                         SEQUENCE LISTING

<110>  LanzaTech New Zealand Limited
 
<120>  Recombinant microorganisms and uses therefor

<130>  LT81

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  479
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  1
aatatgatat ttatgtccat tgtgaaaggg attatattca actattattc cagttacgtt       60

catagaaatt ttcctttcta aaatatttta ttccatgtca agaactctgt ttatttcatt      120

aaagaactat aagtacaaag tataaggcat ttgaaaaaat aggctagtat attgattgat      180

tatttatttt aaaatgccta agtgaaatat atacatatta taacaataaa ataagtatta      240

gtgtaggatt tttaaataga gtatctattt tcagattaaa tttttgatta tttgatttac      300

attatataat attgagtaaa gtattgacta gcaaaatttt ttgatacttt aatttgtgaa      360

atttcttatc aaaagttata tttttgaata atttttattg aaaaatacaa ctaaaaagga      420

ttatagtata agtgtgtgta attttgtgtt aaatttaaag ggaggaaatg aacatgaaa       479


<210>  2
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Ppta-ack-NotI-F

<400>  2
gagcggccgc aatatgatat ttatgtcc                                          28


<210>  3
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Ppta-ack-NdeI-R

<400>  3
ttccatatgt ttcatgttca tttcctcc                                          28


<210>  4
<211>  2951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised pddABC operon

<400>  4
catatgagaa gtaaaagatt tgaggcatta gcaaaaagac cagttaatca agatggattt       60

gtaaaagaat ggattgaaga aggtttcata gcaatggaat ctccaaatga tccaaaacca      120

agtattaaaa ttgtaaatgg tgctgtaaca gaacttgatg gaaaacctgt atctgatttt      180

gacttaatag atcattttat agcaagatat ggaataaact taaatagagc tgaagaagtt      240

atggctatgg atagtgttaa acttgctaat atgctttgtg atcctaatgt aaaaagatct      300

gagattgtac ctttaactac agcaatgact cctgctaaga tagtagaagt tgtatcacac      360

atgaatgtag tagagatgat gatggcaatg cagaaaatga gagctagaag aactccatca      420

caacaagcac atgtaactaa cgttaaagat aacccagtac aaatagcagc agatgctgca      480

gaaggtgctt ggagaggttt tgacgaacaa gaaactacag ttgcagttgc aagatatgct      540

ccttttaatg ctattgctct tttagttgga tcacaggttg gtagaccagg tgtacttaca      600

caatgtagtt tagaagaagc tactgagctt aaattaggaa tgcttggaca tacatgttat      660

gcagaaacta taagtgttta cggaacagaa cctgttttta cagatggtga tgatactcca      720

tggtctaagg gatttcttgc atcatcatac gcatcaagag gtttaaagat gagatttact      780

tctggatctg gatctgaagt tcagatggga tatgctgaag gtaagagtat gttatatctt      840

gaagctagat gtatatatat aacaaaagct gcaggtgttc aaggattaca aaacggatca      900

gtatcatgca taggtgtacc aagtgcagtt ccatcaggaa ttagagctgt tcttgctgaa      960

aacttaatat gtagttcatt agatttagag tgcgcaagtt ctaatgatca gacatttact     1020

cattcagata tgagaagaac agctagatta cttatgcagt ttcttcctgg aactgatttc     1080

ataagttctg gatattcagc tgtacctaat tacgataata tgtttgctgg aagtaatgaa     1140

gatgctgaag actttgatga ttataatgta atacaaagag acttaaaggt agacggtggt     1200

ttaagaccag taagagaaga agatgtaata gcaataagaa ataaggctgc tagagcactt     1260

caggcagtat tcgcaggtat gggtttacct cctataacag atgaagaagt tgaagcagct     1320

acttatgctc atggttctaa ggatatgcct gaaagaaata ttgttgaaga tataaagttc     1380

gctcaggaaa ttataaataa gaatagaaat ggacttgaag tagttaaagc attagcacag     1440

ggtggtttta ctgatgttgc acaagatatg cttaatattc agaaagctaa attaactggt     1500

gattatttac acacatcagc aataattgtt ggtgacggac aagtattatc agcagtaaac     1560

gatgtaaatg actatgctgg accagctaca ggatatagac ttcaaggtga aagatgggaa     1620

gagataaaga atataccagg tgcacttgat ccaaatgaaa tagattaatt gaacacacaa     1680

aaaaaataat taataaggag gcaaacaaat ggaaataaat gaaaaacttt taagacagat     1740

aattgaagat gtactttcag aaatgaaagg aagtgataaa cctgtatcat ttaatgcacc     1800

tgctgctagt gcagctcctc aagcaactcc tcctgctggt gacggattct taactgaagt     1860

tggtgaagct agacaaggaa cacaacaaga tgaagtaata atagctgttg gaccagcatt     1920

tggattagct cagactgtaa atatagtagg tattccacac aaatcaatac ttagagaagt     1980

tatagctgga atagaagaag aaggtataaa ggcaagagtt attagatgtt ttaagtcttc     2040

tgatgtagca tttgttgctg ttgaaggtaa tagactttct ggatcaggaa taagtattgg     2100

aatacaatca aaaggaacaa cagttattca tcagcaagga ttaccaccac ttagtaacct     2160

tgaacttttt cctcaagcac cattattaac attagagact tatagacaaa taggtaagaa     2220

tgctgctaga tacgcaaaaa gagagtcacc acagccagta ccaactttaa acgatcaaat     2280

ggcaagacct aaatatcagg caaaatctgc aattttacat ataaaagaga ctaaatatgt     2340

agtaacagga aagaatccac aggaacttag agtagcatta taaaaataaa tagtagatat     2400

ataaagtaaa aggaggtaag ataatgaata cagacgcaat agaatcaatg gtaagagatg     2460

tactttcaag aatgaactct ttacaaggag aagctccagc agcagctcca gcagcaggtg     2520

gtgcaagtag aagtgctaga gtttcagatt atccattagc taataagcat cctgaatggg     2580

ttaaaactgc aacaaacaaa actttagatg attttactct tgaaaatgtt ctttcaaata     2640

aagtaactgc tcaggatatg agaattactc cagaaacact tagattacag gctagtatag     2700

ctaaggatgc tggaagagac agattagcta tgaattttga aagagcagct gagttaactg     2760

ctgttcctga tgatagaata ttagaaatat acaatgctct tagaccttat agatctacaa     2820

aagaagaact tttagcaata gcagatgatc ttgagtctag atatcaagca aaaatttgtg     2880

cagcattcgt aagagaagca gctacacttt atgtagagag aaagaaatta aaaggagatg     2940

attaagaatt c                                                          2951


<210>  5
<211>  780
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised budC gene

<400>  5
catatgaaaa aggttgcatt agttacagga gcaggacaag gtataggaaa ggctatagca       60

cttagacttg taaaagatgg atttgcagtt gctattgcag attataacga tactacagct      120

aaagcagttg cttcagaaat taatcaagca ggtggtagag caatggctgt aaaagttgat      180

gttagtgata gagaccaggt ttttgctgct gttgaacaag ctagaaagac attaggtggt      240

tttgatgtaa tagttaataa tgctggtgta gctccatcaa ctccaattga atctataact      300

ccagaaatag tagacaaagt atataatata aacgtaaaag gtgtaatatg gggtatacaa      360

gcagcagtag aagcattcaa gaaagaagga cacggtggta aaataataaa tgcttgttct      420

caggctggtc atgtaggtaa tccagaatta gcagtatact catcatcaaa gtttgcagta      480

agaggtctta cacaaacagc agctagagat cttgctccat taggaattac agtaaatgga      540

tattgccctg gaattgttaa gactcctatg tgggcagaga ttgatagaca agtttctgag      600

gctgcaggta aacctttagg atatggaact gcagagtttg caaagagaat tactcttggt      660

agacttagtg aacctgaaga tgttgcagct tgtgtaagtt atttagcttc acctgatagt      720

gattatatga caggacagtc tcttttaatt gacggtggta tggtatttaa ttaagaattc      780


<210>  6
<211>  2254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised ddrAB operon

<400>  6
gagctcagga ggtttactca tgagatatat agctggaata gatataggaa atagtagtac       60

tgaagtagca ttagcaactt tagatgaagc tggagcatta actataactc atagtgcatt      120

agcagaaact actggaataa aaggaacttt aagaaatgta tttggaatac aagaagcatt      180

agcattagta gcaagaggag ctggaatagc agtaagtgat ataagtttaa taagaataaa      240

tgaagcaact cctgtaatag gagatgtagc aatggaaact ataactgaaa ctataataac      300

tgaaagtact atgataggac ataatcctaa aacacctgga ggagcaggat taggaactgg      360

aataactata acacctcaag aattattaac tagacctgca gatgcacctt atatattagt      420

agtaagtagt gcatttgatt ttgcagatat agcaagtgta ataaatgcaa gtttaagagc      480

aggatatcaa ataactggag taatattaca aagagatgat ggagtattag taagtaatag      540

attagaaaaa cctttaccta tagtagatga agttttatat atagatagaa tacctttagg      600

aatgttagca gcaatagaag tagcagtacc tggaaaagta atagaaactt taagtaatcc      660

ttatggaata gcaactgtat ttaatttaag tcctgaagaa actaaaaata tagtacctat      720

ggcaagagca ttaataggaa atagatctgc agtagtagta aaaactccta gtggagatgt      780

aaaagcaaga gcaatacctg ctggaaattt agaattatta gcacaaggaa gatctgtaag      840

agtagatgta gctgctggag ctgaagcaat aatgaaagca gtagatggat gtggaagatt      900

agataatgta actggagaaa gtggaacaaa tataggagga atgttagaac atgtaagaca      960

aactatggca gaattaacta ataaacctag tagtgaaata tttatacaag atttattagc     1020

agtagatact agtgtacctg taagtgtaac tggaggatta gctggagaat ttagtttaga     1080

acaagcagta ggaatagcaa gtatggtaaa aagtgataga ttacaaatgg caatgatagc     1140

aagagaaata gaacaaaaat taaatataga tgtacaaata ggaggagcag aagcagaagc     1200

agcaatatta ggagcattaa caacacctgg aactactaga cctttagcaa tattagattt     1260

aggagcagga tctactgatg caagtataat aaatcctaaa ggagatataa tagcaactca     1320

tttagctgga gctggagata tggtaactat gataatagct agagaattag gattagaaga     1380

tagatattta gcagaagaaa taaaaaaata tcctttagca aaagtagaaa gtttatttca     1440

tttaagacat gaagatggaa gtgtacaatt ttttagtact cctttacctc ctgcagtatt     1500

tgcaagagta tgtgtagtaa aagcagatga attagtacct ttacctggag atttagcatt     1560

agaaaaagta agagctataa gaagatctgc aaaagaaaga gtatttgtaa ctaatgcatt     1620

aagagcttta agacaagtaa gtcctactgg aaatataaga gatatacctt ttgtagtatt     1680

agttggagga agtagtttag attttgaagt acctcaatta gtaactgatg cattagcaca     1740

ttatagatta gtagctggaa gaggaaatat aagaggatct gaaggaccta gaaatgcagt     1800

agcaacagga ttaatattaa gttggcataa agaatttgca catgaaagat aacaatgtag     1860

gaggttaact atgaatggaa atcatagtgc accagcaata gcaatagcag taatagatgg     1920

atgtgatgga ttatggagag aagtattatt aggaatagaa gaagaaggaa taccttttag     1980

attacaacat catcctgctg gagaagtagt agatagtgca tggcaagcag caagaagtag     2040

tcctttatta gtaggaatag catgtgatag acatatgtta gtagtacatt ataaaaattt     2100

acctgcaagt gcacctttat ttactttaat gcatcatcaa gatagtcaag cacatagaaa     2160

tactggaaat aatgcagcaa gattagtaaa aggaatacca tttagagatt taaatagtga     2220

agcaactgga gaacaacaag atgaataagg tacc                                 2254


<210>  7
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide als-NdeI-F

<400>  7
ggaagtttca tatgaataga gatat                                             25


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide als-EcoR1-R

<400>  8
ggaagtttca tatgaataga gatat                                             25


<210>  9
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide als-NdeI-F

<400>  9
gcgaattcga catagaggtg aatgtaatat gg                                     32


<210>  10
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide als-EcoR1-R

<400>  10
gcgagctctt atttttcaac acttgttatc tca                                    33


<210>  11
<211>  9052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pMTL007C-E5-2,3bdh-468!469s

<400>  11
cctgcagggt gtagtagcct gtgaaataag taaggaaaaa aaagaagtaa gtgttatata       60

tgatgattat tttgtagatg tagataggat aatagaatcc atagaaaata taggttatac      120

agttatataa aaattacttt aaaaattaat aaaaacatgg taaaatataa atcgtataaa      180

gttgtgtaat ttttaagcta aaaaagctta taattatcct tagcactcgt tgaggtgcgc      240

ccagataggg tgttaagtca agtagtttaa ggtactactc tgtaagataa cacagaaaac      300

agccaaccta accgaaaagc gaaagctgat acgggaacag agcacggttg gaaagcgatg      360

agttacctaa agacaatcgg gtacgactga gtcgcaatgt taatcagata taaggtataa      420

gttgtgttta ctgaacgcaa gtttctaatt tcgattagtg ctcgatagag gaaagtgtct      480

gaaacctcta gtacaaagaa aggtaagtta ggctcaacga cttatctgtt atcaccacat      540

ttgtacaatc tggtacaatc tgtaggagaa cctatgggaa cgaaacgaaa gcgatgccga      600

gaatctgaat ttaccaagac ttaacactaa ctggggatac cctaaacaag aatgcctaat      660

agaaaggagg aaaaaggcta tagcactaga gcttgaaaat cttgcaaggg tacggagtac      720

tcgtagtagt ctgagaaggg taacgccctt tacatggcaa aggggtacag ttattgtgta      780

ctaaaattaa aaattgatta gggaggaaaa cctcaaaatg aaaccaacaa tggcaatttt      840

agaaagaatc agtaaaaatt cacaagaaaa tatagacgaa gtttttacaa gactttatcg      900

ttatctttta cgtccagata tttattacgt ggcgacgcgt gaagttccta tactttctag      960

agaataggaa cttcgcgact catagaatta tttcctcccg ttaaataata gataactatt     1020

aaaaatagac aatacttgct cataagtaac ggtacttaaa ttgtttactt tggcgtgttt     1080

cattgcttga tgaaactgat ttttagtaaa cagttgacga tattctcgat tgacccattt     1140

tgaaacaaag tacgtatata gcttccaata tttatctgga acatctgtgg tatggcgggt     1200

aagttttatt aagacactgt ttacttttgg tttaggatga aagcattccg ctggcagctt     1260

aagcaattgc tgaatcgaga cttgagtgtg caagagcaac cctagtgttc ggtgaatatc     1320

caaggtacgc ttgtagaatc cttcttcaac aatcagatag atgtcagacg catggctttc     1380

aaaaaccact tttttaataa tttgtgtgct taaatggtaa ggaatactcc caacaatttt     1440

atacctctgt ttgttaggga attgaaactg tagaatatct tggtgaatta aagtgacacg     1500

agtattcagt tttaattttt ctgacgataa gttgaataga tgactgtcta attcaataga     1560

cgttacctgt ttacttattt tagccagttt cgtcgttaaa tgccctttac ctgttccaat     1620

ttcgtaaacg gtatcggttt cttttaaatt caattgtttt attatttggt tgagtacttt     1680

ttcactcgtt aaaaagtttt gagaatattt tatatttttg ttcataccag caccagaagc     1740

accagcatct cttgggttaa ttgaggcctg agtataaggt gacttatact tgtaatctat     1800

ctaaacgggg aacctctcta gtagacaatc ccgtgctaaa ttgtaggact gccctttaat     1860

aaatacttct atatttaaag aggtatttat gaaaagcgga atttatcaga ttaaaaatac     1920

tttctctaga gaaaatttcg tctggattag ttacttatcg tgtaaaatct gataaatgga     1980

attggttcta cataaatgcc taacgactat ccctttgggg agtagggtca agtgactcga     2040

aacgatagac aacttgcttt aacaagttgg agatatagtc tgctctgcat ggtgacatgc     2100

agctggatat aattccgggg taagattaac gaccttatct gaacataatg ccatatgaat     2160

ccctcctaat ttatacgttt tctctaacaa cttaattata cccactatta ttatttttat     2220

caatatagaa gttcctatac tttctagaga ataggaactt cacgcgttgg gaaatggcaa     2280

tgatagcgaa acaacgtaaa actcttgttg tatgctttca ttgtcatcgt cacgtgattc     2340

ataaacacaa gtgaatgtcg acagtgaatt tttacgaacg aacaataaca gagccgtata     2400

ctccgagagg ggtacgtacg gttcccgaag agggtggtgc aaaccagtca cagtaatgtg     2460

aacaaggcgg tacctcccta cttcaccata tcattttctg cagcccccta gaaataattt     2520

tgtttaactt taagaaggag atatacatat atggctagat cgtccattcc gacagcatcg     2580

ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcactcgta     2640

gtagtctgag aagggtaacg ccctttacat ggcaaagggg tacagttatt gtgtactaaa     2700

attaaaaatt gattagggag gaaaacctca aaatgaaacc aacaatggca attttagaaa     2760

gaatcagtaa aaattcacaa gaaaatatag acgaagtttt tacaagactt tatcgttatc     2820

ttttacgtcc agatatttat tacgtggcgt atcaaaattt atattccaat aaaggagctt     2880

ccacaaaagg aatattagat gatacagcgg atggctttag tgaagaaaaa ataaaaaaga     2940

ttattcaatc tttaaaagac ggaacttact atcctcaacc tgtacgaaga atgtatattg     3000

caaaaaagaa ttctaaaaag atgagacctt taggaattcc aactttcaca gataaattga     3060

tccaagaagc tgtgagaata attcttgaat ctatctatga accggtattc gaagatgtgt     3120

ctcacggttt tagacctcaa cgaagctgtc acacagcttt gaaaacaatc aaaagagagt     3180

ttggcggcgc aagatggttt gtggagggag atataaaagg ctgcttcgat aatatagacc     3240

acgttacact cattggactc atcaatctta aaatcaaaga tatgaaaatg agccaattga     3300

tttataaatt tctaaaagca ggttatctgg aaaactggca gtatcacaaa acttacagcg     3360

gaacacctca aggtggaatt ctatctcctc ttttggccaa catctatctt catgaattgg     3420

ataagtttgt tttacaactc aaaatgaagt ttgaccgaga aagtccagaa agaataacac     3480

ctgaatatcg ggagctccac aatgagataa aaagaatttc tcaccgtctc aagaagttgg     3540

agggtgaaga aaaagctaaa gttcttttag aatatcaaga aaaacgtaaa agattaccca     3600

cactcccctg tacctcacag acaaataaag tattgaaata cgtccggtat gcggacgact     3660

tcattatctc tgttaaagga agcaaagagg actgtcaatg gataaaagaa caattaaaac     3720

tttttattca taacaagcta aaaatggaat tgagtgaaga aaaaacactc atcacacata     3780

gcagtcaacc cgctcgtttt ctgggatatg atatacgagt aaggagatct ggaacgataa     3840

aacgatctgg taaagtcaaa aagagaacac tcaatgggag tgtagaactc cttattcctc     3900

ttcaagacaa aattcgtcaa tttatttttg acaagaaaat agctatccaa aagaaagata     3960

gctcatggtt tccagttcac aggaaatatc ttattcgttc aacagactta gaaatcatca     4020

caatttataa ttctgaactc cgcgggattt gtaattacta cggtctagca agtaatttta     4080

accagctcaa ttattttgct tatcttatgg aatacagctg tctaaaaacg atagcctcca     4140

aacataaggg aacactttca aaaaccattt ccatgtttaa agatggaagt ggttcgtggg     4200

ggatcccgta tgagataaag caaggtaagc agcgccgtta ttttgcaaat tttagtgaat     4260

gtaaatcccc ttatcaattt acggatgaga taagtcaagc tcctgtattg tatggctatg     4320

cccggaatac tcttgaaaac aggttaaaag ctaaatgttg tgaattatgt gggacgtctg     4380

atgaaaatac ttcctatgaa attcaccatg tcaataaggt caaaaatctt aaaggcaaag     4440

aaaaatggga aatggcaatg atagcgaaac aacgtaaaac tcttgttgta tgctttcatt     4500

gtcatcgtca cgtgattcat aaacacaagt gaatgtcgag cacccgttct cggagcactg     4560

tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac     4620

tacgcgatca tggcgaccac acccgtcctg tggatcgcca agccgccgat ggtagtgtgg     4680

ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg     4740

aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca     4800

aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga     4860

cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt     4920

tttgcgtttc tacaaactct tcctgtcgtc atatctacaa gccatccccc cacagatacg     4980

ggcgcgccgc cattattttt ttgaacaatt gacaattcat ttcttatttt ttattaagtg     5040

atagtcaaaa ggcataacag tgctgaatag aaagaaattt acagaaaaga aaattataga     5100

atttagtatg attaattata ctcatttatg aatgtttaat tgaatacaaa aaaaaatact     5160

tgttatgtat tcaattacgg gttaaaatat agacaagttg aaaaatttaa taaaaaaata     5220

agtcctcagc tcttatatat taagctacca acttagtata taagccaaaa cttaaatgtg     5280

ctaccaacac atcaagccgt tagagaactc tatctatagc aatatttcaa atgtaccgac     5340

atacaagaga aacattaact atatatattc aatttatgag attatcttaa cagatataaa     5400

tgtaaattgc aataagtaag atttagaagt ttatagcctt tgtgtattgg aagcagtacg     5460

caaaggcttt tttatttgat aaaaattaga agtatattta ttttttcata attaatttat     5520

gaaaatgaaa gggggtgagc aaagtgacag aggaaagcag tatcttatca aataacaagg     5580

tattagcaat atcattattg actttagcag taaacattat gacttttata gtgcttgtag     5640

ctaagtagta cgaaaggggg agctttaaaa agctccttgg aatacataga attcataaat     5700

taatttatga aaagaagggc gtatatgaaa acttgtaaaa attgcaaaga gtttattaaa     5760

gatactgaaa tatgcaaaat acattcgttg atgattcatg ataaaacagt agcaacctat     5820

tgcagtaaat acaatgagtc aagatgttta cataaaggga aagtccaatg tattaattgt     5880

tcaaagatga accgatatgg atggtgtgcc ataaaaatga gatgttttac agaggaagaa     5940

cagaaaaaag aacgtacatg cattaaatat tatgcaagga gctttaaaaa agctcatgta     6000

aagaagagta aaaagaaaaa ataatttatt tattaattta atattgagag tgccgacaca     6060

gtatgcacta aaaaatatat ctgtggtgta gtgagccgat acaaaaggat agtcactcgc     6120

attttcataa tacatcttat gttatgatta tgtgtcggtg ggacttcacg acgaaaaccc     6180

acaataaaaa aagagttcgg ggtagggtta agcatagttg aggcaactaa acaatcaagc     6240

taggatatgc agtagcagac cgtaaggtcg ttgtttaggt gtgttgtaat acatacgcta     6300

ttaagatgta aaaatacgga taccaatgaa gggaaaagta taatttttgg atgtagtttg     6360

tttgttcatc tatgggcaaa ctacgtccaa agccgtttcc aaatctgcta aaaagtatat     6420

cctttctaaa atcaaagtca agtatgaaat cataaataaa gtttaatttt gaagttatta     6480

tgatattatg tttttctatt aaaataaatt aagtatatag aatagtttaa taatagtata     6540

tacttaatgt gataagtgtc tgacagtgtc acagaaagga tgattgttat ggattataag     6600

cggccggcca gtgggcaagt tgaaaaattc acaaaaatgt ggtataatat ctttgttcat     6660

tagagcgata aacttgaatt tgagagggaa cttagatggt atttgaaaaa attgataaaa     6720

atagttggaa cagaaaagag tattttgacc actactttgc aagtgtacct tgtacctaca     6780

gcatgaccgt taaagtggat atcacacaaa taaaggaaaa gggaatgaaa ctatatcctg     6840

caatgcttta ttatattgca atgattgtaa accgccattc agagtttagg acggcaatca     6900

atcaagatgg tgaattgggg atatatgatg agatgatacc aagctataca atatttcaca     6960

atgatactga aacattttcc agcctttgga ctgagtgtaa gtctgacttt aaatcatttt     7020

tagcagatta tgaaagtgat acgcaacggt atggaaacaa tcatagaatg gaaggaaagc     7080

caaatgctcc ggaaaacatt tttaatgtat ctatgatacc gtggtcaacc ttcgatggct     7140

ttaatctgaa tttgcagaaa ggatatgatt atttgattcc tatttttact atggggaaat     7200

attataaaga agataacaaa attatacttc ctttggcaat tcaagttcat cacgcagtat     7260

gtgacggatt tcacatttgc cgttttgtaa acgaattgca ggaattgata aatagttaac     7320

ttcaggtttg tctgtaacta aaaacaagta tttaagcaaa aacatcgtag aaatacggtg     7380

ttttttgtta ccctaagttt aaactccttt ttgataatct catgaccaaa atcccttaac     7440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     7500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     7560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     7620

gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga     7680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     7740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     7800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     7860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     7920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     7980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     8040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     8100

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     8160

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     8220

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca     8280

gggccccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac     8340

gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc     8400

agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc     8460

cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg     8520

ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca     8580

gcccacctat caaggtgtac tgccttccag acgaacgaag agcgattgag gaaaaggcgg     8640

cggcggccgg catgagcctg tcggcctacc tgctggccgt cggccagggc tacaaaatca     8700

cgggcgtcgt ggactatgag cacgtccgcg agctggcccg catcaatggc gacctgggcc     8760

gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg cggttcggtg     8820

atgccacgat cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag cttggcaagg     8880

tcatgatggg cgtggtccgc ccgagggcag agccatgact tttttagccg ctaaaacggc     8940

cggggggtgc gcgtgattgc caagcacgtc cccatgcgct ccatcaagaa gagcgacttc     9000

gcggagctgg tgaagtacat caccgacgag caaggcaaga ccgatcgggc cc             9052


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og42f

<400>  12
ctgcacctaa aaccaaagca gtatt                                             25


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og43r

<400>  13
atcctttaag caagagtact gcacc                                             25


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide fD1

<400>  14
ctgcacctaa aaccaaagca gtatt                                             25


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide rP2

<400>  15
atcctttaag caagagtact gcacc                                             25


<210>  16
<211>  1160
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  16
agtggcactg gaaaagaact cttagctcaa tctattcaca attatagtga aagatgtgaa       60

ggcccttttg tagctataaa ttgtagttct atacctagag aacttgtaga aagtgagctt      120

tttggttatg aaaaaggagc ttttacggga gctttaaagc aaggaaagcc tggaaagttt      180

gaattagcag atggaggaac tatttttttg gatgaagtag gagagcttcc tcttgatata      240

cagtcaaagc ttttaagggt tcttgataat aataaaatta caagagttgg aggaacttat      300

gaaaaacagc taaatgtaag gataatagga gctacaaaca gggtgctcaa ggatgaaatt      360

aaaaagaaaa atttcagaag tgacctttat tatagattga gtgtgatgaa tataaaaact      420

gtcccactta gggaaagaaa agaagatata gagcttttaa ttaaatattt tatggaagaa      480

ttgaattcta aaagtttgtg taagaagaaa gtagtggaaa aagcatacat agaaaagatt      540

aaagcttatg attggcctgg aaatgttaga gaacttagaa atgtaataga gagggattac      600

tatttaagtg aggataagat ggcccctttg gattatttag aaaaagaagt ttatgaaaaa      660

aatgtctcct ctgatccagt aaatattagt gtgcttccaa tggatgtttt agaaaaagaa      720

aacattgaaa atgcacttaa aaagtgtaag ggaaatatat taaaagctgc aaaatcttta      780

aatatcagta gatctaccat gtatagaaaa atgaaaaagt atggaataaa aagtgtgtca      840

aaatgaccag aaaagagtaa gattctcaaa ataggacact aagtatgtgt cataatggca      900

catagtgatt ttaaatgtct ttttaacagg tttcttgttt ttggtatggc ttttgcttat      960

aaaatatagt gaatatatta acaggtatat gtaaatttta atattgccat actattataa     1020

aaaaggagag ataattatga aagctgtatt gtggtatgat aaaaaagatg taagagtaga     1080

ggaaattgag gaacctaagg taaaagaaaa tgctgtaaaa attaaagtga aatggtgtgg     1140

tatatgtggt tctgacttgc                                                 1160


<210>  17
<211>  834
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  17
tattgaggag gccaaaaatg agctttaaga aaaatgtata cgatacaatg agggaactaa       60

tatctgtgcc aagcatatct ggtacaaaag aagagtgtgc ggcagcagaa aaaatatatg      120

aaaaaatttt ggaaatacct tattttaagg acaatcctga aaatctagga atagagcaaa      180

ttgaagatga tcctttagga agaagctttg tatgggcagt agtaaatgga aatgaaaatt      240

caccaaattc gtttatactt tcaggtcatt tggatgtagt tggagtagaa gaatttggac      300

atttaaaatc tatggctttt gatgtagatg aatgtactaa aagaatctca gaattgaatt      360

tagatgaaga tgctatggag gattttaaat caggagattg gatatttgga aggggaactg      420

cagacatgaa gtttggagtg gccctcaata tggaactttt aagagaattc agtaaagaga      480

gaaactttaa gggaaactta ttacttttag tagttcctgg tgaagagagt aattccgaag      540

gaatgattgc tgcagctcca tttcttctta aattaaagga agagaggaag tacaattact      600

gtggtatgat aatatcagag ccaagtatac ctgaaagagg agaaaaagaa ggcaagagat      660

tatatatagg tagtgtaggt aaaattatgc ctttattttt ttgtgtggga aaagaaactc      720

atgtagggga atctttaaga ggattgaatc caaatttgct agtttcagag ataaacaaat      780

taatggaatg taatccagat ctctcagata gcgtttatga tactgtgact ccac            834


<210>  18
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og13f

<400>  18
attcatcctg caggagtggc actggaaaag aactcttag                              39


<210>  19
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og14r

<400>  19
gactgcggcc gcgcaagtca gaaccacata taccaca                                37


<210>  20
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og15f

<400>  20
atatgctagc tattgaggag gccaaaaatg agctt                                  35


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og16r

<400>  21
gactggcgcg ccgtggagtc acagtatcat aaacgct                                37


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og33f

<400>  22
aatggcaggg cagataattg taatg                                             25


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Og34r

<400>  23
aaggcattct gagccagttc tttta                                             25


<210>  24
<211>  1062
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  24
acagttaaaa agcatatcta acagtccttc cactgtacta attcaaggcg aaagcggtac       60

aggtaaagaa cttattgcgc agtccatcca caatgacagc agcagaaaaa ataacagctt      120

tatagcaata aattgcggtg ccatacccaa aaatttaata gaaagtgaat tattcggata      180

tgaagatgga tcattcacag gtgcaaaaca tggagggcgt gcaggaaaat ttgaacttgc      240

aaatggtggt actttatttt tagatgaaat tggggaaatg cctttagata tgcaagtaaa      300

tcttttaaga gttctccaag aaaactgtat tacaagaata ggcgggaaca gatgtgtaaa      360

aatagatata agaatcattg cagctactaa taaaaatttg agggaagaaa tacataaagg      420

aacttttcgc gaagatttat actatagact aaatgtaata cctatatatg taccaccact      480

gcgggaaaga gatatggata ttaaaatact gataaactat tttttaaaga taaaagcttt      540

taaacttaaa aaacctattc caatagtaag acctgatata tatcaaaagc tcttaaatta      600

taattggccc ggaaatgtaa gagaattgga aaattgtatt gaaaatatcg taaatatgaa      660

tggaaataca tctttcaact tcgaaaatag tatttcagta aatacgcaaa ctagtccttg      720

tactacaaaa tttaaatatg atatgtattc attaaaagag ttggaaaaag aagcaataac      780

aaattgtatg agtaattgca atggtaacat tgcaaaagct tctaaaattc tgggaataaa      840

tagaagtact ttgtatacaa aaataaaaaa atatcaaatt aatttttctt aaagtgtatg      900

taaacacaac tttgttgtaa aaagcaacat tattttctta aaaaatgttg ctttttacag      960

catttttcaa ttatatatat taaccttata aagtcctacc cccctaaatt caaccttttc     1020

atgataaaaa acatactggc acaacatttg cttatatatt ta                        1062


<210>  25
<211>  823
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  25
cgtattttta attgcgaact taagatttaa ttaatatcta ctatgagtaa gtcaacatat       60

atacctaaat tatgataaaa ttatatatta taatttcaaa ataaacataa ctataataat      120

acactaagat aaagctattt atctgatggc tacctactgt aacactccct cttctatcaa      180

agtgagagat aacagtagct acgcccctag ataattcatc taaacttagt gggagaaaca      240

aaactctaaa gagaaagcga ttcactttaa atcaaagatt tgagatatct gcttctccca      300

ctaagtaaga ttcattgata taaaaaggaa ggtaatctaa taatgtttaa accatttact      360

catagtgaaa tagtcagtag gtctcttaat agatgcatta aataccatat agaaaaaggt      420

ataccaaaac ctaaacgaac acttagtcgc aaagaattgg acaacttaat aaaagaaaac      480

aacgatatta taaaaatagc aaaaccattt atggaaatac tttatgattt tttaagtgga      540

tcaggtttct cattatatct cacagacaaa aatggaattg tattaactat cataggtgac      600

aaagatattg taatggagca ggcaaaggct ggaatagcag aaggtattga tctgagtgaa      660

caaagtgcag gtacaaatgc agcaggaact gctatttttg aaaatttgtc agttcaactt      720

tcaggcaaag aacattttat aaatactttt cagatttata cctgctctgc atctgtcata      780

cataacgaac aaggaaatat aatcggatgt ctaactttaa ctg                        823


<210>  26
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Sec5f

<400>  26
attcatcctg caggacagtt aaaaagcata tctaacagt                              39


<210>  27
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Sec5r

<400>  27
gactgcggcc gctaaatata taagcaaatg ttgtgcc                                37


<210>  28
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Sec3f

<400>  28
atatgctagc gtatttttaa ttgcgaactt aaga                                   34


<210>  29
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Sec3r

<400>  29
gactggcgcg ccagttaaag ttagacatcc gattat                                 36


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide SecOf

<400>  30
ttggaatttt agctgtagat aacaa                                             25


<210>  31
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide SecOr

<400>  31
taagtgattt tcaatggact ttact                                             25


<210>  32
<211>  344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  intron target region against alcohol dehydrogenase

<400>  32
aagcttataa ttatccttag atatcaatct tgtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg attatatctc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttagca agattgactt atctgttatc accacatttg taca                       344


<210>  33
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide of SecCTf

<400>  33
tgattttagg ccatgaagct gtagg                                             25


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide of SecCTr

<400>  34
catgatttgt tcaactatat cacc                                              24


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide GnK-F

<400>  35
tcaggacctt ctggaactgg                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide GnK-R

<400>  36
acctcccctt ttcttggaga                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide FoT4L-F

<400>  37
caggtttcgg tgctgaccta                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide FoT4L-F

<400>  38
aactccgccg ttgtatttca                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide KpBDH -F

<400>  39
aagtttctga ggctgcaggt                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide KpBDH -R

<400>  40
aagtttctga ggctgcaggt                                                   20


