                         SEQUENCE LISTING

<110>  Donovan, David M.
       Abaev, Igor V.
 
<120>  Staphylococcal Phage2638A Endolysin Amidase Domain is Lytic for 
       Staphylococcus aureus

<130>  D.N. 0045.10

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  1485
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated Phage 2638a

<400>  1
atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc       60

acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat      120

tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac      180

gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt      240

ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt      300

ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa      360

ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct      420

aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat      480

gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagctc      540

aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt      600

caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg      660

ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat      720

agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa      780

tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg      840

gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag      900

tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact      960

tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact     1020

aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga     1080

aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa     1140

aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt     1200

tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga     1260

tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt     1320

aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag     1380

ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag     1440

ttgtggggcg aaattaaact cgagcaccac caccaccacc actga                     1485


<210>  2
<211>  1071
<212>  DNA
<213>  Phage 2638a

<400>  2
cgccctaaag acgcaaagaa agatgaaaaa tcacaagtat gtagtggttt ggctatggaa       60

aaatatgaca ttacaaattt aaatgctaaa caagataaat caaagaatgg gagcgtgaaa      120

gagttgaaac atatctattc aaaccatatt aaaggtaaca agattacagc accaaaacct      180

agtattcaag gtgtggtcat ccacaatgat tatggtagta tgacacctag tcaatactta      240

ccatggttat atgcacgtga gaataacggt acacacgtta acggttgggc tagtgtttat      300

gcaaatagaa acgaagtgct ttggtatcat ccgacagact acgtagagtg gcattgtggt      360

aatcaatggg caaatgctaa cttaatcgga tttgaagtgt gtgagtcgta tcctggtaga      420

atctcggaca aattattctt agaaaatgaa gaagcgacat tgaaagtagc tgcggatgtg      480

atgaagtcgt acggattacc agttaatcgc aacactgtac gtctgcataa cgaattcttc      540

ggaacttctt gtccacatcg ttcgtgggac ttgcatgttg gcaaaggtga gccttacaca      600

actactaata ttaataaaat gaaagactac ttcatcaaac gcatcaaaca ttattatgac      660

ggtggaaagc tagaagtaag caaagcagca actatcaaac aatctgacgt taagcaagaa      720

gttaaaaagc aagaagcaaa acaaattgtg aaagcaacag attggaaaca gaataaagat      780

ggcatttggt ataaagctga acatgcttcg ttcacagtga cagcaccaga gggaattatc      840

acaagataca aaggtccttg gactggtcac ccacaagctg gtgtattaca aaaaggtcaa      900

acgattaaat atgatgaggt tcaaaaattt gacggtcatg tttgggtatc gtgggaaacg      960

tttgagggcg aaactgtata catgccggta cgcacatggg acgctaaaac tggtaaagtt     1020

ggtaagttgt ggggcgaaat taaactcgag caccaccacc accaccactg a              1071


<210>  3
<211>  948
<212>  DNA
<213>  Phage 2638a

<400>  3
atgaaacata tctattcaaa ccatattaaa ggtaacaaga ttacagcacc aaaacctagt       60

attcaaggtg tggtcatcca caatgattat ggtagtatga cacctagtca atacttacca      120

tggttatatg cacgtgagaa taacggtaca cacgttaacg gttgggctag tgtttatgca      180

aatagaaacg aagtgctttg gtatcatccg acagactacg tagagtggca ttgtggtaat      240

caatgggcaa atgctaactt aatcggattt gaagtgtgtg agtcgtatcc tggtagaatc      300

tcggacaaat tattcttaga aaatgaagaa gcgacattga aagtagctgc ggatgtgatg      360

aagtcgtacg gattaccagt taatcgcaac actgtacgtc tgcataacga attcttcgga      420

acttcttgtc cacatcgttc gtgggacttg catgttggca aaggtgagcc ttacacaact      480

actaatatta ataaaatgaa agactacttc atcaaacgca tcaaacatta ttatgacggt      540

ggaaagctag aagtaagcaa agcagcaact atcaaacaat ctgacgttaa gcaagaagtt      600

aaaaagcaag aagcaaaaca aattgtgaaa gcaacagatt ggaaacagaa taaagatggc      660

atttggtata aagctgaaca tgcttcgttc acagtgacag caccagaggg aattatcaca      720

agatacaaag gtccttggac tggtcaccca caagctggtg tattacaaaa aggtcaaacg      780

attaaatatg atgaggttca aaaatttgac ggtcatgttt gggtatcgtg ggaaacgttt      840

gagggcgaaa ctgtatacat gccggtacgc acatgggacg ctaaaactgg taaagttggt      900

aagttgtggg gcgaaattaa actcgagcac caccaccacc accactga                   948


<210>  4
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated Phage 2638a

<400>  4

Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser 
1               5                   10                  15      


Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg 
            20                  25                  30          


Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr 
        35                  40                  45              


His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala 
    50                  55                  60                  


Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly 
65                  70                  75                  80  


Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly 
                85                  90                  95      


His Leu Gln Arg Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn 
            100                 105                 110         


Gln Gly Asp Ile Ile Gly Leu Gln Gly Asn Ser Asn Tyr Tyr Asp Asn 
        115                 120                 125             


Pro Met Ser Val His Leu His Leu Gln Leu Arg Pro Lys Asp Ala Lys 
    130                 135                 140                 


Lys Asp Glu Lys Ser Gln Val Cys Ser Gly Leu Ala Met Glu Lys Tyr 
145                 150                 155                 160 


Asp Ile Thr Asn Leu Asn Ala Lys Gln Asp Lys Ser Lys Asn Gly Ser 
                165                 170                 175     


Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys 
            180                 185                 190         


Ile Thr Ala Pro Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp 
        195                 200                 205             


Tyr Gly Ser Met Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg 
    210                 215                 220                 


Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn 
225                 230                 235                 240 


Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His 
                245                 250                 255     


Cys Gly Asn Gln Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys 
            260                 265                 270         


Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu 
        275                 280                 285             


Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu 
    290                 295                 300                 


Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr 
305                 310                 315                 320 


Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro 
                325                 330                 335     


Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg 
            340                 345                 350         


Ile Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala 
        355                 360                 365             


Thr Ile Lys Gln Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala 
    370                 375                 380                 


Lys Gln Ile Val Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile 
385                 390                 395                 400 


Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly 
                405                 410                 415     


Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gln Ala Gly 
            420                 425                 430         


Val Leu Gln Lys Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe 
        435                 440                 445             


Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val 
    450                 455                 460                 


Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys 
465                 470                 475                 480 


Leu Trp Gly Glu Ile Lys Leu Glu His His His His His His 
                485                 490                 


<210>  5
<211>  357
<212>  PRT
<213>  Phage 2638a

<400>  5

Met Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys Ser Gln Val Cys Ser 
1               5                   10                  15      


Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn Leu Asn Ala Lys Gln 
            20                  25                  30          


Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser 
        35                  40                  45              


Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gln 
    50                  55                  60                  


Gly Val Val Ile His Asn Asp Tyr Gly Ser Met Thr Pro Ser Gln Tyr 
65                  70                  75                  80  


Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly 
                85                  90                  95      


Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro 
            100                 105                 110         


Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gln Trp Ala Asn Ala Asn 
        115                 120                 125             


Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp 
    130                 135                 140                 


Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp 
145                 150                 155                 160 


Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu 
                165                 170                 175     


His Asn Glu Phe Phe Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu 
            180                 185                 190         


His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met 
        195                 200                 205             


Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr Tyr Asp Gly Gly Lys 
    210                 215                 220                 


Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gln Ser Asp Val Lys Gln 
225                 230                 235                 240 


Glu Val Lys Lys Gln Glu Ala Lys Gln Ile Val Lys Ala Thr Asp Trp 
                245                 250                 255     


Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe 
            260                 265                 270         


Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp 
        275                 280                 285             


Thr Gly His Pro Gln Ala Gly Val Leu Gln Lys Gly Gln Thr Ile Lys 
    290                 295                 300                 


Tyr Asp Glu Val Gln Lys Phe Asp Gly His Val Trp Val Ser Trp Glu 
305                 310                 315                 320 


Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala 
                325                 330                 335     


Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Leu Glu His 
            340                 345                 350         


His His His His His 
        355         


<210>  6
<211>  315
<212>  PRT
<213>  Phage 2638a

<400>  6

Met Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala 
1               5                   10                  15      


Pro Lys Pro Ser Ile Gln Gly Val Val Ile His Asn Asp Tyr Gly Ser 
            20                  25                  30          


Met Thr Pro Ser Gln Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn 
        35                  40                  45              


Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu 
    50                  55                  60                  


Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn 
65                  70                  75                  80  


Gln Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr 
                85                  90                  95      


Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr 
            100                 105                 110         


Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn 
        115                 120                 125             


Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro 
    130                 135                 140                 


His Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr 
145                 150                 155                 160 


Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His 
                165                 170                 175     


Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys 
            180                 185                 190         


Gln Ser Asp Val Lys Gln Glu Val Lys Lys Gln Glu Ala Lys Gln Ile 
        195                 200                 205             


Val Lys Ala Thr Asp Trp Lys Gln Asn Lys Asp Gly Ile Trp Tyr Lys 
    210                 215                 220                 


Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr 
225                 230                 235                 240 


Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gln Ala Gly Val Leu Gln 
                245                 250                 255     


Lys Gly Gln Thr Ile Lys Tyr Asp Glu Val Gln Lys Phe Asp Gly His 
            260                 265                 270         


Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro 
        275                 280                 285             


Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly 
    290                 295                 300                 


Glu Ile Lys Leu Glu His His His His His His 
305                 310                 315 


<210>  7
<211>  30
<212>  DNA
<213>  Phage 2638a

<400>  7
taagaaggag atatacatat gctaactgct                                        30


<210>  8
<211>  24
<212>  DNA
<213>  Phage 2638a

<400>  8
ccttgaatac tctcgagtgg tgct                                              24


<210>  9
<211>  26
<212>  DNA
<213>  Phage 2638a

<400>  9
tctcacgtgc ctcgagccat ggtaag                                            26


<210>  10
<211>  25
<212>  DNA
<213>  Phage 2638a

<400>  10
ctgtcggatg atactcgagc acttc                                             25


<210>  11
<211>  25
<212>  DNA
<213>  Phage 2638a

<400>  11
ttacaattac gccatatgga cgcaa                                             25


<210>  12
<211>  24
<212>  DNA
<213>  Phage 2638a

<400>  12
atcaaacatc tcgaggacgg tgga                                              24


<210>  13
<211>  25
<212>  DNA
<213>  Phage 2638a

<400>  13
tccctctggc tcgagcactg tgaac                                             25


<210>  14
<211>  28
<212>  DNA
<213>  Phage 2638a

<400>  14
gtggtggtgg tgctcgagtt taatttcg                                          28


<210>  15
<211>  18
<212>  DNA
<213>  Phage 2638a

<400>  15
atcgacatat gctaactg                                                     18


<210>  16
<211>  23
<212>  DNA
<213>  Phage 2638a

<400>  16
gtggtgctcg agtttaattt cgc                                               23


<210>  17
<211>  26
<212>  DNA
<213>  Phage 2638a

<400>  17
gtgaaagagc tcaaacatat ctattc                                            26


<210>  18
<211>  26
<212>  DNA
<213>  Phage 2638a

<400>  18
gatatgtttg agctctttca cgctcc                                            26


<210>  19
<211>  27
<212>  DNA
<213>  Phage 2638a

<400>  19
gaggatcgag atctcgatcc cgcgaaa                                           27


<210>  20
<211>  25
<212>  DNA
<213>  Phage 2638a

<400>  20
cgtttagagg ccccaagggg ttatg                                             25


<210>  21
<211>  33
<212>  DNA
<213>  Phage 2638a

<400>  21
cgcgcgcgca tatgaaacat atctattcaa acc                                    33


<210>  22
<211>  6
<212>  PRT
<213>  Phage 2638a

<400>  22

Met Lys His Ile Tyr Ser 
1               5       


<210>  23
<211>  5
<212>  PRT
<213>  Phage 2638a

<400>  23

Lys His Ile Tyr Ser 
1               5   


<210>  24
<211>  14
<212>  DNA
<213>  Phage 2638a

<400>  24
aaaggaagga atta                                                         14


<210>  25
<211>  25
<212>  DNA
<213>  Phage 2638a

<400>  25
aaagaatggg agcgtgaaag agttg                                             25


<210>  26
<211>  13
<212>  RNA
<213>  Phage 2638a

<400>  26
auuccuccac uag                                                          13


<210>  27
<211>  48
<212>  DNA
<213>  Phage 2638a

<400>  27
aatgggagcg tgaaagagtt gaaacatatc tattcaaacc atattaaa                    48


<210>  28
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  28
cgcgcgcgca tatgaaacat atctattcaa ac                                     32


<210>  29
<211>  10
<212>  PRT
<213>  Phage 2638a

<400>  29

Met Lys His Ile Tyr Ser Asn His Ile Lys 
1               5                   10  


<210>  30
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  30
cctcgcactt tctcgagttt gtctag                                            26


<210>  31
<211>  46
<212>  DNA
<213>  Phage 2638a

<400>  31
gggagcgtga aaagagttga aacatatcta ttcaaaccat attaaa                      46


<210>  32
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  32
gtgaaagagc tcaaacatat ctattc                                            26


<210>  33
<211>  15
<212>  PRT
<213>  Phage 2638a

<400>  33

Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys 
1               5                   10                  15  


<210>  34
<211>  1485
<212>  DNA
<213>  Phage 2638a

<400>  34
atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc       60

acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat      120

tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac      180

gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt      240

ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt      300

ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa      360

ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct      420

aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat      480

gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg      540

aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt      600

caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg      660

ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat      720

agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa      780

tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg      840

gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag      900

tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact      960

tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact     1020

aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga     1080

aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa     1140

aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt     1200

tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga     1260

tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt     1320

aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag     1380

ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag     1440

ttgtggggcg aaattaaact cgagcaccac caccaccacc actga                     1485


