                         SEQUENCE LISTING

<110>  Max-Planck-Gesellschaft zur Frderung der Wissenschaften 
       e.V.
 
<120>  Bioorthogonal chemical inducers of reversible dimerization for 
       control of protein function in cells

<130>  GAR-P03496WO

<160>  6     

<170>  PatentIn version 3.3

<210>  1
<211>  3180
<212>  DNA
<213>  Artificial

<220>
<223>  Cherry-FKBP(F36V)-Atg16L DNA sequence

<400>  1
atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag     60

gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc    120

cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc    180

ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac    240

cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc    300

gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac    360

ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta    420

atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc    480

gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct    540

gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc    600

aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa    660

cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga    720

tctcgagcta tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc    780

aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt    840

gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc    900

cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata    960

tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact   1020

ctcgtcttcg atgtggagct tctaaaactg gaacaagctt cgaattctgc agtcgacggt   1080

accatgggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc   1140

ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc   1200

tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc   1260

tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca   1320

gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc   1380

ttcgatgtgg agcttctaaa actggaagga tccatgtcgt cgggcctgcg cgccgcagac   1440

tttccccgct ggaagcgtca catcgcggag gaactgaggc gccgggaccg actgcagagg   1500

caggcgttcg aggagatcat tctgcagtat accaagttgc tggaaaagtc agatcttcat   1560

tcagtattga cccagaaact acaagcagaa aagcatgaca tgccaaatag gcatgaaata   1620

agtcctggac atgatggtgc gtggaatgat agtcaactac aagaaatggc ccagttgagg   1680

atcaaacacc aggaagagct gaccgaactg cacaagaagc gtggggagtt agctcagttg   1740

gtgattgacc tgaacaacca aatgcagcag aaggacaagg agatacagat gaatgaagca   1800

aagatttcgg agtatttaca gaccatctct gacctggaga caaactgcct ggacctgcgc   1860

accaaactgc aggacctcga ggtagccaac cagaccctga aggatgagta tgacgccctg   1920

cagattactt ttactgccct agaagagaaa ctgaggaaaa ctactgagga gaaccaggaa   1980

ctggtcacca gatggatggc tgagaaggcc caagaagcca atcgcctcaa tgcagagaat   2040

gagaaggact ccaggaggcg tcaagcacgg ctgcagaagg agcttgcaga agcagcaaag   2100

gaacctctac ctgttgaaca ggatgatgac attgaagtca ttgtggatga gacctcagac   2160

cacacagaag agacctctcc cgtccgagct gtcagcagag cagctacgag acgctctgtc   2220

tcttccatcc cagtccccca ggatatcatg gacactcatc ctgcttctgg taaagatgtg   2280

agagtcccaa ctactgcctc gtatgtcttc gatgcgcatg acggagaggt caacgcagtg   2340

cagttcagtc caggctcccg gttgctggcc actggaggca tggaccgcag ggtgaaactt   2400

tgggaagcat tcggagataa atgtgaattc aagggctccc tgtctggcag taatgctgga   2460

attacaagca ttgaatttga tagtgctgga gcttacctat tagcagcttc aaatgatttt   2520

gcaagccgaa tctggactgt ggatgattat cgattacggc acacactcac aggccacagc   2580

gggaaagtcc tctctgccaa gttcctgctg gacaatgcac ggattgtctc aggaagtcac   2640

gaccggaccc tcaaactctg ggatctccgc agcaaagtct gcataaaaac agtgtttgca   2700

ggatccagct gcaatgacat tgtttgcact gaacaatgtg taatgagtgg acattttgac   2760

aagaaaattc gtttctggga tatccggtca gagagtgtgg tccgagagat ggaactgtta   2820

gggaagatca ctgctctgga cctaaaccct gagagaactg agctcctgag ctgctcccgt   2880

gatgacctgc taaaagtcat cgacctccgg acaaatgcag tcaaacagac attcagtgcg   2940

cctggattca aatgcggctc tgactggacc cgggttgtct tcagccctga tggcagttac   3000

gtggcagcag gctcagccga gggttctctt tatgtctgga gtgtgctgac agggaaagtg   3060

gagaaggttc tttcaaaaca gcacagctct tctatcaatg cggtggcgtg ggccccctcg   3120

ggcttacatg ttgtcagtgt ggacaaagga agcagagcta tgctgtgggc acagccttga   3180


<210>  2
<211>  1059
<212>  PRT
<213>  Artificial

<220>
<223>  Cherry-FKBP(F36V)-Atg16 protein sequence

<400>  2

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Ser Gly Leu Arg 
225                 230                 235                 240 


Ser Arg Ala Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
                245                 250                 255     


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
            260                 265                 270         


Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys 
        275                 280                 285             


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
    290                 295                 300                 


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
305                 310                 315                 320 


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
                325                 330                 335     


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gln 
            340                 345                 350         


Ala Ser Asn Ser Ala Val Asp Gly Thr Met Gly Val Gln Val Glu Thr 
        355                 360                 365             


Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys 
    370                 375                 380                 


Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser 
385                 390                 395                 400 


Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu 
                405                 410                 415     


Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln 
            420                 425                 430         


Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly 
        435                 440                 445             


His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu 
    450                 455                 460                 


Leu Leu Lys Leu Glu Gly Ser Met Ser Ser Gly Leu Arg Ala Ala Asp 
465                 470                 475                 480 


Phe Pro Arg Trp Lys Arg His Ile Ala Glu Glu Leu Arg Arg Arg Asp 
                485                 490                 495     


Arg Leu Gln Arg Gln Ala Phe Glu Glu Ile Ile Leu Gln Tyr Thr Lys 
            500                 505                 510         


Leu Leu Glu Lys Ser Asp Leu His Ser Val Leu Thr Gln Lys Leu Gln 
        515                 520                 525             


Ala Glu Lys His Asp Met Pro Asn Arg His Glu Ile Ser Pro Gly His 
    530                 535                 540                 


Asp Gly Ala Trp Asn Asp Ser Gln Leu Gln Glu Met Ala Gln Leu Arg 
545                 550                 555                 560 


Ile Lys His Gln Glu Glu Leu Thr Glu Leu His Lys Lys Arg Gly Glu 
                565                 570                 575     


Leu Ala Gln Leu Val Ile Asp Leu Asn Asn Gln Met Gln Gln Lys Asp 
            580                 585                 590         


Lys Glu Ile Gln Met Asn Glu Ala Lys Ile Ser Glu Tyr Leu Gln Thr 
        595                 600                 605             


Ile Ser Asp Leu Glu Thr Asn Cys Leu Asp Leu Arg Thr Lys Leu Gln 
    610                 615                 620                 


Asp Leu Glu Val Ala Asn Gln Thr Leu Lys Asp Glu Tyr Asp Ala Leu 
625                 630                 635                 640 


Gln Ile Thr Phe Thr Ala Leu Glu Glu Lys Leu Arg Lys Thr Thr Glu 
                645                 650                 655     


Glu Asn Gln Glu Leu Val Thr Arg Trp Met Ala Glu Lys Ala Gln Glu 
            660                 665                 670         


Ala Asn Arg Leu Asn Ala Glu Asn Glu Lys Asp Ser Arg Arg Arg Gln 
        675                 680                 685             


Ala Arg Leu Gln Lys Glu Leu Ala Glu Ala Ala Lys Glu Pro Leu Pro 
    690                 695                 700                 


Val Glu Gln Asp Asp Asp Ile Glu Val Ile Val Asp Glu Thr Ser Asp 
705                 710                 715                 720 


His Thr Glu Glu Thr Ser Pro Val Arg Ala Val Ser Arg Ala Ala Thr 
                725                 730                 735     


Arg Arg Ser Val Ser Ser Ile Pro Val Pro Gln Asp Ile Met Asp Thr 
            740                 745                 750         


His Pro Ala Ser Gly Lys Asp Val Arg Val Pro Thr Thr Ala Ser Tyr 
        755                 760                 765             


Val Phe Asp Ala His Asp Gly Glu Val Asn Ala Val Gln Phe Ser Pro 
    770                 775                 780                 


Gly Ser Arg Leu Leu Ala Thr Gly Gly Met Asp Arg Arg Val Lys Leu 
785                 790                 795                 800 


Trp Glu Ala Phe Gly Asp Lys Cys Glu Phe Lys Gly Ser Leu Ser Gly 
                805                 810                 815     


Ser Asn Ala Gly Ile Thr Ser Ile Glu Phe Asp Ser Ala Gly Ala Tyr 
            820                 825                 830         


Leu Leu Ala Ala Ser Asn Asp Phe Ala Ser Arg Ile Trp Thr Val Asp 
        835                 840                 845             


Asp Tyr Arg Leu Arg His Thr Leu Thr Gly His Ser Gly Lys Val Leu 
    850                 855                 860                 


Ser Ala Lys Phe Leu Leu Asp Asn Ala Arg Ile Val Ser Gly Ser His 
865                 870                 875                 880 


Asp Arg Thr Leu Lys Leu Trp Asp Leu Arg Ser Lys Val Cys Ile Lys 
                885                 890                 895     


Thr Val Phe Ala Gly Ser Ser Cys Asn Asp Ile Val Cys Thr Glu Gln 
            900                 905                 910         


Cys Val Met Ser Gly His Phe Asp Lys Lys Ile Arg Phe Trp Asp Ile 
        915                 920                 925             


Arg Ser Glu Ser Val Val Arg Glu Met Glu Leu Leu Gly Lys Ile Thr 
    930                 935                 940                 


Ala Leu Asp Leu Asn Pro Glu Arg Thr Glu Leu Leu Ser Cys Ser Arg 
945                 950                 955                 960 


Asp Asp Leu Leu Lys Val Ile Asp Leu Arg Thr Asn Ala Val Lys Gln 
                965                 970                 975     


Thr Phe Ser Ala Pro Gly Phe Lys Cys Gly Ser Asp Trp Thr Arg Val 
            980                 985                 990         


Val Phe Ser Pro Asp Gly Ser Tyr  Val Ala Ala Gly Ser  Ala Glu Gly 
        995                 1000                 1005             


Ser Leu  Tyr Val Trp Ser Val  Leu Thr Gly Lys Val  Glu Lys Val 
    1010                 1015                 1020             


Leu Ser  Lys Gln His Ser Ser  Ser Ile Asn Ala Val  Ala Trp Ala 
    1025                 1030                 1035             


Pro Ser  Gly Leu His Val Val  Ser Val Asp Lys Gly  Ser Arg Ala 
    1040                 1045                 1050             


Met Leu  Trp Ala Gln Pro 
    1055                 


<210>  3
<211>  2778
<212>  DNA
<213>  Artificial

<220>
<223>  Cherry-FKBP(F36V)-Beclin1 DNA sequence

<400>  3
atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag     60

gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc    120

cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc    180

ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac    240

cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc    300

gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac    360

ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta    420

atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc    480

gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct    540

gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc    600

aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa    660

cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga    720

tctcgagcta tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc    780

aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt    840

gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc    900

cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata    960

tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact   1020

ctcgtcttcg atgtggagct tctaaaactg gaacaagctt cgaattctgc agtcgacggt   1080

accatgggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc   1140

ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc   1200

tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc   1260

tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca   1320

gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc   1380

ttcgatgtgg agcttctaaa actggaagga tccaccggat ctagaatgga agggtctaag   1440

acgtccaaca acagcaccat gcaggtgagc ttcgtgtgcc agcgctgcag ccagcccctg   1500

aaactggaca cgagtttcaa gatcctggac cgtgtcacca tccaggaact cacagctcca   1560

ttacttacca cagcccaggc gaaaccagga gagacccagg aggaagagac taactcagga   1620

gaggagccat ttattgaaac tcctcgccag gatggtgtct ctcgcagatt catcccccca   1680

gccaggatga tgtccacaga aagtgccaac agcttcactc tgattgggga ggcatctgat   1740

ggcggcacca tggagaacct cagccgaaga ctgaaggtca ctggggacct ttttgacatc   1800

atgtcgggcc agacagatgt ggatcaccca ctctgtgagg aatgcacaga tactctttta   1860

gaccagctgg acactcagct caacgtcact gaaaatgagt gtcagaacta caaacgctgt   1920

ttggagatct tagagcaaat gaatgaggat gacagtgaac agttacagat ggagctaaag   1980

gagctggcac tagaggagga gaggctgatc caggagctgg aagacgtgga aaagaaccgc   2040

aagatagtgg cagaaaatct cgagaaggtc caggctgagg ctgagagact ggatcaggag   2100

gaagctcagt atcagagaga atacagtgaa tttaaacgac agcagctgga gctggatgat   2160

gagctgaaga gtgttgaaaa ccagatgcgt tatgcccaga cgcagctgga taagctgaag   2220

aaaaccaacg tctttaatgc aaccttccac atctggcaca gtggacagtt tggcacaatc   2280

aataacttca ggctgggtcg cctgcccagt gttcccgtgg aatggaatga gattaatgct   2340

gcttggggcc agactgtgtt gctgctccat gctctggcca ataagatggg tctgaaattt   2400

cagagatacc gacttgttcc ttacggaaac cattcatatc tggagtctct gacagacaaa   2460

tctaaggagc tgccgttata ctgttctggg gggttgcggt ttttctggga caacaagttt   2520

gaccatgcaa tggtggcttt cctggactgt gtgcagcagt tcaaagaaga ggttgagaaa   2580

ggcgagacac gtttttgtct tccctacagg atggatgtgg agaaaggcaa gattgaagac   2640

acaggaggca gtggcggctc ctattccatc aaaacccagt ttaactctga ggagcagtgg   2700

acaaaagctc tcaagttcat gctgacgaat cttaagtggg gtcttgcttg ggtgtcctca   2760

caattttata acaaataa                                                 2778


<210>  4
<211>  925
<212>  PRT
<213>  Artificial

<220>
<223>  Cherry-FKBP(F36V)-Beclin1 protein sequence

<400>  4

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Ser Gly Leu Arg 
225                 230                 235                 240 


Ser Arg Ala Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
                245                 250                 255     


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
            260                 265                 270         


Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys 
        275                 280                 285             


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
    290                 295                 300                 


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
305                 310                 315                 320 


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
                325                 330                 335     


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gln 
            340                 345                 350         


Ala Ser Asn Ser Ala Val Asp Gly Thr Met Gly Val Gln Val Glu Thr 
        355                 360                 365             


Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys 
    370                 375                 380                 


Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser 
385                 390                 395                 400 


Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu 
                405                 410                 415     


Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln 
            420                 425                 430         


Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly 
        435                 440                 445             


His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu 
    450                 455                 460                 


Leu Leu Lys Leu Glu Gly Ser Thr Gly Ser Arg Met Glu Gly Ser Lys 
465                 470                 475                 480 


Thr Ser Asn Asn Ser Thr Met Gln Val Ser Phe Val Cys Gln Arg Cys 
                485                 490                 495     


Ser Gln Pro Leu Lys Leu Asp Thr Ser Phe Lys Ile Leu Asp Arg Val 
            500                 505                 510         


Thr Ile Gln Glu Leu Thr Ala Pro Leu Leu Thr Thr Ala Gln Ala Lys 
        515                 520                 525             


Pro Gly Glu Thr Gln Glu Glu Glu Thr Asn Ser Gly Glu Glu Pro Phe 
    530                 535                 540                 


Ile Glu Thr Pro Arg Gln Asp Gly Val Ser Arg Arg Phe Ile Pro Pro 
545                 550                 555                 560 


Ala Arg Met Met Ser Thr Glu Ser Ala Asn Ser Phe Thr Leu Ile Gly 
                565                 570                 575     


Glu Ala Ser Asp Gly Gly Thr Met Glu Asn Leu Ser Arg Arg Leu Lys 
            580                 585                 590         


Val Thr Gly Asp Leu Phe Asp Ile Met Ser Gly Gln Thr Asp Val Asp 
        595                 600                 605             


His Pro Leu Cys Glu Glu Cys Thr Asp Thr Leu Leu Asp Gln Leu Asp 
    610                 615                 620                 


Thr Gln Leu Asn Val Thr Glu Asn Glu Cys Gln Asn Tyr Lys Arg Cys 
625                 630                 635                 640 


Leu Glu Ile Leu Glu Gln Met Asn Glu Asp Asp Ser Glu Gln Leu Gln 
                645                 650                 655     


Met Glu Leu Lys Glu Leu Ala Leu Glu Glu Glu Arg Leu Ile Gln Glu 
            660                 665                 670         


Leu Glu Asp Val Glu Lys Asn Arg Lys Ile Val Ala Glu Asn Leu Glu 
        675                 680                 685             


Lys Val Gln Ala Glu Ala Glu Arg Leu Asp Gln Glu Glu Ala Gln Tyr 
    690                 695                 700                 


Gln Arg Glu Tyr Ser Glu Phe Lys Arg Gln Gln Leu Glu Leu Asp Asp 
705                 710                 715                 720 


Glu Leu Lys Ser Val Glu Asn Gln Met Arg Tyr Ala Gln Thr Gln Leu 
                725                 730                 735     


Asp Lys Leu Lys Lys Thr Asn Val Phe Asn Ala Thr Phe His Ile Trp 
            740                 745                 750         


His Ser Gly Gln Phe Gly Thr Ile Asn Asn Phe Arg Leu Gly Arg Leu 
        755                 760                 765             


Pro Ser Val Pro Val Glu Trp Asn Glu Ile Asn Ala Ala Trp Gly Gln 
    770                 775                 780                 


Thr Val Leu Leu Leu His Ala Leu Ala Asn Lys Met Gly Leu Lys Phe 
785                 790                 795                 800 


Gln Arg Tyr Arg Leu Val Pro Tyr Gly Asn His Ser Tyr Leu Glu Ser 
                805                 810                 815     


Leu Thr Asp Lys Ser Lys Glu Leu Pro Leu Tyr Cys Ser Gly Gly Leu 
            820                 825                 830         


Arg Phe Phe Trp Asp Asn Lys Phe Asp His Ala Met Val Ala Phe Leu 
        835                 840                 845             


Asp Cys Val Gln Gln Phe Lys Glu Glu Val Glu Lys Gly Glu Thr Arg 
    850                 855                 860                 


Phe Cys Leu Pro Tyr Arg Met Asp Val Glu Lys Gly Lys Ile Glu Asp 
865                 870                 875                 880 


Thr Gly Gly Ser Gly Gly Ser Tyr Ser Ile Lys Thr Gln Phe Asn Ser 
                885                 890                 895     


Glu Glu Gln Trp Thr Lys Ala Leu Lys Phe Met Leu Thr Asn Leu Lys 
            900                 905                 910         


Trp Gly Leu Ala Trp Val Ser Ser Gln Phe Tyr Asn Lys 
        915                 920                 925 


<210>  5
<211>  1785
<212>  DNA
<213>  Artificial

<220>
<223>  BFP-eDHFR-PM DNA sequence

<400>  5
atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac     60

aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc    120

atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact    180

agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc    240

aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc    300

gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag    360

atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg    420

gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg    480

gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc    540

aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa    600

agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga    660

tactgcgacc tccctagcaa actggggcac aagcttaatt ccggactcag atctcgagct    720

atgatcagtc tgattgcggc gttagcggta gatcgcgtta tcggcatgga aaacgccatg    780

ccgtggaacc tgcctgccga tctcgcctgg tttaaacgca acaccttaaa taaacccgtg    840

attatgggcc gccatacctg ggaatcaatc ggtcgtccgt tgccaggacg caaaaatatt    900

atcctcagca gtcaaccggg tacggacgat cgcgtaacgt gggtgaagtc ggtggatgaa    960

gccatcgcgg cgtgtggtga cgtaccagaa atcatggtga ttggcggcgg tcgcgtttat   1020

gaacagttct tgccaaaagc gcaaaaactg tatctgacgc atatcgacgc agaagtggaa   1080

ggcgacaccc atttcccgga ttacgagccg gatgactggg aatcggtatt cagcgaattc   1140

cacgatgctg atgcgcagaa ctctcacagc tattgctttg agattctgga gcggcggcaa   1200

gcttcgaatt ctgcagtcga cggtaccatg atcagtctga ttgcggcgtt agcggtagat   1260

cgcgttatcg gcatggaaaa cgccatgccg tggaacctgc ctgccgatct cgcctggttt   1320

aaacgcaaca ccttaaataa acccgtgatt atgggccgcc atacctggga atcaatcggt   1380

cgtccgttgc caggacgcaa aaatattatc ctcagcagtc aaccgggtac ggacgatcgc   1440

gtaacgtggg tgaagtcggt ggatgaagcc atcgcggcgt gtggtgacgt accagaaatc   1500

atggtgattg gcggcggtcg cgtttatgaa cagttcttgc caaaagcgca aaaactgtat   1560

ctgacgcata tcgacgcaga agtggaaggc gacacccatt tcccggatta cgagccggat   1620

gactgggaat cggtattcag cgaattccac gatgctgatg cgcagaactc tcacagctat   1680

tgctttgaga ttctggagcg gcggggatcc ggcggttccg gtaagatgag caaagatggt   1740

aaaaagaaga aaaagaagtc aaagacaaag tgtgtaatta tgtaa                   1785


<210>  6
<211>  594
<212>  PRT
<213>  Artificial

<220>
<223>  BFP-eDHFR-PM protein sequence

<400>  6

Met Ser Glu Leu Ile Lys Glu Asn Met His Met Lys Leu Tyr Met Glu 
1               5                   10                  15      


Gly Thr Val Asp Asn His His Phe Lys Cys Thr Ser Glu Gly Glu Gly 
            20                  25                  30          


Lys Pro Tyr Glu Gly Thr Gln Thr Met Arg Ile Lys Val Val Glu Gly 
        35                  40                  45              


Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr Ser Phe Leu Tyr 
    50                  55                  60                  


Gly Ser Lys Thr Phe Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe 
65                  70                  75                  80  


Lys Gln Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg Val Thr Thr Tyr 
                85                  90                  95      


Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asp 
            100                 105                 110         


Gly Cys Leu Ile Tyr Asn Val Lys Ile Arg Gly Val Asn Phe Thr Ser 
        115                 120                 125             


Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly Trp Glu Ala Phe Thr 
    130                 135                 140                 


Glu Thr Leu Tyr Pro Ala Asp Gly Gly Leu Glu Gly Arg Asn Asp Met 
145                 150                 155                 160 


Ala Leu Lys Leu Val Gly Gly Ser His Leu Ile Ala Asn Ile Lys Thr 
                165                 170                 175     


Thr Tyr Arg Ser Lys Lys Pro Ala Lys Asn Leu Lys Met Pro Gly Val 
            180                 185                 190         


Tyr Tyr Val Asp Tyr Arg Leu Glu Arg Ile Lys Glu Ala Asn Asn Glu 
        195                 200                 205             


Thr Tyr Val Glu Gln His Glu Val Ala Val Ala Arg Tyr Cys Asp Leu 
    210                 215                 220                 


Pro Ser Lys Leu Gly His Lys Leu Asn Ser Gly Leu Arg Ser Arg Ala 
225                 230                 235                 240 


Met Ile Ser Leu Ile Ala Ala Leu Ala Val Asp Arg Val Ile Gly Met 
                245                 250                 255     


Glu Asn Ala Met Pro Trp Asn Leu Pro Ala Asp Leu Ala Trp Phe Lys 
            260                 265                 270         


Arg Asn Thr Leu Asn Lys Pro Val Ile Met Gly Arg His Thr Trp Glu 
        275                 280                 285             


Ser Ile Gly Arg Pro Leu Pro Gly Arg Lys Asn Ile Ile Leu Ser Ser 
    290                 295                 300                 


Gln Pro Gly Thr Asp Asp Arg Val Thr Trp Val Lys Ser Val Asp Glu 
305                 310                 315                 320 


Ala Ile Ala Ala Cys Gly Asp Val Pro Glu Ile Met Val Ile Gly Gly 
                325                 330                 335     


Gly Arg Val Tyr Glu Gln Phe Leu Pro Lys Ala Gln Lys Leu Tyr Leu 
            340                 345                 350         


Thr His Ile Asp Ala Glu Val Glu Gly Asp Thr His Phe Pro Asp Tyr 
        355                 360                 365             


Glu Pro Asp Asp Trp Glu Ser Val Phe Ser Glu Phe His Asp Ala Asp 
    370                 375                 380                 


Ala Gln Asn Ser His Ser Tyr Cys Phe Glu Ile Leu Glu Arg Arg Gln 
385                 390                 395                 400 


Ala Ser Asn Ser Ala Val Asp Gly Thr Met Ile Ser Leu Ile Ala Ala 
                405                 410                 415     


Leu Ala Val Asp Arg Val Ile Gly Met Glu Asn Ala Met Pro Trp Asn 
            420                 425                 430         


Leu Pro Ala Asp Leu Ala Trp Phe Lys Arg Asn Thr Leu Asn Lys Pro 
        435                 440                 445             


Val Ile Met Gly Arg His Thr Trp Glu Ser Ile Gly Arg Pro Leu Pro 
    450                 455                 460                 


Gly Arg Lys Asn Ile Ile Leu Ser Ser Gln Pro Gly Thr Asp Asp Arg 
465                 470                 475                 480 


Val Thr Trp Val Lys Ser Val Asp Glu Ala Ile Ala Ala Cys Gly Asp 
                485                 490                 495     


Val Pro Glu Ile Met Val Ile Gly Gly Gly Arg Val Tyr Glu Gln Phe 
            500                 505                 510         


Leu Pro Lys Ala Gln Lys Leu Tyr Leu Thr His Ile Asp Ala Glu Val 
        515                 520                 525             


Glu Gly Asp Thr His Phe Pro Asp Tyr Glu Pro Asp Asp Trp Glu Ser 
    530                 535                 540                 


Val Phe Ser Glu Phe His Asp Ala Asp Ala Gln Asn Ser His Ser Tyr 
545                 550                 555                 560 


Cys Phe Glu Ile Leu Glu Arg Arg Gly Ser Gly Gly Ser Gly Lys Met 
                565                 570                 575     


Ser Lys Asp Gly Lys Lys Lys Lys Lys Lys Ser Lys Thr Lys Cys Val 
            580                 585                 590         


Ile Met 
        


