                         SEQUENCE LISTING

<110>  Thompson, Tracy
       Rehm, Bernd H A
       Herbert, Andrew B
       Saravolac, Edward G
       McDermott, Paul B
       Draper, Jenny L
 
<120>  COMPOSITIONS FOR SEPARATION METHODS

<130>  670011 JBM

<150>  US 61/661288
<151>  2012-06-18

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  743
<212>  PRT
<213>  Synthetic - SN-P1 fusion polypeptide constructed in laboratory

<400>  1

Met Ser Pro His Pro His Pro Arg His His His Thr Leu Glu Arg Thr 
1               5                   10                  15      


Gly Gly Gly Gly Gly Phe Glu Ala Gln His Asp Glu Ala Val Asp Asn 
            20                  25                  30          


Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu 
        35                  40                  45              


Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys 
    50                  55                  60                  


Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu 
65                  70                  75                  80  


Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln 
                85                  90                  95      


Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu 
            100                 105                 110         


Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser 
        115                 120                 125             


Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro 
    130                 135                 140                 


Lys Val Asp Ala Asn Ser Ser Pro Arg His Met Ala Thr Gly Lys Gly 
145                 150                 155                 160 


Ala Ala Ala Ser Thr Gln Glu Gly Lys Ser Gln Pro Phe Lys Val Thr 
                165                 170                 175     


Pro Gly Pro Phe Asp Pro Ala Thr Trp Leu Glu Trp Ser Arg Gln Trp 
            180                 185                 190         


Gln Gly Thr Glu Gly Asn Gly His Ala Ala Ala Ser Gly Ile Pro Gly 
        195                 200                 205             


Leu Asp Ala Leu Ala Gly Val Lys Ile Ala Pro Ala Gln Leu Gly Asp 
    210                 215                 220                 


Ile Gln Gln Arg Tyr Met Lys Asp Phe Ser Ala Leu Trp Gln Ala Met 
225                 230                 235                 240 


Ala Glu Gly Lys Ala Glu Ala Thr Gly Pro Leu His Asp Arg Arg Phe 
                245                 250                 255     


Ala Gly Asp Ala Trp Arg Thr Asn Leu Pro Tyr Arg Phe Ala Ala Ala 
            260                 265                 270         


Phe Tyr Leu Leu Asn Ala Arg Ala Leu Thr Glu Leu Ala Asp Ala Val 
        275                 280                 285             


Glu Ala Asp Ala Lys Thr Arg Gln Arg Ile Arg Phe Ala Ile Ser Gln 
    290                 295                 300                 


Trp Val Asp Ala Met Ser Pro Ala Asn Phe Leu Ala Thr Asn Pro Glu 
305                 310                 315                 320 


Ala Gln Arg Leu Leu Ile Glu Ser Gly Gly Glu Ser Leu Arg Ala Gly 
                325                 330                 335     


Val Arg Asn Met Met Glu Asp Leu Thr Arg Gly Lys Ile Ser Gln Thr 
            340                 345                 350         


Asp Glu Ser Ala Phe Glu Val Gly Arg Asn Val Ala Val Thr Glu Gly 
        355                 360                 365             


Ala Val Val Phe Glu Asn Glu Tyr Phe Gln Leu Leu Gln Tyr Lys Pro 
    370                 375                 380                 


Leu Thr Asp Lys Val His Ala Arg Pro Leu Leu Met Val Pro Pro Cys 
385                 390                 395                 400 


Ile Asn Lys Tyr Tyr Ile Leu Asp Leu Gln Pro Glu Ser Ser Leu Val 
                405                 410                 415     


Arg His Val Val Glu Gln Gly His Thr Val Phe Leu Val Ser Trp Arg 
            420                 425                 430         


Asn Pro Asp Ala Ser Met Ala Gly Ser Thr Trp Asp Asp Tyr Ile Glu 
        435                 440                 445             


His Ala Ala Ile Arg Ala Ile Glu Val Ala Arg Asp Ile Ser Gly Gln 
    450                 455                 460                 


Asp Lys Ile Asn Val Leu Gly Phe Cys Val Gly Gly Thr Ile Val Ser 
465                 470                 475                 480 


Thr Ala Leu Ala Val Leu Ala Ala Arg Gly Glu His Pro Ala Ala Ser 
                485                 490                 495     


Val Thr Leu Leu Thr Thr Leu Leu Asp Phe Ala Asp Thr Gly Ile Leu 
            500                 505                 510         


Asp Val Phe Val Asp Glu Gly His Val Gln Leu Arg Glu Ala Thr Leu 
        515                 520                 525             


Gly Gly Gly Ala Gly Ala Pro Cys Ala Leu Leu Arg Gly Leu Glu Leu 
    530                 535                 540                 


Ala Asn Thr Phe Ser Phe Leu Arg Pro Asn Asp Leu Val Trp Asn Tyr 
545                 550                 555                 560 


Val Val Asp Asn Tyr Leu Lys Gly Asn Thr Pro Val Pro Phe Asp Leu 
                565                 570                 575     


Leu Phe Trp Asn Gly Asp Ala Thr Asn Leu Pro Gly Pro Trp Tyr Cys 
            580                 585                 590         


Trp Tyr Leu Arg His Thr Tyr Leu Gln Asn Glu Leu Lys Val Pro Gly 
        595                 600                 605             


Lys Leu Thr Val Cys Gly Val Pro Val Asp Leu Ala Ser Ile Asp Val 
    610                 615                 620                 


Pro Thr Tyr Ile Tyr Gly Ser Arg Glu Asp His Ile Val Pro Trp Thr 
625                 630                 635                 640 


Ala Ala Tyr Ala Ser Thr Ala Leu Leu Ala Asn Lys Leu Arg Phe Val 
                645                 650                 655     


Leu Gly Ala Ser Gly His Ile Ala Gly Val Ile Asn Pro Pro Ala Lys 
            660                 665                 670         


Asn Lys Arg Ser His Trp Thr Asn Asp Ala Leu Pro Glu Ser Pro Gln 
        675                 680                 685             


Gln Trp Leu Ala Gly Ala Ile Glu His His Gly Ser Trp Trp Pro Asp 
    690                 695                 700                 


Trp Thr Ala Trp Leu Ala Gly Gln Ala Gly Ala Lys Arg Ala Ala Pro 
705                 710                 715                 720 


Ala Asn Tyr Gly Asn Ala Arg Tyr Arg Ala Ile Glu Pro Ala Pro Gly 
                725                 730                 735     


Arg Tyr Val Lys Ala Lys Ala 
            740             


<210>  2
<211>  2232
<212>  DNA
<213>  Synthetic - encodes SN-P1 fusion polypeptide constructed in laboratory

<400>  2
atgagcccgc atccgcatcc gcgccatcat cataccctcg agcgtacggg aggcggtgga       60

ggcttcgaag cgcaacacga tgaagccgta gacaacaaat tcaacaaaga acaacaaaac      120

gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc      180

caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta      240

aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca aaacgcgttc      300

tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt      360

ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat      420

gctcaggcgc cgaaagtaga cgcgaattcg agccctaggc atatggcgac cggcaaaggc      480

gcggcagctt ccacgcagga aggcaagtcc caaccattca aggtcacgcc ggggccattc      540

gatccagcca catggctgga atggtcccgc cagtggcagg gcactgaagg caacggccac      600

gcggccgcgt ccggcattcc gggcctggat gcgctggcag gcgtcaagat cgcgccggcg      660

cagctgggtg atatccagca gcgctacatg aaggacttct cagcgctgtg gcaggccatg      720

gccgagggca aggccgaggc caccggtccg ctgcacgacc ggcgcttcgc cggcgacgca      780

tggcgcacca acctcccata tcgcttcgct gccgcgttct acctgctcaa tgcgcgcgcc      840

ttgaccgagc tggccgatgc cgtcgaggcc gatgccaaga cccgccagcg catccgcttc      900

gcgatctcgc aatgggtcga tgcgatgtcg cccgccaact tccttgccac caatcccgag      960

gcgcagcgcc tgctgatcga gtcgggcggc gaatcgctgc gtgccggcgt gcgcaacatg     1020

atggaagacc tgacacgcgg caagatctcg cagaccgacg agagcgcgtt tgaggtcggc     1080

cgcaatgtcg cggtgaccga aggcgccgtg gtcttcgaga acgagtactt ccagctgttg     1140

cagtacaagc cgctgaccga caaggtgcac gcgcgcccgc tgctgatggt gccgccgtgc     1200

atcaacaagt actacatcct ggacctgcag ccggagagct cgctggtgcg ccatgtggtg     1260

gagcagggac atacggtgtt tctggtgtcg tggcgcaatc cggacgccag catggccggc     1320

agcacctggg acgactacat cgagcacgcg gccatccgcg ccatcgaagt cgcgcgcgac     1380

atcagcggcc aggacaagat caacgtgctc ggcttctgcg tgggcggcac cattgtctcg     1440

accgcgctgg cggtgctggc cgcgcgcggc gagcacccgg ccgccagcgt cacgctgctg     1500

accacgctgc tggactttgc cgacacgggc atcctcgacg tctttgtcga cgagggccat     1560

gtgcagttgc gcgaggccac gctgggcggc ggcgccggcg cgccgtgcgc gctgctgcgc     1620

ggccttgagc tggccaatac cttctcgttc ttgcgcccga acgacctggt gtggaactac     1680

gtggtcgaca actacctgaa gggcaacacg ccggtgccgt tcgacctgct gttctggaac     1740

ggcgacgcca ccaacctgcc ggggccgtgg tactgctggt acctgcgcca cacctacctg     1800

cagaacgagc tcaaggtacc gggcaagctg accgtgtgcg gcgtgccggt ggacctggcc     1860

agcatcgacg tgccgaccta tatctacggc tcgcgcgaag accatatcgt gccgtggacc     1920

gcggcctatg cctcgaccgc gctgctggcg aacaagctgc gcttcgtgct gggtgcgtcg     1980

ggccatatcg ccggtgtgat caacccgccg gccaagaaca agcgcagcca ctggactaac     2040

gatgcgctgc cggagtcgcc gcagcaatgg ctggccggcg ccatcgagca tcacggcagc     2100

tggtggccgg actggaccgc atggctggcc gggcaggccg gcgcgaaacg cgccgcgccc     2160

gccaactatg gcaatgcgcg ctatcgcgca atcgaacccg cgcctgggcg atacgtcaaa     2220

gccaaggcat ga                                                         2232


<210>  3
<211>  1053
<212>  PRT
<213>  Synthetic - SN-P3 fusion polypeptide constructed in laboratory

<400>  3

Met Ser Pro His Pro His Pro Arg His His His Thr Leu Glu Arg Thr 
1               5                   10                  15      


Gly Gly Gly Gly Gly Phe Glu Ala Gln His Asp Glu Ala Val Asp Asn 
            20                  25                  30          


Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu 
        35                  40                  45              


Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys 
    50                  55                  60                  


Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu 
65                  70                  75                  80  


Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln 
                85                  90                  95      


Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu 
            100                 105                 110         


Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser 
        115                 120                 125             


Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro 
    130                 135                 140                 


Lys Val Asp Ala Asn Ser Ser Ser Val Pro Arg Thr Gly Gly Gly Gly 
145                 150                 155                 160 


Gly Phe Glu Ala Gln His Asp Glu Ala Val Asp Asn Lys Phe Asn Lys 
                165                 170                 175     


Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn 
            180                 185                 190         


Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser 
        195                 200                 205             


Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
    210                 215                 220                 


Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe 
225                 230                 235                 240 


Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala 
                245                 250                 255     


Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu 
            260                 265                 270         


Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Ala 
        275                 280                 285             


Asn Ser Ser Pro Arg His Met Ala Thr Gly Lys Gly Ala Ala Ala Ser 
    290                 295                 300                 


Thr Gln Glu Gly Lys Ser Gln Pro Phe Lys Val Thr Pro Gly Pro Phe 
305                 310                 315                 320 


Asp Pro Ala Thr Trp Leu Glu Trp Ser Arg Gln Trp Gln Gly Thr Glu 
                325                 330                 335     


Gly Asn Gly His Ala Ala Ala Ser Gly Ile Pro Gly Leu Asp Ala Leu 
            340                 345                 350         


Ala Gly Val Lys Ile Ala Pro Ala Gln Leu Gly Asp Ile Gln Gln Arg 
        355                 360                 365             


Tyr Met Lys Asp Phe Ser Ala Leu Trp Gln Ala Met Ala Glu Gly Lys 
    370                 375                 380                 


Ala Glu Ala Thr Gly Pro Leu His Asp Arg Arg Phe Ala Gly Asp Ala 
385                 390                 395                 400 


Trp Arg Thr Asn Leu Pro Tyr Arg Phe Ala Ala Ala Phe Tyr Leu Leu 
                405                 410                 415     


Asn Ala Arg Ala Leu Thr Glu Leu Ala Asp Ala Val Glu Ala Asp Ala 
            420                 425                 430         


Lys Thr Arg Gln Arg Ile Arg Phe Ala Ile Ser Gln Trp Val Asp Ala 
        435                 440                 445             


Met Ser Pro Ala Asn Phe Leu Ala Thr Asn Pro Glu Ala Gln Arg Leu 
    450                 455                 460                 


Leu Ile Glu Ser Gly Gly Glu Ser Leu Arg Ala Gly Val Arg Asn Met 
465                 470                 475                 480 


Met Glu Asp Leu Thr Arg Gly Lys Ile Ser Gln Thr Asp Glu Ser Ala 
                485                 490                 495     


Phe Glu Val Gly Arg Asn Val Ala Val Thr Glu Gly Ala Val Val Phe 
            500                 505                 510         


Glu Asn Glu Tyr Phe Gln Leu Leu Gln Tyr Lys Pro Leu Thr Asp Lys 
        515                 520                 525             


Val His Ala Arg Pro Leu Leu Met Val Pro Pro Cys Ile Asn Lys Tyr 
    530                 535                 540                 


Tyr Ile Leu Asp Leu Gln Pro Glu Ser Ser Leu Val Arg His Val Val 
545                 550                 555                 560 


Glu Gln Gly His Thr Val Phe Leu Val Ser Trp Arg Asn Pro Asp Ala 
                565                 570                 575     


Ser Met Ala Gly Ser Thr Trp Asp Asp Tyr Ile Glu His Ala Ala Ile 
            580                 585                 590         


Arg Ala Ile Glu Val Ala Arg Asp Ile Ser Gly Gln Asp Lys Ile Asn 
        595                 600                 605             


Val Leu Gly Phe Cys Val Gly Gly Thr Ile Val Ser Thr Ala Leu Ala 
    610                 615                 620                 


Val Leu Ala Ala Arg Gly Glu His Pro Ala Ala Ser Val Thr Leu Leu 
625                 630                 635                 640 


Thr Thr Leu Leu Asp Phe Ala Asp Thr Gly Ile Leu Asp Val Phe Val 
                645                 650                 655     


Asp Glu Gly His Val Gln Leu Arg Glu Ala Thr Leu Gly Gly Gly Ala 
            660                 665                 670         


Gly Ala Pro Cys Ala Leu Leu Arg Gly Leu Glu Leu Ala Asn Thr Phe 
        675                 680                 685             


Ser Phe Leu Arg Pro Asn Asp Leu Val Trp Asn Tyr Val Val Asp Asn 
    690                 695                 700                 


Tyr Leu Lys Gly Asn Thr Pro Val Pro Phe Asp Leu Leu Phe Trp Asn 
705                 710                 715                 720 


Gly Asp Ala Thr Asn Leu Pro Gly Pro Trp Tyr Cys Trp Tyr Leu Arg 
                725                 730                 735     


His Thr Tyr Leu Gln Asn Glu Leu Lys Val Pro Gly Lys Leu Thr Val 
            740                 745                 750         


Cys Gly Val Pro Val Asp Leu Ala Ser Ile Asp Val Pro Thr Tyr Ile 
        755                 760                 765             


Tyr Gly Ser Arg Glu Asp His Ile Val Pro Trp Thr Ala Ala Tyr Ala 
    770                 775                 780                 


Ser Thr Ala Leu Leu Ala Asn Lys Leu Arg Phe Val Leu Gly Ala Ser 
785                 790                 795                 800 


Gly His Ile Ala Gly Val Ile Asn Pro Pro Ala Lys Asn Lys Arg Ser 
                805                 810                 815     


His Trp Thr Asn Asp Ala Leu Pro Glu Ser Pro Gln Gln Trp Leu Ala 
            820                 825                 830         


Gly Ala Ile Glu His His Gly Ser Trp Trp Pro Asp Trp Thr Ala Trp 
        835                 840                 845             


Leu Ala Gly Gln Ala Gly Ala Lys Arg Ala Ala Pro Ala Asn Tyr Gly 
    850                 855                 860                 


Asn Ala Arg Tyr Arg Ala Ile Glu Pro Ala Pro Gly Arg Tyr Val Lys 
865                 870                 875                 880 


Ala Lys Ala His Met Val Leu Ala Val Ala Ile Asp Lys Arg Gly Gly 
                885                 890                 895     


Gly Gly Gly Leu Glu Ser Gln His Asp Glu Ala Val Asp Asn Lys Phe 
            900                 905                 910         


Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn 
        915                 920                 925             


Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp 
    930                 935                 940                 


Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp 
945                 950                 955                 960 


Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn 
                965                 970                 975     


Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg 
            980                 985                 990         


Asn Ala Phe Ile Gln Ser Leu Lys  Asp Asp Pro Ser Gln  Ser Ala Asn 
        995                 1000                 1005             


Leu Leu  Ala Glu Ala Lys Lys  Leu Asn Asp Ala Gln  Ala Pro Lys 
    1010                 1015                 1020             


Val Asp  Ala Asn Ser Ser Ser  Val Pro Gly Asp Pro  Ala Ala Asn 
    1025                 1030                 1035             


Lys Ala  Arg Lys Glu Ala Glu  Leu Ala Ala Ala Thr  Ala Glu Gln 
    1040                 1045                 1050             


<210>  4
<211>  3162
<212>  DNA
<213>  Synthetic - encodes SN-P3 fusion polypeptide constructed in laboratory

<400>  4
atgagcccgc atccgcatcc gcgccatcat cataccctcg agcgtacggg aggcggtgga       60

ggcttcgaag cgcaacacga tgaagccgta gacaacaaat tcaacaaaga acaacaaaac      120

gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc      180

caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta      240

aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca aaacgcgttc      300

tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt      360

ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat      420

gctcaggcgc cgaaagtaga cgcgaattcg agctcggtac cccgtacggg aggcggtgga      480

ggcttcgaag cgcaacacga tgaagccgta gacaacaaat tcaacaaaga acaacaaaac      540

gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc      600

caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta      660

aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca aaacgcgttc      720

tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt      780

ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat      840

gctcaggcgc cgaaagtaga cgcgaattcg agccctaggc atatggcgac cggcaaaggc      900

gcggcagctt ccacgcagga aggcaagtcc caaccattca aggtcacgcc ggggccattc      960

gatccagcca catggctgga atggtcccgc cagtggcagg gcactgaagg caacggccac     1020

gcggccgcgt ccggcattcc gggcctggat gcgctggcag gcgtcaagat cgcgccggcg     1080

cagctgggtg atatccagca gcgctacatg aaggacttct cagcgctgtg gcaggccatg     1140

gccgagggca aggccgaggc caccggtccg ctgcacgacc ggcgcttcgc cggcgacgca     1200

tggcgcacca acctcccata tcgcttcgct gccgcgttct acctgctcaa tgcgcgcgcc     1260

ttgaccgagc tggccgatgc cgtcgaggcc gatgccaaga cccgccagcg catccgcttc     1320

gcgatctcgc aatgggtcga tgcgatgtcg cccgccaact tccttgccac caatcccgag     1380

gcgcagcgcc tgctgatcga gtcgggcggc gaatcgctgc gtgccggcgt gcgcaacatg     1440

atggaagacc tgacacgcgg caagatctcg cagaccgacg agagcgcgtt tgaggtcggc     1500

cgcaatgtcg cggtgaccga aggcgccgtg gtcttcgaga acgagtactt ccagctgttg     1560

cagtacaagc cgctgaccga caaggtgcac gcgcgcccgc tgctgatggt gccgccgtgc     1620

atcaacaagt actacatcct ggacctgcag ccggagagct cgctggtgcg ccatgtggtg     1680

gagcagggac atacggtgtt tctggtgtcg tggcgcaatc cggacgccag catggccggc     1740

agcacctggg acgactacat cgagcacgcg gccatccgcg ccatcgaagt cgcgcgcgac     1800

atcagcggcc aggacaagat caacgtgctc ggcttctgcg tgggcggcac cattgtctcg     1860

accgcgctgg cggtgctggc cgcgcgcggc gagcacccgg ccgccagcgt cacgctgctg     1920

accacgctgc tggactttgc cgacacgggc atcctcgacg tctttgtcga cgagggccat     1980

gtgcagttgc gcgaggccac gctgggcggc ggcgccggcg cgccgtgcgc gctgctgcgc     2040

ggccttgagc tggccaatac cttctcgttc ttgcgcccga acgacctggt gtggaactac     2100

gtggtcgaca actacctgaa gggcaacacg ccggtgccgt tcgacctgct gttctggaac     2160

ggcgacgcca ccaacctgcc ggggccgtgg tactgctggt acctgcgcca cacctacctg     2220

cagaacgagc tcaaggtacc gggcaagctg accgtgtgcg gcgtgccggt ggacctggcc     2280

agcatcgacg tgccgaccta tatctacggc tcgcgcgaag accatatcgt gccgtggacc     2340

gcggcctatg cctcgaccgc gctgctggcg aacaagctgc gcttcgtgct gggtgcgtcg     2400

ggccatatcg ccggtgtgat caacccgccg gccaagaaca agcgcagcca ctggactaac     2460

gatgcgctgc cggagtcgcc gcagcaatgg ctggccggcg ccatcgagca tcacggcagc     2520

tggtggccgg actggaccgc atggctggcc gggcaggccg gcgcgaaacg cgccgcgccc     2580

gccaactatg gcaatgcgcg ctatcgcgca atcgaacccg cgcctgggcg atacgtcaaa     2640

gccaaggcac atatggtgct ggcggtggcg attgataaac gcggaggcgg tggaggcctc     2700

gagtcgcaac acgatgaagc cgtagacaac aaattcaaca aagaacaaca aaacgcgttc     2760

tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt     2820

ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat     2880

gctcaggcgc cgaaagtaga caacaaattc aacaaagaac aacaaaacgc gttctatgag     2940

atcttacatt tacctaactt aaacgaagaa caacgaaacg ccttcatcca aagtttaaaa     3000

gatgacccaa gccaaagcgc taacctttta gcagaagcta aaaagctaaa tgatgctcag     3060

gcgccgaaag tagacgcgaa ttcgagctcg gtacccgggg atccggctgc taacaaagcc     3120

cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aa                        3162


<210>  5
<211>  7075
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid pET-14b PhaC-(GB1)3 comprising 3 x GB1 domain

<400>  5
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata      240

tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg      300

ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac      360

cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga      420

cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact      480

cagcttcctt tcgggctttg ttagcagccg gatcctcatt ccggtttttc cgtgacggta      540

aacgttttgg ttgcatcgtc ataggtccat tcaccatcaa cgccgttgtc attggcgtac      600

tgtttgaaca ctttttcggc cgtggcggca tccacggctt cggtcgtggt ttcacctttc      660

agcgttttgc cgttcagaat cagtttgtac gtgtcggtct tgctgccacc gccaccactg      720

ccaccgccag aaccgccacc gctctcaggc ttctccgtca cggtaaacgt tttggtcgca      780

tcatcgtacg tccattcacc gtcaacgccg ttgtcatttg cgtactgttt aaacactttt      840

tccgcggtcg ctgcatccac tgcttccgtg gtcgtttcgc ctttcagggt tttaccattc      900

agaatcagtt tgtaggtatc cgttttagag ccaccgccac cactgccacc gccagaaccg      960

ccaccgcttt ccggtttttc ggtaaccgtg aaggttttcg tggcatcatc gtaggtccat     1020

tcgccatcca caccattatc gttcgcgtac tgtttgaaaa ctttttctgc ggttgctgca     1080

tccactgctt cggtcgtggt ttcgcctttc agcgttttac cgttcaggat cagtttatag     1140

gtatccgttt tgctgccacc gccaccactg ccaccgccag aaccgccacc gctgccaccg     1200

ccaccgccac gtttatcaat aaccgccagc acctcgagtg ccttggcttt gacgtatcgc     1260

ccaggcgcgg gttcgattgc gcgatagcgc gcattgccat agttggcggg cgcggcgcgt     1320

ttcgcgccgg cctgcccggc cagccatgcg gtccagtccg gccaccagct gccgtgatgc     1380

tcgatggcgc cggccagcca ttgctgcggc gactccggca gcgcatcgtt agtccagtgg     1440

ctgcgcttgt tcttggccgg cgggttgatc acaccggcga tatggcccga cgcacccagc     1500

acgaagcgca gcttgttcgc cagcagcgcg gtcgaggcat aggccgcggt ccacggcacg     1560

atatggtctt cgcgcgagcc gtagatatag gtcggcacgt cgatgctggc caggtccacc     1620

ggcacgccgc acacggtcag cttgcccggt accttgagct cgttctgcag gtaggtgtgg     1680

cgcaggtacc agcagtacca cggccccggc aggttggtgg cgtcgccgtt ccagaacagc     1740

aggtcgaacg gcaccggcgt gttgcccttc aggtagttgt cgaccacgta gttccacacc     1800

aggtcgttcg ggcgcaagaa cgagaaggta ttggccagct caaggccgcg cagcagcgcg     1860

cacggcgcgc cggcgccgcc gcccagcgtg gcctcgcgca actgcacatg gccctcgtcg     1920

acaaagacgt cgaggatgcc cgtgtcggca aagtccagca gcgtggtcag cagcgtgacg     1980

ctggcggccg ggtgctcgcc gcgcgcggcc agcaccgcca gcgcggtcga gacaatggtg     2040

ccgcccacgc agaagccgag cacgttgatc ttgtcctggc cgctgatgtc gcgcgcgact     2100

tcgatggcgc ggatggccgc gtgctcgatg tagtcgtccc aggtgctgcc ggccatgctg     2160

gcgtccggat tgcgccacga caccagaaac accgtatgtc cctgctccac cacatggcgc     2220

accagcgagc tctccggctg caggtccagg atgtagtact tgttgatgca cggcggcacc     2280

atcagcagcg ggcgcgcgtg caccttgtcg gtcagcggct tgtactgcaa cagctggaag     2340

tactcgttct cgaagaccac ggcgccttcg gtcaccgcga cattgcggcc gacctcaaac     2400

gcgctctcgt cggtctgcga gatcttgccg cgtgtcaggt cttccatcat gttgcgcacg     2460

ccggcacgca gcgattcgcc gcccgactcg atcagcaggc gctgcgcctc gggattggtg     2520

gcaaggaagt tggcgggcga catcgcatcg acccattgcg agatcgcgaa gcggatgcgc     2580

tggcgggtct tggcatcggc ctcgacggca tcggccagct cggtcaaggc gcgcgcattg     2640

agcaggtaga acgcggcagc gaagcgatat gggaggttgg tgcgccatgc gtcgccggcg     2700

aagcgccggt cgtgcagcgg accggtggcc tcggccttgc cctcggccat ggcctgccac     2760

agcgctgaga agtccttcat gtagcgctgc tggatatcac ccagctgcgc cggcgcgatc     2820

ttgacgcctg ccagcgcatc caggcccgga atgccggacg cggccgcgtg gccgttgcct     2880

tcagtgccct gccactggcg ggaccattcc agccatgtgg ctggatcgaa tggccccggc     2940

gtgaccttga atggttggga cttgccttcc tgcgtggaag ctgccgcgcc tttgccggtc     3000

gccatactag tatctcctta tttctagagg gaaaccgttg tggtctccct atagtgagtc     3060

gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat     3120

caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga     3180

tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc     3240

cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt     3300

gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga     3360

gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg     3420

catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt     3480

gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat     3540

cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg     3600

tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc     3660

gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat     3720

tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt     3780

agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc     3840

gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg     3900

gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg     3960

tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc     4020

accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac     4080

ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg     4140

ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg     4200

ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg     4260

tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt     4320

ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg     4380

atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag     4440

cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt     4500

ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc     4560

atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag     4620

gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag     4680

acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt     4740

gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat     4800

gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg     4860

gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc     4920

gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat     4980

cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt     5040

aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc     5100

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     5160

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     5220

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     5280

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     5340

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     5400

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     5460

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     5520

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     5580

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     5640

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     5700

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     5760

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     5820

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     5880

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     5940

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     6000

tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     6060

atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc     6120

tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc     6180

aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc     6240

catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt     6300

gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc     6360

ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa     6420

aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt     6480

atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg     6540

cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc     6600

gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa     6660

agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt     6720

gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt     6780

caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag     6840

ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta     6900

tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     6960

aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat     7020

catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaa          7075


<210>  6
<211>  28
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic N-terminal linker for (GB1)3 domain

<400>  6

Leu Glu Val Leu Ala Val Ile Asp Lys Arg Gly Gly Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly 
            20                  25              


<210>  7
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic - linker for (GB1)3 domain

<400>  7

Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  


