pro Lys Arg Asp Val Ser Lys Thr Asn 
145                  150                155                  160
Thr Leu Trp Arg Asn His Gly Tyr Val Lys Ile Tyr Glu Gly Gln Gly 
                165                  170                175    
Lys Glu Leu Ser Lys Leu Leu Thr Ala Ile Glu Thr Ile Met Val Gly 
            180                  185                190        
Val Tyr Asp Val Lys Glu Arg Asp Lys Tyr Leu Lys Glu Leu Val Ala 
        195                  200                205            
Lys Leu Arg Ser Leu Pro Tyr Glu Lys Ala Lys Lys Asn Ala Lys Glu 
    210                  215                220                
Ile Gln Lys Ile Tyr Asp Thr Lys Ser Tyr Lys Lys Glu Ser Trp Asp 
225                  230                235                  240
Asn Asp Asp Tyr Tyr 
                245

<210> 7
<211> 259
<212> PRT
<213> Enterobacteria phage PRD1

<220> 
<221> SOURCE
<222> 1..259
<223> /mol_type="protein"
      /note="Secuencia aminoacidica de la proteina terminal del bacteriofago PRD1"
      /organism="Enterobacteria phage PRD1"

<220> 
<221> SITE
<222> 25..41
<223> Secuencia de localizacion nuclear predicha

<220> 
<221> SITE
<222> 243..259
<223> Secuencia de localizacion nuclear predicha

<220> 
<221> SITE
<222> 3..6
<223> Secuencia de localizacion nuclear predicha

<400> 7
Met Ala Lys Lys Lys Pro Val Glu Lys Asn Gly Leu Val Tyr Lys Glu 
1               5                   10                   15    
Phe Gln Lys Gln Val Ser Asn Leu Lys Lys Ala Gly Leu Ile Pro Lys 
            20                   25                  30        
Thr Leu Asp Val Arg Lys Val Lys Pro Thr Lys His Tyr Lys Gly Leu 
        35                   40                  45            
Val Ser Lys Tyr Lys Asp Val Ala Thr Gly Gly Ala Lys Leu Ala Ala 
    50                   55                  60                
Ile Pro Asn Pro Ala Val Ile Glu Thr Leu Glu Ala Arg Gly Glu Ser 
65                   70                  75                  80
Ile Ile Lys Lys Gly Gly Lys Ala Tyr Leu Lys Ala Arg Gln Gln Ile 
                85                   90                  95    
Asn Gln Arg Gly Gln Ile Val Asn Pro Phe Thr Val Arg Val Thr Lys 
            100                  105                110        
Arg Gly Glu Val Val Arg Arg Tyr Arg Lys Thr Thr Pro Glu Gly Lys 
        115                  120                125            
Pro Val Tyr Ile Thr Gln Arg Glu Leu Pro Ile Lys Phe Glu Asn Met 
    130                  135                140                
Glu Gln Trp Leu Thr Glu Leu Lys Ala Ala Gly Phe Gln Leu Gln Pro 
145                  150                155                  160
Gly Glu Gln Ile Tyr Phe Thr Phe Asn Gly Asn Tyr Ser Arg Arg Thr 
                165                  170                175    
Tyr Thr Ser Phe Asp Glu Ala Phe Asn Lys Phe Met Thr Tyr Asp Ile 
            180                  185                190        
Ile Ile Asp Ala Val Ala Gly Lys Leu Lys Val Glu Asp Glu Ala Asp 
        195                  200                205            
Leu Val Lys Ser Val Gly Phe Gln Arg Ile Ser Gly Pro Glu Ala Lys 
    210                  215                220                
Ala Tyr Asn Arg Asn Arg Ile Val Leu Pro Glu Met Gln Phe Ser Gln 
225                  230                235                  240
Ala Ala Lys Lys Lys Tyr Lys Arg Arg Gln Lys Arg Gly Tyr Gly Ser 
                245                  250                255    
Lys Gly Val 
            

<210> 8
<211> 4731
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..4731
<223> /mol_type="DNA"
      /note="Plasmido de origen comercial Clontech"
      /organism="Artificial Sequence"

<400> 8
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc     1380

gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta     1440

gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg     1500

aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat     1560

agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc     1620

aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc     1680

gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc     1740

ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag     1800

tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga     1860

tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc     1920

actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa     1980

cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt     2040

agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc     2100

gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat     2160

acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg     2220

aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt     2280

ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca     2340

gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat     2400

ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg     2460

cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc     2520

gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta     2580

ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga     2640

tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc     2700

acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc     2760

ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc     2820

gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac     2880

tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc     2940

tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac     3000

gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg     3060

tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct     3120

cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt     3180

cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg     3240

attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac     3300

ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg     3360

tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg     3420

agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat     3480

ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc     3540

ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg     3600

aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata     3660

aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc     3720

cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt     3780

tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac     3840

gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta     3900

aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc     3960

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     4020

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     4080

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     4140

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     4200

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     4260

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     4320

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     4380

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     4440

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     4500

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     4560

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     4620

gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc     4680

tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t              4731


<210> 9
<211> 5513
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5513
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_TPphi29 procede del plasmido pEYFP_C1 modificado para que codifique por una proteina de fusion YFP_TPphi29"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 1360..2166
<223> /gene=Gen 3 del fago phi29 completo

<400> 9
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagct caagcttcga attctatggc gagaagtcca     1380

cgtatacgca ttaaggataa tgacaaagcc gaatacgctc gattggtcaa gaatacaaaa     1440

gccaagattg cgagaacgaa gaaaaagtat ggtgtagacc ttaccgctga aattgatata     1500

cctgaccttg attcatttga aacacgggcg cagttcaata agtggaagga acaagcgtcc     1560

tctttcacta accgtgctaa tatgcgttat cagttcgaaa agaatgcata cggtgtggtg     1620

gctagtaaag ctaagatagc tgagattgaa cgtaacacaa aagaggttca gcggttagta     1680

gatgagaaaa tcaaggctat gaaagacaaa gaatactatg caggcggtaa gccgcaaggg     1740

acaattgaac aacggatagc tatgacaagt cctgcacacg ttacaggaat taatagaccc     1800

catgattttg actttagcaa ggtgcgaagc tatagccgtt tgcgaaccct agaagaaagc     1860

atggagatga gaacagaccc tcagtattat gaaaagaaaa tgatacagtt acagttaaac     1920

tttattaaga gcgttgaggg tagtttcaat tcatttgatg cggcagatga actgatcgaa     1980

gaattaaaaa agatacctcc tgatgacttc tatgaattgt ttctcagaat atcagaaata     2040

tcctttgagg aatttgatag tgagggaaac acagtggaga acgtagaagg taatgtatat     2100

aaaatactgt catacttgga acagtatcga aggggtgact ttgatctaag cttaaagggg     2160

ttctaataac gggatccacc ggatctagat aactgatcat aatcagccat accacatttg     2220

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa     2280

tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca     2340

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt     2400

ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc     2460

gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc     2520

ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag     2580

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc     2640

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa     2700

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg     2760

aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt     2820

gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc     2880

gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa     2940

atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat     3000

tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt     3060

gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     3120

cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     3180

atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     3240

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     3300

ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     3360

taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     3420

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     3480

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     3540

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca     3600

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     3660

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     3720

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     3780

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     3840

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     3900

ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc     3960

gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     4020

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     4080

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     4140

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     4200

tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag     4260

atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg     4320

ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg     4380

ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa     4440

taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt     4500

cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg     4560

tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca     4620

acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt     4680

taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga     4740

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca     4800

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     4860

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     4920

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     4980

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     5040

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     5100

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     5160

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     5220

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     5280

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     5340

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     5400

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     5460

tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat            5513


<210> 10
<211> 4931
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..4931
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_NtTPphi29 procede del plasmido pEYFP_C1 modificado para que codifique por una proteina de fusion YPF_Nt"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 1360..1584
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente al fragmento N terminal de la proteina

<400> 10
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagct caagcttcga attctatggc gagaagtcca     1380

cgtatacgca ttaaggataa tgacaaagcc gaatacgctc gattggtcaa gaatacaaaa     1440

gccaagattg cgagaacgaa gaaaaagtat ggtgtagacc ttaccgctga aattgatata     1500

cctgaccttg attcatttga aacacgggcg cagttcaata agtggaagga acaagcgtcc     1560

tctttcacta accgtgctaa ttaataacgg gatccaccgg atctagataa ctgatcataa     1620

tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc     1680

tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata     1740

atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc     1800

attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt     1860

aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag     1920

gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt     1980

gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga     2040

aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg     2100

gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct     2160

tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc     2220

gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt     2280

aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct     2340

atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga     2400

taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga     2460

atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa     2520

gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca     2580

gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc     2640

ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt     2700

tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag     2760

gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg     2820

tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg     2880

ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg     2940

ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat     3000

gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca     3060

gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg     3120

gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat     3180

gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa     3240

catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg     3300

gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg     3360

cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg     3420

gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat     3480

caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac     3540

cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc     3600

cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc     3660

ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg     3720

gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt     3780

tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa     3840

cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc     3900

ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt     3960

ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag     4020

gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat     4080

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     4140

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     4200

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     4260

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     4320

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc     4380

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     4440

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     4500

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     4560

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     4620

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     4680

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     4740

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     4800

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct     4860

ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta     4920

ccgccatgca t                                                          4931


<210> 11
<211> 5234
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5234
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_NtITPphi29 procede del plasmido pEYFP_C1 modificado para que codifique por una proteina de fusionYPF_NtI"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 1360..1887
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente a los fragmentos N terminal e intermedio de la proteina

<400> 11
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagct caagcttcga attctatggc gagaagtcca     1380

cgtatacgca ttaaggataa tgacaaagcc gaatacgctc gattggtcaa gaatacaaaa     1440

gccaagattg cgagaacgaa gaaaaagtat ggtgtagacc ttaccgctga aattgatata     1500

cctgaccttg attcatttga aacacgggcg cagttcaata agtggaagga acaagcgtcc     1560

tctttcacta accgtgctaa tatgcgttat cagttcgaaa agaatgcata cggtgtggtg     1620

gctagtaaag ctaagatagc tgagattgaa cgtaacacaa aagaggttca gcggttagta     1680

gatgagaaaa tcaaggctat gaaagacaaa gaatactatg caggcggtaa gccgcaaggg     1740

acaattgaac aacggatagc tatgacaagt cctgcacacg ttacaggaat taatagaccc     1800

catgattttg actttagcaa ggtgcgaagc tatagccgtt tgcgaaccct agaagaaagc     1860

atggagatga gaacagaccc tcagtaataa cgggatccac cggatctaga taactgatca     1920

taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc     1980

ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt     2040

ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac     2100

tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc     2160

gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa     2220

taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt     2280

gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg     2340

cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt     2400

ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga     2460

gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg     2520

ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg     2580

cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc     2640

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     2700

tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt     2760

ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc     2820

aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag     2880

gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc     2940

cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa     3000

ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt     3060

gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga     3120

tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag     3180

aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc     3240

cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg     3300

aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc     3360

gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg     3420

ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct     3480

gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg     3540

aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat     3600

ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc     3660

atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg     3720

gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc     3780

tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct     3840

gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat     3900

cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga     3960

cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct     4020

tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg     4080

agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag     4140

gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg     4200

ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc     4260

attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg     4320

aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact     4380

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga     4440

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     4500

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     4560

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     4620

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc     4680

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     4740

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     4800

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     4860

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     4920

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     4980

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     5040

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     5100

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     5160

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     5220

ttaccgccat gcat                                                       5234


<210> 12
<211> 5018
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5018
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_ITPphi29 procede del plasmido pEYFP_C1 modificado para que codifique por una proteina de fusion YPF_I"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 1360..1671
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente al fragmento intermedio de la proteina

<400> 12
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagct caagcttcga attctatgcg ttatcagttc     1380

gaaaagaatg catacggtgt ggtggctagt aaagctaaga tagctgagat tgaacgtaac     1440

acaaaagagg ttcagcggtt agtagatgag aaaatcaagg ctatgaaaga caaagaatac     1500

tatgcaggcg gtaagccgca agggacaatt gaacaacgga tagctatgac aagtcctgca     1560

cacgttacag gaattaatag accccatgat tttgacttta gcaaggtgcg aagctatagc     1620

cgtttgcgaa ccctagaaga aagcatggag atgagaacag accctcagta ataacgggat     1680

ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg     1740

ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg     1800

ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt     1860

tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg     1920

tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt     1980

taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa     2040

gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag     2100

aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt     2160

gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac     2220

cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag     2280

gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg     2340

cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact     2400

tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg     2460

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt     2520

cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag     2580

gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg     2640

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     2700

caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc     2760

attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg     2820

cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag     2880

atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca     2940

ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc     3000

ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc     3060

aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg     3120

ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg     3180

gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct     3240

gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct     3300

acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa     3360

gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa     3420

ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc     3480

gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt     3540

ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct     3600

gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc     3660

gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg     3720

ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg     3780

ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc     3840

tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa     3900

cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata     3960

aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact     4020

ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc     4080

caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag     4140

gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt     4200

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     4260

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     4320

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     4380

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     4440

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     4500

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     4560

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     4620

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     4680

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     4740

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     4800

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     4860

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     4920

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     4980

cccctgattc tgtggataac cgtattaccg ccatgcat                             5018


<210> 13
<211> 4997
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..4997
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_CtTPphi29 procede del plasmido pEYFP_C1 modificado para que codifique por una proteina de fusion YPF_Ct"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 1360..1650
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente al fragmento carboxiterminal de la proteina

<400> 13
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagct caagcttcga attctatgta ttatgaaaag     1380

aaaatgatac agttacagtt aaactttatt aagagcgttg agggtagttt caattcattt     1440

gatgcggcag atgaactgat cgaagaatta aaaaagatac ctcctgatga cttctatgaa     1500

ttgtttctca gaatatcaga aatatccttt gaggaatttg atagtgaggg aaacacagtg     1560

gagaacgtag aaggtaatgt atataaaata ctgtcatact tggaacagta tcgaaggggt     1620

gactttgatc taagcttaaa ggggttctaa taacgggatc caccggatct agataactga     1680

tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc     1740

tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag     1800

cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt     1860

cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta     1920

agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac     1980

caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg     2040

agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa     2100

gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt     2160

tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt     2220

agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga     2280

gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc     2340

gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga     2400

acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa     2460

ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc     2520

tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta     2580

tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag     2640

caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa     2700

ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac     2760

taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt     2820

agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga     2880

ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg     2940

gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg     3000

ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc     3060

ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct     3120

tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa     3180

gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg     3240

gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa     3300

gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat     3360

gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg     3420

agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc     3480

atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac     3540

cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg     3600

gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc     3660

tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag     3720

cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg     3780

gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc     3840

tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg     3900

aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt     3960

ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac     4020

cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg     4080

gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt     4140

actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga     4200

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag     4260

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa     4320

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag     4380

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg     4440

tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat     4500

acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta     4560

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg     4620

gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc     4680

gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa     4740

gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc     4800

tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt     4860

caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct     4920

tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc     4980

gtattaccgc catgcat                                                    4997


<210> 14
<211> 5300
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5300
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_ICtTPphi29 procede del plasmido pEYFP_C1 modificado para que codifique por una proteina de fusion YFP_ICt"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 1360..1953
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente a los fragmentos intermedio y carboxiterminal de la proteina

<400> 14
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagct caagcttcga attctatgat gcgttatcag     1380

ttcgaaaaga atgcatacgg tgtggtggct agtaaagcta agatagctga gattgaacgt     1440

aacacaaaag aggttcagcg gttagtagat gagaaaatca aggctatgaa agacaaagaa     1500

tactatgcag gcggtaagcc gcaagggaca attgaacaac ggatagctat gacaagtcct     1560

gcacacgtta caggaattaa tagaccccat gattttgact ttagcaaggt gcgaagctat     1620

agccgtttgc gaaccctaga agaaagcatg gagatgagaa cagaccctca gtattatgaa     1680

aagaaaatga tacagttaca gttaaacttt attaagagcg ttgagggtag tttcaattca     1740

tttgatgcgg cagatgaact gatcgaagaa ttaaaaaaga tacctcctga tgacttctat     1800

gaattgtttc tcagaatatc agaaatatcc tttgaggaat ttgatagtga gggaaacaca     1860

gtggagaacg tagaaggtaa tgtatataaa atactgtcat acttggaaca gtatcgaagg     1920

ggtgactttg atctaagctt aaaggggttc taataacggg atccaccgga tctagataac     1980

tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac     2040

acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg     2100

cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     2160

tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt     2220

gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt     2280

aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg     2340

ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc     2400

aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca     2460

agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga     2520

tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa     2580

ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc     2640

gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc     2700

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     2760

taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc     2820

agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa     2880

gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc     2940

cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc     3000

taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct     3060

gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga     3120

agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga     3180

tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg     3240

gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc     3300

gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt     3360

gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt     3420

ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc     3480

gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc     3540

atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac     3600

caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag     3660

gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag     3720

gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat     3780

atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg     3840

gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa     3900

tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc     3960

ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc     4020

aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt     4080

tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca     4140

tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac     4200

cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt     4260

cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga     4320

gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt     4380

cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag     4440

gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg     4500

tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact     4560

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     4620

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     4680

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     4740

ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     4800

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     4860

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     4920

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     4980

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     5040

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     5100

atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct     5160

cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg     5220

ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata     5280

accgtattac cgccatgcat                                                 5300


<210> 15
<211> 5424
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5424
<223> /mol_type="DNA"
      /note="Plasmido p2YFP procede del plasmido pEYFP_C1  modificado para que codifique por una proteina de fusion YFP_YFP"
      /organism="Artificial Sequence"

<400> 15
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctggatccac cggatctaga     2100

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc     2160

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt     2220

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     2280

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta     2340

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt     2400

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat     2460

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa     2520

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta     2580

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc     2640

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc     2700

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac     2760

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt     2820

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     2880

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag     2940

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc     3000

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc     3060

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg     3120

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat     3180

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc     3240

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac     3300

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc     3360

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc     3420

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc     3480

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg     3540

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt     3600

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc     3660

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga     3720

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga     3780

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct     3840

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc     3900

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt     3960

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg     4020

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat     4080

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc     4140

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa     4200

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat     4260

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca     4320

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt     4380

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca     4440

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca     4500

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc     4560

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     4620

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     4680

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     4740

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     4800

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     4860

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     4920

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     4980

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     5040

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     5100

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     5160

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     5220

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     5280

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     5340

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     5400

gataaccgta ttaccgccat gcat                                            5424


<210> 16
<211> 6230
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6230
<223> /mol_type="DNA"
      /note="Plasmido p2YFP_TPphi29 procede del plasmido p2YFP modificado para que codifique por una proteina de fusion YFP_YFP_TP"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2883
<223> /gene=Gen 3 del fago phi29 completo

<400> 16
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacaaaagcc     2160

aagattgcga gaacgaagaa aaagtatggt gtagacctta ccgctgaaat tgatatacct     2220

gaccttgatt catttgaaac acgggcgcag ttcaataagt ggaaggaaca agcgtcctct     2280

ttcactaacc gtgctaatat gcgttatcag ttcgaaaaga atgcatacgg tgtggtggct     2340

agtaaagcta agatagctga gattgaacgt aacacaaaag aggttcagcg gttagtagat     2400

gagaaaatca aggctatgaa agacaaagaa tactatgcag gcggtaagcc gcaagggaca     2460

attgaacaac ggatagctat gacaagtcct gcacacgtta caggaattaa tagaccccat     2520

gattttgact ttagcaaggt gcgaagctat agccgtttgc gaaccctaga agaaagcatg     2580

gagatgagaa cagaccctca gtattatgaa aagaaaatga tacagttaca gttaaacttt     2640

attaagagcg ttgagggtag tttcaattca tttgatgcgg cagatgaact gatcgaagaa     2700

ttaaaaaaga tacctcctga tgacttctat gaattgtttc tcagaatatc agaaatatcc     2760

tttgaggaat ttgatagtga gggaaacaca gtggagaacg tagaaggtaa tgtatataaa     2820

atactgtcat acttggaaca gtatcgaagg ggtgactttg atctaagctt aaaggggttc     2880

taataacggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag     2940

aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga     3000

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     3060

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     3120

aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg     3180

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     3240

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     3300

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     3360

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3420

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3480

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3540

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3600

tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata     3660

cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga     3720

aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg     3780

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     3840

caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc     3900

tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc     3960

ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg     4020

aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag     4080

gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat     4140

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca     4200

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg     4260

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg     4320

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact     4380

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct     4440

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg     4500

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt     4560

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc     4620

gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc     4680

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga     4740

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc     4800

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt     4860

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga     4920

gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt     4980

tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg     5040

gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga     5100

ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa     5160

aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc     5220

agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt     5280

cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg     5340

tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa     5400

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     5460

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     5520

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     5580

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     5640

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     5700

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     5760

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     5820

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     5880

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     5940

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     6000

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     6060

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     6120

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     6180

ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat                6230


<210> 17
<211> 5648
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5648
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_EYFP_NtTPphi29 procede del plasmido p2YFP modificado para que codifique por una proteina de fusion YFP_YFP_Nt"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2311
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente al fragmento N terminal de la proteina

<400> 17
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacaaaagcc     2160

aagattgcga gaacgaagaa aaagtatggt gtagacctta ccgctgaaat tgatatacct     2220

gaccttgatt catttgaaac acgggcgcag ttcaataagt ggaaggaaca agcgtcctct     2280

ttcactaacc gtgctaatta ataacgggat ccaccggatc tagataactg atcataatca     2340

gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga     2400

acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg     2460

gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt     2520

ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat     2580

attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc     2640

gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt     2700

ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa     2760

accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg     2820

tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga     2880

cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct     2940

agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat     3000

gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt     3060

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa     3120

atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg     3180

tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca     3240

tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa     3300

gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca     3360

tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt     3420

ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag     3480

gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt     3540

cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta     3600

ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg     3660

tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa     3720

ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct     3780

gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg     3840

caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca     3900

atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat     3960

cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac     4020

gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc     4080

gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa     4140

aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag     4200

gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc     4260

ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt     4320

cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca     4380

acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa     4440

tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct     4500

tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc     4560

gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata     4620

aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg     4680

gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc     4740

cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata     4800

tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt     4860

ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc     4920

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct     4980

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa     5040

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag     5100

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc     5160

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg     5220

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca     5280

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat     5340

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg     5400

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc     5460

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc     5520

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc     5580

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg     5640

ccatgcat                                                              5648


<210> 18
<211> 5540
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5540
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_EYFP_Nt1 a 37TPphi29 procede del plasmido 2YFP modificado para que codifique por una proteina YFP_YFP_Nt1 a 37"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2311
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente a los primeros 37 amino acidos de la proteina

<400> 18
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacaaaagcc     2160

aagattgcga gaacgaagaa aaagtatggt taataacggg atccaccgga tctagataac     2220

tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac     2280

acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg     2340

cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     2400

tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt     2460

gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt     2520

aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg     2580

ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc     2640

aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca     2700

agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga     2760

tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa     2820

ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc     2880

gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc     2940

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     3000

taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc     3060

agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa     3120

gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc     3180

cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc     3240

taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct     3300

gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga     3360

agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga     3420

tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg     3480

gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc     3540

gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt     3600

gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt     3660

ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc     3720

gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc     3780

atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac     3840

caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag     3900

gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag     3960

gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat     4020

atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg     4080

gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa     4140

tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc     4200

ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc     4260

aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt     4320

tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca     4380

tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac     4440

cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt     4500

cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga     4560

gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt     4620

cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag     4680

gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg     4740

tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact     4800

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     4860

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     4920

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     4980

ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     5040

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     5100

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     5160

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     5220

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     5280

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     5340

atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct     5400

cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg     5460

ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata     5520

accgtattac cgccatgcat                                                 5540


<210> 19
<211> 5504
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5504
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_EYFP_Nt1 a 24TPphi29 procede del plasmido p2YFP modificado para que codifique por una proteina de fusion YFP_YFP_Nt1 a 24"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2157
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente a los primeros 24 amino acidos de la proteina

<400> 19
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacataataa     2160

cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt     2220

tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa     2280

ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca     2340

caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca     2400

tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat     2460

ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa     2520

tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta     2580

ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca     2640

ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat     2700

cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg     2760

agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc     2820

acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt     2880

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     2940

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg     3000

aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt     3060

ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca     3120

ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt     3180

agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt     3240

ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg     3300

cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt     3360

gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg     3420

cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag     3480

acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt     3540

tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta     3600

tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg     3660

ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt     3720

gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat     3780

ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg     3840

atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca     3900

gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc     3960

catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc     4020

gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat     4080

attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc     4140

gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga     4200

ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt     4260

ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga     4320

tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa     4380

ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac     4440

agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct     4500

ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct     4560

tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg     4620

cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc     4680

atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc     4740

cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt     4800

cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac     4860

cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct     4920

tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact     4980

tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg     5040

ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata     5100

aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga     5160

cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag     5220

ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg     5280

agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac     5340

ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca     5400

acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg     5460

cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat                      5504


<210> 20
<211> 5501
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5501
<223> /mol_type="DNA"
      /note="Plasmido pEYFP_EYFP_Nt14 a 37TPphi29 procede del plasmido 2YFP modificado para que codifique por una proteina de fusion YFP_YFP_Nt14 a 37"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2154
<223> /gene=Fragmento del gen 3 del fago phi29 correspondiente a los amino acidos 14 al 37 de la protein"

<400> 20
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggccga atacgctcga     2100

ttggtcaaga atacaaaagc caagattgcg agaacgaaga aaaagtatgg ttaataacgg     2160

gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac     2220

ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg     2280

ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa     2340

atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca     2400

atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt     2460

tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca     2520

aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta     2580

aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta     2640

cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg     2700

aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga     2760

aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg     2820

ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc     2880

acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat     2940

atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag     3000

agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc     3060

caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt     3120

gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     3180

cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg     3240

cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct     3300

cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca     3360

aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac     3420

gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca     3480

atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt     3540

gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg     3600

tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga     3660

agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct     3720

cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg     3780

gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg     3840

gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc     3900

gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat     3960

ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac     4020

tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt     4080

gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct     4140

cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc     4200

tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca     4260

ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga     4320

tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg     4380

aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga     4440

ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc     4500

actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt     4560

ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg     4620

caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt     4680

tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt     4740

aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt     4800

gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag     4860

cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca     4920

gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca     4980

agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg     5040

ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg     5100

cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct     5160

acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga     5220

gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc     5280

ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg     5340

agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg     5400

cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt     5460

tatcccctga ttctgtggat aaccgtatta ccgccatgca t                         5501


<210> 21
<211> 6230
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6230
<223> /mol_type="DNA"
      /note="Plasmido p2YFP_TPphi29_K27A procede del plasmido 2YFP modificado para que codifique por una preoteina de fusion YFP_YFP_TP 
con una mutacion K27A en la secuencia TP"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2883
<223> /gene=Gen 3 del fago phi29 completo con una mutacion para cambiar un triplete aaa por gcg lo que cambia la lisina en posicion 27 por una alanina

<400> 21
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacaaaagcc     2160

gcgattgcga gaacgaagaa aaagtatggt gtagacctta ccgctgaaat tgatatacct     2220

gaccttgatt catttgaaac acgggcgcag ttcaataagt ggaaggaaca agcgtcctct     2280

ttcactaacc gtgctaatat gcgttatcag ttcgaaaaga atgcatacgg tgtggtggct     2340

agtaaagcta agatagctga gattgaacgt aacacaaaag aggttcagcg gttagtagat     2400

gagaaaatca aggctatgaa agacaaagaa tactatgcag gcggtaagcc gcaagggaca     2460

attgaacaac ggatagctat gacaagtcct gcacacgtta caggaattaa tagaccccat     2520

gattttgact ttagcaaggt gcgaagctat agccgtttgc gaaccctaga agaaagcatg     2580

gagatgagaa cagaccctca gtattatgaa aagaaaatga tacagttaca gttaaacttt     2640

attaagagcg ttgagggtag tttcaattca tttgatgcgg cagatgaact gatcgaagaa     2700

ttaaaaaaga tacctcctga tgacttctat gaattgtttc tcagaatatc agaaatatcc     2760

tttgaggaat ttgatagtga gggaaacaca gtggagaacg tagaaggtaa tgtatataaa     2820

atactgtcat acttggaaca gtatcgaagg ggtgactttg atctaagctt aaaggggttc     2880

taataacggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag     2940

aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga     3000

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     3060

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     3120

aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg     3180

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     3240

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     3300

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     3360

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3420

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3480

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3540

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3600

tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata     3660

cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga     3720

aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg     3780

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     3840

caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc     3900

tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc     3960

ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg     4020

aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag     4080

gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat     4140

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca     4200

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg     4260

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg     4320

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact     4380

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct     4440

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg     4500

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt     4560

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc     4620

gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc     4680

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga     4740

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc     4800

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt     4860

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga     4920

gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt     4980

tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg     5040

gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga     5100

ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa     5160

aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc     5220

agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt     5280

cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg     5340

tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa     5400

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     5460

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     5520

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     5580

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     5640

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     5700

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     5760

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     5820

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     5880

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     5940

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     6000

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     6060

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     6120

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     6180

ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat                6230


<210> 22
<211> 6230
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6230
<223> /mol_type="DNA"
      /note="Plasmido p2YFP_TPphi29_K2527A procede del plasmido 2YFP modificado para que codifique por una proteina de fusion YFP_YFP_TP"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2883
<223> /gene=Gen 3 del fago phi29 completo con una mutacion para cambiar dos tripletes aaa por gcg lo que cambia las lisinas en posiciones 25 y 27 por una alanina

<400> 22
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacagcggcc     2160

gcgattgcga gaacgaagaa aaagtatggt gtagacctta ccgctgaaat tgatatacct     2220

gaccttgatt catttgaaac acgggcgcag ttcaataagt ggaaggaaca agcgtcctct     2280

ttcactaacc gtgctaatat gcgttatcag ttcgaaaaga atgcatacgg tgtggtggct     2340

agtaaagcta agatagctga gattgaacgt aacacaaaag aggttcagcg gttagtagat     2400

gagaaaatca aggctatgaa agacaaagaa tactatgcag gcggtaagcc gcaagggaca     2460

attgaacaac ggatagctat gacaagtcct gcacacgtta caggaattaa tagaccccat     2520

gattttgact ttagcaaggt gcgaagctat agccgtttgc gaaccctaga agaaagcatg     2580

gagatgagaa cagaccctca gtattatgaa aagaaaatga tacagttaca gttaaacttt     2640

attaagagcg ttgagggtag tttcaattca tttgatgcgg cagatgaact gatcgaagaa     2700

ttaaaaaaga tacctcctga tgacttctat gaattgtttc tcagaatatc agaaatatcc     2760

tttgaggaat ttgatagtga gggaaacaca gtggagaacg tagaaggtaa tgtatataaa     2820

atactgtcat acttggaaca gtatcgaagg ggtgactttg atctaagctt aaaggggttc     2880

taataacggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag     2940

aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga     3000

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     3060

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     3120

aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg     3180

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     3240

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     3300

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     3360

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3420

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3480

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3540

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3600

tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata     3660

cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga     3720

aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg     3780

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     3840

caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc     3900

tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc     3960

ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg     4020

aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag     4080

gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat     4140

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca     4200

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg     4260

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg     4320

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact     4380

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct     4440

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg     4500

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt     4560

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc     4620

gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc     4680

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga     4740

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc     4800

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt     4860

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga     4920

gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt     4980

tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg     5040

gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga     5100

ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa     5160

aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc     5220

agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt     5280

cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg     5340

tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa     5400

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     5460

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     5520

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     5580

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     5640

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     5700

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     5760

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     5820

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     5880

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     5940

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     6000

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     6060

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     6120

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     6180

ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat                6230


<210> 23
<211> 6230
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6230
<223> /mol_type="DNA"
      /note="Plasmido p2YFP_TPphi29_R19A procede del plasmido 2YFP modificado para que codifique por una proteina de fusion YFP_YFP_TP"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2883
<223> /gene=Gen 3 del fago phi29 completo con una mutacion para cambiar dos tripletes cga por gca lo que cambia la arginina en posicion 19 por una alanina

<400> 23
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacaaaagcc     2160

aagattgcga gaacgaagaa aaagtatggt gtagacctta ccgctgaaat tgatatacct     2220

gaccttgatt catttgaaac acgggcgcag ttcaataagt ggaaggaaca agcgtcctct     2280

ttcactaacc gtgctaatat gcgttatcag ttcgaaaaga atgcatacgg tgtggtggct     2340

agtaaagcta agatagctga gattgaacgt aacacaaaag aggttcagcg gttagtagat     2400

gagaaaatca aggctatgaa agacaaagaa tactatgcag gcggtaagcc gcaagggaca     2460

attgaacaac ggatagctat gacaagtcct gcacacgtta caggaattaa tagaccccat     2520

gattttgact ttagcaaggt gcgaagctat agccgtttgc gaaccctaga agaaagcatg     2580

gagatgagaa cagaccctca gtattatgaa aagaaaatga tacagttaca gttaaacttt     2640

attaagagcg ttgagggtag tttcaattca tttgatgcgg cagatgaact gatcgaagaa     2700

ttaaaaaaga tacctcctga tgacttctat gaattgtttc tcagaatatc agaaatatcc     2760

tttgaggaat ttgatagtga gggaaacaca gtggagaacg tagaaggtaa tgtatataaa     2820

atactgtcat acttggaaca gtatcgaagg ggtgactttg atctaagctt aaaggggttc     2880

taataacggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag     2940

aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga     3000

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     3060

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     3120

aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg     3180

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     3240

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     3300

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     3360

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3420

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3480

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3540

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3600

tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata     3660

cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga     3720

aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg     3780

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     3840

caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc     3900

tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc     3960

ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg     4020

aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag     4080

gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat     4140

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca     4200

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg     4260

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg     4320

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact     4380

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct     4440

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg     4500

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt     4560

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc     4620

gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc     4680

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga     4740

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc     4800

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt     4860

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga     4920

gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt     4980

tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg     5040

gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga     5100

ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa     5160

aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc     5220

agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt     5280

cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg     5340

tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa     5400

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     5460

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     5520

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     5580

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     5640

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     5700

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     5760

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     5820

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     5880

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     5940

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     6000

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     6060

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     6120

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     6180

ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat                6230


<210> 24
<211> 6230
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6230
<223> /mol_type="DNA"
      /note="Plasmido p2YFP_TPphi29_K32334A procedente del plasmido 2YFP modificado para que codifique por una proteina de fusion YFP_YFP_TP"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2883
<223> /gene=Gen 3 del fago phi29 completo con una mutacion para cambiar tres tripletes consecutivos aag aaa aag por gcg gca gcg lo que cambia las lisinas en posiciones 32 33 y 34 por una alanina

<400> 24
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgag aagtccacgt     2100

atacgcatta aggataatga caaagccgaa tacgctcgat tggtcaagaa tacaaaagcc     2160

aagattgcga gaacggcggc agcgtatggt gtagacctta ccgctgaaat tgatatacct     2220

gaccttgatt catttgaaac acgggcgcag ttcaataagt ggaaggaaca agcgtcctct     2280

ttcactaacc gtgctaatat gcgttatcag ttcgaaaaga atgcatacgg tgtggtggct     2340

agtaaagcta agatagctga gattgaacgt aacacaaaag aggttcagcg gttagtagat     2400

gagaaaatca aggctatgaa agacaaagaa tactatgcag gcggtaagcc gcaagggaca     2460

attgaacaac ggatagctat gacaagtcct gcacacgtta caggaattaa tagaccccat     2520

gattttgact ttagcaaggt gcgaagctat agccgtttgc gaaccctaga agaaagcatg     2580

gagatgagaa cagaccctca gtattatgaa aagaaaatga tacagttaca gttaaacttt     2640

attaagagcg ttgagggtag tttcaattca tttgatgcgg cagatgaact gatcgaagaa     2700

ttaaaaaaga tacctcctga tgacttctat gaattgtttc tcagaatatc agaaatatcc     2760

tttgaggaat ttgatagtga gggaaacaca gtggagaacg tagaaggtaa tgtatataaa     2820

atactgtcat acttggaaca gtatcgaagg ggtgactttg atctaagctt aaaggggttc     2880

taataacggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag     2940

aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga     3000

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     3060

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     3120

aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg     3180

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     3240

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     3300

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     3360

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3420

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3480

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3540

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3600

tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata     3660

cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga     3720

aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg     3780

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     3840

caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc     3900

tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc     3960

ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg     4020

aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag     4080

gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat     4140

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca     4200

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg     4260

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg     4320

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact     4380

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct     4440

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg     4500

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt     4560

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc     4620

gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc     4680

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga     4740

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc     4800

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt     4860

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga     4920

gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt     4980

tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg     5040

gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga     5100

ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa     5160

aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc     5220

agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt     5280

cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg     5340

tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa     5400

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     5460

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     5520

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     5580

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     5640

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     5700

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     5760

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     5820

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     5880

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     5940

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     6000

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     6060

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     6120

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     6180

ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat                6230


<210> 25
<211> 6381
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6381
<223> /mol_type="DNA"
      /note="Plasmido p2YFP_TPphiNfprocede del plasmido 2YFP modificado para que codifique por una proteina de fusion YFP_YFP_TPNf"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..3034
<223> /gene=Gen 3 del fago Nf completo

<400> 25
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgatgt tggcagatat aacgttctac     2100

agtaatgtag aaaagagagg tttccatgcg tggttaaagg ggtgtaacac aacatggcaa     2160

gaaattcacg tatacgcatt acgaacaatg acaaagcctt gtacgcaaaa ctggtcaaga     2220

atacgaaagc caaaattagt cgaacgaaga aaaagtatgg tatagaccta agcaatgaga     2280

ttgagttgcc gccattggag tcatttcaaa ctcgtgaaga gtttaataag tggaagcaaa     2340

aacaagaatc atttacgaat cgtgctaatc aaaattatca gtttgttaaa aataaatatg     2400

gaattgtggc tagtaaagcg aaaattaatg agattgcgaa gaacacaaaa gaagcccaaa     2460

gaatcgttga tgaacagagg gaagaaatag aggacaagcc ttttatctct ggtggtaaac     2520

aacagggtac tgttggtcaa cgtatgcaaa tactcagtcc ttcacaggtt acaggtatca     2580

gtagaccaag tgattttaac tttgatgatg tgagaagcta tgcaaggttg agaaccttgg     2640

aagaaggtat ggcagaaaaa gcatccccag attactacga tagacgtatg acacagatgc     2700

accaaaactt cattgaaata gttgaaaaaa gttttaactc tgattggttg agtgatgaat     2760

tggttgagag attaaagaaa ataccgccag atgatttttt tgaattatat ctcatgtttg     2820

atgaaatatc gtttgagtat tttgatagtg aaggtgagga tgtggaagca tcggaagcta     2880

tgttgaataa gatacattca tatctcgatc gctatgagcg aggggacgtg aatcttgact     2940

taaaggggtt ttaaagcacc gttgaagggt tgaaagtata tgtctaggaa aatgtttagt     3000

tgtgactttg agacgactac aaagttagac gatttaacgg gatccaccgg atctagataa     3060

ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca     3120

cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt     3180

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt     3240

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat     3300

tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt     3360

taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg     3420

gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt     3480

caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc     3540

aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg     3600

atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa     3660

aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc     3720

cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg     3780

cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca     3840

ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac     3900

cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga     3960

agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc     4020

ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc     4080

ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc     4140

tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag     4200

aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg     4260

atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg     4320

ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc     4380

cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg     4440

tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt     4500

tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg     4560

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat     4620

catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca     4680

ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca     4740

ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa     4800

ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa     4860

tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc     4920

ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga     4980

atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc     5040

cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac     5100

caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg     5160

ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc     5220

atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata     5280

ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg     5340

tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg     5400

agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt     5460

tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca     5520

ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag     5580

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     5640

tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     5700

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     5760

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     5820

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     5880

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     5940

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     6000

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     6060

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     6120

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     6180

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     6240

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     6300

gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat     6360

aaccgtatta ccgccatgca t                                               6381

<210> 26
<211> 6116
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..6116
<223> /mol_type="DNA"
      /note=Plasmido p2YFP_TPCp1 procede del plasmido 2YFP modificado para que codifique por una proteina de fusion YFP_YFP_TPCp1
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2768
<223> /gene=Gen 4 del fago Cp1 completo

<400> 26
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgatgg ctttaacacc aaaacaaagg     2100

aaggtaagac gggactattt aaccagaaag aaaagaacac tacaacagca gggcgcctct     2160

aatgctgaaa taaaagcttt tatgggcggt cggtggaatt ttgcaggaat gagtgataag     2220

gcgctagaac gtgcctatga tgagattaaa agcaaaggtc gtactcaagt atttggcaat     2280

catgtttaca ctagtgacta tgtaaaaaaa gctaaggcat ggtacgggga taagttttct     2340

gtagaaaagc tgactcaagg ttttcgctct tctcaacgct cagagttgaa ccgctttcat     2400

tcagttaagg aagtcaaaga ataccgctca gaacgtgaca gagaagccaa agaacgctat     2460

atacaagcgc tggaagaaat gcactataat acaagagacg caggcaacaa ggcgcaagaa     2520

aaagccttta agtctatgat ttcacgcata aggcgaatga gtgccagcaa ctttggggca     2580

tttctaacag gcggacggtc tgacaaggtt tcatttgata atgtcatggt ttttgtagac     2640

acggacggta aggaaacggc ttttgaattt caggacagtc tagcccgtga aatccttgat     2700

aatgtagata agttttccaa gcaatttgtt tctgatatga gaagacgaaa gaagcgaggg     2760

aagaagtgat aacgggatcc accggatcta gataactgat cataatcagc cataccacat     2820

ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata     2880

aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa     2940

gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt     3000

tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa     3060

ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa     3120

atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac     3180

aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag     3240

ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt     3300

aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg     3360

gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca     3420

agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag     3480

ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc     3540

taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa     3600

tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag     3660

ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt     3720

agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca     3780

tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa     3840

ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag     3900

aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag     3960

gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa     4020

caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac     4080

tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg     4140

cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag     4200

gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt     4260

gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg     4320

tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg     4380

catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga     4440

gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag     4500

gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat     4560

ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt     4620

tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg     4680

gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt     4740

tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc     4800

ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac     4860

gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg     4920

acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta     4980

gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg     5040

caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc     5100

ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc     5160

gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag     5220

ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg     5280

atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca     5340

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga     5400

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa     5460

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga     5520

aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt     5580

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt     5640

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat     5700

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct     5760

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca     5820

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag     5880

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc     5940

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga     6000

aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca     6060

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat         6116

<210> 27
<211> 6158
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6158
<223> /mol_type="DNA"
      /note=Plasmido 2YFP_TPBam35 procede del plasmido 2YFP modificado para que codifique una proteina de fusion YFP_YFP_TPBam35
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2811
<223> /gene=Gen 4 del fago Bam35 completo

<400> 27
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgatgg caaataaacg gttaaagaag     2100

aaactagaaa ccaaacgtaa aaagtcgtta cttgtttcgg aaggttatag taagaaagaa     2160

acaaagaagc tgaaaggtag agagttagaa actgtttata aaaagaaagc gcataatagg     2220

aaaaatagag agcgcgctag ggaaatagca aacctagcaa aacagtgggg tctatcaccg     2280

agtaagtata actcttggaa aaagttactt cccgaaatag agagaattaa aaaagaacaa     2340

gatagggaag caccttttct attaatatac tatcaagact ttacaggtga gacagatagt     2400

aagtttatat acgattttaa aaagagaaat aatacaagat cacgatctca aattacagaa     2460

tccataatag gatggttaca gaatgctcat aataaactct ttttgggaag ggttgctata     2520

cgtatagttc caaagcgtga tgtttcgaaa acgaatacac tttggagaaa tcatggttat     2580

gtaaagatat atgaagggca agggaaagag ttatcaaaac tattaacggc tatagaaacg     2640

attatggtcg gtgtgtatga tgtaaaagag cgtgacaaat atttaaagga attagtagct     2700

aagctacgtt ctttacctta tgaaaaggct aaaaagaacg caaaggagat acaaaagata     2760

tacgatacaa agtcgtataa gaaagaaagt tgggataatg atgactacta ctaacgggat     2820

ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg     2880

ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg     2940

ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt     3000

tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg     3060

tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt     3120

taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa     3180

gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag     3240

aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt     3300

gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac     3360

cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag     3420

gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg     3480

cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact     3540

tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg     3600

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt     3660

cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag     3720

gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg     3780

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     3840

caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc     3900

attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg     3960

cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag     4020

atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca     4080

ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc     4140

ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc     4200

aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg     4260

ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg     4320

gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct     4380

gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct     4440

acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa     4500

gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa     4560

ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc     4620

gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt     4680

ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct     4740

gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc     4800

gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg     4860

ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg     4920

ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc     4980

tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa     5040

cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata     5100

aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact     5160

ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc     5220

caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag     5280

gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt     5340

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     5400

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     5460

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     5520

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     5580

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     5640

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     5700

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     5760

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     5820

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     5880

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     5940

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     6000

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     6060

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     6120

cccctgattc tgtggataac cgtattaccg ccatgcat                             6158

<210> 28
<211> 6204
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..6204
<223> /mol_type="DNA"
      /note="Plasmido p2YFP_TPPRD1 procede del plasmido 2YFP modificado para que codifique por una proteina de fusion YFP_YFP_TPPRD1"
      /organism="Artificial Sequence"

<220> 
<221> gene
<222> 2077..2862
<223> /gene=Gen 8 del fago PRD1 completo

<400> 28
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      660

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      720

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      780

ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc      840

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      900

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      960

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac     1020

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac     1080

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc     1140

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     1200

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc     1260

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag     1320

ctgtacaagt ccggactcag atctcgagcc atggtgagca agggcgagga gctgttcacc     1380

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1440

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1500

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1560

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1620

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1680

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1740

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1800

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1860

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1920

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1980

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     2040

actctcggca tggacgagct gtacaagcaa gcttcgaatt ctatggcgaa gaaaaaacca     2100

gtagaaaaaa atgggcttgt ttataaagag tttcaaaaac aagtttcaaa tttgaagaaa     2160

gccggactaa tccctaaaac ccttgacgtg cgaaaagtca agccaacaaa acactataaa     2220

ggattggtaa gcaaatataa agacgttgca acagggggcg ctaaacttgc agcaatccct     2280

aaccccgccg ttattgaaac gcttgaagcg cggggcgaat ccatcattaa gaaaggcggc     2340

aaggcgtatc tgaaagcccg ccagcaaata aaccagcgcg ggcaaattgt aaaccccttt     2400

acggttcgcg taaccaaacg cggcgaagtg gtgcgccgct accgcaagac taccccggaa     2460

ggcaagcccg tttatatcac gcaacgggaa ttgcctatta agtttgaaaa tatggaacag     2520

tggcttactg aattaaaggc cgctggtttt caattgcaac cgggcgaaca aatctatttc     2580

acttttaacg gcaactattc ccgccgtacc tatacgtcat ttgatgaagc gttcaataaa     2640

tttatgacgt atgacattat tattgatgcg gtggccggaa aattaaaagt agaagatgaa     2700

gccgatttag ttaagtcggt aggctttcaa cgtatcagcg gccccgaagc caaggcgtat     2760

aaccgtaacc gtattgtatt gcctgaaatg caatttagcc aagcggctaa aaagaaatac     2820

aagcgccgtc aaaaacgcgg ctatggcagc aagggggttt aaggatccac cggatctaga     2880

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc     2940

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt     3000

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     3060

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta     3120

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt     3180

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat     3240

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa     3300

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta     3360

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc     3420

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc     3480

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac     3540

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt     3600

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     3660

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag     3720

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc     3780

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc     3840

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg     3900

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat     3960

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc     4020

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac     4080

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc     4140

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc     4200

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc     4260

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg     4320

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt     4380

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc     4440

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga     4500

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga     4560

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct     4620

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc     4680

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt     4740

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg     4800

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat     4860

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc     4920

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa     4980

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat     5040

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca     5100

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt     5160

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca     5220

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca     5280

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc     5340

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     5400

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     5460

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     5520

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     5580

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     5640

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     5700

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     5760

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     5820

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     5880

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     5940

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     6000

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     6060

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     6120

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     6180

gataaccgta ttaccgccat gcat                                            6204


<210> 29
<211> 5302
<212> DNA
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..5302
<223> /mol_type="DNA"
      /note="Plasmido peYFPORBAE descrito en PCTES2012070121"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 5021..5214
<223> /note="Origen derecho del genoma del fago phi29"

<220> 
<221> misc_feature
<222> 4792..4982
<223> /note="Origen izquierdo del genoma del fago phi29"

<400> 29
atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata       60

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      120

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      180

gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      240

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      300

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      360

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      420

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      480

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      540

ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca      600

gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg      660

gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc      720

gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat      780

gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc      840

tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac      900

cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc      960

accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc     1020

gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc     1080

ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag     1140

cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg     1200

cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc     1260

gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat     1320

cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg     1380

tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt     1440

tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa     1500

ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca     1560

caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca     1620

tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat     1680

ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa     1740

tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta     1800

ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca     1860

ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat     1920

cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg     1980

agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc     2040

acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt     2100

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     2160

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg     2220

aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt     2280

ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca     2340

ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt     2400

agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt     2460

ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg     2520

cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt     2580

gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg     2640

cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag     2700

acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt     2760

tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta     2820

tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg     2880

ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt     2940

gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat     3000

ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg     3060

atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca     3120

gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc     3180

catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc     3240

gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat     3300

attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc     3360

gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga     3420

ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt     3480

ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga     3540

tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa     3600

ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac     3660

agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct     3720

ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct     3780

tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg     3840

cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc     3900

atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc     3960

cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt     4020

cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac     4080

cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct     4140

tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact     4200

tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg     4260

ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata     4320

aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga     4380

cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag     4440

ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg     4500

agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac     4560

ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca     4620

acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg     4680

cgttatcccc tgcgtctctg gattggtggt ggtggtgctc gagtgcggcc gcaagcttgc     4740

atgcaggcct ctgcagtcga cgggcccggg atccgatcca atcagtggct gtgcgacaca     4800

gacgaagcgc taaaacgtgg gattctgtgt cgttttatgt tgttcattga caaacctatc     4860

tagtaggtct atagattgat atattaagta gtagtttccc tccctgatca tggtatgccg     4920

aggatcgacg gattatgtcg atattaggag aatggtatca tgtgagggtg ggggcttact     4980

tttctcctaa ttatttgact gtcgtatctg tcatcaacca aaagtagggt acagcgacaa     5040

catacaccat ttccccattg accgactatc ttcgacaaga atctaacaac taaatcacga     5100

ctatatacct atactattta ttatcatcaa tttgtcgaaa agggtagaca aactatcgtt     5160

taacatgtta tactataata gaagtaaggt aataagacaa ccaatcatag gaggcacgag     5220

attgatctag atgcattcgc gaggtaccga gctcgaattc ggatcccgac ccatttgctg     5280

tccaccagtc atgctagcca ta                                              5302









