                         SEQUENCE LISTING

<110>  TRANSGENE SA
 
<120>  MYCOBACTERIUM RESUSCITATION PROMOTING FACTOR FOR USE AS ADJUVANT

<130>  363351D32653

<150>  EP 12305826.5
<151>  2012-07-10

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  349
<212>  PRT
<213>  artificial sequence

<220>
<223>  RpfB and RpfD fusion design

<400>  1

Thr Val Asp Gly Thr Ala Met Arg Val Thr Thr Met Lys Ser Arg Val 
1               5                   10                  15      


Ile Asp Ile Val Glu Glu Asn Gly Phe Ser Val Asp Asp Arg Asp Asp 
            20                  25                  30          


Leu Tyr Pro Ala Ala Gly Val Gln Val His Asp Ala Asp Thr Ile Val 
        35                  40                  45              


Leu Arg Arg Ser Arg Pro Leu Gln Ile Ser Leu Asp Gly His Asp Ala 
    50                  55                  60                  


Lys Gln Val Trp Thr Thr Ala Ser Thr Val Asp Glu Ala Leu Ala Gln 
65                  70                  75                  80  


Leu Ala Met Thr Asp Thr Ala Pro Ala Ala Ala Ser Arg Ala Ser Arg 
                85                  90                  95      


Val Pro Leu Ser Gly Met Ala Leu Pro Val Val Ser Ala Lys Thr Val 
            100                 105                 110         


Gln Leu Asn Asp Gly Gly Leu Val Arg Thr Val His Leu Pro Ala Pro 
        115                 120                 125             


Asn Val Ala Gly Leu Leu Ser Ala Ala Gly Val Pro Leu Leu Gln Ser 
    130                 135                 140                 


Asp His Val Val Pro Ala Ala Thr Ala Pro Ile Val Glu Gly Met Gln 
145                 150                 155                 160 


Ile Gln Val Thr Arg Asn Arg Ile Lys Lys Val Thr Glu Arg Leu Pro 
                165                 170                 175     


Leu Pro Pro Asn Ala Arg Arg Val Glu Asp Pro Glu Met Asn Met Ser 
            180                 185                 190         


Arg Glu Val Val Glu Asp Pro Gly Val Pro Gly Thr Gln Asp Val Thr 
        195                 200                 205             


Phe Ala Val Ala Glu Val Asn Gly Val Glu Thr Gly Arg Leu Pro Val 
    210                 215                 220                 


Ala Asn Val Val Val Thr Pro Ala His Glu Ala Val Val Arg Val Gly 
225                 230                 235                 240 


Thr Lys Pro Gly Thr Glu Val Pro Pro Val Ile Asp Gly Ser Ile Trp 
                245                 250                 255     


Asp Ala Ile Ala Gln Cys Lys Ser Gly Gly Asn Trp Ala Ala Asn Thr 
            260                 265                 270         


Gly Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Ala Trp Asp 
        275                 280                 285             


Ser Asn Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln 
    290                 295                 300                 


Ile Glu Val Ala Asp Asn Ile Met Lys Thr Ala Gly Pro Gly Ala Trp 
305                 310                 315                 320 


Pro Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu 
                325                 330                 335     


Thr His Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly 
            340                 345                 


<210>  2
<211>  463
<212>  PRT
<213>  artificial sequence

<220>
<223>  hybrid RpfB and RpfD protein

<400>  2

Met Val Pro Gln Ala Leu Leu Leu Val Pro Leu Leu Gly Phe Ser Leu 
1               5                   10                  15      


Cys Phe Gly Lys Phe Pro Ile Asp Tyr Lys Asp Asp Asp Asp Lys Thr 
            20                  25                  30          


Val Asp Gly Thr Ala Met Arg Val Thr Thr Met Lys Ser Arg Val Ile 
        35                  40                  45              


Asp Ile Val Glu Glu Asn Gly Phe Ser Val Asp Asp Arg Asp Asp Leu 
    50                  55                  60                  


Tyr Pro Ala Ala Gly Val Gln Val His Asp Ala Asp Thr Ile Val Leu 
65                  70                  75                  80  


Arg Arg Ser Arg Pro Leu Gln Ile Ser Leu Asp Gly His Asp Ala Lys 
                85                  90                  95      


Gln Val Trp Thr Thr Ala Ser Thr Val Asp Glu Ala Leu Ala Gln Leu 
            100                 105                 110         


Ala Met Thr Asp Thr Ala Pro Ala Ala Ala Ser Arg Ala Ser Arg Val 
        115                 120                 125             


Pro Leu Ser Gly Met Ala Leu Pro Val Val Ser Ala Lys Thr Val Gln 
    130                 135                 140                 


Leu Asn Asp Gly Gly Leu Val Arg Thr Val His Leu Pro Ala Pro Asn 
145                 150                 155                 160 


Val Ala Gly Leu Leu Ser Ala Ala Gly Val Pro Leu Leu Gln Ser Asp 
                165                 170                 175     


His Val Val Pro Ala Ala Thr Ala Pro Ile Val Glu Gly Met Gln Ile 
            180                 185                 190         


Gln Val Thr Arg Asn Arg Ile Lys Lys Val Thr Glu Arg Leu Pro Leu 
        195                 200                 205             


Pro Pro Asn Ala Arg Arg Val Glu Asp Pro Glu Met Asn Met Ser Arg 
    210                 215                 220                 


Glu Val Val Glu Asp Pro Gly Val Pro Gly Thr Gln Asp Val Thr Phe 
225                 230                 235                 240 


Ala Val Ala Glu Val Asn Gly Val Glu Thr Gly Arg Leu Pro Val Ala 
                245                 250                 255     


Asn Val Val Val Thr Pro Ala His Glu Ala Val Val Arg Val Gly Thr 
            260                 265                 270         


Lys Pro Gly Thr Glu Val Pro Pro Val Ile Asp Gly Ser Ile Trp Asp 
        275                 280                 285             


Ala Ile Ala Gln Cys Lys Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly 
    290                 295                 300                 


Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Ala Trp Asp Ser 
305                 310                 315                 320 


Asn Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile 
                325                 330                 335     


Glu Val Ala Asp Asn Ile Met Lys Thr Ala Gly Pro Gly Ala Trp Pro 
            340                 345                 350         


Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr 
        355                 360                 365             


His Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly Glu Gln Lys Leu 
    370                 375                 380                 


Ile Ser Glu Glu Asp Leu Ser Tyr Val Leu Leu Ser Ala Gly Thr Leu 
385                 390                 395                 400 


Ile Ala Leu Met Leu Ile Ile Phe Leu Ile Thr Cys Cys Lys Arg Val 
                405                 410                 415     


Asp Arg Pro Glu Ser Thr Gln Arg Ser Leu Arg Gly Thr Gly Arg Asn 
            420                 425                 430         


Val Ser Val Thr Ser Gln Ser Gly Lys Phe Ile Ser Ser Trp Glu Ser 
        435                 440                 445             


His Lys Ser Gly Gly Glu Thr Arg Leu His His His His His His 
    450                 455                 460             


<210>  3
<211>  374
<212>  PRT
<213>  artificial sequence

<220>
<223>  hybrid protein between Mtb RpfB and RpfD

<400>  3

Met Asp Tyr Lys Asp Asp Asp Asp Lys Thr Val Asp Gly Thr Ala Met 
1               5                   10                  15      


Arg Val Thr Thr Met Lys Ser Arg Val Ile Asp Ile Val Glu Glu Asn 
            20                  25                  30          


Gly Phe Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro Ala Ala Gly Val 
        35                  40                  45              


Gln Val His Asp Ala Asp Thr Ile Val Leu Arg Arg Ser Arg Pro Leu 
    50                  55                  60                  


Gln Ile Ser Leu Asp Gly His Asp Ala Lys Gln Val Trp Thr Thr Ala 
65                  70                  75                  80  


Ser Thr Val Asp Glu Ala Leu Ala Gln Leu Ala Met Thr Asp Thr Ala 
                85                  90                  95      


Pro Ala Ala Ala Ser Arg Ala Ser Arg Val Pro Leu Ser Gly Met Ala 
            100                 105                 110         


Leu Pro Val Val Ser Ala Lys Thr Val Gln Leu Asn Asp Gly Gly Leu 
        115                 120                 125             


Val Arg Thr Val His Leu Pro Ala Pro Asn Val Ala Gly Leu Leu Ser 
    130                 135                 140                 


Ala Ala Gly Val Pro Leu Leu Gln Ser Asp His Val Val Pro Ala Ala 
145                 150                 155                 160 


Thr Ala Pro Ile Val Glu Gly Met Gln Ile Gln Val Thr Arg Asn Arg 
                165                 170                 175     


Ile Lys Lys Val Thr Glu Arg Leu Pro Leu Pro Pro Asn Ala Arg Arg 
            180                 185                 190         


Val Glu Asp Pro Glu Met Asn Met Ser Arg Glu Val Val Glu Asp Pro 
        195                 200                 205             


Gly Val Pro Gly Thr Gln Asp Val Thr Phe Ala Val Ala Glu Val Asn 
    210                 215                 220                 


Gly Val Glu Thr Gly Arg Leu Pro Val Ala Asn Val Val Val Thr Pro 
225                 230                 235                 240 


Ala His Glu Ala Val Val Arg Val Gly Thr Lys Pro Gly Thr Glu Val 
                245                 250                 255     


Pro Pro Val Ile Asp Gly Ser Ile Trp Asp Ala Ile Ala Gln Cys Lys 
            260                 265                 270         


Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu Tyr Gly Gly 
        275                 280                 285             


Leu Gln Ile Ser Gln Ala Ala Trp Asp Ser Asn Gly Gly Val Gly Ser 
    290                 295                 300                 


Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu Val Ala Asp Asn Ile 
305                 310                 315                 320 


Met Lys Thr Ala Gly Pro Gly Ala Trp Pro Lys Cys Ser Ser Cys Ser 
                325                 330                 335     


Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His Ile Leu Thr Phe Leu 
            340                 345                 350         


Ala Ala Glu Thr Gly Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
        355                 360                 365             


His His His His His His 
    370                 


<210>  4
<211>  938
<212>  PRT
<213>  artificial sequence

<220>
<223>  membrane-anchored fusion RPFB-Dhyb-Ag85B-TB10.4-ESAT6

<400>  4

Met Val Pro Gln Ala Leu Leu Leu Val Pro Leu Leu Gly Phe Ser Leu 
1               5                   10                  15      


Cys Phe Gly Lys Phe Pro Ile Asp Tyr Lys Asp Asp Asp Asp Lys Thr 
            20                  25                  30          


Val Asp Gly Thr Ala Met Arg Val Thr Thr Met Lys Ser Arg Val Ile 
        35                  40                  45              


Asp Ile Val Glu Glu Asn Gly Phe Ser Val Asp Asp Arg Asp Asp Leu 
    50                  55                  60                  


Tyr Pro Ala Ala Gly Val Gln Val His Asp Ala Asp Thr Ile Val Leu 
65                  70                  75                  80  


Arg Arg Ser Arg Pro Leu Gln Ile Ser Leu Asp Gly His Asp Ala Lys 
                85                  90                  95      


Gln Val Trp Thr Thr Ala Ser Thr Val Asp Glu Ala Leu Ala Gln Leu 
            100                 105                 110         


Ala Met Thr Asp Thr Ala Pro Ala Ala Ala Ser Arg Ala Ser Arg Val 
        115                 120                 125             


Pro Leu Ser Gly Met Ala Leu Pro Val Val Ser Ala Lys Thr Val Gln 
    130                 135                 140                 


Leu Asn Asp Gly Gly Leu Val Arg Thr Val His Leu Pro Ala Pro Asn 
145                 150                 155                 160 


Val Ala Gly Leu Leu Ser Ala Ala Gly Val Pro Leu Leu Gln Ser Asp 
                165                 170                 175     


His Val Val Pro Ala Ala Thr Ala Pro Ile Val Glu Gly Met Gln Ile 
            180                 185                 190         


Gln Val Thr Arg Asn Arg Ile Lys Lys Val Thr Glu Arg Leu Pro Leu 
        195                 200                 205             


Pro Pro Asn Ala Arg Arg Val Glu Asp Pro Glu Met Asn Met Ser Arg 
    210                 215                 220                 


Glu Val Val Glu Asp Pro Gly Val Pro Gly Thr Gln Asp Val Thr Phe 
225                 230                 235                 240 


Ala Val Ala Glu Val Asn Gly Val Glu Thr Gly Arg Leu Pro Val Ala 
                245                 250                 255     


Asn Val Val Val Thr Pro Ala His Glu Ala Val Val Arg Val Gly Thr 
            260                 265                 270         


Lys Pro Gly Thr Glu Val Pro Pro Val Ile Asp Gly Ser Ile Trp Asp 
        275                 280                 285             


Ala Ile Ala Gln Cys Lys Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly 
    290                 295                 300                 


Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Ala Trp Asp Ser 
305                 310                 315                 320 


Asn Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile 
                325                 330                 335     


Glu Val Ala Asp Asn Ile Met Lys Thr Ala Gly Pro Gly Ala Trp Pro 
            340                 345                 350         


Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr 
        355                 360                 365             


His Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly Ala Phe Ser Arg 
    370                 375                 380                 


Pro Gly Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly 
385                 390                 395                 400 


Arg Asp Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala 
                405                 410                 415     


Val Tyr Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp 
            420                 425                 430         


Asp Ile Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser 
        435                 440                 445             


Ile Val Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr 
    450                 455                 460                 


Ser Pro Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr 
465                 470                 475                 480 


Phe Leu Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val 
                485                 490                 495     


Lys Pro Thr Gly Ser Ala Ala Ile Gly Leu Ser Met Ala Gly Ser Ser 
            500                 505                 510         


Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe Ile Tyr Ala Gly 
        515                 520                 525             


Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met Gly Pro Ser Leu 
    530                 535                 540                 


Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys Ala Ala Asp Met 
545                 550                 555                 560 


Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn Asp Pro Thr Gln 
                565                 570                 575     


Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu Trp Val Tyr Cys 
            580                 585                 590         


Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn Ile Pro Ala Glu 
        595                 600                 605             


Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu Lys Phe Gln Asp Ala 
    610                 615                 620                 


Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn Phe Pro Pro Asn 
625                 630                 635                 640 


Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu Asn Ala Met Lys 
                645                 650                 655     


Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Ser Gln Ile Met Tyr Asn 
            660                 665                 670         


Tyr Pro Ala Met Leu Gly His Ala Gly Asp Met Ala Gly Tyr Ala Gly 
        675                 680                 685             


Thr Leu Gln Ser Leu Gly Ala Glu Ile Ala Val Glu Gln Ala Ala Leu 
    690                 695                 700                 


Gln Ser Ala Trp Gln Gly Asp Thr Gly Ile Thr Tyr Gln Ala Trp Gln 
705                 710                 715                 720 


Ala Gln Trp Asn Gln Ala Met Glu Asp Leu Val Arg Ala Tyr His Ala 
                725                 730                 735     


Met Ser Ser Thr His Glu Ala Asn Thr Met Ala Met Met Ala Arg Asp 
            740                 745                 750         


Thr Ala Glu Ala Ala Lys Trp Gly Gly Thr Glu Gln Gln Trp Asn Phe 
        755                 760                 765             


Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser 
    770                 775                 780                 


Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala 
785                 790                 795                 800 


Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln 
                805                 810                 815     


Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu 
            820                 825                 830         


Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly 
        835                 840                 845             


Asn Val Thr Gly Met Phe Ala Glu Gln Lys Leu Ile Ser Glu Glu Asp 
    850                 855                 860                 


Leu Ser Tyr Val Leu Leu Ser Ala Gly Thr Leu Ile Ala Leu Met Leu 
865                 870                 875                 880 


Ile Ile Phe Leu Ile Thr Cys Cys Lys Arg Val Asp Arg Pro Glu Ser 
                885                 890                 895     


Thr Gln Arg Ser Leu Arg Gly Thr Gly Arg Asn Val Ser Val Thr Ser 
            900                 905                 910         


Gln Ser Gly Lys Phe Ile Ser Ser Trp Glu Ser His Lys Ser Gly Gly 
        915                 920                 925             


Glu Thr Arg Leu His His His His His His 
    930                 935             


<210>  5
<211>  849
<212>  PRT
<213>  artificial sequence

<220>
<223>  cytoplasmic fusion RpfB-Dhyb-Ag85B-TB10.4-ESAT6

<400>  5

Met Asp Tyr Lys Asp Asp Asp Asp Lys Thr Val Asp Gly Thr Ala Met 
1               5                   10                  15      


Arg Val Thr Thr Met Lys Ser Arg Val Ile Asp Ile Val Glu Glu Asn 
            20                  25                  30          


Gly Phe Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro Ala Ala Gly Val 
        35                  40                  45              


Gln Val His Asp Ala Asp Thr Ile Val Leu Arg Arg Ser Arg Pro Leu 
    50                  55                  60                  


Gln Ile Ser Leu Asp Gly His Asp Ala Lys Gln Val Trp Thr Thr Ala 
65                  70                  75                  80  


Ser Thr Val Asp Glu Ala Leu Ala Gln Leu Ala Met Thr Asp Thr Ala 
                85                  90                  95      


Pro Ala Ala Ala Ser Arg Ala Ser Arg Val Pro Leu Ser Gly Met Ala 
            100                 105                 110         


Leu Pro Val Val Ser Ala Lys Thr Val Gln Leu Asn Asp Gly Gly Leu 
        115                 120                 125             


Val Arg Thr Val His Leu Pro Ala Pro Asn Val Ala Gly Leu Leu Ser 
    130                 135                 140                 


Ala Ala Gly Val Pro Leu Leu Gln Ser Asp His Val Val Pro Ala Ala 
145                 150                 155                 160 


Thr Ala Pro Ile Val Glu Gly Met Gln Ile Gln Val Thr Arg Asn Arg 
                165                 170                 175     


Ile Lys Lys Val Thr Glu Arg Leu Pro Leu Pro Pro Asn Ala Arg Arg 
            180                 185                 190         


Val Glu Asp Pro Glu Met Asn Met Ser Arg Glu Val Val Glu Asp Pro 
        195                 200                 205             


Gly Val Pro Gly Thr Gln Asp Val Thr Phe Ala Val Ala Glu Val Asn 
    210                 215                 220                 


Gly Val Glu Thr Gly Arg Leu Pro Val Ala Asn Val Val Val Thr Pro 
225                 230                 235                 240 


Ala His Glu Ala Val Val Arg Val Gly Thr Lys Pro Gly Thr Glu Val 
                245                 250                 255     


Pro Pro Val Ile Asp Gly Ser Ile Trp Asp Ala Ile Ala Gln Cys Lys 
            260                 265                 270         


Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu Tyr Gly Gly 
        275                 280                 285             


Leu Gln Ile Ser Gln Ala Ala Trp Asp Ser Asn Gly Gly Val Gly Ser 
    290                 295                 300                 


Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu Val Ala Asp Asn Ile 
305                 310                 315                 320 


Met Lys Thr Ala Gly Pro Gly Ala Trp Pro Lys Cys Ser Ser Cys Ser 
                325                 330                 335     


Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His Ile Leu Thr Phe Leu 
            340                 345                 350         


Ala Ala Glu Thr Gly Gly Ala Phe Ser Arg Pro Gly Leu Pro Val Glu 
        355                 360                 365             


Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp Ile Lys Val Gln 
    370                 375                 380                 


Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr Leu Leu Asp Gly 
385                 390                 395                 400 


Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile Asn Thr Pro Ala 
                405                 410                 415     


Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val Met Pro Val Gly 
            420                 425                 430         


Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro Ala Cys Gly Lys 
        435                 440                 445             


Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu 
    450                 455                 460                 


Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro Thr Gly Ser Ala 
465                 470                 475                 480 


Ala Ile Gly Leu Ser Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala 
                485                 490                 495     


Tyr His Pro Gln Gln Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu 
            500                 505                 510         


Asp Pro Ser Gln Gly Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly 
        515                 520                 525             


Asp Ala Gly Gly Tyr Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp 
    530                 535                 540                 


Pro Ala Trp Glu Arg Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val 
545                 550                 555                 560 


Ala Asn Asn Thr Arg Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn 
                565                 570                 575     


Glu Leu Gly Gly Ala Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val 
            580                 585                 590         


Arg Ser Ser Asn Leu Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly 
        595                 600                 605             


His Asn Ala Val Phe Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu 
    610                 615                 620                 


Tyr Trp Gly Ala Gln Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser 
625                 630                 635                 640 


Leu Gly Ala Gly Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met Leu Gly 
                645                 650                 655     


His Ala Gly Asp Met Ala Gly Tyr Ala Gly Thr Leu Gln Ser Leu Gly 
            660                 665                 670         


Ala Glu Ile Ala Val Glu Gln Ala Ala Leu Gln Ser Ala Trp Gln Gly 
        675                 680                 685             


Asp Thr Gly Ile Thr Tyr Gln Ala Trp Gln Ala Gln Trp Asn Gln Ala 
    690                 695                 700                 


Met Glu Asp Leu Val Arg Ala Tyr His Ala Met Ser Ser Thr His Glu 
705                 710                 715                 720 


Ala Asn Thr Met Ala Met Met Ala Arg Asp Thr Ala Glu Ala Ala Lys 
                725                 730                 735     


Trp Gly Gly Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala 
            740                 745                 750         


Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp 
        755                 760                 765             


Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser 
    770                 775                 780                 


Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala 
785                 790                 795                 800 


Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu 
                805                 810                 815     


Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe 
            820                 825                 830         


Ala Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu His His His His His 
        835                 840                 845             


His 
    


<210>  6
<211>  973
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion Ag85B-Rv2626-RPFB-Dhyb-Rv1733

<400>  6

Met Val Pro Gln Ala Leu Leu Phe Val Pro Leu Leu Val Phe Pro Leu 
1               5                   10                  15      


Cys Phe Gly Lys Phe Pro Ile Asp Tyr Lys Asp Asp Asp Asp Lys Ala 
            20                  25                  30          


Phe Ser Arg Pro Gly Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro 
        35                  40                  45              


Ser Met Gly Arg Asp Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn 
    50                  55                  60                  


Ser Pro Ala Val Tyr Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr 
65                  70                  75                  80  


Asn Gly Trp Asp Ile Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser 
                85                  90                  95      


Gly Leu Ser Ile Val Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser 
            100                 105                 110         


Asp Trp Tyr Ser Pro Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys 
        115                 120                 125             


Trp Glu Thr Phe Leu Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn 
    130                 135                 140                 


Arg Ala Val Lys Pro Thr Gly Ser Ala Ala Ile Gly Leu Ser Met Ala 
145                 150                 155                 160 


Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe Ile 
                165                 170                 175     


Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met Gly 
            180                 185                 190         


Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys Ala 
        195                 200                 205             


Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn Asp 
    210                 215                 220                 


Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu Trp 
225                 230                 235                 240 


Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn Ile 
                245                 250                 255     


Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu Lys Phe 
            260                 265                 270         


Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn Phe 
        275                 280                 285             


Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu Asn 
    290                 295                 300                 


Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Thr Thr Ala 
305                 310                 315                 320 


Arg Asp Ile Met Asn Ala Gly Val Thr Cys Val Gly Glu His Glu Thr 
                325                 330                 335     


Leu Thr Ala Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly Ala Leu 
            340                 345                 350         


Pro Ile Cys Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr Asp Arg 
        355                 360                 365             


Asp Ile Val Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn Thr Ala 
    370                 375                 380                 


Thr Ala Gly Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp Ala Asn 
385                 390                 395                 400 


Ala Ser Ile Gln Glu Met Leu Asn Val Met Glu Glu His Gln Val Arg 
                405                 410                 415     


Arg Val Pro Val Ile Ser Glu His Arg Leu Val Gly Ile Val Thr Glu 
            420                 425                 430         


Ala Asp Ile Ala Arg His Leu Pro Glu His Ala Ile Val Gln Phe Val 
        435                 440                 445             


Lys Ala Ile Cys Ser Pro Met Ala Leu Ala Ser Thr Val Asp Gly Thr 
    450                 455                 460                 


Ala Met Arg Val Thr Thr Met Lys Ser Arg Val Ile Asp Ile Val Glu 
465                 470                 475                 480 


Glu Asn Gly Phe Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro Ala Ala 
                485                 490                 495     


Gly Val Gln Val His Asp Ala Asp Thr Ile Val Leu Arg Arg Ser Arg 
            500                 505                 510         


Pro Leu Gln Ile Ser Leu Asp Gly His Asp Ala Lys Gln Val Trp Thr 
        515                 520                 525             


Thr Ala Ser Thr Val Asp Glu Ala Leu Ala Gln Leu Ala Met Thr Asp 
    530                 535                 540                 


Thr Ala Pro Ala Ala Ala Ser Arg Ala Ser Arg Val Pro Leu Ser Gly 
545                 550                 555                 560 


Met Ala Leu Pro Val Val Ser Ala Lys Thr Val Gln Leu Asn Asp Gly 
                565                 570                 575     


Gly Leu Val Arg Thr Val His Leu Pro Ala Pro Asn Val Ala Gly Leu 
            580                 585                 590         


Leu Ser Ala Ala Gly Val Pro Leu Leu Gln Ser Asp His Val Val Pro 
        595                 600                 605             


Ala Ala Thr Ala Pro Ile Val Glu Gly Met Gln Ile Gln Val Thr Arg 
    610                 615                 620                 


Asn Arg Ile Lys Lys Val Thr Glu Arg Leu Pro Leu Pro Pro Asn Ala 
625                 630                 635                 640 


Arg Arg Val Glu Asp Pro Glu Met Asn Met Ser Arg Glu Val Val Glu 
                645                 650                 655     


Asp Pro Gly Val Pro Gly Thr Gln Asp Val Thr Phe Ala Val Ala Glu 
            660                 665                 670         


Val Asn Gly Val Glu Thr Gly Arg Leu Pro Val Ala Asn Val Val Val 
        675                 680                 685             


Thr Pro Ala His Glu Ala Val Val Arg Val Gly Thr Lys Pro Gly Thr 
    690                 695                 700                 


Glu Val Pro Pro Val Ile Asp Gly Ser Ile Trp Asp Ala Ile Ala Gln 
705                 710                 715                 720 


Cys Lys Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu Tyr 
                725                 730                 735     


Gly Gly Leu Gln Ile Ser Gln Ala Ala Trp Asp Ser Asn Gly Gly Val 
            740                 745                 750         


Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu Val Ala Asp 
        755                 760                 765             


Asn Ile Met Lys Thr Ala Gly Pro Gly Ala Trp Pro Lys Cys Ser Ser 
    770                 775                 780                 


Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His Ile Leu Thr 
785                 790                 795                 800 


Phe Leu Ala Ala Glu Thr Gly Gly Ala Gly Thr Ala Val Gln Asp Ser 
                805                 810                 815     


Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro Ala Thr 
            820                 825                 830         


Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr Thr Ala 
        835                 840                 845             


Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg Trp Val 
    850                 855                 860                 


Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro Gly Thr 
865                 870                 875                 880 


Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly Gln Leu 
                885                 890                 895     


Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Ala Ala Leu 
            900                 905                 910         


Ala Ala Leu Gly Leu Trp Leu Ser Val Ala Ala Val Ala Gly Ala Leu 
        915                 920                 925             


Leu Ala Leu Thr Arg Ala Ile Leu Ile Arg Val Arg Asn Ala Ser Trp 
    930                 935                 940                 


Gln His Asp Ile Asp Ser Leu Phe Cys Thr Gln Arg Glu Gln Lys Leu 
945                 950                 955                 960 


Ile Ser Glu Glu Asp Leu Ser His His His His His His 
                965                 970             


<210>  7
<211>  1392
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence encoding membrane anchored RPFB-Dhyb protein

<400>  7
atggtaccac aagcgctgtt acttgtccca ctgcttggtt tctctttatg ttttggaaaa       60

ttcccaatag attacaagga tgacgacgat aagaccgtgg acggcaccgc catgagagtg      120

accaccatga agtccagagt gatcgacatc gtggaagaga acggcttcag cgtggacgac      180

cgggacgacc tgtatcctgc tgctggagtg caggtccacg acgccgatac aatcgtgctg      240

cggagaagca gacccctgca gatcagcctg gatggccacg acgccaagca ggtctggacc      300

acagccagca cagtggatga agccctggcc cagctggcca tgaccgatac agctccagcc      360

gccgctagca gagctagcag agtgcctctg tctggcatgg ccctgcctgt ggtgtctgcc      420

aagaccgtgc agctgaacga tggcggcctc gtgcggacag tgcatctgcc tgctcctaat      480

gtggccggcc tgctgtctgc agcaggcgtg ccactgctgc agagcgatca tgtggtgcct      540

gccgccacag cccctatcgt ggaaggcatg cagatccagg tcacacggaa ccggatcaag      600

aaagtgaccg agcggctgcc cctgcctccc aacgctagaa gagtggaaga tcccgagatg      660

aacatgagca gagaggtggt cgaggaccct ggcgtgccag gcacacagga tgtgacattc      720

gccgtggccg aagtgaacgg cgtggaaacc ggcagactgc ccgtggccaa tgtggtggtc      780

acaccagccc atgaggccgt cgtcagagtg ggcacaaagc ctggcacaga ggtgccaccc      840

gtgatcgacg gcagcatctg ggatgccatt gcccagtgca agagcggcgg aaactgggcc      900

gccaataccg gcaatggcct ctatggcggc ctgcagatct ctcaggccgc ctgggattct      960

aatggcggcg tgggatctcc tgccgctgcc tctccacagc agcagatcga ggtggccgac     1020

aacatcatga agacagccgg acctggcgcc tggcccaagt gtagcagttg ttctcagggc     1080

gacgcccctc tgggcagcct gacacacatc ctgacatttc tggccgccga gacaggcgga     1140

gaacaaaaac tcatctcaga agaggatctg agctatgttc ttctctctgc tggaacttta     1200

atagctttaa tgttaataat attcttaata acgtgctgta aaagggtaga ccgtccagag     1260

tcaactcagc gcagccttag gggtactggg agaaatgttt ccgtgacatc acagagtgga     1320

aaatttatct cgtcttggga atctcataag agtggaggcg aaacacgtct tcatcatcat     1380

catcatcatt ga                                                         1392


<210>  8
<211>  1125
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence encoding cytoplasmic RPFB-Dhyb protein

<400>  8
atggattaca aggatgacga cgataagacc gtggacggca ccgccatgag agtgaccacc       60

atgaagtcca gagtgatcga catcgtggaa gagaacggct tcagcgtgga cgaccgggac      120

gacctgtatc ctgctgctgg agtgcaggtc cacgacgccg atacaatcgt gctgcggaga      180

agcagacccc tgcagatcag cctggatggc cacgacgcca agcaggtctg gaccacagcc      240

agcacagtgg atgaagccct ggcccagctg gccatgaccg atacagctcc agccgccgct      300

agcagagcta gcagagtgcc tctgtctggc atggccctgc ctgtggtgtc tgccaagacc      360

gtgcagctga acgatggcgg cctcgtgcgg acagtgcatc tgcctgctcc taatgtggcc      420

ggcctgctgt ctgcagcagg cgtgccactg ctgcagagcg atcatgtggt gcctgccgcc      480

acagccccta tcgtggaagg catgcagatc caggtcacac ggaaccggat caagaaagtg      540

accgagcggc tgcccctgcc tcccaacgct agaagagtgg aagatcccga gatgaacatg      600

agcagagagg tggtcgagga ccctggcgtg ccaggcacac aggatgtgac attcgccgtg      660

gccgaagtga acggcgtgga aaccggcaga ctgcccgtgg ccaatgtggt ggtcacacca      720

gcccatgagg ccgtcgtcag agtgggcaca aagcctggca cagaggtgcc acccgtgatc      780

gacggcagca tctgggatgc cattgcccag tgcaagagcg gcggaaactg ggccgccaat      840

accggcaatg gcctctatgg cggcctgcag atctctcagg ccgcctggga ttctaatggc      900

ggcgtgggat ctcctgccgc tgcctctcca cagcagcaga tcgaggtggc cgacaacatc      960

atgaagacag ccggacctgg cgcctggccc aagtgtagca gttgttctca gggcgacgcc     1020

cctctgggca gcctgacaca catcctgaca tttctggccg ccgagacagg cggagaacaa     1080

aaactcatct cagaagagga tctgcatcat catcatcatc attga                     1125


<210>  9
<211>  2817
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence encoding membrane-anchored 
       RPFB-Dhyb-Ag85B-TB10.4-ESAT6 fusion

<400>  9
atggtaccac aagcgctgtt acttgtccca ctgcttggtt tctctttatg ttttggaaaa       60

ttcccaatag attacaagga tgacgacgat aagaccgtgg acggcaccgc catgagagtg      120

accaccatga agtccagagt gatcgacatc gtggaagaga acggcttcag cgtggacgac      180

cgggacgacc tgtatcctgc tgctggagtg caggtccacg acgccgatac aatcgtgctg      240

cggagaagca gacccctgca gatcagcctg gatggccacg acgccaagca ggtctggacc      300

acagccagca cagtggatga agccctggcc cagctggcca tgaccgatac agctccagcc      360

gccgctagca gagctagcag agtgcctctg tctggcatgg ccctgcctgt ggtgtctgcc      420

aagaccgtgc agctgaacga tggcggcctc gtgcggacag tgcatctgcc tgctcctaat      480

gtggccggcc tgctgtctgc agcaggcgtg ccactgctgc agagcgatca tgtggtgcct      540

gccgccacag cccctatcgt ggaaggcatg cagatccagg tcacacggaa ccggatcaag      600

aaagtgaccg agcggctgcc cctgcctccc aacgctagaa gagtggaaga tcccgagatg      660

aacatgagca gagaggtggt cgaggaccct ggcgtgccag gcacacagga tgtgacattc      720

gccgtggccg aagtgaacgg cgtggaaacc ggcagactgc ccgtggccaa tgtggtggtc      780

acaccagccc atgaggccgt cgtcagagtg ggcacaaagc ctggcacaga ggtgccaccc      840

gtgatcgacg gcagcatctg ggatgccatt gcccagtgca agagcggcgg aaactgggcc      900

gccaataccg gcaatggcct ctatggcggc ctgcagatct ctcaggccgc ctgggattct      960

aatggcggcg tgggatctcc tgccgctgcc tctccacagc agcagatcga ggtggccgac     1020

aacatcatga agacagccgg acctggcgcc tggcccaagt gtagcagttg ttctcagggc     1080

gacgcccctc tgggcagcct gacacacatc ctgacatttc tggccgccga gacaggcgga     1140

gccttctcta gacctggcct gcccgtggaa tacctgcagg tcccaagccc cagcatgggc     1200

cgggatatca aggtgcagtt tcagagcggc ggcaacaaca gccctgccgt gtatctgctg     1260

gatggcctga gagcccagga cgactacaac ggctgggaca tcaacacccc tgccttcgag     1320

tggtactacc agagcggcct gtccatcgtg atgcctgtgg gcggccagag cagcttctac     1380

agcgactggt acagccccgc ctgtggcaaa gccggctgcc agacctacaa gtgggagaca     1440

ttcctgacct ccgagctgcc ccagtggctg agcgccaata gagccgtgaa gcctacaggc     1500

tctgccgcca tcggactgag catggccgga agctctgcca tgatcctggc cgcctatcac     1560

cctcagcagt tcatctacgc cggcagcctg tctgccctgc tggacccttc tcagggcatg     1620

ggcccttctc tgatcggact ggctatgggc gacgctggcg gatacaaggc cgccgatatg     1680

tggggcccta gcagcgatcc tgcctgggag agaaacgacc ccacccagca gatccccaag     1740

ctggtggcca acaacacccg gctgtgggtg tactgcggca acggcacccc taatgaactg     1800

ggcggagcca atatccccgc cgagttcctg gaaaacttcg tgcggagcag caacctgaag     1860

ttccaggatg cctacaacgc cgctggcggc cacaacgccg tgttcaactt ccctcccaat     1920

ggcacccaca gctgggagta ctggggagcc cagctgaacg ccatgaaggg cgatctgcag     1980

tcctctctgg gagccggcag ccagatcatg tacaactacc ccgccatgct gggccacgcc     2040

ggcgatatgg ctggatatgc cggcacactg cagagcctgg gtgccgagat tgccgtggaa     2100

caggctgccc tccagtctgc ctggcagggc gataccggca tcacatacca ggcttggcag     2160

gcccagtgga accaggccat ggaagatctc gtgcgggcct accacgccat gagcagcaca     2220

cacgaggcca acaccatggc catgatggcc cgggatacag ccgaggccgc taagtgggga     2280

ggaaccgagc agcagtggaa cttcgccgga attgaggccg ctgccagcgc catccagggc     2340

aacgtgacat ccatccacag cctgctggac gagggcaagc agagcctgac aaaactggct     2400

gctgcctggg gcggctctgg ctctgaagct tatcagggcg tgcagcagaa gtgggacgcc     2460

accgccaccg agctgaacaa cgccctgcag aacctggccc ggacaatctc tgaagccgga     2520

caggccatgg ccagcaccga gggcaatgtg accggcatgt ttgccgaaca aaaactcatc     2580

tcagaagagg atctgagcta tgttcttctc tctgctggaa ctttaatagc tttaatgtta     2640

ataatattct taataacgtg ctgtaaaagg gtagaccgtc cagagtcaac tcagcgcagc     2700

cttaggggta ctgggagaaa tgtttccgtg acatcacaga gtggaaaatt tatctcgtct     2760

tgggaatctc ataagagtgg aggcgaaaca cgtcttcatc atcatcatca tcattga        2817


<210>  10
<211>  2550
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence encoding cytoplasmic 
       RPFB-Dhyb-Ag85B-TB10.4-ESAT6 fusion

<400>  10
atggattaca aggatgacga cgataagacc gtggacggca ccgccatgag agtgaccacc       60

atgaagtcca gagtgatcga catcgtggaa gagaacggct tcagcgtgga cgaccgggac      120

gacctgtatc ctgctgctgg agtgcaggtc cacgacgccg atacaatcgt gctgcggaga      180

agcagacccc tgcagatcag cctggatggc cacgacgcca agcaggtctg gaccacagcc      240

agcacagtgg atgaagccct ggcccagctg gccatgaccg atacagctcc agccgccgct      300

agcagagcta gcagagtgcc tctgtctggc atggccctgc ctgtggtgtc tgccaagacc      360

gtgcagctga acgatggcgg cctcgtgcgg acagtgcatc tgcctgctcc taatgtggcc      420

ggcctgctgt ctgcagcagg cgtgccactg ctgcagagcg atcatgtggt gcctgccgcc      480

acagccccta tcgtggaagg catgcagatc caggtcacac ggaaccggat caagaaagtg      540

accgagcggc tgcccctgcc tcccaacgct agaagagtgg aagatcccga gatgaacatg      600

agcagagagg tggtcgagga ccctggcgtg ccaggcacac aggatgtgac attcgccgtg      660

gccgaagtga acggcgtgga aaccggcaga ctgcccgtgg ccaatgtggt ggtcacacca      720

gcccatgagg ccgtcgtcag agtgggcaca aagcctggca cagaggtgcc acccgtgatc      780

gacggcagca tctgggatgc cattgcccag tgcaagagcg gcggaaactg ggccgccaat      840

accggcaatg gcctctatgg cggcctgcag atctctcagg ccgcctggga ttctaatggc      900

ggcgtgggat ctcctgccgc tgcctctcca cagcagcaga tcgaggtggc cgacaacatc      960

atgaagacag ccggacctgg cgcctggccc aagtgtagca gttgttctca gggcgacgcc     1020

cctctgggca gcctgacaca catcctgaca tttctggccg ccgagacagg cggagccttc     1080

tctagacctg gcctgcccgt ggaatacctg caggtcccaa gccccagcat gggccgggat     1140

atcaaggtgc agtttcagag cggcggcaac aacagccctg ccgtgtatct gctggatggc     1200

ctgagagccc aggacgacta caacggctgg gacatcaaca cccctgcctt cgagtggtac     1260

taccagagcg gcctgtccat cgtgatgcct gtgggcggcc agagcagctt ctacagcgac     1320

tggtacagcc ccgcctgtgg caaagccggc tgccagacct acaagtggga gacattcctg     1380

acctccgagc tgccccagtg gctgagcgcc aatagagccg tgaagcctac aggctctgcc     1440

gccatcggac tgagcatggc cggaagctct gccatgatcc tggccgccta tcaccctcag     1500

cagttcatct acgccggcag cctgtctgcc ctgctggacc cttctcaggg catgggccct     1560

tctctgatcg gactggctat gggcgacgct ggcggataca aggccgccga tatgtggggc     1620

cctagcagcg atcctgcctg ggagagaaac gaccccaccc agcagatccc caagctggtg     1680

gccaacaaca cccggctgtg ggtgtactgc ggcaacggca cccctaatga actgggcgga     1740

gccaatatcc ccgccgagtt cctggaaaac ttcgtgcgga gcagcaacct gaagttccag     1800

gatgcctaca acgccgctgg cggccacaac gccgtgttca acttccctcc caatggcacc     1860

cacagctggg agtactgggg agcccagctg aacgccatga agggcgatct gcagtcctct     1920

ctgggagccg gcagccagat catgtacaac taccccgcca tgctgggcca cgccggcgat     1980

atggctggat atgccggcac actgcagagc ctgggtgccg agattgccgt ggaacaggct     2040

gccctccagt ctgcctggca gggcgatacc ggcatcacat accaggcttg gcaggcccag     2100

tggaaccagg ccatggaaga tctcgtgcgg gcctaccacg ccatgagcag cacacacgag     2160

gccaacacca tggccatgat ggcccgggat acagccgagg ccgctaagtg gggaggaacc     2220

gagcagcagt ggaacttcgc cggaattgag gccgctgcca gcgccatcca gggcaacgtg     2280

acatccatcc acagcctgct ggacgagggc aagcagagcc tgacaaaact ggctgctgcc     2340

tggggcggct ctggctctga agcttatcag ggcgtgcagc agaagtggga cgccaccgcc     2400

accgagctga acaacgccct gcagaacctg gcccggacaa tctctgaagc cggacaggcc     2460

atggccagca ccgagggcaa tgtgaccggc atgtttgccg aacaaaaact catctcagaa     2520

gaggatctgc atcatcatca tcatcattga                                      2550


<210>  11
<211>  2922
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence encoding Ag85B-Rv2626-RPFB-Dhyb-Rv1733 fusion

<400>  11
atggttcctc aggctctcct gtttgtaccc cttctggttt ttccattgtg ttttgggaaa       60

ttccctattg attacaagga tgacgacgat aaggccttct ctagacctgg cctgcccgtg      120

gaatacctgc aggtcccaag ccccagcatg ggccgggata tcaaggtgca gtttcagagc      180

ggcggcaaca acagccctgc cgtgtatctg ctggatggcc tgagagccca ggacgactac      240

aacggctggg acatcaacac ccctgccttc gagtggtact accagagcgg cctgtccatc      300

gtgatgcctg tgggcggcca gagcagcttc tacagcgact ggtacagccc cgcctgtggc      360

aaagccggct gccagaccta caagtgggag acattcctga cctccgagct gccccagtgg      420

ctgagcgcca atagagccgt gaagcctaca ggctctgccg ccatcggact gagcatggcc      480

ggaagctctg ccatgatcct ggccgcctat caccctcagc agttcatcta cgccggcagc      540

ctgtctgccc tgctggaccc ttctcagggc atgggccctt ctctgatcgg actggctatg      600

ggcgacgctg gcggatacaa ggccgccgat atgtggggcc ctagcagcga tcctgcctgg      660

gagagaaacg accccaccca gcagatcccc aagctggtgg ccaacaacac ccggctgtgg      720

gtgtactgcg gcaacggcac ccctaatgaa ctgggcggag ccaatatccc cgccgagttc      780

ctggaaaact tcgtgcggag cagcaacctg aagttccagg atgcctacaa cgccgctggc      840

ggccacaacg ccgtgttcaa cttccctccc aatggcaccc acagctggga gtactgggga      900

gcccagctga acgccatgaa gggcgatctg cagtcctctc tgggagccgg cacaaccgcc      960

agagacatca tgaacgccgg cgtgacctgt gtgggcgagc acgagacact gacagccgcc     1020

gctcagtaca tgagagagca cgacatcggc gccctgccca tctgcggcga cgatgataga     1080

ctgcacggca tgctgaccga ccgggacatc gtgatcaagg gcctggctgc tggcctggac     1140

cccaatactg ctacagctgg cgagctggca agagacagca tctactacgt ggacgccaac     1200

gccagcatcc aggaaatgct gaacgtgatg gaagaacacc aggtccgacg ggtgcccgtg     1260

atcagcgaac acagactcgt gggcatcgtg accgaggccg atatcgccag acatctgccc     1320

gagcacgcca tcgtgcagtt cgtgaaggcc atctgcagcc ccatggccct ggcctctacc     1380

gtggacggca ccgccatgag agtgaccacc atgaagtcca gagtgatcga catcgtggaa     1440

gagaacggct tcagcgtgga cgaccgggac gacctgtatc ctgctgctgg agtgcaggtc     1500

cacgacgccg atacaatcgt gctgcggaga agcagacccc tgcagatcag cctggatggc     1560

cacgacgcca agcaggtctg gaccacagcc agcacagtgg atgaagccct ggcccagctg     1620

gccatgaccg atacagctcc agccgccgct agcagagcta gcagagtgcc tctgtctggc     1680

atggccctgc ctgtggtgtc tgccaagacc gtgcagctga acgatggcgg cctcgtgcgg     1740

acagtgcatc tgcctgctcc taatgtggcc ggcctgctgt ctgcagcagg cgtgccactg     1800

ctgcagagcg atcatgtggt gcctgccgcc acagccccta tcgtggaagg catgcagatc     1860

caggtcacac ggaaccggat caagaaagtg accgagcggc tgcccctgcc tcccaacgct     1920

agaagagtgg aagatcccga gatgaacatg agcagagagg tggtcgagga ccctggcgtg     1980

ccaggcacac aggatgtgac attcgccgtg gccgaagtga acggcgtgga aaccggcaga     2040

ctgcccgtgg ccaatgtggt ggtcacacca gcccatgagg ccgtcgtcag agtgggcaca     2100

aagcctggca cagaggtgcc acccgtgatc gacggcagca tctgggatgc cattgcccag     2160

tgcaagagcg gcggaaactg ggccgccaat accggcaatg gcctctatgg cggcctgcag     2220

atctctcagg ccgcctggga ttctaatggc ggcgtgggat ctcctgccgc tgcctctcca     2280

cagcagcaga tcgaggtggc cgacaacatc atgaagacag ccggacctgg cgcctggccc     2340

aagtgtagca gttgttctca gggcgacgcc cctctgggca gcctgacaca catcctgaca     2400

tttctggccg ccgagacagg cggagctgga acagccgtgc aggatagccg gtcccacgtg     2460

tacgctcacc aggcccagac aagacaccct gccacagcca ccgtgatcga ccacgagggc     2520

gtgatcgaca gcaacaccac cgccacatct gccccacccc ggaccaagat cacagtgcct     2580

gctagatggg tggtcaacgg catcgagcgg agcggcgaag tgaatgccaa gcccggcacc     2640

aagagcggcg acagagtggg aatctgggtg gactctgccg gccagctggt ggatgaacct     2700

gcccctcctg ccagagccat tgccgatgct gctctggctg cactgggcct gtggctgtct     2760

gtggcagctg tggctggcgc actgctggct ctgacaagag ccatcctgat cagagtgcgg     2820

aacgccagtt ggcagcacga tatcgacagc ctgttctgca cccagcggga acaaaaactc     2880

atctcagaag aggatctgag ccatcatcat catcatcatt ga                        2922


<210>  12
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  Flag tag peptide

<400>  12

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  13
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  C-MYC tag peptide

<400>  13

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  14
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  Histidine tag peptide

<400>  14

His His His His His His 
1               5       


<210>  15
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  15
cgcggccgca ccatggatta caaggatgac gacg                                   34


<210>  16
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  16
cgtcgtcatc cttgtaatcc atggtgcggc cgcg                                   34


<210>  17
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  17
catctcagaa gaggatctgc atcatcatca tcatcattg                              39


<210>  18
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  18
caatgatgat gatgatgatg cagatcctct tctgagatg                              39


