                         SEQUENCE LISTING

<110>  Sinomab Bioscience Limited
 
<120>  ANTI-IDIOTYPIC ANTIBODIES AND USES THEREOF

<130>  SBL-005PCT

<140>  61/660,327
<141>  2012-06-15

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-1 heavy chain variable sequence from scFv phage #1

<400>  1

Asn Tyr Val Thr His 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-2 heavy chain variable sequence for scFv phage #2

<400>  2

Tyr Ile Asn Pro Tyr Ser Asp Gly Ser Lys Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-3 heavy chain variable sequence for scFv phage #3

<400>  3

Gly Lys Thr Glu Trp Phe Pro Tyr 
1               5               


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-1 light chain variable sequence for scFv phage #1

<400>  4

Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr Met Asn 
1               5                   10                  15  


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-2 light chain variable sequence for scFv phage #2

<400>  5

Ala Ala Ser Asn Leu Glu Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-3 light chain variable sequence for scFv phage #3


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X may be F or Y

<400>  6

Gln Gln Ser Asn Lys Asp Pro Xaa Thr 
1               5                   


<210>  7
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv for phage #3

<400>  7

Gln Val Gln Leu Val Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Val Thr His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Ser Asp Gly Ser Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Val Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Thr Glu Trp Phe Pro Tyr Trp Gly Gln Gly Thr Pro 
            100                 105                 110         


Leu Thr Val Ser Gly Gly Gly Gly Ser Gly Ser Gly Ser Ser Gly Gly 
        115                 120                 125             


Gly Gly Asp Ile Val Leu Thr Gln Thr Pro Ala Ser Leu Ala Val Ser 
    130                 135                 140                 


Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp 
145                 150                 155                 160 


Tyr Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln 
                165                 170                 175     


Pro Pro Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly Ile 
            180                 185                 190         


Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn 
        195                 200                 205             


Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 


Ser Asn Glu Asp Pro Phe Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 


<210>  8
<211>  1344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  heavy chain of the anti-idiotype mIgG expressed in clone AE6

<400>  8
caggtccaac tgcagcagtc tggacctgag ctggtaaagc ctggggcttc agtgaagatg       60

tcctgcaagg cttctggata cacattcact aactatgtta cgcactgggt gaagcagaag      120

cctgggcagg gccttgagtg gattggatat attaatcctt acagtgatgg ttctaagtac      180

aatgagaagt tcaaaggcaa ggccacactg acttcagacg aatcctccag cacagcctac      240

atggagctca gcagcctgac ctctgaggac tctgcggtct attactgtgc aagagggaaa      300

accgagtggt ttccttactg gggtcaaggc actacggtca ccgtctcctc agccaaaaca      360

acagccccat cggtctatcc actggcccct gtgtgtggag atacaactgg ctcttcggtg      420

actctaggat gcctggtcaa gggttatttc cctgagccag tgaccttgac ctggaactct      480

ggatccctgt ccagtggtgt gcacaccttc ccagctgtcc tgcagtctga cctctacacc      540

ctcagcagct cagtgactgt aacctcgagc acctggccca gccagtccat cacctgcaat      600

gtggcccacc cggcaagcag caccaaggtg gacaagaaaa ttgagcccag agggcccaca      660

atcaagccct gtcctccatg caaatgccca gcacctaacc tcttgggtgg accatccgtc      720

ttcatcttcc ctccaaagat caaggatgta ctcatgatct ccctgagccc catagtcaca      780

tgtgtggtgg tggatgtgag cgaggatgac ccagatgtcc agatcagctg gtttgtgaac      840

aacgtggaag tacacacagc tcagacacaa acccatagag aggattacaa cagtactctc      900

cgggtggtca gtgccctccc catccagcac caggactgga tgagtggcaa ggagttcaaa      960

tgcaaggtca acaacaaaga cctcccagcg cccatcgaga gaaccatctc aaaacccaaa     1020

gggtcagtaa gagctccaca ggtatatgtc ttgcctccac cagaagaaga gatgactaag     1080

aaacaggtca ctctgacctg catggtcaca gacttcatgc ctgaagacat ttacgtggag     1140

tggaccaaca acgggaaaac agagctaaac tacaagaaca ctgaaccagt cctggactct     1200

gatggttctt acttcatgta cagcaagctg agagtggaaa agaagaactg ggtggaaaga     1260

aatagctact cctgttcagt ggtccacgag ggtctgcaca atcaccttac gactaagacc     1320

atctcccggt ctctgggtaa ataa                                            1344


<210>  9
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  translated heavy chain of the anti-idiotype mIgG expressed in 
       clone AE6

<400>  9

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Val Thr His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Ser Asp Gly Ser Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Thr Glu Trp Phe Pro Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu 
        115                 120                 125             


Ala Pro Val Cys Gly Asp Thr Thr Gly Ser Ser Val Thr Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Leu Thr Trp Asn Ser 
145                 150                 155                 160 


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Thr Ser Ser Thr Trp 
            180                 185                 190         


Pro Ser Gln Ser Ile Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Ile Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys 
    210                 215                 220                 


Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser 
                245                 250                 255     


Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp 
            260                 265                 270         


Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln 
        275                 280                 285             


Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser 
    290                 295                 300                 


Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys 
305                 310                 315                 320 


Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile 
                325                 330                 335     


Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro 
            340                 345                 350         


Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met 
        355                 360                 365             


Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn 
    370                 375                 380                 


Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn 
                405                 410                 415     


Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu 
            420                 425                 430         


His Asn His Leu Thr Thr Lys Thr Ile Ser Arg Ser Leu Gly Lys 
        435                 440                 445         


<210>  10
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  light chain of the anti-idiotype mIgG expressed in clone AE6

<400>  10
gatattgttc tcacccagac tccagcttct ttggctgtgt ctctagggca gagggccacc       60

atctcctgca aggccagcca aagtgttgat tatgatggtg atagttatat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctatg ctgcatccaa tctagaatct      180

gggatcccag ccaggtttag tggcagtggg tctgggacag acttcaccct caacatccat      240

cctgtggagg aggaggatgc tgcaacctat tactgtcagc aaagtaatga ggatccattc      300

acgttcggag gtgggacaaa attggaaata aaacgacggg ctgatgctgc accaactgta      360

tccatcttcc caccatccag tgagcagtta acatctggag gtgcctcagt cgtgtgcttc      420

ttgaacaact tctaccccaa agacatcaat gtcaagtgga agattgatgg cagtgaacga      480

caaaatggcg tcctgaacag ttggactgat caggacagca aagacagcac ctacagcatg      540

agcagcaccc tcacgttgac caaggacgag tatgaacgac ataacagcta tacctgtgag      600

gccactcaca agacatcaac ttcacccatt gtcaagagct tcaacaggaa tgagtgttaa      660


<210>  11
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  translated light chain of the anti-idiotype mIgG expressed in 
       clone AE6

<400>  11

Asp Ile Val Leu Thr Gln Thr Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215                 


<210>  12
<211>  502
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine IgD transmembrane region fused with the CH3 domain of 
       anti-idiotype mIgG

<400>  12

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Val Thr His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Ser Asp Gly Ser Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Thr Glu Trp Phe Pro Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu 
        115                 120                 125             


Ala Pro Val Cys Gly Asp Thr Thr Gly Ser Ser Val Thr Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Leu Thr Trp Asn Ser 
145                 150                 155                 160 


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Thr Ser Ser Thr Trp 
            180                 185                 190         


Pro Ser Gln Ser Ile Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Ile Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys 
    210                 215                 220                 


Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser 
                245                 250                 255     


Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp 
            260                 265                 270         


Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln 
        275                 280                 285             


Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser 
    290                 295                 300                 


Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys 
305                 310                 315                 320 


Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile 
                325                 330                 335     


Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro 
            340                 345                 350         


Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met 
        355                 360                 365             


Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn 
    370                 375                 380                 


Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn 
                405                 410                 415     


Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu 
            420                 425                 430         


His Asn His Leu Thr Thr Lys Thr Ile Ser Arg Ser Leu Gly Lys Gly 
        435                 440                 445             


Ile Val Asn Thr Ile Gln His Ser Cys Ile Met Asp Glu Gln Ser Asp 
    450                 455                 460                 


Ser Tyr Met Asp Leu Glu Glu Glu Asn Gly Leu Trp Pro Thr Met Cys 
465                 470                 475                 480 


Thr Phe Val Ala Leu Phe Leu Leu Thr Leu Leu Tyr Ser Gly Phe Val 
                485                 490                 495     


Thr Phe Ile Lys Val Lys 
            500         


<210>  13
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-idiotype mIgG with membrane bound murine IgD

<400>  13

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Val Thr His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Ser Asp Gly Ser Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Thr Glu Trp Phe Pro Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Gly Asp Lys Lys Glu Pro Asp Met Phe Leu Leu 
        115                 120                 125             


Ser Glu Cys Lys Ala Pro Glu Glu Asn Glu Lys Ile Asn Leu Gly Cys 
    130                 135                 140                 


Leu Val Ile Gly Ser Gln Pro Leu Lys Ile Ser Trp Glu Pro Lys Lys 
145                 150                 155                 160 


Ser Ser Ile Val Glu His Val Phe Pro Ser Glu Met Arg Asn Gly Asn 
                165                 170                 175     


Tyr Thr Met Val Leu Gln Val Thr Val Leu Ala Ser Glu Leu Asn Leu 
            180                 185                 190         


Asn His Thr Cys Thr Ile Asn Lys Pro Lys Arg Lys Glu Lys Pro Phe 
        195                 200                 205             


Lys Phe Pro Glu Ser Trp Asp Ser Gln Ser Ser Lys Arg Val Thr Pro 
    210                 215                 220                 


Thr Leu Gln Ala Lys Asn His Ser Thr Glu Ala Thr Lys Ala Ile Thr 
225                 230                 235                 240 


Thr Lys Lys Asp Ile Glu Gly Ala Met Ala Pro Ser Asn Leu Thr Val 
                245                 250                 255     


Asn Ile Leu Thr Thr Ser Thr His Pro Glu Met Ser Ser Trp Leu Leu 
            260                 265                 270         


Cys Glu Val Ser Gly Phe Phe Pro Glu Asn Ile His Leu Met Trp Leu 
        275                 280                 285             


Gly Val His Ser Lys Met Lys Ser Thr Asn Phe Val Thr Ala Asn Pro 
    290                 295                 300                 


Thr Ala Gln Pro Gly Gly Thr Phe Gln Thr Trp Ser Val Leu Arg Leu 
305                 310                 315                 320 


Pro Val Ala Leu Ser Ser Ser Leu Asp Thr Tyr Thr Cys Val Val Glu 
                325                 330                 335     


His Glu Ala Ser Lys Thr Lys Leu Asn Ala Ser Lys Ser Leu Ala Ile 
            340                 345                 350         


Ser Gly Ile Val Asn Thr Ile Gln His Ser Cys Ile Met Asp Glu Gln 
        355                 360                 365             


Ser Asp Ser Tyr Met Asp Leu Glu Glu Glu Asn Gly Leu Trp Pro Thr 
    370                 375                 380                 


Met Cys Thr Phe Val Ala Leu Phe Leu Leu Thr Leu Leu Tyr Ser Gly 
385                 390                 395                 400 


Phe Val Thr Phe Ile Lys Val Lys 
                405             


<210>  14
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain Fd region of the anti-idiotype mIgG fused to the 
       transmembrane and cytoplasmic regions of Glycophorin A protein

<400>  14

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Val Thr His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Ser Asp Gly Ser Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Thr Glu Trp Phe Pro Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu 
        115                 120                 125             


Ala Pro Val Cys Gly Asp Thr Thr Gly Ser Ser Val Thr Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Leu Thr Trp Asn Ser 
145                 150                 155                 160 


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Thr Ser Ser Thr Trp 
            180                 185                 190         


Pro Ser Gln Ser Ile Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Ile Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys 
    210                 215                 220                 


Pro Pro Cys Lys Cys Pro Ala Pro Gly Glu Arg Val Gln Leu Ala His 
225                 230                 235                 240 


His Phe Ser Glu Pro Glu Ile Thr Leu Ile Ile Phe Gly Val Met Ala 
                245                 250                 255     


Gly Val Ile Gly Thr Ile Leu Leu Ile Ser Tyr Gly Ile Arg Arg Leu 
            260                 265                 270         


Ile Lys Lys Ser Pro Ser Asp Val Lys Pro Leu Pro Ser Pro Asp Thr 
        275                 280                 285             


Asp Val Pro Leu Ser Ser Val Glu Ile Glu Asn Pro Glu Thr Ser Asp 
    290                 295                 300                 


Gln 
305 


<210>  15
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain Fd region of the anti-idiotype mIgG fused to the 
       GPI-DAF sequence

<400>  15

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Val Thr His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Ser Asp Gly Ser Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Thr Glu Trp Phe Pro Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu 
        115                 120                 125             


Ala Pro Val Cys Gly Asp Thr Thr Gly Ser Ser Val Thr Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Leu Thr Trp Asn Ser 
145                 150                 155                 160 


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Thr Ser Ser Thr Trp 
            180                 185                 190         


Pro Ser Gln Ser Ile Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Ile Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys 
    210                 215                 220                 


Pro Pro Cys Lys Cys Pro Ala Pro Leu Thr Thr Ser Gly Ile Val Thr 
225                 230                 235                 240 


Met Ser His Gln Ala Leu Gly Phe Thr Leu Thr Gly Leu Leu Gly Thr 
                245                 250                 255     


Leu Val Thr Met Gly Leu Leu Thr 
            260                 


<210>  16
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv linker sequence

<400>  16

Gly Gly Gly Gly Ser Gly Ser Gly Ser Ser Gly Gly Gly Gly 
1               5                   10                  


<210>  17
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  portion of the murine IgD transmembrane (TM) sequence used for 
       fusion to the anti-idiotype mIgG

<400>  17

Gly Ile Val Asn Thr Ile Gln His Ser Cys Ile Met Asp Glu Gln Ser 
1               5                   10                  15      


Asp Ser Tyr Met Asp Leu Glu Glu Glu Asn Gly Leu Trp Pro Thr Met 
            20                  25                  30          


Cys Thr Phe Val Ala Leu Phe Leu Leu Thr Leu Leu Tyr Ser Gly Phe 
        35                  40                  45              


Val Thr Phe Ile Lys Val Lys 
    50                  55  


<210>  18
<211>  305
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence encoding a portion of the C-terminal end 
       of the anti-idiotype mIgG CH3 domain fused to the TM domain of 
       murine IgD

<400>  18
tgtacagcaa gctgagagtg gaaaagaaga actgggtgga aagaaatagc tactcctgtt       60

cagtggtcca cgagggtctg cacaatcacc acacgactaa gaccatctcc cggtctctgg      120

gtaaaggcat agtcaacacc atccaacact cgtgtatcat ggatgagcaa agtgacagct      180

acatggactt agaggaggag aacggcctgt ggcccacaat gtgcaccttc gtggccctct      240

tcctgctcac actgctctac agtggcttcg tcaccttcat caaggtgaag taagtgcgac      300

ggccg                                                                  305


<210>  19
<211>  290
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine IgD with the transmembrane (TM) sequence

<400>  19

Asp Lys Lys Glu Pro Asp Met Phe Leu Leu Ser Glu Cys Lys Ala Pro 
1               5                   10                  15      


Glu Glu Asn Glu Lys Ile Asn Leu Gly Cys Leu Val Ile Gly Ser Gln 
            20                  25                  30          


Pro Leu Lys Ile Ser Trp Glu Pro Lys Lys Ser Ser Ile Val Glu His 
        35                  40                  45              


Val Phe Pro Ser Glu Met Arg Asn Gly Asn Tyr Thr Met Val Leu Gln 
    50                  55                  60                  


Val Thr Val Leu Ala Ser Glu Leu Asn Leu Asn His Thr Cys Thr Ile 
65                  70                  75                  80  


Asn Lys Pro Lys Arg Lys Glu Lys Pro Phe Lys Phe Pro Glu Ser Trp 
                85                  90                  95      


Asp Ser Gln Ser Ser Lys Arg Val Thr Pro Thr Leu Gln Ala Lys Asn 
            100                 105                 110         


His Ser Thr Glu Ala Thr Lys Ala Ile Thr Thr Lys Lys Asp Ile Glu 
        115                 120                 125             


Gly Ala Met Ala Pro Ser Asn Leu Thr Val Asn Ile Leu Thr Thr Ser 
    130                 135                 140                 


Thr His Pro Glu Met Ser Ser Trp Leu Leu Cys Glu Val Ser Gly Phe 
145                 150                 155                 160 


Phe Pro Glu Asn Ile His Leu Met Trp Leu Gly Val His Ser Lys Met 
                165                 170                 175     


Lys Ser Thr Asn Phe Val Thr Ala Asn Pro Thr Ala Gln Pro Gly Gly 
            180                 185                 190         


Thr Phe Gln Thr Trp Ser Val Leu Arg Leu Pro Val Ala Leu Ser Ser 
        195                 200                 205             


Ser Leu Asp Thr Tyr Thr Cys Val Val Glu His Glu Ala Ser Lys Thr 
    210                 215                 220                 


Lys Leu Asn Ala Ser Lys Ser Leu Ala Ile Ser Gly Ile Val Asn Thr 
225                 230                 235                 240 


Ile Gln His Ser Cys Ile Met Asp Glu Gln Ser Asp Ser Tyr Met Asp 
                245                 250                 255     


Leu Glu Glu Glu Asn Gly Leu Trp Pro Thr Met Cys Thr Phe Val Ala 
            260                 265                 270         


Leu Phe Leu Leu Thr Leu Leu Tyr Ser Gly Phe Val Thr Phe Ile Lys 
        275                 280                 285             


Val Lys 
    290 


<210>  20
<211>  334
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coding sequence for membrane bound form of murine IgD

<400>  20
gccggcacca cctctcttgc agccaacttc actatctgtc ttgcaggtga taaaaaggaa       60

cctgacatgt tcctcctctc agagtgcaaa gccccagagg aaaatgaaaa gataaacctg      120

ggctgtttag taattggaag tcagccactg aaaatcagct gggagccaaa gaagtcaagt      180

atagttgaac atgtcttccc ctctgaaatg agaaatggca attatacaat ggtcctccag      240

gtcactgtgc tggcctcaga actgaacctc aaccacactt gcaccataaa taaacccaaa      300

aggaaagaaa aacctttcaa gtttcctgag tcat                                  334


<210>  21
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  portion of the Glycophorin A including the transmembrane region 
       used for fusion

<400>  21

Gly Glu Arg Val Gln Leu Ala His His Phe Ser Glu Pro Glu Ile Thr 
1               5                   10                  15      


Leu Ile Ile Phe Gly Val Met Ala Gly Val Ile Gly Thr Ile Leu Leu 
            20                  25                  30          


Ile Ser Tyr Gly Ile Arg Arg Leu Ile Lys Lys Ser Pro Ser Asp Val 
        35                  40                  45              


Lys Pro Leu Pro Ser Pro Asp Thr Asp Val Pro Leu Ser Ser Val Glu 
    50                  55                  60                  


Ile Glu Asn Pro Glu Thr Ser Asp Gln 
65                  70              


<210>  22
<211>  222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  portion of Glycophorin A sequence that includes the transmembrane
       region

<400>  22
ggcgagaggg tgcagctggc ccaccacttc agcgagcccg agatcaccct gatcatcttc       60

ggcgtgatgg ccggcgtgat cggcaccatc ctgctgatca gctacggcat caggaggctg      120

atcaagaaga gccccagcga cgtgaagccc ctgcccagcc ccgacaccga cgtgcccctg      180

agcagcgtgg agatcgagaa ccccgagacc agcgaccagt aa                         222


<210>  23
<211>  369
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic coding sequence for an anti-idiotype mIgG Fd fragment -
       Glycophorin A fusion

<400>  23
ctcgagcacc tggcccagcc agtccatcac ctgcaatgtg gcccacccgg caagcagcac       60

caaggtggac aagaaaattg agcccagagg gcccacaatc aagccctgtc ctccatgcaa      120

atgcccagca cctggcgaga gggtgcagct ggcccaccac ttcagcgagc ccgagatcac      180

cctgatcatc ttcggcgtga tggccggcgt gatcggcacc atcctgctga tcagctacgg      240

catcaggagg ctgatcaaga agagccccag cgacgtgaag cccctgccca gccccgacac      300

cgacgtgccc ctgagcagcg tggagatcga gaaccccgag accagcgacc agtaagtgcg      360

acggccggc                                                              369


<210>  24
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GPI-signal attached to a DAF hydrophobic domain

<400>  24

Leu Thr Thr Ser Gly Ile Val Thr Met Ser His Gln Ala Leu Gly Phe 
1               5                   10                  15      


Thr Leu Thr Gly Leu Leu Gly Thr Leu Val Thr Met Gly Leu Leu Thr 
            20                  25                  30          


<210>  25
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic coding sequence for GPI signal - DAF fusion

<400>  25
ctgaccacca gcggcatcgt gaccatgagc caccaggccc tgggcttcac cctgaccggc       60

ctgctgggca ccctggtgac catgggcctg ctgacc                                 96


<210>  26
<211>  244
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic sequence for anti-idiotype mIgG Fab-GPI-DAF fusion 
       protein

<400>  26
ctcgagcacc tggcccagcc agagcatcac ctgcaacgtg gcccaccccg ccagcagcac       60

caaggtggac aagaagatcg agcccagggg ccccaccatc aagccctgcc ccccctgcaa      120

gtgccccgcc cccctgacca ccagcggcat cgtgaccatg agccaccagg ccctgggctt      180

caccctgacc ggcctgctgg gcaccctggt gaccatgggc ctgctgacct aagtgcgacg      240

gccg                                                                   244


