                         SEQUENCE LISTING

<110>  Christophe Poirier
       Poirier, Christophe
 
<120>  METHODS AND VECTORS FOR GENE TARGETING

<130>  GRU 2011-016 PCT

<150>  61/650,291
<151>  2012-05-22

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  1
aaggtaccgg agagttcaac acggatgg                                          28


<210>  2
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  2
ttaagcttcc tgcccttgct ctgcctgg                                          28


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  3
ggaaacagct atgaccatg                                                    19


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  4
gagcatatgg gattgaaagg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  5
aaattaaccc tcactaaagg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  6
accaacacac agtgtcatgg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  7
cagaatcaag ctcaacgtgg                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  8
tccctgtcga aggactctgg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  9
ccaaccagag gccccaccat                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  10
ggctaacctt gttcttcagg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA PRIMER

<400>  11
aaacgccatc aagtacctgg                                                   20


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  12
tcggtgcaga atagtctcgt t                                                 21


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  13
ccctgatttg tgcaatgagg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  14
tgggttcatc ctcgcagagg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  15
tcatgccgac ccgcagtccc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  16
gttaagagat catctccacc                                                   20


<210>  17
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  17
ggtaccctct tcttgcatct gtctgtgac                                         29


<210>  18
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  18
agatctgtga ctctggatga tcagtggc                                          28


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  19
gcccaggagt caggctaacc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  20
acacactcag atgtctcacc                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  21
ttggggtctt cacaggtgcc                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  22
gggttcccag ctcgccagcc                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  23
ccgcgagagc cgtctctagg                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  24
agcctcgggc acatgaaagg                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  25
gaacccgaga aaggcaaagg                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  26
tgttaactgt acctttgcgg                                                   20


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA PRIMER

<400>  27
ccacaggaag cagctgcatg g                                                 21


<210>  28
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  28
aattaattaa gctcgcgata agaggagcc                                         29


<210>  29
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  29
aattaattaa ggcacacaga cctggtgcag ggcggtgagt cc                          42


<210>  30
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  30
ttaattaaat gcagatggcg gacgcgaa                                          28


<210>  31
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  31
ttaattaatt ttattaggaa aggacagt                                          28


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  32
ccctttgtag cactggttgg                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  33
caattgttcc aggaaccagg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  34
gtcaagacta taagtacagg                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  35
gcggttccat cttccagcgg                                                   20


<210>  36
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  36
gatatctgat gagaggagct acctgc                                            26


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  37
gtcctcttca attttgcagc                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  38
gggttgggag gagtaggagg                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  39
ggttttgaag ataggagcgg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  40
ctagacacta ttccagatgg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  41
cactatccct ccttggctgg                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  42
tcattctaga atcagactgg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DNA PRIMER

<400>  43
ctagttggag agaagagcgg                                                   20


<210>  44
<211>  1966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC LUCIFERASE CONSTRUCT:KpnI-PacI-AscI-LUCIFERASE-PvuII 
       CASSETTE

<400>  44
ggtaccttaa ttaaggcgcg ccggtactgt tggtaaagcc accatggaag acgccaaaaa       60

cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact      120

gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca      180

tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc      240

tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct      300

tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa      360

cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt      420

gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat      480

ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac      540

gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt      600

cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa      660

aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat      720

ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg      780

ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta      840

tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct      900

gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt      960

atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc     1020

ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac     1080

atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt     1140

tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca     1200

aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga     1260

agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg     1320

ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg     1380

ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga     1440

cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt     1500

tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt     1560

aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct     1620

taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg     1680

aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag acatgataag     1740

atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg     1800

tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa     1860

caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta     1920

aagcaagtaa aacctctaca aatgtggtaa aatcgataag cagctg                    1966


<210>  45
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPN1 RESTRICTION ENDONUCLEASE SITE

<400>  45
ggtacc                                                                   6


<210>  46
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAC1 RESTRICTION ENDONUCLEASE SITE

<400>  46
ttaattaa                                                                 8


<210>  47
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ASC1 RESTRICTION ENDONUCLEASE SITE

<400>  47
ggcgcgcc                                                                 8


<210>  48
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVUII RESTRICTION ENDONUCLEASE SITE

<400>  48
cagctg                                                                   6


<210>  49
<211>  966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC FRT-TET-FRT CASSETTE: FRT-TETOCMVEX1-IN1(PARTIAL)-FRT

<400>  49
cgatcggaag ttcctatact ttctagagaa taggaacttc tcgggtgatc tgactgatcc       60

cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatctcga gtttaccact      120

ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga      180

aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt      240

accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga      300

tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc      360

gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc      420

gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat      480

cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag      540

cctccgcggc cccgaattcg agctcggtac ccggggatct acagctcttg atggaaacct      600

tctgcagaga acaatggccc ctctgggtta ttttaaactt cactctaagc cttctccacc      660

tgctctgctg tctctaattg ctccagaaac agcgtcattc acaactggat tattaatgaa      720

ctgagcgggg agcctcgggc acatgaaagg ggacttcaga cttggggaag tcttcgaagg      780

acccaaccag aggtgggttt tgttgtttta agacaaggtc tcatgtggca cagacaggct      840

ggcctcaaat ccataagtat gcctgagagc tggatttaca tgtgtttgtt accacacctg      900

gtactggcac acatgcaggg tactgaagtt cctatacttt ctagagaata ggaacttcgg      960

cgcgcc                                                                 966


<210>  50
<211>  820
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC FRT-TET-FRT CASSETTE: FRT-TETOCMVEX1-(PARTIAL)-FRT

<400>  50
cgatcggaag ttcctatact ttctagagaa taggaacttc tcgggtgatc tgactgatcc       60

cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatctcga gtttaccact      120

ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga      180

aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt      240

accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga      300

tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc      360

gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc      420

gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat      480

cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag      540

cctccgcggc cccgaattcg agctcggtac ccggggatct acagctcttg atggaaacct      600

tctgcagaga acaatggccc ctctgggtta ttttaaactt cactctaagc cttctccacc      660

tgctctgctg tctctaattg ctccagaaac agcgtcattc acaactggat tattaatgaa      720

ctgagcgggg agcctcgggc acatgaaagg ggacttcaga cttggggaag ttaattaaga      780

agttcctata ctttctagag aataggaact tcggcgcgcc                            820


<210>  51
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RESTRICTION ENDONUCLEASE SITE

<400>  51
cgatcg                                                                   6


<210>  52
<211>  1781
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DEST CASSETTE WITH+ORIENTATION

<400>  52
ggcgcgccca agctggctag gtaagcttga tcaacaagtt tgtacaaaaa agctgaacga       60

gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact      120

acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag      180

gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccggcga      240

gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg      300

atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta      360

cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata      420

agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg      480

aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt      540

acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg      600

atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg      660

cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga      720

gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca      780

ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc      840

atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact      900

gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac      960

agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc     1020

gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga     1080

cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc     1140

atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg     1200

atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac     1260

tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt     1320

ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag     1380

tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga     1440

tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga     1500

agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt     1560

ctggggaata taaatgtcag gctccgttat acacagccag tctgcaggtc gaccatagtg     1620

actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta     1680

atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg     1740

atctagaggg cccgcggttc gaaggtaagc ctaggcgcgc c                         1781


<210>  53
<211>  1781
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHETIC DEST CASSETTE WITH - ORIENTATION

<400>  53
ggcgcgccta ggcttacctt cgaaccgcgg gccctctaga tcaaccactt tgtacaagaa       60

agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa      120

aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag      180

actggctgtg tataacggag cctgacattt atattcccca gaacatcagg ttaatggcgt      240

ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg      300

gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg      360

ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca      420

tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc      480

tctctctttt ataggtgtaa accttaaact gcatttcacc agtccctgtt ctcgtcagca      540

aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct      600

ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg      660

gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg      720

taatacgctg cttcatagca cacctctttt tgacatactt cgggtataca tatcagtata      780

tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa      840

gccggatcca gatctttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt      900

aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg      960

catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa     1020

gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga     1080

gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca     1140

cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca     1200

gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc     1260

ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag     1320

gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt     1380

taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg     1440

aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt     1500

gatttttttc tccattttag cttccttagc tcctgaaaat ctcgccggat cctaactcaa     1560

aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc     1620

cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa     1680

atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac     1740

aaacttgttg atcaagctta cctagccagc ttgggcgcgc c                         1781


