                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  Eukaryotic Transposase Mutants and Transposon End Compositions

<130>   02240-347413 (P11965-02)

<150>  US Provisional  61/652,560
<151>  2012-05-29

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  612
<212>  PRT
<213>  Musca domestica

<400>  1

Met Gln Lys Met Asp Asn Leu Glu Val Lys Ala Lys Ile Asn Gln Gly 
1               5                   10                  15      


Leu Tyr Lys Ile Thr Pro Arg His Lys Gly Thr Ser Phe Ile Trp Asn 
            20                  25                  30          


Val Leu Ala Asp Ile Gln Lys Glu Asp Asp Thr Leu Val Glu Gly Trp 
        35                  40                  45              


Val Phe Cys Arg Lys Cys Glu Lys Val Leu Lys Tyr Thr Thr Arg Gln 
    50                  55                  60                  


Thr Ser Asn Leu Cys Arg His Lys Cys Cys Ala Ser Leu Lys Gln Ser 
65                  70                  75                  80  


Arg Glu Leu Lys Thr Val Ser Ala Asp Cys Lys Lys Glu Ala Ile Glu 
                85                  90                  95      


Lys Cys Ala Gln Trp Val Val Arg Asp Cys Arg Pro Phe Ser Ala Val 
            100                 105                 110         


Ser Gly Ser Gly Phe Ile Asp Met Ile Lys Phe Phe Ile Lys Val Gly 
        115                 120                 125             


Ala Glu Tyr Gly Glu His Val Asn Val Glu Glu Leu Leu Pro Ser Pro 
    130                 135                 140                 


Ile Thr Leu Ser Arg Lys Val Thr Ser Asp Ala Lys Glu Lys Lys Ala 
145                 150                 155                 160 


Leu Ile Ser Arg Glu Ile Lys Ser Ala Val Glu Lys Asp Gly Ala Ser 
                165                 170                 175     


Ala Thr Ile Asp Leu Trp Thr Asp Asn Tyr Ile Lys Arg Asn Phe Leu 
            180                 185                 190         


Gly Val Thr Leu His Tyr His Glu Asn Asn Glu Leu Arg Asp Leu Ile 
        195                 200                 205             


Leu Gly Leu Lys Ser Leu Asp Phe Glu Arg Ser Thr Ala Glu Asn Ile 
    210                 215                 220                 


Tyr Lys Lys Leu Lys Ala Ile Phe Leu Gln Phe Asn Val Glu Asp Leu 
225                 230                 235                 240 


Ser Ser Ile Lys Phe Val Thr Asp Arg Gly Ala Asn Val Val Lys Ser 
                245                 250                 255     


Leu Ala Asn Asn Ile Arg Ile Asn Cys Ser Ser His Leu Leu Ser Asn 
            260                 265                 270         


Val Leu Glu Asn Ser Phe Glu Glu Thr Pro Glu Leu Asn Val Pro Ile 
        275                 280                 285             


Leu Ala Cys Lys Asn Ile Val Lys Tyr Phe Lys Lys Ala Asn Leu Gln 
    290                 295                 300                 


His Arg Leu Arg Ser Ser Leu Lys Ser Glu Cys Pro Thr Arg Trp Asn 
305                 310                 315                 320 


Ser Thr Tyr Thr Met Leu Arg Ser Ile Leu Asp Asn Trp Glu Ser Val 
                325                 330                 335     


Ile Gln Ile Leu Ser Glu Ala Gly Glu Thr Gln Arg Ile Val His Ile 
            340                 345                 350         


Asn Lys Ser Ile Ile Gln Thr Met Val Asn Ile Leu Asp Gly Phe Glu 
        355                 360                 365             


Arg Ile Phe Lys Glu Leu Gln Thr Cys Ser Ser Pro Ser Leu Cys Phe 
    370                 375                 380                 


Val Val Pro Ser Ile Leu Lys Val Lys Glu Ile Cys Ser Pro Asp Val 
385                 390                 395                 400 


Gly Asp Val Ala Asp Ile Ala Lys Leu Lys Val Asn Ile Ile Lys Asn 
                405                 410                 415     


Val Arg Ile Ile Trp Glu Glu Asn Leu Ser Ile Trp His Tyr Thr Ala 
            420                 425                 430         


Phe Phe Phe Tyr Pro Pro Ala Leu His Met Gln Gln Glu Lys Val Ala 
        435                 440                 445             


Gln Ile Lys Glu Phe Cys Leu Ser Lys Met Glu Asp Leu Glu Leu Ile 
    450                 455                 460                 


Asn Arg Met Ser Ser Phe Asn Glu Leu Ser Ala Thr Gln Leu Asn Gln 
465                 470                 475                 480 


Ser Asp Ser Asn Ser His Asn Ser Ile Asp Leu Thr Ser His Ser Lys 
                485                 490                 495     


Asp Ile Ser Thr Thr Ser Phe Phe Phe Pro Gln Leu Thr Gln Asn Asn 
            500                 505                 510         


Ser Arg Glu Pro Pro Val Cys Pro Ser Asp Glu Phe Glu Phe Tyr Arg 
        515                 520                 525             


Lys Glu Ile Val Ile Leu Ser Glu Asp Phe Lys Val Met Glu Trp Trp 
    530                 535                 540                 


Asn Leu Asn Ser Lys Lys Tyr Pro Lys Leu Ser Lys Leu Ala Leu Ser 
545                 550                 555                 560 


Leu Leu Ser Ile Pro Ala Ser Ser Ala Ala Ser Glu Arg Thr Phe Ser 
                565                 570                 575     


Leu Ala Gly Asn Ile Ile Thr Glu Lys Arg Asn Arg Ile Gly Gln Gln 
            580                 585                 590         


Thr Val Asp Ser Leu Leu Phe Leu Asn Ser Phe Tyr Lys Asn Phe Cys 
        595                 600                 605             


Lys Leu Asp Ile 
    610         


<210>  2
<211>  1839
<212>  DNA
<213>  Musca domestica

<400>  2
atgcagaaaa tggacaattt ggaagtgaaa gcaaaaatca accaaggatt atataaaatt       60

actccgcgac ataaaggaac aagttttatt tggaacgttt tagcggatat acagaaagaa      120

gacgatacat tggtggaagg gtgggtgttt tgccgaaaat gcgaaaaagt tttaaaatac      180

acaactaggc agacatcaaa cttatgtcgt cataaatgct gtgcctctct aaagcaatcc      240

cgagaattaa aaactgtttc agctgattgc aaaaaggaag caattgaaaa atgtgcacaa      300

tgggtggtac gagattgtcg gcctttttcg gccgtctctg gatccggctt tatcgatatg      360

ataaaatttt ttattaaagt tggagccgaa tatggtgaac atgtcaacgt tgaggaattg      420

ttaccaagtc caataacgct atcgagaaag gtaacttcgg atgcaaaaga aaaaaaagct      480

ctgattagtc gagaaattaa gtctgctgta gagaaagatg gtgcatcagc aacgatagat      540

ttgtggaccg ataattatat aaaacggaat tttttgggag taacgttaca ctaccatgaa      600

aacaatgaac tgcgagatct aattttaggt ttaaagtcct tagattttga aagatccaca      660

gcagaaaata tttataagaa gcttaaagcc atttttttac aattcaacgt cgaagacttg      720

agtagtataa aatttgtgac agatagagga gccaatgtcg taaaatcatt ggcaaataat      780

atcagaatta actgcagcag ccatttgctt tcaaacgtgt tggaaaattc atttgaggag      840

acacctgaac tcaatgtgcc tattcttgct tgcaaaaata ttgtaaaata tttcaagaaa      900

gccaatctgc agcacagact tcgaagttct ttaaaaagtg agtgccctac acggtggaat      960

tccacataca cgatgcttcg atctattctc gacaactggg aaagcgtgat tcaaatatta     1020

agtgaggcgg gagagacaca gagaattgtt catataaata agtcgataat tcaaacaatg     1080

gtcaacatcc tcgatgggtt tgaaagaatt tttaaagaat tacaaacatg cagttcacca     1140

tctctgtgtt ttgttgtgcc ttccatttta aaagtaaaag aaatatgttc acctgacgtt     1200

ggcgacgttg cagatatagc aaaattgaaa gtgaacatta taaaaaatgt aagaataata     1260

tgggaagaaa atttaagcat atggcactac acagcatttt ttttctatcc gcccgccttg     1320

catatgcaac aagagaaagt ggcacaaatt aaagaatttt gcttatccaa aatggaagat     1380

ttggaattaa taaaccgcat gagttccttt aacgaattat ccgcaactca gcttaaccag     1440

tcggactcca atagccacaa cagtatagat ttaacatccc attcaaaaga catttcaacg     1500

acaagtttct ttttcccgca attaactcag aacaatagtc gtgagccacc agtgtgtcca     1560

agcgatgaat ttgaatttta tcgtaaagaa atagttattt taagcgaaga ttttaaagtt     1620

atggaatggt ggaatcttaa ttcaaaaaag tatcctaaac tatctaaact ggctttgtcg     1680

ttattatcaa tacctgcaag tagcgctgca tcggaaagga cattttccct agctggaaat     1740

ataataactg aaaagagaaa caggattggg caacaaactg tcgacagctt gttattttta     1800

aattcctttt acaaaaattt ttgtaaatta gatatataa                            1839


<210>  3
<211>  17
<212>  DNA
<213>  Musca domestica

<400>  3
cttgttgttg ttctctg                                                      17


<210>  4
<211>  17
<212>  DNA
<213>  Musca domestica

<400>  4
cttgttgaag ttctctg                                                      17


<210>  5
<211>  612
<212>  PRT
<213>  Musca domestica

<400>  5

Met Glu Lys Met Asp Asn Leu Glu Val Lys Ala Lys Ile Asn Gln Gly 
1               5                   10                  15      


Leu Tyr Lys Ile Thr Pro Arg His Lys Gly Thr Ser Phe Ile Trp Asn 
            20                  25                  30          


Val Leu Ala Asp Ile Gln Lys Glu Asp Asp Thr Leu Val Glu Gly Trp 
        35                  40                  45              


Val Phe Cys Arg Lys Cys Glu Lys Val Leu Lys Tyr Thr Thr Arg Gln 
    50                  55                  60                  


Thr Ser Asn Leu Cys Arg His Lys Cys Cys Ala Ser Leu Lys Gln Ser 
65                  70                  75                  80  


Arg Glu Leu Lys Thr Val Ser Ala Asp Cys Lys Lys Glu Ala Ile Glu 
                85                  90                  95      


Lys Cys Ala Gln Trp Val Val Arg Asp Cys Arg Pro Phe Ser Ala Val 
            100                 105                 110         


Ser Gly Ser Gly Phe Ile Asp Met Ile Lys Phe Phe Ile Lys Val Gly 
        115                 120                 125             


Ala Glu Tyr Gly Glu His Val Asn Val Glu Glu Leu Leu Pro Ser Pro 
    130                 135                 140                 


Ile Thr Leu Ser Arg Lys Val Thr Ser Asp Ala Lys Glu Lys Lys Ala 
145                 150                 155                 160 


Leu Ile Ser Arg Glu Ile Lys Ser Ala Val Glu Lys Asp Gly Ala Ser 
                165                 170                 175     


Ala Thr Ile Asp Leu Trp Thr Asp Asn Tyr Ile Lys Arg Asn Phe Leu 
            180                 185                 190         


Gly Val Thr Leu His Tyr His Glu Asn Asn Glu Leu Arg Asp Leu Ile 
        195                 200                 205             


Leu Gly Leu Lys Ser Leu Asp Phe Glu Arg Ser Thr Ala Glu Asn Ile 
    210                 215                 220                 


Tyr Lys Lys Leu Lys Ala Ile Phe Ser Gln Phe Asn Val Glu Asp Leu 
225                 230                 235                 240 


Ser Ser Ile Lys Phe Val Thr Asp Arg Gly Ala Asn Val Val Lys Ser 
                245                 250                 255     


Leu Ala Asn Asn Ile Arg Ile Asn Cys Ser Ser His Leu Leu Ser Asn 
            260                 265                 270         


Val Leu Glu Asn Ser Phe Glu Glu Thr Pro Glu Leu Asn Met Pro Ile 
        275                 280                 285             


Leu Ala Cys Lys Asn Ile Val Lys Tyr Phe Lys Lys Ala Asn Leu Gln 
    290                 295                 300                 


His Arg Leu Arg Ser Ser Leu Lys Ser Glu Cys Pro Thr Arg Trp Asn 
305                 310                 315                 320 


Ser Thr Tyr Thr Met Leu Arg Ser Ile Leu Asp Asn Trp Glu Ser Val 
                325                 330                 335     


Ile Gln Ile Leu Ser Glu Ala Gly Glu Thr Gln Arg Ile Val His Ile 
            340                 345                 350         


Asn Lys Ser Ile Ile Gln Thr Met Val Asn Ile Leu Asp Gly Phe Glu 
        355                 360                 365             


Ala Ile Phe Lys Glu Leu Gln Thr Cys Ser Ser Pro Ser Leu Cys Phe 
    370                 375                 380                 


Val Val Pro Ser Ile Leu Lys Val Lys Glu Ile Cys Ser Pro Asp Val 
385                 390                 395                 400 


Gly Asp Val Ala Asp Ile Ala Lys Leu Lys Val Asn Ile Ile Lys Asn 
                405                 410                 415     


Val Arg Ile Ile Trp Glu Glu Asn Leu Ser Ile Trp His Tyr Thr Ala 
            420                 425                 430         


Phe Phe Phe Tyr Pro Pro Ala Leu His Met Gln Gln Glu Lys Val Ala 
        435                 440                 445             


Gln Ile Lys Glu Phe Cys Leu Ser Lys Met Glu Asp Leu Glu Leu Ile 
    450                 455                 460                 


Asn Arg Met Ser Ser Phe Asn Glu Leu Ser Ala Thr Gln Leu Asn Gln 
465                 470                 475                 480 


Ser Asp Ser Asn Ser His Asn Ser Ile Asp Leu Thr Ser His Ser Lys 
                485                 490                 495     


Asp Ile Ser Thr Thr Ser Ala Ala Phe Pro Gln Leu Thr Gln Asn Asn 
            500                 505                 510         


Ser Arg Glu Pro Pro Val Cys Pro Ser Asp Glu Phe Glu Phe Tyr Arg 
        515                 520                 525             


Lys Glu Ile Val Ile Leu Ser Glu Asp Phe Lys Val Met Glu Trp Trp 
    530                 535                 540                 


Asn Leu Asn Ser Lys Lys Tyr Pro Lys Leu Ser Lys Leu Ala Leu Ser 
545                 550                 555                 560 


Leu Leu Ser Ile Pro Ala Ser Ser Ala Ala Ser Glu Arg Thr Phe Ser 
                565                 570                 575     


Leu Ala Gly Asn Ile Ile Thr Glu Lys Arg Asn Arg Ile Gly Gln Gln 
            580                 585                 590         


Thr Val Asp Ser Leu Leu Phe Leu Asn Ser Phe Tyr Lys Asn Phe Cys 
        595                 600                 605             


Lys Leu Asp Ile 
    610         


<210>  6
<211>  1839
<212>  DNA
<213>  Musca domestica

<400>  6
atggagaaaa tggacaattt ggaagtgaaa gcaaaaatca accaaggatt atataaaatt       60

actccgcgac ataaaggaac aagttttatt tggaacgttt tagcggatat acagaaagaa      120

gacgatacat tggtggaagg gtgggtgttt tgccgaaaat gcgaaaaagt tttaaaatac      180

acaactaggc agacatcaaa cttatgtcgt cataaatgct gtgcctctct aaagcaatcc      240

cgagaattaa aaactgtttc agctgattgc aaaaaggaag caattgaaaa atgtgcacaa      300

tgggtggtac gagattgtcg gcctttttcg gccgtctctg gatccggctt tatcgatatg      360

ataaaatttt ttattaaagt tggagccgaa tatggtgaac atgtcaacgt tgaggaattg      420

ttaccaagtc caataacgct atcgagaaag gtaacttcgg atgcaaaaga aaaaaaagct      480

ctgattagtc gagaaattaa gtctgctgta gagaaagatg gtgcatcagc aacgatagat      540

ttgtggaccg ataattatat aaaacggaat tttttgggag taacgttaca ctaccatgaa      600

aacaatgaac tgcgagatct aattttaggt ttaaagtcct tagattttga aagatccaca      660

gcagaaaata tttataagaa gcttaaagcc attttttcac aattcaacgt cgaagacttg      720

agtagtataa aatttgtgac agatagagga gccaatgtcg taaaatcatt ggcaaataat      780

atcagaatta actgcagcag ccatttgctt tcaaacgtgt tggaaaattc atttgaggag      840

acacctgaac tcaatatgcc tattcttgct tgcaaaaata ttgtaaaata tttcaagaaa      900

gccaatctgc agcacagact tcgaagttct ttaaaaagtg agtgccctac acggtggaat      960

tccacataca cgatgcttcg atctattctc gacaactggg aaagcgtgat tcaaatatta     1020

agtgaggcgg gagagacaca gagaattgtt catataaata agtcgataat tcaaacaatg     1080

gtcaacatcc tcgatgggtt tgaagcaatt tttaaagaat tacaaacatg cagttcacca     1140

tctctgtgtt ttgttgtgcc ttccatttta aaagtaaaag aaatatgttc acctgacgtt     1200

ggcgacgttg cagatatagc aaaattgaaa gtgaacatta taaaaaatgt aagaataata     1260

tgggaagaaa atttaagcat atggcactac acagcatttt ttttctatcc gcccgccttg     1320

catatgcaac aagagaaagt ggcacaaatt aaagaatttt gcttatccaa aatggaagat     1380

ttggaattaa taaaccgcat gagttccttt aacgaattat ccgcaactca gcttaaccag     1440

tcggactcca atagccacaa cagtatagat ttaacatccc attcaaaaga catttcaacg     1500

acaagtgccg ctttcccgca attaactcag aacaatagtc gtgagccacc agtgtgtcca     1560

agcgatgaat ttgaatttta tcgtaaagaa atagttattt taagcgaaga ttttaaagtt     1620

atggaatggt ggaatcttaa ttcaaaaaag tatcctaaac tatctaaact ggctttgtcg     1680

ttattatcaa tacctgcaag tagcgctgca tcggaaagga cattttccct agctggaaat     1740

ataataactg aaaagagaaa caggattggg caacaaactg tcgacagctt gttattttta     1800

aattcctttt acaaaaattt ttgtaaatta gatatatag                            1839


<210>  7
<211>  592
<212>  PRT
<213>  Musca domestica

<400>  7

Met Glu Lys Met Asp Asn Leu Glu Val Lys Ala Lys Ile Asn Gln Gly 
1               5                   10                  15      


Leu Tyr Lys Ile Thr Pro Arg His Lys Gly Thr Ser Phe Ile Trp Asn 
            20                  25                  30          


Val Leu Ala Asp Ile Gln Lys Glu Asp Asp Thr Leu Val Glu Gly Trp 
        35                  40                  45              


Val Phe Cys Arg Lys Cys Glu Lys Val Leu Lys Tyr Thr Thr Arg Gln 
    50                  55                  60                  


Thr Ser Asn Leu Cys Arg His Lys Cys Cys Ala Ser Leu Lys Gln Ser 
65                  70                  75                  80  


Arg Glu Leu Lys Thr Val Ser Ala Asp Cys Lys Lys Glu Ala Ile Glu 
                85                  90                  95      


Lys Cys Ala Gln Trp Val Val Arg Asp Cys Arg Pro Phe Ser Ala Val 
            100                 105                 110         


Ser Gly Ser Gly Phe Ile Asp Met Ile Lys Phe Phe Ile Lys Val Gly 
        115                 120                 125             


Ala Glu Tyr Gly Glu His Val Asn Val Glu Glu Leu Leu Pro Ser Pro 
    130                 135                 140                 


Ile Thr Leu Ser Arg Lys Val Thr Ser Asp Ala Lys Glu Lys Lys Ala 
145                 150                 155                 160 


Leu Ile Ser Arg Glu Ile Lys Ser Ala Val Glu Lys Asp Gly Ala Ser 
                165                 170                 175     


Ala Thr Ile Asp Leu Trp Thr Asp Asn Tyr Ile Lys Arg Asn Phe Leu 
            180                 185                 190         


Gly Val Thr Leu His Tyr His Glu Asn Asn Glu Leu Arg Asp Leu Ile 
        195                 200                 205             


Leu Gly Leu Lys Ser Leu Asp Phe Glu Arg Ser Thr Ala Glu Asn Ile 
    210                 215                 220                 


Tyr Lys Lys Leu Lys Ala Ile Phe Ser Gln Phe Asn Val Glu Asp Leu 
225                 230                 235                 240 


Ser Ser Ile Lys Phe Val Thr Asp Arg Gly Ala Asn Val Val Lys Ser 
                245                 250                 255     


Leu Ala Asn Asn Ile Arg Ile Asn Cys Ser Ser His Leu Leu Ser Asn 
            260                 265                 270         


Val Leu Glu Asn Ser Phe Glu Glu Thr Pro Glu Leu Asn Met Pro Ile 
        275                 280                 285             


Leu Ala Cys Lys Asn Ile Val Lys Tyr Phe Lys Lys Ala Asn Leu Gln 
    290                 295                 300                 


His Arg Leu Arg Ser Ser Leu Lys Ser Glu Cys Pro Thr Arg Trp Asn 
305                 310                 315                 320 


Ser Thr Tyr Thr Met Leu Arg Ser Ile Leu Asp Asn Trp Glu Ser Val 
                325                 330                 335     


Ile Gln Ile Leu Ser Glu Ala Gly Glu Thr Gln Arg Ile Val His Ile 
            340                 345                 350         


Asn Lys Ser Ile Ile Gln Thr Met Val Asn Ile Leu Asp Gly Phe Glu 
        355                 360                 365             


Arg Ile Phe Lys Glu Leu Gln Thr Cys Ser Ser Pro Ser Leu Cys Phe 
    370                 375                 380                 


Val Val Pro Ser Ile Leu Lys Val Lys Glu Ile Cys Ser Pro Asp Val 
385                 390                 395                 400 


Gly Asp Val Ala Asp Ile Ala Lys Leu Lys Val Asn Ile Ile Lys Asn 
                405                 410                 415     


Val Arg Ile Ile Trp Glu Glu Asn Leu Ser Ile Trp His Tyr Thr Ala 
            420                 425                 430         


Phe Phe Phe Tyr Pro Pro Ala Leu His Met Gln Gln Glu Lys Val Ala 
        435                 440                 445             


Gln Ile Lys Glu Phe Cys Leu Ser Lys Met Glu Asp Leu Glu Leu Ile 
    450                 455                 460                 


Asn Arg Met Ser Ser Phe Asn Glu Leu Ser Ala Thr Gln Leu Asn Gln 
465                 470                 475                 480 


Ser Asp Ser Asn Ser His Asn Ser Ile Asp Leu Thr Ser His Ser Lys 
                485                 490                 495     


Pro Val Cys Pro Ser Asp Glu Phe Glu Phe Tyr Arg Lys Glu Ile Val 
            500                 505                 510         


Ile Leu Ser Glu Asp Phe Lys Val Met Glu Trp Trp Asn Leu Asn Ser 
        515                 520                 525             


Lys Lys Tyr Pro Lys Leu Ser Lys Leu Ala Leu Ser Leu Leu Ser Ile 
    530                 535                 540                 


Pro Ala Ser Ser Ala Ala Ser Glu Arg Thr Phe Ser Leu Ala Gly Asn 
545                 550                 555                 560 


Ile Ile Thr Glu Lys Arg Asn Arg Ile Gly Gln Gln Thr Val Asp Ser 
                565                 570                 575     


Leu Leu Phe Leu Asn Ser Phe Tyr Lys Asn Phe Cys Lys Leu Asp Ile 
            580                 585                 590         


<210>  8
<211>  1779
<212>  DNA
<213>  Musca domestica

<400>  8
atggagaaaa tggacaattt ggaagtgaaa gcaaaaatca accaaggatt atataaaatt       60

actccgcgac ataaaggaac aagttttatt tggaacgttt tagcggatat acagaaagaa      120

gacgatacat tggtggaagg gtgggtgttt tgccgaaaat gcgaaaaagt tttaaaatac      180

acaactaggc agacatcaaa cttatgtcgt cataaatgct gtgcctctct aaagcaatcc      240

cgagaattaa aaactgtttc agctgattgc aaaaaggaag caattgaaaa atgtgcacaa      300

tgggtggtac gagattgtcg gcctttttcg gccgtctctg gatccggctt tatcgatatg      360

ataaaatttt ttattaaagt tggagccgaa tatggtgaac atgtcaacgt tgaggaattg      420

ttaccaagtc caataacgct atcgagaaag gtaacttcgg atgcaaaaga aaaaaaagct      480

ctgattagtc gagaaattaa gtctgctgta gagaaagatg gtgcatcagc aacgatagat      540

ttgtggaccg ataattatat aaaacggaat tttttgggag taacgttaca ctaccatgaa      600

aacaatgaac tgcgagatct aattttaggt ttaaagtcct tagattttga aagatccaca      660

gcagaaaata tttataagaa gcttaaagcc attttttcac aattcaacgt cgaagacttg      720

agtagtataa aatttgtgac agatagagga gccaatgtcg taaaatcatt ggcaaataat      780

atcagaatta actgcagcag ccatttgctt tcaaacgtgt tggaaaattc atttgaggag      840

acacctgaac tcaatatgcc tattcttgct tgcaaaaata ttgtaaaata tttcaagaaa      900

gccaatctgc agcacagact tcgaagttct ttaaaaagtg agtgccctac acggtggaat      960

tccacataca cgatgcttcg atctattctc gacaactggg aaagcgtgat tcaaatatta     1020

agtgaggcgg gagagacaca gagaattgtt catataaata agtcgataat tcaaacaatg     1080

gtcaacatcc tcgatgggtt tgaaagaatt tttaaagaat tacaaacatg cagttcacca     1140

tctctgtgtt ttgttgtgcc ttccatttta aaagtaaaag aaatatgttc acctgacgtt     1200

ggcgacgttg cagatatagc aaaattgaaa gtgaacatta taaaaaatgt aagaataata     1260

tgggaagaaa atttaagcat atggcactac acagcatttt ttttctatcc gcccgccttg     1320

catatgcaac aagagaaagt ggcacaaatt aaagaatttt gcttatccaa aatggaagat     1380

ttggaattaa taaaccgcat gagttccttt aacgaattat ccgcaactca gcttaaccag     1440

tcggactcca atagccacaa cagtatagat ttaacatccc attcaaaacc agtgtgtcca     1500

agcgatgaat ttgaatttta tcgtaaagaa atagttattt taagcgaaga ttttaaagtt     1560

atggaatggt ggaatcttaa ttcaaaaaag tatcctaaac tatctaaact ggctttgtcg     1620

ttattatcaa tacctgcaag tagcgctgca tcggaaagga cattttccct agctggaaat     1680

ataataactg aaaagagaaa caggattggg caacaaactg tcgacagctt gttattttta     1740

aattcctttt acaaaaattt ttgtaaatta gatatatag                            1779


<210>  9
<211>  31
<212>  DNA
<213>  Musca domestica


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' T is phosphorylated

<400>  9
tcagagaaca acaacaagtg gcttattttg a                                      31


<210>  10
<211>  30
<212>  DNA
<213>  Musca domestica

<400>  10
tcaaaataag ccacttgttg ttgttctctg                                        30


<210>  11
<211>  55
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic copy of modified Hermes transposon


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' A is biotinylated

<400>  11
ataagtagca agtggcgcat aagtatcaaa ataagccact tgttgttgtt ctctg            55


<210>  12
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  derivatizedhermes transponson


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' C is phosphorylated

<400>  12
ccagagaaca acaacaagtg gcttattttg atacttatgc gccacttgct acttat           56


<210>  13
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  linker for adding tags

<400>  13
tagtccctta agcggagccc tatagtgagt cgtattac                               38


<210>  14
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  linker for ligating tags

<400>  14
gtaatacgac tcactatagg gctccgctta agggac                                 36


<210>  15
<211>  86
<212>  DNA
<213>  artificial sequence

<220>
<223>  proprietary sequence (sequencing primer), 4- bp barcode and the 
       Hermes L-end complementary sequence

<400>  15
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctgc       60

gtcgcataag tatcaaaata agccac                                            86


<210>  16
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  3' linker/adapter specific primer with  3' Illumina tag for use 
       with SEQ ID NO:15

<400>  16
caagcagaag acggcatacg agctcttccg atctgtaata cgactcacta tagggc           56


<210>  17
<211>  92
<212>  DNA
<213>  artificial sequence

<220>
<223>  tag A sequencing priming region, 4 bp barcode and a 30 bp Hermes 
       Transposon end (Biotinylated top strand)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' A is biotinylated

<400>  17
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctgc       60

gttcaaaata agccacttgt tgttgttctc tg                                     92


<210>  18
<211>  93
<212>  DNA
<213>  artificial sequence

<220>
<223>  Phosphorylated bottom strand Hermes LE sequence for use with SEQ 
       ID NO:17


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' C is phosphorylated

<400>  18
ccagagaaca acaacaagtg gcttattttg aacgcagatc ggaagagcgt cgtgtaggga       60

aagagtgtag atctcggtgg tcgccgtatc att                                    93


<210>  19
<211>  92
<212>  DNA
<213>  artificial sequence

<220>
<223>  for introducing tag B

<400>  19
caagcagaag acggcatacg agctcacact ctttccctac acgacgctct tccgatctgc       60

gttcaaaata agccacttgt tgttgttctc tg                                     92


<210>  20
<211>  93
<212>  DNA
<213>  artificial sequence

<220>
<223>  Bottom strand for use with Seq ID No:19

<400>  20
ccagagaaca acaacaagtg gcttattttg aacgcagatc ggaagagcgt cgtgtaggga       60

aagagtgtga gctcgtatgc cgtcttctgc ttg                                    93


