                         SEQUENCE LISTING

<110>  Scil Proteins GmbH
 
<120>  HUMAN FUSION PROTEINS COMPRISING SINGLE CHAIN TNFALPHA AND 
       TARGETING DOMAINS

<130>  761-3 PCT

<150>  EP12171789.6
<151>  2012-06-13

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  2
<211>  91
<212>  PRT
<213>  Homo sapiens

<400>  2

Glu Val Pro Gln Leu Thr Asp Leu Ser Phe Val Asp Ile Thr Asp Ser 
1               5                   10                  15      


Ser Ile Gly Leu Arg Trp Thr Pro Leu Asn Ser Ser Thr Ile Ile Gly 
            20                  25                  30          


Tyr Arg Ile Thr Val Val Ala Ala Gly Glu Gly Ile Pro Ile Phe Glu 
        35                  40                  45              


Asp Phe Val Asp Ser Ser Val Gly Tyr Tyr Thr Val Thr Gly Leu Glu 
    50                  55                  60                  


Pro Gly Ile Asp Tyr Asp Ile Ser Val Ile Thr Leu Ile Asn Gly Gly 
65                  70                  75                  80  


Glu Ser Ala Pro Thr Thr Leu Thr Gln Gln Thr 
                85                  90      


<210>  3
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  3

Gly Ile Gly 
1           


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  4

Ser Gly Gly Gly Gly 
1               5   


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  5

Ser Gly Gly Gly Gly Ile Gly 
1               5           


<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  6

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ile Gly 
1               5                   10          


<210>  7
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  7

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  8

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  9
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  9

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10          


<210>  10
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ubiquitin start sequence (F45W, G75A, G76A)

<400>  10

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala 
65                  70                  75      


<210>  11
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ub2; dimer of unmodified ubiquitin start sequence with GIG linker

<400>  11

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 
    130                 135                 140                 


Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  12
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-ED-B affilin 54646

<400>  12

Met Thr Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  13
<211>  157
<212>  PRT
<213>  Homo sapiens

<400>  13

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  14
<211>  156
<212>  PRT
<213>  Mus musculus

<400>  14

Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn His Gln Val Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ala Asp Gly Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Asp Tyr Val Leu Leu Thr His Thr Val Ser 
65                  70                  75                  80  


Arg Phe Ala Ile Ser Tyr Gln Glu Lys Val Asn Leu Leu Ser Ala Val 
                85                  90                  95      


Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro 
            100                 105                 110         


Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly 
        115                 120                 125             


Asp Gln Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala 
    130                 135                 140                 


Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu 
145                 150                 155     


<210>  15
<211>  156
<212>  PRT
<213>  Rattus norvegicus

<400>  15

Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Val His Val 
1               5                   10                  15      


Val Ala Asn His Gln Ala Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ala Asp Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Asp Tyr Val Leu Leu Thr His Thr Val Ser 
65                  70                  75                  80  


Arg Phe Ala Thr Ser Tyr Gln Glu Lys Val Ser Leu Leu Ser Ala Ile 
                85                  90                  95      


Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro 
            100                 105                 110         


Trp Tyr Glu Pro Met Tyr Leu Gly Gly Val Ser Gln Leu Glu Lys Gly 
        115                 120                 125             


Asp Leu Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp Ile Thr 
    130                 135                 140                 


Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu 
145                 150                 155     


<210>  16
<211>  662
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  64179, fusion protein of Ub2 and scTNFalpha, murine

<400>  16

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 
    130                 135                 140                 


Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
                165                 170                 175     


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
            180                 185                 190         


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
        195                 200                 205             


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
    210                 215                 220                 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
225                 230                 235                 240 


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
                245                 250                 255     


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
            260                 265                 270         


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
        275                 280                 285             


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
    290                 295                 300                 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
305                 310                 315                 320 


Phe Gly Val Ile Ala Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly 
                325                 330                 335     


Gly Ser Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Ala 
            340                 345                 350         


His Val Val Ala Asn His Gln Val Glu Glu Gln Leu Glu Trp Leu Ser 
        355                 360                 365             


Gln Arg Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn 
    370                 375                 380                 


Gln Leu Val Val Pro Ala Asp Gly Leu Tyr Leu Val Tyr Ser Gln Val 
385                 390                 395                 400 


Leu Phe Lys Gly Gln Gly Cys Pro Asp Tyr Val Leu Leu Thr His Thr 
                405                 410                 415     


Val Ser Arg Phe Ala Ile Ser Tyr Gln Glu Lys Val Asn Leu Leu Ser 
            420                 425                 430         


Ala Val Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu 
        435                 440                 445             


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
    450                 455                 460                 


Lys Gly Asp Gln Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp 
465                 470                 475                 480 


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu Gly Gly 
                485                 490                 495     


Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
            500                 505                 510         


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
        515                 520                 525             


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
    530                 535                 540                 


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
545                 550                 555                 560 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
                565                 570                 575     


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
            580                 585                 590         


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
        595                 600                 605             


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
    610                 615                 620                 


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
625                 630                 635                 640 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
                645                 650                 655     


Phe Gly Val Ile Ala Leu 
            660         


<210>  17
<211>  662
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  64177, fusion protein of 54646 and scTNFalpha, murine

<400>  17

Met Thr Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
                165                 170                 175     


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
            180                 185                 190         


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
        195                 200                 205             


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
    210                 215                 220                 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
225                 230                 235                 240 


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
                245                 250                 255     


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
            260                 265                 270         


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
        275                 280                 285             


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
    290                 295                 300                 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
305                 310                 315                 320 


Phe Gly Val Ile Ala Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly 
                325                 330                 335     


Gly Ser Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Ala 
            340                 345                 350         


His Val Val Ala Asn His Gln Val Glu Glu Gln Leu Glu Trp Leu Ser 
        355                 360                 365             


Gln Arg Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn 
    370                 375                 380                 


Gln Leu Val Val Pro Ala Asp Gly Leu Tyr Leu Val Tyr Ser Gln Val 
385                 390                 395                 400 


Leu Phe Lys Gly Gln Gly Cys Pro Asp Tyr Val Leu Leu Thr His Thr 
                405                 410                 415     


Val Ser Arg Phe Ala Ile Ser Tyr Gln Glu Lys Val Asn Leu Leu Ser 
            420                 425                 430         


Ala Val Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu 
        435                 440                 445             


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
    450                 455                 460                 


Lys Gly Asp Gln Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp 
465                 470                 475                 480 


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu Gly Gly 
                485                 490                 495     


Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
            500                 505                 510         


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
        515                 520                 525             


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
    530                 535                 540                 


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
545                 550                 555                 560 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
                565                 570                 575     


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
            580                 585                 590         


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
        595                 600                 605             


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
    610                 615                 620                 


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
625                 630                 635                 640 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
                645                 650                 655     


Phe Gly Val Ile Ala Leu 
            660         


<210>  18
<211>  665
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  75808, fusion protein of 54646 and scTNFalpha, human

<400>  18

Met Thr Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Arg Ser Ser Ser Arg 
                165                 170                 175     


Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro Gln Ala 
            180                 185                 190         


Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala 
        195                 200                 205             


Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly 
    210                 215                 220                 


Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
225                 230                 235                 240 


Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala Val Ser 
                245                 250                 255     


Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln 
            260                 265                 270         


Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile 
        275                 280                 285             


Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala 
    290                 295                 300                 


Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val 
305                 310                 315                 320 


Tyr Phe Gly Ile Ile Ala Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly 
                325                 330                 335     


Gly Gly Ser Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val 
            340                 345                 350         


Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu 
        355                 360                 365             


Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp 
    370                 375                 380                 


Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln 
385                 390                 395                 400 


Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr 
                405                 410                 415     


His Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu 
            420                 425                 430         


Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala 
        435                 440                 445             


Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln 
    450                 455                 460                 


Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr 
465                 470                 475                 480 


Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
                485                 490                 495     


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Val Arg Ser Ser 
            500                 505                 510         


Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro 
        515                 520                 525             


Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu 
    530                 535                 540                 


Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser 
545                 550                 555                 560 


Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly 
                565                 570                 575     


Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala 
            580                 585                 590         


Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro 
        595                 600                 605             


Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu 
    610                 615                 620                 


Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu 
625                 630                 635                 640 


Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly 
                645                 650                 655     


Gln Val Tyr Phe Gly Ile Ile Ala Leu 
            660                 665 


<210>  19
<211>  662
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  83563, mutein (T2V) of fusion protein 64177

<400>  19

Met Val Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
                165                 170                 175     


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
            180                 185                 190         


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
        195                 200                 205             


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
    210                 215                 220                 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
225                 230                 235                 240 


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
                245                 250                 255     


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
            260                 265                 270         


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
        275                 280                 285             


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
    290                 295                 300                 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
305                 310                 315                 320 


Phe Gly Val Ile Ala Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly 
                325                 330                 335     


Gly Ser Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Ala 
            340                 345                 350         


His Val Val Ala Asn His Gln Val Glu Glu Gln Leu Glu Trp Leu Ser 
        355                 360                 365             


Gln Arg Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn 
    370                 375                 380                 


Gln Leu Val Val Pro Ala Asp Gly Leu Tyr Leu Val Tyr Ser Gln Val 
385                 390                 395                 400 


Leu Phe Lys Gly Gln Gly Cys Pro Asp Tyr Val Leu Leu Thr His Thr 
                405                 410                 415     


Val Ser Arg Phe Ala Ile Ser Tyr Gln Glu Lys Val Asn Leu Leu Ser 
            420                 425                 430         


Ala Val Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu 
        435                 440                 445             


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
    450                 455                 460                 


Lys Gly Asp Gln Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp 
465                 470                 475                 480 


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu Gly Gly 
                485                 490                 495     


Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
            500                 505                 510         


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
        515                 520                 525             


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
    530                 535                 540                 


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
545                 550                 555                 560 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
                565                 570                 575     


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
            580                 585                 590         


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
        595                 600                 605             


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
    610                 615                 620                 


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
625                 630                 635                 640 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
                645                 650                 655     


Phe Gly Val Ile Ala Leu 
            660         


<210>  20
<211>  665
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  h83563, mutein (T2V) of fusion protein 75808

<400>  20

Met Val Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Arg Ser Ser Ser Arg 
                165                 170                 175     


Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro Gln Ala 
            180                 185                 190         


Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala 
        195                 200                 205             


Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly 
    210                 215                 220                 


Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
225                 230                 235                 240 


Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala Val Ser 
                245                 250                 255     


Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln 
            260                 265                 270         


Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile 
        275                 280                 285             


Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala 
    290                 295                 300                 


Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val 
305                 310                 315                 320 


Tyr Phe Gly Ile Ile Ala Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly 
                325                 330                 335     


Gly Gly Ser Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val 
            340                 345                 350         


Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu 
        355                 360                 365             


Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp 
    370                 375                 380                 


Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln 
385                 390                 395                 400 


Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr 
                405                 410                 415     


His Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu 
            420                 425                 430         


Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala 
        435                 440                 445             


Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln 
    450                 455                 460                 


Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr 
465                 470                 475                 480 


Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
                485                 490                 495     


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Val Arg Ser Ser 
            500                 505                 510         


Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro 
        515                 520                 525             


Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu 
    530                 535                 540                 


Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser 
545                 550                 555                 560 


Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly 
                565                 570                 575     


Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala 
            580                 585                 590         


Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro 
        595                 600                 605             


Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu 
    610                 615                 620                 


Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu 
625                 630                 635                 640 


Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly 
                645                 650                 655     


Gln Val Tyr Phe Gly Ile Ile Ala Leu 
            660                 665 


<210>  21
<211>  662
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  83564, mutein (T2R/F63P) of fusion protein 64177

<400>  21

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
                165                 170                 175     


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
            180                 185                 190         


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
        195                 200                 205             


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
    210                 215                 220                 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
225                 230                 235                 240 


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
                245                 250                 255     


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
            260                 265                 270         


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
        275                 280                 285             


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
    290                 295                 300                 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
305                 310                 315                 320 


Phe Gly Val Ile Ala Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly 
                325                 330                 335     


Gly Ser Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Ala 
            340                 345                 350         


His Val Val Ala Asn His Gln Val Glu Glu Gln Leu Glu Trp Leu Ser 
        355                 360                 365             


Gln Arg Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn 
    370                 375                 380                 


Gln Leu Val Val Pro Ala Asp Gly Leu Tyr Leu Val Tyr Ser Gln Val 
385                 390                 395                 400 


Leu Phe Lys Gly Gln Gly Cys Pro Asp Tyr Val Leu Leu Thr His Thr 
                405                 410                 415     


Val Ser Arg Phe Ala Ile Ser Tyr Gln Glu Lys Val Asn Leu Leu Ser 
            420                 425                 430         


Ala Val Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu 
        435                 440                 445             


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
    450                 455                 460                 


Lys Gly Asp Gln Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp 
465                 470                 475                 480 


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu Gly Gly 
                485                 490                 495     


Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Leu Arg Ser Ser Ser Gln 
            500                 505                 510         


Asn Ser Ser Asp Lys Pro Val Ala His Val Val Ala Asn His Gln Val 
        515                 520                 525             


Glu Glu Gln Leu Glu Trp Leu Ser Gln Arg Ala Asn Ala Leu Leu Ala 
    530                 535                 540                 


Asn Gly Met Asp Leu Lys Asp Asn Gln Leu Val Val Pro Ala Asp Gly 
545                 550                 555                 560 


Leu Tyr Leu Val Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
                565                 570                 575     


Asp Tyr Val Leu Leu Thr His Thr Val Ser Arg Phe Ala Ile Ser Tyr 
            580                 585                 590         


Gln Glu Lys Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys 
        595                 600                 605             


Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro Ile Tyr 
    610                 615                 620                 


Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Gln Leu Ser Ala Glu 
625                 630                 635                 640 


Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr 
                645                 650                 655     


Phe Gly Val Ile Ala Leu 
            660         


<210>  22
<211>  665
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  h83564, mutein (T2R/F63P) of fusion protein 75808

<400>  22

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Arg Ser Ser Ser Arg 
                165                 170                 175     


Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro Gln Ala 
            180                 185                 190         


Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala 
        195                 200                 205             


Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly 
    210                 215                 220                 


Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
225                 230                 235                 240 


Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala Val Ser 
                245                 250                 255     


Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln 
            260                 265                 270         


Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile 
        275                 280                 285             


Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala 
    290                 295                 300                 


Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val 
305                 310                 315                 320 


Tyr Phe Gly Ile Ile Ala Leu Gly Gly Gly Ser Gly Gly Gly Ser Gly 
                325                 330                 335     


Gly Gly Ser Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val 
            340                 345                 350         


Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu 
        355                 360                 365             


Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp 
    370                 375                 380                 


Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln 
385                 390                 395                 400 


Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr 
                405                 410                 415     


His Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu 
            420                 425                 430         


Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala 
        435                 440                 445             


Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln 
    450                 455                 460                 


Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr 
465                 470                 475                 480 


Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
                485                 490                 495     


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Val Arg Ser Ser 
            500                 505                 510         


Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro 
        515                 520                 525             


Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu 
    530                 535                 540                 


Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser 
545                 550                 555                 560 


Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly 
                565                 570                 575     


Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala 
            580                 585                 590         


Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro 
        595                 600                 605             


Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu 
    610                 615                 620                 


Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu 
625                 630                 635                 640 


Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly 
                645                 650                 655     


Gln Val Tyr Phe Gly Ile Ile Ala Leu 
            660                 665 


<210>  23
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-ED-B Affilin, mutein (T2V) of 54646

<400>  23

Met Val Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  24
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-ED-B Affilin, mutein (T2R/F63P) of 54646

<400>  24

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  25
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence of TNFalpha


<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  Thr

<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  Val

<220>
<221>  VARIANT
<222>  (132)..(132)
<223>  Leu

<220>
<221>  VARIANT
<222>  (132)..(132)
<223>  Arg

<400>  25

Met Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Ala His 
1               5                   10                  15      


Val Val Ala Asn His Gln Ala Glu Glu Gln Leu Glu Trp Leu Ser Gln 
            20                  25                  30          


Arg Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn Gln 
        35                  40                  45              


Leu Val Val Pro Ala Asp Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu 
    50                  55                  60                  


Phe Lys Gly Gln Gly Cys Pro Ser Asp Tyr Val Leu Leu Thr His Thr 
65                  70                  75                  80  


Val Ser Arg Phe Ala Ile Ser Tyr Gln Glu Lys Val Asn Leu Leu Ser 
                85                  90                  95      


Ala Ile Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu 
            100                 105                 110         


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
        115                 120                 125             


Lys Gly Asp Gln Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp 
    130                 135                 140                 


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu 
145                 150                 155             


<210>  26
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  26

Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser 
    


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  27

Ser Gly Gly Gly Gly Gly Ser 
1               5           


<210>  28
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  28

Gly Gly Gly Gly Ser 
1               5   


<210>  29
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  29

Ser Ser Ser Ser Gly Ser Ser Ser Ser Gly Ser Ser Ser Ser Gly 
1               5                   10                  15  


