                         SEQUENCE LISTING

<110>  Novo Nordisk A/S
 
<120>  USE OF LONG-ACTING GLP-1 PEPTIDES

<130>  8545.204-WO

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  31
<212>  PRT
<213>  Homo sapiens

<400>  1

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly 
            20                  25                  30      


<210>  2
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GLP-1 agonist


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  This residue is H-His

<220>
<221>  MISC_FEATURE
<222>  (39)..(39)
<223>  This residue is Ser-NH2

<400>  2

His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu 
1               5                   10                  15      


Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  3
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GLP-1 agonist


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Aib

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Aib

<400>  3

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  30  


<210>  4
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GLP-1 agonist


<220>
<221>  MISC_FEATURE
<222>  (60)..(60)
<223>  genetically fused to human albumin

<400>  4

His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg His Gly 
            20                  25                  30          


Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
        35                  40                  45              


Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg 
    50                  55                  60  


<210>  5
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GLP-1 agonist


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  L-histidine, D-histidine, desamino-histidine, 2-amino-histidine, 
       beta-hydroxy-histidine, homohistidine, Nalpha-acetyl-histidine, 
       alpha-fluoromethyl-histidine, alpha-methyl-histidine, 
       3-pyridylalanine, 2-pyridylalanine or 4-pyridylalanine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Ala, Gly, Val, Leu, Ile, Lys, Aib, (1-aminocyclopropyl)carboxylic
       acid, (1- aminocyclobutyl) carboxylic acid, 
       (1-aminocyclopentyl)carboxylic acid, 
       (1-aminocyclohexyl)carboxylic acid, 
       

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Val or Leu

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Ser, Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Tyr or Gln

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Leu or Met

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Gly, Glu or Aib

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Gln, Glu, Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Ala or Val

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Lys, Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Glu or Leu

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Ala, Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Val or Lys

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Lys, Glu, Asn or Arg

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Gly or Aib

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Arg, Gly or Lys

<220>
<221>  MISC_FEATURE
<222>  (31)..(31)
<223>  Gly, Ala, Glu, Pro, Lys, amide or absent

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Lys, Ser, amide or absent

<220>
<221>  MISC_FEATURE
<222>  (33)..(33)
<223>  Ser, Lys, amide or absent, provided that this residue is absent 
       if any of the preceding residues are absent

<220>
<221>  MISC_FEATURE
<222>  (34)..(34)
<223>  Gly, amide or absent, provided that this residue is absent if any
       of the preceding residues are absent

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  Ala, amide or absent, provided that this residue is absent if any
       of the preceding residues are absent

<220>
<221>  MISC_FEATURE
<222>  (36)..(36)
<223>  Pro, amide or absent, provided that this residue is absent if any
       of the preceding residues are absent

<220>
<221>  MISC_FEATURE
<222>  (37)..(37)
<223>  Pro, amide or absent, provided that this residue is absent if any
       of the preceding residues are absent

<220>
<221>  MISC_FEATURE
<222>  (38)..(38)
<223>  Pro, amide or absent, provided that this residue is absent if any
       of the preceding residues are absent

<220>
<221>  MISC_FEATURE
<222>  (39)..(39)
<223>  Ser, amide or absent, provided that this residue is absent if any
       of the preceding residues are absent

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  amide or absent, provided that this residue is absent if any of 
       the preceding residues are absent

<400>  5

Xaa Xaa Glu Gly Thr Phe Thr Ser Asp Xaa Ser Xaa Xaa Xaa Glu Xaa 
1               5                   10                  15      


Xaa Ala Xaa Xaa Xaa Phe Ile Xaa Trp Leu Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40  


<210>  6
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GLP-1 agonist


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  L-histidine, D-histidine, desamino-histidine, 2-amino-histidine, 
       -hydroxy-histidine, homohistidine, Nalpha-acetyl-histidine, 
       alpha-fluoromethyl-histidine, alpha-methyl-histidine, 
       3-pyridylalanine, 2-pyridylalanine or 4-pyridylalanine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Ala, Gly, Val, Leu, lle, Lys, Aib, (1-aminocyclopropyl) 
       carboxylic acid, (1- aminocyclobutyl) carboxylic acid, 
       (1-aminocyclopentyl) carboxylic acid, (1-aminocyclohexyl) 
       carboxylic acid, (1-aminocycloheptyl)carboxylic acid, or 
       

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Ser, Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Gly, Glu or Aib

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Gln, Glu, Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Lys, Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Ala, Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Lys, Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Gly or Aib

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Arg or Lys

<220>
<221>  MISC_FEATURE
<222>  (31)..(31)
<223>  Gly, Ala, Glu or Lys

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Lys, amide or is absent

<400>  6

Xaa Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Xaa Tyr Leu Glu Xaa 
1               5                   10                  15      


Xaa Ala Ala Xaa Glu Phe Ile Xaa Trp Leu Val Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


