                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
       Pentelute, Bradley
       Liao, Xiaoli
       Rabideau, Amy
       Ling, Jingjing
       Policarpo,  Rocco
 
<120>  PROTEIN RETROSPLICING ENABLED BY A DOUBLE LIGATION REACTION

<130>  M0656.70276WO00

<140>  TBD
<141>  2013-05-21

<150>  61/649,421
<151>  2012-05-21

<150>  61/649,866
<151>  2012-05-21

<160>  115   

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid linked to the thioester -COSR

<400>  1

Gly Gly Gly Gly Xaa 
1               5   


<210>  2
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is any amino acid linked to the thioester -COSR

<400>  2

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is any amino acid linked to the thioester -COSR

<400>  3

Gly Gly Gly Gly Gly Gly Xaa 
1               5           


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  4

Gly Gly Gly Gly Xaa 
1               5   


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  5

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  6

Gly Gly Gly Gly Xaa 
1               5   


<210>  7
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  7

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  8

Gly Gly Gly Gly Gly Gly Xaa 
1               5           


<210>  9
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  9

Gly Gly Gly Gly Xaa Xaa 
1               5       


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  10

Gly Gly Gly Gly Gly Xaa Xaa 
1               5           


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  11

Gly Gly Gly Gly Gly Gly Xaa Xaa 
1               5               


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(7)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  12

Gly Gly Gly Gly Xaa Xaa Xaa 
1               5           


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(8)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  13

Gly Gly Gly Gly Gly Xaa Xaa Xaa 
1               5               


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(9)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  14

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa 
1               5                   


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(8)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  15

Gly Gly Gly Gly Xaa Xaa Xaa Xaa 
1               5               


<210>  16
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  16

Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa 
1               5                   


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  17

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(9)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  18

Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa 
1               5                   


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(10)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  19

Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  20
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(11)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  20

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa 
1               5                   10      


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(10)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  21

Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  22
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(11)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  22

Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10      


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(12)
<223>  Xaa is Gly, Phe, Ser or Leu linked to the thioester -COSR

<400>  23

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10          


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  24

Gly Gly Gly Gly Xaa 
1               5   


<210>  25
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  25

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  26

Gly Gly Gly Gly Gly Gly Xaa 
1               5           


<210>  27
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  27

Gly Gly Gly Gly Xaa Xaa 
1               5       


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  Xaa is a D-amino acid linked to the thioester COSR

<400>  28

Gly Gly Gly Gly Gly Xaa Xaa 
1               5           


<210>  29
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  29

Gly Gly Gly Gly Gly Gly Xaa Xaa 
1               5               


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(7)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  30

Gly Gly Gly Gly Xaa Xaa Xaa 
1               5           


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(8)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  31

Gly Gly Gly Gly Gly Xaa Xaa Xaa 
1               5               


<210>  32
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(9)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  32

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa 
1               5                   


<210>  33
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(8)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  33

Gly Gly Gly Gly Xaa Xaa Xaa Xaa 
1               5               


<210>  34
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  34

Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa 
1               5                   


<210>  35
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  35

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  36

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  37
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(9)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  37

Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa 
1               5                   


<210>  38
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(10)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  38

Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  39
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(11)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  39

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa 
1               5                   10      


<210>  40
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(10)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  40

Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  41
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(11)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  41

Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10      


<210>  42
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(12)
<223>  Xaa is a D-amino acid linked to the thioester -COSR

<400>  42

Gly Gly Gly Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10          


<210>  43
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Gly linked to Y, a non-amino acid chemical 
       entity which is linked to -COSR, a thioester

<400>  43

Gly Gly Gly Xaa 
1               


<210>  44
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly linked to Y, a non-amino acid chemical 
       entity which is linked to -COSR, a thioester

<400>  44

Gly Gly Gly Gly Xaa 
1               5   


<210>  45
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly linked to Y, a non-amino acid chemical 
       entity which is linked to -COSR, a thioester

<400>  45

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  46
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Gly linked to Y(a PEG unit)-COSR(thioester)

<400>  46

Gly Gly Gly Xaa 
1               


<210>  47
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly linked to Y(a PEG unit)-COSR(thioester)

<400>  47

Gly Gly Gly Gly Xaa 
1               5   


<210>  48
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly linked to Y(a PEG unit)-COSR(thioester)

<400>  48

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  49
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Phe attached to the thioester COSR

<400>  49

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  50
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  50

Lys Leu Pro Glu Thr Gly Gly 
1               5           


<210>  51
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is Leu attached to a protein

<400>  51

Xaa Pro Ser Thr Gly Gly 
1               5       


<210>  52
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is His6-SUMO-LFN- attached to Leu

<400>  52

Xaa Pro Ser Thr Gly Gly His His His His His His 
1               5                   10          


<210>  53
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is His6-SUMO-LFN-DTA- attached to Leu

<400>  53

Xaa Pro Ser Thr Gly Gly His His His His His 
1               5                   10      


<210>  54
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is His6-SUMO-LFN-DTA-EGFP- attached to L

<400>  54

Xaa Pro Ser Thr Gly Gly His His His His His His 
1               5                   10          


<210>  55
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN- attached to Gly

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Phe attached to thioester -COSR

<400>  55

Xaa Gly Gly Gly Gly Xaa 
1               5       


<210>  56
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN-DTA- attached to Gly

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Phe attached to thioester -COSR

<400>  56

Xaa Gly Gly Gly Gly Xaa 
1               5       


<210>  57
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is eGFP- attached to Gly

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Phe attached to thioester -COSR

<400>  57

Xaa Gly Gly Gly Gly Xaa 
1               5       


<210>  58
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is EGFP attached to Cys

<400>  58

Xaa Phe Arg Ala Leu Lys Ala Ala 
1               5               


<210>  59
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  59

Ala Phe Leu Pro Ser Thr Gly Gly 
1               5               


<210>  60
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly attached to thioester COSR

<400>  60

Gly Gly Gly Gly Xaa 
1               5   


<210>  61
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Any amino acid

<400>  61

Leu Pro Xaa Thr Gly 
1               5   


<210>  62
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  62

Leu Pro Ser Thr Gly Gly 
1               5       


<210>  63
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Any amino acid

<400>  63

Leu Pro Xaa Thr Gly Gly Gly Gly Gly 
1               5                   


<210>  64
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Any amino acid

<400>  64

Phe Pro Xaa Thr Gly 
1               5   


<210>  65
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Any amino acid

<400>  65

Ala Pro Xaa Thr Gly 
1               5   


<210>  66
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly attached to -MPA-LR-CONH2

<400>  66

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  67
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Phe attached to -MPA-LR-CONH2

<400>  67

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  68
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Ser attached to -MPA-LR-CONH2

<400>  68

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  69
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Leu attached to -MPA-LR-CONH2

<400>  69

Gly Gly Gly Gly Gly Xaa 
1               5       


<210>  70
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly attached to -MPA-LR-CONH2

<400>  70

Gly Gly Gly Gly Xaa 
1               5   


<210>  71
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Gly attached to -MPA-LR-CONH2

<400>  71

Gly Gly Gly Xaa 
1               


<210>  72
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa is Gly attached to -MPA-LL-CONH2

<400>  72

Gly Gly Gly Gly Gly Ser Gly Arg Glu Leu Glu Arg Xaa 
1               5                   10              


<210>  73
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa is Gly attached to -MPA-LL-CONH2

<400>  73

Gly Gly Gly Gly Gly Ser Gly Arg Glu Leu Glu Arg Xaa 
1               5                   10              


<210>  74
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Gly attached to CONH2

<400>  74

Lys Leu Pro Glu Thr Gly Xaa 
1               5           


<210>  75
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  75

Cys Phe Arg Ala Leu Lys Ala Ala 
1               5               


<210>  76
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  76

Gly Gly Gly Gly Gly Leu Arg Leu 
1               5               


<210>  77
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa is Phe attached to thioester -COSR

<400>  77

Lys Leu Pro Glu Thr Gly Gly Gly Gly Gly Xaa 
1               5                   10      


<210>  78
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is eGFP- attached to Leu

<220>
<221>  MISC_FEATURE
<222>  (10)..(0)
<223>  Xaa is Phe attached to thioester -COSR

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<400>  78

Xaa Pro Ser Thr Gly Gly Gly Gly Gly Xaa 
1               5                   10  


<210>  79
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  79

Lys Leu Pro Glu Thr Gly Gly Gly Gly Gly Phe Cys Phe Arg Ala Leu 
1               5                   10                  15      


Lys Ala Ala 
            


<210>  80
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is His6-SUMO-eGFP- attached to Leu

<400>  80

Xaa Pro Ser Thr Gly Gly His His His His His His 
1               5                   10          


<210>  81
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  81
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctgg                   49


<210>  82
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  82
ctaatggtgg tggtggtggt ggccgccggt gctcggcagc ttgtacagct cgtccatgc        59


<210>  83
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  83
gaaggagata tacatatgat cagcagccat catcatcatc                             40


<210>  84
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  84
ccaggaccag caacatccga acaattaaat agaggtg                                37


<210>  85
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  85
gtataaaatg acaagtataa gaaacgttaa gccaacagat gtagaag                     47


<210>  86
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  86
gacaggcgtt tgggaaaccc gtaaaatctt tgtag                                  35


<210>  87
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is His6-SUMO-LFN- attached to Leu

<400>  87

Xaa Pro Ser Thr Gly Gly His His His His His His 
1               5                   10          


<210>  88
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is His6-SUMO-LFN-DTA(C186S)- attached to Leu

<400>  88

Xaa Pro Ser Thr Gly Gly His His His His His His 
1               5                   10          


<210>  89
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa is Arg attached to thioester -COSR

<400>  89

Gly Gly Gly Gly Gly Ser Gly Arg Glu Leu Glu Xaa 
1               5                   10          


<210>  90
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is a protein attached to Leu

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is Gly attached to -linker-COSR(thioester)

<400>  90

Xaa Pro Ser Thr Gly Gly Gly Gly Xaa 
1               5                   


<210>  91
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly attached to an L-linker-COSR(thioester)

<400>  91

Gly Gly Gly Gly Xaa 
1               5       


<210>  92
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly attached to a D-linker-COSR(thioester)

<400>  92

Gly Gly Gly Gly Xaa 
1               5       


<210>  93
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN attached to Ser

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Arg attached to thioester -COSR

<400>  93

Xaa Gly Arg Glu Leu Glu Xaa 
1               5           


<210>  94
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is eGFP- attached to Leu

<400>  94

Xaa Pro Ser Thr Gly Gly His His His His His His 
1               5                   10          


<210>  95
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  95

Gly Gly Gly Gly Gly Leu Arg Leu 
1               5               


<210>  96
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN-DTA- attached to Leu

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is Phe attached to thioester COSR

<400>  96

Xaa Pro Ser Thr Gly Gly Gly Gly Gly Xaa 
1               5                   10  


<210>  97
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Gly attached to -COSR

<400>  97

Lys Leu Pro Glu Thr Gly Xaa 
1               5           


<210>  98
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Gly attached to thioester COSR

<400>  98

Lys Leu Pro Glu Thr Xaa 
1               5       


<210>  99
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is Ala attached to thioester -COSR

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa can be any naturally occurring amino acid

<400>  99

Gly Gly Gly Gly Gly Asn Asn Ala Xaa 
1               5                   


<210>  100
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  100

Asn Asn Ala Ala 
1               


<210>  101
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is SUMO- attached to Ala

<400>  101

Xaa Phe Leu Pro Ser Thr Gly Gly 
1               5               


<210>  102
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  102

Ala Phe Leu Pro Ser Thr Gly Gly 
1               5               


<210>  103
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa is Gly attached to thioester -COSR

<400>  103

Ala Phe Leu Pro Ser Thr Gly Gly Gly Gly Xaa 
1               5                   10      


<210>  104
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  104

Gly Gly Gly Gly Gly 
1               5   


<210>  105
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  105

Ala Phe Gly Gly Gly Gly Gly Gly 
1               5               


<210>  106
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly attached to 10FN3

<400>  106

Gly Gly Gly Gly Xaa 
1               5   


<210>  107
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Gly attached to -LRL-CONH2

<400>  107

Gly Gly Gly Xaa 
1               


<210>  108
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is eGFP attached to Leu

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is eGFP- attached to Leu

<400>  108

Xaa Pro Ser Thr Gly Gly Gly Gly Gly Leu Arg Leu 
1               5                   10          


<210>  109
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN- attached to Leu

<400>  109

Xaa Pro Ser Thr Gly Gly His His His His His His 
1               5                   10          


<210>  110
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly attached to an affibody

<400>  110

Gly Gly Gly Gly Xaa 
1               5   


<210>  111
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN- attached to Leu

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is Gly attached to an affibody

<400>  111

Xaa Pro Ser Thr Gly Gly Gly Gly Xaa 
1               5                   


<210>  112
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Gly attached to 2Dpico

<400>  112

Gly Gly Gly Gly Xaa 
1               5   


<210>  113
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN attached to Leu

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is Gly attached to -10FN3

<400>  113

Xaa Pro Ser Thr Gly Gly Gly Gly Xaa 
1               5                   


<210>  114
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is LFN attached to Leu

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is Gly attached to 2Dpic

<400>  114

Xaa Pro Ser Thr Gly Gly Gly Gly Xaa 
1               5                   


<210>  115
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  115

Ala Phe Leu Pro Ser Thr Gly Gly 
1               5               


