                         SEQUENCE LISTING

<110>  Jacobs, Steven
 
<120>  Non-natural consensus albumin binding domains

<130>  JBI5009WOPCT

<140>  To be determined
<141>  2013-03-23

<150>  61/651,642
<151>  2012-05-25

<150>  61/776,918
<151>  2013-03-12

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  44
<212>  PRT
<213>  Streptococcus sp. G148

<400>  1

Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 
1               5                   10                  15      


Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asn Asn Ala Lys Thr Val Glu 
            20                  25                  30          


Gly Val Lys Ala Leu Ile Asp Glu Ile Leu Ala Ala 
        35                  40                  


<210>  2
<211>  44
<212>  PRT
<213>  Streptococcus canis

<400>  2

Leu Ser Glu Ala Lys Glu Met Ala Ile Arg Glu Leu Asp Ala Gln Gly 
1               5                   10                  15      


Val Ser Asp Phe Tyr Lys Asn Lys Ile Asn Asn Ala Lys Thr Val Glu 
            20                  25                  30          


Gly Val Val Ala Leu Lys Asp Leu Ile Leu Asn Ser 
        35                  40                  


<210>  3
<211>  42
<212>  PRT
<213>  Streptococcus canis

<400>  3

Leu Asp Gln Ala Lys Gln Ala Ala Leu Lys Glu Phe Asp Arg Tyr Gly 
1               5                   10                  15      


Val Ser Asn Tyr Tyr Lys Asn Leu Ile Asn Lys Ala Lys Thr Val Glu 
            20                  25                  30          


Gly Ile Met Glu Leu Gln Ala Gln Val Val 
        35                  40          


<210>  4
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain

<400>  4

Leu Ala Gln Ala Lys Glu Ala Ala Ile Lys Glu Leu Lys Gln Tyr Gly 
1               5                   10                  15      


Ile Gly Asp Tyr Tyr Ile Lys Leu Ile Asn Asn Ala Lys Thr Val Glu 
            20                  25                  30          


Gly Val Glu Ser Leu Lys Asn Glu Ile Leu Lys Ala 
        35                  40                  


<210>  5
<211>  44
<212>  PRT
<213>  Streptococcus equi

<400>  5


Leu Leu Lys Ala Lys Glu Ala Ala Ile Asn Glu Leu Lys Gln Tyr Gly 
1               5                   10                  15      


Ile Ser Asp Tyr Tyr Val Thr Leu Ile Asn Lys Ala Lys Thr Val Glu 
            20                  25                  30          


Gly Val Asn Ala Leu Lys Ala Glu Ile Leu Ser Ala 
        35                  40                  


<210>  6
<211>  45
<212>  PRT
<213>  Finecoldia Magna

<400>  6

Leu Lys Asn Ala Lys Glu Asp Ala Ile Lys Glu Leu Lys Glu Ala Gly 
1               5                   10                  15      


Ile Lys Ser Gln Phe Phe Phe Asn Leu Ile Asn Asn Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Glu Ser Leu Lys Asn Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  7
<211>  45
<212>  PRT
<213>  Finecoldia Magna

<400>  7

Leu Lys Asp Ala Lys Glu Lys Ala Ile Glu Ala Ile Arg Lys Glu Gly 
1               5                   10                  15      


Val Lys Ser Lys Leu Tyr Glu Asp Leu Ile Asn Lys Ala Lys Thr Ile 
            20                  25                  30          


Asp Gly Val Asn Ala Leu Arg Asp Gln Ile Ile Glu Ala 
        35                  40                  45  


<210>  8
<211>  45
<212>  PRT
<213>  Peptostreptococcus magnus

<400>  8

Leu Lys Asn Ala Lys Glu Glu Ala Ile Lys Glu Leu Lys Glu Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Leu Tyr Phe Ser Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Glu Ala Leu Lys Asn Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  9
<211>  45
<212>  PRT
<213>  Peptostreptococcus magnus

<400>  9

Leu Lys Asn Ala Lys Glu Ala Ala Ile Lys Glu Leu Lys Glu Ala Gly 
1               5                   10                  15      


Ile Thr Ala Glu Tyr Leu Phe Asn Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Glu Ser Leu Lys Asn Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  10
<211>  45
<212>  PRT
<213>  Peptostreptococcus magnus

<400>  10

Leu Lys Asn Ala Lys Glu Asp Ala Ile Lys Glu Leu Lys Glu Ala Gly 
1               5                   10                  15      


Ile Ser Ser Asp Ile Tyr Phe Asp Ala Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Glu Ala Leu Lys Asn Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  11
<211>  45
<212>  PRT
<213>  Peptostreptococcus magnus

<400>  11

Leu Lys Asn Ala Lys Glu Asp Ala Ile Lys Glu Leu Lys Glu Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Ile Tyr Phe Asp Ala Ile Asn Lys Ala Lys Thr Ile 
            20                  25                  30          


Glu Gly Val Glu Ala Leu Lys Asn Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  12
<211>  45
<212>  PRT
<213>  Finegoldia magna

<400>  12

Leu Ala Glu Ala Lys Lys Val Ala His Glu Glu Phe Thr Lys Ala Gly 
1               5                   10                  15      


Ile Thr Gly Lys Ile Phe His Asp Ala Ile Asp Ala Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Leu Lys Ala Tyr Val Ala Glu Thr Leu Ala Ala 
        35                  40                  45  


<210>  13
<211>  45
<212>  PRT
<213>  Finegoldia magna

<400>  13

Leu Lys Glu Ala Lys Glu Lys Ala Val Glu Glu Leu Lys Glu Asn Gly 
1               5                   10                  15      


Ile Thr Ser Glu Lys Tyr Ile Asp Gln Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Ile Lys Ala 
        35                  40                  45  


<210>  14
<211>  45
<212>  PRT
<213>  Finegoldia magnoa

<400>  14

Leu Lys Glu Ala Lys Glu Lys Ala Val Glu Glu Leu Lys Asn Asn Gly 
1               5                   10                  15      


Ile Thr Ser Glu Lys Tyr Ile Asp Gln Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Ile Lys Ala 
        35                  40                  45  


<210>  15
<211>  45
<212>  PRT
<213>  Lactobacillus iners

<400>  15

Leu Ala Glu Ala Lys Lys Val Ala His Glu Glu Phe Thr Lys Ala Gly 
1               5                   10                  15      


Ile Thr Gly Lys Ile Phe His Asp Ala Ile Asp Ala Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Leu Gln Ala Tyr Val Ala Glu Thr Leu Ala Ala 
        35                  40                  45  


<210>  16
<211>  45
<212>  PRT
<213>  Peptostreptococcus magnus

<400>  16

Leu Lys Asn Ala Lys Glu Asp Ala Ile Ala Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Phe Tyr Phe Asn Ala Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Glu Val Asn Ala Leu Lys Asn Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  17
<211>  45
<212>  PRT
<213>  Finegoldia magna

<400>  17

Leu Lys Glu Ala Lys Glu Lys Ala Val Glu Glu Leu Lys Asn Asn Gly 
1               5                   10                  15      


Ile Thr Ser Glu Lys Tyr Ile Glu Gln Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Ile Lys Ala 
        35                  40                  45  


<210>  18
<211>  45
<212>  PRT
<213>  Lactobacillus iners

<400>  18

Leu Ala Glu Ala Lys Asn Val Ala His Ala Glu Phe Thr Lys Ala Gly 
1               5                   10                  15      


Ile Thr Gly Lys Ile Phe His Asp Ala Ile Asp Ala Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Leu Gln Ala Tyr Val Ala Glu Thr Leu Ala Ala 
        35                  40                  45  


<210>  19
<211>  45
<212>  PRT
<213>  Lactobacillus iners

<400>  19

Leu Ala Glu Ala Lys Lys Ala Ala His Glu Glu Phe Thr Lys Ala Gly 
1               5                   10                  15      


Ile Thr Gly Lys Ile Phe His Asp Ala Ile Asp Ala Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Leu Gln Ala Tyr Val Ala Glu Thr Leu Lys Ala 
        35                  40                  45  


<210>  20
<211>  45
<212>  PRT
<213>  Finegoldia magna

<400>  20

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Asn Asn Gly 
1               5                   10                  15      


Ile Thr Ser Glu Lys Tyr Ile Glu Gln Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Ile Lys Ser 
        35                  40                  45  


<210>  21
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain

<400>  21

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  22
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa1 may be any amino acid

<220>
<221>  MOD_RES
<222>  (22)..(22)
<223>  Xaa2 may be any amino acid

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  Xaa3 may be any amino acid

<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Xaa4 may be any amino acid

<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  Xaa5 may be any amino acid

<220>
<221>  MOD_RES
<222>  (41)..(41)
<223>  Xaa6 may be any amino acid

<400>  22

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Xaa Xaa Phe Asp Leu Ile Asn Lys Ala Xaa Xaa Val 
            20                  25                  30          


Glu Gly Val Asn Xaa Leu Lys Asp Xaa Ile Leu Lys Ala 
        35                  40                  45  


<210>  23
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa1 is Lys, Arg, Asp, Glu, Ala, Gly, Phe or Tyr

<220>
<221>  MOD_RES
<222>  (22)..(22)
<223>  Xaa2 is Ala, Ser, Val, Lys, Arg, Asp, Glu or Tyr

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  Xaa3 is Asp, Glu or Lys

<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Xaa4 is Ala, Gly or Thr

<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  Xaa5 is  Val, Tyr or Ala

<220>
<221>  MOD_RES
<222>  (41)..(41)
<223>  Xaa6 is Qln, Asn, Lys, Arg or Glu

<400>  23

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Xaa Xaa Phe Asp Leu Ile Asn Lys Ala Xaa Xaa Val 
            20                  25                  30          


Glu Gly Val Asn Xaa Leu Lys Asp Xaa Ile Leu Lys Ala 
        35                  40                  45  


<210>  24
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa1 is Lys, Ala or Tyr

<220>
<221>  MOD_RES
<222>  (22)..(22)
<223>  Xaa2 is Ala, Ser, Val or Tyr

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  Xaa3 is Asp or Lys

<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Xaa4 is Ala or Thr

<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  Xaa5 is Val, Tyr or Ala

<220>
<221>  MOD_RES
<222>  (41)..(41)
<223>  Xaa6 is Qln or Glu

<400>  24

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Xaa Xaa Phe Asp Leu Ile Asn Lys Ala Xaa Xaa Val 
            20                  25                  30          


Glu Gly Val Asn Xaa Leu Lys Asp Xaa Ile Leu Lys Ala 
        35                  40                  45  


<210>  25
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon2

<400>  25

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Ala Tyr Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  26
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon3

<400>  26

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Lys Tyr Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  27
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon4

<400>  27

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Ala Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  28
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon5

<400>  28

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Ser Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  29
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon6

<400>  29

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Val Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  30
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon7

<400>  30

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Gln Ile Leu Lys Ala 
        35                  40                  45  


<210>  31
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon8

<400>  31

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys Ala Asp Thr Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  32
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon9

<400>  32

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys Ala Lys Ala Val 
            20                  25                  30          


Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  33
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon10

<400>  33

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Val Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  34
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-natural albumin binding domain ABDCon11

<400>  34

Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly 
1               5                   10                  15      


Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys Ala Lys Thr Val 
            20                  25                  30          


Glu Gly Val Asn Tyr Leu Lys Asp Glu Ile Leu Lys Ala 
        35                  40                  45  


<210>  35
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized cDNA for expressing non-natural albumin bindind 
       domain ABDCon

<400>  35
ctgaaagaag cgaaagaaaa agcgattgaa gaactgaaaa aagcgggcat taccagcgat       60

tattattttg atctgattaa caaagcgaaa accgtggaag gcgtgaacgc gctgaaagat      120

gaaattctga aagcg                                                       135


<210>  36
<211>  585
<212>  PRT
<213>  Homo sapiens

<400>  36

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     



Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 


<210>  37
<211>  584
<212>  PRT
<213>  Macaca mulatta

<400>  37

Asp Thr His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu His Phe Lys Gly Leu Val Leu Val Ala Phe Ser Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Glu His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Pro Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Ala Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Val Ala Arg 
    130                 135                 140                 



Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Ala Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Ala Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Asp 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Lys Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Met Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Asp Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Leu Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Tyr Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Met Leu Leu Leu Arg Leu Ala Lys Ala 
            340                 345                 350         


Tyr Glu Ala Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Gln Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Val Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ala Lys Cys Cys Lys Leu Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Arg Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Glu Lys Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Leu Asp Glu Ala 
                485                 490                 495     


Tyr Val Pro Lys Ala Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Met Cys Thr Leu Ser Glu Lys Glu Lys Gln Val Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Gly Val Met Asp Asn Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Ala Cys Phe Ala Glu Glu Gly Pro Lys Phe Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Ala 
            580                 


<210>  38
<211>  584
<212>  PRT
<213>  Mus musculus

<400>  38

Glu Ala His Lys Ser Glu Ile Ala His Arg Tyr Asn Asp Leu Gly Glu 
1               5                   10                  15      


Gln His Phe Lys Gly Leu Val Leu Ile Ala Phe Ser Gln Tyr Leu Gln 
            20                  25                  30          


Lys Cys Ser Tyr Asp Glu His Ala Lys Leu Val Gln Glu Val Thr Asp 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Ala Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Ala Ile Pro Asn Leu 
65                  70                  75                  80  


Arg Glu Asn Tyr Gly Glu Leu Ala Asp Cys Cys Thr Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Ser Leu 
            100                 105                 110         


Pro Pro Phe Glu Arg Pro Glu Ala Glu Ala Met Cys Thr Ser Phe Lys 
        115                 120                 125             


Glu Asn Pro Thr Thr Phe Met Gly His Tyr Leu His Glu Val Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Tyr Tyr Ala Glu Gln 
145                 150                 155                 160 


Tyr Asn Glu Ile Leu Thr Gln Cys Cys Ala Glu Ala Asp Lys Glu Ser 
                165                 170                 175     


Cys Leu Thr Pro Lys Leu Asp Gly Val Lys Glu Lys Ala Leu Val Ser 
            180                 185                 190         


Ser Val Arg Gln Arg Met Lys Cys Ser Ser Met Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Thr Phe Pro 
    210                 215                 220                 


Asn Ala Asp Phe Ala Glu Ile Thr Lys Leu Ala Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val Asn Lys Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Glu Leu Ala Lys Tyr Met Cys Glu Asn Gln Ala Thr Ile Ser 
            260                 265                 270         


Ser Lys Leu Gln Thr Cys Cys Asp Lys Pro Leu Leu Lys Lys Ala His 
        275                 280                 285             


Cys Leu Ser Glu Val Glu His Asp Thr Met Pro Ala Asp Leu Pro Ala 
    290                 295                 300                 


Ile Ala Ala Asp Phe Val Glu Asp Gln Glu Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Thr Phe Leu Tyr Glu Tyr Ser Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Ser Leu Leu Leu Arg Leu Ala Lys Lys 
            340                 345                 350         


Tyr Glu Ala Thr Leu Glu Lys Cys Cys Ala Glu Ala Asn Pro Pro Ala 
        355                 360                 365             


Cys Tyr Gly Thr Val Leu Ala Glu Phe Gln Pro Leu Val Glu Glu Pro 
    370                 375                 380                 



Lys Asn Leu Val Lys Thr Asn Cys Asp Leu Tyr Glu Lys Leu Gly Glu 
385                 390                 395                 400 


Tyr Gly Phe Gln Asn Ala Ile Leu Val Arg Tyr Thr Gln Lys Ala Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Ala Ala Arg Asn Leu Gly Arg 
            420                 425                 430         


Val Gly Thr Lys Cys Cys Thr Leu Pro Glu Asp Gln Arg Leu Pro Cys 
        435                 440                 445             


Val Glu Asp Tyr Leu Ser Ala Ile Leu Asn Arg Val Cys Leu Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Glu His Val Thr Lys Cys Cys Ser Gly Ser 
465                 470                 475                 480 


Leu Val Glu Arg Arg Pro Cys Phe Ser Ala Leu Thr Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Lys Ala Glu Thr Phe Thr Phe His Ser Asp 
            500                 505                 510         


Ile Cys Thr Leu Pro Glu Lys Glu Lys Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Ala Glu Leu Val Lys His Lys Pro Lys Ala Thr Ala Glu Gln Leu 
    530                 535                 540                 


Lys Thr Val Met Asp Asp Phe Ala Gln Phe Leu Asp Thr Cys Cys Lys 
545                 550                 555                 560 


Ala Ala Asp Lys Asp Thr Cys Phe Ser Thr Glu Gly Pro Asn Leu Val 
                565                 570                 575     


Thr Arg Cys Lys Asp Ala Leu Ala 
            580                 


<210>  39
<211>  145
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Tencon25-ABDcon

<400>  39

Met Leu Pro Ala Pro Lys Asn Leu Val Val Ser Glu Val Thr Glu Asp 
1               5                   10                  15      


Ser Ala Arg Leu Ser Trp Thr Ala Pro Asp Ala Ala Phe Asp Ser Phe 
            20                  25                  30          


Leu Ile Gln Tyr Gln Glu Ser Glu Lys Val Gly Glu Ala Ile Val Leu 
        35                  40                  45              


Thr Val Pro Gly Ser Glu Arg Ser Tyr Asp Leu Thr Gly Leu Lys Pro 
    50                  55                  60                  


Gly Thr Glu Tyr Thr Val Ser Ile Tyr Gly Val Lys Gly Gly His Arg 
65                  70                  75                  80  


Ser Asn Pro Leu Ser Ala Ile Phe Thr Thr Gly Gly Gly Gly Ser Gly 
                85                  90                  95      


Gly Gly Gly Ser Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu 
            100                 105                 110         


Lys Lys Ala Gly Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys 
        115                 120                 125             


Ala Lys Thr Val Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys 
    130                 135                 140                 


Ala 
145 


<210>  40
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  40

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  41
<211>  145
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Tencon-ABDCon fusion protein

<400>  41

Met Leu Pro Ala Pro Lys Asn Leu Val Val Ser Glu Val Thr Glu Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Trp Thr Ala Pro Asp Ala Ala Phe Asp Ser Phe 
            20                  25                  30          


Leu Ile Gln Tyr Gln Glu Ser Glu Lys Val Gly Glu Ala Ile Asn Leu 
        35                  40                  45              


Thr Val Pro Gly Ser Glu Arg Ser Tyr Asp Leu Thr Gly Leu Lys Pro 
    50                  55                  60                  


Gly Thr Glu Tyr Thr Val Ser Ile Tyr Gly Val Lys Gly Gly His Arg 
65                  70                  75                  80  


Ser Asn Pro Leu Ser Ala Glu Phe Thr Thr Gly Gly Gly Gly Ser Gly 
                85                  90                  95      


Gly Gly Gly Ser Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu 
            100                 105                 110         


Lys Lys Ala Gly Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys 
        115                 120                 125             


Ala Lys Thr Val Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys 
    130                 135                 140                 


Ala 
145 


<210>  42
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal extension

<400>  42

Thr Ile Asp Gln Trp Leu 
1               5       


<210>  43
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal extension

<400>  43

Thr Ile Asp Glu Trp Leu 
1               5       


<210>  44
<211>  51
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ABDCon12

<400>  44

Thr Ile Asp Glu Trp Leu Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu 
1               5                   10                  15      


Glu Leu Lys Lys Ala Gly Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile 
            20                  25                  30          


Asn Lys Ala Lys Thr Val Glu Gly Val Asn Ala Leu Lys Asp Glu Ile 
        35                  40                  45              


Leu Lys Ala 
    50      


<210>  45
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal extension

<400>  45

Ala Pro Ala Val Asp Val 
1               5       


<210>  46
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal extension

<400>  46

Ile Ala Lys Glu Lys Ala 
1               5       


<210>  47
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  47

Val Pro Ala Ala Asp Val 
1               5       


<210>  48
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  48

Thr Val Lys Ser Ile Glu 
1               5       


<210>  49
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  49

Thr Pro Ala Val Asp Ala 
1               5       


<210>  50
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  50

Thr Leu Lys Ser Ile Lys 
1               5       


<210>  51
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  51

Trp Glu Lys Ala Ala Ala 
1               5       


<210>  52
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  52

Ala Val Asp Ala Asn Ser 
1               5       


<210>  53
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  53

Gln Leu Ala Ala Glu Ala 
1               5       


<210>  54
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  54

Ala Leu Lys Ala Ala Ala 
1               5       


<210>  55
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension

<400>  55

Glu Lys Leu Ala Ala Ala 
1               5       


