                         SEQUENCE LISTING

<110>  ACIB GmbH
 
<120>  Asymmetric Enzymatic Hydration of Hydroxystyrene Derivatives

<130>  AC001P

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-optimised sequence of HCHL

<400>  1

Met Ser Thr Tyr Glu Gly Arg Trp Lys Thr Val Lys Val Glu Ile Glu 
1               5                   10                  15      


Asp Gly Ile Ala Phe Val Ile Leu Asn Arg Pro Glu Lys Arg Asn Ala 
            20                  25                  30          


Met Ser Pro Thr Leu Asn Arg Glu Met Ile Asp Val Leu Glu Thr Leu 
        35                  40                  45              


Glu Gln Asp Pro Ala Ala Gly Val Leu Val Leu Thr Gly Ala Gly Glu 
    50                  55                  60                  


Ala Trp Thr Ala Gly Met Asp Leu Lys Glu Tyr Phe Arg Glu Val Asp 
65                  70                  75                  80  


Ala Gly Pro Glu Ile Leu Gln Glu Lys Ile Arg Arg Glu Ala Ser Gln 
                85                  90                  95      


Trp Gln Trp Lys Leu Leu Arg Met Tyr Ala Lys Pro Thr Ile Ala Met 
            100                 105                 110         


Val Asn Gly Trp Cys Phe Gly Gly Gly Phe Ser Pro Leu Val Ala Cys 
        115                 120                 125             


Asp Leu Ala Ile Cys Ala Asp Glu Ala Thr Phe Gly Leu Ser Glu Ile 
    130                 135                 140                 


Asn Trp Gly Ile Pro Pro Gly Asn Leu Val Ser Lys Ala Met Ala Asp 
145                 150                 155                 160 


Thr Val Gly His Arg Gln Ser Leu Tyr Tyr Ile Met Thr Gly Lys Thr 
                165                 170                 175     


Phe Gly Gly Gln Lys Ala Ala Glu Met Gly Leu Val Asn Glu Ser Val 
            180                 185                 190         


Pro Leu Ala Gln Leu Arg Glu Val Thr Ile Glu Leu Ala Arg Asn Leu 
        195                 200                 205             


Leu Glu Lys Asn Pro Val Val Leu Arg Ala Ala Lys His Gly Phe Lys 
    210                 215                 220                 


Arg Cys Arg Glu Leu Thr Trp Glu Gln Asn Glu Asp Tyr Leu Tyr Ala 
225                 230                 235                 240 


Lys Leu Asp Gln Ser Arg Leu Leu Asp Thr Glu Gly Gly Arg Glu Gln 
                245                 250                 255     


Gly Met Lys Gln Phe Leu Asp Asp Lys Ser Ile Lys Pro Gly Leu Gln 
            260                 265                 270         


Ala Tyr Lys Arg 
        275     


<210>  2
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-opimised sequence of PAD_Ba

<400>  2

Met Glu Asn Phe Ile Gly Ser His Met Ile Tyr Thr Tyr Glu Asn Gly 
1               5                   10                  15      


Trp Glu Tyr Glu Ile Tyr Ile Lys Asn Asp His Thr Ile Asp Tyr Arg 
            20                  25                  30          


Ile His Ser Gly Met Val Gly Gly Arg Trp Val Arg Asp Gln Glu Val 
        35                  40                  45              


Asn Ile Val Lys Leu Thr Glu Gly Val Tyr Lys Val Ser Trp Thr Glu 
    50                  55                  60                  


Pro Thr Gly Thr Asp Val Ser Leu Asn Phe Met Pro Asn Glu Lys Arg 
65                  70                  75                  80  


Met His Gly Ile Ile Phe Phe Pro Lys Trp Val His Glu His Pro Glu 
                85                  90                  95      


Ile Thr Val Cys Tyr Gln Asn Asp Tyr Ile Asp Val Met Lys Glu Ser 
            100                 105                 110         


Arg Glu Lys Tyr Asp Thr Tyr Pro Lys Tyr Val Val Pro Glu Phe Ala 
        115                 120                 125             


Asp Ile Thr Tyr Leu Asn Asn Ala Gly Ile Asn Asn Glu Ala Leu Ile 
    130                 135                 140                 


Ser Glu Ala Pro Tyr Glu Gly Met Thr Asp Asp Ile Arg Ala Gly Lys 
145                 150                 155                 160 


Leu Lys 
        


<210>  3
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-optimised sequence of PAD_Lp

<400>  3

Met Thr Lys Thr Phe Lys Thr Leu Asp Asp Phe Leu Gly Thr His Phe 
1               5                   10                  15      


Ile Tyr Thr Tyr Asp Asn Gly Trp Glu Tyr Glu Trp Tyr Ala Lys Asn 
            20                  25                  30          


Asp His Thr Val Asp Tyr Arg Ile His Gly Gly Met Val Ala Gly Arg 
        35                  40                  45              


Trp Val Thr Asp Gln Lys Ala Asp Ile Val Met Leu Thr Glu Gly Ile 
    50                  55                  60                  


Tyr Lys Ile Ser Trp Thr Glu Pro Thr Gly Thr Asp Val Ala Leu Asp 
65                  70                  75                  80  


Phe Met Pro Asn Glu Lys Lys Leu His Gly Thr Ile Phe Phe Pro Lys 
                85                  90                  95      


Trp Val Glu Glu His Pro Glu Ile Thr Val Thr Tyr Gln Asn Glu His 
            100                 105                 110         


Ile Asp Leu Met Glu Gln Ser Arg Glu Lys Tyr Ala Thr Tyr Pro Lys 
        115                 120                 125             


Leu Val Val Pro Glu Phe Ala Asn Ile Thr Tyr Met Gly Asp Ala Gly 
    130                 135                 140                 


Gln Asn Asn Glu Asp Val Ile Ser Glu Ala Pro Tyr Lys Glu Met Pro 
145                 150                 155                 160 


Asn Asp Ile Arg Asn Gly Lys Tyr Phe Asp Gln Asn Tyr His Arg Leu 
                165                 170                 175     


Asn Lys 
        


<210>  4
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-optimised sequence of FDC_Esp

<400>  4

Met Asn Thr Phe Asp Lys His Asp Leu Ser Gly Phe Val Gly Lys His 
1               5                   10                  15      


Leu Val Tyr Thr Tyr Asp Asn Gly Trp Asn Tyr Glu Ile Tyr Val Lys 
            20                  25                  30          


Asn Asp Asn Thr Ile Asp Tyr Arg Ile His Ser Gly Leu Val Gly Asn 
        35                  40                  45              


Arg Trp Val Lys Asp Gln Glu Ala Tyr Ile Val Arg Val Gly Glu Ser 
    50                  55                  60                  


Ile Tyr Lys Ile Ser Trp Thr Glu Pro Thr Gly Thr Asp Val Ser Leu 
65                  70                  75                  80  


Ile Val Asn Leu Gly Asp Ser Leu Phe His Gly Thr Ile Phe Phe Pro 
                85                  90                  95      


Arg Trp Val Met Asn Asn Pro Glu Pro Thr Val Cys Phe Gln Asn Asp 
            100                 105                 110         


His Ile Pro Leu Met Asn Ser Tyr Arg Glu Ala Gly Pro Ala Tyr Pro 
        115                 120                 125             


Thr Glu Val Ile Asp Glu Phe Ala Thr Ile Thr Phe Val Arg Asp Cys 
    130                 135                 140                 


Gly Ala Asn Asn Glu Ser Val Ile Ala Cys Ala Ala Ser Glu Leu Pro 
145                 150                 155                 160 


Lys Asn Phe Pro Asp Asn Leu Lys 
                165             


<210>  5
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-optimised sequence of PAD_Psp

<400>  5

Met Ser Thr Phe Asp Lys His Asp Leu Ser Gly Ile Val Gly Lys His 
1               5                   10                  15      


Leu Val Tyr Thr Tyr Asp Asn Gly Trp Asn Tyr Glu Leu Tyr Ile Lys 
            20                  25                  30          


Asn Ala Ser Thr Ile Asp Tyr Arg Ile His Ser Gly Met Val Gly Asn 
        35                  40                  45              


Arg Trp Val Lys Asn Gln His Val Tyr Val Val Arg Leu Ala Gln Asp 
    50                  55                  60                  


Val Tyr Lys Val Ser Trp Thr Glu Pro Thr Gly Thr Asp Val Ser Leu 
65                  70                  75                  80  


Ala Val Asn Leu Ala Asp Lys Ile Phe His Gly Thr Ile Phe Phe Pro 
                85                  90                  95      


Arg Trp Val Met Asn Asn Pro Glu Lys Thr Val Cys Phe Gln Asn Asp 
            100                 105                 110         


His Leu Glu Glu Met Ala Lys Phe Arg Glu Thr Gly Pro Ala Tyr Pro 
        115                 120                 125             


Thr Glu Ile Ile Asp Glu Phe Ala Thr Leu Thr Val Ile Arg Glu Val 
    130                 135                 140                 


Gly Glu Asn Asn Glu Ser Val Ile Asp Cys Pro Pro Asp Gln Leu Pro 
145                 150                 155                 160 


Ala Asn Trp Pro Ala Asn Leu Gln Asn 
                165                 


<210>  6
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-optimised sequence of PAD_Ll

<400>  6

Met Lys Thr Phe Lys Ser Leu Glu Asp Phe Val Gly Thr His Phe Ile 
1               5                   10                  15      


Tyr Thr Tyr Asp Asn Gly Trp Glu Tyr Glu Leu Tyr Val Lys Asn Asp 
            20                  25                  30          


His Thr Ile Asp Tyr Arg Ile His Gly Gly Met Val Ala Gly Arg Trp 
        35                  40                  45              


Val Lys Asp Gln Glu Val Ser Leu Val Met Leu Thr Glu Gly Ile Tyr 
    50                  55                  60                  


Lys Ile Thr Trp Thr Glu Pro Thr Gly Thr Asp Val Ala Leu Asp Phe 
65                  70                  75                  80  


Leu Pro Asn Glu Gly Lys Leu His Gly Met Ile Phe Phe Pro Lys Trp 
                85                  90                  95      


Val Glu Glu His Pro Glu Ile Thr Val Cys Phe Gln Asn Asp Phe Ile 
            100                 105                 110         


Asp Leu Met His Glu Ser Arg Glu Lys Tyr Glu Thr Tyr Pro Lys Tyr 
        115                 120                 125             


Leu Val Pro Glu Phe Ala Lys Ile Thr Tyr Ala Ala Glu Ala Gly Lys 
    130                 135                 140                 


Asp Asn Asp Asp Val Val Ala Gln Ala Pro Tyr Lys Glu Met Thr Asn 
145                 150                 155                 160 


Asp Ile Arg Asn Gly Lys Tyr Phe Asp Gln Asn Tyr Lys Met Ile Lys 
                165                 170                 175     


His 
    


<210>  7
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-optimised sequence of PAD_Mc

<400>  7

Met Thr Ser Val Thr Asn Pro Ile Pro Pro Gln Asp Leu Ser Gly Ile 
1               5                   10                  15      


Val Gly His Arg Phe Ile Tyr Thr Tyr Ala Asn Gly Trp Gln Tyr Glu 
            20                  25                  30          


Met Tyr Val Lys Asn Val Thr Thr Ile Asp Tyr Arg Ile His Ser Gly 
        35                  40                  45              


His Val Gly Gly Arg Trp Val Lys Gly Gln Gln Val Asn Leu Val Gln 
    50                  55                  60                  


Leu Asp Asp Asp Ser Phe Lys Ile Ser Trp Thr Glu Pro Thr Gly Thr 
65                  70                  75                  80  


Cys Val Ala Val Asn Val Leu Pro Gly Lys Arg Arg Ile His Gly Val 
                85                  90                  95      


Ile Phe Phe Pro Gln Trp Ile Arg Met His Gly Glu His Thr Val Cys 
            100                 105                 110         


Phe Gln Asn Asp His Leu Asp Glu Met Arg Ala Tyr Arg Asp Arg Gly 
        115                 120                 125             


Pro Thr Tyr Pro Ile Tyr Glu Val Pro Glu Phe Ala Tyr Ile Thr Leu 
    130                 135                 140                 


Phe Glu Tyr Val Gly Thr Asp Asp Glu Thr Val Ile Asp Thr Gly Pro 
145                 150                 155                 160 


Glu His Leu Pro Gln Gly Trp Ser Asn Arg Thr Asn 
                165                 170         


<210>  8
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Codon-optimised sequence of PAD_Msp

<400>  8

Met Ser Asp Leu Phe Ala Ser Thr Arg Arg Glu Glu Ile Ala Pro Phe 
1               5                   10                  15      


Leu Gly Lys His Phe Ile Tyr Thr Tyr Glu Asn Gly Trp Gln Tyr Glu 
            20                  25                  30          


Met Tyr Ile Lys Asn Glu Arg Thr Ile Asp Tyr Arg Ile His Ser Gly 
        35                  40                  45              


Ile Val Gly Gly Arg Trp Val Arg Asp Gln Val Ala His Ile Val Arg 
    50                  55                  60                  


Leu Ser Asp Glu Val Val Lys Ile Ser Trp Asp Glu Pro Thr Gly Thr 
65                  70                  75                  80  


Thr Val Ser Val Ala Val Asn Phe Glu Glu Arg Arg Ile His Gly Val 
                85                  90                  95      


Ile Phe Phe Pro Gln Trp Ile Ala Gln Asp Pro Lys Arg Thr Val Cys 
            100                 105                 110         


Phe Gln Asn Glu His Leu Asp Arg Met Arg Gln Tyr Arg Asp Ala Gly 
        115                 120                 125             


Pro Thr Tyr Pro Lys Leu Val Val Asp Glu Phe Ala Ser Val Thr Phe 
    130                 135                 140                 


Leu Glu Asp Cys Gly Ile Asp Asn Gln Glu Val Ile Ala Cys Ala Pro 
145                 150                 155                 160 


Ala Asp Leu Pro Asp Gly Tyr Ala Ala Arg Gln Asn 
                165                 170         


